MD13G1136600.v1.1

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009386
Physical Location & Seq
Forward (+)
10489466 .. 10496949
7484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1136600.v1.1.491

Sequence Viewer

Length: 2886 bp
ATGGGAAAACGAATATGGATTCCCTGGGCGATCATGGTGCTCCACGTCGTCGCCATTCTCTTATTCACCAGAGGCTTTCTTCTCACCCGGACGGAGCTTCCATTTTACAGCAACTGCTCCGATGTTTCGCAGTCGCCTTGCTCCTACTCCGTTCCAGAAACCCAGAACCGAAACGACACCGTTGACCCGAATCAGCAGCGTTGCTGGAGCAAGCCTGCTGTTGGGCGCCTTGTAATCATCGTTTTCGACGCTCTCAGGTTTGATTTTGTGGCTCCCAGTACTTTCTTTCAAGAATCAAAACCATGGATGGACAAATTAAAATTTGTTCAAGACATGGCAGCCAGAAATGCGTCGTCTGCTCGGATTTTTAAAGCCATTGCTGATCCGCCAACGACGAGTTTGCAGCGTTTGAAGGGCCTGACAACCGGTGGATTGCCGACTTTTATTGATGTTGGGAACAGCTTTGGTGCTCCAGCAATAGTGGAAGATAACTTGATACATCAGTTGGCTAAAAATGGAAAGCGAGTTGTAATGATGGGGGATGATACATGGACACAGTTGTTTCCTCGTCATTTTGAAAAATCTTTTCCTTACCCCTCTTTCAATGTTAGAGATCTTGATACGGTGGACAATGGCTGCATTGAGCACCTTCTTCCATTCTTGTACCAAGAAGATTGGGATGTTCTTATTGCACACTTTCTTGGTGTGGATCATGCTGGGCACATATTTGGGGTTGATTCTATGCAAATGATAGAAAAGTTGGAGCAATACAACAACGTCTTACTGAAAGTAGTTGAAGCACTGGAGAGGCAGTCTGCGCCAGGGGGCTTACATGAGAATACTCTTTTGCTTGTGATGGGCGATCATGGTCAGACTGTGAATGGAGATCATGGTGGGGGGAGTTCTGAAGAGGTTGAGACATCATTATTTGCTGTGAGCTTTAAGAATCCTCCATCTCCCATACCATCAGAGTTTGATACTTCATCTTGTGAACTTGATTTGGATGGGAGGAATATTTGTGCAAGTACCATCCAGCAGCTTGACTTTGCGGTGACAATATCAGCGCTGCTTGGTATCCCTTTTCCTTTTGGAAGTATTGGGCGTGTTAATCCTCAGCTTTATGCTTTAGGAGCTGGAACATGGAATTTTGAAGATAGTGTGGGCAATTCCCAAAATCAGTCAAAGTTGGAACAATGGATGTTGAATTATGCCAATGTACTCTGCACCAATTCTTGGCAGGTAAAAAGATATATAGATATATATTCTGCTTTATCAGTTATTGGATTTTCCCATGAAGATTTGTTGCACATAGCAGACATGTATGCTAAAGCAGAGGAGAGATGGTCACATACTACAAAGAAGTTGCTGTCACATGAAAAGAAAAGCCACAATGAATTATTGCCTGCTCTTAAGAGGCAAATTAATTTGTTTTCTGATTTCCTAGCAAGTGTTGCCGAACTTGCACGTTCTAAATGGACTGAGTTTAATTTAAAGATGATGGGTGCTGGACTTGGCATTATGCTGATATCACTTCTTGTCCATTTCCTTGCAATTAAGAAAGTGAAGGAGGAATATGGTTTTTCTTTTACTTCATCTGGAGATTCTGGGATTTCCTTCAGTTTAATTTTTTCTTGTTTTATGGTGGTGATGCGTGCATGCAGCTTCCTCTCGAATAGTTTTATCTTGGAAGAAGGAAAAGTGGCATGCTTTCTTTTGGCCACAACTGGACTAATTAAGATGCGATATTCAATCATGAAGAAGAAGATGATATTAGAAGCATTTGTTTTCCTTCTTCTGATCACCATTTGCAGATTTACTATTGAAGTTGGATTGTCAAAGCAGGGTCCTAGTTCTGAAATTATTAATGCATATCCTTCATGGATGCTCAGGATCACTGCAGGGTTTCCAGTTTGGAATATTGTTGCTGAAGTTATACCGGCAGTTTCCCTGATTTTATTGGCATTTTTGCTGCGCAAGGCCATTACTGGAAGCTCTTCTGAGGGGATTTGGAAGTATATTATCATGGGAACTAATATAAGTTATATACTAATAGCAGTGCATTGGGCTTCAGAAAGTAACATATTCAATCTGGCCGAGGTGCTTAAGGGCATTGGAAGAAATTACATTCCTCGTCTGATATATGCCATTGGATTTGGACAATTGTTATTACTGGCATTTAATCAGCTTTTCAATAAGGGGAAATCCTCGGATTGCAGCAAAGTCTTATATATTAAAACAGTGGCCATGTTTTCTGCCTGGAGCTCAACAGTCATTCTTCTGTTGGGAAAGCAGGGGCCTTGGATTGCTTTAGCATTCATTATTGGAGGTTATTGCATAATGAGGCTGGATAACATAGAGCTGGATGCCAAAGATGGAGGCAGTTGGAATACAATGCTTGATCCTATTCCTGTAACGCAGTGGAGCCTTTTTGCTGTGTGCCTGTTTTTCTGCACCGGTCATTGGTGTGCTTTTGACGGCCTCCGATATGGTGCTGCATTTATCGGGTTCGATGATTTCATCCTAGTTCCCCAAGCAATCCTCCTTACAATGGATACATTTGGTTTTTCTCTCATCCTTCCAATATTTGGACTTCCATTTCTTGTTGCACGCCTGGGTCAGACCGAGAAAGGGAAGAAATTTGTGCTCACACGACTATCTCTAGTGTACATGATTTATGGGCTCATCATGGCTACTTCGGTCACAGCAACTATAATATGCGTTACAATGCACAGGCGGCATCTAATGGTATGGGGTTTATTTGCCCCGAAGTTTGTTTTCGATGTGGCGGGTCTTATCCTTACAGACACACTCGTTTGTTTGGCCTCACATTACTACTTCAGTCAAGTAGAAGACGACTCCCTACAAGATCCGTTCTCCAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest

Protein Analysis

962

Amino Acids

107.36

Weight (kDa)

6.51

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphodiest PF01663 209 - 292 1.9e-09 Type I phosphodiesterase / nucleotide pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1973
Acc36I ACCTGC 1 cut(s) 1228
AccB1I GGYRCC 1 cut(s) 225
AccB7I CCANNNNNTGG 2 cut(s) 1233, 2585
AciI CCGC 4 cut(s) 386, 1049, 2734, 2786
AclWI GGATC 5 cut(s) 377, 717, 1898, 2393, 2861
AcoI YGGCCR 3 cut(s) 1716, 2091, 2241
AcsI RAATTY 3 cut(s) 320, 1144, 2636
AcuI CTGAAG 5 cut(s) 927, 1600, 1947, 2052, 2821
AcyI GRCGYC 1 cut(s) 226
AfaI GTAC 5 cut(s) 280, 665, 1027, 1218, 2666
AfeI AGCGCT 1 cut(s) 1065
AfiI CCNNNNNNNGG 6 cut(s) 221, 1233, 2460, 2548, 2585, 2628
AflII CTTAAG 2 cut(s) 1409, 2102
AflIII ACRYGT 1 cut(s) 1317
AgeI ACCGGT 2 cut(s) 425, 2453
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 4 cut(s) 23, 820, 2255, 2610
Alw21I GWGCWC 5 cut(s) 42, 472, 648, 2264, 2646
Alw26I GTCTC 1 cut(s) 911
AlwI GGATC 5 cut(s) 377, 717, 1898, 2393, 2861
AlwNI CAGNNNCTG 1 cut(s) 114
Aor51HI AGCGCT 1 cut(s) 1065
AoxI GGCC 8 cut(s) 415, 1716, 1977, 2091, 2241, 2294, 2476, 2820
ApoI RAATTY 3 cut(s) 320, 1144, 2636
AseI ATTAAT 2 cut(s) 1422, 1863
AsiGI ACCGGT 2 cut(s) 425, 2453
Asp700I GAANNNNTTC 1 cut(s) 1993
