Rroxscaffold_5G00384610

carotenoid cleavage dioxygenase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012126
Physical Location & Seq
Forward (+)
63902013 .. 63903407
1395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384610.1

Sequence Viewer

Length: 1395 bp
ATGTCAAGTAATCAAAGGCTCATTACCCTCCGCTCTCGACGGCGCTTACATCCGCAATGGCCCGAACCCTCAGTACCTCCCGCGCGGGCCCTACCACCTCTTCGACGGCGACGGCATGCTCCACTCCATCAGAATCTCCCAAGGCCGCGCCGTCCTCTGCAGCCGCTACGTCAAGACCTACAAGTACACCGTCGAGCGCGACGCCGGCTTCCTCTCCTCCCCAGCGTCTTCTCCGGCTTCAACGGCCTCACCGCCTCAGCGACACGTGGCGCCCTCTCCGCTGCCCGCGTGGCCTCGGGGCTGTACAACCCCGCCAACGGCATTGGCAACGCGAACACGAGCTTGGCGTTTTTCGGCGACCGTCTCTACGCGCTCGCCGAGTATGATCTTCCTTACGCAGTGCGGTTGACGGCCGACGGCGACGTCGAGACCGTGGGACGGAACGACTTTAACGGCGAGCTGTTCATGAGCATGACGGCGCATCCGAAGATCGATCCAGACACCGGCGAGACCTTCGCTTTCCGTTACGGCCCGGTGCCGCCGTTTCTGACTTACTTCCGGTTCGACCCGACGGGAGCGAAACAACCCGACGTGCCGGTGTTCTCCCTGACCACTCCTTCTATGCTCCACGACTTCGCGATCACTAAAAAGTACGCGGTGTTCACCGACATTCAGATCGGAATGAACCCGATGGAGATGATCGGCGGCGGATCTCCGGTGGGGTTGGACGCTTCTAAAGTATCTAGAATAGGAATAATTCCGAAATACGCGAAGGACGATTCGGAGATGAGGTGGTTTGAGGTGCCCGGGTTAAACATCATGCACGCGGTTAACGCATGGGACGAAGACGACGCCGTTGTGATGGTGGCGCCGAACATCTTATCGGCGGAGCACACTCTCGAGAGAATGGAGCTCATCCACGCGCTCGTGGAGAAAGTGAGGATCGATCTCAAGACCGGGATCGTTTCGCGCCAACCGATCTCGACCCGGAATCTGGACTTCGCGGTGATAAACCCGGCTTACCAGGGGAAAAAGAACCGGTTCGTGTACGCGGGTGTCGGGGATCCGATGCCGAAGATCTCGGGAGTGGTGAAGCTCGACGTGTCGGGTGATGAGCACAAGGAGAGCATAGTGGCGAGCAGGATGTACGGTCAGGGTTGCTTCGGGGGAGAGCCGTTTTTCGTGGCGAGGGATCCGGAGAATCCGGAGGCGGAGGAGGACGACGGGTACGTGGTGTCGTATGTTCATGATGAGAAGACGGGAGAGTCGAGGTTTTTGGTGATGGACGCCAAGTCGTCTGAGCTGGAAACCGTGGCGGAAGTTAAGCTGCCGCGGCGGGTGCCGTATGGTTTTCACGGACTGTTTGTGAGGGAGGGTGACCTGAAGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
RPE65

Protein Analysis

464

Amino Acids

51.69

Weight (kDa)

8.73

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 101 - 456 4.5e-92 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19170
fragaria_vesca FvH4_4g34690
malus_domestica MD13G1090300.v1.1 MD16G1090700.v1.1
prunus_persica Prupe.1G255500_v2.0.a1 Prupe.1G255500_v2.0.a1
pyrus_communis pycom13g07860 pycom16g07750
rosa_chinensis RchiOBHm_Chr4g0444051
rosa_laevigata RLG00000005862
rosa_multiflora Rmu_co8344017.1_g000001 Rmu_sc0015522.1_g000005
rosa_roxburghii Rroxscaffold_5G00384610
