Rroxscaffold_5G00373550

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010085
Physical Location & Seq
Forward (+)
54745390 .. 54749043
3654 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00373550.1

Sequence Viewer

Length: 903 bp
ATGGGTTGGCTTACAGTAAACAATGCATCTACAACCTCATTGACTTGGGGGAACTCTATCTTCACTTGTGGGAAGTGTATTGGCCTCTATGATGTGCAAAAGTGTTTGCCAGCCAAATCATGCAGTGGAGTTGCATTTAGCCCAAGAGCTTTTGCAAGCCGGAATTCGCTGAAGAAGTTGAGAAGGGAGAGACTAGCAAGCGAAAGGGTAGCAGATAAAACTACTGCTAGTTCTGAAAATGTTCAAAATGACAACAAGGTGGAGCTTTCTGATGATTCAGCTGCAGAGAAAGGGGTTAATATCCCTTCCAGAGGTGCTGTGCTTCAGGCTTGCACTGTTACTTCCGGGTTGATAGCTGCTTTGGGTATACTAATTCGACAGGCATCTCATGTTGCATCGGTTGAAGGATTACCAGTCCTTGACTGCTCATTGGAAGTATCATTTGATTTTGAGATGTGGCATCTTCAGTTGATTGCCGGATTGGTTATTTTGATATCATCATCCCGATATATATTACTGAAGACATGGCCAGAATTTGCAGAGTCTAGCAAAGCAGCCAATCAACAGGTCCTTACTTCACTTCAACCTTTGGATTTCATAATCGTTGCATTTTTACCAGGGGTCAGTGAGGAACTTCTTTTCCGGGGTGCGCTGCAGCCTCTTTTTGGATCCAATTTGAGTAGTGCATTGGTGGTTGCCCTCGTCTTTGGTGTTCTACACCTGGGCAGTGGCCGAAAGTATTCCTTCGCAGTCTGGGCAAGTTTGGTTGGGTTTGTGTATGGTTACGCAGCCATTGCATCTTCTAGCCTTGTTGTGCCAATGGTTTCTCATGCAGTGAACAATTTGTTGGGAGGGATTTTATGGCGATACAGTTCAGATTCATCAGGACAGATATCAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

300

Amino Acids

32.05

Weight (kDa)

