MD13G1090300.v1.1

carotenoid cleavage dioxygenase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012126
Physical Location & Seq
Reverse (-)
6367672 .. 6370882
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1090300.v1.1.491

Sequence Viewer

Length: 1773 bp
ATGGATGCCTTCTCTTCCTCCTTCCTATCTGCTTTTCCTGCCTTCTCTACCCCCAAACTCAGCAGTATTTCCTCTATCAGAATGGAAGAAAGACCCAGCTCCAAACCACCAGCTTCAAGGCCGCCGCCGTCGCAGCAGCCTCCGAGAACCACTCCACCACCGCCGCTAGCGGCAAAAGCCGATCATGCTCTTCAAAACAATGCCTCAACTTTCACTGCGGCAAAACAAACAGTTTCTGCGCTGCCTGCAGTGATCTTCAATGCTTTGGACGATTTCATCAACAGCTTCATCGACCCTCCAGTGAAACCCTCAGTGGACCCCAGGCACGTGCTCTCCCACAACTTTGCTCCGGTTGAGGAGCTTCCGCCGACGGAGTGTGAGATCATACACGGCTCCCTGCCTCCGTGCCTCGACGGAGCCTACATTCGCAATGGCCCCAACCCGCAATACCTTCCGCGTGGGCCCTACCACCTGTTCGACGGGGACGGCATGCTTCACTCCGTTAGGATCTCCGGAGGCCGCGCGGTGCTCTGCAGCCGATATGTCAAGACCTACAAGTACACCGTTGAGCGTGACGCCCGCCACCCTATACTTCCCAACTTCTTCTCTAGCTTCAACGGCCTCACCGCTTCCGCCACACGTGGCGCTCTCTCCGCTGCCCGGGTCCTTACAGGCCAATACAATCCTGCAAATGGCATTGGTCTTGCAAACACAAGTTTGGCCTTCTTTGGCGACCGACTTTACGCTCTCGGCGAGTCCGATCTCCCTTACTCCCTCCGCCTGACGTCCAACGGAGATATCGAAACTCTGGGACGCCATGATTTCGATGGCAAGCTCTCCATGAACATGACAGCTCATCCCAAGATAGACCCTGACACGGGGGAGGCCTTCGCCTTCCGCTATGGCTTTATACGGCCGTTTCTAACCTACTTCCGCTTCGATTCGAATGGCGTGAAGCAGCCGGACGTTCCGATATTCTCCATGGTCACTCCAACGTTCCTCCATGACTTTGCAATCACCAAAAAACATGCCATATTTGCTGACATACAAATTGGGTTGAACCTTATTGACATGATCACTAAACGAGCCACCCCATTTGGTTTGGACCCCTCGAAAGTGCCAAGAATTGGAGTGATTCCGCTGTACGCAAAGGACGAGTCGGAGATGAGGTGGTTTGAGGTGCCGGGTTTCAACGGCGTACACGCCACCAATGCATGGGATGAGGACGATGCTATTGTGATGGTGGCACCAAACGTTTTGTCAGCAGAGCACGTACTGGAGAGGGTGGATCTTGTTCATTGCTTGGTTGAGAAGGTTCGGATTGATCTCAAAACGGGGATTGTGACGAGGCAGCCGATTTCGACCAGAAATCTGGATTTCGCCGTGATCAATCCGGCTTACCTGGGGAGGAAGAATAAGTATGTGTATGCAGCTGAGGGTGATCCCATGCCTAAGATATCAGGAGTTGTAAAATTGGATGTGTCCAATGTGGAGCACAAGGAGTGTATTGTGGCCAGCAGGATGTTTGGGCCGGGGTGCTACGGGGGCGAGCCGTTTTTCGTGGCCAGAGAGCCCGAGAATCCTGAGGCGGACGAGGACAACGGGTTTTTGGTGTCGTATGTTCACGATGAGAAGGCCGGGGAATCGAGGTTCTTGGTGATGGATGCAAAGTCTCCTCAGCTTGACATTGTGGCTGCTGTGAGGATGCCCCGGCGGGTGCCTTATGGCTTCCACGGACTCTTTGTGAGGGAAAGTGATCTCAACAATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
RPE65

Protein Analysis

591

Amino Acids