rosa_rugosa Rorug04G0349200
rosa_samantha Rh4AG410000 Rh4BG421100 Rh4CG435500 Rh4DG416400
rosa_wichuraiana Rw4G035220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 422, 1264
AatII GACGTC 1 cut(s) 426
AccB1I GGYRCC 5 cut(s) 269, 535, 802, 868, 1339
AccBSI CCGCTC 1 cut(s) 33
AccIII TCCGGA 2 cut(s) 1195, 1204
AclWI GGATC 8 cut(s) 488, 718, 950, 968, 1058, 1071, 1187, 1200
AcoI YGGCCR 1 cut(s) 411
AcvI CACGTG 1 cut(s) 266
AcyI GRCGYC 6 cut(s) 202, 270, 423, 852, 869, 1287
AfaI GTAC 7 cut(s) 75, 186, 305, 653, 1049, 1148, 1229
AfiI CCNNNNNNNGG 4 cut(s) 317, 438, 503, 994
AflIII ACRYGT 2 cut(s) 263, 1101
AgeI ACCGGT 1 cut(s) 1038
AgsI TTSAA 1 cut(s) 241
AhdI GACNNNNNGTC 1 cut(s) 1291
AjiI CACGTC 2 cut(s) 592, 1102
AjnI CCWGG 1 cut(s) 1023
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 6 cut(s) 342, 460, 913, 1096, 1303, 1327
AluI AGCT 6 cut(s) 342, 460, 913, 1096, 1303, 1327
Alw21I GWGCWC 3 cut(s) 894, 915, 1119
Alw26I GTCTC 3 cut(s) 368, 422, 503
AlwI GGATC 8 cut(s) 488, 718, 950, 968, 1058, 1071, 1187, 1200
Ama87I CYCGRG 4 cut(s) 295, 806, 899, 1081
Aor13HI TCCGGA 2 cut(s) 1195, 1204
AoxI GGCC 7 cut(s) 59, 87, 143, 244, 291, 411, 529
ApaI GGGCCC 1 cut(s) 91
ApeKI GCWGC 3 cut(s) 160, 281, 1327
AsiGI ACCGGT 1 cut(s) 1038
Asp700I GAANNNNTTC 1 cut(s) 1040
AspS9I GGNCC 4 cut(s) 60, 87, 88, 530
AsuC2I CCSGG 6 cut(s) 533, 807, 808, 958, 988, 1016
AsuHPI GGTGA 7 cut(s) 241, 655, 1018, 1102, 1121, 1291, 1388
AvaI CYCGRG 4 cut(s) 295, 806, 899, 1081
BaeGI GKGCMC 2 cut(s) 91, 807
BamHI GGATCC 2 cut(s) 1063, 1192
BanI GGYRCC 5 cut(s) 269, 535, 802, 868, 1339
BanII GRGCYC 2 cut(s) 91, 915
BauI CACGAG 2 cut(s) 337, 926
BbrPI CACGTG 1 cut(s) 266
BbsI GAAGAC 3 cut(s) 220, 852, 1262
Bbv12I GWGCWC 3 cut(s) 894, 915, 1119
BbvCI CCTCAGC 1 cut(s) 256
BbvI GCAGC 3 cut(s) 172, 268, 1314
BccI CCATC 4 cut(s) 135, 685, 856, 1276
BciT130I CCWGG 1 cut(s) 1025
BcnI CCSGG 6 cut(s) 533, 807, 808, 958, 988, 1016
BcoDI GTCTC 3 cut(s) 368, 422, 503
BfaI CTAG 1 cut(s) 744
BfmI CTRYAG 1 cut(s) 158
BfoI RGCGCY 3 cut(s) 46, 273, 872
BglI GCCNNNNNGGC 1 cut(s) 290
BglII AGATCT 1 cut(s) 1077
BisI GCNGC 9 cut(s) 146, 161, 164, 282, 539, 706, 1328, 1331, 1334
BlsI GCNGC 9 cut(s) 147, 162, 165, 283, 540, 707, 1329, 1332, 1335
