Rw4G035220

carotenoid cleavage dioxygenase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012126
Physical Location & Seq
Forward (+)
62024799 .. 62026559
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G035220.1

Sequence Viewer

Length: 1761 bp
ATGGATGCCTTGTCTTCTTCTTTCCTCTCCACATTGCCCACTCGATCTTCAACAACAGCAGCACCAAAACTCACCCCAACAAAATCCCTCAACATCTCCTCTGTTAGAATTGAAGAAAGACCCACAACCCAAACACCTCCAAAAACCACAACACCACAACCCCCGCCAAAACAGACTCCTCCTCCTCCATCCTACTCCTCACCACCACCCACAAAACCTAATGCACCAGCACTTCCCGCTGTGATCTTCAACCTCTTCGACGACTTCATCAACAACTTCGTCGACCCGCCGGTCCGGTCTTCAGTCGACCCCAAACAGGCCCTCTCCGGTAACTTCGCTCCCCTCACCGAACTTCCCCCAACCGAATGTCAAGTAATCAAAGGCTCATTACCCTCCGCTCTCGACGGCGCTTACATCCGCAATGGCCCCAACCCTCAGTACCTGCCGCGCGGGCCCTACCACCTCTTCGACGGCGACGGCATGCTCCACTCCATCAGAATCTCCCAAGGCCGCGCCGTCCTCTGCAGCCGCTTCGTCAAGACCTACAAGTTCACCGTCGAGCGCGACGCCGGCTTCCCTCTCCTCCCCAACGTCTTCTCCGGCTTCAACGGCCTCACCGCCTCCGCGACACGTGGCGCCCTCTCCGCTGCCCGCATGGCCTCGGGGCTGTTCAATCCCGCCAACGGCATTGGCAACGCGAACACCAGCTTGGCGTTTTTCGGCGACCGTCTCTACGCGCTCGCCGAGTCTGATCTTCCTTACGCCGTGCGGCTGACGGCCGACGGAGACGTCGAGACCGTGGGACGGAACGACTTTAACGGCGAGCTGTTCATGAGCATGACGGCGCATCCGAAGATCGATCCAGACACCGGCGAGACCTTCGCTTTCCGTTACGGCCCGGTGCCGCCGTTTCTCACTTACTTCCGGTTCGACCCTACCGGAGCGAAACAAGCCGACGTGCCGGTGTTCTCCCTGACCAGTCCTTCTATGCTCCACGACTTCGCGATCACTAAAAAGTACGCGGTGTTCACCGACATTCAGATCGGAATGAACCCGATGGAGATGATCGGCGGAGGATCTCCGGTGGGGTTGGACGCTTCTAAAGTATCTAGAATAGGAATAATTCCGAAATACGCGAAGGACGATTCCGAGATGAGGTGGTTTGAGGTGCCCGGGTTCAACATCATGCACGCGGTTAACGCATGGGACGAAGACGACGCCGTTGTGATGGTGGCGCCGAACCTCTTATCGGCGGAGCACACTCTCGAGAGAATGGAGCTCGTCCACGCGCTCGTGGAGAAGGTGAGGATCGATCTCAAGACCGGGATCGTTTCGCGCCAACCGATCTCGACCCGGAATCTGGACTTCGCGGTGATAAACCCGGCTTACCAGGGGAAAAAGAACCGGTTCGTGTACGCGGGCGTCGGGGATCCGATGCCGAAGATATCGGGAGTGGTGAAGCTCGACGTGTCGGGTGATGAGCACAAGGAGAGCATAGTGGCGAGCAGGATGTACGGTCAGGGTTGCTTCGGGGGAGAGCCGTTTTTCGTGGCGAGGGATCCGGAGAATCCGGAGGCGGAGGAGGACGACGGGTACGTGGTGTCGTATGTTCATGATGAGAAGACGGGAGAGTCGAGGTTTTTGGTGATGGACGCCAAGTCGTCTGCGCTGGAAACCGTGGCGGAAGTTAAGCTGCCGCGGCGGGTGCCTTACGGTTTTCACGGACTGTTTGTGAGGGAGAGTGACCTGAAGAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00906 ko01100 ko01110 map00906 map01100 map01110
Pfam Domains
Protein Families
RPE65

Protein Analysis

586

Amino Acids

63.86

Weight (kDa)

5.78

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 115 - 578 1.1e-122 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19170