AspLEI GCGC 4 cut(s) 228, 820, 1066, 1974
AspS9I GGNCC 3 cut(s) 415, 1844, 2294
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 5 cut(s) 58, 76, 1063, 1657, 1792
AvaII GGWCC 1 cut(s) 1844
BaeGI GKGCMC 1 cut(s) 723
BaeI ACNNNNGTAYC 2 cut(s) 488, 521
BalI TGGCCA 2 cut(s) 1718, 2243
BanI GGYRCC 1 cut(s) 225
BanII GRGCYC 2 cut(s) 2264, 2682
BbsI GAAGAC 1 cut(s) 2856
Bbv12I GWGCWC 5 cut(s) 42, 472, 648, 2264, 2646
BbvCI CCTCAGC 1 cut(s) 1113
BceAI ACGGC 1 cut(s) 2491
BcgI CGANNNNNNTGC 4 cut(s) 19, 53, 382, 416
BciT130I CCWGG 4 cut(s) 25, 822, 2257, 2612
BciVI GTATCC 2 cut(s) 1085, 2545
BclI TGATCA 1 cut(s) 1797
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 911
BfaI CTAG 4 cut(s) 1442, 1848, 2522, 2660
BfmI CTRYAG 1 cut(s) 1896
BfoI RGCGCY 2 cut(s) 229, 1067
BfrI CTTAAG 2 cut(s) 1409, 2102
BfuAI ACCTGC 1 cut(s) 1228
BfuI GTATCC 2 cut(s) 1085, 2545
BglII AGATCT 1 cut(s) 613
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 5 cut(s) 25, 88, 822, 2257, 2612
Bme18I GGWCC 1 cut(s) 1844
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 415, 1844, 2294
BmiI GGNNCC 5 cut(s) 227, 273, 1845, 2295, 2423
BmrFI CCNGG 5 cut(s) 25, 88, 822, 2257, 2612
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 5 cut(s) 1638, 1728, 1872, 2353, 2746
BmuI ACTGGG 1 cut(s) 270
BpiI GAAGAC 1 cut(s) 2856
BpmI CTGGAG 5 cut(s) 226, 456, 824, 1617, 2278
Bpu10I CCTNAGC 2 cut(s) 1113, 1886
BpuMI CCSGG 1 cut(s) 88
BsaHI GRCGYC 1 cut(s) 226
BsaJI CCNNGG 8 cut(s) 23, 24, 302, 821, 2094, 2205, 2297, 2611
BsaWI WCCGGW 2 cut(s) 425, 2453
BsaXI ACNNNNNCTCC 4 cut(s) 797, 827, 1328, 1358
Bsc4I CCNNNNNNNGG 6 cut(s) 221, 1233, 2460, 2548, 2585, 2628
Bse118I RCCGGY 3 cut(s) 425, 1936, 2453
Bse1I ACTGG 6 cut(s) 276, 807, 1729, 1907, 1990, 2175
Bse3DI GCAATG 1 cut(s) 375
BseBI CCWGG 4 cut(s) 25, 822, 2257, 2612
BseDI CCNNGG 8 cut(s) 23, 24, 302, 821, 2094, 2205, 2297, 2611
BseLI CCNNNNNNNGG 6 cut(s) 221, 1233, 2460, 2548, 2585, 2628
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 5 cut(s) 268, 1127, 1470, 1900, 1989
BseNI ACTGG 6 cut(s) 276, 807, 1729, 1907, 1990, 2175
BseRI GAGGAG 1 cut(s) 1349
BseSI GKGCMC 1 cut(s) 723
BseYI CCCAGC 1 cut(s) 716
BsgI GTGCAG 2 cut(s) 1207, 2434
BshFI GGCC 8 cut(s) 417, 1718, 1979, 2093, 2243, 2296, 2478, 2822
