MD13G1093000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016320
Physical Location & Seq
Reverse (-)
6559081 .. 6559623
543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1093000.v1.1.491

Sequence Viewer

Length: 543 bp
ATGTCCTCCTTGCAGCCTCAGCAGCAGCAGCAACAGCCTTTTGGCCCTGTTCTTGTTTACCCTAACGATGTCAATGGACAGCCTTCATCTTCCCACCATTCAAATGGATCATTCGGGACTGTTTTCATCGTGCTGGCCGTTATAATCGTCATCTCCGCCATCGCTTGCTTTCTCGGAAGGCTCTGCAATCGCCGCCACAACAGCTCAAAACCCCATAAGCAACCGCAACATAACTTTCGTCCCAAGAAAGAAGCTGGCCCTGCAGGTGGCGGTGGTGGAGACCATCGCGTGGAGTTTGGGAGAGCTCCCAGCTTTCGAGCAGGCCCGAAAGTAGCAGACGTCGAATTTGGATTCGATAAGAAAAACCCGAAAGGGAACAGCAGCAGCAGAGGAGAGGGCAGAGGATACATGAATCATCACCCCAACAAACCACACCATAACGGAAATGGTGAAAAGAATCGTCACATGAAAGGTGAAATGAAGCAGCATCCTGGCGGCGAGCATCATATTGGAATGGGAGATCCGAGCCAGTGGATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

19.69

Weight (kDa)

10.06

Isoelectric Point (pI)

