Rh4DG416400

carotenoid cleavage dioxygenase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012126
Physical Location & Seq
Forward (+)
62580921 .. 62582675
1755 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG416400.1

Sequence Viewer

Length: 1755 bp
ATGGATGCCTTGTCTTCTTCTTTCCTCTCCACATTGCCCACTCGATCTTCAACAACAGCACCAAAACTCACCCCAACCAAATCCCTCAACATCTCCTCTGTTAGAATTGAAGAAAGACCCACAACCCAAACACCTCCAAAAACCACAACACCACAACCCCCACCAAAACAGACTCCTCCTCCATCCTACTCCTCACCACCACCCACAAAACCTAATGCACCAGCACTTCCCGCTGTGATCTTCAACCTCTTCGACGACTTCATCAACAACTTCGTCGACCCGCCGGTCCGGTCTTCAGTCGACCCCAAACAGGCCCTCTCCGGTAACTTCGCTCCCGTCACTGAACTTCCCCCAACCGAATGTCAAGTAATCAAAGGCTCATTACCCTCCGCTCTCGACGGCGCTTACATCCGCAATGGCCCCAACCCTCAGTACCTCCCGCGCGGGCCCTACCACCTCTTCGACGGCGACGGCATGCTCCACTCCATCAGAATCTCCCAAGGCCGCGCCGTCCTCTGCAGCCGCTACGTCAAGACCTACAAGTACACCGTCGAGCGCGACGCCGGCTTCCCTCTCCTCCCCAACGTCTTCTCCGGCTTCAACGGCCTCACCGCCTCCGCGACACGTGGCGCCCTCTCCGCTGCCCGCGTGGCCTCGGGGCTGTACAATCCCGCCAACGGCATTGGCAACGCGAACACCAGCTTGGCGTTTTTCGGCGACCGTCTCTACGCGCTCGCCGAGTCTGATCTTCCTTACGCCGTGCGGGTGACGGCCGACGGCGACGTCGAGACCGTGGGACGGAACGACTTTAACGGCGAGCTGTTCATGAGCATGACGGCGCATCCGAAGATCGATCCAGACACCGGCGAGACCTTCGCTTTCCGTTACGGCCCGGTGCCGCCGTTTCTCACTTGCTTCCGGTTCGACCCGGCCGGAGCGAAACAACCCGACGTGCCGGTGTTCTCCCTGACCAGTCCTTCTATGCTCCACGACTTCGCGATCACTAAAAAGTACGCCGTGTTCACCGACATTCAGATCGGAATGAACCCGATGGAGATGATCGGCGGGGGATCTCCGGTGGGGTTGGACGCTTCTAAAGTATCTAGAATAGGAATAATTCCGAAATACGCGAGGGACGATTCCGAGATGAGGTGGTTTGAAGTGCCCGGGTTCAACATCATGCACGCGGTTAACGCATGGGACGAAGACGACGCCGTTGTGATGGTGGCGCCGAACATCTTATCGGCGGAGCACACTCTCGAGAGAATGGAGCTCGTCCACGCGCTCGTGGAGAAGGTGAGGATCGATCTCAAGACCGGGATCGTTTCGCGCCAACCGATCTCGACCCGGAATCTGGACTTCGCGGTGATAAACCCGGCTTACCAGGGGAAAAAGAACCGGTTCGTGTACGCGGGCGTCGGGGATCCGATGCCGAAGATATCGGGAGTGGTGAAGCTCGACGTGTCGGGTGATGAGCACAAGGAGAGCATAGTGGCGAGCAGGATGTACGGTCAGGGTTGCTTCGGGGGAGAGCCGTTTTTCGTGGCGAGGGAGCCGGAGAATCCGGAGGCGGAGGAGGACGACGGGTACGTGGTGTCGTATGTTCATGATGAGAAGACGGGAGAGTCGAGGTTTTTGGTGATGGACGCCAAGTCGTCTGAGCTGGAAACCGTGGCGGAAGTTAAGCTGCCGCGGCGGGTGCCGTACGGTTTTCACGGACTGTTTGTGAGGGAGAGTGACCTGAAGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
RPE65

Protein Analysis

584

Amino Acids

63.72

Weight (kDa)

5.7