BshNI GGYRCC 1 cut(s) 225
BshTI ACCGGT 2 cut(s) 425, 2453
BsiHKAI GWGCWC 5 cut(s) 42, 472, 648, 2264, 2646
BsiSI CCGG 4 cut(s) 88, 426, 1937, 2454
BslI CCNNNNNNNGG 6 cut(s) 221, 1233, 2460, 2548, 2585, 2628
BsmAI GTCTC 1 cut(s) 911
BsmI GAATGC 1 cut(s) 2312
BsnI GGCC 8 cut(s) 417, 1718, 1979, 2093, 2243, 2296, 2478, 2822
Bsp1286I GDGCHC 7 cut(s) 42, 472, 648, 723, 2264, 2646, 2682
Bsp1407I TGTACA 1 cut(s) 2664
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 4 cut(s) 386, 1049, 2734, 2786
BspANI GGCC 8 cut(s) 417, 1718, 1979, 2093, 2243, 2296, 2478, 2822
BspCNI CTCAG 5 cut(s) 267, 1126, 1471, 1899, 1990
BspHI TCATGA 1 cut(s) 1752
BspLI GGNNCC 5 cut(s) 227, 273, 1845, 2295, 2423
BspMAI CTGCAG 1 cut(s) 1900
BspMI ACCTGC 1 cut(s) 1228
BspPI GGATC 5 cut(s) 377, 717, 1898, 2393, 2861
BspQI GCTCTTC 1 cut(s) 1999
BspT107I GGYRCC 1 cut(s) 225
BspTI CTTAAG 2 cut(s) 1409, 2102
BsrDI GCAATG 1 cut(s) 375
BsrFI RCCGGY 3 cut(s) 425, 1936, 2453
BsrGI TGTACA 1 cut(s) 2664
BsrI ACTGG 6 cut(s) 276, 807, 1729, 1907, 1990, 2175
BssAI RCCGGY 3 cut(s) 425, 1936, 2453
BssECI CCNNGG 8 cut(s) 23, 24, 302, 821, 2094, 2205, 2297, 2611
BssNI GRCGYC 1 cut(s) 226
BssT1I CCWWGG 2 cut(s) 302, 2297
Bst2UI CCWGG 4 cut(s) 25, 822, 2257, 2612
Bst4CI ACNGT 6 cut(s) 181, 558, 625, 877, 2239, 2269
Bst6I CTCTTC 2 cut(s) 903, 1999
BstACI GRCGYC 1 cut(s) 226
BstAFI CTTAAG 2 cut(s) 1409, 2102
BstAPI GCANNNNNTGC 1 cut(s) 1451
BstAUI TGTACA 1 cut(s) 2664
BstC8I GCNNGC 7 cut(s) 212, 216, 1404, 1653, 1657, 1705, 2608
BstDEI CTNAG 5 cut(s) 254, 1113, 1479, 1886, 1998
BstDSI CCRYGG 1 cut(s) 302
BstH2I RGCGCY 2 cut(s) 229, 1067
BstHHI GCGC 4 cut(s) 228, 820, 1066, 1974
BstMAI GTCTC 1 cut(s) 911
BstMWI GCNNNNNNNGC 7 cut(s) 347, 356, 817, 1130, 1451, 1460, 2734
BstNI CCWGG 4 cut(s) 25, 822, 2257, 2612
BstNSI RCATGY 3 cut(s) 1321, 1659, 1707
BstSCI CCNGG 5 cut(s) 23, 86, 820, 2255, 2610
BstSFI CTRYAG 1 cut(s) 1896
BstSLI GKGCMC 1 cut(s) 723
BstV2I GAAGAC 1 cut(s) 2856
BstX2I RGATCY 2 cut(s) 613, 2866
BstYI RGATCY 2 cut(s) 613, 2866
BsuI GTATCC 2 cut(s) 1085, 2545
BsuRI GGCC 8 cut(s) 417, 1718, 1979, 2093, 2243, 2296, 2478, 2822
BtgI CCRYGG 1 cut(s) 302
BtrI CACGTC 1 cut(s) 46
BtsI GCAGTG 3 cut(s) 1893, 2061, 2423