Bme1390I CCNGG 7 cut(s) 533, 807, 808, 958, 988, 1016, 1025
BmeRI GACNNNNNGTC 1 cut(s) 1291
BmeT110I CYCGRG 4 cut(s) 295, 806, 899, 1081
BmgBI CACGTC 2 cut(s) 592, 1102
BmgT120I GGNCC 4 cut(s) 60, 87, 88, 530
BmiI GGNNCC 8 cut(s) 89, 271, 537, 804, 870, 1065, 1194, 1341
BmrFI CCNGG 7 cut(s) 533, 807, 808, 958, 988, 1016, 1025
BmsI GCATC 2 cut(s) 490, 1059
BpiI GAAGAC 3 cut(s) 220, 852, 1262
Bpu10I CCTNAGC 1 cut(s) 256
BpuEI CTTGAG 1 cut(s) 935
BpuMI CCSGG 6 cut(s) 533, 807, 808, 958, 988, 1016
Bsa29I ATCGAT 2 cut(s) 492, 945
BsaAI YACGTR 2 cut(s) 266, 1231
BsaHI GRCGYC 6 cut(s) 202, 270, 423, 852, 869, 1287
BsaI GGTCTC 2 cut(s) 422, 503
BsaJI CCNNGG 7 cut(s) 140, 294, 432, 806, 1024, 1311, 1331
BsaWI WCCGGW 5 cut(s) 558, 715, 1038, 1195, 1204
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 4 cut(s) 317, 438, 503, 994
Bse118I RCCGGY 4 cut(s) 204, 503, 595, 1038
Bse3DI GCAATG 1 cut(s) 62
BseAI TCCGGA 2 cut(s) 1195, 1204
BseBI CCWGG 1 cut(s) 1025
BseCI ATCGAT 2 cut(s) 492, 945
BseDI CCNNGG 7 cut(s) 140, 294, 432, 806, 1024, 1311, 1331
BseGI GGATG 4 cut(s) 49, 481, 915, 1149
BseLI CCNNNNNNNGG 4 cut(s) 317, 438, 503, 994
BseMI GCAATG 1 cut(s) 62
BseMII CTCAG 3 cut(s) 84, 270, 1290
BseRI GAGGAG 2 cut(s) 206, 1229
BseSI GKGCMC 2 cut(s) 91, 807
BseX3I CGGCCG 1 cut(s) 411
BseXI GCAGC 3 cut(s) 172, 268, 1314
BseYI CCCAGC 1 cut(s) 221
Bsh1285I CGRYCG 2 cut(s) 361, 414
BshFI GGCC 7 cut(s) 61, 89, 145, 246, 293, 413, 531
BshNI GGYRCC 5 cut(s) 269, 535, 802, 868, 1339
BshTI ACCGGT 1 cut(s) 1038
BshVI ATCGAT 2 cut(s) 492, 945
BsiEI CGRYCG 2 cut(s) 361, 414
BsiHKAI GWGCWC 3 cut(s) 894, 915, 1119
BsiHKCI CYCGRG 4 cut(s) 295, 806, 899, 1081
BslFI GGGAC 2 cut(s) 450, 854
BslI CCNNNNNNNGG 4 cut(s) 317, 438, 503, 994
BsmAI GTCTC 3 cut(s) 368, 422, 503
BsmBI CGTCTC 1 cut(s) 368
BsmFI GGGAC 2 cut(s) 450, 854
BsnI GGCC 7 cut(s) 61, 89, 145, 246, 293, 413, 531
Bso31I GGTCTC 2 cut(s) 422, 503
BsoBI CYCGRG 4 cut(s) 295, 806, 899, 1081
Bsp120I GGGCCC 1 cut(s) 87
Bsp1286I GDGCHC 5 cut(s) 91, 807, 894, 915, 1119
Bsp13I TCCGGA 2 cut(s) 1195, 1204
Bsp1407I TGTACA 1 cut(s) 303
Bsp68I TCGCGA 1 cut(s) 638
BspANI GGCC 7 cut(s) 61, 89, 145, 246, 293, 413, 531
BspCNI CTCAG 3 cut(s) 83, 269, 1291
BspDI ATCGAT 2 cut(s) 492, 945
BspEI TCCGGA 2 cut(s) 1195, 1204
