MD13G1115600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013386
Physical Location & Seq
Forward (+)
8382400 .. 8383305
906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1115600.v1.1.491

Sequence Viewer

Length: 906 bp
ATGAAGTACCATTTTTCATGTACATGTCTGAATAAATTTGGAATTTCTCAGGAATCAACAGCACAAATCAACATGATCAGTCCCAGAAAACGCCCGTTGCACACTTGTGCGGTTTCATTTTTAGCGGTAGCTCATAGAGCCTTCACCATAGCCCAAGGTCTTAATGGACCGCTAGGGTTCATAATAAAAATTGCAGCCAGACTAATAACGTTGGCCGGACCCTTGGCTTATGCCTTGAAATACCAATGGGTCACAATGACAATCCTCTCATTCATCGACGGTCGAATTTTAGCCCTTGAAGGCATGATCGAGAGATTTTACCCTCCTTCACACATTGTGTTCGACAAGATCGACGACCTTGTCCAAGTCACCGAATCCCTGCCTGGAAAATTCGACAATGCTGTTAGCAAAATCCCTGCACATTTCCATCACATTTCATTTTTCGACTGGACGTTGGTTCGTGCCATTTGGCTGCTGAATTTTGTTGCGGCTACATTGACTAACTACTGGAGAAAGTCAAAGGGTACGAGGGAGAAGGAGATTAAGGTTGATACAAGCAGCAATATTGATCACAACTGTCAACCAGGTTCGGTTCATGAGGCCGACCAGTCTTCCCCAAATTGTAATAAGATTAATAAGGGTAATAATTTCTCTTCTCCGCGTAAAGCTGTTACTTACAAGGACGTGCTTCTACAGAAGGGAACAAATAAAGGAGGGATTGGAAAAAAAGAAGAGAACAAGGTTAACAACAGGGAAGAAGTGGAGAGTAGTGTTGCTGATGATGATGAAAGTGAAACCAACTCTAAGGATGATCTCATCGAAATGTTCGAATCATCCTGGCTTATGACATCGCCGGGAAAAAATGATGAAAATTACGCGCCGCGTTCTGTTTCATTCGCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

302

Amino Acids

33.77

Weight (kDa)

