MD16G1075200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015491
Physical Location & Seq
Reverse (-)
5261502 .. 5262086
585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1075200.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGCCATCGCCGATCAAACTCCGGCAAAACAGCAAAGGGAAGAAACCCTAACCCTCGTTGACGACAGTGTTCGTCAAGCTCCCTCTAAACGCCAAAGGGACGAAATCCTCACCCTGGCTGAGGATTCCGTAGATCTCAAGCGTCAGAAACCCTACACCGACATACTTTCTCTCCTCGACGAAGAAGAAGTAGAACCTCCGGAAGATTTGTCCTCTTTAATCACAACCCTGCAGCAAGAAATCTCACCTGAACCTCTCAACGCATTCCCCAGCTCCGAAACCCTAGCCGCCGAGTCGGCTCTAGAAGGCTATGCCTCGTCTTCCTCTGGCTCTTCCACTCCTACTTCTTCCAATGCGTGTTTTTTGAAGGAGGATGAGTGCGAGAGGGTTATGAGACATCTTCTAGAAGCTTCCGACGATGAGCTTGGGATTCCGCAGAGGGAGGCTCACGGGTCCGATGAAGTGGAGGGTGATGGGTTTAAATTCGATGGTCTGGATGGGTTTTCATTTGGTGATGGGTTGTGGGAGCTAGAAGACGAAGCCGCCAACTACTATACCTTGGTGCAATCTGAACTGTTTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

21.44

Weight (kDa)

4.05

Isoelectric Point (pI)

