Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013838

Physical Location & Seq

Genomic Coordinates Forward (+)

9189559 .. 9196170

Gene Structure 6612 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1123700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGGCCTCCAGTGCCGTTAACGTCAAGCAAGAACCCTCCGAGAAACCCTACCAAATCCCCAACCTTCTGCCAATCCCATTAAGCAGCGATAGTAGCAGTAGCAGTAGCAGTAGCGCCAGTTCCGTCAATTCTTCCGACATCGAAGGCCTTGAGACCAATTCCTTGTTCGATTCCGTGACGAGTAAGTTGAACGGCGACGATCGCGATTTGGCGGATGGAGGGGATGATGCTCCGGCGGCGAAGAAGGCCAAACGCGACGGTGAAAGGGGGTTCGTGTTGCCGCTAGGGTTTCTGCGTCCCCTCCCGCCGTTGGAATTGTCCAAGGAGTTGCCCCTGGCTCTGGTTTTGCCCGACTCGACAATGTGCTTGCCGAGTGGGTCCGGTTGTAATAACCAGGTGGGCCCGGTTATAAGGGGATGCAAGCAGTTTTGGAAAGCAGGAGATTATGAGGGTGACAGTGGAGGCATTGCCATTTCTGGGTTTTCGTCTGCTGGCATGGACCATGTTAGGGTTCATCCCAAGTTTCTCCATTCAAATGCAACCAGCCATAAGTGGGCTCTGGGAGCTTTTGCGGAGCTCCTAGACAATGCTTTAGATGAGGTCTGCAATGGGGCCAACAATGTTTGTGTTGACGTGGTGAGAAACAAAAAAGATGGTAATGAGATGTTGATGGTTGAAGATGACGGTGGTGGGATGACGCCTGAAAAAATGCGAGAATGCATGTCTCTTGGGTATTCTGCAAAAAGCAAATTGGCAAATACTATTGGTCAATATGGCAATGGGTTTAAGACTAGTACTATGAGGCTTGGAGCAGATGTAATAGTGTTTTCTCGTTGTGAGGGGGAGGATGGGAGAAGACCGACACAGACCATTGGAGTGCTATCCTACACCTTTTTGAGGGGTACTGGAAAGGAAGATATTGTGGTTCCCATGATTGATTATGAGAAAAGAGGCCAAGGTTGGAACAAGATGATGCGGGGTTCTCCAGATGACTGGCATAGGAACTTAGCAACCATAGTGCAGTGGTCTCCTTATTTAAGCGAAGAAGATCTTCTTCAGCAGTTTAATCTCTTTAAAGAGCATGGAACACGTATAATAATATACAACCTTTGGGAGGATGATGAAGGAAAACTGGAACTTGATTTCGACACTGATCCTCATGATATTCAAATTAGAGGAGTCAATAGGGATGAGAAGAACATTCAGATGGCAAAACGGTATCCCAACTCTAGACACTTCTTAACCTATCGGCATTCATTAAGGAGTTATGCATCAATTCTTTATCTCAGGCTTCCATACAACTTCAGAATTATTCTTCGTGGGAAAGATGTTGAACATCATAATATAGTTAATGATATGATGTTAACGAAGGAGGTAACATACAGACCTCTGCAACTTCCGGAAGGAATGTCGCCAAAAGTTGCAAATATGACTGCAAATGTGACACTTGGTTTTGTAAAGGATGCAAAAGATCATATTGCTGTCCAAGGGTTCAGCGTTTATCATAAAAATAGACTGATTAAGCCGTTTTGGAGGCTGTGGAATCCTGCTGGAAGTCAAGGTCGTGGCATTATAGGTGTTTTGGAAGCTGATTTTGTTGAACCAACTCACGACAAACAGGGCTTTGAGCGGACTGCTGTGCTTTCAAGACTAGAGAATAAATTGGTTAAAATTCAAAAGGATTACTGGTATGTAAACTGCCAGACTGTTGGGTATGCTCCAGTAAGACGTACTACTGATTTTCCAGAAAGAGAACCTTCGGTGTCTGTTAGAAGGAAATCCTGTCGCGTAAATCAGAATTGCCATGGTTCTAGTAGTGGACGACTTAAGTCCAGTAACAAACTTGAAAAAGGATCATTAGCCATAAATGTTTCAAGTGAAAATGGGAGCTGTCATGAGGCATCACATTCTTGTATTGAACCTCAGCAAGATCCTGCTTCAGATGAAAGAGTCCCTCAAATGCTTACGCGTTCACAGTCAAAGACGGTTGCTACAAACAGAGTGGGACAAGTTTCTACAGATTGCGATGCTGAGTGTTCAACCAAGTCGAAAAAGGAATCCCATGAAGCGACAGAAAGTTTGAGGGAAATTGTCAGTCCAGCAATGGCTAAATTGCTAAACGAGCGCAGTTGTGAAAGGTGCAAATCTCTTGGGACTGAACTTGAGCAGGCAAAAGAAAAAATAGAAGAGTTGGAGAAAGAGCAATCGGCTTTCCTAGAAATAATAGTGCAAGAAAAGGCCGAGCGAGAACAAGAGGAGGAAAGATTAAGGAAAAGCCTAACGGATGCTTCTAACACGATCGGCAAGTTGCTTGAGAAGGTGAAAGAGCTAGAAGGAAGGAAGCTACAGAACCCTAAAGTTGAGCATGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006282 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031935 GO:0033043 GO:0033044 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048583 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051252 GO:0060255 GO:0060968 GO:0065007 GO:0071704 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:1902275 GO:1903506 GO:2000112 GO:2001020 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HATPase_c_3