BspHI TCATGA 2 cut(s) 465, 1246
BspLI GGNNCC 8 cut(s) 89, 271, 537, 804, 870, 1065, 1194, 1341
BspMAI CTGCAG 1 cut(s) 162
BspPI GGATC 8 cut(s) 488, 718, 950, 968, 1058, 1071, 1187, 1200
BspT107I GGYRCC 5 cut(s) 269, 535, 802, 868, 1339
BspTNI GGTCTC 2 cut(s) 422, 503
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 62
BsrFI RCCGGY 4 cut(s) 204, 503, 595, 1038
BsrGI TGTACA 1 cut(s) 303
BssAI RCCGGY 4 cut(s) 204, 503, 595, 1038
BssECI CCNNGG 7 cut(s) 140, 294, 432, 806, 1024, 1311, 1331
BssNI GRCGYC 6 cut(s) 202, 270, 423, 852, 869, 1287
BssSI CACGAG 2 cut(s) 337, 926
BssT1I CCWWGG 1 cut(s) 140
Bst2BI CACGAG 2 cut(s) 337, 926
Bst2UI CCWGG 1 cut(s) 1025
Bst4CI ACNGT 6 cut(s) 191, 362, 433, 1151, 1312, 1362
Bst6I CTCTTC 1 cut(s) 105
BstACI GRCGYC 6 cut(s) 202, 270, 423, 852, 869, 1287
BstAUI TGTACA 1 cut(s) 303
BstBAI YACGTR 2 cut(s) 266, 1231
BstC8I GCNNGC 8 cut(s) 87, 117, 206, 286, 375, 458, 825, 1138
BstDEI CTNAG 3 cut(s) 70, 256, 1299
BstDSI CCRYGG 3 cut(s) 432, 1311, 1331
BstEII GGTNACC 1 cut(s) 1376
BstF5I GGATG 4 cut(s) 49, 481, 915, 1149
BstH2I RGCGCY 3 cut(s) 46, 273, 872
BstMAI GTCTC 3 cut(s) 368, 422, 503
BstMCI CGRYCG 2 cut(s) 361, 414
BstMWI GCNNNNNNNGC 7 cut(s) 205, 243, 278, 290, 833, 1333, 1339
BstNI CCWGG 1 cut(s) 1025
BstNSI RCATGY 1 cut(s) 119
BstPI GGTNACC 1 cut(s) 1376
BstSCI CCNGG 7 cut(s) 531, 805, 806, 956, 986, 1014, 1023
BstSFI CTRYAG 1 cut(s) 158
BstSLI GKGCMC 2 cut(s) 91, 807
BstV1I GCAGC 3 cut(s) 172, 268, 1314
BstV2I GAAGAC 3 cut(s) 220, 852, 1262
BstX2I RGATCY 4 cut(s) 710, 1063, 1077, 1192
BstYI RGATCY 4 cut(s) 710, 1063, 1077, 1192
BstZI CGGCCG 1 cut(s) 411
Bsu15I ATCGAT 2 cut(s) 492, 945
BsuRI GGCC 7 cut(s) 61, 89, 145, 246, 293, 413, 531
BsuTUI ATCGAT 2 cut(s) 492, 945
BtgI CCRYGG 3 cut(s) 432, 1311, 1331
BtrI CACGTC 2 cut(s) 592, 1102
BtsCI GGATG 4 cut(s) 49, 481, 915, 1149
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 405
BtuMI TCGCGA 1 cut(s) 638
Cac8I GCNNGC 8 cut(s) 87, 117, 206, 286, 375, 458, 825, 1138
CciI TCATGA 2 cut(s) 465, 1246
Cfr10I RCCGGY 4 cut(s) 204, 503, 595, 1038
Cfr13I GGNCC 4 cut(s) 60, 87, 88, 530
Cfr42I CCGCGG 1 cut(s) 1334
Cfr9I CCCGGG 1 cut(s) 806
ClaI ATCGAT 2 cut(s) 492, 945
CseI GACGC 5 cut(s) 210, 214, 737, 860, 1295
Csp6I GTAC 7 cut(s) 74, 185, 304, 652, 1048, 1147, 1228