fragaria_vesca FvH4_4g34690
malus_domestica MD13G1090300.v1.1 MD16G1090700.v1.1
prunus_persica Prupe.1G255500_v2.0.a1 Prupe.1G255500_v2.0.a1
pyrus_communis pycom13g07860 pycom16g07750
rosa_chinensis RchiOBHm_Chr4g0444051
rosa_laevigata RLG00000005862
rosa_multiflora Rmu_co8344017.1_g000001 Rmu_sc0015522.1_g000005
rosa_roxburghii Rroxscaffold_5G00384610
rosa_rugosa Rorug04G0349200
rosa_samantha Rh4AG410000 Rh4BG421100 Rh4CG435500 Rh4DG416400
rosa_wichuraiana Rw4G035220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 290, 788, 1630
AatII GACGTC 1 cut(s) 792
Acc36I ACCTGC 1 cut(s) 450
AccB1I GGYRCC 5 cut(s) 635, 901, 1168, 1234, 1705
AccBSI CCGCTC 1 cut(s) 398
AccI GTMKAC 2 cut(s) 282, 306
AccIII TCCGGA 2 cut(s) 1561, 1570
AclWI GGATC 8 cut(s) 854, 1084, 1316, 1334, 1424, 1437, 1553, 1566
AcoI YGGCCR 1 cut(s) 777
AcuI CTGAAG 1 cut(s) 285
AcvI CACGTG 1 cut(s) 632
AcyI GRCGYC 7 cut(s) 567, 636, 789, 1218, 1235, 1422, 1653
AfaI GTAC 5 cut(s) 440, 1019, 1415, 1514, 1595
AfiI CCNNNNNNNGG 7 cut(s) 316, 683, 804, 869, 1155, 1249, 1360
AflIII ACRYGT 2 cut(s) 629, 1467
AgeI ACCGGT 1 cut(s) 1404
AgsI TTSAA 6 cut(s) 51, 113, 250, 607, 673, 1180
AhdI GACNNNNNGTC 1 cut(s) 1657
AjiI CACGTC 2 cut(s) 958, 1468
AjnI CCWGG 1 cut(s) 1389
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
AluBI AGCT 5 cut(s) 708, 826, 1279, 1462, 1693
AluI AGCT 5 cut(s) 708, 826, 1279, 1462, 1693
Alw21I GWGCWC 3 cut(s) 1260, 1281, 1485
Alw26I GTCTC 4 cut(s) 734, 780, 788, 869
AlwI GGATC 8 cut(s) 854, 1084, 1316, 1334, 1424, 1437, 1553, 1566
AlwNI CAGNNNCTG 1 cut(s) 442
Ama87I CYCGRG 3 cut(s) 661, 1172, 1265
Aor13HI TCCGGA 2 cut(s) 1561, 1570
AoxI GGCC 8 cut(s) 318, 424, 452, 508, 610, 657, 777, 895
ApaI GGGCCC 1 cut(s) 456
ApeKI GCWGC 4 cut(s) 59, 525, 647, 1693
AsiGI ACCGGT 1 cut(s) 1404
Asp700I GAANNNNTTC 1 cut(s) 1406
AspS9I GGNCC 6 cut(s) 292, 319, 425, 452, 453, 896
AsuC2I CCSGG 6 cut(s) 899, 1173, 1174, 1324, 1354, 1382
AvaI CYCGRG 3 cut(s) 661, 1172, 1265
AvaII GGWCC 1 cut(s) 292
BaeGI GKGCMC 2 cut(s) 456, 1173
BamHI GGATCC 2 cut(s) 1429, 1558
BanI GGYRCC 5 cut(s) 635, 901, 1168, 1234, 1705
BanII GRGCYC 2 cut(s) 456, 1281
BauI CACGAG 1 cut(s) 1292
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 5 cut(s) 6, 291, 586, 1218, 1628
Bbv12I GWGCWC 3 cut(s) 1260, 1281, 1485
BbvI GCAGC 4 cut(s) 71, 537, 634, 1680
