Rh4CG300300

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009386
Physical Location & Seq
Reverse (-)
56066217 .. 56073462
7246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG300300.1

Sequence Viewer

Length: 2868 bp
ATGGGGAAAGGAATATGGGTGCACTGGGTCATGATGCTGCTTCACGTCGTCGCCATTCTCATTTTCACCAGAGGCTTTCTCCTCACCCGAACCGAGCTCCCTAACTACAGCAAATGCGACGACGTTTCCCAATCCCCCTGCTTGTTCGAAGACCAAGACCACAAGCCTCACAACCAGAGCCACCCGAACCAGAAGGTTTGCTGGAGCAAGCCCGCTATTGATCGACTCGTCATCATCGTTTTCGACGCCCTGAGGTTTGATTTTGTAGCTCCCAGTACTTTTTTCCAAGAGAAGAAGCCATGGATGAACAAATTGCAACTTATACAACAGCTGGCTGCTACAAATGCATCGTCGGCTCGGATTTTCAAGGCCATTGCTGATCCACCAACCACAAGTTTGCAGCGTTTGAAGGGATTGACAACGGGTGGACTGCCGACTTTTATTGATGTTGGGAATAGCTTTGGTGCTCCAGCAATTGTTGAAGATAACTTGTTACATCAGTTTGCTCAAAATGGAAAGAGAGTTGTCATGATGGGAGATGATACATGGACGCAGTTGTTTCCTAATCATTTTGACAAATCTTTTCCTTACCCATCTTTTAATGTTAGAGATCTTGACACGGTGGACAATGGATGCATTGACCACCTTCTTCCATTCTTGCATCGAGAAGATTGGGATGTCCTTATAGCACACTTTCTTGGTGTGGATCATGCCGGTCACATATTTGGGGTTGATTCTGGCCAAATGATAGAGAAGTTGGAGCAATACAACACTGTTTTGCAAAATGTAGTTGAAGCACTGGAGAGCCAGTCTGCGCCAGGGGGACTACACGAGAATACTTTACTACTTGTGATGGGCGATCATGGACAGACTGTAAATGGAGATCATGGTGGGGGCACTGCTGAAGAGGTTGAGACATCCATGTTTGCCCTGAGTTTTAAGAATCCCCCATCTTCCATACCAGCTGAATTTGATACTTCATTTTGCGGACTAGATTTGGATAACAGGAATATCTGCATCAGTTCCATCCAGCAGCTTGACTTTGCAGTGACGATATCAGCGCTGCTTGGTATTCCTTTTCCTTTTGGAAGTATTGGGCGTGTTAATCCTCAGCTTTATGCTTTAGGAGCTGGAACATGGAATTTTGAAGACAGTGTGGGAAATTGCCAAAATCAGCCAAAGTTGGATGAATGGATGCTGAATTATGTCAATGTACTCTGTACCAATTCTTGGCAGGTAAAAAGATATATAGATATTTATTCAGCTTCTTCAGTTATTGGATTTTCCCATGAAGATTTGTTGCACATAGGAAACATATATGCTAAAGCAGAGGAGAGATGGTCGCATACTACAAAGAAGTTGCTGTTACGTAAAAAGGAAAGCTGCAATGAATTGTTGCCTGCTCTTAAGAGGCAAATTGAGGAGTATTCTGCTTTCCTAGCAAGTGTTGCTGAACTTGCACGGTCCAAATGGACCGAGTTTAATTTAAAGTTGATGGGTGCAGGACTTGGTATTATGGTTGTATCACTTATCATCCATTTCCTTGCAATTAAGAAAGTGAAGGAGCAGTATGGTTTTTCTTTCACTTCATCTGGAGATTCTGGGATTTCCTTGGGGTTAATTTTTGCTTGTTTTGTGGGGGTAATACGTGCATGCAGCTTCCTCTCGAATAGTTTTATCTTGGAAGAGGGAAAAGTGGCATGCTTTCTTTTGGCCACAACTGGATTTGTTAAGTTGCGATCTTCAGTCATGAAGAAGAAGATGATATCAGAAGCATTTGTTTTCCTTCTTCTGGTCACCATTTGCAGATTTACTATTCAAGTTGGATTATCAAAGCATGCTTCTAGTTCTGAATTTGTTAGTGCATATCCTTCGTGGATGCTCGGGATCACCTCGCTTTGGAATTTTGTTGCTGAAGTTTTGCCAGTGCTTGCGCTGATTTTATTGGCATTCTTGCTACACAAGGCCATTACTAGAAGCTCTCCTGAGGGGATTTGGAAGTATATTATTATGGGAACTCATTTAAGTTACATTCTGATAGCAGTGCATTGGGCTGTTGAAAGTAACCTATTGAATCAGGCTTGGGTGCTTCAAGATGTTGGAAGCAATTGCATTCCTTGGCTAGTATATGCCATTGGACTTGGACAACTGTTATTACTGGCAATTAATCAGCTTTTTATTAAACAAAAATCCTTGGAAGGCAGCAAAATCTTATCTGCTAAAGCAGTAGCCATGTTTTCTGTATGGAGTTCAACAGTCATTATTCTGCTAGGAAAACAAGGACCCTTCATTGCGTTAGTATTCATAATTGGAGGTTATTGCATAATGCGGCTGGATAATGTAGAACTGGATGGCAAAGATGGAGGTAGTTGGAATTTGATGCTTGATCCAGTTCCTGTAACACAATGGAGCCTTTTTGCTGTGTGCCTGTTTTTCTGCACTGGCCACTGGTGTGCTTTTGATGGCCTCCGATATGGTGCTGCATTTACAGGCTTTGATGAGTTCATCCTGGTTCCCCAAGCAATCCTTCTTACAATGGATACTTATGGTTTTTCCCTCATCCTTCCAATACTTGGACTTCCATTTCTTGTTGCATGCCGGGGGCAGACCGAAAAAGGAAAGCAACCCATTCTTGGGCGACTATCTCTGGTGTATATGATTTATGGACTCATCGTGGCCACTTCAGTCACAGCCACTATACTATGTGTTACAATCCATAGGCGGCATTTGATGGTATGGGGTTTATTTGCTCCCAAGTTTGTTTTTGATGTGGCGGGTCTTATCCTTACAGACAGTTTCATTTGTTTAGCCTCACTTTACTATTTCAGTCGAGTGGAAGATCATGCCCTATACGATCCATTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest

Protein Analysis

955

Amino Acids

106.2

Weight (kDa)

6.26

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphodiest PF01663 212 - 292 1.8e-09 Type I phosphodiesterase / nucleotide pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1225
AccB7I CCANNNNNTGG 2 cut(s) 1230, 2573
AciI CCGC 5 cut(s) 213, 987, 2329, 2722, 2774
AclWI GGATC 5 cut(s) 374, 714, 1895, 2381, 2849
AcoI YGGCCR 4 cut(s) 739, 1713, 2443, 2676
AcsI RAATTY 5 cut(s) 968, 1141, 1853, 1903, 2374
AcuI CTGAAG 5 cut(s) 924, 1254, 1728, 1935, 2667
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 3 cut(s) 277, 1215, 1222
AfeI AGCGCT 1 cut(s) 1062
AfiI CCNNNNNNNGG 6 cut(s) 1230, 1792, 1899, 2573, 2633, 2634
AflII CTTAAG 1 cut(s) 1406
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 817, 2508
AjuI GAANNNNNNNTTGG 2 cut(s) 2511, 2543
AleI CACNNNNGTG 1 cut(s) 2451
Alw21I GWGCWC 3 cut(s) 24, 99, 469
Alw26I GTCTC 1 cut(s) 908
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 5 cut(s) 374, 714, 1895, 2381, 2849
AlwNI CAGNNNCTG 1 cut(s) 2396
Ama87I CYCGRG 1 cut(s) 1883
Aor51HI AGCGCT 1 cut(s) 1062
AoxI GGCC 7 cut(s) 369, 739, 1713, 1965, 2443, 2464, 2676
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 9 cut(s) 37, 335, 400, 1033, 1063, 1383, 1656, 2202, 2480
ApoI RAATTY 5 cut(s) 968, 1141, 1853, 1903, 2374
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AseI ATTAAT 1 cut(s) 2166
AspLEI GCGC 3 cut(s) 817, 1063, 1936
AspS9I GGNCC 3 cut(s) 1464, 1473, 2282
AsuC2I CCSGG 1 cut(s) 2600
AsuHPI GGTGA 4 cut(s) 58, 76, 1789, 1882
AsuII TTCGAA 1 cut(s) 147
AvaI CYCGRG 1 cut(s) 1883
AvaII GGWCC 3 cut(s) 1464, 1473, 2282
AxyI CCTNAGG 2 cut(s) 251, 1986
BaeGI GKGCMC 2 cut(s) 24, 899
BalI TGGCCA 4 cut(s) 741, 1715, 2445, 2678
BanII GRGCYC 1 cut(s) 99
BauI CACGAG 1 cut(s) 830
BbsI GAAGAC 2 cut(s) 156, 1155
Bbv12I GWGCWC 3 cut(s) 24, 99, 469
BbvCI CCTCAGC 1 cut(s) 1110
BbvI GCAGC 9 cut(s) 24, 322, 412, 1045, 1050, 1370, 1668, 2214, 2467
BcgI CGANNNNNNTGC 2 cut(s) 1854, 1888