CspAI ACCGGT 1 cut(s) 1038
CviAII CATG 6 cut(s) 116, 466, 472, 820, 837, 1247
CviQI GTAC 7 cut(s) 74, 185, 304, 652, 1048, 1147, 1228
DdeI CTNAG 3 cut(s) 70, 256, 1299
DinI GGCGCC 2 cut(s) 271, 870
DrdI GACNNNNNNGTC 2 cut(s) 422, 1264
DriI GACNNNNNGTC 1 cut(s) 1291
DseDI GACNNNNNNGTC 2 cut(s) 422, 1264
EaeI YGGCCR 1 cut(s) 411
EagI CGGCCG 1 cut(s) 411
Eam1104I CTCTTC 1 cut(s) 105
Eam1105I GACNNNNNGTC 1 cut(s) 1291
EarI CTCTTC 1 cut(s) 105
EciI GGCGGA 4 cut(s) 723, 902, 1226, 1331
Ecl136II GAGCTC 1 cut(s) 913
EclXI CGGCCG 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 91, 915
Eco31I GGTCTC 2 cut(s) 422, 503
Eco52I CGGCCG 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 913
Eco72I CACGTG 1 cut(s) 266
Eco88I CYCGRG 4 cut(s) 295, 806, 899, 1081
Eco91I GGTNACC 1 cut(s) 1376
EcoICRI GAGCTC 1 cut(s) 913
EcoO109I RGGNCCY 1 cut(s) 88
EcoO65I GGTNACC 1 cut(s) 1376
EcoRII CCWGG 1 cut(s) 1023
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 2 cut(s) 91, 915
EgeI GGCGCC 2 cut(s) 271, 870
EheI GGCGCC 2 cut(s) 271, 870
ErhI CCWWGG 1 cut(s) 140
Esp3I CGTCTC 1 cut(s) 368
FaeI CATG 6 cut(s) 119, 469, 475, 823, 840, 1250
FaqI GGGAC 2 cut(s) 450, 854
FatI CATG 6 cut(s) 115, 465, 471, 819, 836, 1246
FauI CCCGC 6 cut(s) 78, 88, 293, 319, 1045, 1329
Fnu4HI GCNGC 9 cut(s) 146, 161, 164, 282, 539, 706, 1328, 1331, 1334
FokI GGATG 4 cut(s) 36, 468, 902, 1156
FriOI GRGCYC 2 cut(s) 91, 915
Fsp4HI GCNGC 9 cut(s) 146, 161, 164, 282, 539, 706, 1328, 1331, 1334
FspBI CTAG 1 cut(s) 744
GluI GCNGC 9 cut(s) 146, 161, 164, 282, 539, 706, 1328, 1331, 1334
GsaI CCCAGC 1 cut(s) 225
HaeII RGCGCY 3 cut(s) 46, 273, 872
HaeIII GGCC 7 cut(s) 61, 89, 145, 246, 293, 413, 531
HgaI GACGC 5 cut(s) 210, 214, 737, 860, 1295
Hin1I GRCGYC 6 cut(s) 202, 270, 423, 852, 869, 1287
Hin1II CATG 6 cut(s) 119, 469, 475, 823, 840, 1250
HincII GTYRAC 2 cut(s) 408, 832
HindII GTYRAC 2 cut(s) 408, 832
HinfI GANTC 5 cut(s) 133, 779, 991, 1201, 1265
HpaI GTTAAC 1 cut(s) 832
HphI GGTGA 7 cut(s) 241, 655, 1018, 1102, 1121, 1291, 1388
Hpy166II GTNNAC 5 cut(s) 187, 408, 663, 832, 1048
Hpy188I TCNGA 9 cut(s) 132, 486, 549, 675, 680, 762, 784, 1068, 1300
Hpy8I GTNNAC 5 cut(s) 187, 408, 663, 832, 1048
HpyAV CCTTC 3 cut(s) 523, 627, 766
