MD16G1131900.v1.1

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009386
Physical Location & Seq
Forward (+)
10057587 .. 10064569
6983 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1131900.v1.1.491

Sequence Viewer

Length: 2892 bp
ATGGGTAAAGGAATATGGATTCACTGGGCGATCATGGCGCTTCACGTCGTCGCCATTCTCTTATTCACCAGAGGCTTTCTTCTCACCCGGACGGAGCTTCCCTTTTACAGCAACTGCTCCGACGCTTCCCAATCGCCTTGCTCATACTCCGTTCCAGAAACCCTGAACCGAAATGACACCGTTGAGCCGAATCAGCAGCGTTGCTGGAGCAAGCCTGCTGTTGGGCGGCTTGTAATCATCGTTTTCGACGCTCTCAGGTTTGATTTTGTAGCTCCGAGTACTTTCTTTCAAGAATTGAAGCCATGGATGAACAAATTACAGCTTGTACAAGACCTTGCAGCTAGAAATGCATCGTCTGCTCGGATTTTCAAAGCCATTGCTGATCCGCCCACGACGAGTTTGCAGCGTTTGAAGGGCTTGACAACTGGTGGATTGCCGACTTTTATTGATGTTGGGAACAGCTTTGGTGCTCCAGCTATAGTTGAAGATAACTTGATACATCAATTGGCTAAAAATGGAAAGCGAGTTGTGATGATGGGGGATGATACATGGACGCAGTTGTTTCCTCATCACTTTGAAAAATCTTTTCCTTACCCCTCTTTTAATGTTAGAGATCTTGATACGGTGGACAGCGGATGCATTGACCACCTTCTTCCATTCTTGTACCAAGAAGATTGGGATGTTCTTATCGCACACTTTCTTGGAGTGGATCATGCTGGGCACATATTTGGGGTTGATTCTGTGCAAATGATAGAAAAGTTGGAGCAATACAACAACGTCTTGCTGAAAGTAGTTGAAGCACTGGAGAGGCAGTCTGCGCCAGGGGGCTTACATGAAAATACTCTGCTACTTGTGATGGGCGATCATGGTCAGACTGTGAATGGAGATCATGGTGGAGGAAGTGCTGAAGAGGTTGAGACATCAATGTTTGCTGTGAGCTTTAAGAATCCTCCATCTCCCATACTATTAGAGTTTGATACTTCATCATGTGAACTAGACTTGGATGGGAGGAATATTTGTGCCAGTTCCTTCCAGCAGCTTGACTTTGCAGTGACAATATCAGCGCTTCTTGGTATCCCCTTTCCTTTTGGAAGTATTGGGCAAGTTAATCCTCAGCTATATGCTTTAGGTGCTGGAACATGGAATTTTGAAGATAGTGTGGGCAATTCCCAAAGTCATTCAAAGTTGGAACAATGGATGCTGAATTATGCCAATATTCTCTGCACCAATTCTTGGCAGGTAAAAAGATATATAGATATTTATTCAGCTTCAGCAGTTATTGGATTTTCCCACGAAGACTTGTTACACATAGCAGGCATGTATGCTAAAGCAGAGGAGAAATGGTCACATGCTACAAAGAAATTGCTTTCACGTGAAAAGGAAGGCCAGAATGAATTATTGCCTGCTCTTAAGAGGCAAATTGATTTGTATTCTGATTTCCTGGCAAGTGTTGCTGAACTTGCACGCTCCAAATGGACTGAGTTTAATTTAAAGATGATGGGTGCTGGACTTGGCATTATGTTGATGTCACTTCTTATGCATTTCATTGCAATTAAGAAAGTGAAAGAGCAATATGGTTTTTCTTTTACTTCATCTGGAGATTCTGGGATATCCTTCGGTTTAGTTTTTTCTTGTTTTATGGTGGTGATGCGTGCATGCAGCTTCCTCTCGAATAGTTTTATCTTGGAAGAAGGAAAAGCGGCATGCTTTCTTTTGGCCACAACTGGACTGATTAAGATGCGATATTCAATCATGAAGAAGAAGATGATATTAGAAGCATTTGTTTTCCTTCTTCTGATCACCATTTGCAGATTTACTATTGAAGTTGGAATGTCAAAGCAGGCTCCTAGTTCTGAAATTATTAATGCATATCCTTCGTGGATGCTCAGGATCACTGCAGGGTTTCCAGTTTGGAATATTCTCGCTGAAGTTTTACCAGTAGTTGCCCTGATTCTATTGGCAATCTTGCTGCGCAAGGCTATTACTGGAAGCTCTTCTGAGGGGATTTGGAAGTATATTGTCATAGGAACCAATATAAGTTATATACTAATAGCAGTGCATTGGGCTTCAGAAAGTAACATATTGAATCTGGATGAGGTGCTTAAAGGCATTGGAAGAAATAACCTTCCTCGACTGATATATGCCATTGGATTTGGACAATTGTTTTTACTGGCATTTAATCAGTTTTTCAATAAAGGGAAATCCTCAGATTGCAGCAAAGTCTTATTTATTAAAACAGTGGCCATGTTTTCTGCCTGGAGCTCAACAGTCATTCTTCTGTTGGGAAAGCAGGGGCCCTGGATTGCTTTAGCATTCCTTATTGGAGGTTATTGCATAATGAGGCTGGATAACATAGAACTGGATGCCAAAGATGGAGGCAGTTGGAATACGTCGCTTGATCCTGTCCCTGTAACTCAATGGAGCCTTTTTGCTGTGTGCCTGTTTTTCTGCACTGGTCATTGGTGTGCTTTTGACGGCCTCCGATACGGTGCTGCATTTATTGGGTTCGATGAGTTCGTCCTAGGTCCCCAAGCAATCCTCCTTACAATGGACACATTTGGTTTTTCTCTCATCCTTCCAATATTTGGACTTCCATTTCTTGTTGCACGCCTGGGTCAGACCGAGAAAGGGAAGAAATTCATGCTCACGCGACTATCTCTAGTGTATATGATTTATGGGGTCATCACGGCCACTTCGGTCACAGCCACTATAATATGCGTTACAATGCATAGGCGGCATCTAATGGTATGGGGTTTATTTGCTCCTAAGTTTGTTTTCGATGTGGCTGGTCTTATCCTCACGGACATACTCGTTTGTGTGGCGTCACATTACTATTTCAGTCGCGTAGAAGATGATGATGATGCTCTACAAGACCCGTTCAGCGAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest

Protein Analysis

964

Amino Acids

107.11

Weight (kDa)

6.02

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphodiest PF01663 217 - 293 7.7e-09 Type I phosphodiesterase / nucleotide pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1973
Acc36I ACCTGC 1 cut(s) 1228
AccB7I CCANNNNNTGG 2 cut(s) 1233, 2585
AccII CGCG 2 cut(s) 2650, 2844
AciI CCGC 5 cut(s) 226, 386, 633, 1700, 2734
AclWI GGATC 4 cut(s) 377, 717, 1898, 2393
AcoI YGGCCR 3 cut(s) 1716, 2241, 2688
AcsI RAATTY 2 cut(s) 1144, 2636
AcuI CTGAAG 4 cut(s) 927, 1254, 1947, 2052
AcvI CACGTG 1 cut(s) 1373
AcyI GRCGYC 1 cut(s) 2822
AfaI GTAC 3 cut(s) 280, 327, 665
AfeI AGCGCT 1 cut(s) 1065
AfiI CCNNNNNNNGG 5 cut(s) 221, 1233, 2548, 2585, 2628
AflII CTTAAG 1 cut(s) 1409
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 5 cut(s) 820, 1440, 2255, 2297, 2610
Alw21I GWGCWC 2 cut(s) 472, 2264
Alw26I GTCTC 1 cut(s) 911
AlwI GGATC 4 cut(s) 377, 717, 1898, 2393
AlwNI CAGNNNCTG 1 cut(s) 114
Aor51HI AGCGCT 1 cut(s) 1065
AoxI GGCC 6 cut(s) 1384, 1716, 2241, 2294, 2476, 2688
ApaI GGGCCC 1 cut(s) 2298
ApeKI GCWGC 8 cut(s) 196, 338, 403, 1036, 1659, 1969, 2214, 2492
ApoI RAATTY 2 cut(s) 1144, 2636
AseI ATTAAT 1 cut(s) 1863
Asp700I GAANNNNTTC 2 cut(s) 1993, 2636
AspA2I CCTAGG 1 cut(s) 2521
AspLEI GCGC 4 cut(s) 40, 820, 1066, 1974
AspS9I GGNCC 3 cut(s) 2294, 2295, 2525
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 4 cut(s) 58, 76, 1657, 1792
AvaII GGWCC 1 cut(s) 2525