65.01

Weight (kDa)

6.2

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 116 - 582 2.2e-117 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19170
fragaria_vesca FvH4_4g34690
malus_domestica MD13G1090300.v1.1 MD16G1090700.v1.1
prunus_persica Prupe.1G255500_v2.0.a1 Prupe.1G255500_v2.0.a1
pyrus_communis pycom13g07860 pycom16g07750
rosa_chinensis RchiOBHm_Chr4g0444051
rosa_laevigata RLG00000005862
rosa_multiflora Rmu_co8344017.1_g000001 Rmu_sc0015522.1_g000005
rosa_roxburghii Rroxscaffold_5G00384610
rosa_rugosa Rorug04G0349200
rosa_samantha Rh4AG410000 Rh4BG421100 Rh4CG435500 Rh4DG416400
rosa_wichuraiana Rw4G035220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 788
AccB1I GGYRCC 3 cut(s) 1180, 1246, 1717
AccB7I CCANNNNNTGG 2 cut(s) 1127, 1215
AccII CGCG 3 cut(s) 457, 522, 524
AccIII TCCGGA 1 cut(s) 512
AclI AACGTT 2 cut(s) 995, 1254
AclWI GGATC 3 cut(s) 515, 1296, 1436
AcoI YGGCCR 3 cut(s) 914, 1512, 1563
AcvI CACGTG 2 cut(s) 328, 641
AcyI GRCGYC 3 cut(s) 576, 785, 814
AfaI GTAC 4 cut(s) 560, 1145, 1200, 1275
AfiI CCNNNNNNNGG 6 cut(s) 660, 692, 877, 878, 1127, 1215
AflIII ACRYGT 1 cut(s) 638
AgsI TTSAA 6 cut(s) 117, 194, 259, 616, 1060, 1192
AjnI CCWGG 2 cut(s) 320, 1401
AloI GAACNNNNNNTCC 2 cut(s) 1634, 1666
AluBI AGCT 9 cut(s) 99, 113, 285, 361, 612, 835, 854, 1433, 1681
AluI AGCT 9 cut(s) 99, 113, 285, 361, 612, 835, 854, 1433, 1681
Alw21I GWGCWC 4 cut(s) 333, 531, 1272, 1497
Alw26I GTCTC 1 cut(s) 1677
AlwI GGATC 3 cut(s) 515, 1296, 1436
AlwNI CAGNNNCTG 1 cut(s) 236
Ama87I CYCGRG 2 cut(s) 660, 1574
Aor13HI TCCGGA 1 cut(s) 512
ApaI GGGCCC 1 cut(s) 465
ApeKI GCWGC 9 cut(s) 133, 136, 241, 534, 656, 958, 1351, 1430, 1694
ArsI GACNNNNNNTTYG 4 cut(s) 1142, 1174, 1244, 1276
AspLEI GCGC 3 cut(s) 241, 524, 647
AspS9I GGNCC 7 cut(s) 316, 434, 461, 462, 664, 1105, 1529
AsuC2I CCSGG 6 cut(s) 661, 662, 1185, 1533, 1639, 1711
AsuHPI GGTGA 4 cut(s) 616, 1009, 1451, 1669
AsuII TTCGAA 1 cut(s) 944
AsuNHI GCTAGC 1 cut(s) 166
AvaI CYCGRG 2 cut(s) 660, 1574
AvaII GGWCC 3 cut(s) 316, 664, 1105
AxyI CCTNAGG 1 cut(s) 1584
BaeGI GKGCMC 1 cut(s) 465
BalI TGGCCA 2 cut(s) 1514, 1565