BtsIMutI CAGTG 5 cut(s) 800, 1893, 2061, 2244, 2423
BveI ACCTGC 1 cut(s) 1228
Cac8I GCNNGC 7 cut(s) 212, 216, 1404, 1653, 1657, 1705, 2608
CaiI CAGNNNCTG 1 cut(s) 114
CciI TCATGA 1 cut(s) 1752
CfoI GCGC 4 cut(s) 228, 820, 1066, 1974
Cfr10I RCCGGY 3 cut(s) 425, 1936, 2453
Cfr13I GGNCC 3 cut(s) 415, 1844, 2294
CseI GACGC 2 cut(s) 257, 339
Csp6I GTAC 5 cut(s) 279, 664, 1026, 1217, 2665
CspAI ACCGGT 2 cut(s) 425, 2453
CviQI GTAC 5 cut(s) 279, 664, 1026, 1217, 2665
DdeI CTNAG 5 cut(s) 254, 1113, 1479, 1886, 1998
DinI GGCGCC 1 cut(s) 227
DraI TTTAAA 2 cut(s) 370, 1491
EaeI YGGCCR 3 cut(s) 1716, 2091, 2241
Eam1104I CTCTTC 2 cut(s) 903, 1999
EarI CTCTTC 2 cut(s) 903, 1999
EciI GGCGGA 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 2262
Eco130I CCWWGG 2 cut(s) 302, 2297
Eco24I GRGCYC 2 cut(s) 2264, 2682
Eco32I GATATC 1 cut(s) 1527
Eco47I GGWCC 1 cut(s) 1844
Eco47III AGCGCT 1 cut(s) 1065
Eco53kI GAGCTC 1 cut(s) 2262
Eco57I CTGAAG 5 cut(s) 927, 1600, 1947, 2052, 2821
EcoICRI GAGCTC 1 cut(s) 2262
EcoO109I RGGNCCY 3 cut(s) 415, 1844, 2294
EcoRII CCWGG 4 cut(s) 23, 820, 2255, 2610
EcoRV GATATC 1 cut(s) 1527
EcoT14I CCWWGG 2 cut(s) 302, 2297
EcoT22I ATGCAT 1 cut(s) 1870
EcoT38I GRGCYC 2 cut(s) 2264, 2682
EgeI GGCGCC 1 cut(s) 227
EheI GGCGCC 1 cut(s) 227
ErhI CCWWGG 2 cut(s) 302, 2297
FauI CCCGC 1 cut(s) 2779
FbaI TGATCA 1 cut(s) 1797
FriOI GRGCYC 2 cut(s) 2264, 2682
FspBI CTAG 4 cut(s) 1442, 1848, 2522, 2660
FspI TGCGCA 1 cut(s) 1973
GlaI GCGC 4 cut(s) 227, 819, 1065, 1973
GsaI CCCAGC 1 cut(s) 720
GsuI CTGGAG 5 cut(s) 226, 456, 824, 1617, 2278
HaeII RGCGCY 2 cut(s) 229, 1067
HaeIII GGCC 8 cut(s) 417, 1718, 1979, 2093, 2243, 2296, 2478, 2822
HapII CCGG 4 cut(s) 88, 426, 1937, 2454
HgaI GACGC 2 cut(s) 257, 339
HhaI GCGC 4 cut(s) 228, 820, 1066, 1974
Hin1I GRCGYC 1 cut(s) 226
Hin6I GCGC 4 cut(s) 226, 818, 1064, 1972
HinP1I GCGC 4 cut(s) 226, 818, 1064, 1972
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 7 cut(s) 19, 190, 293, 737, 946, 1601, 2855
HpaII CCGG 4 cut(s) 88, 426, 1937, 2454
HphI GGTGA 5 cut(s) 58, 76, 1063, 1657, 1792
Hpy166II GTNNAC 4 cut(s) 184, 628, 992, 2665
Hpy188III TCNNGA 8 cut(s) 155, 290, 329, 617, 1596, 1669, 1753, 1888
Hpy8I GTNNAC 4 cut(s) 184, 628, 992, 2665
