Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012607

Physical Location & Seq

Genomic Coordinates Reverse (-)

14366354 .. 14369937

Gene Structure 3584 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1174400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGACGACGGCGCCAGAGCTTCTGGAAGTTCATCCCCGCGAGCTCAAATTCACATTTGAAGTGAAGAAGCAGAGTACGTGCTCGATTCAACTCGGAAACAAGTCCGACCACTATGTTGCTTTCAAGGTGAAAACCACTTCCCCAAAGAAATATTGTGTGCGGCCGAATGCAGGCATTGTAAAGCCCAAGACAACGTGTGATTTTACAGTTACTATGCAAGCTCAGCGTGTTGCTCCACCTGATTTGCAGTGCAAAGACAAGTTCCTAATCCAGTGCACCGTTATTCCATTTGGGACAACTGAACAGGAGATAACATCCGAGATGTTTTCAAAAGGTAGTGGACGATACATTGAAGAAAGGAAGCTAAAAGTGGTGCTCGTTAGCCCGCCCCCATCCCCAGTATTTGTACCAGTTAATGGGGAGTCGAAGCAAGATCCATGCTATGAAACATCGGTGAAAAAGGATAGAGTTCTGACTGAAGTTGAAAACATACCTCCGAGTCATGGGGTTGCTGAGGATGTTGACGTGTTTGAAACTTGTAAGGACATGGATGAGTCAAGAGCAGTTAAGGATGTGGTGGCATCGAAACCAGCTAAGGATGCGCAGGAATTGAAACCAGATAAGGATGTGGAGGAATCAAAACCAGCTAAACATATGGAGGAACTGAAACCAGTGAAAAATATGGAGGAACCAAATCCAACGAAAGATATGGACGAGTCGAAACCAGCCAAGGATACGGAGGAATTGAAACCAACTAGGGGTGCTGCAGTACTAAATTTAACAAAGGATTTTGAGGAACTGAAATCAAAGCTAAATACGGTGGATTCAAAGCTAAAAGAGGCTGAACTCACCATCACGAAGTTAACTGAAGAGAGTAGCAGGTCCTCTCGAGAGAAGAGTATGCTTAAACATGAATTGGAATCGTTGAGGAGGAGGAAGAACAATTTAAAAAGGGTTACGGTTGGCTTCCCTCTCTTTCATGTTTGTATGGTTGCCCTTATCAGTCTAGCATTCGGATACTACATCCATCCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0008150 GO:0012505 GO:0016020 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0071944 GO:0098827

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Motile_Sperm

Protein Analysis

345

Amino Acids

38.8

Weight (kDa)

7.99

Isoelectric Point (pI)

46.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Motile_Sperm	PF00635	8 - 113	4.1e-31	MSP (Major sperm protein) domain
Cep192_D6	PF22076	8 - 89	1.1e-06	Cep192 domain 6

