Rh4CG246600

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010256
Physical Location & Seq
Forward (+)
49592444 .. 49595738
3295 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG246600.1

Sequence Viewer

Length: 768 bp
ATGGAATCGTCAATCGCTGCACCGCCGGCGACAAGAAGCAGCCGCGAGAAGCTGGTACAGAGAGTAATCTTGCCGGCGATCCGAGGGCAAAACTGTCCAATATGCTTAGGAAGCCTCGAGCCTCGCAGCGCGGCGGTGCTGGAGGCGTGTAAGCACGCGTACTGCCTCCGATGCATTCGCAGGTGGAGCACTTTGAGACGAAAATGCCCTCTCTGTAATTCCAACTTCGACTCTTGGTTCCATAACTTCACCCTTTCTTCTCCAAATTTCCACACACAGATTTTACCACCGCTCAATACTAACGCTTCTCGAACTAGAAGCGGCGAACATCCAAGGTATGTGATGAATGAGGGGCGGCGATCACGGCCGTTGCCATGGCGGCGGTCGTTTGGACGGCCAGGTTCTGTGGCTGCAGATGTGATCACTCAGAGGAAACTTCAGTGGCGTGCAGGTATATATGGCAGACGTTTGCAAGCTGTTGCTTCAGCTCCAAGAAACCGTGTTCGAGTGTGTGTGCCGGTTAACGATGTGGTGAAGGAGAGAGTGTTGCAAAGAGTAGAACCTTGGATTAGAAGAGAGCTGCAGGTTCTTCTTGGAGACCGAGATCCATCTGTTGTTGTTCATGTGGCCACCTCGCTATTCATTGCAAGCCTTGAAAGGAAGGATTTTGTCTCTTCAGAGCAACGTAATGTACTAGATAACTTCCTTGAGCCGTTGCGCCCTTTCCTCCTCGATCGAACTGATATGTTTTGGCACGAATTAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0000922 GO:0000930 GO:0001654 GO:0001754 GO:0003407 GO:0003674 GO:0003676 GO:0003677 GO:0003823 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005814 GO:0005815 GO:0005819 GO:0005856 GO:0005929 GO:0006139 GO:0006351 GO:0006464 GO:0006508 GO:0006511 GO:0006513 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009653 GO:0009887 GO:0009893 GO:0009987 GO:0010467 GO:0010498 GO:0010604 GO:0010842 GO:0010941 GO:0012501 GO:0015630 GO:0016043 GO:0016070 GO:0016567 GO:0016604 GO:0016605 GO:0016607 GO:0016740 GO:0016925 GO:0018130 GO:0018193 GO:0018205 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019789 GO:0019899 GO:0019941 GO:0022008 GO:0023052 GO:0030154 GO:0030163 GO:0030182 GO:0030496 GO:0031323 GO:0031325 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032391 GO:0032446 GO:0032501 GO:0032502 GO:0032507 GO:0032774 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035556 GO:0035845 GO:0035869 GO:0036064 GO:0036211 GO:0042461 GO:0042462 GO:0042670 GO:0042981 GO:0042995 GO:0043005 GO:0043010 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044451 GO:0044463 GO:0044464 GO:0044547 GO:0045185 GO:0046483 GO:0046530 GO:0046548 GO:0046549 GO:0048468 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048592 GO:0048593 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051235 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051438 GO:0051443 GO:0051457 GO:0051603 GO:0051641 GO:0051651 GO:0051716 GO:0060041 GO:0060042 GO:0060219 GO:0060255 GO:0060548 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070647 GO:0070727 GO:0070936 GO:0071704 GO:0071840 GO:0072595 GO:0080090 GO:0090304 GO:0090596 GO:0097159 GO:0097190 GO:0097193 GO:0097458 GO:0097659 GO:0097730 GO:0097731 GO:0097733 GO:0120025 GO:0120038 GO:0140096 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1902494 GO:1903320 GO:1903322 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWI zf-C3HC4 zf-C3HC4_3 zf-RING_2

Protein Analysis

255

Amino Acids

29.41

Weight (kDa)

10.96

Isoelectric Point (pI)