BccI CCATC 5 cut(s) 196, 500, 1051, 1222, 1642
BcgI CGANNNNNNTGC 2 cut(s) 514, 548
BciT130I CCWGG 1 cut(s) 1391
BcnI CCSGG 6 cut(s) 899, 1173, 1174, 1324, 1354, 1382
BcoDI GTCTC 4 cut(s) 734, 780, 788, 869
BfaI CTAG 1 cut(s) 1110
BfmI CTRYAG 1 cut(s) 523
BfoI RGCGCY 3 cut(s) 411, 639, 1238
BfuAI ACCTGC 1 cut(s) 450
BglI GCCNNNNNGGC 2 cut(s) 451, 656
Bme1390I CCNGG 7 cut(s) 899, 1173, 1174, 1324, 1354, 1382, 1391
Bme18I GGWCC 1 cut(s) 292
BmeRI GACNNNNNGTC 1 cut(s) 1657
BmeT110I CYCGRG 3 cut(s) 661, 1172, 1265
BmgBI CACGTC 2 cut(s) 958, 1468
BmgT120I GGNCC 6 cut(s) 292, 319, 425, 452, 453, 896
BmiI GGNNCC 9 cut(s) 427, 454, 637, 903, 1170, 1236, 1431, 1560, 1707
BmrFI CCNGG 7 cut(s) 899, 1173, 1174, 1324, 1354, 1382, 1391
BmsI GCATC 2 cut(s) 856, 1425
BpiI GAAGAC 5 cut(s) 6, 291, 586, 1218, 1628
BpuEI CTTGAG 1 cut(s) 1301
BpuMI CCSGG 6 cut(s) 899, 1173, 1174, 1324, 1354, 1382
Bsa29I ATCGAT 2 cut(s) 858, 1311
BsaAI YACGTR 2 cut(s) 632, 1597
BsaHI GRCGYC 7 cut(s) 567, 636, 789, 1218, 1235, 1422, 1653
BsaI GGTCTC 2 cut(s) 788, 869
BsaJI CCNNGG 7 cut(s) 505, 660, 798, 1172, 1390, 1677, 1697
BsaWI WCCGGW 8 cut(s) 294, 326, 924, 938, 1081, 1404, 1561, 1570
BsaXI ACNNNNNCTCC 4 cut(s) 166, 196, 581, 611
Bsc4I CCNNNNNNNGG 7 cut(s) 316, 683, 804, 869, 1155, 1249, 1360
Bse118I RCCGGY 5 cut(s) 289, 569, 869, 961, 1404
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 2 cut(s) 32, 427
BseAI TCCGGA 2 cut(s) 1561, 1570
BseBI CCWGG 1 cut(s) 1391
BseCI ATCGAT 2 cut(s) 858, 1311
BseDI CCNNGG 7 cut(s) 505, 660, 798, 1172, 1390, 1677, 1697
BseGI GGATG 5 cut(s) 10, 188, 414, 847, 1515
BseLI CCNNNNNNNGG 7 cut(s) 316, 683, 804, 869, 1155, 1249, 1360
BseMI GCAATG 2 cut(s) 32, 427
BseMII CTCAG 1 cut(s) 449
BseNI ACTGG 1 cut(s) 978
BseRI GAGGAG 7 cut(s) 88, 168, 171, 174, 187, 572, 1595
BseSI GKGCMC 2 cut(s) 456, 1173
BseX3I CGGCCG 1 cut(s) 777
BseXI GCAGC 4 cut(s) 71, 537, 634, 1680
Bsh1285I CGRYCG 2 cut(s) 727, 780
BshFI GGCC 8 cut(s) 320, 426, 454, 510, 612, 659, 779, 897
BshNI GGYRCC 5 cut(s) 635, 901, 1168, 1234, 1705
BshTI ACCGGT 1 cut(s) 1404
BshVI ATCGAT 2 cut(s) 858, 1311
BsiEI CGRYCG 2 cut(s) 727, 780
BsiHKAI GWGCWC 3 cut(s) 1260, 1281, 1485
BsiHKCI CYCGRG 3 cut(s) 661, 1172, 1265
BslFI GGGAC 2 cut(s) 816, 1220
BslI CCNNNNNNNGG 7 cut(s) 316, 683, 804, 869, 1155, 1249, 1360
BsmAI GTCTC 4 cut(s) 734, 780, 788, 869
