Prupe.1G140100_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010256
Physical Location & Seq
Reverse (-)
10996241 .. 10998989
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G140100.2

Sequence Viewer

Length: 861 bp
ATGGAAGCGTCTCCAAGAAAACCATCGGAGAGAAGCAGGAGCCGCGAGAAGCTGGTGGGGAGGGTAATATGGCCGGCAACCCGAGGGCAAAATTGTCCAATATGCTTGGGAGCCCTGGAACCTCGCAGCGCAGCGGTGCTCACGTCGTGTAACCACGCCTACTGCGTCCGATGCATTCGAAAATGGAGCGGGTTGAGACGAAAATGCCCTCTCTGCAACGCCGACTTCGATTCTTGGTTCTCCTATATCAGCCTATCTTCTCGAAGCTTCCACAGAGAGATTTTGCCACCTCTGGATAACACTTCTAGTTCTAGAAGCTTCAGACTTCAACAAGAAGAGCATCCTTCAAGGCATGTGAGAAATAGCGAACGGAGGCGATCAAGGCCTTTGCCATGGCGGCGATCGTTTGGACGGCCAGGATCAGTCACTCCTGATGTGATTGCCGAAAGGAAGCTTCAATGGCGTGTCAGCAGTGTATATGAAAGACGCTTGCAAGCTGTTCCTTCAGCTCCAAGAAGTCGTCTTCAAGTGAGTGTACCAATAAATGATGGTGTTAAAGAGAGAATTTTGCGAAGAATAGAACCATGGATTCGTAGGGAGCTGCAGGCTCTTCTCGGAGACCGAGATCCATCTATTATTGTTCATGTGGCCACCTCACTCTTCATTGCAAGCCTCGAAAACGAGGGTCATGTCCCTTCAGGACAGTGTGATGTTCGAGATGACTTCCTTGCACGGTTGCGCCCTTTTCTGCTTGATCGAACTGATATGTTTTGGCACGAGCTAAGATGTTTTGCCAAGAGTTCATTTAACATGGAAACCTATGATGCAGTGGTTGAGTATAAAAGGCTCCGTCAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0000922 GO:0000930 GO:0001654 GO:0001754 GO:0003407 GO:0003674 GO:0003676 GO:0003677 GO:0003823 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005814 GO:0005815 GO:0005819 GO:0005856 GO:0005929 GO:0006139 GO:0006351 GO:0006464 GO:0006508 GO:0006511 GO:0006513 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009653 GO:0009887 GO:0009893 GO:0009987 GO:0010467 GO:0010498 GO:0010604 GO:0010842 GO:0010941 GO:0012501 GO:0015630 GO:0016043 GO:0016070 GO:0016567 GO:0016604 GO:0016605 GO:0016607 GO:0016740 GO:0016925 GO:0018130 GO:0018193 GO:0018205 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019789 GO:0019899 GO:0019941 GO:0022008 GO:0023052 GO:0030154 GO:0030163 GO:0030182 GO:0030496 GO:0031323 GO:0031325 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032391 GO:0032446 GO:0032501 GO:0032502 GO:0032507 GO:0032774 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035556 GO:0035845 GO:0035869 GO:0036064 GO:0036211 GO:0042461 GO:0042462 GO:0042670 GO:0042981 GO:0042995 GO:0043005 GO:0043010 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044451 GO:0044463 GO:0044464 GO:0044547 GO:0045185 GO:0046483 GO:0046530 GO:0046548 GO:0046549 GO:0048468 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048592 GO:0048593 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051235 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051438 GO:0051443 GO:0051457 GO:0051603 GO:0051641 GO:0051651 GO:0051716 GO:0060041 GO:0060042 GO:0060219 GO:0060255 GO:0060548 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070647 GO:0070727 GO:0070936 GO:0071704 GO:0071840 GO:0072595 GO:0080090 GO:0090304 GO:0090596 GO:0097159 GO:0097190 GO:0097193 GO:0097458 GO:0097659 GO:0097730 GO:0097731 GO:0097733 GO:0120025 GO:0120038 GO:0140096 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1902494 GO:1903320 GO:1903322 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWI zf-C3HC4 zf-C3HC4_3 zf-RING_2

Protein Analysis

287

Amino Acids

33.32

Weight (kDa)

10.4

Isoelectric Point (pI)

