Rroxscaffold_5G00369780

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009386
Physical Location & Seq
Reverse (-)
51571318 .. 51578762
7445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00369780.1

Sequence Viewer

Length: 2865 bp
ATGGGGAAAGGAATATGTGTGCACTGGGTCATGACGCTGCTTCACGTCGTCGCCATTCTCATTTTCACCAGAGGCTTTCTCCTCACCCGAACCGAGCTCCCTAACTACAGCAAATGCGACGACGTTTCCCAATCCCCCTGCTTCTTCGAAGACCAAGACCACAAGCCTCACAACCAGAGCCAGAATCAGAAGGTTTGCTGGAGCAAACCCGCTGTTGATCGACTCGTCATCATCGTTTTCGACGCCCTGAGGTTTGATTTTGTAGCTCCCAGTACTTTTTTTCAAGAGAAGAAGCCATGGATGAACAAATTGCAACTTATACAACAGCTGGCTGCTACAAATGCATCGTCGGCTCGGATTTTCAAGGCCATTGCTGATCCACCAACCACAAGTTTGCAGCGTTTGAAGGGATTGACAACGGGTGGACTGCCGACTTTTATTGATGTTGGGAATAGCTTTGGTGCTCCAGCAATTGTTGAAGATAACTTGTTACATCAGTTGGCTCAAAATGGAAAGAGAGTTGTCATGATGGGAGATGATACATGGACGCAGTTGTTTCCTAATCATTTTGACAAATCTTTTCCTTACCCATCTTTTAATGTTAGAGATCTTGACACGGTGGACAACGGATGCATTGACCACCTTCTTCCATTCTTGCATCGAGAAGATTGGGATGTCCTTATAGCACACTTTCTTGGTGTGGATCATGCCGGTCACATATTTGGGGTTGATTCTGGCCAAATGATAGAGAAGTTGGAGCAATACAACACCGTTTTGCAAAATGTAGTTGAAGCACTGGAGAGCCAGTCTGCGCCAGGGGGACTACACGAGAATACTTTACTACTTGTGATGGGTGATCATGGACAGACTGTAAATGGAGATCATGGTGGGGGCACTGCTGAAGAGGTTGAGACATCCATGTTTGCCCTGAGTTTTAAGAATCCCCCATCTCCCATACCAGCTGAATTTGATACTTCATTTTGCGGACTAGATTTGGATAAGAGGAATATCTGCATCAGTTCCATCCACCAGCTTGACTTTGCAGTGACGATATCAGCGCTGCTTGGTATTCCCTTTCCTTTTGGCAGTATTGGGCGTGTTAATCCTCAGCTTTATGCTTTAGGAGCTGGAACATGGAATTTTGAAGACAGTGTGGGAAATTGCCAAAATCAGCCAAAGTTGGATGAATGGATGCTGAATTATGTCAATATACTCTGTACCAATTCTTGGCAGGTAAAAAGATACATAGATATTTATTCAGCTTCTTCAGTTATTGGATTTTCCCATGAAGATTTGTTGCACATAGGAAACATATACGCTAAAGCAGAGGAGAGATGGTCACATACTACAAAGAAGTTGCTGTTACATAAAAAGGAAAGCTGCAATGAATTGTTGCCTGCTCTTAAGAGGCAAATTGAGGAGTATTCTGCTTTCCTAGCAAGTGTTGCTGAACTTGCACGGTCCAAATGGACCGAGTTTAATTTAAAGTTGATGGGTGCAGGACTTGGTGTTATGGTTGTATCACTTATCATCCATTTCCTTGCAATTAAGAAAGTAAAGGAGCAGTATGGTTTTTCTTTCACTTCATCTGGAGATTCTGGGATTTCCTTGCGGTTAATTTTTGCTTGTTTTGTGGGGGTAATACGTGCATGCAGCTTCCTCTCAAATAGTTTTATCTTGGAAGAGGGAAAAGTGGCATGCTTTCTTTTGGCCACAACTGGATTTGTTAAGTTGCGATCTTCAGTCATGAAGAAGAAGATGATATCAGAAGCATTTGTTTTCCTTCTTCTGGTCACCATTTGCAGATTTACTATTGAAGTTGGATTATCAAAGCATGCTTCTAGTTCTGAATTTGTGAGTGCATATCCTTCGTGGATGCTTGGGATCACCTCGCTTTGGAATTTTGTTGCTGAAGTTTTGCCAGTGCTTGCGCTGATTTTATTGGCATTCTTGCTACACAAGGCCATTACTAGAAGCTCTCCTGAGGGGATTTGGAAGTATATTATTATGGGAACTCATTTAAGTTACGTTCTGATAGCAGTGCATTGGGCTGTTGAAAGTAACCTATTGAATCTGGCTTGGGTGCTTAAAGATGTTGGAAGCAATTGCATTCCTCGGCTAGTATATGCCATTGGACTTGGACAACTGTTATTACTGGCAATTAATCAGCTTTTTATTAAACAAAAATCCTTGAAAGGCAGCAAAATCTTATCTGCTAAAGCAGTAGCCATGTTTTCTGTATGGAGTTCAACAGTCATTATTCTGCTAGGAAAACAAGGACCCTTCATTGCGTTAGTATTCATAATTGGAGGTTATTGCATCATGCGGCTGGATAATGTAGAACTGGATGGCAAAGATGGAGGTAGTTGGAATTTGATGCTTGATCCAGTTCCTGTAACACAATGGAGCCTTTTTGCTGTGTGCCTGTTTTTCTGCACTGGCCACTGGTGTGCTTTTGATGGCCTCCGATATGGTGCTGCATTTACAGGCTTTGATGAGTTCATCCTGGTTCCCCAAGCAATCCTTCTTACAATGGATACTTATGGTTTTTCCCTCATCCTTCCAATACTTGGACTTCCATTTCTTGTTGCATGCCGGGGGCAGACCGAAAAAGGCAAGCAGCCCATTCTTGGGCGACTATCTCTGGTGTATATGATTTATGGACTCATTGTGGCCACTTCAGTCACAGCCACTATACTATGTGTTACAATCCATAGGCGGCATTTGATGGTATGGGGTTTATTTGCTCCCAAGTTTGTTTTTGATGTGGCGGGTCTTATCCTCACAGACAGTTTCATTTGTTTAGCCTCACTTTACTATTTCAGTCGAGTGGAAGATCATGCCCGATACGATCCATTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest

Protein Analysis

954

Amino Acids

106.06

Weight (kDa)

6.64

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphodiest PF01663 211 - 291 1.8e-09 Type I phosphodiesterase / nucleotide pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1222
AccB7I CCANNNNNTGG 2 cut(s) 1227, 2570
AciI CCGC 6 cut(s) 210, 984, 1612, 2326, 2719, 2771
AclWI GGATC 5 cut(s) 371, 711, 1892, 2378, 2846
AcoI YGGCCR 4 cut(s) 736, 1710, 2440, 2673
AcsI RAATTY 5 cut(s) 965, 1138, 1850, 1900, 2371
AcuI CTGAAG 5 cut(s) 921, 1251, 1725, 1932, 2664
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 274, 1219
AfeI AGCGCT 1 cut(s) 1059
AfiI CCNNNNNNNGG 6 cut(s) 1227, 1789, 1896, 2570, 2630, 2631
AflII CTTAAG 1 cut(s) 1403
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 814, 2505
AjuI GAANNNNNNNTTGG 2 cut(s) 2508, 2540
AleI CACNNNNGTG 1 cut(s) 2448
Alw21I GWGCWC 3 cut(s) 24, 99, 466
Alw26I GTCTC 1 cut(s) 905
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 5 cut(s) 371, 711, 1892, 2378, 2846
AlwNI CAGNNNCTG 1 cut(s) 2393
Aor51HI AGCGCT 1 cut(s) 1059
AoxI GGCC 7 cut(s) 366, 736, 1710, 1962, 2440, 2461, 2673
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 9 cut(s) 37, 332, 397, 1060, 1380, 1653, 2199, 2477, 2620
ApoI RAATTY 5 cut(s) 965, 1138, 1850, 1900, 2371
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AseI ATTAAT 1 cut(s) 2163
AspLEI GCGC 3 cut(s) 814, 1060, 1933
AspS9I GGNCC 3 cut(s) 1461, 1470, 2279
AsuC2I CCSGG 1 cut(s) 2597
AsuHPI GGTGA 5 cut(s) 58, 76, 866, 1786, 1879
AsuII TTCGAA 1 cut(s) 147
AvaII GGWCC 3 cut(s) 1461, 1470, 2279
AxyI CCTNAGG 2 cut(s) 248, 1983
BaeGI GKGCMC 2 cut(s) 24, 896
BalI TGGCCA 4 cut(s) 738, 1712, 2442, 2675
BanII GRGCYC 1 cut(s) 99
BauI CACGAG 1 cut(s) 827
BbsI GAAGAC 2 cut(s) 156, 1152
Bbv12I GWGCWC 3 cut(s) 24, 99, 466
BbvCI CCTCAGC 1 cut(s) 1107
BbvI GCAGC 9 cut(s) 24, 319, 409, 1047, 1367, 1665, 2211, 2464, 2632
BcgI CGANNNNNNTGC 2 cut(s) 1851, 1885
BciT130I CCWGG 2 cut(s) 816, 2507
BciVI GTATCC 1 cut(s) 2530
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 1 cut(s) 2597
