MD16G1115600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013386
Physical Location & Seq
Forward (+)
8233499 .. 8234356
858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1115600.v1.1.491

Sequence Viewer

Length: 858 bp
ATGATCAGTCCCAGAAAACGCCCGTTGCACACTTGTGCGGTTTCAATTTTACTGGTAGCTCATAGAGCCTTCACCATAGCCCAAGGTCTCAATGGACCACTAGGGTTCATAATAAAAATTGCAGCCAGACTAGTCACGTTGGCCGGAACCTTGGCTTATGCCTTGCAATACCAATGGCTCACACTGGCAATCCTCTCATTCATCGATGATCGAATTTTAGCCCTTGAAGACATGATCGAGAGATTTTACCCTCCTTCACACTTTGTGTTCGACAAGATTGACGACCTTGTCCAAGTCACCGAATCCCTTCCTGGAAAATTTGACAATGCCGTAGGCAAAATCCTGGCACATTTCCATCACGTTTCGTTTTTGGATTGGACGTTGGTGCGTGCCATTTCGCTGCTGAATTTTGTTGCGGCTACATTGAACTATTGGAGAATGTCCAACTGTACAAGGGAGAAGGAGATTAAGGTTGATGCAAGCAGGAATATTGATCGCAACCGTGGACCGTGTTCGGTTCATGAAGCTGACTGGTCTACCCCAAATGGTAACAAGAATAATGCTGCAGCAAGTGGTGATAAGATTAATAAGGGTAATACCTATTCTTCTCCTCGTAGAGGTGTTACTTACAAGGAAGTGCTTCTGCAGAAGGGAACAAATAATGGAGGGATTGGAAAAAAAGAAGAGAATAAGGTTAATAACAAGGAAGAAGTGGAGAGTAGTGTTGCTGATGATGATAGTGAAACCAACTCTAAAGATGATGACCTCCTTGAATTGTTTGAATCAGCGTGGCTTATGACATCGCCTGGAAAAAATAAGGAAAAATACGCGCCACGTTCAGTTTCATTTATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

31.87

Weight (kDa)