77.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 189
AccII CGCG 1 cut(s) 45
AciI CCGC 4 cut(s) 43, 134, 189, 397
AclWI GGATC 2 cut(s) 427, 620
AcoI YGGCCR 3 cut(s) 71, 413, 648
AcsI RAATTY 1 cut(s) 564
AcuI CTGAAG 3 cut(s) 304, 489, 681
AdeI CACNNNGTG 1 cut(s) 147
AfaI GTAC 1 cut(s) 537
AgsI TTSAA 4 cut(s) 329, 348, 458, 527
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 2 cut(s) 114, 415
AluBI AGCT 8 cut(s) 52, 267, 318, 454, 497, 509, 601, 781
AluI AGCT 8 cut(s) 52, 267, 318, 454, 497, 509, 601, 781
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 3 cut(s) 15, 190, 612
AlwI GGATC 2 cut(s) 427, 620
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 4 cut(s) 71, 383, 413, 648
ApeKI GCWGC 3 cut(s) 126, 131, 601
ApoI RAATTY 1 cut(s) 564
AspLEI GCGC 2 cut(s) 131, 741
AsuII TTCGAA 1 cut(s) 178
AvaI CYCGRG 1 cut(s) 81
BalI TGGCCA 1 cut(s) 650
BanII GRGCYC 1 cut(s) 115
BauI CACGAG 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 138, 143, 588
BccI CCATC 3 cut(s) 31, 542, 637
BceAI ACGGC 1 cut(s) 428
BciT130I CCWGG 2 cut(s) 116, 417
BcoDI GTCTC 3 cut(s) 15, 190, 612
BfaI CTAG 2 cut(s) 306, 312
BfmI CTRYAG 1 cut(s) 602
BglI GCCNNNNNGGC 1 cut(s) 397
BisI GCNGC 5 cut(s) 43, 127, 132, 398, 602
BlsI GCNGC 5 cut(s) 44, 128, 133, 399, 603
Bme1390I CCNGG 2 cut(s) 116, 417
BmeT110I CYCGRG 1 cut(s) 81
BmgBI CACGTC 1 cut(s) 144
BmiI GGNNCC 4 cut(s) 41, 112, 120, 848
BmrFI CCNGG 2 cut(s) 116, 417
BmsI GCATC 3 cut(s) 161, 349, 814
BpiI GAAGAC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 178
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 4 cut(s) 82, 114, 392, 584
Bse118I RCCGGY 1 cut(s) 73
Bse3DI GCAATG 1 cut(s) 663
BseBI CCWGG 2 cut(s) 116, 417
BseDI CCNNGG 4 cut(s) 82, 114, 392, 584
BseGI GGATG 1 cut(s) 340
BseMI GCAATG 1 cut(s) 663
BseXI GCAGC 3 cut(s) 138, 143, 588
Bsh1236I CGCG 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 404
BshFI GGCC 4 cut(s) 73, 385, 415, 650
BsiEI CGRYCG 1 cut(s) 404
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 677
BsmAI GTCTC 3 cut(s) 15, 190, 612
BsmBI CGTCTC 2 cut(s) 15, 190
BsmFI GGGAC 1 cut(s) 677
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 4 cut(s) 73, 385, 415, 650
Bso31I GGTCTC 1 cut(s) 612
BsoBI CYCGRG 1 cut(s) 81
Bsp119I TTCGAA 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 115, 141
Bsp143I GATC 5 cut(s) 377, 401, 419, 625, 754
Bsp19I CCATGG 2 cut(s) 392, 584
BspACI CCGC 4 cut(s) 43, 134, 189, 397
BspANI GGCC 4 cut(s) 73, 385, 415, 650
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 4 cut(s) 41, 112, 120, 848
BspMAI CTGCAG 1 cut(s) 606
BspPI GGATC 2 cut(s) 427, 620
BspQI GCTCTTC 2 cut(s) 330, 615
BspT104I TTCGAA 1 cut(s) 178
BspTNI GGTCTC 1 cut(s) 612
BsrBI CCGCTC 1 cut(s) 189
BsrDI GCAATG 1 cut(s) 663
BsrFI RCCGGY 1 cut(s) 73
BssAI RCCGGY 1 cut(s) 73
BssECI CCNNGG 4 cut(s) 82, 114, 392, 584
BssMI GATC 5 cut(s) 377, 401, 419, 625, 754
BssSI CACGAG 1 cut(s) 776
BssT1I CCWWGG 2 cut(s) 392, 584
Bst2BI CACGAG 1 cut(s) 776
Bst2UI CCWGG 2 cut(s) 116, 417
Bst4CI ACNGT 2 cut(s) 705, 735
Bst6I CTCTTC 3 cut(s) 330, 615, 665
BstBI TTCGAA 1 cut(s) 178