BciT130I CCWGG 2 cut(s) 819, 2510
BciVI GTATCC 1 cut(s) 2533
BcnI CCSGG 1 cut(s) 2600
BcoDI GTCTC 1 cut(s) 908
BfaI CTAG 6 cut(s) 992, 1439, 1845, 1974, 2123, 2270
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 1064
BfrI CTTAAG 1 cut(s) 1406
BfuAI ACCTGC 1 cut(s) 1225
BfuI GTATCC 1 cut(s) 2533
BglII AGATCT 1 cut(s) 610
BmcAI AGTACT 1 cut(s) 277
Bme1390I CCNGG 3 cut(s) 819, 2510, 2600
Bme18I GGWCC 3 cut(s) 1464, 1473, 2282
BmeT110I CYCGRG 1 cut(s) 1883
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 1464, 1473, 2282
BmiI GGNNCC 3 cut(s) 2284, 2411, 2514
BmrFI CCNGG 3 cut(s) 819, 2510, 2600
BmrI ACTGGG 2 cut(s) 34, 267
BmsI GCATC 8 cut(s) 24, 356, 623, 670, 1026, 1185, 1869, 2370
BmuI ACTGGG 2 cut(s) 34, 267
BpiI GAAGAC 2 cut(s) 156, 1155
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpmI CTGGAG 4 cut(s) 223, 453, 821, 1614
Bpu10I CCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 2600
BsaAI YACGTR 2 cut(s) 1370, 1649
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 6 cut(s) 299, 818, 1611, 2117, 2193, 2599
BsaXI ACNNNNNCTCC 4 cut(s) 794, 824, 1325, 1355
Bsc4I CCNNNNNNNGG 6 cut(s) 1230, 1792, 1899, 2573, 2633, 2634
Bse118I RCCGGY 1 cut(s) 713
Bse21I CCTNAGG 2 cut(s) 251, 1986
Bse3DI GCAATG 3 cut(s) 372, 1393, 2289
BseBI CCWGG 2 cut(s) 819, 2510
BseDI CCNNGG 6 cut(s) 299, 818, 1611, 2117, 2193, 2599
BseLI CCNNNNNNNGG 6 cut(s) 1230, 1792, 1899, 2573, 2633, 2634
BseMI GCAATG 3 cut(s) 372, 1393, 2289
BseMII CTCAG 4 cut(s) 242, 923, 1124, 1977
BseRI GAGGAG 3 cut(s) 71, 1346, 1436
BseSI GKGCMC 2 cut(s) 24, 899
BseXI GCAGC 9 cut(s) 24, 322, 412, 1045, 1050, 1370, 1668, 2214, 2467
BsgI GTGCAG 2 cut(s) 1521, 2422
BshFI GGCC 7 cut(s) 371, 741, 1715, 1967, 2445, 2466, 2678
BsiHKAI GWGCWC 3 cut(s) 24, 99, 469
BsiHKCI CYCGRG 1 cut(s) 1883
BsiSI CCGG 2 cut(s) 714, 2599
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 6 cut(s) 1230, 1792, 1899, 2573, 2633, 2634
BsmAI GTCTC 1 cut(s) 908
BsmFI GGGAC 1 cut(s) 837
BsmI GAATGC 2 cut(s) 1949, 2112
BsnI GGCC 7 cut(s) 371, 741, 1715, 1967, 2445, 2466, 2678
BsoBI CYCGRG 1 cut(s) 1883
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 4 cut(s) 24, 99, 469, 899
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 5 cut(s) 213, 987, 2329, 2722, 2774
BspANI GGCC 7 cut(s) 371, 741, 1715, 1967, 2445, 2466, 2678
BspCNI CTCAG 4 cut(s) 243, 924, 1123, 1978
BspHI TCATGA 3 cut(s) 30, 528, 1749
BspLI GGNNCC 3 cut(s) 2284, 2411, 2514
BspMI ACCTGC 1 cut(s) 1225
BspPI GGATC 5 cut(s) 374, 714, 1895, 2381, 2849
BspT104I TTCGAA 1 cut(s) 147
BspTI CTTAAG 1 cut(s) 1406