66.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 30 - 73 2.3e-06 RING-H2 zinc finger domain
zf-C3HC4_3 PF13920 30 - 77 2.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 31 - 73 2.6e-09 Ring finger domain
zf-C3HC4_2 PF13923 31 - 72 8.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 31 - 73 3.2e-07 zinc-RING finger domain
zf-C3HC4 PF00097 32 - 72 5.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 32 - 70 9.7e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 171
Acc36I ACCTGC 3 cut(s) 171, 440, 574
AccBSI CCGCTC 1 cut(s) 292
AccII CGCG 3 cut(s) 45, 131, 158
AciI CCGC 9 cut(s) 23, 43, 131, 134, 290, 321, 355, 379, 382
AclWI GGATC 2 cut(s) 73, 599
AcoI YGGCCR 3 cut(s) 365, 395, 627
AcsI RAATTY 1 cut(s) 265
AcuI CTGAAG 3 cut(s) 422, 468, 660
AfaI GTAC 3 cut(s) 57, 161, 693
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 1 cut(s) 656
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 4 cut(s) 52, 476, 488, 580
AluI AGCT 4 cut(s) 52, 476, 488, 580
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 3 cut(s) 190, 591, 676
AlwI GGATC 2 cut(s) 73, 599
AlwNI CAGNNNCTG 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 3 cut(s) 365, 395, 627
ApeKI GCWGC 5 cut(s) 17, 39, 126, 410, 580
ApoI RAATTY 1 cut(s) 265
AspLEI GCGC 2 cut(s) 131, 720
AsuHPI GGTGA 2 cut(s) 241, 544
AvaI CYCGRG 1 cut(s) 116
BalI TGGCCA 1 cut(s) 629
Bbv12I GWGCWC 1 cut(s) 191
BbvI GCAGC 5 cut(s) 4, 51, 138, 397, 567
BccI CCATC 1 cut(s) 616
BceAI ACGGC 4 cut(s) 352, 380, 410, 697
BciT130I CCWGG 1 cut(s) 399
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 3 cut(s) 190, 591, 676
BfaI CTAG 2 cut(s) 315, 695
BfmI CTRYAG 2 cut(s) 411, 581
BfuAI ACCTGC 3 cut(s) 171, 440, 574
BglI GCCNNNNNGGC 1 cut(s) 379
Bme1390I CCNGG 1 cut(s) 399
BmeT110I CYCGRG 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 161
Bpu10I CCTNAGC 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 728
BsaI GGTCTC 1 cut(s) 591
BsaJI CCNNGG 4 cut(s) 82, 332, 374, 563
BsaXI ACNNNNNCTCC 2 cut(s) 530, 560
Bse118I RCCGGY 3 cut(s) 25, 73, 517
Bse3DI GCAATG 1 cut(s) 642
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 4 cut(s) 82, 332, 374, 563
BseGI GGATG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 642
BseMII CTCAG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 719
BseX3I CGGCCG 1 cut(s) 365
BseXI GCAGC 5 cut(s) 4, 51, 138, 397, 567
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 3 cut(s) 45, 131, 158
Bsh1285I CGRYCG 3 cut(s) 368, 386, 736
BshFI GGCC 3 cut(s) 367, 397, 629
BsiEI CGRYCG 3 cut(s) 368, 386, 736
BsiHKAI GWGCWC 1 cut(s) 191
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 3 cut(s) 26, 74, 518
BsmAI GTCTC 3 cut(s) 190, 591, 676
BsmBI CGTCTC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 3 cut(s) 367, 397, 629
Bso31I GGTCTC 1 cut(s) 591
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 191
Bsp143I GATC 5 cut(s) 78, 359, 420, 604, 733
Bsp19I CCATGG 1 cut(s) 374
BspACI CCGC 9 cut(s) 23, 43, 131, 134, 290, 321, 355, 379, 382
BspANI GGCC 3 cut(s) 367, 397, 629
BspCNI CTCAG 1 cut(s) 439
BspFNI CGCG 3 cut(s) 45, 131, 158