BcoDI GTCTC 1 cut(s) 905
BfaI CTAG 6 cut(s) 989, 1436, 1842, 1971, 2120, 2267
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 1061
BfrI CTTAAG 1 cut(s) 1403
BfuAI ACCTGC 1 cut(s) 1222
BfuI GTATCC 1 cut(s) 2530
BglII AGATCT 1 cut(s) 607
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 3 cut(s) 816, 2507, 2597
Bme18I GGWCC 3 cut(s) 1461, 1470, 2279
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 1461, 1470, 2279
BmiI GGNNCC 3 cut(s) 2281, 2408, 2511
BmrFI CCNGG 3 cut(s) 816, 2507, 2597
BmrI ACTGGG 2 cut(s) 34, 264
BmsI GCATC 8 cut(s) 353, 620, 667, 1023, 1182, 1866, 2328, 2367
BmuI ACTGGG 2 cut(s) 34, 264
BpiI GAAGAC 2 cut(s) 156, 1152
BplI GAGNNNNNCTC 2 cut(s) 63, 95
BpmI CTGGAG 4 cut(s) 220, 450, 818, 1611
Bpu10I CCTNAGC 1 cut(s) 1107
Bpu14I TTCGAA 1 cut(s) 147
BpuMI CCSGG 1 cut(s) 2597
BsaAI YACGTR 1 cut(s) 1646
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 4 cut(s) 296, 815, 2114, 2596
BsaXI ACNNNNNCTCC 4 cut(s) 791, 821, 1322, 1352
Bsc4I CCNNNNNNNGG 6 cut(s) 1227, 1789, 1896, 2570, 2630, 2631
Bse118I RCCGGY 1 cut(s) 710
Bse21I CCTNAGG 2 cut(s) 248, 1983
Bse3DI GCAATG 3 cut(s) 369, 1390, 2286
BseBI CCWGG 2 cut(s) 816, 2507
BseDI CCNNGG 4 cut(s) 296, 815, 2114, 2596
BseLI CCNNNNNNNGG 6 cut(s) 1227, 1789, 1896, 2570, 2630, 2631
BseMI GCAATG 3 cut(s) 369, 1390, 2286
BseMII CTCAG 4 cut(s) 239, 920, 1121, 1974
BseRI GAGGAG 3 cut(s) 71, 1343, 1433
BseSI GKGCMC 2 cut(s) 24, 896
BseXI GCAGC 9 cut(s) 24, 319, 409, 1047, 1367, 1665, 2211, 2464, 2632
BsgI GTGCAG 2 cut(s) 1518, 2419
BshFI GGCC 7 cut(s) 368, 738, 1712, 1964, 2442, 2463, 2675
BsiHKAI GWGCWC 3 cut(s) 24, 99, 466
BsiSI CCGG 2 cut(s) 711, 2596
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 6 cut(s) 1227, 1789, 1896, 2570, 2630, 2631
BsmAI GTCTC 1 cut(s) 905
BsmFI GGGAC 1 cut(s) 834
BsmI GAATGC 2 cut(s) 1946, 2109
BsnI GGCC 7 cut(s) 368, 738, 1712, 1964, 2442, 2463, 2675
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 4 cut(s) 24, 99, 466, 896
Bsp19I CCATGG 1 cut(s) 296
BspACI CCGC 6 cut(s) 210, 984, 1612, 2326, 2719, 2771
BspANI GGCC 7 cut(s) 368, 738, 1712, 1964, 2442, 2463, 2675
BspCNI CTCAG 4 cut(s) 240, 921, 1120, 1975
BspHI TCATGA 3 cut(s) 30, 525, 1746
BspLI GGNNCC 3 cut(s) 2281, 2408, 2511
BspMI ACCTGC 1 cut(s) 1222
BspPI GGATC 5 cut(s) 371, 711, 1892, 2378, 2846
BspT104I TTCGAA 1 cut(s) 147
BspTI CTTAAG 1 cut(s) 1403
BsrDI GCAATG 3 cut(s) 369, 1390, 2286
BsrFI RCCGGY 1 cut(s) 710
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 4 cut(s) 296, 815, 2114, 2596
BssNI GRCGYC 1 cut(s) 243
BssSI CACGAG 1 cut(s) 827
BssT1I CCWWGG 1 cut(s) 296