Hpy99I CGWCG 5 cut(s) 50, 53, 251, 355, 397
HpyAV CCTTC 8 cut(s) 406, 659, 1558, 1624, 1685, 1799, 1884, 2585
HpyCH4III ACNGT 6 cut(s) 181, 558, 625, 877, 2239, 2269
HpyCH4IV ACGT 3 cut(s) 45, 777, 1465
HpyF10VI GCNNNNNNNGC 7 cut(s) 347, 356, 817, 1130, 1451, 1460, 2734
HpyF3I CTNAG 5 cut(s) 254, 1113, 1479, 1886, 1998
HpySE526I ACGT 3 cut(s) 45, 777, 1465
Hsp92I GRCGYC 1 cut(s) 226
HspAI GCGC 4 cut(s) 226, 818, 1064, 1972
KasI GGCGCC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 1797
LguI GCTCTTC 1 cut(s) 1999
LweI GCATC 5 cut(s) 1638, 1728, 1872, 2353, 2746
MaeI CTAG 4 cut(s) 1442, 1848, 2522, 2660
MaeII ACGT 3 cut(s) 45, 777, 1465
MaeIII GTNAC 7 cut(s) 1051, 1344, 1368, 2075, 2410, 2698, 2719
MfeI CAATTG 1 cut(s) 2159
MflI RGATCY 2 cut(s) 613, 2866
MhlI GDGCHC 7 cut(s) 42, 472, 648, 723, 2264, 2646, 2682
MlsI TGGCCA 2 cut(s) 1718, 2243
MluNI TGGCCA 2 cut(s) 1718, 2243
Mly113I GGCGCC 1 cut(s) 226
MlyI GAGTC 1 cut(s) 2849
MmeI TCCRAC 4 cut(s) 741, 1167, 1807, 2363
Mox20I TGGCCA 2 cut(s) 1718, 2243
Mph1103I ATGCAT 1 cut(s) 1870
MroXI GAANNNNTTC 1 cut(s) 1993
MscI TGGCCA 2 cut(s) 1718, 2243
MslI CAYNNNNRTG 1 cut(s) 2463
Msp20I TGGCCA 2 cut(s) 1718, 2243
MspCI CTTAAG 2 cut(s) 1409, 2102
MspI CCGG 4 cut(s) 88, 426, 1937, 2454
MspR9I CCNGG 5 cut(s) 25, 88, 822, 2257, 2612
MunI CAATTG 1 cut(s) 2159
Mva1269I GAATGC 1 cut(s) 2312
MvaI CCWGG 4 cut(s) 25, 822, 2257, 2612
MwoI GCNNNNNNNGC 7 cut(s) 347, 356, 817, 1130, 1451, 1460, 2734
NarI GGCGCC 1 cut(s) 226
NciI CCSGG 1 cut(s) 88
NcoI CCATGG 1 cut(s) 302
NlaIV GGNNCC 5 cut(s) 227, 273, 1845, 2295, 2423
NmeAIII GCCGAG 1 cut(s) 2119
NmuCI GTSAC 4 cut(s) 1051, 1344, 1368, 2698
NsbI TGCGCA 1 cut(s) 1973
NsiI ATGCAT 1 cut(s) 1870
NspI RCATGY 3 cut(s) 1321, 1659, 1707
PaeI GCATGC 2 cut(s) 1659, 1707
PagI TCATGA 1 cut(s) 1752
PasI CCCWGGG 1 cut(s) 24
PciI ACATGT 1 cut(s) 1317
PciSI GCTCTTC 1 cut(s) 1999
PctI GAATGC 1 cut(s) 2312
PdmI GAANNNNTTC 1 cut(s) 1993
PfeI GAWTC 6 cut(s) 19, 190, 293, 737, 946, 1601
PflMI CCANNNNNTGG 2 cut(s) 1233, 2585
PinAI ACCGGT 2 cut(s) 425, 2453
PleI GAGTC 1 cut(s) 2849
PluTI GGCGCC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 2849
PpuMI RGGWCCY 1 cut(s) 1844
PscI ACATGT 1 cut(s) 1317
