Prupe.8G004900_v2.0.a1

DNA topoisomerase binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0010256
Physical Location & Seq
Forward (+)
391598 .. 391918
321 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G004900.1

Sequence Viewer

Length: 321 bp
ATGTGCAACGAAGGGAAAAATAGAGAGAGCCCGCCAAATCACTCTCCACACCCACACAACCCGAAATGGCGTGTCAGTGTATATGAAAGACGCTTGCAAGCTGTTCCTTCAGCTCCAAGAAGTCGTCTTCAAGTGAGTGTACCAATAAATGATGGTGTTAAAGAGAGAATTTTGCGAAGAATAGAACCATGGATTCGTAGGGAGCTGCAGGCTCTTCTCGGAGACCGAGATCCATCTATTATTGTTCATGTGGCCACCTCACTCTTCATTGCAAGCCTCGAAAACGAGGGTCATGTCCCTTCAGGACAGTGTGATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000209 GO:0000922 GO:0000930 GO:0001654 GO:0001754 GO:0003407 GO:0003674 GO:0003676 GO:0003677 GO:0003823 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005814 GO:0005815 GO:0005819 GO:0005856 GO:0005929 GO:0006139 GO:0006351 GO:0006464 GO:0006508 GO:0006511 GO:0006513 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0007399 GO:0007423 GO:0008104 GO:0008150 GO:0008152 GO:0008219 GO:0008630 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009653 GO:0009887 GO:0009893 GO:0009987 GO:0010467 GO:0010498 GO:0010604 GO:0010842 GO:0010941 GO:0012501 GO:0015630 GO:0016043 GO:0016070 GO:0016567 GO:0016604 GO:0016605 GO:0016607 GO:0016740 GO:0016925 GO:0018130 GO:0018193 GO:0018205 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019789 GO:0019899 GO:0019941 GO:0022008 GO:0023052 GO:0030154 GO:0030163 GO:0030182 GO:0030496 GO:0031323 GO:0031325 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032391 GO:0032446 GO:0032501 GO:0032502 GO:0032507 GO:0032774 GO:0032991 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0035556 GO:0035845 GO:0035869 GO:0036064 GO:0036211 GO:0042461 GO:0042462 GO:0042670 GO:0042981 GO:0042995 GO:0043005 GO:0043010 GO:0043066 GO:0043067 GO:0043069 GO:0043085 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043632 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044450 GO:0044451 GO:0044463 GO:0044464 GO:0044547 GO:0045185 GO:0046483 GO:0046530 GO:0046548 GO:0046549 GO:0048468 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048592 GO:0048593 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051179 GO:0051235 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051438 GO:0051443 GO:0051457 GO:0051603 GO:0051641 GO:0051651 GO:0051716 GO:0060041 GO:0060042 GO:0060219 GO:0060255 GO:0060548 GO:0061630 GO:0061659 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070647 GO:0070727 GO:0070936 GO:0071704 GO:0071840 GO:0072595 GO:0080090 GO:0090304 GO:0090596 GO:0097159 GO:0097190 GO:0097193 GO:0097458 GO:0097659 GO:0097730 GO:0097731 GO:0097733 GO:0120025 GO:0120038 GO:0140096 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1902494 GO:1903320 GO:1903322 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWI zf-RING_2

Protein Analysis

107

Amino Acids

12.04

Weight (kDa)

9.29

Isoelectric Point (pI)