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 111 - 576 4.6e-122 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19170
fragaria_vesca FvH4_4g34690
malus_domestica MD13G1090300.v1.1 MD16G1090700.v1.1
prunus_persica Prupe.1G255500_v2.0.a1 Prupe.1G255500_v2.0.a1
pyrus_communis pycom13g07860 pycom16g07750
rosa_chinensis RchiOBHm_Chr4g0444051
rosa_laevigata RLG00000005862
rosa_multiflora Rmu_co8344017.1_g000001 Rmu_sc0015522.1_g000005
rosa_roxburghii Rroxscaffold_5G00384610
rosa_rugosa Rorug04G0349200
rosa_samantha Rh4AG410000 Rh4BG421100 Rh4CG435500 Rh4DG416400
rosa_wichuraiana Rw4G035220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 284, 782, 1624
AatII GACGTC 1 cut(s) 786
AccB1I GGYRCC 4 cut(s) 629, 895, 1228, 1699
AccBSI CCGCTC 1 cut(s) 392
AccI GTMKAC 2 cut(s) 276, 300
AccIII TCCGGA 1 cut(s) 1564
AclWI GGATC 6 cut(s) 848, 1078, 1310, 1328, 1418, 1431
AcoI YGGCCR 2 cut(s) 771, 930
AcuI CTGAAG 1 cut(s) 279
AcvI CACGTG 1 cut(s) 626
AcyI GRCGYC 7 cut(s) 561, 630, 783, 1212, 1229, 1416, 1647
AfaI GTAC 8 cut(s) 434, 545, 665, 1013, 1409, 1508, 1589, 1706
AfiI CCNNNNNNNGG 6 cut(s) 310, 677, 798, 863, 1149, 1354
AflIII ACRYGT 2 cut(s) 623, 1461
AgeI ACCGGT 1 cut(s) 1398
AgsI TTSAA 6 cut(s) 51, 110, 244, 601, 1160, 1174
AhdI GACNNNNNGTC 1 cut(s) 1651
AjiI CACGTC 2 cut(s) 952, 1462
AjnI CCWGG 1 cut(s) 1383
AjuI GAANNNNNNNTTGG 2 cut(s) 686, 718
AluBI AGCT 6 cut(s) 702, 820, 1273, 1456, 1663, 1687
AluI AGCT 6 cut(s) 702, 820, 1273, 1456, 1663, 1687
Alw21I GWGCWC 3 cut(s) 1254, 1275, 1479
Alw26I GTCTC 3 cut(s) 728, 782, 863
AlwI GGATC 6 cut(s) 848, 1078, 1310, 1328, 1418, 1431
Ama87I CYCGRG 3 cut(s) 655, 1166, 1259
Aor13HI TCCGGA 1 cut(s) 1564
AoxI GGCC 9 cut(s) 312, 418, 446, 502, 604, 651, 771, 889, 930
ApaI GGGCCC 1 cut(s) 450
ApeKI GCWGC 3 cut(s) 519, 641, 1687
AsiGI ACCGGT 1 cut(s) 1398
Asp700I GAANNNNTTC 1 cut(s) 1400
AspS9I GGNCC 6 cut(s) 286, 313, 419, 446, 447, 890
AsuC2I CCSGG 7 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376
AvaI CYCGRG 3 cut(s) 655, 1166, 1259
AvaII GGWCC 1 cut(s) 286
BaeGI GKGCMC 2 cut(s) 450, 1167
BamHI GGATCC 1 cut(s) 1423
BanI GGYRCC 4 cut(s) 629, 895, 1228, 1699
