MD16G1107600.v1.1

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010085
Physical Location & Seq
Reverse (-)
7535256 .. 7538072
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1107600.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGGTTCCCTTTCTGTGATCTCATTGAGTTGGGGAAGCTCTATCTTCATTGGTGGGAAGTTTGATGTGCACAAAGCTTTGCCTCCCAAATTATGTAGTGGAGTTGCAATTAGCCCGAGAGCTTTTGCAAGTCAGAATTCGGTGAAGAAATTGAGAAGGGACGGGCAGGCACGAAAAAAGGTAGCAGATAAGAGCATTGCTTCCGAAGATGATTGTGTTAAAAATGACGAGAATGTGGAATCTTCTGTTAATTTAGCTGCAGAGGAACTGGTTGCATTCCCTTCCAGAGGTGCTGTGCTTCAGGCTTGCACTGTTACATCCGGGTTGATAGCGGCTTTGGGTATAATTATTCGACAGGCATCTCATGTTGCAAAGATTGAAGGATTGCCAGTCTTTGACTGCTCATTGGAAGTACCATTTGATTTTGAGGTGTGGCATCTTCAGTTGATTTCTGGATTGGTTATTCTGATATCACTTAGCCGATATCTATTATTGAAGACATGGCCGGATTTCGCGGAGTCCAGCGAAGCAGCCAATCAGCAGGTCCTTACATCACTTCAACCTTTGGACTACATAATAGTTGCATTTTTGCCTGGGGTCAGCGAGGAACTTCTTTTTCGGGGTGCCTTGCTACCACTTTTTGGATCTAATTGGAGGAGTGCCTTGGTAGTCGCCATCATTTTTGGTGTTCTACACCTGGGCAGTGGCCGAAAGTATTCCTTCGCTGTTTGGGCAACTTTTGTGGGTCTTGTGTATGGTTATGCAACCGTCGTCTCCTCTAGCCTTATCGTGCCAATGGCTTCTCATGCAGTCAACAATTTGGTTGGAGGGATGTTATGGCGTTACCGATCAGATTCATCACGGAAGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

291

Amino Acids

31.31

Weight (kDa)

8.64

Isoelectric Point (pI)