8.19

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013386)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67540 AT1G67540
fragaria_vesca FvH4_4g24090
malus_domestica MD13G1115600.v1.1 MD16G1115600.v1.1
prunus_persica Prupe.1G231500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0430861
rosa_laevigata RLG00000006945
rosa_multiflora Rmu_sc0002620.1_g000003 Rmu_sc0006263.1_g000006
rosa_roxburghii Rroxscaffold_5G00372530
rosa_rugosa Rorug04G0246000.1
rosa_samantha Rh4AG302300 Rh4BG310400 Rh4CG326700 Rh4CG326800 Rh4DG306500
rosa_wichuraiana Rw4G026270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AccII CGCG 3 cut(s) 661, 878, 883
AciI CCGC 6 cut(s) 110, 125, 170, 488, 659, 881
AclI AACGTT 1 cut(s) 209
AcoI YGGCCR 1 cut(s) 213
AcsI RAATTY 5 cut(s) 35, 42, 285, 389, 478
AdeI CACNNNGTG 1 cut(s) 337
AfaI GTAC 3 cut(s) 8, 22, 526
AflIII ACRYGT 1 cut(s) 23
AgsI TTSAA 2 cut(s) 238, 299
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 3 cut(s) 382, 583, 836
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 2 cut(s) 131, 668
AluI AGCT 2 cut(s) 131, 668
AoxI GGCC 2 cut(s) 213, 600
ApeKI GCWGC 3 cut(s) 194, 472, 558
ApoI RAATTY 5 cut(s) 35, 42, 285, 389, 478
AseI ATTAAT 1 cut(s) 633
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 2 cut(s) 167, 218
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 2 cut(s) 136, 361
AsuII TTCGAA 1 cut(s) 828
AvaII GGWCC 2 cut(s) 167, 218
BbsI GAAGAC 1 cut(s) 603
BbvI GCAGC 3 cut(s) 206, 459, 570
BccI CCATC 1 cut(s) 435
BciT130I CCWGG 3 cut(s) 384, 585, 838
BclI TGATCA 2 cut(s) 75, 568
BcnI CCSGG 1 cut(s) 855
BfaI CTAG 1 cut(s) 173
BfmI CTRYAG 1 cut(s) 692
BisI GCNGC 5 cut(s) 195, 473, 489, 559, 881
BlsI GCNGC 5 cut(s) 196, 474, 490, 560, 882
Bme1390I CCNGG 4 cut(s) 384, 585, 838, 855
Bme18I GGWCC 2 cut(s) 167, 218
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 2 cut(s) 167, 218
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 4 cut(s) 384, 585, 838, 855
BpiI GAAGAC 1 cut(s) 603
BpmI CTGGAG 1 cut(s) 529
Bpu14I TTCGAA 1 cut(s) 828
BpuMI CCSGG 1 cut(s) 855
BsaJI CCNNGG 2 cut(s) 154, 222
Bse1I ACTGG 3 cut(s) 452, 512, 607
BseBI CCWGG 3 cut(s) 384, 585, 838
BseDI CCNNGG 2 cut(s) 154, 222
BseGI GGATG 2 cut(s) 814, 833
BseMII CTCAG 1 cut(s) 62
BseNI ACTGG 3 cut(s) 452, 512, 607
BseXI GCAGC 3 cut(s) 206, 459, 570
BsgI GTGCAG 1 cut(s) 402
Bsh1236I CGCG 3 cut(s) 661, 878, 883
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 215, 602
BsiEI CGRYCG 1 cut(s) 283
BsiSI CCGG 2 cut(s) 216, 854
BslFI GGGAC 1 cut(s) 66
BsmFI GGGAC 1 cut(s) 66
BsnI GGCC 2 cut(s) 215, 602
Bsp119I TTCGAA 1 cut(s) 828
Bsp1407I TGTACA 1 cut(s) 20
Bsp143I GATC 5 cut(s) 75, 306, 348, 568, 811
BspACI CCGC 6 cut(s) 110, 125, 170, 488, 659, 881
BspANI GGCC 2 cut(s) 215, 602
BspCNI CTCAG 1 cut(s) 61
BspFNI CGCG 3 cut(s) 661, 878, 883