BanI GGYRCC 3 cut(s) 1180, 1246, 1717
BanII GRGCYC 2 cut(s) 465, 1575
BbrPI CACGTG 2 cut(s) 328, 641
Bbv12I GWGCWC 4 cut(s) 333, 531, 1272, 1497
BbvCI CCTCAGC 2 cut(s) 1434, 1677
BbvI GCAGC 9 cut(s) 145, 148, 228, 546, 643, 970, 1363, 1442, 1681
BccI CCATC 3 cut(s) 821, 1234, 1654
BceAI ACGGC 9 cut(s) 112, 406, 502, 634, 901, 929, 1210, 1367, 1537
BciT130I CCWGG 2 cut(s) 322, 1403
BclI TGATCA 2 cut(s) 1074, 1386
BcnI CCSGG 6 cut(s) 661, 662, 1185, 1533, 1639, 1711
BcoDI GTCTC 1 cut(s) 1677
BfaI CTAG 2 cut(s) 167, 609
BfmI CTRYAG 2 cut(s) 246, 532
BfoI RGCGCY 1 cut(s) 648
Bme1390I CCNGG 8 cut(s) 322, 661, 662, 1185, 1403, 1533, 1639, 1711
Bme18I GGWCC 3 cut(s) 316, 664, 1105
BmeT110I CYCGRG 2 cut(s) 660, 1574
BmgT120I GGNCC 7 cut(s) 316, 434, 461, 462, 664, 1105, 1529
BmrFI CCNGG 8 cut(s) 322, 661, 662, 1185, 1403, 1533, 1639, 1711
BmsI GCATC 3 cut(s) 1219, 1654, 1695
BmtI GCTAGC 1 cut(s) 170
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BpmI CTGGAG 2 cut(s) 282, 1298
Bpu10I CCTNAGC 2 cut(s) 1434, 1677
Bpu14I TTCGAA 1 cut(s) 944
BpuMI CCSGG 6 cut(s) 661, 662, 1185, 1533, 1639, 1711
BsaAI YACGTR 3 cut(s) 328, 641, 1273
BsaHI GRCGYC 3 cut(s) 576, 785, 814
BsaJI CCNNGG 8 cut(s) 320, 660, 981, 1402, 1532, 1638, 1709, 1732
BsaWI WCCGGW 2 cut(s) 349, 512
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 1154, 1184
Bsc4I CCNNNNNNNGG 6 cut(s) 660, 692, 877, 878, 1127, 1215
Bse1I ACTGG 2 cut(s) 299, 1281
Bse21I CCTNAGG 1 cut(s) 1584
Bse3DI GCAATG 2 cut(s) 436, 1297
BseAI TCCGGA 1 cut(s) 512
BseBI CCWGG 2 cut(s) 322, 1403
BseDI CCNNGG 8 cut(s) 320, 660, 981, 1402, 1532, 1638, 1709, 1732
BseGI GGATG 7 cut(s) 10, 856, 1225, 1483, 1527, 1669, 1710
BseLI CCNNNNNNNGG 6 cut(s) 660, 692, 877, 878, 1127, 1215
BseMI GCAATG 2 cut(s) 436, 1297
BseMII CTCAG 5 cut(s) 73, 324, 1425, 1575, 1691
BseNI ACTGG 2 cut(s) 299, 1281
BseRI GAGGAG 2 cut(s) 371, 1665
BseSI GKGCMC 1 cut(s) 465
BseX3I CGGCCG 1 cut(s) 914
BseXI GCAGC 9 cut(s) 145, 148, 228, 546, 643, 970, 1363, 1442, 1681
BseYI CCCAGC 1 cut(s) 95
Bsh1236I CGCG 3 cut(s) 457, 522, 524
Bsh1285I CGRYCG 2 cut(s) 736, 917
BshNI GGYRCC 3 cut(s) 1180, 1246, 1717