BsrDI GCAATG 3 cut(s) 372, 1393, 2289
BsrFI RCCGGY 1 cut(s) 713
BssAI RCCGGY 1 cut(s) 713
BssECI CCNNGG 6 cut(s) 299, 818, 1611, 2117, 2193, 2599
BssNI GRCGYC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 830
BssT1I CCWWGG 4 cut(s) 299, 1611, 2117, 2193
Bst2BI CACGAG 1 cut(s) 830
Bst2UI CCWGG 2 cut(s) 819, 2510
Bst4CI ACNGT 8 cut(s) 622, 775, 874, 1154, 1464, 2151, 2257, 2795
Bst6I CTCTTC 2 cut(s) 900, 1680
BstACI GRCGYC 1 cut(s) 246
BstAFI CTTAAG 1 cut(s) 1406
BstAPI GCANNNNNTGC 1 cut(s) 1448
BstBAI YACGTR 2 cut(s) 1370, 1649
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 9 cut(s) 209, 213, 333, 1401, 1654, 1702, 1839, 1932, 2596
BstDEI CTNAG 4 cut(s) 251, 932, 1110, 1986
BstDSI CCRYGG 1 cut(s) 299
BstEII GGTNACC 1 cut(s) 1795
BstH2I RGCGCY 1 cut(s) 1064
BstHHI GCGC 3 cut(s) 817, 1063, 1936
BstMAI GTCTC 1 cut(s) 908
BstMWI GCNNNNNNNGC 6 cut(s) 344, 353, 1127, 1439, 1448, 1457
BstNI CCWGG 2 cut(s) 819, 2510
BstNSI RCATGY 4 cut(s) 1656, 1704, 1841, 2598
BstPI GGTNACC 1 cut(s) 1795
BstSCI CCNGG 3 cut(s) 817, 2508, 2598
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 2 cut(s) 24, 899
BstSNI TACGTA 1 cut(s) 1370
BstV1I GCAGC 9 cut(s) 24, 322, 412, 1045, 1050, 1370, 1668, 2214, 2467
BstV2I GAAGAC 2 cut(s) 156, 1155
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
Bsu36I CCTNAGG 2 cut(s) 251, 1986
BsuI GTATCC 1 cut(s) 2533
BsuRI GGCC 7 cut(s) 371, 741, 1715, 1967, 2445, 2466, 2678
BtgI CCRYGG 1 cut(s) 299
BtrI CACGTC 1 cut(s) 46
BtsI GCAGTG 3 cut(s) 897, 1053, 2049
BveI ACCTGC 1 cut(s) 1225
Cac8I GCNNGC 9 cut(s) 209, 213, 333, 1401, 1654, 1702, 1839, 1932, 2596
CaiI CAGNNNCTG 1 cut(s) 2396
CciI TCATGA 3 cut(s) 30, 528, 1749
CfoI GCGC 3 cut(s) 817, 1063, 1936
Cfr10I RCCGGY 1 cut(s) 713
Cfr13I GGNCC 3 cut(s) 1464, 1473, 2282
CseI GACGC 2 cut(s) 254, 559
Csp6I GTAC 3 cut(s) 276, 1214, 1221
CviQI GTAC 3 cut(s) 276, 1214, 1221
DdeI CTNAG 4 cut(s) 251, 932, 1110, 1986
DraI TTTAAA 1 cut(s) 1488
EaeI YGGCCR 4 cut(s) 739, 1713, 2443, 2676
Eam1104I CTCTTC 2 cut(s) 900, 1680
EarI CTCTTC 2 cut(s) 900, 1680
Ecl136II GAGCTC 1 cut(s) 97
Eco105I TACGTA 1 cut(s) 1370
Eco130I CCWWGG 4 cut(s) 299, 1611, 2117, 2193
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 2 cut(s) 1056, 1767
Eco47I GGWCC 3 cut(s) 1464, 1473, 2282
Eco47III AGCGCT 1 cut(s) 1062
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 5 cut(s) 924, 1254, 1728, 1935, 2667
Eco81I CCTNAGG 2 cut(s) 251, 1986
Eco88I CYCGRG 1 cut(s) 1883
Eco91I GGTNACC 1 cut(s) 1795
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 2282
EcoO65I GGTNACC 1 cut(s) 1795