Protein Analysis

791

Amino Acids

88.18

Weight (kDa)

6.23

Isoelectric Point (pI)

48.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HATPase_c_3	PF13589	188 - 283	9.7e-17	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HATPase_c	PF02518	189 - 268	8.1e-06	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Morc6_S5	PF17942	418 - 563	2.4e-57	Morc6 ribosomal protein S5 domain 2-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013838)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13130 AT5G13130
fragaria_vesca	FvH4_4g23220
malus_domestica	MD13G1123700.v1.1
prunus_persica	Prupe.1G221200_v2.0.a1
pyrus_communis	pycom13g10820
rosa_chinensis	RchiOBHm_Chr4g0429571
rosa_laevigata	RLG00000007060
rosa_multiflora	Rmu_co8379671.1_g000001
rosa_roxburghii	Rroxscaffold_5G00371270
rosa_rugosa	Rorug04G0237200 Rorug04G0237300
rosa_samantha	Rh4AG293000 Rh4BG298700 Rh4CG314000 Rh4DG295500
rosa_wichuraiana	Rw4G025360

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	410
AccBSI	CCGCTC	1 cut(s)	1630
AccII	CGCG	4 cut(s)	204, 255, 1788, 1969
AccIII	TCCGGA	1 cut(s)	1399
AciI	CCGC	7 cut(s)	212, 236, 281, 305, 572, 976, 1630
AclWI	GGATC	3 cut(s)	1148, 1861, 1925
AcsI	RAATTY	1 cut(s)	1671
AcuI	CTGAAG	3 cut(s)	1040, 1288, 1923
AcyI	GRCGYC	1 cut(s)	698
AfaI	GTAC	3 cut(s)	796, 904, 1732
AfiI	CCNNNNNNNGG	7 cut(s)	310, 340, 477, 508, 553, 897, 1114
AflII	CTTAAG	1 cut(s)	1826
AflIII	ACRYGT	2 cut(s)	1088, 1967
AhlI	ACTAGT	1 cut(s)	791
AjiI	CACGTC	1 cut(s)	634
AjnI	CCWGG	2 cut(s)	333, 393
AjuI	GAANNNNNNNTTGG	2 cut(s)	149, 181
AluBI	AGCT	6 cut(s)	566, 577, 1589, 1890, 2329, 2344
AluI	AGCT	6 cut(s)	566, 577, 1589, 1890, 2329, 2344
Alw21I	GWGCWC	1 cut(s)	579
Alw26I	GTCTC	3 cut(s)	146, 729, 1032
AlwI	GGATC	3 cut(s)	1148, 1861, 1925
Aor13HI	TCCGGA	1 cut(s)	1399
AoxI	GGCC	7 cut(s)	3, 145, 246, 400, 612, 952, 2238
ApaI	GGGCCC	1 cut(s)	404
ApeKI	GCWGC	1 cut(s)	84
ApoI	RAATTY	1 cut(s)	1671
ArsI	GACNNNNNNTTYG	2 cut(s)	2062, 2094
Asp700I	GAANNNNTTC	1 cut(s)	1050
AspLEI	GCGC	2 cut(s)	116, 2127
AspS9I	GGNCC	5 cut(s)	378, 400, 401, 499, 612
AsuC2I	CCSGG	1 cut(s)	404
AsuHPI	GGTGA	4 cut(s)	272, 464, 649, 2332
AvaII	GGWCC	2 cut(s)	378, 499
BaeGI	GKGCMC	1 cut(s)	404
BanII	GRGCYC	3 cut(s)	404, 559, 579
BbsI	GAAGAC	1 cut(s)	862
Bbv12I	GWGCWC	1 cut(s)	579