72.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 32
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 168
AcuI CTGAAG 2 cut(s) 93, 285
AfaI GTAC 1 cut(s) 141
AgsI TTSAA 1 cut(s) 131
AluBI AGCT 3 cut(s) 101, 113, 205
AluI AGCT 3 cut(s) 101, 113, 205
Alw26I GTCTC 1 cut(s) 216
AlwI GGATC 1 cut(s) 224
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 168
BalI TGGCCA 1 cut(s) 254
BanII GRGCYC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 192
BccI CCATC 2 cut(s) 146, 241
BcoDI GTCTC 1 cut(s) 216
BfmI CTRYAG 1 cut(s) 206
BisI GCNGC 1 cut(s) 206
BlsI GCNGC 1 cut(s) 207
BpiI GAAGAC 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 216
BsaJI CCNNGG 1 cut(s) 188
Bse3DI GCAATG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 188
BseMI GCAATG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 192
BshFI GGCC 1 cut(s) 254
BslFI GGGAC 1 cut(s) 281
BsmAI GTCTC 1 cut(s) 216
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 1 cut(s) 254
Bso31I GGTCTC 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 1 cut(s) 229
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 1 cut(s) 32
BspANI GGCC 1 cut(s) 254
BspMAI CTGCAG 1 cut(s) 210
BspPI GGATC 1 cut(s) 224
BspQI GCTCTTC 1 cut(s) 219
BspTNI GGTCTC 1 cut(s) 216
BsrDI GCAATG 1 cut(s) 267
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 1 cut(s) 229
BssT1I CCWWGG 1 cut(s) 188
Bst4CI ACNGT 1 cut(s) 309
Bst6I CTCTTC 2 cut(s) 219, 269
BstC8I GCNNGC 5 cut(s) 32, 95, 99, 210, 274
BstDSI CCRYGG 1 cut(s) 188
BstKTI GATC 1 cut(s) 232
BstMAI GTCTC 1 cut(s) 216
BstMBI GATC 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 192
BstV2I GAAGAC 1 cut(s) 119
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 1 cut(s) 254
BtgI CCRYGG 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 82, 314
Cac8I GCNNGC 5 cut(s) 32, 95, 99, 210, 274
CseI GACGC 1 cut(s) 99
Csp6I GTAC 1 cut(s) 140
CviAII CATG 3 cut(s) 189, 248, 293
CviJI RGCY 7 cut(s) 30, 101, 113, 205, 212, 254, 276
CviKI_1 RGCY 7 cut(s) 30, 101, 113, 205, 212, 254, 276
CviQI GTAC 1 cut(s) 140
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 219, 269
EarI CTCTTC 2 cut(s) 219, 269
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 216
Eco57I CTGAAG 2 cut(s) 93, 285
EcoT14I CCWWGG 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 32
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 3 cut(s) 192, 251, 296
FaiI YATR 5 cut(s) 82, 84, 190, 249, 294
FaqI GGGAC 1 cut(s) 281
FatI CATG 3 cut(s) 188, 247, 292
FauI CCCGC 1 cut(s) 39
Fnu4HI GCNGC 1 cut(s) 206
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 1 cut(s) 206
GluI GCNGC 1 cut(s) 206
HaeIII GGCC 1 cut(s) 254
HgaI GACGC 1 cut(s) 99
Hin1II CATG 3 cut(s) 192, 251, 296
HinfI GANTC 1 cut(s) 193
Hpy166II GTNNAC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 221
Hpy188III TCNNGA 1 cut(s) 303
Hpy8I GTNNAC 1 cut(s) 140
HpyAV CCTTC 3 cut(s) 5, 117, 309
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4V TGCA 4 cut(s) 6, 97, 208, 272
Hsp92II CATG 3 cut(s) 192, 251, 296
Kzo9I GATC 1 cut(s) 229
LguI GCTCTTC 1 cut(s) 219
LmnI GCTCC 2 cut(s) 118, 202
LpnPI CCDG 2 cut(s) 194, 288
Lsp1109I GCAGC 1 cut(s) 192
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 4 cut(s) 119, 189, 206, 256
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 1 cut(s) 168
MluNI TGGCCA 1 cut(s) 254
MnlI CCTC 3 cut(s) 268, 280, 287
Mox20I TGGCCA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 254
MseI TTAA 1 cut(s) 159
Msp20I TGGCCA 1 cut(s) 254
NcoI CCATGG 1 cut(s) 188
NdeII GATC 1 cut(s) 229
NlaIII CATG 3 cut(s) 192, 251, 296
PciSI GCTCTTC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 193
PkrI GCNGC 1 cut(s) 207
PstI CTGCAG 1 cut(s) 210
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 219
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 1 cut(s) 206
Sau3AI GATC 1 cut(s) 229
SduI GDGCHC 1 cut(s) 32
SetI ASST 4 cut(s) 103, 115, 207, 260
SfcI CTRYAG 1 cut(s) 206
Sse9I AATT 1 cut(s) 168
SsiI CCGC 1 cut(s) 32
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 309
TaqI TCGA 1 cut(s) 279
TaqII GACCGA 1 cut(s) 240
TasI AATT 1 cut(s) 168
TfiI GAWTC 1 cut(s) 193
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 2 cut(s) 82, 314
TseI GCWGC 1 cut(s) 205
TspDTI ATGAA 3 cut(s) 99, 236, 256
TspRI CASTG 2 cut(s) 82, 314
XapI RAATTY 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.