BsiEI CGRYCG 2 cut(s) 736, 917
BsiHKAI GWGCWC 4 cut(s) 333, 531, 1272, 1497
BsiHKCI CYCGRG 2 cut(s) 660, 1574
BsiSI CCGG 9 cut(s) 350, 513, 661, 962, 1184, 1394, 1532, 1638, 1711
BslFI GGGAC 2 cut(s) 497, 825
BslI CCNNNNNNNGG 6 cut(s) 660, 692, 877, 878, 1127, 1215
BsmAI GTCTC 1 cut(s) 1677
BsmFI GGGAC 2 cut(s) 497, 825
BsoBI CYCGRG 2 cut(s) 660, 1574
Bsp119I TTCGAA 1 cut(s) 944
Bsp120I GGGCCC 1 cut(s) 461
Bsp1286I GDGCHC 6 cut(s) 333, 465, 531, 1272, 1497, 1575
Bsp13I TCCGGA 1 cut(s) 512
Bsp19I CCATGG 1 cut(s) 981
BspCNI CTCAG 5 cut(s) 72, 323, 1426, 1576, 1690
BspEI TCCGGA 1 cut(s) 512
BspFNI CGCG 3 cut(s) 457, 522, 524
BspMAI CTGCAG 2 cut(s) 250, 536
BspOI GCTAGC 1 cut(s) 170
BspPI GGATC 3 cut(s) 515, 1296, 1436
BspQI GCTCTTC 1 cut(s) 195
BspT104I TTCGAA 1 cut(s) 944
BspT107I GGYRCC 3 cut(s) 1180, 1246, 1717
BsrDI GCAATG 2 cut(s) 436, 1297
BsrI ACTGG 2 cut(s) 299, 1281
BssECI CCNNGG 8 cut(s) 320, 660, 981, 1402, 1532, 1638, 1709, 1732
BssNI GRCGYC 3 cut(s) 576, 785, 814
BssT1I CCWWGG 1 cut(s) 981
Bst2UI CCWGG 2 cut(s) 322, 1403
Bst4CI ACNGT 2 cut(s) 232, 565
Bst6I CTCTTC 2 cut(s) 19, 195
BstACI GRCGYC 3 cut(s) 576, 785, 814
BstBAI YACGTR 3 cut(s) 328, 641, 1273
BstBI TTCGAA 1 cut(s) 944
BstC8I GCNNGC 7 cut(s) 168, 246, 491, 580, 833, 1516, 1550
BstDEI CTNAG 6 cut(s) 59, 310, 1434, 1452, 1584, 1677
BstDSI CCRYGG 2 cut(s) 981, 1732
BstF5I GGATG 7 cut(s) 10, 856, 1225, 1483, 1527, 1669, 1710
BstFNI CGCG 3 cut(s) 457, 522, 524
BstH2I RGCGCY 1 cut(s) 648
BstHHI GCGC 3 cut(s) 241, 524, 647
BstMAI GTCTC 1 cut(s) 1677
BstMCI CGRYCG 2 cut(s) 736, 917
BstNI CCWGG 2 cut(s) 322, 1403
BstNSI RCATGY 2 cut(s) 493, 1031
BstSCI CCNGG 8 cut(s) 320, 659, 660, 1183, 1401, 1531, 1637, 1709
BstSFI CTRYAG 2 cut(s) 246, 532
BstSLI GKGCMC 1 cut(s) 465
BstUI CGCG 3 cut(s) 457, 522, 524
BstV1I GCAGC 9 cut(s) 145, 148, 228, 546, 643, 970, 1363, 1442, 1681
BstX2I RGATCY 2 cut(s) 507, 1288
BstXI CCANNNNNNTGG 1 cut(s) 1372
BstYI RGATCY 2 cut(s) 507, 1288
BstZI CGGCCG 1 cut(s) 914
Bsu36I CCTNAGG 1 cut(s) 1584
BtgI CCRYGG 2 cut(s) 981, 1732
BtsCI GGATG 7 cut(s) 10, 856, 1225, 1483, 1527, 1669, 1710
BtsI GCAGTG 2 cut(s) 213, 255
BtsIMutI CAGTG 4 cut(s) 213, 255, 306, 318
Cac8I GCNNGC 7 cut(s) 168, 246, 491, 580, 833, 1516, 1550
CaiI CAGNNNCTG 1 cut(s) 236
CfoI GCGC 3 cut(s) 241, 524, 647
Cfr13I GGNCC 7 cut(s) 316, 434, 461, 462, 664, 1105, 1529
Cfr9I CCCGGG 1 cut(s) 660
CseI GACGC 2 cut(s) 584, 822
Csp6I GTAC 4 cut(s) 559, 1144, 1199, 1274
CspCI CAANNNNNGTGG 2 cut(s) 1078, 1113
CviQI GTAC 4 cut(s) 559, 1144, 1199, 1274
DdeI CTNAG 6 cut(s) 59, 310, 1434, 1452, 1584, 1677
EaeI YGGCCR 3 cut(s) 914, 1512, 1563
EagI CGGCCG 1 cut(s) 914
Eam1104I CTCTTC 2 cut(s) 19, 195
EarI CTCTTC 2 cut(s) 19, 195
EciI GGCGGA 4 cut(s) 354, 622, 767, 1604
EclXI CGGCCG 1 cut(s) 914
Eco130I CCWWGG 1 cut(s) 981
Eco147I AGGCCT 1 cut(s) 887
Eco24I GRGCYC 2 cut(s) 465, 1575
Eco32I GATATC 2 cut(s) 799, 1458
Eco47I GGWCC 3 cut(s) 316, 664, 1105
Eco52I CGGCCG 1 cut(s) 914
Eco72I CACGTG 2 cut(s) 328, 641
Eco81I CCTNAGG 1 cut(s) 1584
Eco88I CYCGRG 2 cut(s) 660, 1574
EcoO109I RGGNCCY 2 cut(s) 462, 664
EcoRII CCWGG 2 cut(s) 320, 1401
EcoRV GATATC 2 cut(s) 799, 1458
EcoT14I CCWWGG 1 cut(s) 981
EcoT22I ATGCAT 1 cut(s) 1216
EcoT38I GRGCYC 2 cut(s) 465, 1575
ErhI CCWWGG 1 cut(s) 981
FaqI GGGAC 2 cut(s) 497, 825
FauI CCCGC 3 cut(s) 450, 587, 1707
FbaI TGATCA 2 cut(s) 1074, 1386
FokI GGATG 7 cut(s) 17, 843, 1232, 1490, 1534, 1676, 1717
FriOI GRGCYC 2 cut(s) 465, 1575
FspBI CTAG 2 cut(s) 167, 609
GlaI GCGC 3 cut(s) 240, 523, 646
GsaI CCCAGC 1 cut(s) 99
GsuI CTGGAG 2 cut(s) 282, 1298
HaeII RGCGCY 1 cut(s) 648
HapII CCGG 9 cut(s) 350, 513, 661, 962, 1184, 1394, 1532, 1638, 1711
HgaI GACGC 2 cut(s) 584, 822
HhaI GCGC 3 cut(s) 241, 524, 647
Hin1I GRCGYC 3 cut(s) 576, 785, 814
Hin6I GCGC 3 cut(s) 239, 522, 645
HinP1I GCGC 3 cut(s) 239, 522, 645
HinfI GANTC 7 cut(s) 755, 941, 1135, 1157, 1579, 1643, 1737
HpaII CCGG 9 cut(s) 350, 513, 661, 962, 1184, 1394, 1532, 1638, 1711
HphI GGTGA 4 cut(s) 616, 1009, 1451, 1669
Hpy166II GTNNAC 4 cut(s) 316, 561, 1201, 1624
Hpy188I TCNGA 6 cut(s) 80, 144, 760, 972, 1162, 1320
Hpy188III TCNNGA 6 cut(s) 513, 547, 1373, 1461, 1583, 1625
Hpy8I GTNNAC 4 cut(s) 316, 561, 1201, 1624
Hpy99I CGWCG 4 cut(s) 133, 373, 416, 482
