MD13G1171700.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010256
Physical Location & Seq
Reverse (-)
14009707 .. 14013327
3621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1171700.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGACTCGTTGTTAAGAGAACCGACGAGGATAAGCAGCCGGGAGAAGCGGCTGCGGAGGGTAATATTGCCGGCAATCCGAGGGCAAAACTGTCCAATATGCTTGGTTGCCGTGGAAGCTCGCAGCGCAGCGGTGCTCTCGTCCTGTAACCACGCGTACTGCGTCCGTTGCATCCGGAAGTGGAGCAATTTGAGACGAAAATGCCCTCTCTGCAACGCCGACTTCGACTCCTGGTTCTCGCAAATCAGCCTCTCTTCCCGAAGCTTCCACAAAGAACTTTTACCTCCTCTGAATCGCACTTCTAGTGCTAGAAGCTTCGGCCTTCAACACGAAGAGCATCCTCCAAGGCATGTGAGAAGTAGTGAACGGCGGCGATCAAGGCCGTTGCCGTGGCGGCGATCGTTTGGACGGCCAGGATCGGTCGGTCCTGATACGGTTGCGGAGAGAAAGCTCCAATGGCGAGCTAGTGTATATGACAGACGATTGCAAGCTGTTCCTTCAGCTTCAAGAAATCGTCTTCAAGTGAGTGTACCCATAACCGATGGTGTAAAAGAGAGAATTTTGCAAAGAATAGAGCCATGGATTCGGAGGGAGTTGCAGGCTCTTCTTGGAGACGGAGATCCATCTATTATTGTTCATGTGGCCACCTCACTCTTCATTGCAAACCTTGAAAATAAGGATCAGGTCCTTCCAGGACAGTGTGATGTCCGAGGTGACCCCCTTGCATCGTTGCGCCCTTTTCTAGTTGATCGAACTGACATGTTTTTGCACGAGTTAAGATGTTTTGCAGAGAGTGCATTCAACATGGAAACCTATGACGCAGTGGTTGAGTATAAAAGCTTGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0000922 GO:0000930 GO:0001654 GO:0001754 GO:0003407 GO:0003674 GO:0003676 GO:0003677 GO:0003823 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005814 GO:0005815 GO:0005819 GO:0005856 GO:0005929 GO:0006139 GO:0006351 GO:0006464 GO:0006508 GO:0006511 GO:0006513 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009653 GO:0009887 GO:0009893 GO:0009987 GO:0010467 GO:0010498 GO:0010604 GO:0010842 GO:0010941 GO:0012501 GO:0015630 GO:0016043 GO:0016070 GO:0016567 GO:0016604 GO:0016605 GO:0016607 GO:0016740 GO:0016925 GO:0018130 GO:0018193 GO:0018205 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019789 GO:0019899 GO:0019941 GO:0022008 GO:0023052 GO:0030154 GO:0030163 GO:0030182 GO:0030496 GO:0031323 GO:0031325 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032391 GO:0032446 GO:0032501 GO:0032502 GO:0032507 GO:0032774 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035556 GO:0035845 GO:0035869 GO:0036064 GO:0036211 GO:0042461 GO:0042462 GO:0042670 GO:0042981 GO:0042995 GO:0043005 GO:0043010 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044451 GO:0044463 GO:0044464 GO:0044547 GO:0045185 GO:0046483 GO:0046530 GO:0046548 GO:0046549 GO:0048468 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048592 GO:0048593 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051235 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051438 GO:0051443 GO:0051457 GO:0051603 GO:0051641 GO:0051651 GO:0051716 GO:0060041 GO:0060042 GO:0060219 GO:0060255 GO:0060548 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070647 GO:0070727 GO:0070936 GO:0071704 GO:0071840 GO:0072595 GO:0080090 GO:0090304 GO:0090596 GO:0097159 GO:0097190 GO:0097193 GO:0097458 GO:0097659 GO:0097730 GO:0097731 GO:0097733 GO:0120025 GO:0120038 GO:0140096 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1902494 GO:1903320 GO:1903322 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWI zf-C3HC4 zf-C3HC4_3 zf-RING_2

Protein Analysis

283

Amino Acids

32.39

Weight (kDa)

10.15

Isoelectric Point (pI)