BspLI GGNNCC 1 cut(s) 239
BspMAI CTGCAG 2 cut(s) 415, 585
BspMI ACCTGC 3 cut(s) 171, 440, 574
BspPI GGATC 2 cut(s) 73, 599
BspTNI GGTCTC 1 cut(s) 591
BsrBI CCGCTC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 642
BsrFI RCCGGY 3 cut(s) 25, 73, 517
BssAI RCCGGY 3 cut(s) 25, 73, 517
BssECI CCNNGG 4 cut(s) 82, 332, 374, 563
BssMI GATC 5 cut(s) 78, 359, 420, 604, 733
BssT1I CCWWGG 3 cut(s) 332, 374, 563
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 2 cut(s) 95, 500
Bst6I CTCTTC 2 cut(s) 568, 679
BstC8I GCNNGC 6 cut(s) 27, 75, 156, 447, 474, 649
BstDEI CTNAG 2 cut(s) 106, 426
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 1 cut(s) 328
BstFNI CGCG 3 cut(s) 45, 131, 158
BstHHI GCGC 2 cut(s) 131, 720
BstKTI GATC 5 cut(s) 81, 362, 423, 607, 736
BstMAI GTCTC 3 cut(s) 190, 591, 676
BstMBI GATC 5 cut(s) 78, 359, 420, 604, 733
BstMCI CGRYCG 3 cut(s) 368, 386, 736
BstMWI GCNNNNNNNGC 6 cut(s) 26, 111, 171, 186, 364, 379
BstNI CCWGG 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 2 cut(s) 411, 581
BstUI CGCG 3 cut(s) 45, 131, 158
BstV1I GCAGC 5 cut(s) 4, 51, 138, 397, 567
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BstZI CGGCCG 1 cut(s) 365
BsuRI GGCC 3 cut(s) 367, 397, 629
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 1 cut(s) 328
BtsIMutI CAGTG 1 cut(s) 446
BveI ACCTGC 3 cut(s) 171, 440, 574
Cac8I GCNNGC 6 cut(s) 27, 75, 156, 447, 474, 649
CaiI CAGNNNCTG 1 cut(s) 404
CfoI GCGC 2 cut(s) 131, 720
Cfr10I RCCGGY 3 cut(s) 25, 73, 517
Csp6I GTAC 3 cut(s) 56, 160, 692
CviAII CATG 2 cut(s) 375, 623
CviQI GTAC 3 cut(s) 56, 160, 692
DdeI CTNAG 2 cut(s) 106, 426
DpnI GATC 5 cut(s) 80, 361, 422, 606, 735
DpnII GATC 5 cut(s) 78, 359, 420, 604, 733
EaeI YGGCCR 3 cut(s) 365, 395, 627
EagI CGGCCG 1 cut(s) 365
Eam1104I CTCTTC 2 cut(s) 568, 679
EarI CTCTTC 2 cut(s) 568, 679
EclXI CGGCCG 1 cut(s) 365
Eco130I CCWWGG 3 cut(s) 332, 374, 563
Eco31I GGTCTC 1 cut(s) 591
Eco52I CGGCCG 1 cut(s) 365
Eco57I CTGAAG 3 cut(s) 422, 468, 660
Eco88I CYCGRG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 397
EcoT14I CCWWGG 3 cut(s) 332, 374, 563
EcoT22I ATGCAT 1 cut(s) 176
ErhI CCWWGG 3 cut(s) 332, 374, 563
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 2 cut(s) 378, 626
FaiI YATR 9 cut(s) 103, 243, 339, 376, 455, 457, 459, 624, 746
FatI CATG 2 cut(s) 374, 622
FbaI TGATCA 1 cut(s) 420
FokI GGATG 1 cut(s) 315
FspBI CTAG 2 cut(s) 315, 695
GlaI GCGC 2 cut(s) 130, 719
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 3 cut(s) 367, 397, 629
HapII CCGG 3 cut(s) 26, 74, 518
HhaI GCGC 2 cut(s) 131, 720
Hin1II CATG 2 cut(s) 378, 626
Hin6I GCGC 2 cut(s) 129, 718
HinP1I GCGC 2 cut(s) 129, 718
HincII GTYRAC 1 cut(s) 523
HindII GTYRAC 1 cut(s) 523
HinfI GANTC 2 cut(s) 5, 230
HpaI GTTAAC 1 cut(s) 523
HpaII CCGG 3 cut(s) 26, 74, 518
HphI GGTGA 2 cut(s) 241, 544
Hpy166II GTNNAC 1 cut(s) 523
Hpy188I TCNGA 4 cut(s) 83, 170, 429, 679
Hpy188III TCNNGA 1 cut(s) 309
Hpy8I GTNNAC 1 cut(s) 523
HpyAV CCTTC 2 cut(s) 529, 655
HpyCH4III ACNGT 2 cut(s) 95, 500
HpyCH4IV ACGT 2 cut(s) 466, 685
HpyCH4V TGCA 8 cut(s) 20, 174, 413, 449, 472, 550, 583, 647