BsmBI CGTCTC 2 cut(s) 734, 780
BsmFI GGGAC 2 cut(s) 816, 1220
BsnI GGCC 8 cut(s) 320, 426, 454, 510, 612, 659, 779, 897
Bso31I GGTCTC 2 cut(s) 788, 869
BsoBI CYCGRG 3 cut(s) 661, 1172, 1265
Bsp120I GGGCCC 1 cut(s) 452
Bsp1286I GDGCHC 5 cut(s) 456, 1173, 1260, 1281, 1485
Bsp13I TCCGGA 2 cut(s) 1561, 1570
Bsp68I TCGCGA 1 cut(s) 1004
BspANI GGCC 8 cut(s) 320, 426, 454, 510, 612, 659, 779, 897
BspCNI CTCAG 1 cut(s) 448
BspDI ATCGAT 2 cut(s) 858, 1311
BspEI TCCGGA 2 cut(s) 1561, 1570
BspHI TCATGA 2 cut(s) 831, 1612
BspLI GGNNCC 9 cut(s) 427, 454, 637, 903, 1170, 1236, 1431, 1560, 1707
BspMAI CTGCAG 1 cut(s) 527
BspMI ACCTGC 1 cut(s) 450
BspPI GGATC 8 cut(s) 854, 1084, 1316, 1334, 1424, 1437, 1553, 1566
BspT107I GGYRCC 5 cut(s) 635, 901, 1168, 1234, 1705
BspTNI GGTCTC 2 cut(s) 788, 869
BsrBI CCGCTC 1 cut(s) 398
BsrDI GCAATG 2 cut(s) 32, 427
BsrFI RCCGGY 5 cut(s) 289, 569, 869, 961, 1404
BsrI ACTGG 1 cut(s) 978
BssAI RCCGGY 5 cut(s) 289, 569, 869, 961, 1404
BssECI CCNNGG 7 cut(s) 505, 660, 798, 1172, 1390, 1677, 1697
BssNI GRCGYC 7 cut(s) 567, 636, 789, 1218, 1235, 1422, 1653
BssSI CACGAG 1 cut(s) 1292
BssT1I CCWWGG 1 cut(s) 505
Bst2BI CACGAG 1 cut(s) 1292
Bst2UI CCWGG 1 cut(s) 1391
Bst4CI ACNGT 7 cut(s) 556, 728, 799, 1517, 1678, 1715, 1728
Bst6I CTCTTC 2 cut(s) 260, 470
BstACI GRCGYC 7 cut(s) 567, 636, 789, 1218, 1235, 1422, 1653
BstBAI YACGTR 2 cut(s) 632, 1597
BstC8I GCNNGC 9 cut(s) 452, 482, 571, 652, 741, 824, 1191, 1420, 1504
BstDEI CTNAG 1 cut(s) 435
BstDSI CCRYGG 3 cut(s) 798, 1677, 1697
BstF5I GGATG 5 cut(s) 10, 188, 414, 847, 1515
BstH2I RGCGCY 3 cut(s) 411, 639, 1238
BstMAI GTCTC 4 cut(s) 734, 780, 788, 869
BstMCI CGRYCG 2 cut(s) 727, 780
BstNI CCWGG 1 cut(s) 1391
BstNSI RCATGY 1 cut(s) 484
BstSCI CCNGG 7 cut(s) 897, 1171, 1172, 1322, 1352, 1380, 1389
BstSFI CTRYAG 1 cut(s) 523
BstSLI GKGCMC 2 cut(s) 456, 1173
BstV1I GCAGC 4 cut(s) 71, 537, 634, 1680
BstV2I GAAGAC 5 cut(s) 6, 291, 586, 1218, 1628
BstX2I RGATCY 3 cut(s) 1076, 1429, 1558
BstYI RGATCY 3 cut(s) 1076, 1429, 1558
BstZI CGGCCG 1 cut(s) 777
Bsu15I ATCGAT 2 cut(s) 858, 1311
BsuRI GGCC 8 cut(s) 320, 426, 454, 510, 612, 659, 779, 897
BsuTUI ATCGAT 2 cut(s) 858, 1311
BtgI CCRYGG 3 cut(s) 798, 1677, 1697
BtrI CACGTC 2 cut(s) 958, 1468
BtsCI GGATG 5 cut(s) 10, 188, 414, 847, 1515
BtuMI TCGCGA 1 cut(s) 1004
BveI ACCTGC 1 cut(s) 450