HpyCH4III ACNGT 6 cut(s) 191, 362, 433, 1151, 1312, 1362
HpyCH4IV ACGT 6 cut(s) 169, 265, 423, 591, 1101, 1230
HpyCH4V TGCA 2 cut(s) 160, 823
HpyF10VI GCNNNNNNNGC 7 cut(s) 205, 243, 278, 290, 833, 1333, 1339
HpyF3I CTNAG 3 cut(s) 70, 256, 1299
HpySE526I ACGT 6 cut(s) 169, 265, 423, 591, 1101, 1230
Hsp92I GRCGYC 6 cut(s) 202, 270, 423, 852, 869, 1287
Hsp92II CATG 6 cut(s) 119, 469, 475, 823, 840, 1250
KasI GGCGCC 2 cut(s) 269, 868
Kpn2I TCCGGA 2 cut(s) 1195, 1204
KroI GCCGGC 1 cut(s) 204
KroNI GCCGGC 1 cut(s) 206
KspAI GTTAAC 1 cut(s) 832
KspI CCGCGG 1 cut(s) 1334
LmnI GCTCC 5 cut(s) 124, 575, 630, 889, 910
Lsp1109I GCAGC 3 cut(s) 172, 268, 1314
LweI GCATC 2 cut(s) 490, 1059
MaeI CTAG 1 cut(s) 744
MaeII ACGT 6 cut(s) 169, 265, 423, 591, 1101, 1230
MaeIII GTNAC 2 cut(s) 524, 1376
MbiI CCGCTC 1 cut(s) 33
MboII GAAGA 7 cut(s) 92, 220, 380, 499, 857, 1087, 1267
MflI RGATCY 4 cut(s) 710, 1063, 1077, 1192
MhlI GDGCHC 5 cut(s) 91, 807, 894, 915, 1119
MluCI AATT 1 cut(s) 756
Mly113I GGCGCC 2 cut(s) 270, 869
MlyI GAGTC 1 cut(s) 1274
MmeI TCCRAC 1 cut(s) 705
MroI TCCGGA 2 cut(s) 1195, 1204
MroNI GCCGGC 1 cut(s) 204
MroXI GAANNNNTTC 1 cut(s) 1040
MseI TTAA 4 cut(s) 450, 812, 831, 1323
MslI CAYNNNNRTG 1 cut(s) 470
MspA1I CMGCKG 2 cut(s) 281, 1333
MspR9I CCNGG 7 cut(s) 533, 807, 808, 958, 988, 1016, 1025
MvaI CCWGG 1 cut(s) 1025
MwoI GCNNNNNNNGC 7 cut(s) 205, 243, 278, 290, 833, 1333, 1339
NaeI GCCGGC 1 cut(s) 206
NarI GGCGCC 2 cut(s) 270, 869
NciI CCSGG 6 cut(s) 533, 807, 808, 958, 988, 1016
NgoMIV GCCGGC 1 cut(s) 204
NlaIII CATG 6 cut(s) 119, 469, 475, 823, 840, 1250
NlaIV GGNNCC 8 cut(s) 89, 271, 537, 804, 870, 1065, 1194, 1341
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 1 cut(s) 1376
NruI TCGCGA 1 cut(s) 638
NspI RCATGY 1 cut(s) 119
PaeI GCATGC 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 899
PagI TCATGA 2 cut(s) 465, 1246
PdiI GCCGGC 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 1040
PfeI GAWTC 4 cut(s) 133, 779, 991, 1201
PinAI ACCGGT 1 cut(s) 1038
PkrI GCNGC 9 cut(s) 147, 162, 165, 283, 540, 707, 1329, 1332, 1335
PleI GAGTC 1 cut(s) 1273
PluTI GGCGCC 2 cut(s) 273, 872
PmaCI CACGTG 1 cut(s) 266
PmlI CACGTG 1 cut(s) 266
PpsI GAGTC 1 cut(s) 1273
Ppu21I YACGTR 2 cut(s) 266, 1231
