MD13G1132900.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016682
Physical Location & Seq
Forward (+)
10119063 .. 10119902
840 bp
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UTR
Exon/CDS
Intron
MD13G1132900.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGACTTCTTCACAGCCTCACCTTCTAAATCAAACAACTCACCATCATCTTCATCAAAAACCAAAAGAAAGCAACAACAGCAACAAACCCAGCAACCACAAACCCAGCAGGAGACTAGGTTTCTTGGAGTGAGGAGAAGGCCGTGGGGCAGATATGCGGCTGAGATACGAGACCCTTCGACCAAAGAGAGGCATTGGCTCGGAACCTTCGATACGGCGGAGGAGGCCGCCTTGGCCTACGACCGAGCCGCTCGCTCCATGCGCGGTTCCAAGGCTCGAACCAACTTTGTCTATTCAGACATGCCACATGGCTCGTCTGTCACCTCAATTATCTCACCCGACGAATCGCAACGCGACATGTCAGCGTTCTTCACTACCCCACTTCCCCAAACCCACTCCCAGTCTCAAACACACACCCAGCAGCCCATATTCACTCATCAGGACCCATTTGATGCCTACACAGTCTCTGGGGGTTCATCAACAGAGTTCGGAAGCGGGTCTTATGGGCCCATTTCCGGCGTTGTTGCTGATGAATCCGGGTATCAGTCTCAATCTCACACTCATGAGCTACCTCCTTTGCCTCCTTGTGTCTCCTCCTCCACTTGTTATGAGCCTGAAGGCATTATGAGCTCGGACGGAGTTTGGAATGAGTCGGGCTTTTACGGGTTTTCACAGCCCACGACGAACAATGGGCTGGACTCATTGGTGAGTGGGTCCTATGTGGGCTTTGACTCGAACGAGTTTGTGCAGCACAGCCCATTGTTTGGGAGCATGCCACCTGTCTCGGATGCGGGTATTGATGGGTTTGATTTGGGTTCATCCTCTGCCTATTTCTACTGA

Protein Analysis

280

Amino Acids

30.53

Weight (kDa)

5.19

Isoelectric Point (pI)