BstC8I GCNNGC 5 cut(s) 75, 491, 495, 606, 670
BstDEI CTNAG 1 cut(s) 782
BstDSI CCRYGG 2 cut(s) 392, 584
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 2 cut(s) 131, 741
BstKTI GATC 5 cut(s) 380, 404, 422, 628, 757
BstMAI GTCTC 3 cut(s) 15, 190, 612
BstMBI GATC 5 cut(s) 377, 401, 419, 625, 754
BstMCI CGRYCG 1 cut(s) 404
BstMWI GCNNNNNNNGC 6 cut(s) 42, 171, 213, 382, 397, 460
BstNI CCWGG 2 cut(s) 116, 417
BstNSI RCATGY 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 114, 415
BstSFI CTRYAG 1 cut(s) 602
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 3 cut(s) 138, 143, 588
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 4 cut(s) 73, 385, 415, 650
BtgI CCRYGG 2 cut(s) 392, 584
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 1 cut(s) 340
BtsI GCAGTG 2 cut(s) 478, 834
BtsIMutI CAGTG 3 cut(s) 478, 710, 834
Cac8I GCNNGC 5 cut(s) 75, 491, 495, 606, 670
CfoI GCGC 2 cut(s) 131, 741
Cfr10I RCCGGY 1 cut(s) 73
CseI GACGC 2 cut(s) 154, 495
Csp6I GTAC 1 cut(s) 536
CviAII CATG 6 cut(s) 353, 393, 585, 644, 689, 811
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 1 cut(s) 782
DpnI GATC 5 cut(s) 379, 403, 421, 627, 756
DpnII GATC 5 cut(s) 377, 401, 419, 625, 754
DraIII CACNNNGTG 1 cut(s) 147
EaeI YGGCCR 3 cut(s) 71, 413, 648
Eam1104I CTCTTC 3 cut(s) 330, 615, 665
EarI CTCTTC 3 cut(s) 330, 615, 665
Eco130I CCWWGG 2 cut(s) 392, 584
Eco147I AGGCCT 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 612
Eco57I CTGAAG 3 cut(s) 304, 489, 681
Eco88I CYCGRG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 114, 415
EcoT14I CCWWGG 2 cut(s) 392, 584
EcoT22I ATGCAT 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 115
ErhI CCWWGG 2 cut(s) 392, 584
Esp3I CGTCTC 2 cut(s) 15, 190
FaeI CATG 6 cut(s) 356, 396, 588, 647, 692, 814
FaqI GGGAC 1 cut(s) 677
FatI CATG 6 cut(s) 352, 392, 584, 643, 688, 810
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 5 cut(s) 43, 127, 132, 398, 602
FokI GGATG 1 cut(s) 327
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 5 cut(s) 43, 127, 132, 398, 602
FspBI CTAG 2 cut(s) 306, 312
GlaI GCGC 2 cut(s) 130, 740
GluI GCNGC 5 cut(s) 43, 127, 132, 398, 602
HaeIII GGCC 4 cut(s) 73, 385, 415, 650
HapII CCGG 1 cut(s) 74
HgaI GACGC 2 cut(s) 154, 495
HhaI GCGC 2 cut(s) 131, 741
Hin1II CATG 6 cut(s) 356, 396, 588, 647, 692, 814
Hin6I GCGC 2 cut(s) 129, 739
HinP1I GCGC 2 cut(s) 129, 739
HindIII AAGCTT 3 cut(s) 265, 316, 452
HinfI GANTC 2 cut(s) 230, 589
HpaII CCGG 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 536
Hpy188I TCNGA 4 cut(s) 28, 170, 323, 617
Hpy188III TCNNGA 6 cut(s) 261, 293, 312, 431, 699, 716
Hpy8I GTNNAC 1 cut(s) 536
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 3 cut(s) 354, 513, 705
HpyCH4III ACNGT 2 cut(s) 705, 735
HpyCH4IV ACGT 1 cut(s) 143
HpyCH4V TGCA 7 cut(s) 174, 216, 493, 604, 668, 731, 827
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 171, 213, 382, 397, 460
HpyF3I CTNAG 1 cut(s) 782
HpySE526I ACGT 1 cut(s) 143
Hsp92II CATG 6 cut(s) 356, 396, 588, 647, 692, 814
HspAI GCGC 2 cut(s) 129, 739
KroI GCCGGC 1 cut(s) 73
KroNI GCCGGC 1 cut(s) 75