73.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 29 - 72 2.6e-08 RING-H2 zinc finger domain
zf-RING_2 PF13639 30 - 72 3.2e-09 Ring finger domain
zf-RING_5 PF14634 30 - 72 1.9e-06 zinc-RING finger domain
zf-C3HC4 PF00097 31 - 71 4.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 31 - 71 7.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 155
AccIII TCCGGA 1 cut(s) 174
AciI CCGC 6 cut(s) 49, 55, 131, 370, 394, 440
AclWI GGATC 3 cut(s) 424, 614, 687
AcoI YGGCCR 2 cut(s) 410, 642
AcsI RAATTY 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 483
AfaI GTAC 2 cut(s) 158, 531
AflIII ACRYGT 2 cut(s) 153, 759
AgsI TTSAA 5 cut(s) 326, 507, 521, 671, 802
AjnI CCWGG 3 cut(s) 230, 412, 691
AluBI AGCT 8 cut(s) 119, 264, 315, 451, 464, 491, 503, 840
AluI AGCT 8 cut(s) 119, 264, 315, 451, 464, 491, 503, 840
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 2 cut(s) 187, 606
AlwI GGATC 3 cut(s) 424, 614, 687
Aor13HI TCCGGA 1 cut(s) 174
AoxI GGCC 4 cut(s) 319, 380, 410, 642
ApeKI GCWGC 4 cut(s) 36, 52, 123, 128
ApoI RAATTY 1 cut(s) 558
AspLEI GCGC 2 cut(s) 128, 735
AspS9I GGNCC 2 cut(s) 425, 685
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 725
AvaII GGWCC 2 cut(s) 425, 685
BalI TGGCCA 1 cut(s) 644
BauI CACGAG 1 cut(s) 770
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 4 cut(s) 39, 48, 135, 140
BccI CCATC 2 cut(s) 536, 631
BceAI ACGGC 5 cut(s) 95, 367, 373, 383, 425
BciT130I CCWGG 3 cut(s) 232, 414, 693
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 2 cut(s) 187, 606
BfaI CTAG 4 cut(s) 303, 309, 465, 743
BglI GCCNNNNNGGC 1 cut(s) 394
BisI GCNGC 7 cut(s) 37, 50, 53, 124, 129, 371, 395
BlsI GCNGC 7 cut(s) 38, 51, 54, 125, 130, 372, 396
Bme1390I CCNGG 4 cut(s) 41, 232, 414, 693
Bme18I GGWCC 2 cut(s) 425, 685
BmgT120I GGNCC 2 cut(s) 425, 685
BmrFI CCNGG 4 cut(s) 41, 232, 414, 693
BmsI GCATC 3 cut(s) 180, 346, 734
BpiI GAAGAC 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 41
BsaJI CCNNGG 6 cut(s) 79, 111, 344, 389, 578, 709
BsaWI WCCGGW 1 cut(s) 174
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bse118I RCCGGY 1 cut(s) 70
Bse3DI GCAATG 1 cut(s) 657
BseAI TCCGGA 1 cut(s) 174
BseBI CCWGG 3 cut(s) 232, 414, 693
BseDI CCNNGG 6 cut(s) 79, 111, 344, 389, 578, 709
BseGI GGATG 2 cut(s) 171, 337
BseMI GCAATG 1 cut(s) 657
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 4 cut(s) 39, 48, 135, 140
Bsh1236I CGCG 1 cut(s) 155
Bsh1285I CGRYCG 2 cut(s) 401, 423
BshFI GGCC 4 cut(s) 321, 382, 412, 644
BsiEI CGRYCG 2 cut(s) 401, 423
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 3 cut(s) 40, 71, 175
BsmAI GTCTC 2 cut(s) 187, 606
BsmBI CGTCTC 2 cut(s) 187, 606
BsmI GAATGC 1 cut(s) 797
BsnI GGCC 4 cut(s) 321, 382, 412, 644
Bsp1286I GDGCHC 1 cut(s) 138
Bsp13I TCCGGA 1 cut(s) 174
Bsp143I GATC 6 cut(s) 374, 398, 416, 619, 679, 748
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 6 cut(s) 49, 55, 131, 370, 394, 440
BspANI GGCC 4 cut(s) 321, 382, 412, 644
BspEI TCCGGA 1 cut(s) 174
BspFNI CGCG 1 cut(s) 155
BspPI GGATC 3 cut(s) 424, 614, 687
BspQI GCTCTTC 2 cut(s) 327, 609
BsrDI GCAATG 1 cut(s) 657
BsrFI RCCGGY 1 cut(s) 70
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 6 cut(s) 79, 111, 344, 389, 578, 709