BbvCI	CCTCAGC	1 cut(s)	1923
BbvI	GCAGC	1 cut(s)	96
BccI	CCATC	5 cut(s)	209, 647, 664, 842, 1201
BceAI	ACGGC	3 cut(s)	208, 292, 1510
BciT130I	CCWGG	2 cut(s)	335, 395
BciVI	GTATCC	1 cut(s)	1230
BcnI	CCSGG	1 cut(s)	404
BcoDI	GTCTC	3 cut(s)	146, 729, 1032
BcuI	ACTAGT	1 cut(s)	791
BfaI	CTAG	8 cut(s)	284, 581, 792, 1230, 1652, 1812, 2216, 2330
BfmI	CTRYAG	2 cut(s)	2016, 2345
BfoI	RGCGCY	1 cut(s)	117
BfrI	CTTAAG	1 cut(s)	1826
BfuI	GTATCC	1 cut(s)	1230
BglII	AGATCT	1 cut(s)	1048
BisI	GCNGC	3 cut(s)	85, 237, 281
BlsI	GCNGC	3 cut(s)	86, 238, 282
BmcAI	AGTACT	1 cut(s)	796
Bme1390I	CCNGG	3 cut(s)	335, 395, 404
Bme18I	GGWCC	2 cut(s)	378, 499
BmgBI	CACGTC	1 cut(s)	634
BmgT120I	GGNCC	5 cut(s)	378, 400, 401, 499, 612
BmiI	GGNNCC	4 cut(s)	379, 402, 613, 927
BmrFI	CCNGG	3 cut(s)	335, 395, 404
BmsI	GCATC	8 cut(s)	217, 407, 963, 1280, 1453, 1910, 2017, 2275
BoxI	GACNNNNGTC	1 cut(s)	1828
BpiI	GAAGAC	1 cut(s)	862
BpmI	CTGGAG	2 cut(s)	969, 1704
Bpu10I	CCTNAGC	1 cut(s)	1923
BpuEI	CTTGAG	3 cut(s)	170, 2183, 2333
BpuMI	CCSGG	1 cut(s)	404
BsaAI	YACGTR	1 cut(s)	1091
BsaHI	GRCGYC	1 cut(s)	698
BsaI	GGTCTC	2 cut(s)	146, 1032
BsaJI	CCNNGG	5 cut(s)	321, 333, 955, 1486, 1804
BsaWI	WCCGGW	2 cut(s)	380, 1399
BsaXI	ACNNNNNCTCC	2 cut(s)	801, 831
Bsc4I	CCNNNNNNNGG	7 cut(s)	310, 340, 477, 508, 553, 897, 1114
Bse1I	ACTGG	8 cut(s)	9, 117, 910, 998, 1137, 1691, 1721, 1833
Bse3DI	GCAATG	4 cut(s)	465, 613, 784, 2109
BseAI	TCCGGA	1 cut(s)	1399
BseBI	CCWGG	2 cut(s)	335, 395
BseDI	CCNNGG	5 cut(s)	321, 333, 955, 1486, 1804
BseLI	CCNNNNNNNGG	7 cut(s)	310, 340, 477, 508, 553, 897, 1114
BseMI	GCAATG	4 cut(s)	465, 613, 784, 2109
BseMII	CTCAG	3 cut(s)	1300, 1937, 2022
BseNI	ACTGG	8 cut(s)	9, 117, 910, 998, 1137, 1691, 1721, 1833
BseRI	GAGGAG	2 cut(s)	1191, 2270
BseSI	GKGCMC	1 cut(s)	404
BseXI	GCAGC	1 cut(s)	96
BsgI	GTGCAG	1 cut(s)	1040
Bsh1236I	CGCG	4 cut(s)	204, 255, 1788, 1969
Bsh1285I	CGRYCG	2 cut(s)	202, 2301
BshFI	GGCC	7 cut(s)	5, 147, 248, 402, 614, 954, 2240
BsiEI	CGRYCG	2 cut(s)	202, 2301
BsiHKAI	GWGCWC	1 cut(s)	579
BsiSI	CCGG	4 cut(s)	233, 381, 404, 1400
BslFI	GGGAC	4 cut(s)	282, 1937, 2019, 2167
BslI	CCNNNNNNNGG	7 cut(s)	310, 340, 477, 508, 553, 897, 1114
BsmAI	GTCTC	3 cut(s)	146, 729, 1032
BsmFI	GGGAC	4 cut(s)	282, 1937, 2019, 2167
BsmI	GAATGC	2 cut(s)	722, 1252