7.61

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 195 - 283 1.4e-17 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 367
AclWI GGATC 2 cut(s) 663, 676
AcoI YGGCCR 2 cut(s) 527, 730
AcsI RAATTY 2 cut(s) 163, 533
AcuI CTGAAG 4 cut(s) 191, 308, 449, 539
AfiI CCNNNNNNNGG 2 cut(s) 311, 665
AgsI TTSAA 3 cut(s) 245, 404, 584
AjnI CCWGG 2 cut(s) 616, 720
AjuI GAANNNNNNNTTGG 2 cut(s) 830, 862
AloI GAACNNNNNNTCC 2 cut(s) 624, 656
AluBI AGCT 4 cut(s) 149, 265, 281, 356
AluI AGCT 4 cut(s) 149, 265, 281, 356
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 2 cut(s) 663, 676
AoxI GGCC 3 cut(s) 82, 527, 730
ApeKI GCWGC 6 cut(s) 281, 356, 554, 652, 655, 788
ApoI RAATTY 2 cut(s) 163, 533
Asp700I GAANNNNTTC 2 cut(s) 240, 739
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 1 cut(s) 568
AsuC2I CCSGG 2 cut(s) 346, 644
AvaII GGWCC 1 cut(s) 568
BalI TGGCCA 1 cut(s) 529
BamHI GGATCC 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 527
BbvI GCAGC 6 cut(s) 268, 343, 566, 639, 667, 800
BciT130I CCWGG 2 cut(s) 618, 722
BcnI CCSGG 2 cut(s) 346, 644
BcoDI GTCTC 1 cut(s) 184
BfaI CTAG 4 cut(s) 194, 228, 546, 804
BfmI CTRYAG 2 cut(s) 282, 653
BisI GCNGC 6 cut(s) 282, 357, 555, 653, 656, 789
BlsI GCNGC 6 cut(s) 283, 358, 556, 654, 657, 790
Bme1390I CCNGG 4 cut(s) 346, 618, 644, 722
Bme18I GGWCC 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 568
BmiI GGNNCC 1 cut(s) 670
BmrFI CCNGG 4 cut(s) 346, 618, 644, 722
BmsI GCATC 5 cut(s) 35, 392, 404, 469, 806
BpiI GAAGAC 1 cut(s) 527
BpuMI CCSGG 2 cut(s) 346, 644
BsaJI CCNNGG 3 cut(s) 617, 643, 721
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 665
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 2 cut(s) 618, 722
BseDI CCNNGG 3 cut(s) 617, 643, 721
BseGI GGATG 1 cut(s) 500
BseLI CCNNNNNNNGG 2 cut(s) 311, 665
BseMI GCAATG 1 cut(s) 792
BseNI ACTGG 1 cut(s) 413
BseXI GCAGC 6 cut(s) 268, 343, 566, 639, 667, 800
BshFI GGCC 3 cut(s) 84, 529, 732
BsiSI CCGG 4 cut(s) 160, 345, 477, 643
BslI CCNNNNNNNGG 2 cut(s) 311, 665
BsmAI GTCTC 1 cut(s) 184
BsnI GGCC 3 cut(s) 84, 529, 732
Bsp143I GATC 1 cut(s) 668
BspANI GGCC 3 cut(s) 84, 529, 732
BspLI GGNNCC 1 cut(s) 670
BspMAI CTGCAG 2 cut(s) 286, 657
BspPI GGATC 2 cut(s) 663, 676
BsrDI GCAATG 1 cut(s) 792
BsrI ACTGG 1 cut(s) 413
BssECI CCNNGG 3 cut(s) 617, 643, 721
BssMI GATC 1 cut(s) 668
BssNAI GTATAC 1 cut(s) 368
Bst1107I GTATAC 1 cut(s) 368
Bst2UI CCWGG 2 cut(s) 618, 722
Bst4CI ACNGT 3 cut(s) 16, 337, 872
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 4 cut(s) 111, 157, 199, 331
BstENI CCTNNNNNAGG 1 cut(s) 309
BstF5I GGATG 1 cut(s) 500
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 1 cut(s) 671
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 1 cut(s) 668
BstMWI GCNNNNNNNGC 2 cut(s) 755, 794
BstNI CCWGG 2 cut(s) 618, 722
BstSCI CCNGG 4 cut(s) 344, 616, 642, 720
BstSFI CTRYAG 2 cut(s) 282, 653
BstV1I GCAGC 6 cut(s) 268, 343, 566, 639, 667, 800
BstV2I GAAGAC 1 cut(s) 527
BstX2I RGATCY 1 cut(s) 668
BstYI RGATCY 1 cut(s) 668
BstZ17I GTATAC 1 cut(s) 368
BsuRI GGCC 3 cut(s) 84, 529, 732
BtsCI GGATG 1 cut(s) 500
BtsI GCAGTG 3 cut(s) 130, 733, 840
BtsIMutI CAGTG 5 cut(s) 130, 333, 631, 733, 840
Cac8I GCNNGC 4 cut(s) 111, 157, 199, 331
CfoI GCGC 1 cut(s) 652
Cfr13I GGNCC 1 cut(s) 568
CviAII CATG 4 cut(s) 120, 389, 525, 830
DpnI GATC 1 cut(s) 670
DpnII GATC 1 cut(s) 668
EaeI YGGCCR 2 cut(s) 527, 730
Eco32I GATATC 2 cut(s) 495, 894
Eco47I GGWCC 1 cut(s) 568
Eco57I CTGAAG 4 cut(s) 191, 308, 449, 539
EcoNI CCTNNNNNAGG 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 568
EcoRI GAATTC 1 cut(s) 163
EcoRII CCWGG 2 cut(s) 616, 720
EcoRV GATATC 2 cut(s) 495, 894
EcoT22I ATGCAT 1 cut(s) 28
FaeI CATG 4 cut(s) 123, 392, 528, 833
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FatI CATG 4 cut(s) 119, 388, 524, 829
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 6 cut(s) 282, 357, 555, 653, 656, 789
FokI GGATG 1 cut(s) 487
Fsp4HI GCNGC 6 cut(s) 282, 357, 555, 653, 656, 789
FspBI CTAG 4 cut(s) 194, 228, 546, 804
GlaI GCGC 1 cut(s) 651
GluI GCNGC 6 cut(s) 282, 357, 555, 653, 656, 789
HaeIII GGCC 3 cut(s) 84, 529, 732
HapII CCGG 4 cut(s) 160, 345, 477, 643
HhaI GCGC 1 cut(s) 652
Hin1II CATG 4 cut(s) 123, 392, 528, 833
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HinfI GANTC 3 cut(s) 275, 542, 878
HpaII CCGG 4 cut(s) 160, 345, 477, 643
Hpy166II GTNNAC 3 cut(s) 19, 368, 838
Hpy188I TCNGA 4 cut(s) 235, 271, 877, 898
Hpy188III TCNNGA 3 cut(s) 309, 504, 885
Hpy8I GTNNAC 3 cut(s) 19, 368, 838
HpyAV CCTTC 4 cut(s) 177, 315, 398, 754
HpyCH4III ACNGT 3 cut(s) 16, 337, 872
HpyF10VI GCNNNNNNNGC 2 cut(s) 755, 794
Hsp92II CATG 4 cut(s) 123, 392, 528, 833
HspAI GCGC 1 cut(s) 650
Kzo9I GATC 1 cut(s) 668
LmnI GCTCC 1 cut(s) 262
Lsp1109I GCAGC 6 cut(s) 268, 343, 566, 639, 667, 800
LweI GCATC 5 cut(s) 35, 392, 404, 469, 806
MaeI CTAG 4 cut(s) 194, 228, 546, 804
MaeIII GTNAC 2 cut(s) 337, 782
MalI GATC 1 cut(s) 670
MboI GATC 1 cut(s) 668
MboII GAAGA 5 cut(s) 52, 184, 455, 532, 792
MflI RGATCY 1 cut(s) 668
MlsI TGGCCA 1 cut(s) 529
MluCI AATT 5 cut(s) 163, 372, 533, 673, 841
MluNI TGGCCA 1 cut(s) 529
MlyI GAGTC 1 cut(s) 551
MnlI CCTC 7 cut(s) 46, 95, 305, 622, 669, 710, 845
Mox20I TGGCCA 1 cut(s) 529
Mph1103I ATGCAT 1 cut(s) 28
MroXI GAANNNNTTC 2 cut(s) 240, 739
MscI TGGCCA 1 cut(s) 529
MseI TTAA 2 cut(s) 297, 901
Msp20I TGGCCA 1 cut(s) 529
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 4 cut(s) 160, 345, 477, 643
MspR9I CCNGG 4 cut(s) 346, 618, 644, 722
MvaI CCWGG 2 cut(s) 618, 722
MwoI GCNNNNNNNGC 2 cut(s) 755, 794
NciI CCSGG 2 cut(s) 346, 644
NdeII GATC 1 cut(s) 668
NlaIII CATG 4 cut(s) 123, 392, 528, 833
NlaIV GGNNCC 1 cut(s) 670
NsiI ATGCAT 1 cut(s) 28
PdmI GAANNNNTTC 2 cut(s) 240, 739
PfeI GAWTC 2 cut(s) 275, 878
PkrI GCNGC 6 cut(s) 283, 358, 556, 654, 657, 790
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
PpuMI RGGWCCY 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 2 cut(s) 616, 720
PspGI CCWGG 2 cut(s) 616, 720
PspN4I GGNNCC 1 cut(s) 670
PspPI GGNCC 1 cut(s) 568
PspPPI RGGWCCY 1 cut(s) 568
PstI CTGCAG 2 cut(s) 286, 657
PsuI RGATCY 1 cut(s) 668
PvuII CAGCTG 1 cut(s) 281
SaqAI TTAA 2 cut(s) 297, 901
SatI GCNGC 6 cut(s) 282, 357, 555, 653, 656, 789
Sau3AI GATC 1 cut(s) 668
Sau96I GGNCC 1 cut(s) 568
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 4 cut(s) 346, 618, 644, 722
SfaNI GCATC 5 cut(s) 35, 392, 404, 469, 806
SfcI CTRYAG 2 cut(s) 282, 653
SinI GGWCC 1 cut(s) 568
Sse9I AATT 5 cut(s) 163, 372, 533, 673, 841
SspMI CTAG 4 cut(s) 194, 228, 546, 804
StyD4I CCNGG 4 cut(s) 344, 616, 642, 720
TaaI ACNGT 3 cut(s) 16, 337, 872
TaqI TCGA 1 cut(s) 376
TasI AATT 5 cut(s) 163, 372, 533, 673, 841
TfiI GAWTC 2 cut(s) 275, 878
Tru1I TTAA 2 cut(s) 297, 901
Tru9I TTAA 2 cut(s) 297, 901
TscAI CASTG 5 cut(s) 130, 340, 631, 733, 840
TseI GCWGC 6 cut(s) 281, 356, 554, 652, 655, 788
TspDTI ATGAA 2 cut(s) 586, 870
TspRI CASTG 5 cut(s) 130, 340, 631, 733, 840
VpaK11BI GGWCC 1 cut(s) 568
XagI CCTNNNNNAGG 1 cut(s) 309
XapI RAATTY 2 cut(s) 163, 533
XmiI GTMKAC 1 cut(s) 367
XmnI GAANNNNTTC 2 cut(s) 240, 739
XspI CTAG 4 cut(s) 194, 228, 546, 804
Zsp2I ATGCAT 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.