AvrII CCTAGG 1 cut(s) 2521
BaeGI GKGCMC 2 cut(s) 723, 2298
BalI TGGCCA 2 cut(s) 1718, 2243
BanII GRGCYC 2 cut(s) 2264, 2298
BarI GAAGNNNNNNTAC 2 cut(s) 1287, 1319
BbrPI CACGTG 1 cut(s) 1373
BbsI GAAGAC 1 cut(s) 1302
Bbv12I GWGCWC 2 cut(s) 472, 2264
BbvCI CCTCAGC 1 cut(s) 1113
BbvI GCAGC 8 cut(s) 208, 350, 415, 1048, 1671, 1956, 2226, 2479
BccI CCATC 6 cut(s) 529, 850, 961, 998, 1492, 2366
BceAI ACGGC 2 cut(s) 2491, 2703
BcgI CGANNNNNNTGC 4 cut(s) 382, 416, 1857, 1891
BciT130I CCWGG 5 cut(s) 822, 1442, 2257, 2299, 2612
BciVI GTATCC 1 cut(s) 1085
BclI TGATCA 1 cut(s) 1797
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 911
BfaI CTAG 5 cut(s) 342, 995, 1848, 2522, 2660
BfmI CTRYAG 2 cut(s) 477, 1896
BfoI RGCGCY 2 cut(s) 41, 1067
BfrI CTTAAG 1 cut(s) 1409
BfuAI ACCTGC 1 cut(s) 1228
BfuI GTATCC 1 cut(s) 1085
BglII AGATCT 1 cut(s) 613
BlnI CCTAGG 1 cut(s) 2521
BmcAI AGTACT 1 cut(s) 280
Bme1390I CCNGG 6 cut(s) 88, 822, 1442, 2257, 2299, 2612
Bme18I GGWCC 1 cut(s) 2525
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 2294, 2295, 2525
BmiI GGNNCC 6 cut(s) 1845, 2029, 2295, 2296, 2423, 2527
BmrFI CCNGG 6 cut(s) 88, 822, 1442, 2257, 2299, 2612
BmrI ACTGGG 1 cut(s) 34
BmsI GCATC 9 cut(s) 359, 626, 1188, 1638, 1728, 1872, 2353, 2746, 2851
BmuI ACTGGG 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 1302
BpmI CTGGAG 5 cut(s) 226, 456, 824, 1617, 2278
Bpu10I CCTNAGC 2 cut(s) 1113, 1886
BpuMI CCSGG 1 cut(s) 88
BsaAI YACGTR 1 cut(s) 1373
BsaHI GRCGYC 1 cut(s) 2822
BsaJI CCNNGG 5 cut(s) 302, 821, 2297, 2521, 2611
BsaXI ACNNNNNCTCC 4 cut(s) 797, 827, 1328, 1358
Bsc4I CCNNNNNNNGG 5 cut(s) 221, 1233, 2548, 2585, 2628
Bse3DI GCAATG 2 cut(s) 375, 1545
BseBI CCWGG 5 cut(s) 822, 1442, 2257, 2299, 2612
BseDI CCNNGG 5 cut(s) 302, 821, 2297, 2521, 2611
BseLI CCNNNNNNNGG 5 cut(s) 221, 1233, 2548, 2585, 2628
BseMI GCAATG 2 cut(s) 375, 1545
BseMII CTCAG 6 cut(s) 268, 1127, 1470, 1900, 1989, 2220
BseRI GAGGAG 1 cut(s) 1349
BseSI GKGCMC 2 cut(s) 723, 2298
BseXI GCAGC 8 cut(s) 208, 350, 415, 1048, 1671, 1956, 2226, 2479
BseYI CCCAGC 1 cut(s) 716
BsgI GTGCAG 2 cut(s) 1207, 2434
Bsh1236I CGCG 2 cut(s) 2650, 2844
BshFI GGCC 6 cut(s) 1386, 1718, 2243, 2296, 2478, 2690
BsiHKAI GWGCWC 2 cut(s) 472, 2264
BsiSI CCGG 1 cut(s) 88
BslFI GGGAC 2 cut(s) 2390, 2511
BslI CCNNNNNNNGG 5 cut(s) 221, 1233, 2548, 2585, 2628