BanII GRGCYC 2 cut(s) 450, 1275
BauI CACGAG 1 cut(s) 1286
BbrPI CACGTG 1 cut(s) 626
BbsI GAAGAC 5 cut(s) 6, 285, 580, 1212, 1622
Bbv12I GWGCWC 3 cut(s) 1254, 1275, 1479
BbvI GCAGC 3 cut(s) 531, 628, 1674
BccI CCATC 5 cut(s) 190, 494, 1045, 1216, 1636
BciT130I CCWGG 1 cut(s) 1385
BcnI CCSGG 7 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376
BcoDI GTCTC 3 cut(s) 728, 782, 863
BfaI CTAG 1 cut(s) 1104
BfmI CTRYAG 1 cut(s) 517
BfoI RGCGCY 3 cut(s) 405, 633, 1232
BglI GCCNNNNNGGC 1 cut(s) 650
BisI GCNGC 8 cut(s) 505, 520, 523, 642, 899, 1688, 1691, 1694
BlsI GCNGC 8 cut(s) 506, 521, 524, 643, 900, 1689, 1692, 1695
Bme1390I CCNGG 8 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376, 1385
Bme18I GGWCC 1 cut(s) 286
BmeRI GACNNNNNGTC 1 cut(s) 1651
BmeT110I CYCGRG 3 cut(s) 655, 1166, 1259
BmgBI CACGTC 2 cut(s) 952, 1462
BmgT120I GGNCC 6 cut(s) 286, 313, 419, 446, 447, 890
BmiI GGNNCC 8 cut(s) 421, 448, 631, 897, 1230, 1425, 1554, 1701
BmrFI CCNGG 8 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376, 1385
BmsI GCATC 2 cut(s) 850, 1419
BpiI GAAGAC 5 cut(s) 6, 285, 580, 1212, 1622
BpuEI CTTGAG 1 cut(s) 1295
BpuMI CCSGG 7 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376
Bsa29I ATCGAT 2 cut(s) 852, 1305
BsaAI YACGTR 2 cut(s) 626, 1591
BsaHI GRCGYC 7 cut(s) 561, 630, 783, 1212, 1229, 1416, 1647
BsaI GGTCTC 2 cut(s) 782, 863
BsaJI CCNNGG 7 cut(s) 499, 654, 792, 1166, 1384, 1671, 1691
BsaWI WCCGGW 6 cut(s) 288, 320, 918, 1075, 1398, 1564
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 575, 605
Bsc4I CCNNNNNNNGG 6 cut(s) 310, 677, 798, 863, 1149, 1354
Bse118I RCCGGY 5 cut(s) 283, 563, 863, 955, 1398
Bse1I ACTGG 1 cut(s) 972
Bse3DI GCAATG 2 cut(s) 32, 421
BseAI TCCGGA 1 cut(s) 1564
BseBI CCWGG 1 cut(s) 1385
BseCI ATCGAT 2 cut(s) 852, 1305
BseDI CCNNGG 7 cut(s) 499, 654, 792, 1166, 1384, 1671, 1691
BseGI GGATG 5 cut(s) 10, 182, 408, 841, 1509
BseLI CCNNNNNNNGG 6 cut(s) 310, 677, 798, 863, 1149, 1354
BseMI GCAATG 2 cut(s) 32, 421
BseMII CTCAG 2 cut(s) 443, 1650
BseNI ACTGG 1 cut(s) 972
BseRI GAGGAG 6 cut(s) 85, 165, 168, 181, 566, 1589
BseSI GKGCMC 2 cut(s) 450, 1167