EcoRII CCWGG 2 cut(s) 817, 2508
EcoRV GATATC 2 cut(s) 1056, 1767
EcoT14I CCWWGG 4 cut(s) 299, 1611, 2117, 2193
EcoT22I ATGCAT 2 cut(s) 349, 638
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 4 cut(s) 299, 1611, 2117, 2193
FalI AAGNNNNNCTT 2 cut(s) 2511, 2543
FaqI GGGAC 1 cut(s) 837
FauI CCCGC 2 cut(s) 220, 2767
FriOI GRGCYC 1 cut(s) 99
FspBI CTAG 6 cut(s) 992, 1439, 1845, 1974, 2123, 2270
GlaI GCGC 3 cut(s) 816, 1062, 1935
GsuI CTGGAG 4 cut(s) 223, 453, 821, 1614
HaeII RGCGCY 1 cut(s) 1064
HaeIII GGCC 7 cut(s) 371, 741, 1715, 1967, 2445, 2466, 2678
HapII CCGG 2 cut(s) 714, 2599
HgaI GACGC 2 cut(s) 254, 559
HhaI GCGC 3 cut(s) 817, 1063, 1936
Hin1I GRCGYC 1 cut(s) 246
Hin6I GCGC 3 cut(s) 815, 1061, 1934
HinP1I GCGC 3 cut(s) 815, 1061, 1934
HinfI GANTC 6 cut(s) 225, 734, 943, 1598, 2074, 2667
HpaII CCGG 2 cut(s) 714, 2599
HphI GGTGA 4 cut(s) 58, 76, 1789, 1882
Hpy166II GTNNAC 3 cut(s) 22, 428, 625
Hpy188I TCNGA 5 cut(s) 360, 1771, 1852, 2037, 2471
Hpy8I GTNNAC 3 cut(s) 22, 428, 625
Hpy99I CGWCG 6 cut(s) 50, 53, 122, 125, 248, 355
HpyCH4III ACNGT 8 cut(s) 622, 775, 874, 1154, 1464, 2151, 2257, 2795
HpyCH4IV ACGT 4 cut(s) 45, 123, 1369, 1648
HpyF10VI GCNNNNNNNGC 6 cut(s) 344, 353, 1127, 1439, 1448, 1457
HpyF3I CTNAG 4 cut(s) 251, 932, 1110, 1986
HpySE526I ACGT 4 cut(s) 45, 123, 1369, 1648
Hsp92I GRCGYC 1 cut(s) 246
HspAI GCGC 3 cut(s) 815, 1061, 1934
LmnI GCTCC 9 cut(s) 102, 204, 274, 472, 760, 1127, 1564, 2409, 2755
Lsp1109I GCAGC 9 cut(s) 24, 322, 412, 1045, 1050, 1370, 1668, 2214, 2467
LweI GCATC 8 cut(s) 24, 356, 623, 670, 1026, 1185, 1869, 2370
MaeI CTAG 6 cut(s) 992, 1439, 1845, 1974, 2123, 2270
MaeII ACGT 4 cut(s) 45, 123, 1369, 1648
MfeI CAATTG 2 cut(s) 474, 2107
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 4 cut(s) 24, 99, 469, 899
MlsI TGGCCA 4 cut(s) 741, 1715, 2445, 2678
MluNI TGGCCA 4 cut(s) 741, 1715, 2445, 2678
MlyI GAGTC 2 cut(s) 219, 2661
MmeI TCCRAC 5 cut(s) 738, 1164, 1804, 2080, 2351
Mox20I TGGCCA 4 cut(s) 741, 1715, 2445, 2678
Mph1103I ATGCAT 2 cut(s) 349, 638
MscI TGGCCA 4 cut(s) 741, 1715, 2445, 2678
MslI CAYNNNNRTG 1 cut(s) 2451
Msp20I TGGCCA 4 cut(s) 741, 1715, 2445, 2678
MspA1I CMGCKG 2 cut(s) 331, 965
MspCI CTTAAG 1 cut(s) 1406
MspI CCGG 2 cut(s) 714, 2599
MspR9I CCNGG 3 cut(s) 819, 2510, 2600
MunI CAATTG 2 cut(s) 474, 2107
Mva1269I GAATGC 2 cut(s) 1949, 2112
MvaI CCWGG 2 cut(s) 819, 2510
MwoI GCNNNNNNNGC 6 cut(s) 344, 353, 1127, 1439, 1448, 1457
NciI CCSGG 1 cut(s) 2600
NcoI CCATGG 1 cut(s) 299
NlaIV GGNNCC 3 cut(s) 2284, 2411, 2514
NmuCI GTSAC 4 cut(s) 716, 1048, 1795, 2686