68.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 40 - 90 4.4e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016682)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13910
fragaria_vesca FvH4_4g22310
malus_domestica MD13G1132900.v1.1 MD16G1143300.v1.1
prunus_persica Prupe.1G212700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0428551
rosa_laevigata RLG00000007143
rosa_roxburghii Rroxscaffold_5G00370260
rosa_rugosa Rorug04G0226200
rosa_samantha Rh4AG283800 Rh4BG289500 Rh4CG305400 Rh4DG286500
rosa_wichuraiana Rw4G024630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 764
AccBSI CCGCTC 1 cut(s) 251
AccII CGCG 2 cut(s) 264, 354
AciI CCGC 7 cut(s) 158, 218, 228, 249, 264, 495, 791
AcuI CTGAAG 1 cut(s) 636
AfiI CCNNNNNNNGG 3 cut(s) 189, 515, 764
AflIII ACRYGT 1 cut(s) 357
AluBI AGCT 2 cut(s) 568, 630
AluI AGCT 2 cut(s) 568, 630
Alw21I GWGCWC 1 cut(s) 632
Alw26I GTCTC 7 cut(s) 107, 165, 408, 469, 552, 595, 787
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 4 cut(s) 140, 225, 234, 506
ApaI GGGCCC 1 cut(s) 510
ApeKI GCWGC 2 cut(s) 421, 748
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 4 cut(s) 442, 506, 507, 714
AsuC2I CCSGG 1 cut(s) 538
AsuHPI GGTGA 5 cut(s) 12, 33, 313, 327, 718
AvaII GGWCC 2 cut(s) 442, 714
BaeGI GKGCMC 1 cut(s) 510
BanII GRGCYC 2 cut(s) 510, 632
Bbv12I GWGCWC 1 cut(s) 632
BbvI GCAGC 2 cut(s) 433, 760
BccI CCATC 2 cut(s) 52, 794
BceAI ACGGC 2 cut(s) 127, 231
BcnI CCSGG 1 cut(s) 538
BcoDI GTCTC 7 cut(s) 107, 165, 408, 469, 552, 595, 787
BfaI CTAG 1 cut(s) 117
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 5 cut(s) 159, 228, 249, 422, 749
BlsI GCNGC 5 cut(s) 160, 229, 250, 423, 750
Bme1390I CCNGG 1 cut(s) 538
Bme18I GGWCC 2 cut(s) 442, 714
BmgT120I GGNCC 4 cut(s) 442, 506, 507, 714
BmiI GGNNCC 5 cut(s) 205, 268, 444, 508, 715
BmrFI CCNGG 1 cut(s) 538
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 2 cut(s) 442, 778
BmuI ACTGGG 1 cut(s) 394
BpuMI CCSGG 1 cut(s) 538
BsaI GGTCTC 1 cut(s) 165
BsaJI CCNNGG 3 cut(s) 143, 231, 270
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 515, 764
Bse1I ACTGG 1 cut(s) 400
BseDI CCNNGG 3 cut(s) 143, 231, 270
BseGI GGATG 2 cut(s) 793, 818
BseLI CCNNNNNNNGG 3 cut(s) 189, 515, 764
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 4 cut(s) 148, 236, 583, 586
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 2 cut(s) 433, 760
BseYI CCCAGC 3 cut(s) 90, 105, 417
BsgI GTGCAG 1 cut(s) 767
Bsh1236I CGCG 2 cut(s) 264, 354
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 4 cut(s) 142, 227, 236, 508
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 632
BsiSI CCGG 2 cut(s) 516, 537
BslI CCNNNNNNNGG 3 cut(s) 189, 515, 764
BsmAI GTCTC 7 cut(s) 107, 165, 408, 469, 552, 595, 787
BsnI GGCC 4 cut(s) 142, 227, 236, 508
Bso31I GGTCTC 1 cut(s) 165
Bsp120I GGGCCC 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 510, 632
BspACI CCGC 7 cut(s) 158, 218, 228, 249, 264, 495, 791
BspANI GGCC 4 cut(s) 142, 227, 236, 508
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 2 cut(s) 264, 354
BspHI TCATGA 1 cut(s) 562
BspLI GGNNCC 5 cut(s) 205, 268, 444, 508, 715
BspTNI GGTCTC 1 cut(s) 165
BsrBI CCGCTC 1 cut(s) 251
BsrI ACTGG 1 cut(s) 400
BssECI CCNNGG 3 cut(s) 143, 231, 270
BssT1I CCWWGG 2 cut(s) 231, 270
Bst4CI ACNGT 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 253, 773
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 143
BstF5I GGATG 2 cut(s) 793, 818
BstFNI CGCG 2 cut(s) 264, 354
BstHHI GCGC 1 cut(s) 264
BstMAI GTCTC 7 cut(s) 107, 165, 408, 469, 552, 595, 787
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 5 cut(s) 79, 224, 233, 261, 627
BstNSI RCATGY 3 cut(s) 304, 361, 775
BstSCI CCNGG 1 cut(s) 536
BstSLI GKGCMC 1 cut(s) 510
BstUI CGCG 2 cut(s) 264, 354
BstV1I GCAGC 2 cut(s) 433, 760
BsuRI GGCC 4 cut(s) 142, 227, 236, 508
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 2 cut(s) 793, 818
Cac8I GCNNGC 2 cut(s) 253, 773
CaiI CAGNNNCTG 1 cut(s) 467
CciI TCATGA 1 cut(s) 562
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 4 cut(s) 442, 506, 507, 714