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 828
BsrGI TGTACA 1 cut(s) 20
BsrI ACTGG 3 cut(s) 452, 512, 607
BssECI CCNNGG 2 cut(s) 154, 222
BssMI GATC 5 cut(s) 75, 306, 348, 568, 811
BssT1I CCWWGG 2 cut(s) 154, 222
Bst2UI CCWGG 3 cut(s) 384, 585, 838
Bst4CI ACNGT 2 cut(s) 281, 578
Bst6I CTCTTC 2 cut(s) 658, 726
BstAUI TGTACA 1 cut(s) 20
BstBI TTCGAA 1 cut(s) 828
BstDEI CTNAG 2 cut(s) 48, 804
BstF5I GGATG 2 cut(s) 814, 833
BstFNI CGCG 3 cut(s) 661, 878, 883
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 5 cut(s) 78, 309, 351, 571, 814
BstMBI GATC 5 cut(s) 75, 306, 348, 568, 811
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNI CCWGG 3 cut(s) 384, 585, 838
BstNSI RCATGY 1 cut(s) 27
BstSCI CCNGG 4 cut(s) 382, 583, 836, 853
BstSFI CTRYAG 1 cut(s) 692
BstUI CGCG 3 cut(s) 661, 878, 883
BstV1I GCAGC 3 cut(s) 206, 459, 570
BstV2I GAAGAC 1 cut(s) 603
BsuRI GGCC 2 cut(s) 215, 602
BtgZI GCGATG 1 cut(s) 834
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 2 cut(s) 814, 833
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 880
Cfr13I GGNCC 2 cut(s) 167, 218
CsiI ACCWGGT 1 cut(s) 583
Csp6I GTAC 3 cut(s) 7, 21, 525
CviAII CATG 5 cut(s) 18, 24, 73, 304, 596
CviQI GTAC 3 cut(s) 7, 21, 525
DdeI CTNAG 2 cut(s) 48, 804
DpnI GATC 5 cut(s) 77, 308, 350, 570, 813
DpnII GATC 5 cut(s) 75, 306, 348, 568, 811
DraIII CACNNNGTG 1 cut(s) 337
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 1 cut(s) 213
Eam1104I CTCTTC 2 cut(s) 658, 726
EarI CTCTTC 2 cut(s) 658, 726
Eco130I CCWWGG 2 cut(s) 154, 222
Eco47I GGWCC 2 cut(s) 167, 218
EcoRII CCWGG 3 cut(s) 382, 583, 836
EcoT14I CCWWGG 2 cut(s) 154, 222
ErhI CCWWGG 2 cut(s) 154, 222
FaeI CATG 5 cut(s) 21, 27, 76, 307, 599
FaqI GGGAC 1 cut(s) 66
FatI CATG 5 cut(s) 17, 23, 72, 303, 595
FbaI TGATCA 2 cut(s) 75, 568
Fnu4HI GCNGC 5 cut(s) 195, 473, 489, 559, 881
FokI GGATG 2 cut(s) 820, 821
Fsp4HI GCNGC 5 cut(s) 195, 473, 489, 559, 881
FspBI CTAG 1 cut(s) 173
GlaI GCGC 1 cut(s) 879
GluI GCNGC 5 cut(s) 195, 473, 489, 559, 881
GsuI CTGGAG 1 cut(s) 529
HaeIII GGCC 2 cut(s) 215, 602
HapII CCGG 2 cut(s) 216, 854
HhaI GCGC 1 cut(s) 880
Hin1II CATG 5 cut(s) 21, 27, 76, 307, 599
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HincII GTYRAC 2 cut(s) 581, 745
HindII GTYRAC 2 cut(s) 581, 745
HinfI GANTC 3 cut(s) 53, 374, 830
HpaI GTTAAC 1 cut(s) 745
HpaII CCGG 2 cut(s) 216, 854
HphI GGTGA 2 cut(s) 136, 361
Hpy166II GTNNAC 2 cut(s) 581, 745
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 3 cut(s) 50, 310, 596
Hpy8I GTNNAC 2 cut(s) 581, 745
Hpy99I CGWCG 2 cut(s) 281, 356
HpyAV CCTTC 5 cut(s) 151, 293, 336, 529, 691
HpyCH4III ACNGT 2 cut(s) 281, 578
HpyCH4IV ACGT 3 cut(s) 209, 452, 684
HpyCH4V TGCA 3 cut(s) 100, 194, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 2 cut(s) 48, 804
HpySE526I ACGT 3 cut(s) 209, 452, 684
Hsp92II CATG 5 cut(s) 21, 27, 76, 307, 599