BssMI GATC 6 cut(s) 374, 398, 416, 619, 679, 748
BssSI CACGAG 1 cut(s) 770
BssT1I CCWWGG 2 cut(s) 344, 578
Bst2BI CACGAG 1 cut(s) 770
Bst2UI CCWGG 3 cut(s) 232, 414, 693
Bst4CI ACNGT 3 cut(s) 92, 436, 699
Bst6I CTCTTC 4 cut(s) 259, 327, 609, 659
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 6 cut(s) 72, 121, 462, 489, 600, 842
BstDSI CCRYGG 3 cut(s) 111, 389, 578
BstEII GGTNACC 1 cut(s) 713
BstF5I GGATG 2 cut(s) 171, 337
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 2 cut(s) 128, 735
BstKTI GATC 6 cut(s) 377, 401, 419, 622, 682, 751
BstMAI GTCTC 2 cut(s) 187, 606
BstMBI GATC 6 cut(s) 374, 398, 416, 619, 679, 748
BstMCI CGRYCG 2 cut(s) 401, 423
BstMWI GCNNNNNNNGC 8 cut(s) 116, 125, 168, 210, 379, 394, 457, 794
BstNI CCWGG 3 cut(s) 232, 414, 693
BstNSI RCATGY 2 cut(s) 353, 763
BstPI GGTNACC 1 cut(s) 713
BstSCI CCNGG 4 cut(s) 39, 230, 412, 691
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 4 cut(s) 39, 48, 135, 140
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
BsuRI GGCC 4 cut(s) 321, 382, 412, 644
BtgI CCRYGG 3 cut(s) 111, 389, 578
BtsCI GGATG 2 cut(s) 171, 337
BtsI GCAGTG 1 cut(s) 828
BtsIMutI CAGTG 2 cut(s) 704, 828
Cac8I GCNNGC 6 cut(s) 72, 121, 462, 489, 600, 842
CfoI GCGC 2 cut(s) 128, 735
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 2 cut(s) 425, 685
CseI GACGC 2 cut(s) 151, 827
Csp6I GTAC 2 cut(s) 157, 530
CviAII CATG 5 cut(s) 350, 579, 638, 760, 805
CviQI GTAC 2 cut(s) 157, 530
DpnI GATC 6 cut(s) 376, 400, 418, 621, 681, 750
DpnII GATC 6 cut(s) 374, 398, 416, 619, 679, 748
EaeI YGGCCR 2 cut(s) 410, 642
Eam1104I CTCTTC 4 cut(s) 259, 327, 609, 659
EarI CTCTTC 4 cut(s) 259, 327, 609, 659
Eco130I CCWWGG 2 cut(s) 344, 578
Eco47I GGWCC 2 cut(s) 425, 685
Eco57I CTGAAG 1 cut(s) 483
Eco91I GGTNACC 1 cut(s) 713
EcoO109I RGGNCCY 1 cut(s) 685
EcoO65I GGTNACC 1 cut(s) 713
EcoRII CCWGG 3 cut(s) 230, 412, 691
EcoT14I CCWWGG 2 cut(s) 344, 578
ErhI CCWWGG 2 cut(s) 344, 578
Esp3I CGTCTC 2 cut(s) 187, 606
FaeI CATG 5 cut(s) 353, 582, 641, 763, 808
FatI CATG 5 cut(s) 349, 578, 637, 759, 804
Fnu4HI GCNGC 7 cut(s) 37, 50, 53, 124, 129, 371, 395
FokI GGATG 2 cut(s) 158, 324
Fsp4HI GCNGC 7 cut(s) 37, 50, 53, 124, 129, 371, 395
FspBI CTAG 4 cut(s) 303, 309, 465, 743
GlaI GCGC 2 cut(s) 127, 734
GluI GCNGC 7 cut(s) 37, 50, 53, 124, 129, 371, 395
HaeIII GGCC 4 cut(s) 321, 382, 412, 644
HapII CCGG 3 cut(s) 40, 71, 175
HgaI GACGC 2 cut(s) 151, 827
HhaI GCGC 2 cut(s) 128, 735
Hin1II CATG 5 cut(s) 353, 582, 641, 763, 808
Hin6I GCGC 2 cut(s) 126, 733
HinP1I GCGC 2 cut(s) 126, 733
HindIII AAGCTT 3 cut(s) 262, 313, 838
HinfI GANTC 4 cut(s) 5, 227, 292, 583
HpaII CCGG 3 cut(s) 40, 71, 175
HphI GGTGA 1 cut(s) 725
Hpy166II GTNNAC 2 cut(s) 365, 530
Hpy188I TCNGA 4 cut(s) 80, 291, 588, 710
Hpy188III TCNNGA 4 cut(s) 175, 258, 428, 507
Hpy8I GTNNAC 2 cut(s) 365, 530
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 3 cut(s) 332, 507, 698
HpyCH4III ACNGT 3 cut(s) 92, 436, 699
HpyF10VI GCNNNNNNNGC 8 cut(s) 116, 125, 168, 210, 379, 394, 457, 794
Hsp92II CATG 5 cut(s) 353, 582, 641, 763, 808
HspAI GCGC 2 cut(s) 126, 733
Kpn2I TCCGGA 1 cut(s) 174
KroI GCCGGC 1 cut(s) 70
KroNI GCCGGC 1 cut(s) 72
