MD13G1107700.v1.1

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010085
Physical Location & Seq
Reverse (-)
7714049 .. 7716242
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1107700.v1.1.491

Sequence Viewer

Length: 684 bp
ATGACTATGCTTCTTGCTTTGAGAGTTGCAATTAGCCCGAGAGCTTTTGCAAGTCGGAATTCGGTGAAGAAATTGAGAAGGGATGGACAGGCACGAAAGAGGGTAGCGGATAAGAGCATTGCTTCTGAAGATGATAGTGTTAAAAATGACAAGAAAGTGGAATCTTCTGATAATTTAGCTGCAGAGGAATTCCTTGTATTTCCTTCTAGAGGTGCTGTGCTTCGGGCTTGCACTGTTACATCCGGGTTGATAGCGGCTTTGGGTATAATTATTCGACAGGCATCTCATGTTGCATCAGTTGAAGGATTGCCAGTCCTTGACTGCTCTTTGGAATTGATTTCGGGACTGGTCATTCTGATATCACTTAGCCGATATCTATTATTGAAGACATGGCCGGATTTCGCAGAGTCCAGCAAGGAACTTCTTTTTCGGGGTGCCTTGCTACCACTTTTTGGATCCAATTGGAGGAGCGCCTTGATAGTCGCCATCATTTTTGGTGTTCTACACCTGGGCAGTGGCCGAAAGTATTCCTTCGCTGTCTGGGCAACTTTTGTGGGTCTTGTGTATGGTTATGCAACCATCGTCTCCTCTAGCCTTATCGTGCCAATGGCATCTCATGCAGTCAACAATTTTGTTGGAGGGATGTTATGGCGTTACCGATCAGATTCATCACGGAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

228

Amino Acids

24.72

Weight (kDa)