Bst2BI CACGAG 1 cut(s) 827
Bst2UI CCWGG 2 cut(s) 816, 2507
Bst4CI ACNGT 8 cut(s) 619, 772, 871, 1151, 1461, 2148, 2254, 2792
Bst6I CTCTTC 2 cut(s) 897, 1677
BstACI GRCGYC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 1403
BstAPI GCANNNNNTGC 1 cut(s) 1445
BstBAI YACGTR 1 cut(s) 1646
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 8 cut(s) 330, 1398, 1651, 1699, 1836, 1929, 2593, 2618
BstDEI CTNAG 4 cut(s) 248, 929, 1107, 1983
BstDSI CCRYGG 1 cut(s) 296
BstEII GGTNACC 1 cut(s) 1792
BstH2I RGCGCY 1 cut(s) 1061
BstHHI GCGC 3 cut(s) 814, 1060, 1933
BstMAI GTCTC 1 cut(s) 905
BstMWI GCNNNNNNNGC 6 cut(s) 341, 350, 1124, 1436, 1445, 1454
BstNI CCWGG 2 cut(s) 816, 2507
BstNSI RCATGY 4 cut(s) 1653, 1701, 1838, 2595
BstPI GGTNACC 1 cut(s) 1792
BstSCI CCNGG 3 cut(s) 814, 2505, 2595
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 2 cut(s) 24, 896
BstV1I GCAGC 9 cut(s) 24, 319, 409, 1047, 1367, 1665, 2211, 2464, 2632
BstV2I GAAGAC 2 cut(s) 156, 1152
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
Bsu36I CCTNAGG 2 cut(s) 248, 1983
BsuI GTATCC 1 cut(s) 2530
BsuRI GGCC 7 cut(s) 368, 738, 1712, 1964, 2442, 2463, 2675
BtgI CCRYGG 1 cut(s) 296
BtrI CACGTC 1 cut(s) 46
BtsI GCAGTG 3 cut(s) 894, 1050, 2046
BtsIMutI CAGTG 9 cut(s) 22, 794, 894, 1050, 1156, 1929, 2046, 2436, 2443
BveI ACCTGC 1 cut(s) 1222
Cac8I GCNNGC 8 cut(s) 330, 1398, 1651, 1699, 1836, 1929, 2593, 2618
CaiI CAGNNNCTG 1 cut(s) 2393
CciI TCATGA 3 cut(s) 30, 525, 1746
CfoI GCGC 3 cut(s) 814, 1060, 1933
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 3 cut(s) 1461, 1470, 2279
CseI GACGC 3 cut(s) 43, 251, 556
Csp6I GTAC 2 cut(s) 273, 1218
CviQI GTAC 2 cut(s) 273, 1218
DdeI CTNAG 4 cut(s) 248, 929, 1107, 1983
DraI TTTAAA 1 cut(s) 1485
EaeI YGGCCR 4 cut(s) 736, 1710, 2440, 2673
Eam1104I CTCTTC 2 cut(s) 897, 1677
EarI CTCTTC 2 cut(s) 897, 1677
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 1 cut(s) 296
Eco24I GRGCYC 1 cut(s) 99
Eco32I GATATC 2 cut(s) 1053, 1764
Eco47I GGWCC 3 cut(s) 1461, 1470, 2279
Eco47III AGCGCT 1 cut(s) 1059
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 5 cut(s) 921, 1251, 1725, 1932, 2664
Eco81I CCTNAGG 2 cut(s) 248, 1983
Eco91I GGTNACC 1 cut(s) 1792
EcoICRI GAGCTC 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 2279
EcoO65I GGTNACC 1 cut(s) 1792
EcoRII CCWGG 2 cut(s) 814, 2505
EcoRV GATATC 2 cut(s) 1053, 1764
EcoT14I CCWWGG 1 cut(s) 296
EcoT22I ATGCAT 2 cut(s) 346, 635
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 296
FalI AAGNNNNNCTT 2 cut(s) 2508, 2540
FaqI GGGAC 1 cut(s) 834
FauI CCCGC 2 cut(s) 217, 2764
FbaI TGATCA 1 cut(s) 856