BsnI	GGCC	7 cut(s)	5, 147, 248, 402, 614, 954, 2240
Bso31I	GGTCTC	2 cut(s)	146, 1032
Bsp120I	GGGCCC	1 cut(s)	400
Bsp1286I	GDGCHC	3 cut(s)	404, 559, 579
Bsp13I	TCCGGA	1 cut(s)	1399
Bsp143I	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
Bsp19I	CCATGG	1 cut(s)	1804
Bsp68I	TCGCGA	1 cut(s)	204
BspACI	CCGC	7 cut(s)	212, 236, 281, 305, 572, 976, 1630
BspANI	GGCC	7 cut(s)	5, 147, 248, 402, 614, 954, 2240
BspCNI	CTCAG	3 cut(s)	1299, 1936, 2023
BspEI	TCCGGA	1 cut(s)	1399
BspFNI	CGCG	4 cut(s)	204, 255, 1788, 1969
BspHI	TCATGA	2 cut(s)	1159, 1894
BspLI	GGNNCC	4 cut(s)	379, 402, 613, 927
BspPI	GGATC	3 cut(s)	1148, 1861, 1925
BspTI	CTTAAG	1 cut(s)	1826
BspTNI	GGTCTC	2 cut(s)	146, 1032
BsrBI	CCGCTC	1 cut(s)	1630
BsrDI	GCAATG	4 cut(s)	465, 613, 784, 2109
BsrI	ACTGG	8 cut(s)	9, 117, 910, 998, 1137, 1691, 1721, 1833
BssECI	CCNNGG	5 cut(s)	321, 333, 955, 1486, 1804
BssMI	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
BssNI	GRCGYC	1 cut(s)	698
BssT1I	CCWWGG	4 cut(s)	321, 955, 1486, 1804
Bst2UI	CCWGG	2 cut(s)	335, 395
Bst4CI	ACNGT	7 cut(s)	260, 458, 686, 1218, 1708, 1977, 1987
Bst6I	CTCTTC	1 cut(s)	2181
BstACI	GRCGYC	1 cut(s)	698
BstAFI	CTTAAG	1 cut(s)	1826
BstBAI	YACGTR	1 cut(s)	1091
BstC8I	GCNNGC	4 cut(s)	368, 422, 493, 2169
BstDEI	CTNAG	4 cut(s)	1006, 1286, 1923, 2031
BstDSI	CCRYGG	1 cut(s)	1804
BstENI	CCTNNNNNAGG	2 cut(s)	895, 1112
BstFNI	CGCG	4 cut(s)	204, 255, 1788, 1969
BstH2I	RGCGCY	1 cut(s)	117
BstHHI	GCGC	2 cut(s)	116, 2127
BstKTI	GATC	7 cut(s)	202, 1051, 1156, 1474, 1856, 1933, 2301
BstMAI	GTCTC	3 cut(s)	146, 729, 1032
BstMBI	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
BstMCI	CGRYCG	2 cut(s)	202, 2301
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 93, 201, 236, 245, 563, 2122
BstNI	CCWGG	2 cut(s)	335, 395
BstNSI	RCATGY	1 cut(s)	724
BstPAI	GACNNNNGTC	1 cut(s)	1828
BstSCI	CCNGG	3 cut(s)	333, 393, 402
BstSFI	CTRYAG	2 cut(s)	2016, 2345
BstSLI	GKGCMC	1 cut(s)	404
BstUI	CGCG	4 cut(s)	204, 255, 1788, 1969
BstV1I	GCAGC	1 cut(s)	96
BstV2I	GAAGAC	1 cut(s)	862
BstX2I	RGATCY	2 cut(s)	1048, 1930
BstXI	CCANNNNNNTGG	2 cut(s)	993, 1709
BstYI	RGATCY	2 cut(s)	1048, 1930
BsuI	GTATCC	1 cut(s)	1230
BsuRI	GGCC	7 cut(s)	5, 147, 248, 402, 614, 954, 2240
BtgI	CCRYGG	1 cut(s)	1804
BtgZI	GCGATG	1 cut(s)	2040