Cac8I GCNNGC 9 cut(s) 452, 482, 571, 652, 741, 824, 1191, 1420, 1504
CaiI CAGNNNCTG 1 cut(s) 442
CciI TCATGA 2 cut(s) 831, 1612
Cfr10I RCCGGY 5 cut(s) 289, 569, 869, 961, 1404
Cfr13I GGNCC 6 cut(s) 292, 319, 425, 452, 453, 896
Cfr42I CCGCGG 1 cut(s) 1700
Cfr9I CCCGGG 1 cut(s) 1172
ClaI ATCGAT 2 cut(s) 858, 1311
CpoI CGGWCCG 1 cut(s) 292
CseI GACGC 5 cut(s) 575, 1103, 1226, 1411, 1661
Csp6I GTAC 5 cut(s) 439, 1018, 1414, 1513, 1594
CspAI ACCGGT 1 cut(s) 1404
CspI CGGWCCG 1 cut(s) 292
CviAII CATG 7 cut(s) 481, 655, 832, 838, 1186, 1203, 1613
CviQI GTAC 5 cut(s) 439, 1018, 1414, 1513, 1594
DdeI CTNAG 1 cut(s) 435
DinI GGCGCC 2 cut(s) 637, 1236
DrdI GACNNNNNNGTC 3 cut(s) 290, 788, 1630
DriI GACNNNNNGTC 1 cut(s) 1657
DseDI GACNNNNNNGTC 3 cut(s) 290, 788, 1630
EaeI YGGCCR 1 cut(s) 777
EagI CGGCCG 1 cut(s) 777
Eam1104I CTCTTC 2 cut(s) 260, 470
Eam1105I GACNNNNNGTC 1 cut(s) 1657
EarI CTCTTC 2 cut(s) 260, 470
EciI GGCGGA 4 cut(s) 1086, 1268, 1592, 1697
Ecl136II GAGCTC 1 cut(s) 1279
EclXI CGGCCG 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 505
Eco24I GRGCYC 2 cut(s) 456, 1281
Eco31I GGTCTC 2 cut(s) 788, 869
Eco32I GATATC 1 cut(s) 1446
Eco47I GGWCC 1 cut(s) 292
Eco52I CGGCCG 1 cut(s) 777
Eco53kI GAGCTC 1 cut(s) 1279
Eco57I CTGAAG 1 cut(s) 285
Eco72I CACGTG 1 cut(s) 632
Eco88I CYCGRG 3 cut(s) 661, 1172, 1265
EcoICRI GAGCTC 1 cut(s) 1279
EcoO109I RGGNCCY 2 cut(s) 319, 453
EcoRII CCWGG 1 cut(s) 1389
EcoRV GATATC 1 cut(s) 1446
EcoT14I CCWWGG 1 cut(s) 505
EcoT38I GRGCYC 2 cut(s) 456, 1281
EgeI GGCGCC 2 cut(s) 637, 1236
EheI GGCGCC 2 cut(s) 637, 1236
ErhI CCWWGG 1 cut(s) 505
Esp3I CGTCTC 2 cut(s) 734, 780
FaeI CATG 7 cut(s) 484, 658, 835, 841, 1189, 1206, 1616
FaqI GGGAC 2 cut(s) 816, 1220
FatI CATG 7 cut(s) 480, 654, 831, 837, 1185, 1202, 1612
FauI CCCGC 8 cut(s) 171, 244, 294, 443, 659, 685, 1411, 1695
FblI GTMKAC 2 cut(s) 282, 306
FokI GGATG 5 cut(s) 17, 175, 401, 834, 1522
FriOI GRGCYC 2 cut(s) 456, 1281
FspBI CTAG 1 cut(s) 1110
HaeII RGCGCY 3 cut(s) 411, 639, 1238
HaeIII GGCC 8 cut(s) 320, 426, 454, 510, 612, 659, 779, 897
HgaI GACGC 5 cut(s) 575, 1103, 1226, 1411, 1661
Hin1I GRCGYC 7 cut(s) 567, 636, 789, 1218, 1235, 1422, 1653
Hin1II CATG 7 cut(s) 484, 658, 835, 841, 1189, 1206, 1616
HincII GTYRAC 3 cut(s) 283, 307, 1198
HindII GTYRAC 3 cut(s) 283, 307, 1198
HinfI GANTC 7 cut(s) 175, 498, 746, 1145, 1357, 1567, 1631