BseX3I CGGCCG 2 cut(s) 771, 930
BseXI GCAGC 3 cut(s) 531, 628, 1674
Bsh1285I CGRYCG 3 cut(s) 721, 774, 933
BshFI GGCC 9 cut(s) 314, 420, 448, 504, 606, 653, 773, 891, 932
BshNI GGYRCC 4 cut(s) 629, 895, 1228, 1699
BshTI ACCGGT 1 cut(s) 1398
BshVI ATCGAT 2 cut(s) 852, 1305
BsiEI CGRYCG 3 cut(s) 721, 774, 933
BsiHKAI GWGCWC 3 cut(s) 1254, 1275, 1479
BsiHKCI CYCGRG 3 cut(s) 655, 1166, 1259
BsiWI CGTACG 1 cut(s) 1704
BslFI GGGAC 3 cut(s) 810, 1148, 1214
BslI CCNNNNNNNGG 6 cut(s) 310, 677, 798, 863, 1149, 1354
BsmAI GTCTC 3 cut(s) 728, 782, 863
BsmBI CGTCTC 1 cut(s) 728
BsmFI GGGAC 3 cut(s) 810, 1148, 1214
BsnI GGCC 9 cut(s) 314, 420, 448, 504, 606, 653, 773, 891, 932
Bso31I GGTCTC 2 cut(s) 782, 863
BsoBI CYCGRG 3 cut(s) 655, 1166, 1259
Bsp120I GGGCCC 1 cut(s) 446
Bsp1286I GDGCHC 5 cut(s) 450, 1167, 1254, 1275, 1479
Bsp13I TCCGGA 1 cut(s) 1564
Bsp1407I TGTACA 1 cut(s) 663
Bsp68I TCGCGA 1 cut(s) 998
BspANI GGCC 9 cut(s) 314, 420, 448, 504, 606, 653, 773, 891, 932
BspCNI CTCAG 2 cut(s) 442, 1651
BspDI ATCGAT 2 cut(s) 852, 1305
BspEI TCCGGA 1 cut(s) 1564
BspHI TCATGA 2 cut(s) 825, 1606
BspLI GGNNCC 8 cut(s) 421, 448, 631, 897, 1230, 1425, 1554, 1701
BspMAI CTGCAG 1 cut(s) 521
BspPI GGATC 6 cut(s) 848, 1078, 1310, 1328, 1418, 1431
BspT107I GGYRCC 4 cut(s) 629, 895, 1228, 1699
BspTNI GGTCTC 2 cut(s) 782, 863
BsrBI CCGCTC 1 cut(s) 392
BsrDI GCAATG 2 cut(s) 32, 421
BsrFI RCCGGY 5 cut(s) 283, 563, 863, 955, 1398
BsrGI TGTACA 1 cut(s) 663
BsrI ACTGG 1 cut(s) 972
BssAI RCCGGY 5 cut(s) 283, 563, 863, 955, 1398
BssECI CCNNGG 7 cut(s) 499, 654, 792, 1166, 1384, 1671, 1691
BssNI GRCGYC 7 cut(s) 561, 630, 783, 1212, 1229, 1416, 1647
BssSI CACGAG 1 cut(s) 1286
BssT1I CCWWGG 1 cut(s) 499
Bst2BI CACGAG 1 cut(s) 1286
Bst2UI CCWGG 1 cut(s) 1385
Bst4CI ACNGT 7 cut(s) 550, 722, 793, 1511, 1672, 1709, 1722
Bst6I CTCTTC 2 cut(s) 254, 464
BstACI GRCGYC 7 cut(s) 561, 630, 783, 1212, 1229, 1416, 1647
BstAUI TGTACA 1 cut(s) 663
BstBAI YACGTR 2 cut(s) 626, 1591
BstC8I GCNNGC 9 cut(s) 446, 476, 565, 646, 735, 818, 1185, 1414, 1498
BstDEI CTNAG 2 cut(s) 429, 1659
BstDSI CCRYGG 3 cut(s) 792, 1671, 1691
BstF5I GGATG 5 cut(s) 10, 182, 408, 841, 1509