BtrI	CACGTC	1 cut(s)	634
BtsI	GCAGTG	1 cut(s)	1028
BtsIMutI	CAGTG	4 cut(s)	16, 463, 1028, 1149
BtuMI	TCGCGA	1 cut(s)	204
Cac8I	GCNNGC	4 cut(s)	368, 422, 493, 2169
CciI	TCATGA	2 cut(s)	1159, 1894
CfoI	GCGC	2 cut(s)	116, 2127
Cfr13I	GGNCC	5 cut(s)	378, 400, 401, 499, 612
CseI	GACGC	2 cut(s)	284, 706
CsiI	ACCWGGT	1 cut(s)	393
Csp6I	GTAC	3 cut(s)	795, 903, 1731
CspCI	CAANNNNNGTGG	2 cut(s)	1983, 2018
CviQI	GTAC	3 cut(s)	795, 903, 1731
DdeI	CTNAG	4 cut(s)	1006, 1286, 1923, 2031
DpnI	GATC	7 cut(s)	201, 1050, 1155, 1473, 1855, 1932, 2300
DpnII	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
DraI	TTTAAA	1 cut(s)	1075
Eam1104I	CTCTTC	1 cut(s)	2181
EarI	CTCTTC	1 cut(s)	2181
EciI	GGCGGA	1 cut(s)	227
Ecl136II	GAGCTC	1 cut(s)	577
Eco130I	CCWWGG	4 cut(s)	321, 955, 1486, 1804
Eco147I	AGGCCT	1 cut(s)	147
Eco24I	GRGCYC	3 cut(s)	404, 559, 579
Eco31I	GGTCTC	2 cut(s)	146, 1032
Eco47I	GGWCC	2 cut(s)	378, 499
Eco53kI	GAGCTC	1 cut(s)	577
Eco57I	CTGAAG	3 cut(s)	1040, 1288, 1923
EcoICRI	GAGCTC	1 cut(s)	577
EcoNI	CCTNNNNNAGG	2 cut(s)	895, 1112
EcoRII	CCWGG	2 cut(s)	333, 393
EcoT14I	CCWWGG	4 cut(s)	321, 955, 1486, 1804
EcoT22I	ATGCAT	2 cut(s)	722, 1273
EcoT38I	GRGCYC	3 cut(s)	404, 559, 579
ErhI	CCWWGG	4 cut(s)	321, 955, 1486, 1804
FalI	AAGNNNNNCTT	6 cut(s)	1035, 1067, 1038, 1070, 1741, 1773
FaqI	GGGAC	4 cut(s)	282, 1937, 2019, 2167
FauI	CCCGC	2 cut(s)	312, 969
Fnu4HI	GCNGC	3 cut(s)	85, 237, 281
FriOI	GRGCYC	3 cut(s)	404, 559, 579
Fsp4HI	GCNGC	3 cut(s)	85, 237, 281
FspBI	CTAG	8 cut(s)	284, 581, 792, 1230, 1652, 1812, 2216, 2330
GlaI	GCGC	2 cut(s)	115, 2126
GluI	GCNGC	3 cut(s)	85, 237, 281
GsuI	CTGGAG	2 cut(s)	969, 1704
HaeII	RGCGCY	1 cut(s)	117
HaeIII	GGCC	7 cut(s)	5, 147, 248, 402, 614, 954, 2240
HapII	CCGG	4 cut(s)	233, 381, 404, 1400
HgaI	GACGC	2 cut(s)	284, 706
HhaI	GCGC	2 cut(s)	116, 2127
Hin1I	GRCGYC	1 cut(s)	698
Hin6I	GCGC	2 cut(s)	114, 2125
HinP1I	GCGC	2 cut(s)	114, 2125
HincII	GTYRAC	3 cut(s)	19, 631, 1365
HindII	GTYRAC	3 cut(s)	19, 631, 1365
HinfI	GANTC	6 cut(s)	170, 353, 1179, 1543, 1950, 2057
HpaI	GTTAAC	2 cut(s)	19, 1365
HpaII	CCGG	4 cut(s)	233, 381, 404, 1400
HphI	GGTGA	4 cut(s)	272, 464, 649, 2332
Hpy166II	GTNNAC	6 cut(s)	19, 631, 1365, 1696, 1820, 1973
Hpy188I	TCNGA	6 cut(s)	40, 136, 1206, 1307, 1797, 1942