HpaI GTTAAC 1 cut(s) 1198
Hpy166II GTNNAC 7 cut(s) 283, 307, 552, 1029, 1198, 1285, 1414
Hpy188I TCNGA 8 cut(s) 497, 751, 852, 1041, 1046, 1128, 1150, 1434
Hpy8I GTNNAC 7 cut(s) 283, 307, 552, 1029, 1198, 1285, 1414
HpyAV CCTTC 4 cut(s) 889, 993, 1132, 1294
HpyCH4III ACNGT 7 cut(s) 556, 728, 799, 1517, 1678, 1715, 1728
HpyCH4IV ACGT 6 cut(s) 591, 631, 789, 957, 1467, 1596
HpyCH4V TGCA 3 cut(s) 224, 525, 1189
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 6 cut(s) 591, 631, 789, 957, 1467, 1596
Hsp92I GRCGYC 7 cut(s) 567, 636, 789, 1218, 1235, 1422, 1653
Hsp92II CATG 7 cut(s) 484, 658, 835, 841, 1189, 1206, 1616
KasI GGCGCC 2 cut(s) 635, 1234
Kpn2I TCCGGA 2 cut(s) 1561, 1570
KroI GCCGGC 1 cut(s) 569
KroNI GCCGGC 1 cut(s) 571
KspAI GTTAAC 1 cut(s) 1198
KspI CCGCGG 1 cut(s) 1700
LmnI GCTCC 6 cut(s) 343, 489, 941, 996, 1255, 1276
Lsp1109I GCAGC 4 cut(s) 71, 537, 634, 1680
LweI GCATC 2 cut(s) 856, 1425
MaeI CTAG 1 cut(s) 1110
MaeII ACGT 6 cut(s) 591, 631, 789, 957, 1467, 1596
MaeIII GTNAC 3 cut(s) 329, 890, 1742
MbiI CCGCTC 1 cut(s) 398
MflI RGATCY 3 cut(s) 1076, 1429, 1558
MhlI GDGCHC 5 cut(s) 456, 1173, 1260, 1281, 1485
MluCI AATT 2 cut(s) 108, 1122
Mly113I GGCGCC 2 cut(s) 636, 1235
MlyI GAGTC 3 cut(s) 169, 755, 1640
MmeI TCCRAC 1 cut(s) 1071
MroI TCCGGA 2 cut(s) 1561, 1570
MroNI GCCGGC 1 cut(s) 569
MroXI GAANNNNTTC 1 cut(s) 1406
MseI TTAA 3 cut(s) 816, 1197, 1689
MslI CAYNNNNRTG 1 cut(s) 836
MspA1I CMGCKG 3 cut(s) 239, 647, 1699
MspR9I CCNGG 7 cut(s) 899, 1173, 1174, 1324, 1354, 1382, 1391
MvaI CCWGG 1 cut(s) 1391
NaeI GCCGGC 1 cut(s) 571
NarI GGCGCC 2 cut(s) 636, 1235
NciI CCSGG 6 cut(s) 899, 1173, 1174, 1324, 1354, 1382
NgoMIV GCCGGC 1 cut(s) 569
NlaIII CATG 7 cut(s) 484, 658, 835, 841, 1189, 1206, 1616
NlaIV GGNNCC 9 cut(s) 427, 454, 637, 903, 1170, 1236, 1431, 1560, 1707
NmeAIII GCCGAG 1 cut(s) 769
NmuCI GTSAC 1 cut(s) 1742
NruI TCGCGA 1 cut(s) 1004
NspI RCATGY 1 cut(s) 484
PaeI GCATGC 1 cut(s) 484
PaeR7I CTCGAG 1 cut(s) 1265
PagI TCATGA 2 cut(s) 831, 1612
PcsI WCGNNNNNNNCGW 9 cut(s) 741, 789, 795, 848, 1140, 1206, 1340, 1593, 1631
PdiI GCCGGC 1 cut(s) 571
PdmI GAANNNNTTC 1 cut(s) 1406
PfeI GAWTC 4 cut(s) 498, 1145, 1357, 1567
PinAI ACCGGT 1 cut(s) 1404
PleI GAGTC 3 cut(s) 169, 754, 1639
PluTI GGCGCC 2 cut(s) 639, 1238
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 3 cut(s) 169, 754, 1639