7.72

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013386)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67540 AT1G67540
fragaria_vesca FvH4_4g24090
malus_domestica MD13G1115600.v1.1 MD16G1115600.v1.1
prunus_persica Prupe.1G231500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0430861
rosa_laevigata RLG00000006945
rosa_multiflora Rmu_sc0002620.1_g000003 Rmu_sc0006263.1_g000006
rosa_roxburghii Rroxscaffold_5G00372530
rosa_rugosa Rorug04G0246000.1
rosa_samantha Rh4AG302300 Rh4BG310400 Rh4CG326700 Rh4CG326800 Rh4DG306500
rosa_wichuraiana Rw4G026270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 536
AccII CGCG 1 cut(s) 830
AciI CCGC 2 cut(s) 38, 416
AcoI YGGCCR 1 cut(s) 141
AcsI RAATTY 3 cut(s) 213, 317, 406
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 1 cut(s) 617
AgsI TTSAA 5 cut(s) 45, 227, 427, 773, 782
AhlI ACTAGT 1 cut(s) 130
AjnI CCWGG 3 cut(s) 310, 342, 805
AleI CACNNNNGTG 1 cut(s) 33
AluBI AGCT 2 cut(s) 59, 527
AluI AGCT 2 cut(s) 59, 527
Alw26I GTCTC 1 cut(s) 92
AoxI GGCC 1 cut(s) 141
ApeKI GCWGC 4 cut(s) 122, 400, 563, 566
ApoI RAATTY 3 cut(s) 213, 317, 406
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 2 cut(s) 306, 639
AspLEI GCGC 1 cut(s) 832
AspS9I GGNCC 2 cut(s) 95, 506
AsuHPI GGTGA 3 cut(s) 64, 289, 587
AvaII GGWCC 2 cut(s) 95, 506
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 134, 387, 550, 578
BccI CCATC 1 cut(s) 363
BceAI ACGGC 1 cut(s) 314
BciT130I CCWGG 3 cut(s) 312, 344, 807
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 92
BcuI ACTAGT 1 cut(s) 130
BfaI CTAG 2 cut(s) 101, 131
BfmI CTRYAG 2 cut(s) 564, 644
BisI GCNGC 5 cut(s) 123, 401, 417, 564, 567
BlsI GCNGC 5 cut(s) 124, 402, 418, 565, 568
Bme1390I CCNGG 3 cut(s) 312, 344, 807
Bme18I GGWCC 2 cut(s) 95, 506
BmgT120I GGNCC 2 cut(s) 95, 506
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 3 cut(s) 312, 344, 807
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 82, 150, 502
Bsc4I CCNNNNNNNGG 1 cut(s) 617
Bse1I ACTGG 3 cut(s) 57, 189, 536
BseBI CCWGG 3 cut(s) 312, 344, 807
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 3 cut(s) 82, 150, 502
BseLI CCNNNNNNNGG 1 cut(s) 617
BseNI ACTGG 3 cut(s) 57, 189, 536
BseRI GAGGAG 1 cut(s) 600
BseXI GCAGC 4 cut(s) 134, 387, 550, 578
Bsh1236I CGCG 1 cut(s) 830
BshFI GGCC 1 cut(s) 143
BshVI ATCGAT 1 cut(s) 204
BsiSI CCGG 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 617
BsmAI GTCTC 1 cut(s) 92
BsnI GGCC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 92
Bsp1407I TGTACA 1 cut(s) 449
Bsp143I GATC 4 cut(s) 3, 208, 234, 493
BspACI CCGC 2 cut(s) 38, 416
BspANI GGCC 1 cut(s) 143
BspDI ATCGAT 1 cut(s) 204
BspFNI CGCG 1 cut(s) 830
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 1 cut(s) 148
BspMAI CTGCAG 2 cut(s) 568, 648
BspTNI GGTCTC 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 449
BsrI ACTGG 3 cut(s) 57, 189, 536
BssECI CCNNGG 3 cut(s) 82, 150, 502
BssMI GATC 4 cut(s) 3, 208, 234, 493
BssT1I CCWWGG 2 cut(s) 82, 150
Bst2UI CCWGG 3 cut(s) 312, 344, 807
Bst4CI ACNGT 3 cut(s) 449, 503, 510
Bst6I CTCTTC 1 cut(s) 678
BstAUI TGTACA 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 390, 481
BstDSI CCRYGG 1 cut(s) 502
BstENI CCTNNNNNAGG 1 cut(s) 615
BstFNI CGCG 1 cut(s) 830
BstHHI GCGC 1 cut(s) 832
BstKTI GATC 4 cut(s) 6, 211, 237, 496
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 4 cut(s) 3, 208, 234, 493
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNI CCWGG 3 cut(s) 312, 344, 807
BstSCI CCNGG 3 cut(s) 310, 342, 805
BstSFI CTRYAG 2 cut(s) 564, 644
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 4 cut(s) 134, 387, 550, 578
BstV2I GAAGAC 1 cut(s) 234
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 1 cut(s) 143
BsuTUI ATCGAT 1 cut(s) 204
BtgI CCRYGG 1 cut(s) 502
BtgZI GCGATG 1 cut(s) 786
BtsIMutI CAGTG 1 cut(s) 182
Cac8I GCNNGC 2 cut(s) 390, 481
CciI TCATGA 1 cut(s) 520
CfoI GCGC 1 cut(s) 832
Cfr13I GGNCC 2 cut(s) 95, 506
ClaI ATCGAT 1 cut(s) 204
Csp6I GTAC 1 cut(s) 450
CviAII CATG 2 cut(s) 232, 521
CviQI GTAC 1 cut(s) 450
DpnI GATC 4 cut(s) 5, 210, 236, 495
DpnII GATC 4 cut(s) 3, 208, 234, 493