10.0

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 140 - 212 3.2e-14 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 434
AccB7I CCANNNNNTGG 1 cut(s) 452
AciI CCGC 2 cut(s) 107, 254
AclWI GGATC 2 cut(s) 450, 463
AcoI YGGCCR 2 cut(s) 392, 517
AcsI RAATTY 2 cut(s) 58, 188
AcuI CTGAAG 1 cut(s) 147
AfiI CCNNNNNNNGG 3 cut(s) 209, 452, 465
AgsI TTSAA 2 cut(s) 302, 385
AjnI CCWGG 1 cut(s) 507
AluBI AGCT 2 cut(s) 44, 179
AluI AGCT 2 cut(s) 44, 179
Alw26I GTCTC 1 cut(s) 589
AlwI GGATC 2 cut(s) 450, 463
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 2 cut(s) 392, 517
ApeKI GCWGC 1 cut(s) 179
ApoI RAATTY 2 cut(s) 58, 188
Asp700I GAANNNNTTC 1 cut(s) 526
AspLEI GCGC 1 cut(s) 473
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 37
BamHI GGATCC 1 cut(s) 455
BanI GGYRCC 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 392
BbvI GCAGC 1 cut(s) 166
BccI CCATC 3 cut(s) 77, 494, 587
BciT130I CCWGG 1 cut(s) 509
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 589
BfaI CTAG 2 cut(s) 207, 591
BfmI CTRYAG 1 cut(s) 180
BfoI RGCGCY 1 cut(s) 474
BisI GCNGC 2 cut(s) 180, 255
BlsI GCNGC 2 cut(s) 181, 256
Bme1390I CCNGG 2 cut(s) 244, 509
BmeT110I CYCGRG 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 436, 457
BmrFI CCNGG 2 cut(s) 244, 509
BmsI GCATC 3 cut(s) 290, 302, 620
BpiI GAAGAC 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 508
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 452, 465
Bse1I ACTGG 2 cut(s) 311, 351
Bse3DI GCAATG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 3 cut(s) 88, 239, 648
BseLI CCNNNNNNNGG 3 cut(s) 209, 452, 465
BseMI GCAATG 1 cut(s) 117
BseNI ACTGG 2 cut(s) 311, 351
BseRI GAGGAG 2 cut(s) 481, 577
BseXI GCAGC 1 cut(s) 166
BshFI GGCC 2 cut(s) 394, 519
BshNI GGYRCC 1 cut(s) 434
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 2 cut(s) 243, 395
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 3 cut(s) 209, 452, 465
BsmAI GTCTC 1 cut(s) 589
BsmBI CGTCTC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 2 cut(s) 394, 519
BsoBI CYCGRG 1 cut(s) 37
Bsp143I GATC 2 cut(s) 455, 659
BspACI CCGC 2 cut(s) 107, 254
BspANI GGCC 2 cut(s) 394, 519
BspLI GGNNCC 2 cut(s) 436, 457
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 2 cut(s) 450, 463
BspT107I GGYRCC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 117
BsrI ACTGG 2 cut(s) 311, 351
BssECI CCNNGG 1 cut(s) 508
BssMI GATC 2 cut(s) 455, 659
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 1 cut(s) 235
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 1 cut(s) 365
BstENI CCTNNNNNAGG 1 cut(s) 207
BstF5I GGATG 3 cut(s) 88, 239, 648
BstH2I RGCGCY 1 cut(s) 474
BstHHI GCGC 1 cut(s) 473
BstKTI GATC 2 cut(s) 458, 662
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 2 cut(s) 455, 659
BstMWI GCNNNNNNNGC 2 cut(s) 542, 617
BstNI CCWGG 1 cut(s) 509
BstSCI CCNGG 2 cut(s) 242, 507
BstSFI CTRYAG 1 cut(s) 180
BstV1I GCAGC 1 cut(s) 166
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 1 cut(s) 455
BstYI RGATCY 1 cut(s) 455
BsuRI GGCC 2 cut(s) 394, 519
BtsCI GGATG 3 cut(s) 88, 239, 648
BtsI GCAGTG 1 cut(s) 520
BtsIMutI CAGTG 2 cut(s) 231, 520
Cac8I GCNNGC 1 cut(s) 229
CfoI GCGC 1 cut(s) 473
CspCI CAANNNNNGTGG 2 cut(s) 534, 569
CviAII CATG 3 cut(s) 287, 390, 617
CviJI RGCY 9 cut(s) 36, 44, 179, 227, 257, 369, 394, 519, 594
CviKI_1 RGCY 9 cut(s) 36, 44, 179, 227, 257, 369, 394, 519, 594
DdeI CTNAG 1 cut(s) 365
DpnI GATC 2 cut(s) 457, 661
DpnII GATC 2 cut(s) 455, 659