BsmAI GTCTC 1 cut(s) 911
BsmFI GGGAC 2 cut(s) 2390, 2511
BsmI GAATGC 1 cut(s) 2312
BsnI GGCC 6 cut(s) 1386, 1718, 2243, 2296, 2478, 2690
Bsp120I GGGCCC 1 cut(s) 2294
Bsp1286I GDGCHC 4 cut(s) 472, 723, 2264, 2298
Bsp1407I TGTACA 1 cut(s) 325
Bsp143I GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 5 cut(s) 226, 386, 633, 1700, 2734
BspANI GGCC 6 cut(s) 1386, 1718, 2243, 2296, 2478, 2690
BspCNI CTCAG 6 cut(s) 267, 1126, 1471, 1899, 1990, 2219
BspFNI CGCG 2 cut(s) 2650, 2844
BspHI TCATGA 1 cut(s) 1752
BspLI GGNNCC 6 cut(s) 1845, 2029, 2295, 2296, 2423, 2527
BspMAI CTGCAG 1 cut(s) 1900
BspMI ACCTGC 1 cut(s) 1228
BspPI GGATC 4 cut(s) 377, 717, 1898, 2393
BspQI GCTCTTC 1 cut(s) 1999
BspTI CTTAAG 1 cut(s) 1409
BsrDI GCAATG 2 cut(s) 375, 1545
BsrGI TGTACA 1 cut(s) 325
BssECI CCNNGG 5 cut(s) 302, 821, 2297, 2521, 2611
BssMI GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
BssNI GRCGYC 1 cut(s) 2822
BssT1I CCWWGG 2 cut(s) 302, 2521
Bst2UI CCWGG 5 cut(s) 822, 1442, 2257, 2299, 2612
Bst4CI ACNGT 6 cut(s) 181, 625, 877, 2239, 2269, 2489
Bst6I CTCTTC 2 cut(s) 903, 1999
BstACI GRCGYC 1 cut(s) 2822
BstAFI CTTAAG 1 cut(s) 1409
BstAPI GCANNNNNTGC 2 cut(s) 356, 1451
BstAUI TGTACA 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 1373
BstDEI CTNAG 7 cut(s) 254, 1113, 1479, 1886, 1998, 2206, 2766
BstDSI CCRYGG 1 cut(s) 302
BstFNI CGCG 2 cut(s) 2650, 2844
BstH2I RGCGCY 2 cut(s) 41, 1067
BstHHI GCGC 4 cut(s) 40, 820, 1066, 1974
BstKTI GATC 9 cut(s) 33, 385, 616, 712, 865, 889, 1800, 1893, 2401
BstMAI GTCTC 1 cut(s) 911
BstMBI GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
BstMWI GCNNNNNNNGC 9 cut(s) 35, 193, 347, 356, 817, 1130, 1451, 1460, 2734
BstNI CCWGG 5 cut(s) 822, 1442, 2257, 2299, 2612
BstNSI RCATGY 4 cut(s) 1321, 1352, 1659, 1707
BstSCI CCNGG 6 cut(s) 86, 820, 1440, 2255, 2297, 2610
BstSFI CTRYAG 2 cut(s) 477, 1896
BstSLI GKGCMC 2 cut(s) 723, 2298
BstUI CGCG 2 cut(s) 2650, 2844
BstV1I GCAGC 8 cut(s) 208, 350, 415, 1048, 1671, 1956, 2226, 2479
BstV2I GAAGAC 1 cut(s) 1302
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
BsuI GTATCC 1 cut(s) 1085
BsuRI GGCC 6 cut(s) 1386, 1718, 2243, 2296, 2478, 2690
BtgI CCRYGG 1 cut(s) 302
BtrI CACGTC 1 cut(s) 46
BtsI GCAGTG 3 cut(s) 1056, 1893, 2061
BtsIMutI CAGTG 7 cut(s) 22, 800, 1056, 1893, 2061, 2244, 2451
BveI ACCTGC 1 cut(s) 1228