BstH2I RGCGCY 3 cut(s) 405, 633, 1232
BstMAI GTCTC 3 cut(s) 728, 782, 863
BstMCI CGRYCG 3 cut(s) 721, 774, 933
BstMWI GCNNNNNNNGC 8 cut(s) 230, 564, 603, 638, 650, 1193, 1693, 1699
BstNI CCWGG 1 cut(s) 1385
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 8 cut(s) 891, 927, 1165, 1166, 1316, 1346, 1374, 1383
BstSFI CTRYAG 1 cut(s) 517
BstSLI GKGCMC 2 cut(s) 450, 1167
BstV1I GCAGC 3 cut(s) 531, 628, 1674
BstV2I GAAGAC 5 cut(s) 6, 285, 580, 1212, 1622
BstX2I RGATCY 2 cut(s) 1070, 1423
BstYI RGATCY 2 cut(s) 1070, 1423
BstZI CGGCCG 2 cut(s) 771, 930
Bsu15I ATCGAT 2 cut(s) 852, 1305
BsuRI GGCC 9 cut(s) 314, 420, 448, 504, 606, 653, 773, 891, 932
BsuTUI ATCGAT 2 cut(s) 852, 1305
BtgI CCRYGG 3 cut(s) 792, 1671, 1691
BtrI CACGTC 2 cut(s) 952, 1462
BtsCI GGATG 5 cut(s) 10, 182, 408, 841, 1509
BtsIMutI CAGTG 1 cut(s) 339
BtuMI TCGCGA 1 cut(s) 998
Cac8I GCNNGC 9 cut(s) 446, 476, 565, 646, 735, 818, 1185, 1414, 1498
CciI TCATGA 2 cut(s) 825, 1606
Cfr10I RCCGGY 5 cut(s) 283, 563, 863, 955, 1398
Cfr13I GGNCC 6 cut(s) 286, 313, 419, 446, 447, 890
Cfr42I CCGCGG 1 cut(s) 1694
Cfr9I CCCGGG 1 cut(s) 1166
ClaI ATCGAT 2 cut(s) 852, 1305
CpoI CGGWCCG 1 cut(s) 286
CseI GACGC 5 cut(s) 569, 1097, 1220, 1405, 1655
Csp6I GTAC 8 cut(s) 433, 544, 664, 1012, 1408, 1507, 1588, 1705
CspAI ACCGGT 1 cut(s) 1398
CspI CGGWCCG 1 cut(s) 286
CviAII CATG 6 cut(s) 475, 826, 832, 1180, 1197, 1607
CviQI GTAC 8 cut(s) 433, 544, 664, 1012, 1408, 1507, 1588, 1705
DdeI CTNAG 2 cut(s) 429, 1659
DinI GGCGCC 2 cut(s) 631, 1230
DrdI GACNNNNNNGTC 3 cut(s) 284, 782, 1624
DriI GACNNNNNGTC 1 cut(s) 1651
DseDI GACNNNNNNGTC 3 cut(s) 284, 782, 1624
EaeI YGGCCR 2 cut(s) 771, 930
EagI CGGCCG 2 cut(s) 771, 930
Eam1104I CTCTTC 2 cut(s) 254, 464
Eam1105I GACNNNNNGTC 1 cut(s) 1651
EarI CTCTTC 2 cut(s) 254, 464
EciI GGCGGA 3 cut(s) 1262, 1586, 1691
Ecl136II GAGCTC 1 cut(s) 1273
EclXI CGGCCG 2 cut(s) 771, 930
Eco130I CCWWGG 1 cut(s) 499
Eco24I GRGCYC 2 cut(s) 450, 1275
Eco31I GGTCTC 2 cut(s) 782, 863
Eco32I GATATC 1 cut(s) 1440
Eco47I GGWCC 1 cut(s) 286
Eco52I CGGCCG 2 cut(s) 771, 930
Eco53kI GAGCTC 1 cut(s) 1273
Eco57I CTGAAG 1 cut(s) 279
Eco72I CACGTG 1 cut(s) 626
