Rorug04G0222100

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0009386
Physical Location & Seq
Forward (+)
38144706 .. 38150427
5722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0222100.1

Sequence Viewer

Length: 918 bp
ATGGCAATCAGCAACTCCGACAAGCCCACCCTGATGGTCACCAACGACGACGGAATCGACGGCCCTGGACTTCTTGCCCTCGTCCACGTCCTCGTCTCCACCAATCGCTACAGTGTTCTCGTCTGCGCTCCCGACTCGGAAAACTCTGCTGTGAGTCACTGCATCACTTGGCGTCGTCCGATTGCCGCCAAACAAGTTGACATCGCCGGAGCAACGGCTTTTGCTGTTTCTGGGTCTCCAGCTGATTGTGCTTCTTTGGGTGTTTCTAAAGCGCTTGTTCCTTCAGTTCCTGACTTGGTGATCAGCGGCATAAACAAGGGCAGCAACTGTGGTTATCACATTGTTTACTCTGGTACTGTGGCTGGCGCTCGAGAAGCTTTCTTTAATGGTGTACCTGCTCTCTCTGTATCATATGATTGGGTTGAAGGCAAGAGTCACATTAATGACTTCACACTTGCTGCCGAGGCTTGCTTACCTATCATAAATGCAATACTCGTGGAAATAAAAAATCAAACCTTTCCTCAAAGATGTTTTTTGAATGTAGATCTGCCAACAAACGTTGCTACTCATAAGGGGTATAAGCTGACTAAGCAGGGTAACAGTATAATCAAAATGAGGTGGATGCAAGTTACTTCGAAGATGCCAAAAATGTTGTCATCAATGGAAATGGAGAAAGATTCAGCAACATCTACTGGAATTGATACAGCAACTACGCCAAAAGAAGATCCTTTGTTCTCAAGAGAGGTAACTGGTTTCCAACATGATACTGATGATGATACCGACCACAAATGTGTTAAAGAAGGATATATTACTATCACTCCTCTAGGTGCCTTCTCTAATGCTGATGTAGATTGCAGCACCTACTTAAAAGAGTGGCTTCCAAATGTGGCTGAGCTCTCCTCTTCATCTGCCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest

Protein Analysis

305

Amino Acids

32.65

Weight (kDa)