Kzo9I GATC 5 cut(s) 377, 401, 419, 625, 754
LguI GCTCTTC 2 cut(s) 330, 615
LmnI GCTCC 6 cut(s) 39, 110, 186, 514, 598, 852
Lsp1109I GCAGC 3 cut(s) 138, 143, 588
LweI GCATC 3 cut(s) 161, 349, 814
MaeI CTAG 2 cut(s) 306, 312
MaeII ACGT 1 cut(s) 143
MaeIII GTNAC 2 cut(s) 149, 424
MalI GATC 5 cut(s) 379, 403, 421, 627, 756
MbiI CCGCTC 1 cut(s) 189
MboI GATC 5 cut(s) 377, 401, 419, 625, 754
MboII GAAGA 6 cut(s) 249, 347, 515, 585, 602, 652
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 2 cut(s) 115, 141
MlsI TGGCCA 1 cut(s) 650
MluCI AATT 2 cut(s) 91, 564
MluNI TGGCCA 1 cut(s) 650
MnlI CCTC 9 cut(s) 54, 77, 132, 219, 300, 366, 664, 676, 683
Mox20I TGGCCA 1 cut(s) 650
Mph1103I ATGCAT 1 cut(s) 176
MroNI GCCGGC 1 cut(s) 73
MscI TGGCCA 1 cut(s) 650
MseI TTAA 2 cut(s) 555, 807
Msp20I TGGCCA 1 cut(s) 650
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 2 cut(s) 116, 417
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 2 cut(s) 116, 417
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 6 cut(s) 42, 171, 213, 382, 397, 460
NaeI GCCGGC 1 cut(s) 75
NcoI CCATGG 2 cut(s) 392, 584
NdeII GATC 5 cut(s) 377, 401, 419, 625, 754
NgoMIV GCCGGC 1 cut(s) 73
NlaIII CATG 6 cut(s) 356, 396, 588, 647, 692, 814
NlaIV GGNNCC 4 cut(s) 41, 112, 120, 848
NmuCI GTSAC 1 cut(s) 424
NsiI ATGCAT 1 cut(s) 176
NspI RCATGY 1 cut(s) 356
NspV TTCGAA 1 cut(s) 178
PceI AGGCCT 1 cut(s) 385
PciSI GCTCTTC 2 cut(s) 330, 615
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 225
PctI GAATGC 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 75
PfeI GAWTC 2 cut(s) 230, 589
PkrI GCNGC 5 cut(s) 44, 128, 133, 399, 603
Ple19I CGATCG 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 114, 415
PspGI CCWGG 2 cut(s) 114, 415
PspN4I GGNNCC 4 cut(s) 41, 112, 120, 848
PstI CTGCAG 1 cut(s) 606
PsuI RGATCY 1 cut(s) 625
PvuI CGATCG 1 cut(s) 404
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SapI GCTCTTC 2 cut(s) 330, 615
SaqAI TTAA 2 cut(s) 555, 807
SatI GCNGC 5 cut(s) 43, 127, 132, 398, 602
Sau3AI GATC 5 cut(s) 377, 401, 419, 625, 754
ScrFI CCNGG 2 cut(s) 116, 417
SduI GDGCHC 2 cut(s) 115, 141
SfaNI GCATC 3 cut(s) 161, 349, 814
SfcI CTRYAG 1 cut(s) 602
SfuI TTCGAA 1 cut(s) 178
Sse9I AATT 2 cut(s) 91, 564
SseBI AGGCCT 1 cut(s) 385
SsiI CCGC 4 cut(s) 43, 134, 189, 397
SspMI CTAG 2 cut(s) 306, 312
StuI AGGCCT 1 cut(s) 385
StyD4I CCNGG 2 cut(s) 114, 415
StyI CCWWGG 2 cut(s) 392, 584
TaaI ACNGT 2 cut(s) 705, 735
TaiI ACGT 1 cut(s) 146
TaqI TCGA 6 cut(s) 178, 228, 262, 675, 715, 757
TaqII GACCGA 1 cut(s) 636
TasI AATT 2 cut(s) 91, 564
TauI GCSGC 2 cut(s) 45, 400
TfiI GAWTC 2 cut(s) 230, 589
Tru1I TTAA 2 cut(s) 555, 807
Tru9I TTAA 2 cut(s) 555, 807
TscAI CASTG 3 cut(s) 478, 710, 834
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 3 cut(s) 126, 131, 601
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 4 cut(s) 495, 632, 652, 792
TspGWI ACGGA 2 cut(s) 385, 839
TspRI CASTG 3 cut(s) 478, 710, 834
XapI RAATTY 1 cut(s) 564
XbaI TCTAGA 1 cut(s) 311
XceI RCATGY 1 cut(s) 356
XspI CTAG 2 cut(s) 306, 312
Zsp2I ATGCAT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.