Hpy188III	TCNNGA	9 cut(s)	203, 986, 1160, 1230, 1400, 1610, 1647, 1745, 1895
Hpy8I	GTNNAC	6 cut(s)	19, 631, 1365, 1696, 1820, 1973
Hpy99I	CGWCG	2 cut(s)	200, 260
HpyCH4III	ACNGT	7 cut(s)	260, 458, 686, 1218, 1708, 1977, 1987
HpyCH4IV	ACGT	4 cut(s)	21, 633, 1090, 1729
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 93, 201, 236, 245, 563, 2122
HpyF3I	CTNAG	4 cut(s)	1006, 1286, 1923, 2031
HpySE526I	ACGT	4 cut(s)	21, 633, 1090, 1729
Hsp92I	GRCGYC	1 cut(s)	698
HspAI	GCGC	2 cut(s)	114, 2125
Kpn2I	TCCGGA	1 cut(s)	1399
KspAI	GTTAAC	2 cut(s)	19, 1365
Kzo9I	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
LmnI	GCTCC	7 cut(s)	235, 563, 574, 582, 809, 1723, 1887
Lsp1109I	GCAGC	1 cut(s)	96
LweI	GCATC	8 cut(s)	217, 407, 963, 1280, 1453, 1910, 2017, 2275
MabI	ACCWGGT	1 cut(s)	393
MaeI	CTAG	8 cut(s)	284, 581, 792, 1230, 1652, 1812, 2216, 2330
MaeII	ACGT	4 cut(s)	21, 633, 1090, 1729
MaeIII	GTNAC	5 cut(s)	175, 452, 1375, 1441, 1835
MalI	GATC	7 cut(s)	201, 1050, 1155, 1473, 1855, 1932, 2300
MbiI	CCGCTC	1 cut(s)	1630
MboI	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
MflI	RGATCY	2 cut(s)	1048, 1930
MhlI	GDGCHC	3 cut(s)	404, 559, 579
MluI	ACGCGT	1 cut(s)	1967
MlyI	GAGTC	3 cut(s)	347, 1188, 1959
MmeI	TCCRAC	4 cut(s)	159, 291, 941, 2172
Mph1103I	ATGCAT	2 cut(s)	722, 1273
MroI	TCCGGA	1 cut(s)	1399
MroXI	GAANNNNTTC	1 cut(s)	1050
MslI	CAYNNNNRTG	3 cut(s)	534, 875, 1205
MspCI	CTTAAG	1 cut(s)	1826
MspI	CCGG	4 cut(s)	233, 381, 404, 1400
MspR9I	CCNGG	3 cut(s)	335, 395, 404
Mva1269I	GAATGC	2 cut(s)	722, 1252
MvaI	CCWGG	2 cut(s)	335, 395
MvnI	CGCG	4 cut(s)	204, 255, 1788, 1969
MwoI	GCNNNNNNNGC	7 cut(s)	11, 93, 201, 236, 245, 563, 2122
NciI	CCSGG	1 cut(s)	404
NcoI	CCATGG	1 cut(s)	1804
NdeII	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
NlaIV	GGNNCC	4 cut(s)	379, 402, 613, 927
NmeAIII	GCCGAG	2 cut(s)	396, 2266
NmuCI	GTSAC	3 cut(s)	175, 452, 1441
NruI	TCGCGA	1 cut(s)	204
NsiI	ATGCAT	2 cut(s)	722, 1273
NspI	RCATGY	1 cut(s)	724
PagI	TCATGA	2 cut(s)	1159, 1894
PceI	AGGCCT	1 cut(s)	147
PctI	GAATGC	2 cut(s)	722, 1252
PdmI	GAANNNNTTC	1 cut(s)	1050
PfeI	GAWTC	3 cut(s)	170, 1543, 2057
PkrI	GCNGC	3 cut(s)	86, 238, 282
Ple19I	CGATCG	2 cut(s)	202, 2301
PleI	GAGTC	3 cut(s)	347, 1187, 1958
PpsI	GAGTC	3 cut(s)	347, 1187, 1958
Ppu21I	YACGTR	1 cut(s)	1091