Kzo9I GATC 6 cut(s) 374, 398, 416, 619, 679, 748
LguI GCTCTTC 2 cut(s) 327, 609
LmnI GCTCC 2 cut(s) 183, 456
Lsp1109I GCAGC 4 cut(s) 39, 48, 135, 140
LweI GCATC 3 cut(s) 180, 346, 734
MaeI CTAG 4 cut(s) 303, 309, 465, 743
MaeIII GTNAC 2 cut(s) 146, 713
MalI GATC 6 cut(s) 376, 400, 418, 621, 681, 750
MboI GATC 6 cut(s) 374, 398, 416, 619, 679, 748
MboII GAAGA 5 cut(s) 246, 344, 509, 596, 646
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 644
MluCI AATT 2 cut(s) 187, 558
MluI ACGCGT 1 cut(s) 153
MluNI TGGCCA 1 cut(s) 644
MlyI GAGTC 1 cut(s) 221
Mox20I TGGCCA 1 cut(s) 644
MroI TCCGGA 1 cut(s) 174
MroNI GCCGGC 1 cut(s) 70
MscI TGGCCA 1 cut(s) 644
MseI TTAA 2 cut(s) 14, 776
Msp20I TGGCCA 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 131
MspI CCGG 3 cut(s) 40, 71, 175
MspR9I CCNGG 4 cut(s) 41, 232, 414, 693
Mva1269I GAATGC 1 cut(s) 797
MvaI CCWGG 3 cut(s) 232, 414, 693
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 8 cut(s) 116, 125, 168, 210, 379, 394, 457, 794
NaeI GCCGGC 1 cut(s) 72
NciI CCSGG 1 cut(s) 41
NcoI CCATGG 1 cut(s) 578
NdeII GATC 6 cut(s) 374, 398, 416, 619, 679, 748
NgoMIV GCCGGC 1 cut(s) 70
NlaIII CATG 5 cut(s) 353, 582, 641, 763, 808
NmuCI GTSAC 1 cut(s) 713
NspI RCATGY 2 cut(s) 353, 763
PciI ACATGT 1 cut(s) 759
PciSI GCTCTTC 2 cut(s) 327, 609
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 222
PctI GAATGC 1 cut(s) 797
PdiI GCCGGC 1 cut(s) 72
PfeI GAWTC 2 cut(s) 292, 583
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 7 cut(s) 38, 51, 54, 125, 130, 372, 396
Ple19I CGATCG 1 cut(s) 401
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 685
PscI ACATGT 1 cut(s) 759
Psp5II RGGWCCY 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 230, 412, 691
PspEI GGTNACC 1 cut(s) 713
PspGI CCWGG 3 cut(s) 230, 412, 691
PspPI GGNCC 2 cut(s) 425, 685
PspPPI RGGWCCY 1 cut(s) 685
PsuI RGATCY 1 cut(s) 619
PvuI CGATCG 1 cut(s) 401
RsaI GTAC 2 cut(s) 158, 531
RsaNI GTAC 2 cut(s) 157, 530
SapI GCTCTTC 2 cut(s) 327, 609
SaqAI TTAA 2 cut(s) 14, 776
SatI GCNGC 7 cut(s) 37, 50, 53, 124, 129, 371, 395
Sau3AI GATC 6 cut(s) 374, 398, 416, 619, 679, 748
Sau96I GGNCC 2 cut(s) 425, 685
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 4 cut(s) 41, 232, 414, 693
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 3 cut(s) 180, 346, 734
SinI GGWCC 2 cut(s) 425, 685
Sse9I AATT 2 cut(s) 187, 558
SsiI CCGC 6 cut(s) 49, 55, 131, 370, 394, 440
SspI AATATT 1 cut(s) 66
SspMI CTAG 4 cut(s) 303, 309, 465, 743
StyD4I CCNGG 4 cut(s) 39, 230, 412, 691
StyI CCWWGG 2 cut(s) 344, 578
TaaI ACNGT 3 cut(s) 92, 436, 699
TaqI TCGA 2 cut(s) 225, 751
TaqII GACCGA 2 cut(s) 409, 413
TasI AATT 2 cut(s) 187, 558
TauI GCSGC 3 cut(s) 52, 373, 397
TfiI GAWTC 2 cut(s) 292, 583
Tru1I TTAA 2 cut(s) 14, 776
Tru9I TTAA 2 cut(s) 14, 776
TscAI CASTG 2 cut(s) 704, 828
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 4 cut(s) 36, 52, 123, 128
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 2 cut(s) 626, 646
TspGWI ACGGA 2 cut(s) 155, 630
TspRI CASTG 2 cut(s) 704, 828
VpaK11BI GGWCC 2 cut(s) 425, 685
XapI RAATTY 1 cut(s) 558
XceI RCATGY 2 cut(s) 353, 763
XspI CTAG 4 cut(s) 303, 309, 465, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.