CaiI CAGNNNCTG 1 cut(s) 114
CciI TCATGA 1 cut(s) 1752
CfoI GCGC 4 cut(s) 40, 820, 1066, 1974
Cfr13I GGNCC 3 cut(s) 2294, 2295, 2525
CseI GACGC 4 cut(s) 131, 257, 562, 2811
Csp6I GTAC 3 cut(s) 279, 326, 664
CviQI GTAC 3 cut(s) 279, 326, 664
DdeI CTNAG 7 cut(s) 254, 1113, 1479, 1886, 1998, 2206, 2766
DpnI GATC 9 cut(s) 32, 384, 615, 711, 864, 888, 1799, 1892, 2400
DpnII GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
DraI TTTAAA 1 cut(s) 1491
EaeI YGGCCR 3 cut(s) 1716, 2241, 2688
Eam1104I CTCTTC 2 cut(s) 903, 1999
EarI CTCTTC 2 cut(s) 903, 1999
EciI GGCGGA 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 2262
Eco130I CCWWGG 2 cut(s) 302, 2521
Eco24I GRGCYC 2 cut(s) 2264, 2298
Eco32I GATATC 1 cut(s) 1611
Eco47I GGWCC 1 cut(s) 2525
Eco47III AGCGCT 1 cut(s) 1065
Eco53kI GAGCTC 1 cut(s) 2262
Eco57I CTGAAG 4 cut(s) 927, 1254, 1947, 2052
Eco72I CACGTG 1 cut(s) 1373
EcoICRI GAGCTC 1 cut(s) 2262
EcoO109I RGGNCCY 3 cut(s) 2294, 2295, 2525
EcoRII CCWGG 5 cut(s) 820, 1440, 2255, 2297, 2610
EcoRV GATATC 1 cut(s) 1611
EcoT14I CCWWGG 2 cut(s) 302, 2521
EcoT22I ATGCAT 5 cut(s) 352, 641, 1542, 1870, 2730
EcoT38I GRGCYC 2 cut(s) 2264, 2298
ErhI CCWWGG 2 cut(s) 302, 2521
FaqI GGGAC 2 cut(s) 2390, 2511
FbaI TGATCA 1 cut(s) 1797
FriOI GRGCYC 2 cut(s) 2264, 2298
FspBI CTAG 5 cut(s) 342, 995, 1848, 2522, 2660
FspI TGCGCA 1 cut(s) 1973
GlaI GCGC 4 cut(s) 39, 819, 1065, 1973
GsaI CCCAGC 1 cut(s) 720
GsuI CTGGAG 5 cut(s) 226, 456, 824, 1617, 2278
HaeII RGCGCY 2 cut(s) 41, 1067
HaeIII GGCC 6 cut(s) 1386, 1718, 2243, 2296, 2478, 2690
HapII CCGG 1 cut(s) 88
HgaI GACGC 4 cut(s) 131, 257, 562, 2811
HhaI GCGC 4 cut(s) 40, 820, 1066, 1974
Hin1I GRCGYC 1 cut(s) 2822
Hin6I GCGC 4 cut(s) 38, 818, 1064, 1972
HinP1I GCGC 4 cut(s) 38, 818, 1064, 1972
HinfI GANTC 7 cut(s) 19, 190, 737, 946, 1601, 1951, 2086
HpaII CCGG 1 cut(s) 88
HphI GGTGA 4 cut(s) 58, 76, 1657, 1792
Hpy166II GTNNAC 2 cut(s) 628, 992
Hpy188III TCNNGA 8 cut(s) 155, 290, 617, 1596, 1669, 1753, 1888, 2090
Hpy8I GTNNAC 2 cut(s) 628, 992
Hpy99I CGWCG 6 cut(s) 50, 53, 125, 251, 397, 2395
HpyCH4III ACNGT 6 cut(s) 181, 625, 877, 2239, 2269, 2489
HpyCH4IV ACGT 4 cut(s) 45, 777, 1372, 2390
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 193, 347, 356, 817, 1130, 1451, 1460, 2734
HpyF3I CTNAG 7 cut(s) 254, 1113, 1479, 1886, 1998, 2206, 2766