CviAII CATG 6 cut(s) 259, 301, 308, 358, 563, 772
DdeI CTNAG 1 cut(s) 162
EciI GGCGGA 1 cut(s) 233
Ecl136II GAGCTC 1 cut(s) 630
Eco130I CCWWGG 2 cut(s) 231, 270
Eco24I GRGCYC 2 cut(s) 510, 632
Eco31I GGTCTC 1 cut(s) 165
Eco47I GGWCC 2 cut(s) 442, 714
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 1 cut(s) 636
EcoICRI GAGCTC 1 cut(s) 630
EcoO109I RGGNCCY 2 cut(s) 442, 714
EcoT14I CCWWGG 2 cut(s) 231, 270
EcoT38I GRGCYC 2 cut(s) 510, 632
ErhI CCWWGG 2 cut(s) 231, 270
FaeI CATG 6 cut(s) 262, 304, 311, 361, 566, 775
FalI AAGNNNNNCTT 2 cut(s) 484, 516
FatI CATG 6 cut(s) 258, 300, 307, 357, 562, 771
FauI CCCGC 2 cut(s) 488, 784
Fnu4HI GCNGC 5 cut(s) 159, 228, 249, 422, 749
FokI GGATG 2 cut(s) 800, 805
FriOI GRGCYC 2 cut(s) 510, 632
Fsp4HI GCNGC 5 cut(s) 159, 228, 249, 422, 749
FspBI CTAG 1 cut(s) 117
GlaI GCGC 1 cut(s) 263
GluI GCNGC 5 cut(s) 159, 228, 249, 422, 749
GsaI CCCAGC 3 cut(s) 94, 109, 421
HaeIII GGCC 4 cut(s) 142, 227, 236, 508
HapII CCGG 2 cut(s) 516, 537
HhaI GCGC 1 cut(s) 264
Hin1II CATG 6 cut(s) 262, 304, 311, 361, 566, 775
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HinfI GANTC 5 cut(s) 344, 533, 650, 698, 731
HpaII CCGG 2 cut(s) 516, 537
HphI GGTGA 5 cut(s) 12, 33, 313, 327, 718
Hpy188I TCNGA 5 cut(s) 203, 298, 491, 634, 787
Hpy188III TCNNGA 2 cut(s) 440, 563
Hpy99I CGWCG 2 cut(s) 344, 685
HpyAV CCTTC 5 cut(s) 33, 132, 186, 217, 611
HpyCH4III ACNGT 1 cut(s) 463
HpyCH4V TGCA 1 cut(s) 748
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 224, 233, 261, 627
HpyF3I CTNAG 1 cut(s) 162
Hsp92II CATG 6 cut(s) 262, 304, 311, 361, 566, 775
HspAI GCGC 1 cut(s) 262
LmnI GCTCC 2 cut(s) 260, 768
Lsp1109I GCAGC 2 cut(s) 433, 760
LweI GCATC 2 cut(s) 442, 778
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 319
MbiI CCGCTC 1 cut(s) 251
MboII GAAGA 2 cut(s) 42, 361
MhlI GDGCHC 2 cut(s) 510, 632
MluCI AATT 1 cut(s) 327
MlyI GAGTC 3 cut(s) 659, 692, 725
MslI CAYNNNNRTG 1 cut(s) 561
MspI CCGG 2 cut(s) 516, 537
MspR9I CCNGG 1 cut(s) 538
MvnI CGCG 2 cut(s) 264, 354
MwoI GCNNNNNNNGC 5 cut(s) 79, 224, 233, 261, 627
NciI CCSGG 1 cut(s) 538
NlaIII CATG 6 cut(s) 262, 304, 311, 361, 566, 775
NlaIV GGNNCC 5 cut(s) 205, 268, 444, 508, 715
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 3 cut(s) 304, 361, 775
PaeI GCATGC 1 cut(s) 775
PagI TCATGA 1 cut(s) 562
PciI ACATGT 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PfeI GAWTC 2 cut(s) 344, 533
PflMI CCANNNNNTGG 1 cut(s) 764
PkrI GCNGC 5 cut(s) 160, 229, 250, 423, 750
PleI GAGTC 3 cut(s) 658, 692, 725
PpsI GAGTC 3 cut(s) 658, 692, 725
PpuMI RGGWCCY 2 cut(s) 442, 714
PscI ACATGT 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 632
Psp5II RGGWCCY 2 cut(s) 442, 714
PspFI CCCAGC 3 cut(s) 90, 105, 417
PspN4I GGNNCC 5 cut(s) 205, 268, 444, 508, 715
PspOMI GGGCCC 1 cut(s) 506
PspPI GGNCC 4 cut(s) 442, 506, 507, 714
PspPPI RGGWCCY 2 cut(s) 442, 714
PsrI GAACNNNNNNTAC 2 cut(s) 196, 228
PstNI CAGNNNCTG 1 cut(s) 467
RseI CAYNNNNRTG 1 cut(s) 561
SacI GAGCTC 1 cut(s) 632
SatI GCNGC 5 cut(s) 159, 228, 249, 422, 749
Sau96I GGNCC 4 cut(s) 442, 506, 507, 714
SchI GAGTC 3 cut(s) 659, 692, 725
ScrFI CCNGG 1 cut(s) 538
SduI GDGCHC 2 cut(s) 510, 632
SetI ASST 8 cut(s) 25, 122, 209, 326, 570, 574, 632, 781
SfaNI GCATC 2 cut(s) 442, 778
SfiI GGCCNNNNNGGCC 1 cut(s) 233
SinI GGWCC 2 cut(s) 442, 714
SmiMI CAYNNNNRTG 1 cut(s) 561
SphI GCATGC 1 cut(s) 775
Sse9I AATT 1 cut(s) 327
SsiI CCGC 7 cut(s) 158, 218, 228, 249, 264, 495, 791
SspMI CTAG 1 cut(s) 117
SstI GAGCTC 1 cut(s) 632
StyD4I CCNGG 1 cut(s) 536
StyI CCWWGG 2 cut(s) 231, 270
TaaI ACNGT 1 cut(s) 463
TaqI TCGA 4 cut(s) 179, 210, 277, 734
TaqII GACCGA 1 cut(s) 258
TasI AATT 1 cut(s) 327
TauI GCSGC 3 cut(s) 161, 230, 251
TfiI GAWTC 2 cut(s) 344, 533
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 2 cut(s) 421, 748
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 4 cut(s) 42, 465, 546, 807
TspGWI ACGGA 1 cut(s) 651
Van91I CCANNNNNTGG 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 442, 714
XceI RCATGY 3 cut(s) 304, 361, 775
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.