56.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g34300
malus_domestica MD13G1093000.v1.1 MD16G1094000.v1.1
prunus_persica Prupe.1G252500_v2.0.a1
pyrus_communis pycom13g08100 pycom16g08000
rosa_chinensis RchiOBHm_Chr4g0443731
rosa_laevigata RLG00000005895
rosa_multiflora Rmu_sc0001426.1_g000003
rosa_roxburghii Rroxscaffold_5G00384300
rosa_samantha Rh4AG407000 Rh4BG417900 Rh4CG432100 Rh4DG413200
rosa_wichuraiana Rw4G034950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
AarI CACCTGC 1 cut(s) 254
AatII GACGTC 1 cut(s) 342
Acc36I ACCTGC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 289
AccII CGCG 1 cut(s) 288
AciI CCGC 5 cut(s) 156, 193, 224, 270, 495
AclWI GGATC 2 cut(s) 115, 515
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 339
AfiI CCNNNNNNNGG 2 cut(s) 266, 289
AgsI TTSAA 1 cut(s) 102
AjnI CCWGG 1 cut(s) 490
AluBI AGCT 4 cut(s) 204, 254, 305, 312
AluI AGCT 4 cut(s) 204, 254, 305, 312
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 1 cut(s) 273
AlwI GGATC 2 cut(s) 115, 515
AoxI GGCC 4 cut(s) 43, 135, 256, 322
ApeKI GCWGC 7 cut(s) 13, 22, 25, 28, 381, 384, 484
ApoI RAATTY 1 cut(s) 344
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspS9I GGNCC 3 cut(s) 44, 257, 323
AsuHPI GGTGA 3 cut(s) 410, 461, 485
BanII GRGCYC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 307
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 7 cut(s) 25, 34, 37, 40, 393, 396, 496
BccI CCATC 2 cut(s) 167, 291
BceAI ACGGC 1 cut(s) 122
BciT130I CCWGG 1 cut(s) 492
BciVI GTATCC 1 cut(s) 398
BcoDI GTCTC 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 261
BfuAI ACCTGC 1 cut(s) 254
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 9 cut(s) 14, 23, 26, 29, 193, 382, 385, 485, 496
BlsI GCNGC 9 cut(s) 15, 24, 27, 30, 194, 383, 386, 486, 497
Bme1390I CCNGG 1 cut(s) 492
BmgT120I GGNCC 3 cut(s) 44, 257, 323
BmrFI CCNGG 1 cut(s) 492
BmsI GCATC 2 cut(s) 496, 511
Bpu10I CCTNAGC 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 273
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 289
Bse1I ACTGG 1 cut(s) 529
BseBI CCWGG 1 cut(s) 492
BseGI GGATG 2 cut(s) 487, 540
BseLI CCNNNNNNNGG 2 cut(s) 266, 289
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 529
BseRI GAGGAG 1 cut(s) 405
BseXI GCAGC 7 cut(s) 25, 34, 37, 40, 393, 396, 496
BseYI CCCAGC 1 cut(s) 308
Bsh1236I CGCG 1 cut(s) 288
BshFI GGCC 4 cut(s) 45, 137, 258, 324
BsiHKAI GWGCWC 1 cut(s) 307
BslFI GGGAC 2 cut(s) 130, 225
BslI CCNNNNNNNGG 2 cut(s) 266, 289
BsmAI GTCTC 1 cut(s) 273
BsmFI GGGAC 2 cut(s) 130, 225
BsnI GGCC 4 cut(s) 45, 137, 258, 324
Bso31I GGTCTC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 2 cut(s) 107, 520
BspACI CCGC 5 cut(s) 156, 193, 224, 270, 495
BspANI GGCC 4 cut(s) 45, 137, 258, 324
BspCNI CTCAG 1 cut(s) 31
BspFNI CGCG 1 cut(s) 288
BspMAI CTGCAG 1 cut(s) 265
BspMI ACCTGC 1 cut(s) 254
BspPI GGATC 2 cut(s) 115, 515
BspTNI GGTCTC 1 cut(s) 273
BsrI ACTGG 1 cut(s) 529
BssMI GATC 2 cut(s) 107, 520
BssNI GRCGYC 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 1 cut(s) 121
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 5 cut(s) 135, 166, 256, 322, 500
BstDEI CTNAG 1 cut(s) 18
BstF5I GGATG 2 cut(s) 487, 540
BstFNI CGCG 1 cut(s) 288
BstKTI GATC 2 cut(s) 110, 523
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 2 cut(s) 107, 520
BstMWI GCNNNNNNNGC 7 cut(s) 19, 22, 28, 34, 192, 201, 260
BstNI CCWGG 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 490
BstSFI CTRYAG 1 cut(s) 261
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 7 cut(s) 25, 34, 37, 40, 393, 396, 496
BstX2I RGATCY 1 cut(s) 520
BstXI CCANNNNNNTGG 1 cut(s) 104
BstYI RGATCY 1 cut(s) 520
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 4 cut(s) 45, 137, 258, 324
BtgZI GCGATG 2 cut(s) 145, 269
BtsCI GGATG 2 cut(s) 487, 540
BtsIMutI CAGTG 1 cut(s) 536
BveI ACCTGC 1 cut(s) 254
Cac8I GCNNGC 5 cut(s) 135, 166, 256, 322, 500
Cfr13I GGNCC 3 cut(s) 44, 257, 323
CviAII CATG 2 cut(s) 409, 466
DdeI CTNAG 1 cut(s) 18
DpnI GATC 2 cut(s) 109, 522
DpnII GATC 2 cut(s) 107, 520
EaeI YGGCCR 1 cut(s) 135
EciI GGCGGA 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 307
Eco31I GGTCTC 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 307
FaeI CATG 2 cut(s) 412, 469
FaiI YATR 7 cut(s) 143, 216, 231, 410, 438, 467, 507
FaqI GGGAC 2 cut(s) 130, 225
FatI CATG 2 cut(s) 408, 465
Fnu4HI GCNGC 9 cut(s) 14, 23, 26, 29, 193, 382, 385, 485, 496
FokI GGATG 1 cut(s) 474
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 9 cut(s) 14, 23, 26, 29, 193, 382, 385, 485, 496
GluI GCNGC 9 cut(s) 14, 23, 26, 29, 193, 382, 385, 485, 496
GsaI CCCAGC 1 cut(s) 312
HaeIII GGCC 4 cut(s) 45, 137, 258, 324
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 2 cut(s) 412, 469
HinfI GANTC 3 cut(s) 351, 412, 457
HphI GGTGA 3 cut(s) 410, 461, 485
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 2 cut(s) 176, 525
Hpy188III TCNNGA 1 cut(s) 115
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 2 cut(s) 93, 171
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 3 cut(s) 13, 186, 263
HpyF10VI GCNNNNNNNGC 7 cut(s) 19, 22, 28, 34, 192, 201, 260
HpyF3I CTNAG 1 cut(s) 18
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 2 cut(s) 412, 469
Kzo9I GATC 2 cut(s) 107, 520
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 9 cut(s) 60, 119, 240, 249, 273, 306, 322, 477, 504
Lsp1109I GCAGC 7 cut(s) 25, 34, 37, 40, 393, 396, 496
LweI GCATC 2 cut(s) 496, 511
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 461
MalI GATC 2 cut(s) 109, 522
MboI GATC 2 cut(s) 107, 520
MboII GAAGA 1 cut(s) 81
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 1 cut(s) 344
MnlI CCTC 5 cut(s) 16, 27, 383, 388, 395
MslI CAYNNNNRTG 1 cut(s) 102
MspR9I CCNGG 1 cut(s) 492
MvaI CCWGG 1 cut(s) 492
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 7 cut(s) 19, 22, 28, 34, 192, 201, 260
NdeII GATC 2 cut(s) 107, 520
NlaIII CATG 2 cut(s) 412, 469
NmuCI GTSAC 1 cut(s) 461
PaqCI CACCTGC 1 cut(s) 254
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 3 cut(s) 351, 412, 457
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 9 cut(s) 15, 24, 27, 30, 194, 383, 386, 486, 497
PsiI TTATAA 1 cut(s) 143
Psp124BI GAGCTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 490
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 1 cut(s) 490
PspPI GGNCC 3 cut(s) 44, 257, 323
PstI CTGCAG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 520
RseI CAYNNNNRTG 1 cut(s) 102
SacI GAGCTC 1 cut(s) 307
SatI GCNGC 9 cut(s) 14, 23, 26, 29, 193, 382, 385, 485, 496
Sau3AI GATC 2 cut(s) 107, 520
Sau96I GGNCC 3 cut(s) 44, 257, 323
SbfI CCTGCAGG 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 492
SdaI CCTGCAGG 1 cut(s) 265
SduI GDGCHC 1 cut(s) 307
SetI ASST 7 cut(s) 206, 256, 268, 307, 314, 342, 475
SfaNI GCATC 2 cut(s) 496, 511
SfcI CTRYAG 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 102
Sse8387I CCTGCAGG 1 cut(s) 265
Sse9I AATT 1 cut(s) 344
SsiI CCGC 5 cut(s) 156, 193, 224, 270, 495
SstI GAGCTC 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 490
TaaI ACNGT 1 cut(s) 121
TaiI ACGT 1 cut(s) 342
TaqI TCGA 3 cut(s) 316, 342, 354
TasI AATT 1 cut(s) 344
TauI GCSGC 2 cut(s) 195, 498
TfiI GAWTC 3 cut(s) 351, 412, 457
TscAI CASTG 1 cut(s) 536
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 7 cut(s) 13, 22, 25, 28, 381, 384, 484
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 5 cut(s) 75, 115, 425, 482, 494
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 1 cut(s) 536
Van91I CCANNNNNTGG 1 cut(s) 289
XapI RAATTY 1 cut(s) 344
XcmI CCANNNNNNNNNTGG 2 cut(s) 101, 443
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.