DraIII CACNNNGTG 1 cut(s) 265
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 141
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Eco130I CCWWGG 2 cut(s) 82, 150
Eco31I GGTCTC 1 cut(s) 92
Eco47I GGWCC 2 cut(s) 95, 506
EcoNI CCTNNNNNAGG 1 cut(s) 615
EcoRII CCWGG 3 cut(s) 310, 342, 805
EcoT14I CCWWGG 2 cut(s) 82, 150
ErhI CCWWGG 2 cut(s) 82, 150
FaeI CATG 2 cut(s) 235, 524
FaiI YATR 7 cut(s) 63, 77, 110, 159, 233, 522, 797
FatI CATG 2 cut(s) 231, 520
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 536
Fnu4HI GCNGC 5 cut(s) 123, 401, 417, 564, 567
Fsp4HI GCNGC 5 cut(s) 123, 401, 417, 564, 567
FspBI CTAG 2 cut(s) 101, 131
GlaI GCGC 1 cut(s) 831
GluI GCNGC 5 cut(s) 123, 401, 417, 564, 567
HaeIII GGCC 1 cut(s) 143
HapII CCGG 1 cut(s) 144
HhaI GCGC 1 cut(s) 832
Hin1II CATG 2 cut(s) 235, 524
Hin6I GCGC 1 cut(s) 830
HinP1I GCGC 1 cut(s) 830
HinfI GANTC 2 cut(s) 302, 782
HpaII CCGG 1 cut(s) 144
HphI GGTGA 3 cut(s) 64, 289, 587
Hpy166II GTNNAC 2 cut(s) 506, 537
Hpy188III TCNNGA 2 cut(s) 238, 521
Hpy8I GTNNAC 2 cut(s) 506, 537
HpyAV CCTTC 5 cut(s) 79, 264, 317, 454, 643
HpyCH4III ACNGT 3 cut(s) 449, 503, 510
HpyCH4IV ACGT 4 cut(s) 137, 360, 380, 835
HpyCH4V TGCA 6 cut(s) 28, 122, 166, 479, 566, 646
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpySE526I ACGT 4 cut(s) 137, 360, 380, 835
Hsp92II CATG 2 cut(s) 235, 524
HspAI GCGC 1 cut(s) 830
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 208, 234, 493
Lsp1109I GCAGC 4 cut(s) 134, 387, 550, 578
LweI GCATC 1 cut(s) 466
MaeI CTAG 2 cut(s) 101, 131
MaeII ACGT 4 cut(s) 137, 360, 380, 835
MaeIII GTNAC 4 cut(s) 133, 295, 548, 622
MalI GATC 4 cut(s) 5, 210, 236, 495
MboI GATC 4 cut(s) 3, 208, 234, 493
MboII GAAGA 4 cut(s) 239, 597, 695, 719
MluCI AATT 6 cut(s) 45, 117, 213, 317, 406, 773
MmeI TCCRAC 1 cut(s) 468
MnlI CCTC 6 cut(s) 203, 261, 611, 621, 659, 776
MroXI GAANNNNTTC 2 cut(s) 306, 639
MseI TTAA 3 cut(s) 468, 585, 696
MslI CAYNNNNRTG 1 cut(s) 33
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 3 cut(s) 312, 344, 807
MvaI CCWGG 3 cut(s) 312, 344, 807
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 1 cut(s) 65
NdeII GATC 4 cut(s) 3, 208, 234, 493
NlaIII CATG 2 cut(s) 235, 524
NlaIV GGNNCC 1 cut(s) 148
NmuCI GTSAC 2 cut(s) 133, 295
OliI CACNNNNGTG 1 cut(s) 33
PagI TCATGA 1 cut(s) 520
PdmI GAANNNNTTC 2 cut(s) 306, 639
PfeI GAWTC 2 cut(s) 302, 782
PflFI GACNNNGTC 1 cut(s) 287
PfoI TCCNGGA 1 cut(s) 310
PkrI GCNGC 5 cut(s) 124, 402, 418, 565, 568
PshBI ATTAAT 1 cut(s) 585
Psp6I CCWGG 3 cut(s) 310, 342, 805
PspGI CCWGG 3 cut(s) 310, 342, 805
PspN4I GGNNCC 1 cut(s) 148
PspPI GGNCC 2 cut(s) 95, 506
PstI CTGCAG 2 cut(s) 568, 648
PsyI GACNNNGTC 1 cut(s) 287
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 3 cut(s) 468, 585, 696
SatI GCNGC 5 cut(s) 123, 401, 417, 564, 567
Sau3AI GATC 4 cut(s) 3, 208, 234, 493
Sau96I GGNCC 2 cut(s) 95, 506
ScrFI CCNGG 3 cut(s) 312, 344, 807
SfaNI GCATC 1 cut(s) 466
SfcI CTRYAG 2 cut(s) 564, 644
SinI GGWCC 2 cut(s) 95, 506
SmiMI CAYNNNNRTG 1 cut(s) 33
SpeI ACTAGT 1 cut(s) 130
Sse9I AATT 6 cut(s) 45, 117, 213, 317, 406, 773
SsiI CCGC 2 cut(s) 38, 416
SspI AATATT 1 cut(s) 490
SspMI CTAG 2 cut(s) 101, 131
StyD4I CCNGG 3 cut(s) 310, 342, 805
StyI CCWWGG 2 cut(s) 82, 150
TaaI ACNGT 3 cut(s) 449, 503, 510
TaiI ACGT 4 cut(s) 140, 363, 383, 838
TaqI TCGA 4 cut(s) 204, 211, 237, 270
TasI AATT 6 cut(s) 45, 117, 213, 317, 406, 773
TatI WGTACW 1 cut(s) 449
TauI GCSGC 1 cut(s) 419
TfiI GAWTC 2 cut(s) 302, 782
Tru1I TTAA 3 cut(s) 468, 585, 696
Tru9I TTAA 3 cut(s) 468, 585, 696
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 2 cut(s) 133, 295
TseI GCWGC 4 cut(s) 122, 400, 563, 566
Tsp45I GTSAC 2 cut(s) 133, 295
TspDTI ATGAA 5 cut(s) 97, 190, 509, 537, 834
TspRI CASTG 1 cut(s) 189
Tth111I GACNNNGTC 1 cut(s) 287
VpaK11BI GGWCC 2 cut(s) 95, 506
VspI ATTAAT 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 615
XapI RAATTY 3 cut(s) 213, 317, 406
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 536
XmnI GAANNNNTTC 2 cut(s) 306, 639
XspI CTAG 2 cut(s) 101, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.