64.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015491)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26920 AT1G69760
malus_domestica MD13G1074000.v1.1 MD16G1075200.v1.1
prunus_persica Prupe.1G272000_v2.0.a1
pyrus_communis pycom16g06530
rosa_chinensis RchiOBHm_Chr4g0440081
rosa_laevigata RLG00000006207
rosa_multiflora Rmu_sc0001685.1_g000006
rosa_roxburghii Rroxscaffold_5G00381020
rosa_rugosa Rorug04G0324200
rosa_samantha Rh4AG374900 Rh4BG387200 Rh4CG401700 Rh4DG381500
rosa_wichuraiana Rw4G032070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 3 cut(s) 288, 434, 543
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 482
AfiI CCNNNNNNNGG 2 cut(s) 115, 121
AgsI TTSAA 1 cut(s) 367
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 5 cut(s) 80, 273, 410, 424, 529
AluI AGCT 5 cut(s) 80, 273, 410, 424, 529
Alw26I GTCTC 1 cut(s) 388
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 1 cut(s) 482
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 4 cut(s) 103, 237, 482, 524
AvaII GGWCC 1 cut(s) 453
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 312, 540
BbvCI CCTCAGC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 244
BccI CCATC 5 cut(s) 14, 467, 482, 491, 509
BciT130I CCWGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 4 cut(s) 284, 302, 404, 530
BfmI CTRYAG 1 cut(s) 230
BglI GCCNNNNNGGC 1 cut(s) 296
BglII AGATCT 1 cut(s) 133
BisI GCNGC 3 cut(s) 233, 288, 543
BlsI GCNGC 3 cut(s) 234, 289, 544
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 454
BmrFI CCNGG 1 cut(s) 116
BpiI GAAGAC 2 cut(s) 312, 540
BplI GAGNNNNNCTC 2 cut(s) 430, 462
Bpu10I CCTNAGC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 114, 558
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 4 cut(s) 156, 186, 362, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 121
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 114, 558
BseGI GGATG 2 cut(s) 379, 502
BseLI CCNNNNNNNGG 2 cut(s) 115, 121
BseMII CTCAG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 164
BseXI GCAGC 1 cut(s) 244
BseYI CCCAGC 1 cut(s) 269
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 23, 200
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 2 cut(s) 115, 121
BsmAI GTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 263
BsnI GGCC 1 cut(s) 5
Bsp13I TCCGGA 1 cut(s) 199
Bsp143I GATC 2 cut(s) 13, 133
BspACI CCGC 3 cut(s) 288, 434, 543
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 112
BspEI TCCGGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 454
BspMAI CTGCAG 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 114, 558
BssMI GATC 2 cut(s) 13, 133
BssT1I CCWWGG 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 2 cut(s) 68, 576
Bst6I CTCTTC 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 2 cut(s) 379, 502
BstKTI GATC 2 cut(s) 16, 136
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 2 cut(s) 13, 133
BstMWI GCNNNNNNNGC 1 cut(s) 296
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstSFI CTRYAG 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 2 cut(s) 312, 540
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 379, 502
BtsIMutI CAGTG 1 cut(s) 73
Cfr13I GGNCC 1 cut(s) 453
CseI GACGC 1 cut(s) 131
DdeI CTNAG 1 cut(s) 120
DpnI GATC 2 cut(s) 15, 135
DpnII GATC 2 cut(s) 13, 133
DraI TTTAAA 1 cut(s) 481
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 558
Eco47I GGWCC 1 cut(s) 453
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
FaiI YATR 5 cut(s) 164, 312, 392, 555, 581
FaqI GGGAC 1 cut(s) 113
Fnu4HI GCNGC 3 cut(s) 233, 288, 543
FokI GGATG 2 cut(s) 386, 509
Fsp4HI GCNGC 3 cut(s) 233, 288, 543
FspBI CTAG 4 cut(s) 284, 302, 404, 530
GluI GCNGC 3 cut(s) 233, 288, 543
GsaI CCCAGC 1 cut(s) 273
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 23, 200
HgaI GACGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HindIII AAGCTT 1 cut(s) 408
HinfI GANTC 3 cut(s) 125, 293, 430
HpaII CCGG 2 cut(s) 23, 200
HphI GGTGA 4 cut(s) 103, 237, 482, 524
Hpy166II GTNNAC 1 cut(s) 61
Hpy188I TCNGA 5 cut(s) 147, 277, 415, 457, 571
Hpy188III TCNNGA 4 cut(s) 200, 302, 404, 494
Hpy8I GTNNAC 1 cut(s) 61
Hpy99I CGWCG 2 cut(s) 182, 419
HpyAV CCTTC 2 cut(s) 299, 361
HpyCH4III ACNGT 2 cut(s) 68, 576
HpyCH4V TGCA 2 cut(s) 232, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 296
HpyF3I CTNAG 1 cut(s) 120
Kpn2I TCCGGA 1 cut(s) 199
Kzo9I GATC 2 cut(s) 13, 133
LguI GCTCTTC 1 cut(s) 337
LmnI GCTCC 3 cut(s) 85, 278, 526
LpnPI CCDG 9 cut(s) 36, 101, 128, 213, 242, 261, 283, 312, 479
Lsp1109I GCAGC 1 cut(s) 244
MaeI CTAG 4 cut(s) 284, 302, 404, 530
MalI GATC 2 cut(s) 15, 135
MboI GATC 2 cut(s) 13, 133
MboII GAAGA 9 cut(s) 53, 194, 197, 215, 312, 324, 339, 392, 545
MflI RGATCY 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 482
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 1 cut(s) 438
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 199
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 218, 480
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 23, 200
MspR9I CCNGG 1 cut(s) 116
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 296
NdeII GATC 2 cut(s) 13, 133
NlaIV GGNNCC 1 cut(s) 454
NmeAIII GCCGAG 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 337
PctI GAATGC 1 cut(s) 263
PfeI GAWTC 2 cut(s) 125, 430
PkrI GCNGC 3 cut(s) 234, 289, 544
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 114
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 1 cut(s) 453
PstI CTGCAG 1 cut(s) 234
PsuI RGATCY 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 337
SaqAI TTAA 2 cut(s) 218, 480
SatI GCNGC 3 cut(s) 233, 288, 543
Sau3AI GATC 2 cut(s) 13, 133
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 116
SetI ASST 9 cut(s) 82, 199, 250, 256, 275, 412, 426, 531, 560
SfcI CTRYAG 1 cut(s) 230
SinI GGWCC 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 1 cut(s) 482
SsiI CCGC 3 cut(s) 288, 434, 543
SspMI CTAG 4 cut(s) 284, 302, 404, 530
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 2 cut(s) 68, 576
TaqI TCGA 2 cut(s) 177, 486
TasI AATT 1 cut(s) 482
TauI GCSGC 2 cut(s) 290, 545
TfiI GAWTC 2 cut(s) 125, 430
Tru1I TTAA 2 cut(s) 218, 480
Tru9I TTAA 2 cut(s) 218, 480
TscAI CASTG 1 cut(s) 73
TseI GCWGC 1 cut(s) 232
TspDTI ATGAA 2 cut(s) 474, 495
TspGWI ACGGA 1 cut(s) 118
TspRI CASTG 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 453
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 1 cut(s) 482
XbaI TCTAGA 2 cut(s) 301, 403
XspI CTAG 4 cut(s) 284, 302, 404, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.