HpySE526I ACGT 4 cut(s) 45, 777, 1372, 2390
Hsp92I GRCGYC 1 cut(s) 2822
HspAI GCGC 4 cut(s) 38, 818, 1064, 1972
Ksp22I TGATCA 1 cut(s) 1797
Kzo9I GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
LguI GCTCTTC 1 cut(s) 1999
Lsp1109I GCAGC 8 cut(s) 208, 350, 415, 1048, 1671, 1956, 2226, 2479
LweI GCATC 9 cut(s) 359, 626, 1188, 1638, 1728, 1872, 2353, 2746, 2851
MaeI CTAG 5 cut(s) 342, 995, 1848, 2522, 2660
MaeII ACGT 4 cut(s) 45, 777, 1372, 2390
MaeIII GTNAC 9 cut(s) 1051, 1302, 1344, 1527, 2075, 2410, 2698, 2719, 2823
MalI GATC 9 cut(s) 32, 384, 615, 711, 864, 888, 1799, 1892, 2400
MboI GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
MfeI CAATTG 2 cut(s) 503, 2159
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 4 cut(s) 472, 723, 2264, 2298
MlsI TGGCCA 2 cut(s) 1718, 2243
MluNI TGGCCA 2 cut(s) 1718, 2243
MmeI TCCRAC 5 cut(s) 144, 741, 1167, 1807, 2363
Mox20I TGGCCA 2 cut(s) 1718, 2243
Mph1103I ATGCAT 5 cut(s) 352, 641, 1542, 1870, 2730
MroXI GAANNNNTTC 2 cut(s) 1993, 2636
MscI TGGCCA 2 cut(s) 1718, 2243
MslI CAYNNNNRTG 1 cut(s) 2463
Msp20I TGGCCA 2 cut(s) 1718, 2243
MspA1I CMGCKG 1 cut(s) 633
MspCI CTTAAG 1 cut(s) 1409
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 6 cut(s) 88, 822, 1442, 2257, 2299, 2612
MunI CAATTG 2 cut(s) 503, 2159
Mva1269I GAATGC 1 cut(s) 2312
MvaI CCWGG 5 cut(s) 822, 1442, 2257, 2299, 2612
MvnI CGCG 2 cut(s) 2650, 2844
MwoI GCNNNNNNNGC 9 cut(s) 35, 193, 347, 356, 817, 1130, 1451, 1460, 2734
NciI CCSGG 1 cut(s) 88
NcoI CCATGG 1 cut(s) 302
NdeII GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
NlaIV GGNNCC 6 cut(s) 1845, 2029, 2295, 2296, 2423, 2527
NmuCI GTSAC 5 cut(s) 1051, 1344, 1527, 2698, 2823
NsbI TGCGCA 1 cut(s) 1973
NsiI ATGCAT 5 cut(s) 352, 641, 1542, 1870, 2730
NspI RCATGY 4 cut(s) 1321, 1352, 1659, 1707
PaeI GCATGC 2 cut(s) 1659, 1707
PagI TCATGA 1 cut(s) 1752
PciSI GCTCTTC 1 cut(s) 1999
PcsI WCGNNNNNNNCGW 1 cut(s) 2514
PctI GAATGC 1 cut(s) 2312
PdmI GAANNNNTTC 2 cut(s) 1993, 2636
PfeI GAWTC 7 cut(s) 19, 190, 737, 946, 1601, 1951, 2086
PflMI CCANNNNNTGG 2 cut(s) 1233, 2585
PmaCI CACGTG 1 cut(s) 1373
PmlI CACGTG 1 cut(s) 1373
Ppu21I YACGTR 1 cut(s) 1373
PpuMI RGGWCCY 1 cut(s) 2525
PshBI ATTAAT 1 cut(s) 1863
Psp124BI GAGCTC 1 cut(s) 2264
Psp5II RGGWCCY 1 cut(s) 2525
Psp6I CCWGG 5 cut(s) 820, 1440, 2255, 2297, 2610