5.22

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SurE PF01975 12 - 202 1.8e-52 Survival protein SurE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 1 cut(s) 827
AciI CCGC 2 cut(s) 186, 306
AclI AACGTT 1 cut(s) 558
AclWI GGATC 1 cut(s) 719
AcuI CTGAAG 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 172
AfaI GTAC 2 cut(s) 355, 393
AfeI AGCGCT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 425, 538
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 1 cut(s) 64
AleI CACNNNNGTG 1 cut(s) 789
AluBI AGCT 4 cut(s) 242, 377, 583, 895
AluI AGCT 4 cut(s) 242, 377, 583, 895
Alw21I GWGCWC 1 cut(s) 897
Alw26I GTCTC 2 cut(s) 100, 240
AlwI GGATC 1 cut(s) 719
AlwNI CAGNNNCTG 2 cut(s) 290, 327
Ama87I CYCGRG 1 cut(s) 369
Aor51HI AGCGCT 1 cut(s) 273
AoxI GGCC 1 cut(s) 61
ApeKI GCWGC 3 cut(s) 321, 458, 855
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
AseI ATTAAT 1 cut(s) 441
AspLEI GCGC 3 cut(s) 128, 274, 368
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 31, 310
AsuII TTCGAA 1 cut(s) 635
AvaI CYCGRG 1 cut(s) 369
BanI GGYRCC 1 cut(s) 827
BanII GRGCYC 1 cut(s) 897
BauI CACGAG 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 897
BbvI GCAGC 3 cut(s) 333, 445, 867
BccI CCATC 1 cut(s) 28
BceAI ACGGC 2 cut(s) 76, 231
BciT130I CCWGG 1 cut(s) 66
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 100, 240
BfaI CTAG 1 cut(s) 824
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 2 cut(s) 275, 369
BfuAI ACCTGC 1 cut(s) 403
BglII AGATCT 1 cut(s) 544
BisI GCNGC 5 cut(s) 186, 307, 322, 459, 856
BlpI GCTNAGC 1 cut(s) 891
BlsI GCNGC 5 cut(s) 187, 308, 323, 460, 857
Bme1390I CCNGG 1 cut(s) 66
BmeT110I CYCGRG 1 cut(s) 369
BmgBI CACGTC 1 cut(s) 88
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 829
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 3 cut(s) 171, 612, 630
BplI GAGNNNNNCTC 2 cut(s) 884, 916
BpmI CTGGAG 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 891
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 721
BsaHI GRCGYC 1 cut(s) 172
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 2 cut(s) 64, 462
BsaXI ACNNNNNCTCC 2 cut(s) 802, 832
Bse1I ACTGG 2 cut(s) 697, 754
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 64, 462
BseGI GGATG 1 cut(s) 627
BseMII CTCAG 1 cut(s) 882
BseNI ACTGG 2 cut(s) 697, 754
BseRI GAGGAG 2 cut(s) 810, 889
BseXI GCAGC 3 cut(s) 333, 445, 867
BshFI GGCC 1 cut(s) 63
BshNI GGYRCC 1 cut(s) 827
BsiHKAI GWGCWC 1 cut(s) 897
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 1 cut(s) 207
BsmAI GTCTC 2 cut(s) 100, 240
BsmBI CGTCTC 1 cut(s) 100
BsnI GGCC 1 cut(s) 63
Bso31I GGTCTC 1 cut(s) 240
BsoBI CYCGRG 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 897
Bsp143I GATC 3 cut(s) 300, 544, 724
Bsp1720I GCTNAGC 1 cut(s) 891
BspACI CCGC 2 cut(s) 186, 306
BspANI GGCC 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 883
BspLI GGNNCC 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 1 cut(s) 719
BspT104I TTCGAA 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 827
BspTNI GGTCTC 1 cut(s) 240
BsrI ACTGG 2 cut(s) 697, 754
BssECI CCNNGG 2 cut(s) 64, 462
BssMI GATC 3 cut(s) 300, 544, 724
BssNI GRCGYC 1 cut(s) 172
BssSI CACGAG 1 cut(s) 494
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 4 cut(s) 113, 329, 358, 602
Bst6I CTCTTC 1 cut(s) 907
BstACI GRCGYC 1 cut(s) 172
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 364, 469
BstDEI CTNAG 2 cut(s) 588, 891
BstEII GGTNACC 1 cut(s) 37
BstF5I GGATG 1 cut(s) 627
BstH2I RGCGCY 2 cut(s) 275, 369
BstHHI GCGC 3 cut(s) 128, 274, 368
BstKTI GATC 3 cut(s) 303, 547, 727
BstMAI GTCTC 2 cut(s) 100, 240
BstMBI GATC 3 cut(s) 300, 544, 724
BstMWI GCNNNNNNNGC 4 cut(s) 248, 374, 464, 589
BstNI CCWGG 1 cut(s) 66
BstPI GGTNACC 1 cut(s) 37
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 1 cut(s) 109
BstV1I GCAGC 3 cut(s) 333, 445, 867
BstX2I RGATCY 2 cut(s) 544, 724
BstXI CCANNNNNNTGG 1 cut(s) 34
BstYI RGATCY 2 cut(s) 544, 724
BsuRI GGCC 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 187
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 1 cut(s) 627
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 2 cut(s) 118, 157
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 2 cut(s) 364, 469
CaiI CAGNNNCTG 2 cut(s) 290, 327
CfoI GCGC 3 cut(s) 128, 274, 368
Cfr13I GGNCC 1 cut(s) 62
CseI GACGC 1 cut(s) 161
Csp6I GTAC 2 cut(s) 354, 392
CspCI CAANNNNNGTGG 2 cut(s) 477, 512
CviAII CATG 1 cut(s) 761
CviQI GTAC 2 cut(s) 354, 392
DdeI CTNAG 2 cut(s) 588, 891
DpnI GATC 3 cut(s) 302, 546, 726
DpnII GATC 3 cut(s) 300, 544, 724
Eam1104I CTCTTC 1 cut(s) 907
EarI CTCTTC 1 cut(s) 907
Ecl136II GAGCTC 1 cut(s) 895