HpyAV CCTTC 9 cut(s) 19, 31, 52, 461, 733, 898, 904, 1306, 1627
HpyCH4III ACNGT 2 cut(s) 232, 565
HpyCH4IV ACGT 7 cut(s) 327, 640, 785, 966, 995, 1254, 1272
HpyCH4V TGCA 8 cut(s) 248, 534, 689, 707, 1013, 1214, 1430, 1667
HpyF3I CTNAG 6 cut(s) 59, 310, 1434, 1452, 1584, 1677
HpySE526I ACGT 7 cut(s) 327, 640, 785, 966, 995, 1254, 1272
Hsp92I GRCGYC 3 cut(s) 576, 785, 814
HspAI GCGC 3 cut(s) 239, 522, 645
Kpn2I TCCGGA 1 cut(s) 512
Ksp22I TGATCA 2 cut(s) 1074, 1386
LguI GCTCTTC 1 cut(s) 195
LmnI GCTCC 6 cut(s) 104, 352, 358, 398, 416, 1492
Lsp1109I GCAGC 9 cut(s) 145, 148, 228, 546, 643, 970, 1363, 1442, 1681
LweI GCATC 3 cut(s) 1219, 1654, 1695
MaeI CTAG 2 cut(s) 167, 609
MaeII ACGT 7 cut(s) 327, 640, 785, 966, 995, 1254, 1272
MaeIII GTNAC 3 cut(s) 572, 985, 1342
MboII GAAGA 6 cut(s) 6, 98, 182, 247, 595, 1423
MflI RGATCY 2 cut(s) 507, 1288
MhlI GDGCHC 6 cut(s) 333, 465, 531, 1272, 1497, 1575
MlsI TGGCCA 2 cut(s) 1514, 1565
MluCI AATT 4 cut(s) 1050, 1125, 1472, 1765
MluNI TGGCCA 2 cut(s) 1514, 1565
MlyI GAGTC 3 cut(s) 764, 1166, 1731
MmeI TCCRAC 3 cut(s) 813, 1016, 1140
Mox20I TGGCCA 2 cut(s) 1514, 1565
Mph1103I ATGCAT 1 cut(s) 1216
MroI TCCGGA 1 cut(s) 512
MscI TGGCCA 2 cut(s) 1514, 1565
MslI CAYNNNNRTG 1 cut(s) 845
Msp20I TGGCCA 2 cut(s) 1514, 1565
MspA1I CMGCKG 3 cut(s) 656, 1141, 1433
MspI CCGG 9 cut(s) 350, 513, 661, 962, 1184, 1394, 1532, 1638, 1711
MspR9I CCNGG 8 cut(s) 322, 661, 662, 1185, 1403, 1533, 1639, 1711
MvaI CCWGG 2 cut(s) 322, 1403
MvnI CGCG 3 cut(s) 457, 522, 524
NciI CCSGG 6 cut(s) 661, 662, 1185, 1533, 1639, 1711
NcoI CCATGG 1 cut(s) 981
NheI GCTAGC 1 cut(s) 166
NmeAIII GCCGAG 1 cut(s) 729
NmuCI GTSAC 3 cut(s) 572, 985, 1342
NsiI ATGCAT 1 cut(s) 1216
NspI RCATGY 2 cut(s) 493, 1031
NspV TTCGAA 1 cut(s) 944
PaeI GCATGC 1 cut(s) 493
PceI AGGCCT 1 cut(s) 887
PciSI GCTCTTC 1 cut(s) 195
PcsI WCGNNNNNNNCGW 5 cut(s) 750, 756, 798, 1152, 1352
PfeI GAWTC 4 cut(s) 941, 1135, 1579, 1643
PflMI CCANNNNNTGG 2 cut(s) 1127, 1215
PleI GAGTC 3 cut(s) 763, 1165, 1731
PmaCI CACGTG 2 cut(s) 328, 641
PmlI CACGTG 2 cut(s) 328, 641
PpsI GAGTC 3 cut(s) 763, 1165, 1731
Ppu21I YACGTR 3 cut(s) 328, 641, 1273