PspCI CACGTG 1 cut(s) 1373
PspFI CCCAGC 1 cut(s) 716
PspGI CCWGG 5 cut(s) 820, 1440, 2255, 2297, 2610
PspN4I GGNNCC 6 cut(s) 1845, 2029, 2295, 2296, 2423, 2527
PspOMI GGGCCC 1 cut(s) 2294
PspPI GGNCC 3 cut(s) 2294, 2295, 2525
PspPPI RGGWCCY 1 cut(s) 2525
PstI CTGCAG 1 cut(s) 1900
PstNI CAGNNNCTG 1 cut(s) 114
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 3 cut(s) 280, 327, 665
RsaNI GTAC 3 cut(s) 279, 326, 664
RseI CAYNNNNRTG 1 cut(s) 2463
SacI GAGCTC 1 cut(s) 2264
SapI GCTCTTC 1 cut(s) 1999
Sau3AI GATC 9 cut(s) 30, 382, 613, 709, 862, 886, 1797, 1890, 2398
Sau96I GGNCC 3 cut(s) 2294, 2295, 2525
ScaI AGTACT 1 cut(s) 280
ScrFI CCNGG 6 cut(s) 88, 822, 1442, 2257, 2299, 2612
SduI GDGCHC 4 cut(s) 472, 723, 2264, 2298
SfaNI GCATC 9 cut(s) 359, 626, 1188, 1638, 1728, 1872, 2353, 2746, 2851
SfcI CTRYAG 2 cut(s) 477, 1896
SinI GGWCC 1 cut(s) 2525
SmiMI CAYNNNNRTG 1 cut(s) 2463
SmlI CTYRAG 1 cut(s) 1409
SmoI CTYRAG 1 cut(s) 1409
SphI GCATGC 2 cut(s) 1659, 1707
SsiI CCGC 5 cut(s) 226, 386, 633, 1700, 2734
SspI AATATT 4 cut(s) 1015, 1216, 1918, 2583
SspMI CTAG 5 cut(s) 342, 995, 1848, 2522, 2660
SstI GAGCTC 1 cut(s) 2264
StyD4I CCNGG 6 cut(s) 86, 820, 1440, 2255, 2297, 2610
StyI CCWWGG 2 cut(s) 302, 2521
TaaI ACNGT 6 cut(s) 181, 625, 877, 2239, 2269, 2489
TaiI ACGT 4 cut(s) 48, 780, 1375, 2393
TaqI TCGA 5 cut(s) 246, 1670, 2131, 2508, 2778
TaqII GACCGA 2 cut(s) 2636, 2686
TatI WGTACW 2 cut(s) 278, 325
TauI GCSGC 3 cut(s) 229, 1703, 2737
TfiI GAWTC 7 cut(s) 19, 190, 737, 946, 1601, 1951, 2086
TscAI CASTG 7 cut(s) 29, 807, 1056, 1900, 2061, 2244, 2458
TseFI GTSAC 5 cut(s) 1051, 1344, 1527, 2698, 2823
TseI GCWGC 8 cut(s) 196, 338, 403, 1036, 1659, 1969, 2214, 2492
Tsp45I GTSAC 5 cut(s) 1051, 1344, 1527, 2698, 2823
TspDTI ATGAA 8 cut(s) 323, 849, 972, 1407, 1534, 1581, 1769, 2629
TspGWI ACGGA 3 cut(s) 107, 139, 2816
TspRI CASTG 7 cut(s) 29, 807, 1056, 1900, 2061, 2244, 2458
Van91I CCANNNNNTGG 2 cut(s) 1233, 2585
Vha464I CTTAAG 1 cut(s) 1409
VpaK11BI GGWCC 1 cut(s) 2525
VspI ATTAAT 1 cut(s) 1863
XapI RAATTY 2 cut(s) 1144, 2636
XceI RCATGY 4 cut(s) 1321, 1352, 1659, 1707
XmaJI CCTAGG 1 cut(s) 2521
XmnI GAANNNNTTC 2 cut(s) 1993, 2636
XspI CTAG 5 cut(s) 342, 995, 1848, 2522, 2660
ZrmI AGTACT 1 cut(s) 280
Zsp2I ATGCAT 5 cut(s) 352, 641, 1542, 1870, 2730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.