Psp124BI GAGCTC 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 1023
PspCI CACGTG 1 cut(s) 266
PspEI GGTNACC 1 cut(s) 1376
PspFI CCCAGC 1 cut(s) 221
PspGI CCWGG 1 cut(s) 1023
PspN4I GGNNCC 8 cut(s) 89, 271, 537, 804, 870, 1065, 1194, 1341
PspOMI GGGCCC 1 cut(s) 87
PspPI GGNCC 4 cut(s) 60, 87, 88, 530
PsrI GAACNNNNNNTAC 4 cut(s) 57, 89, 644, 676
PstI CTGCAG 1 cut(s) 162
PsuI RGATCY 4 cut(s) 710, 1063, 1077, 1192
RruI TCGCGA 1 cut(s) 638
RsaI GTAC 7 cut(s) 75, 186, 305, 653, 1049, 1148, 1229
RsaNI GTAC 7 cut(s) 74, 185, 304, 652, 1048, 1147, 1228
RseI CAYNNNNRTG 1 cut(s) 470
SacI GAGCTC 1 cut(s) 915
SacII CCGCGG 1 cut(s) 1334
SaqAI TTAA 4 cut(s) 450, 812, 831, 1323
SatI GCNGC 9 cut(s) 146, 161, 164, 282, 539, 706, 1328, 1331, 1334
Sau96I GGNCC 4 cut(s) 60, 87, 88, 530
SchI GAGTC 1 cut(s) 1274
ScrFI CCNGG 7 cut(s) 533, 807, 808, 958, 988, 1016, 1025
SduI GDGCHC 5 cut(s) 91, 807, 894, 915, 1119
SfaNI GCATC 2 cut(s) 490, 1059
SfcI CTRYAG 1 cut(s) 158
SfoI GGCGCC 2 cut(s) 271, 870
Sfr274I CTCGAG 1 cut(s) 899
Sfr303I CCGCGG 1 cut(s) 1334
SgrAI CRCCGGYG 1 cut(s) 503
SgrBI CCGCGG 1 cut(s) 1334
SlaI CTCGAG 1 cut(s) 899
SmaI CCCGGG 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 2 cut(s) 899, 950
SmoI CTYRAG 2 cut(s) 899, 950
SphI GCATGC 1 cut(s) 119
Sse9I AATT 1 cut(s) 756
SspDI GGCGCC 2 cut(s) 269, 868
SspMI CTAG 1 cut(s) 744
SstI GAGCTC 1 cut(s) 915
StyD4I CCNGG 7 cut(s) 531, 805, 806, 956, 986, 1014, 1023
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 6 cut(s) 191, 362, 433, 1151, 1312, 1362
TaiI ACGT 6 cut(s) 172, 268, 426, 594, 1104, 1233
TasI AATT 1 cut(s) 756
TatI WGTACW 2 cut(s) 184, 303
TauI GCSGC 6 cut(s) 148, 166, 541, 708, 1333, 1336
TfiI GAWTC 4 cut(s) 133, 779, 991, 1201
Tru1I TTAA 4 cut(s) 450, 812, 831, 1323
Tru9I TTAA 4 cut(s) 450, 812, 831, 1323
TscAI CASTG 1 cut(s) 405
TseFI GTSAC 1 cut(s) 1376
TseI GCWGC 3 cut(s) 160, 281, 1327
Tsp45I GTSAC 1 cut(s) 1376
TspDTI ATGAA 3 cut(s) 454, 698, 1235
TspGWI ACGGA 3 cut(s) 454, 512, 1371
TspMI CCCGGG 1 cut(s) 806
TspRI CASTG 1 cut(s) 405
XbaI TCTAGA 1 cut(s) 743
XceI RCATGY 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 899
XmaI CCCGGG 1 cut(s) 806
XmnI GAANNNNTTC 1 cut(s) 1040
XspI CTAG 1 cut(s) 744
ZraI GACGTC 1 cut(s) 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.