Ppu21I YACGTR 2 cut(s) 632, 1597
Psp124BI GAGCTC 1 cut(s) 1281
Psp6I CCWGG 1 cut(s) 1389
PspCI CACGTG 1 cut(s) 632
PspGI CCWGG 1 cut(s) 1389
PspN4I GGNNCC 9 cut(s) 427, 454, 637, 903, 1170, 1236, 1431, 1560, 1707
PspOMI GGGCCC 1 cut(s) 452
PspPI GGNCC 6 cut(s) 292, 319, 425, 452, 453, 896
PsrI GAACNNNNNNTAC 2 cut(s) 1010, 1042
PstI CTGCAG 1 cut(s) 527
PstNI CAGNNNCTG 1 cut(s) 442
PsuI RGATCY 3 cut(s) 1076, 1429, 1558
RruI TCGCGA 1 cut(s) 1004
RsaI GTAC 5 cut(s) 440, 1019, 1415, 1514, 1595
RsaNI GTAC 5 cut(s) 439, 1018, 1414, 1513, 1594
RseI CAYNNNNRTG 1 cut(s) 836
Rsr2I CGGWCCG 1 cut(s) 292
RsrII CGGWCCG 1 cut(s) 292
SacI GAGCTC 1 cut(s) 1281
SacII CCGCGG 1 cut(s) 1700
SalI GTCGAC 2 cut(s) 281, 305
SaqAI TTAA 3 cut(s) 816, 1197, 1689
Sau96I GGNCC 6 cut(s) 292, 319, 425, 452, 453, 896
SchI GAGTC 3 cut(s) 169, 755, 1640
ScrFI CCNGG 7 cut(s) 899, 1173, 1174, 1324, 1354, 1382, 1391
SduI GDGCHC 5 cut(s) 456, 1173, 1260, 1281, 1485
SfaNI GCATC 2 cut(s) 856, 1425
SfcI CTRYAG 1 cut(s) 523
SfoI GGCGCC 2 cut(s) 637, 1236
Sfr274I CTCGAG 1 cut(s) 1265
Sfr303I CCGCGG 1 cut(s) 1700
SgrAI CRCCGGYG 1 cut(s) 869
SgrBI CCGCGG 1 cut(s) 1700
SinI GGWCC 1 cut(s) 292
SlaI CTCGAG 1 cut(s) 1265
SmaI CCCGGG 1 cut(s) 1174
SmiMI CAYNNNNRTG 1 cut(s) 836
SmlI CTYRAG 2 cut(s) 1265, 1316
SmoI CTYRAG 2 cut(s) 1265, 1316
SphI GCATGC 1 cut(s) 484
Sse9I AATT 2 cut(s) 108, 1122
SspDI GGCGCC 2 cut(s) 635, 1234
SspMI CTAG 1 cut(s) 1110
SstI GAGCTC 1 cut(s) 1281
StyD4I CCNGG 7 cut(s) 897, 1171, 1172, 1322, 1352, 1380, 1389
StyI CCWWGG 1 cut(s) 505
TaaI ACNGT 7 cut(s) 556, 728, 799, 1517, 1678, 1715, 1728
TaiI ACGT 6 cut(s) 594, 634, 792, 960, 1470, 1599
TasI AATT 2 cut(s) 108, 1122
TauI GCSGC 7 cut(s) 448, 513, 531, 772, 907, 1699, 1702
TfiI GAWTC 4 cut(s) 498, 1145, 1357, 1567
Tru1I TTAA 3 cut(s) 816, 1197, 1689
Tru9I TTAA 3 cut(s) 816, 1197, 1689
TseFI GTSAC 1 cut(s) 1742
TseI GCWGC 4 cut(s) 59, 525, 647, 1693
Tsp45I GTSAC 1 cut(s) 1742
TspDTI ATGAA 4 cut(s) 256, 820, 1064, 1601
TspGWI ACGGA 4 cut(s) 798, 820, 878, 1737
TspMI CCCGGG 1 cut(s) 1172
VpaK11BI GGWCC 1 cut(s) 292
XbaI TCTAGA 1 cut(s) 1109
XceI RCATGY 1 cut(s) 484
XhoI CTCGAG 1 cut(s) 1265
XmaI CCCGGG 1 cut(s) 1172
XmiI GTMKAC 2 cut(s) 282, 306
XmnI GAANNNNTTC 1 cut(s) 1406
XspI CTAG 1 cut(s) 1110
ZraI GACGTC 1 cut(s) 790
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.