HspAI GCGC 1 cut(s) 878
Ksp22I TGATCA 2 cut(s) 75, 568
KspAI GTTAAC 1 cut(s) 745
Kzo9I GATC 5 cut(s) 75, 306, 348, 568, 811
Lsp1109I GCAGC 3 cut(s) 206, 459, 570
MabI ACCWGGT 1 cut(s) 583
MaeI CTAG 1 cut(s) 173
MaeII ACGT 3 cut(s) 209, 452, 684
MaeIII GTNAC 3 cut(s) 250, 367, 670
MalI GATC 5 cut(s) 77, 308, 350, 570, 813
MboI GATC 5 cut(s) 75, 306, 348, 568, 811
MboII GAAGA 4 cut(s) 603, 645, 743, 767
MluCI AATT 9 cut(s) 35, 42, 189, 285, 389, 478, 619, 646, 871
MnlI CCTC 5 cut(s) 275, 333, 522, 592, 707
MseI TTAA 5 cut(s) 162, 543, 633, 744, 904
MslI CAYNNNNRTG 3 cut(s) 22, 105, 821
MspI CCGG 2 cut(s) 216, 854
MspR9I CCNGG 4 cut(s) 384, 585, 838, 855
MvaI CCWGG 3 cut(s) 384, 585, 838
MvnI CGCG 3 cut(s) 661, 878, 883
MwoI GCNNNNNNNGC 1 cut(s) 137
NciI CCSGG 1 cut(s) 855
NdeII GATC 5 cut(s) 75, 306, 348, 568, 811
NlaIII CATG 5 cut(s) 21, 27, 76, 307, 599
NlaIV GGNNCC 1 cut(s) 220
NmuCI GTSAC 2 cut(s) 250, 367
NspI RCATGY 1 cut(s) 27
NspV TTCGAA 1 cut(s) 828
OliI CACNNNNGTG 1 cut(s) 105
PagI TCATGA 1 cut(s) 595
PciI ACATGT 1 cut(s) 23
PcsI WCGNNNNNNNCGW 2 cut(s) 348, 825
PfeI GAWTC 3 cut(s) 53, 374, 830
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 5 cut(s) 196, 474, 490, 560, 882
PscI ACATGT 1 cut(s) 23
PshBI ATTAAT 1 cut(s) 633
Psp1406I AACGTT 1 cut(s) 209
Psp6I CCWGG 3 cut(s) 382, 583, 836
PspGI CCWGG 3 cut(s) 382, 583, 836
PspN4I GGNNCC 1 cut(s) 220
PspPI GGNCC 2 cut(s) 167, 218
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 3 cut(s) 8, 22, 526
RsaNI GTAC 3 cut(s) 7, 21, 525
RseI CAYNNNNRTG 3 cut(s) 22, 105, 821
SaqAI TTAA 5 cut(s) 162, 543, 633, 744, 904
SatI GCNGC 5 cut(s) 195, 473, 489, 559, 881
Sau3AI GATC 5 cut(s) 75, 306, 348, 568, 811
Sau96I GGNCC 2 cut(s) 167, 218
ScrFI CCNGG 4 cut(s) 384, 585, 838, 855
SexAI ACCWGGT 1 cut(s) 583
SfcI CTRYAG 1 cut(s) 692
SfuI TTCGAA 1 cut(s) 828
SinI GGWCC 2 cut(s) 167, 218
SmiMI CAYNNNNRTG 3 cut(s) 22, 105, 821
Sse9I AATT 9 cut(s) 35, 42, 189, 285, 389, 478, 619, 646, 871
SsiI CCGC 6 cut(s) 110, 125, 170, 488, 659, 881
SspI AATATT 1 cut(s) 565
SspMI CTAG 1 cut(s) 173
StyD4I CCNGG 4 cut(s) 382, 583, 836, 853
StyI CCWWGG 2 cut(s) 154, 222
TaaI ACNGT 2 cut(s) 281, 578
TaiI ACGT 3 cut(s) 212, 455, 687
TaqI TCGA 9 cut(s) 276, 283, 309, 342, 351, 393, 444, 819, 828
TasI AATT 9 cut(s) 35, 42, 189, 285, 389, 478, 619, 646, 871
TatI WGTACW 1 cut(s) 20
TauI GCSGC 2 cut(s) 491, 883
TfiI GAWTC 3 cut(s) 53, 374, 830
Tru1I TTAA 5 cut(s) 162, 543, 633, 744, 904
Tru9I TTAA 5 cut(s) 162, 543, 633, 744, 904
TseFI GTSAC 2 cut(s) 250, 367
TseI GCWGC 3 cut(s) 194, 472, 558
Tsp45I GTSAC 2 cut(s) 250, 367
Tth111I GACNNNGTC 1 cut(s) 359
VpaK11BI GGWCC 2 cut(s) 167, 218
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 5 cut(s) 35, 42, 285, 389, 478
XceI RCATGY 1 cut(s) 27
XcmI CCANNNNNNNNNTGG 1 cut(s) 161
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.