PshAI	GACNNNNGTC	1 cut(s)	1828
PsiI	TTATAA	1 cut(s)	410
Psp124BI	GAGCTC	1 cut(s)	579
Psp6I	CCWGG	2 cut(s)	333, 393
PspGI	CCWGG	2 cut(s)	333, 393
PspN4I	GGNNCC	4 cut(s)	379, 402, 613, 927
PspOMI	GGGCCC	1 cut(s)	400
PspPI	GGNCC	5 cut(s)	378, 400, 401, 499, 612
PsrI	GAACNNNNNNTAC	2 cut(s)	103, 135
PsuI	RGATCY	2 cut(s)	1048, 1930
PvuI	CGATCG	2 cut(s)	202, 2301
RruI	TCGCGA	1 cut(s)	204
RsaI	GTAC	3 cut(s)	796, 904, 1732
RsaNI	GTAC	3 cut(s)	795, 903, 1731
RseI	CAYNNNNRTG	3 cut(s)	534, 875, 1205
SacI	GAGCTC	1 cut(s)	579
SatI	GCNGC	3 cut(s)	85, 237, 281
Sau3AI	GATC	7 cut(s)	199, 1048, 1153, 1471, 1853, 1930, 2298
Sau96I	GGNCC	5 cut(s)	378, 400, 401, 499, 612
ScaI	AGTACT	1 cut(s)	796
SchI	GAGTC	3 cut(s)	347, 1188, 1959
ScrFI	CCNGG	3 cut(s)	335, 395, 404
SduI	GDGCHC	3 cut(s)	404, 559, 579
SexAI	ACCWGGT	1 cut(s)	393
SfaNI	GCATC	8 cut(s)	217, 407, 963, 1280, 1453, 1910, 2017, 2275
SfcI	CTRYAG	2 cut(s)	2016, 2345
SinI	GGWCC	2 cut(s)	378, 499
SmiMI	CAYNNNNRTG	3 cut(s)	534, 875, 1205
SmlI	CTYRAG	4 cut(s)	149, 1826, 2162, 2312
SmoI	CTYRAG	4 cut(s)	149, 1826, 2162, 2312
SpeI	ACTAGT	1 cut(s)	791
SseBI	AGGCCT	1 cut(s)	147
SsiI	CCGC	7 cut(s)	212, 236, 281, 305, 572, 976, 1630
SspMI	CTAG	8 cut(s)	284, 581, 792, 1230, 1652, 1812, 2216, 2330
SstI	GAGCTC	1 cut(s)	579
StuI	AGGCCT	1 cut(s)	147
StyD4I	CCNGG	3 cut(s)	333, 393, 402
StyI	CCWWGG	4 cut(s)	321, 955, 1486, 1804
TaaI	ACNGT	7 cut(s)	260, 458, 686, 1218, 1708, 1977, 1987
TaiI	ACGT	4 cut(s)	24, 636, 1093, 1732
TaqI	TCGA	5 cut(s)	141, 168, 356, 1146, 2048
TaqII	GACCGA	1 cut(s)	874
TatI	WGTACW	1 cut(s)	794
TauI	GCSGC	2 cut(s)	239, 283
TfiI	GAWTC	3 cut(s)	170, 1543, 2057
TscAI	CASTG	4 cut(s)	16, 463, 1028, 1156
TseFI	GTSAC	3 cut(s)	175, 452, 1441
TseI	GCWGC	1 cut(s)	84
Tsp45I	GTSAC	3 cut(s)	175, 452, 1441
TspDTI	ATGAA	5 cut(s)	503, 1137, 1245, 1959, 2079
TspGWI	ACGGA	3 cut(s)	112, 163, 2297
TspRI	CASTG	4 cut(s)	16, 463, 1028, 1156
Vha464I	CTTAAG	1 cut(s)	1826
VpaK11BI	GGWCC	2 cut(s)	378, 499
XagI	CCTNNNNNAGG	2 cut(s)	895, 1112
XapI	RAATTY	1 cut(s)	1671
XbaI	TCTAGA	1 cut(s)	1229
XceI	RCATGY	1 cut(s)	724
XmnI	GAANNNNTTC	1 cut(s)	1050
XspI	CTAG	8 cut(s)	284, 581, 792, 1230, 1652, 1812, 2216, 2330
ZrmI	AGTACT	1 cut(s)	796
Zsp2I	ATGCAT	2 cut(s)	722, 1273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.