Eco88I CYCGRG 3 cut(s) 655, 1166, 1259
EcoICRI GAGCTC 1 cut(s) 1273
EcoO109I RGGNCCY 2 cut(s) 313, 447
EcoRII CCWGG 1 cut(s) 1383
EcoRV GATATC 1 cut(s) 1440
EcoT14I CCWWGG 1 cut(s) 499
EcoT38I GRGCYC 2 cut(s) 450, 1275
EgeI GGCGCC 2 cut(s) 631, 1230
EheI GGCGCC 2 cut(s) 631, 1230
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 6 cut(s) 478, 829, 835, 1183, 1200, 1610
FaiI YATR 9 cut(s) 476, 827, 833, 983, 1181, 1198, 1490, 1602, 1608
FaqI GGGAC 3 cut(s) 810, 1148, 1214
FatI CATG 6 cut(s) 474, 825, 831, 1179, 1196, 1606
FblI GTMKAC 2 cut(s) 276, 300
Fnu4HI GCNGC 8 cut(s) 505, 520, 523, 642, 899, 1688, 1691, 1694
FokI GGATG 5 cut(s) 17, 169, 395, 828, 1516
FriOI GRGCYC 2 cut(s) 450, 1275
Fsp4HI GCNGC 8 cut(s) 505, 520, 523, 642, 899, 1688, 1691, 1694
FspBI CTAG 1 cut(s) 1104
GluI GCNGC 8 cut(s) 505, 520, 523, 642, 899, 1688, 1691, 1694
HaeII RGCGCY 3 cut(s) 405, 633, 1232
HaeIII GGCC 9 cut(s) 314, 420, 448, 504, 606, 653, 773, 891, 932
HgaI GACGC 5 cut(s) 569, 1097, 1220, 1405, 1655
Hin1I GRCGYC 7 cut(s) 561, 630, 783, 1212, 1229, 1416, 1647
Hin1II CATG 6 cut(s) 478, 829, 835, 1183, 1200, 1610
HincII GTYRAC 3 cut(s) 277, 301, 1192
HindII GTYRAC 3 cut(s) 277, 301, 1192
HinfI GANTC 7 cut(s) 172, 492, 740, 1139, 1351, 1561, 1625
HpaI GTTAAC 1 cut(s) 1192
Hpy166II GTNNAC 7 cut(s) 277, 301, 546, 1023, 1192, 1279, 1408
Hpy188I TCNGA 9 cut(s) 491, 745, 846, 1035, 1040, 1122, 1144, 1428, 1660
Hpy8I GTNNAC 7 cut(s) 277, 301, 546, 1023, 1192, 1279, 1408
HpyAV CCTTC 3 cut(s) 883, 987, 1288
HpyCH4III ACNGT 7 cut(s) 550, 722, 793, 1511, 1672, 1709, 1722
HpyCH4IV ACGT 7 cut(s) 528, 585, 625, 783, 951, 1461, 1590
HpyCH4V TGCA 3 cut(s) 218, 519, 1183
HpyF10VI GCNNNNNNNGC 8 cut(s) 230, 564, 603, 638, 650, 1193, 1693, 1699
HpyF3I CTNAG 2 cut(s) 429, 1659
HpySE526I ACGT 7 cut(s) 528, 585, 625, 783, 951, 1461, 1590
Hsp92I GRCGYC 7 cut(s) 561, 630, 783, 1212, 1229, 1416, 1647
Hsp92II CATG 6 cut(s) 478, 829, 835, 1183, 1200, 1610
KasI GGCGCC 2 cut(s) 629, 1228
Kpn2I TCCGGA 1 cut(s) 1564
KroI GCCGGC 1 cut(s) 563
KroNI GCCGGC 1 cut(s) 565
KspAI GTTAAC 1 cut(s) 1192
KspI CCGCGG 1 cut(s) 1694