PshBI ATTAAT 2 cut(s) 1422, 1863
Psp124BI GAGCTC 1 cut(s) 2264
Psp5II RGGWCCY 1 cut(s) 1844
Psp6I CCWGG 4 cut(s) 23, 820, 2255, 2610
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 4 cut(s) 23, 820, 2255, 2610
PspN4I GGNNCC 5 cut(s) 227, 273, 1845, 2295, 2423
PspPI GGNCC 3 cut(s) 415, 1844, 2294
PspPPI RGGWCCY 1 cut(s) 1844
PstI CTGCAG 1 cut(s) 1900
PstNI CAGNNNCTG 1 cut(s) 114
PsuI RGATCY 2 cut(s) 613, 2866
RsaI GTAC 5 cut(s) 280, 665, 1027, 1218, 2666
RsaNI GTAC 5 cut(s) 279, 664, 1026, 1217, 2665
RseI CAYNNNNRTG 1 cut(s) 2463
SacI GAGCTC 1 cut(s) 2264
SapI GCTCTTC 1 cut(s) 1999
Sau96I GGNCC 3 cut(s) 415, 1844, 2294
ScaI AGTACT 1 cut(s) 280
SchI GAGTC 1 cut(s) 2849
ScrFI CCNGG 5 cut(s) 25, 88, 822, 2257, 2612
SduI GDGCHC 7 cut(s) 42, 472, 648, 723, 2264, 2646, 2682
SfaNI GCATC 5 cut(s) 1638, 1728, 1872, 2353, 2746
SfcI CTRYAG 1 cut(s) 1896
SfoI GGCGCC 1 cut(s) 227
SinI GGWCC 1 cut(s) 1844
SmiMI CAYNNNNRTG 1 cut(s) 2463
SmlI CTYRAG 2 cut(s) 1409, 2102
SmoI CTYRAG 2 cut(s) 1409, 2102
SphI GCATGC 2 cut(s) 1659, 1707
SsiI CCGC 4 cut(s) 386, 1049, 2734, 2786
SspDI GGCGCC 1 cut(s) 225
SspI AATATT 3 cut(s) 1015, 1918, 2583
SspMI CTAG 4 cut(s) 1442, 1848, 2522, 2660
SstI GAGCTC 1 cut(s) 2264
StyD4I CCNGG 5 cut(s) 23, 86, 820, 2255, 2610
StyI CCWWGG 2 cut(s) 302, 2297
TaaI ACNGT 6 cut(s) 181, 558, 625, 877, 2239, 2269
TaiI ACGT 3 cut(s) 48, 780, 1468
TaqI TCGA 4 cut(s) 246, 1670, 2508, 2778
TaqII GACCGA 2 cut(s) 2636, 2686
TatI WGTACW 3 cut(s) 278, 1216, 2664
TauI GCSGC 1 cut(s) 2737
TfiI GAWTC 6 cut(s) 19, 190, 293, 737, 946, 1601
TscAI CASTG 5 cut(s) 807, 1900, 2061, 2244, 2423
TseFI GTSAC 4 cut(s) 1051, 1344, 1368, 2698
Tsp45I GTSAC 4 cut(s) 1051, 1344, 1368, 2698
TspDTI ATGAA 9 cut(s) 972, 1308, 1389, 1407, 1581, 1769, 1866, 2305, 2506
TspGWI ACGGA 3 cut(s) 107, 139, 2859
TspRI CASTG 5 cut(s) 807, 1900, 2061, 2244, 2423
Van91I CCANNNNNTGG 2 cut(s) 1233, 2585
Vha464I CTTAAG 2 cut(s) 1409, 2102
VpaK11BI GGWCC 1 cut(s) 1844
VspI ATTAAT 2 cut(s) 1422, 1863
XapI RAATTY 3 cut(s) 320, 1144, 2636
XceI RCATGY 3 cut(s) 1321, 1659, 1707
XmnI GAANNNNTTC 1 cut(s) 1993
XspI CTAG 4 cut(s) 1442, 1848, 2522, 2660
ZrmI AGTACT 1 cut(s) 280
Zsp2I ATGCAT 1 cut(s) 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.