42.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 188 - 275 3.4e-18 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 623
AccB7I CCANNNNNTGG 1 cut(s) 641
AccII CGCG 1 cut(s) 515
AciI CCGC 2 cut(s) 332, 515
AclWI GGATC 1 cut(s) 652
AcoI YGGCCR 2 cut(s) 503, 706
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 2 cut(s) 284, 425
AfaI GTAC 1 cut(s) 414
AfiI CCNNNNNNNGG 2 cut(s) 287, 641
AgsI TTSAA 3 cut(s) 380, 496, 560
AjnI CCWGG 2 cut(s) 592, 696
AluBI AGCT 4 cut(s) 39, 77, 122, 257
AluI AGCT 4 cut(s) 39, 77, 122, 257
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 778
Alw44I GTGCAC 1 cut(s) 68
AlwI GGATC 1 cut(s) 652
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 2 cut(s) 503, 706
ApaLI GTGCAC 1 cut(s) 68
ApeKI GCWGC 2 cut(s) 257, 530
ApoI RAATTY 1 cut(s) 136
Asp700I GAANNNNTTC 1 cut(s) 715
AspS9I GGNCC 1 cut(s) 544
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 623
BbsI GAAGAC 1 cut(s) 503
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 2 cut(s) 244, 542
BccI CCATC 1 cut(s) 683
BciT130I CCWGG 2 cut(s) 594, 698
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 778
BfaI CTAG 1 cut(s) 780
BfmI CTRYAG 1 cut(s) 258
BfuAI ACCTGC 1 cut(s) 532
BisI GCNGC 3 cut(s) 258, 333, 531
BlsI GCNGC 3 cut(s) 259, 334, 532
Bme1390I CCNGG 3 cut(s) 322, 594, 698
Bme18I GGWCC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 2 cut(s) 7, 625
BmrFI CCNGG 3 cut(s) 322, 594, 698
BmsI GCATC 2 cut(s) 368, 445
BpiI GAAGAC 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 322
BsaJI CCNNGG 3 cut(s) 593, 663, 697
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 641
Bse1I ACTGG 2 cut(s) 273, 389
Bse3DI GCAATG 1 cut(s) 195
BseBI CCWGG 2 cut(s) 594, 698
BseDI CCNNGG 3 cut(s) 593, 663, 697
BseGI GGATG 2 cut(s) 317, 837
BseLI CCNNNNNNNGG 2 cut(s) 287, 641
BseMI GCAATG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 273, 389
BseRI GAGGAG 2 cut(s) 670, 766
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 2 cut(s) 244, 542
Bsh1236I CGCG 1 cut(s) 515
BshFI GGCC 2 cut(s) 505, 708
BshNI GGYRCC 1 cut(s) 623
BsiHKAI GWGCWC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 2 cut(s) 321, 506
BslFI GGGAC 1 cut(s) 173
BslI CCNNNNNNNGG 2 cut(s) 287, 641
BsmAI GTCTC 1 cut(s) 778
BsmBI CGTCTC 1 cut(s) 778
BsmFI GGGAC 1 cut(s) 173
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 2 cut(s) 505, 708
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 18, 644, 848
BspACI CCGC 2 cut(s) 332, 515
BspANI GGCC 2 cut(s) 505, 708
BspFNI CGCG 1 cut(s) 515
BspLI GGNNCC 2 cut(s) 7, 625
BspMAI CTGCAG 1 cut(s) 262
BspMI ACCTGC 1 cut(s) 532
BspPI GGATC 1 cut(s) 652
BspT107I GGYRCC 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 2 cut(s) 273, 389
BssECI CCNNGG 3 cut(s) 593, 663, 697
BssMI GATC 3 cut(s) 18, 644, 848
BssT1I CCWWGG 1 cut(s) 663
Bst2UI CCWGG 2 cut(s) 594, 698
Bst4CI ACNGT 2 cut(s) 313, 769
BstC8I GCNNGC 2 cut(s) 168, 307
BstDEI CTNAG 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 317, 837
BstFNI CGCG 1 cut(s) 515
BstKTI GATC 3 cut(s) 21, 647, 851
BstMAI GTCTC 1 cut(s) 778
BstMBI GATC 3 cut(s) 18, 644, 848
BstMWI GCNNNNNNNGC 2 cut(s) 731, 806
BstNI CCWGG 2 cut(s) 594, 698
BstSCI CCNGG 3 cut(s) 320, 592, 696
BstSFI CTRYAG 1 cut(s) 258
BstSLI GKGCMC 1 cut(s) 72
BstUI CGCG 1 cut(s) 515
BstV1I GCAGC 2 cut(s) 244, 542
BstV2I GAAGAC 1 cut(s) 503
BstX2I RGATCY 1 cut(s) 644
BstYI RGATCY 1 cut(s) 644
BsuRI GGCC 2 cut(s) 505, 708
BtsCI GGATG 2 cut(s) 317, 837
BtsI GCAGTG 1 cut(s) 709
BtsIMutI CAGTG 2 cut(s) 309, 709
BveI ACCTGC 1 cut(s) 532
Cac8I GCNNGC 2 cut(s) 168, 307
Cfr13I GGNCC 1 cut(s) 544
Csp6I GTAC 1 cut(s) 413
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 3 cut(s) 365, 501, 806
CviQI GTAC 1 cut(s) 413
DdeI CTNAG 1 cut(s) 476
DpnI GATC 3 cut(s) 20, 646, 850
DpnII GATC 3 cut(s) 18, 644, 848