LmnI GCTCC 7 cut(s) 337, 483, 935, 990, 1249, 1270, 1552
Lsp1109I GCAGC 3 cut(s) 531, 628, 1674
LweI GCATC 2 cut(s) 850, 1419
MaeI CTAG 1 cut(s) 1104
MaeII ACGT 7 cut(s) 528, 585, 625, 783, 951, 1461, 1590
MaeIII GTNAC 5 cut(s) 323, 337, 766, 884, 1736
MbiI CCGCTC 1 cut(s) 392
MflI RGATCY 2 cut(s) 1070, 1423
MhlI GDGCHC 5 cut(s) 450, 1167, 1254, 1275, 1479
MluCI AATT 2 cut(s) 105, 1116
Mly113I GGCGCC 2 cut(s) 630, 1229
MlyI GAGTC 3 cut(s) 166, 749, 1634
MmeI TCCRAC 1 cut(s) 1065
MroI TCCGGA 1 cut(s) 1564
MroNI GCCGGC 1 cut(s) 563
MroXI GAANNNNTTC 1 cut(s) 1400
MseI TTAA 3 cut(s) 810, 1191, 1683
MslI CAYNNNNRTG 1 cut(s) 830
MspA1I CMGCKG 3 cut(s) 233, 641, 1693
MspR9I CCNGG 8 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376, 1385
MvaI CCWGG 1 cut(s) 1385
MwoI GCNNNNNNNGC 8 cut(s) 230, 564, 603, 638, 650, 1193, 1693, 1699
NaeI GCCGGC 1 cut(s) 565
NarI GGCGCC 2 cut(s) 630, 1229
NciI CCSGG 7 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376
NgoMIV GCCGGC 1 cut(s) 563
NlaIII CATG 6 cut(s) 478, 829, 835, 1183, 1200, 1610
NlaIV GGNNCC 8 cut(s) 421, 448, 631, 897, 1230, 1425, 1554, 1701
NmeAIII GCCGAG 1 cut(s) 763
NmuCI GTSAC 3 cut(s) 337, 766, 1736
NruI TCGCGA 1 cut(s) 998
NspI RCATGY 1 cut(s) 478
PaeI GCATGC 1 cut(s) 478
PaeR7I CTCGAG 1 cut(s) 1259
PagI TCATGA 2 cut(s) 825, 1606
PcsI WCGNNNNNNNCGW 9 cut(s) 735, 783, 789, 842, 1134, 1200, 1334, 1587, 1625
PdiI GCCGGC 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 1400
PfeI GAWTC 4 cut(s) 492, 1139, 1351, 1561
Pfl23II CGTACG 1 cut(s) 1704
PinAI ACCGGT 1 cut(s) 1398
PkrI GCNGC 8 cut(s) 506, 521, 524, 643, 900, 1689, 1692, 1695
PleI GAGTC 3 cut(s) 166, 748, 1633
PluTI GGCGCC 2 cut(s) 633, 1232
PmaCI CACGTG 1 cut(s) 626
PmlI CACGTG 1 cut(s) 626
PpsI GAGTC 3 cut(s) 166, 748, 1633
Ppu21I YACGTR 2 cut(s) 626, 1591
Psp124BI GAGCTC 1 cut(s) 1275
Psp6I CCWGG 1 cut(s) 1383
PspCI CACGTG 1 cut(s) 626
PspGI CCWGG 1 cut(s) 1383
PspLI CGTACG 1 cut(s) 1704
PspN4I GGNNCC 8 cut(s) 421, 448, 631, 897, 1230, 1425, 1554, 1701
PspOMI GGGCCC 1 cut(s) 446
PspPI GGNCC 6 cut(s) 286, 313, 419, 446, 447, 890
PsrI GAACNNNNNNTAC 2 cut(s) 1004, 1036
PstI CTGCAG 1 cut(s) 521
PsuI RGATCY 2 cut(s) 1070, 1423