NsiI ATGCAT 2 cut(s) 349, 638
NspI RCATGY 4 cut(s) 1656, 1704, 1841, 2598
NspV TTCGAA 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 2451
PaeI GCATGC 4 cut(s) 1656, 1704, 1841, 2598
PagI TCATGA 3 cut(s) 30, 528, 1749
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PctI GAATGC 2 cut(s) 1949, 2112
PfeI GAWTC 4 cut(s) 734, 943, 1598, 2074
PflMI CCANNNNNTGG 2 cut(s) 1230, 2573
PleI GAGTC 2 cut(s) 219, 2661
PpsI GAGTC 2 cut(s) 219, 2661
Ppu21I YACGTR 2 cut(s) 1370, 1649
PpuMI RGGWCCY 1 cut(s) 2282
PshBI ATTAAT 1 cut(s) 2166
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 2282
Psp6I CCWGG 2 cut(s) 817, 2508
PspEI GGTNACC 1 cut(s) 1795
PspGI CCWGG 2 cut(s) 817, 2508
PspN4I GGNNCC 3 cut(s) 2284, 2411, 2514
PspPI GGNCC 3 cut(s) 1464, 1473, 2282
PspPPI RGGWCCY 1 cut(s) 2282
PstNI CAGNNNCTG 1 cut(s) 2396
PsuI RGATCY 1 cut(s) 610
PvuII CAGCTG 2 cut(s) 331, 965
RsaI GTAC 3 cut(s) 277, 1215, 1222
RsaNI GTAC 3 cut(s) 276, 1214, 1221
RseI CAYNNNNRTG 1 cut(s) 2451
SacI GAGCTC 1 cut(s) 99
Sau96I GGNCC 3 cut(s) 1464, 1473, 2282
ScaI AGTACT 1 cut(s) 277
SchI GAGTC 2 cut(s) 219, 2661
ScrFI CCNGG 3 cut(s) 819, 2510, 2600
SduI GDGCHC 4 cut(s) 24, 99, 469, 899
SfaNI GCATC 8 cut(s) 24, 356, 623, 670, 1026, 1185, 1869, 2370
SfcI CTRYAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 3 cut(s) 1464, 1473, 2282
SmiMI CAYNNNNRTG 1 cut(s) 2451
SmlI CTYRAG 1 cut(s) 1406
SmoI CTYRAG 1 cut(s) 1406
SnaBI TACGTA 1 cut(s) 1370
SphI GCATGC 4 cut(s) 1656, 1704, 1841, 2598
SsiI CCGC 5 cut(s) 213, 987, 2329, 2722, 2774
SspMI CTAG 6 cut(s) 992, 1439, 1845, 1974, 2123, 2270
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 3 cut(s) 817, 2508, 2598
StyI CCWWGG 4 cut(s) 299, 1611, 2117, 2193
TaaI ACNGT 8 cut(s) 622, 775, 874, 1154, 1464, 2151, 2257, 2795
TaiI ACGT 4 cut(s) 48, 126, 1372, 1651
TaqI TCGA 6 cut(s) 147, 223, 243, 664, 1667, 2830
TaqII GACCGA 2 cut(s) 1490, 2624
TatI WGTACW 2 cut(s) 275, 1213
TauI GCSGC 2 cut(s) 2332, 2725
TfiI GAWTC 4 cut(s) 734, 943, 1598, 2074
TseFI GTSAC 4 cut(s) 716, 1048, 1795, 2686
TseI GCWGC 9 cut(s) 37, 335, 400, 1033, 1063, 1383, 1656, 2202, 2480
Tsp45I GTSAC 4 cut(s) 716, 1048, 1795, 2686
Van91I CCANNNNNTGG 2 cut(s) 1230, 2573
Vha464I CTTAAG 1 cut(s) 1406
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 3 cut(s) 1464, 1473, 2282
VspI ATTAAT 1 cut(s) 2166
XapI RAATTY 5 cut(s) 968, 1141, 1853, 1903, 2374
XceI RCATGY 4 cut(s) 1656, 1704, 1841, 2598
XspI CTAG 6 cut(s) 992, 1439, 1845, 1974, 2123, 2270
ZrmI AGTACT 1 cut(s) 277
Zsp2I ATGCAT 2 cut(s) 349, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.