PpuMI RGGWCCY 1 cut(s) 664
Psp1406I AACGTT 2 cut(s) 995, 1254
Psp5II RGGWCCY 1 cut(s) 664
Psp6I CCWGG 2 cut(s) 320, 1401
PspCI CACGTG 2 cut(s) 328, 641
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 2 cut(s) 320, 1401
PspOMI GGGCCC 1 cut(s) 461
PspPI GGNCC 7 cut(s) 316, 434, 461, 462, 664, 1105, 1529
PspPPI RGGWCCY 1 cut(s) 664
PstI CTGCAG 2 cut(s) 250, 536
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 2 cut(s) 507, 1288
PvuII CAGCTG 1 cut(s) 1433
RsaI GTAC 4 cut(s) 560, 1145, 1200, 1275
RsaNI GTAC 4 cut(s) 559, 1144, 1199, 1274
RseI CAYNNNNRTG 1 cut(s) 845
SapI GCTCTTC 1 cut(s) 195
Sau96I GGNCC 7 cut(s) 316, 434, 461, 462, 664, 1105, 1529
SchI GAGTC 3 cut(s) 764, 1166, 1731
ScrFI CCNGG 8 cut(s) 322, 661, 662, 1185, 1403, 1533, 1639, 1711
SduI GDGCHC 6 cut(s) 333, 465, 531, 1272, 1497, 1575
SfaNI GCATC 3 cut(s) 1219, 1654, 1695
SfcI CTRYAG 2 cut(s) 246, 532
SfuI TTCGAA 1 cut(s) 944
SinI GGWCC 3 cut(s) 316, 664, 1105
SmaI CCCGGG 1 cut(s) 662
SmiMI CAYNNNNRTG 1 cut(s) 845
SphI GCATGC 1 cut(s) 493
Sse9I AATT 4 cut(s) 1050, 1125, 1472, 1765
SseBI AGGCCT 1 cut(s) 887
SspMI CTAG 2 cut(s) 167, 609
StuI AGGCCT 1 cut(s) 887
StyD4I CCNGG 8 cut(s) 320, 659, 660, 1183, 1401, 1531, 1637, 1709
StyI CCWWGG 1 cut(s) 981
TaaI ACNGT 2 cut(s) 232, 565
TaiI ACGT 7 cut(s) 330, 643, 788, 969, 998, 1257, 1275
TaqII GACCGA 1 cut(s) 750
TasI AATT 4 cut(s) 1050, 1125, 1472, 1765
TatI WGTACW 1 cut(s) 558
TauI GCSGC 6 cut(s) 124, 127, 166, 173, 221, 522
TfiI GAWTC 4 cut(s) 941, 1135, 1579, 1643
TscAI CASTG 4 cut(s) 220, 255, 306, 318
TseFI GTSAC 3 cut(s) 572, 985, 1342
TseI GCWGC 9 cut(s) 133, 136, 241, 534, 656, 958, 1351, 1430, 1694
Tsp45I GTSAC 3 cut(s) 572, 985, 1342
TspDTI ATGAA 4 cut(s) 265, 277, 857, 1286
TspGWI ACGGA 6 cut(s) 386, 393, 429, 490, 807, 1749
TspMI CCCGGG 1 cut(s) 660
TspRI CASTG 4 cut(s) 220, 255, 306, 318
Van91I CCANNNNNTGG 2 cut(s) 1127, 1215
VpaK11BI GGWCC 3 cut(s) 316, 664, 1105
XceI RCATGY 2 cut(s) 493, 1031
XcmI CCANNNNNNNNNTGG 1 cut(s) 824
XmaI CCCGGG 1 cut(s) 660
XspI CTAG 2 cut(s) 167, 609
ZraI GACGTC 1 cut(s) 786
Zsp2I ATGCAT 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.