RruI TCGCGA 1 cut(s) 998
RsaI GTAC 8 cut(s) 434, 545, 665, 1013, 1409, 1508, 1589, 1706
RsaNI GTAC 8 cut(s) 433, 544, 664, 1012, 1408, 1507, 1588, 1705
RseI CAYNNNNRTG 1 cut(s) 830
Rsr2I CGGWCCG 1 cut(s) 286
RsrII CGGWCCG 1 cut(s) 286
SacI GAGCTC 1 cut(s) 1275
SacII CCGCGG 1 cut(s) 1694
SalI GTCGAC 2 cut(s) 275, 299
SaqAI TTAA 3 cut(s) 810, 1191, 1683
SatI GCNGC 8 cut(s) 505, 520, 523, 642, 899, 1688, 1691, 1694
Sau96I GGNCC 6 cut(s) 286, 313, 419, 446, 447, 890
SchI GAGTC 3 cut(s) 166, 749, 1634
ScrFI CCNGG 8 cut(s) 893, 929, 1167, 1168, 1318, 1348, 1376, 1385
SduI GDGCHC 5 cut(s) 450, 1167, 1254, 1275, 1479
SfaNI GCATC 2 cut(s) 850, 1419
SfcI CTRYAG 1 cut(s) 517
SfoI GGCGCC 2 cut(s) 631, 1230
Sfr274I CTCGAG 1 cut(s) 1259
Sfr303I CCGCGG 1 cut(s) 1694
SgrAI CRCCGGYG 1 cut(s) 863
SgrBI CCGCGG 1 cut(s) 1694
SinI GGWCC 1 cut(s) 286
SlaI CTCGAG 1 cut(s) 1259
SmaI CCCGGG 1 cut(s) 1168
SmiMI CAYNNNNRTG 1 cut(s) 830
SmlI CTYRAG 2 cut(s) 1259, 1310
SmoI CTYRAG 2 cut(s) 1259, 1310
SphI GCATGC 1 cut(s) 478
Sse9I AATT 2 cut(s) 105, 1116
SspDI GGCGCC 2 cut(s) 629, 1228
SspMI CTAG 1 cut(s) 1104
SstI GAGCTC 1 cut(s) 1275
StyD4I CCNGG 8 cut(s) 891, 927, 1165, 1166, 1316, 1346, 1374, 1383
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 7 cut(s) 550, 722, 793, 1511, 1672, 1709, 1722
TaiI ACGT 7 cut(s) 531, 588, 628, 786, 954, 1464, 1593
TasI AATT 2 cut(s) 105, 1116
TatI WGTACW 2 cut(s) 543, 663
TauI GCSGC 5 cut(s) 507, 525, 901, 1693, 1696
TfiI GAWTC 4 cut(s) 492, 1139, 1351, 1561
Tru1I TTAA 3 cut(s) 810, 1191, 1683
Tru9I TTAA 3 cut(s) 810, 1191, 1683
TscAI CASTG 1 cut(s) 346
TseFI GTSAC 3 cut(s) 337, 766, 1736
TseI GCWGC 3 cut(s) 519, 641, 1687
Tsp45I GTSAC 3 cut(s) 337, 766, 1736
TspDTI ATGAA 4 cut(s) 250, 814, 1058, 1595
TspGWI ACGGA 3 cut(s) 814, 872, 1731
TspMI CCCGGG 1 cut(s) 1166
TspRI CASTG 1 cut(s) 346
VpaK11BI GGWCC 1 cut(s) 286
XbaI TCTAGA 1 cut(s) 1103
XceI RCATGY 1 cut(s) 478
XhoI CTCGAG 1 cut(s) 1259
XmaI CCCGGG 1 cut(s) 1166
XmiI GTMKAC 2 cut(s) 276, 300
XmnI GAANNNNTTC 1 cut(s) 1400
XspI CTAG 1 cut(s) 1104
ZraI GACGTC 1 cut(s) 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.