EaeI YGGCCR 2 cut(s) 503, 706
Eco130I CCWWGG 1 cut(s) 663
Eco32I GATATC 2 cut(s) 471, 485
Eco47I GGWCC 1 cut(s) 544
Eco57I CTGAAG 2 cut(s) 284, 425
Eco88I CYCGRG 1 cut(s) 115
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoO109I RGGNCCY 1 cut(s) 544
EcoRI GAATTC 1 cut(s) 136
EcoRII CCWGG 2 cut(s) 592, 696
EcoRV GATATC 2 cut(s) 471, 485
EcoT14I CCWWGG 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 663
Esp3I CGTCTC 1 cut(s) 778
FaeI CATG 3 cut(s) 368, 504, 809
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 1 cut(s) 173
FatI CATG 3 cut(s) 364, 500, 805
Fnu4HI GCNGC 3 cut(s) 258, 333, 531
FokI GGATG 2 cut(s) 304, 844
Fsp4HI GCNGC 3 cut(s) 258, 333, 531
FspBI CTAG 1 cut(s) 780
GluI GCNGC 3 cut(s) 258, 333, 531
HaeIII GGCC 2 cut(s) 505, 708
HapII CCGG 2 cut(s) 321, 506
Hin1II CATG 3 cut(s) 368, 504, 809
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 3 cut(s) 239, 518, 854
HpaII CCGG 2 cut(s) 321, 506
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 70, 814
Hpy188I TCNGA 4 cut(s) 135, 205, 468, 853
Hpy188III TCNNGA 2 cut(s) 285, 453
Hpy8I GTNNAC 2 cut(s) 70, 814
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 4 cut(s) 150, 291, 374, 730
HpyCH4III ACNGT 2 cut(s) 313, 769
HpyF10VI GCNNNNNNNGC 2 cut(s) 731, 806
HpyF3I CTNAG 1 cut(s) 476
Hsp92II CATG 3 cut(s) 368, 504, 809
Kzo9I GATC 3 cut(s) 18, 644, 848
Lsp1109I GCAGC 2 cut(s) 244, 542
LweI GCATC 2 cut(s) 368, 445
MaeI CTAG 1 cut(s) 780
MaeIII GTNAC 2 cut(s) 313, 842
MalI GATC 3 cut(s) 20, 646, 850
MboI GATC 3 cut(s) 18, 644, 848
MboII GAAGA 6 cut(s) 37, 157, 218, 234, 431, 508
MflI RGATCY 1 cut(s) 644
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 8 cut(s) 89, 108, 136, 149, 250, 345, 649, 817
MlyI GAGTC 1 cut(s) 527
MmeI TCCRAC 1 cut(s) 805
MnlI CCTC 8 cut(s) 93, 256, 281, 421, 598, 648, 787, 821
MroXI GAANNNNTTC 1 cut(s) 715
MseI TTAA 2 cut(s) 219, 249
MspI CCGG 2 cut(s) 321, 506
MspR9I CCNGG 3 cut(s) 322, 594, 698
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 2 cut(s) 594, 698
MvnI CGCG 1 cut(s) 515
MwoI GCNNNNNNNGC 2 cut(s) 731, 806
NciI CCSGG 1 cut(s) 322
NdeII GATC 3 cut(s) 18, 644, 848
NlaIII CATG 3 cut(s) 368, 504, 809
NlaIV GGNNCC 2 cut(s) 7, 625
PctI GAATGC 1 cut(s) 275
PdmI GAANNNNTTC 1 cut(s) 715
PfeI GAWTC 2 cut(s) 239, 854
PflMI CCANNNNNTGG 1 cut(s) 641
PkrI GCNGC 3 cut(s) 259, 334, 532
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 544
Psp5II RGGWCCY 1 cut(s) 544
Psp6I CCWGG 2 cut(s) 592, 696
PspGI CCWGG 2 cut(s) 592, 696
PspN4I GGNNCC 2 cut(s) 7, 625
PspPI GGNCC 1 cut(s) 544
PspPPI RGGWCCY 1 cut(s) 544
PstI CTGCAG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 644
RsaI GTAC 1 cut(s) 414
RsaNI GTAC 1 cut(s) 413
SaqAI TTAA 2 cut(s) 219, 249
SatI GCNGC 3 cut(s) 258, 333, 531
Sau3AI GATC 3 cut(s) 18, 644, 848
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 3 cut(s) 322, 594, 698
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 368, 445
SfcI CTRYAG 1 cut(s) 258
SinI GGWCC 1 cut(s) 544
Sse9I AATT 8 cut(s) 89, 108, 136, 149, 250, 345, 649, 817
SsiI CCGC 2 cut(s) 332, 515
SspMI CTAG 1 cut(s) 780
StyD4I CCNGG 3 cut(s) 320, 592, 696
StyI CCWWGG 1 cut(s) 663
TaaI ACNGT 2 cut(s) 313, 769
TaqI TCGA 1 cut(s) 352
TasI AATT 8 cut(s) 89, 108, 136, 149, 250, 345, 649, 817
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 2 cut(s) 239, 854
Tru1I TTAA 2 cut(s) 219, 249
Tru9I TTAA 2 cut(s) 219, 249
TscAI CASTG 2 cut(s) 316, 709
TseI GCWGC 2 cut(s) 257, 530
TspDTI ATGAA 2 cut(s) 37, 846
TspRI CASTG 2 cut(s) 316, 709
Van91I CCANNNNNTGG 1 cut(s) 641
VneI GTGCAC 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 544
XagI CCTNNNNNAGG 1 cut(s) 285
XapI RAATTY 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 715
XspI CTAG 1 cut(s) 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.