FriOI GRGCYC 1 cut(s) 99
FspBI CTAG 6 cut(s) 989, 1436, 1842, 1971, 2120, 2267
GlaI GCGC 3 cut(s) 813, 1059, 1932
GsuI CTGGAG 4 cut(s) 220, 450, 818, 1611
HaeII RGCGCY 1 cut(s) 1061
HaeIII GGCC 7 cut(s) 368, 738, 1712, 1964, 2442, 2463, 2675
HapII CCGG 2 cut(s) 711, 2596
HgaI GACGC 3 cut(s) 43, 251, 556
HhaI GCGC 3 cut(s) 814, 1060, 1933
Hin1I GRCGYC 1 cut(s) 243
Hin6I GCGC 3 cut(s) 812, 1058, 1931
HinP1I GCGC 3 cut(s) 812, 1058, 1931
HinfI GANTC 7 cut(s) 184, 222, 731, 940, 1595, 2071, 2664
HpaII CCGG 2 cut(s) 711, 2596
HphI GGTGA 5 cut(s) 58, 76, 866, 1786, 1879
Hpy166II GTNNAC 3 cut(s) 22, 425, 622
Hpy188I TCNGA 6 cut(s) 189, 357, 1768, 1849, 2034, 2468
Hpy188III TCNNGA 8 cut(s) 31, 284, 526, 611, 662, 1590, 1747, 1982
Hpy8I GTNNAC 3 cut(s) 22, 425, 622
Hpy99I CGWCG 6 cut(s) 50, 53, 122, 125, 245, 352
HpyAV CCTTC 8 cut(s) 184, 400, 653, 1793, 1878, 2293, 2534, 2570
HpyCH4III ACNGT 8 cut(s) 619, 772, 871, 1151, 1461, 2148, 2254, 2792
HpyCH4IV ACGT 4 cut(s) 45, 123, 1645, 2028
HpyF10VI GCNNNNNNNGC 6 cut(s) 341, 350, 1124, 1436, 1445, 1454
HpyF3I CTNAG 4 cut(s) 248, 929, 1107, 1983
HpySE526I ACGT 4 cut(s) 45, 123, 1645, 2028
Hsp92I GRCGYC 1 cut(s) 243
HspAI GCGC 3 cut(s) 812, 1058, 1931
Ksp22I TGATCA 1 cut(s) 856
LmnI GCTCC 9 cut(s) 102, 201, 271, 469, 757, 1124, 1561, 2406, 2752
Lsp1109I GCAGC 9 cut(s) 24, 319, 409, 1047, 1367, 1665, 2211, 2464, 2632
LweI GCATC 8 cut(s) 353, 620, 667, 1023, 1182, 1866, 2328, 2367
MaeI CTAG 6 cut(s) 989, 1436, 1842, 1971, 2120, 2267
MaeII ACGT 4 cut(s) 45, 123, 1645, 2028
MfeI CAATTG 2 cut(s) 471, 2104
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 4 cut(s) 24, 99, 466, 896
MlsI TGGCCA 4 cut(s) 738, 1712, 2442, 2675
MluNI TGGCCA 4 cut(s) 738, 1712, 2442, 2675
MlyI GAGTC 2 cut(s) 216, 2658
MmeI TCCRAC 5 cut(s) 735, 1161, 1801, 2077, 2348
Mox20I TGGCCA 4 cut(s) 738, 1712, 2442, 2675
Mph1103I ATGCAT 2 cut(s) 346, 635
MscI TGGCCA 4 cut(s) 738, 1712, 2442, 2675
MslI CAYNNNNRTG 1 cut(s) 2448
Msp20I TGGCCA 4 cut(s) 738, 1712, 2442, 2675
MspA1I CMGCKG 3 cut(s) 212, 328, 962
MspCI CTTAAG 1 cut(s) 1403
MspI CCGG 2 cut(s) 711, 2596
MspR9I CCNGG 3 cut(s) 816, 2507, 2597
MunI CAATTG 2 cut(s) 471, 2104
Mva1269I GAATGC 2 cut(s) 1946, 2109
MvaI CCWGG 2 cut(s) 816, 2507
MwoI GCNNNNNNNGC 6 cut(s) 341, 350, 1124, 1436, 1445, 1454
NciI CCSGG 1 cut(s) 2597
NcoI CCATGG 1 cut(s) 296
NlaIV GGNNCC 3 cut(s) 2281, 2408, 2511
NmeAIII GCCGAG 1 cut(s) 2095
NmuCI GTSAC 5 cut(s) 713, 1045, 1338, 1792, 2683
NsiI ATGCAT 2 cut(s) 346, 635
NspI RCATGY 4 cut(s) 1653, 1701, 1838, 2595
NspV TTCGAA 1 cut(s) 147
OliI CACNNNNGTG 1 cut(s) 2448