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 111, 171, 186, 364, 379
HpyF3I CTNAG 2 cut(s) 106, 426
HpySE526I ACGT 2 cut(s) 466, 685
Hsp92II CATG 2 cut(s) 378, 626
HspAI GCGC 2 cut(s) 129, 718
KroI GCCGGC 2 cut(s) 25, 73
KroNI GCCGGC 2 cut(s) 27, 75
Ksp22I TGATCA 1 cut(s) 420
KspAI GTTAAC 1 cut(s) 523
Kzo9I GATC 5 cut(s) 78, 359, 420, 604, 733
LmnI GCTCC 2 cut(s) 186, 493
Lsp1109I GCAGC 5 cut(s) 4, 51, 138, 397, 567
LweI GCATC 1 cut(s) 161
MaeI CTAG 2 cut(s) 315, 695
MaeII ACGT 2 cut(s) 466, 685
MalI GATC 5 cut(s) 80, 361, 422, 606, 735
MbiI CCGCTC 1 cut(s) 292
MboI GATC 5 cut(s) 78, 359, 420, 604, 733
MboII GAAGA 4 cut(s) 249, 581, 585, 666
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 1 cut(s) 191
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 3 cut(s) 217, 265, 758
MluI ACGCGT 1 cut(s) 156
MluNI TGGCCA 1 cut(s) 629
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 246
Mox20I TGGCCA 1 cut(s) 629
Mph1103I ATGCAT 1 cut(s) 176
MreI CGCCGGCG 1 cut(s) 25
MroNI GCCGGC 2 cut(s) 25, 73
MscI TGGCCA 1 cut(s) 629
MseI TTAA 2 cut(s) 522, 761
Msp20I TGGCCA 1 cut(s) 629
MspI CCGG 3 cut(s) 26, 74, 518
MspR9I CCNGG 1 cut(s) 399
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 3 cut(s) 45, 131, 158
MwoI GCNNNNNNNGC 6 cut(s) 26, 111, 171, 186, 364, 379
NaeI GCCGGC 2 cut(s) 27, 75
NcoI CCATGG 1 cut(s) 374
NdeII GATC 5 cut(s) 78, 359, 420, 604, 733
NgoMIV GCCGGC 2 cut(s) 25, 73
NlaIII CATG 2 cut(s) 378, 626
NlaIV GGNNCC 1 cut(s) 239
NsiI ATGCAT 1 cut(s) 176
PaeR7I CTCGAG 1 cut(s) 116
PaqCI CACCTGC 1 cut(s) 171
PctI GAATGC 1 cut(s) 174
PdiI GCCGGC 2 cut(s) 27, 75
PfeI GAWTC 1 cut(s) 5
Ple19I CGATCG 1 cut(s) 736
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 1 cut(s) 239
PspXI VCTCGAGB 1 cut(s) 116
PstI CTGCAG 2 cut(s) 415, 585
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 1 cut(s) 604
PvuI CGATCG 1 cut(s) 736
RsaI GTAC 3 cut(s) 57, 161, 693
RsaNI GTAC 3 cut(s) 56, 160, 692
SaqAI TTAA 2 cut(s) 522, 761
Sau3AI GATC 5 cut(s) 78, 359, 420, 604, 733
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 1 cut(s) 191
SfaNI GCATC 1 cut(s) 161
SfcI CTRYAG 2 cut(s) 411, 581
Sfr274I CTCGAG 1 cut(s) 116
SgrAI CRCCGGYG 1 cut(s) 25
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 2 cut(s) 116, 707
SmoI CTYRAG 2 cut(s) 116, 707
Sse9I AATT 3 cut(s) 217, 265, 758
SsiI CCGC 9 cut(s) 23, 43, 131, 134, 290, 321, 355, 379, 382
SspMI CTAG 2 cut(s) 315, 695
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 3 cut(s) 332, 374, 563
TaaI ACNGT 2 cut(s) 95, 500
TaiI ACGT 2 cut(s) 469, 688
TaqI TCGA 6 cut(s) 117, 228, 310, 505, 732, 736
TaqII GACCGA 1 cut(s) 615
TasI AATT 3 cut(s) 217, 265, 758
TatI WGTACW 1 cut(s) 691
TauI GCSGC 5 cut(s) 45, 134, 324, 358, 382
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 522, 761
Tru9I TTAA 2 cut(s) 522, 761
TscAI CASTG 1 cut(s) 446
TseI GCWGC 5 cut(s) 17, 39, 126, 410, 580
TspDTI ATGAA 3 cut(s) 359, 611, 631
TspRI CASTG 1 cut(s) 446
XapI RAATTY 1 cut(s) 265
XhoI CTCGAG 1 cut(s) 116
XspI CTAG 2 cut(s) 315, 695
Zsp2I ATGCAT 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.