Eco24I GRGCYC 1 cut(s) 897
Eco31I GGTCTC 1 cut(s) 240
Eco47III AGCGCT 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 895
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 369
Eco91I GGTNACC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 895
EcoO65I GGTNACC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 897
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 1 cut(s) 764
FalI AAGNNNNNCTT 2 cut(s) 861, 893
FatI CATG 1 cut(s) 760
FauNDI CATATG 1 cut(s) 412
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 5 cut(s) 186, 307, 322, 459, 856
FokI GGATG 1 cut(s) 634
FriOI GRGCYC 1 cut(s) 897
Fsp4HI GCNGC 5 cut(s) 186, 307, 322, 459, 856
FspBI CTAG 1 cut(s) 824
GlaI GCGC 3 cut(s) 127, 273, 367
GluI GCNGC 5 cut(s) 186, 307, 322, 459, 856
GsuI CTGGAG 1 cut(s) 222
HaeII RGCGCY 2 cut(s) 275, 369
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 161
HhaI GCGC 3 cut(s) 128, 274, 368
Hin1I GRCGYC 1 cut(s) 172
Hin1II CATG 1 cut(s) 764
Hin6I GCGC 3 cut(s) 126, 272, 366
HinP1I GCGC 3 cut(s) 126, 272, 366
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 5 cut(s) 54, 134, 154, 433, 677
HpaII CCGG 1 cut(s) 207
HphI GGTGA 2 cut(s) 31, 310
Hpy166II GTNNAC 4 cut(s) 85, 199, 346, 392
Hpy188I TCNGA 3 cut(s) 19, 139, 180
Hpy188III TCNNGA 4 cut(s) 131, 290, 371, 738
Hpy8I GTNNAC 4 cut(s) 85, 199, 346, 392
Hpy99I CGWCG 4 cut(s) 50, 53, 62, 177
HpyAV CCTTC 4 cut(s) 291, 419, 794, 841
HpyCH4III ACNGT 4 cut(s) 113, 329, 358, 602
HpyCH4IV ACGT 2 cut(s) 87, 558
HpyCH4V TGCA 4 cut(s) 162, 488, 625, 855
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 374, 464, 589
HpyF3I CTNAG 2 cut(s) 588, 891
HpySE526I ACGT 2 cut(s) 87, 558
Hsp92I GRCGYC 1 cut(s) 172
Hsp92II CATG 1 cut(s) 764
HspAI GCGC 3 cut(s) 126, 272, 366
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 3 cut(s) 300, 544, 724
LmnI GCTCC 2 cut(s) 133, 209
Lsp1109I GCAGC 3 cut(s) 333, 445, 867
LweI GCATC 3 cut(s) 171, 612, 630
MaeI CTAG 1 cut(s) 824
MaeII ACGT 2 cut(s) 87, 558
MaeIII GTNAC 6 cut(s) 37, 155, 434, 596, 628, 745
MalI GATC 3 cut(s) 302, 546, 726
MboI GATC 3 cut(s) 300, 544, 724
MboII GAAGA 3 cut(s) 649, 734, 894
MflI RGATCY 2 cut(s) 544, 724
MhlI GDGCHC 1 cut(s) 897
MluCI AATT 1 cut(s) 696
MlyI GAGTC 3 cut(s) 128, 163, 442
MmeI TCCRAC 2 cut(s) 42, 781
MnlI CCTC 8 cut(s) 89, 101, 457, 531, 609, 736, 831, 910
MseI TTAA 4 cut(s) 384, 441, 795, 866
MslI CAYNNNNRTG 3 cut(s) 32, 441, 789
MspA1I CMGCKG 2 cut(s) 242, 306
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 4 cut(s) 248, 374, 464, 589
NdeI CATATG 1 cut(s) 412
NdeII GATC 3 cut(s) 300, 544, 724
NlaIII CATG 1 cut(s) 764
NlaIV GGNNCC 1 cut(s) 829
NmeAIII GCCGAG 1 cut(s) 487
NmuCI GTSAC 3 cut(s) 37, 155, 434
NspV TTCGAA 1 cut(s) 635
OliI CACNNNNGTG 1 cut(s) 789
PaeR7I CTCGAG 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 2 cut(s) 54, 677
PkrI GCNGC 5 cut(s) 187, 308, 323, 460, 857
PleI GAGTC 3 cut(s) 128, 162, 441
PpsI GAGTC 3 cut(s) 128, 162, 441
PshBI ATTAAT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 897
Psp1406I AACGTT 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 64
PspEI GGTNACC 1 cut(s) 37
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 829
PspPI GGNCC 1 cut(s) 62
PstNI CAGNNNCTG 2 cut(s) 290, 327
PsuI RGATCY 2 cut(s) 544, 724
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 2 cut(s) 355, 393
RsaNI GTAC 2 cut(s) 354, 392
RseI CAYNNNNRTG 3 cut(s) 32, 441, 789
SacI GAGCTC 1 cut(s) 897
SaqAI TTAA 4 cut(s) 384, 441, 795, 866
SatI GCNGC 5 cut(s) 186, 307, 322, 459, 856
Sau3AI GATC 3 cut(s) 300, 544, 724
Sau96I GGNCC 1 cut(s) 62
SchI GAGTC 3 cut(s) 128, 163, 442
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 1 cut(s) 897
SfaNI GCATC 3 cut(s) 171, 612, 630
SfcI CTRYAG 1 cut(s) 109
Sfr274I CTCGAG 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 369
SmiMI CAYNNNNRTG 3 cut(s) 32, 441, 789
SmlI CTYRAG 2 cut(s) 369, 736
SmoI CTYRAG 2 cut(s) 369, 736
Sse9I AATT 1 cut(s) 696
SsiI CCGC 2 cut(s) 186, 306
SspMI CTAG 1 cut(s) 824
SstI GAGCTC 1 cut(s) 897
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 4 cut(s) 113, 329, 358, 602
TaiI ACGT 2 cut(s) 90, 561
TaqI TCGA 3 cut(s) 57, 370, 635
TasI AATT 1 cut(s) 696
TauI GCSGC 2 cut(s) 188, 309
TfiI GAWTC 2 cut(s) 54, 677
Tru1I TTAA 4 cut(s) 384, 441, 795, 866
Tru9I TTAA 4 cut(s) 384, 441, 795, 866
TscAI CASTG 2 cut(s) 118, 164
TseFI GTSAC 3 cut(s) 37, 155, 434
TseI GCWGC 3 cut(s) 321, 458, 855
Tsp45I GTSAC 3 cut(s) 37, 155, 434
TspDTI ATGAA 1 cut(s) 894
TspGWI ACGGA 1 cut(s) 66
TspRI CASTG 2 cut(s) 118, 164
VspI ATTAAT 1 cut(s) 441
XhoI CTCGAG 1 cut(s) 369
XspI CTAG 1 cut(s) 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.