PaeI GCATGC 4 cut(s) 1653, 1701, 1838, 2595
PagI TCATGA 3 cut(s) 30, 525, 1746
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 2 cut(s) 1946, 2109
PfeI GAWTC 5 cut(s) 184, 731, 940, 1595, 2071
PflMI CCANNNNNTGG 2 cut(s) 1227, 2570
PleI GAGTC 2 cut(s) 216, 2658
PpsI GAGTC 2 cut(s) 216, 2658
Ppu21I YACGTR 1 cut(s) 1646
PpuMI RGGWCCY 1 cut(s) 2279
PshBI ATTAAT 1 cut(s) 2163
Psp124BI GAGCTC 1 cut(s) 99
Psp5II RGGWCCY 1 cut(s) 2279
Psp6I CCWGG 2 cut(s) 814, 2505
PspEI GGTNACC 1 cut(s) 1792
PspGI CCWGG 2 cut(s) 814, 2505
PspN4I GGNNCC 3 cut(s) 2281, 2408, 2511
PspPI GGNCC 3 cut(s) 1461, 1470, 2279
PspPPI RGGWCCY 1 cut(s) 2279
PstNI CAGNNNCTG 1 cut(s) 2393
PsuI RGATCY 1 cut(s) 607
PvuII CAGCTG 2 cut(s) 328, 962
RsaI GTAC 2 cut(s) 274, 1219
RsaNI GTAC 2 cut(s) 273, 1218
RseI CAYNNNNRTG 1 cut(s) 2448
SacI GAGCTC 1 cut(s) 99
Sau96I GGNCC 3 cut(s) 1461, 1470, 2279
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 2 cut(s) 216, 2658
ScrFI CCNGG 3 cut(s) 816, 2507, 2597
SduI GDGCHC 4 cut(s) 24, 99, 466, 896
SfaNI GCATC 8 cut(s) 353, 620, 667, 1023, 1182, 1866, 2328, 2367
SfcI CTRYAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 147
SinI GGWCC 3 cut(s) 1461, 1470, 2279
SmiMI CAYNNNNRTG 1 cut(s) 2448
SmlI CTYRAG 1 cut(s) 1403
SmoI CTYRAG 1 cut(s) 1403
SphI GCATGC 4 cut(s) 1653, 1701, 1838, 2595
SsiI CCGC 6 cut(s) 210, 984, 1612, 2326, 2719, 2771
SspMI CTAG 6 cut(s) 989, 1436, 1842, 1971, 2120, 2267
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 3 cut(s) 814, 2505, 2595
StyI CCWWGG 1 cut(s) 296
TaaI ACNGT 8 cut(s) 619, 772, 871, 1151, 1461, 2148, 2254, 2792
TaiI ACGT 4 cut(s) 48, 126, 1648, 2031
TaqI TCGA 5 cut(s) 147, 220, 240, 661, 2827
TaqII GACCGA 2 cut(s) 1487, 2621
TatI WGTACW 1 cut(s) 272
TauI GCSGC 2 cut(s) 2329, 2722
TfiI GAWTC 5 cut(s) 184, 731, 940, 1595, 2071
TscAI CASTG 9 cut(s) 29, 801, 901, 1050, 1156, 1929, 2046, 2443, 2450
TseFI GTSAC 5 cut(s) 713, 1045, 1338, 1792, 2683
TseI GCWGC 9 cut(s) 37, 332, 397, 1060, 1380, 1653, 2199, 2477, 2620
Tsp45I GTSAC 5 cut(s) 713, 1045, 1338, 1792, 2683
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 9 cut(s) 29, 801, 901, 1050, 1156, 1929, 2046, 2443, 2450
Van91I CCANNNNNTGG 2 cut(s) 1227, 2570
Vha464I CTTAAG 1 cut(s) 1403
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 3 cut(s) 1461, 1470, 2279
VspI ATTAAT 1 cut(s) 2163
XapI RAATTY 5 cut(s) 965, 1138, 1850, 1900, 2371
XceI RCATGY 4 cut(s) 1653, 1701, 1838, 2595
XspI CTAG 6 cut(s) 989, 1436, 1842, 1971, 2120, 2267
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 2 cut(s) 346, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.