EaeI YGGCCR 2 cut(s) 392, 517
Eco32I GATATC 2 cut(s) 360, 374
Eco57I CTGAAG 1 cut(s) 147
Eco88I CYCGRG 1 cut(s) 37
EcoNI CCTNNNNNAGG 1 cut(s) 207
EcoRI GAATTC 2 cut(s) 58, 188
EcoRII CCWGG 1 cut(s) 507
EcoRV GATATC 2 cut(s) 360, 374
Esp3I CGTCTC 1 cut(s) 589
FaeI CATG 3 cut(s) 290, 393, 620
FaiI YATR 9 cut(s) 8, 266, 288, 391, 567, 573, 618, 649, 682
FalI AAGNNNNNCTT 2 cut(s) 515, 547
FaqI GGGAC 1 cut(s) 357
FatI CATG 3 cut(s) 286, 389, 616
Fnu4HI GCNGC 2 cut(s) 180, 255
FokI GGATG 3 cut(s) 95, 226, 655
Fsp4HI GCNGC 2 cut(s) 180, 255
FspBI CTAG 2 cut(s) 207, 591
GlaI GCGC 1 cut(s) 472
GluI GCNGC 2 cut(s) 180, 255
HaeII RGCGCY 1 cut(s) 474
HaeIII GGCC 2 cut(s) 394, 519
HapII CCGG 2 cut(s) 243, 395
HhaI GCGC 1 cut(s) 473
Hin1II CATG 3 cut(s) 290, 393, 620
Hin6I GCGC 1 cut(s) 471
HinP1I GCGC 1 cut(s) 471
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 3 cut(s) 161, 407, 665
HpaII CCGG 2 cut(s) 243, 395
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 5 cut(s) 57, 127, 169, 357, 664
Hpy188III TCNNGA 2 cut(s) 207, 342
Hpy8I GTNNAC 1 cut(s) 625
HpyAV CCTTC 4 cut(s) 72, 213, 296, 541
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4V TGCA 7 cut(s) 29, 50, 182, 231, 293, 575, 620
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 617
HpyF3I CTNAG 1 cut(s) 365
Hsp92II CATG 3 cut(s) 290, 393, 620
HspAI GCGC 1 cut(s) 471
Kzo9I GATC 2 cut(s) 455, 659
LmnI GCTCC 1 cut(s) 468
Lsp1109I GCAGC 1 cut(s) 166
LweI GCATC 3 cut(s) 290, 302, 620
MaeI CTAG 2 cut(s) 207, 591
MaeIII GTNAC 2 cut(s) 235, 653
MalI GATC 2 cut(s) 457, 661
MboI GATC 2 cut(s) 455, 659
MboII GAAGA 4 cut(s) 79, 140, 156, 397
MfeI CAATTG 1 cut(s) 460
MflI RGATCY 1 cut(s) 455
MluCI AATT 9 cut(s) 30, 58, 71, 172, 188, 267, 332, 460, 628
MlyI GAGTC 1 cut(s) 416
MmeI TCCRAC 2 cut(s) 35, 616
MnlI CCTC 6 cut(s) 93, 178, 203, 459, 598, 632
MroXI GAANNNNTTC 1 cut(s) 526
MseI TTAA 1 cut(s) 141
MspI CCGG 2 cut(s) 243, 395
MspR9I CCNGG 2 cut(s) 244, 509
MunI CAATTG 1 cut(s) 460
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 2 cut(s) 542, 617
NciI CCSGG 1 cut(s) 244
NdeII GATC 2 cut(s) 455, 659
NlaIII CATG 3 cut(s) 290, 393, 620
NlaIV GGNNCC 2 cut(s) 436, 457
PdmI GAANNNNTTC 1 cut(s) 526
PfeI GAWTC 2 cut(s) 161, 665
PflMI CCANNNNNTGG 1 cut(s) 452
PkrI GCNGC 2 cut(s) 181, 256
PleI GAGTC 1 cut(s) 415
PpsI GAGTC 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 507
PspGI CCWGG 1 cut(s) 507
PspN4I GGNNCC 2 cut(s) 436, 457
PstI CTGCAG 1 cut(s) 184
PsuI RGATCY 1 cut(s) 455
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 2 cut(s) 180, 255
Sau3AI GATC 2 cut(s) 455, 659
SchI GAGTC 1 cut(s) 416
ScrFI CCNGG 2 cut(s) 244, 509
SetI ASST 4 cut(s) 46, 181, 214, 510
SfaNI GCATC 3 cut(s) 290, 302, 620
SfcI CTRYAG 1 cut(s) 180
Sse9I AATT 9 cut(s) 30, 58, 71, 172, 188, 267, 332, 460, 628
SsiI CCGC 2 cut(s) 107, 254
SspMI CTAG 2 cut(s) 207, 591
StyD4I CCNGG 2 cut(s) 242, 507
TaaI ACNGT 1 cut(s) 235
TaqI TCGA 1 cut(s) 274
TasI AATT 9 cut(s) 30, 58, 71, 172, 188, 267, 332, 460, 628
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 2 cut(s) 161, 665
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 2 cut(s) 238, 520
TseI GCWGC 1 cut(s) 179
TspDTI ATGAA 1 cut(s) 657
TspRI CASTG 2 cut(s) 238, 520
Van91I CCANNNNNTGG 1 cut(s) 452
XagI CCTNNNNNAGG 1 cut(s) 207
XapI RAATTY 2 cut(s) 58, 188
XbaI TCTAGA 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 526
XspI CTAG 2 cut(s) 207, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.