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012607)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g17100 FvH4_4g17100 FvH4_4g17100
malus_domestica	MD13G1174400.v1.1 MD16G1175900.v1.1
prunus_persica	Prupe.1G142800_v2.0.a1 Prupe.1G142800_v2.0.a1
pyrus_communis	pycom13g15000 pycom16g14740
rosa_chinensis	RchiOBHm_Chr4g0420891
rosa_laevigata	RLG00000007727
rosa_multiflora	Rmu_sc0002006.1_g000007
rosa_roxburghii	Rroxscaffold_5G00363290
rosa_rugosa	Rorug04G0166700
rosa_samantha	Rh4AG228100 Rh4BG231200 Rh4CG243100 Rh4DG226400
rosa_wichuraiana	Rw4G019750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	603
Acc36I	ACCTGC	1 cut(s)	870
AccB1I	GGYRCC	1 cut(s)	10
AccB7I	CCANNNNNTGG	1 cut(s)	416
AccII	CGCG	1 cut(s)	39
AciI	CCGC	3 cut(s)	37, 160, 386
AclWI	GGATC	1 cut(s)	428
AcoI	YGGCCR	1 cut(s)	161
AcsI	RAATTY	2 cut(s)	47, 775
AcuI	CTGAAG	2 cut(s)	498, 888
AcyI	GRCGYC	1 cut(s)	11
AfaI	GTAC	3 cut(s)	76, 408, 771
AfiI	CCNNNNNNNGG	4 cut(s)	170, 416, 503, 758
AflIII	ACRYGT	2 cut(s)	194, 525
AjiI	CACGTC	1 cut(s)	526
AluBI	AGCT	8 cut(s)	19, 43, 221, 364, 593, 647, 811, 832
AluI	AGCT	8 cut(s)	19, 43, 221, 364, 593, 647, 811, 832
Alw21I	GWGCWC	4 cut(s)	45, 83, 278, 378
Alw44I	GTGCAC	1 cut(s)	274
AlwI	GGATC	1 cut(s)	428
Ama87I	CYCGRG	1 cut(s)	888
AoxI	GGCC	1 cut(s)	161
ApaLI	GTGCAC	1 cut(s)	274
ApeKI	GCWGC	1 cut(s)	764
ApoI	RAATTY	2 cut(s)	47, 775
AspLEI	GCGC	2 cut(s)	13, 604
AspS9I	GGNCC	1 cut(s)	882
AsuHPI	GGTGA	3 cut(s)	139, 466, 841
AvaI	CYCGRG	1 cut(s)	888
AvaII	GGWCC	1 cut(s)	882
BaeGI	GKGCMC	1 cut(s)	278
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	1 cut(s)	45
Bbv12I	GWGCWC	4 cut(s)	45, 83, 278, 378
BbvCI	CCTCAGC	1 cut(s)	513
BbvI	GCAGC	1 cut(s)	751
BccI	CCATC	2 cut(s)	400, 860
BceAI	ACGGC	1 cut(s)	24
BciVI	GTATCC	2 cut(s)	727, 1010
BfaI	CTAG	2 cut(s)	756, 1007
BfmI	CTRYAG	1 cut(s)	765
BfoI	RGCGCY	1 cut(s)	14
BfuAI	ACCTGC	1 cut(s)	870
BfuI	GTATCC	2 cut(s)	727, 1010
BisI	GCNGC	2 cut(s)	161, 765
BlpI	GCTNAGC	1 cut(s)	222
BlsI	GCNGC	2 cut(s)	162, 766
BmcAI	AGTACT	1 cut(s)	771
Bme18I	GGWCC	1 cut(s)	882
BmeT110I	CYCGRG	1 cut(s)	888
BmgBI	CACGTC	1 cut(s)	526
BmgT120I	GGNCC	1 cut(s)	882
BmiI	GGNNCC	2 cut(s)	12, 690
BmrI	ACTGGG	1 cut(s)	392
BmsI	GCATC	2 cut(s)	589, 590
BmuI	ACTGGG	1 cut(s)	392
Bpu10I	CCTNAGC	2 cut(s)	513, 594
Bpu1102I	GCTNAGC	1 cut(s)	222
BsaAI	YACGTR	1 cut(s)	78
BsaHI	GRCGYC	1 cut(s)	11
BsaJI	CCNNGG	1 cut(s)	729
Bsc4I	CCNNNNNNNGG	4 cut(s)	170, 416, 503, 758
Bse1I	ACTGG	4 cut(s)	271, 398, 410, 671
BseDI	CCNNGG	1 cut(s)	729
BseLI	CCNNNNNNNGG	4 cut(s)	170, 416, 503, 758
BseMII	CTCAG	2 cut(s)	236, 504
BseNI	ACTGG	4 cut(s)	271, 398, 410, 671
BseRI	GAGGAG	2 cut(s)	943, 946
BseSI	GKGCMC	1 cut(s)	278
BseX3I	CGGCCG	1 cut(s)	161
BseXI	GCAGC	1 cut(s)	751
Bsh1236I	CGCG	1 cut(s)	39
Bsh1285I	CGRYCG	1 cut(s)	164
BshFI	GGCC	1 cut(s)	163
BshNI	GGYRCC	1 cut(s)	10
BsiEI	CGRYCG	1 cut(s)	164
BsiHKAI	GWGCWC	4 cut(s)	45, 83, 278, 378
BsiHKCI	CYCGRG	1 cut(s)	888
BslFI	GGGAC	1 cut(s)	307
BslI	CCNNNNNNNGG	4 cut(s)	170, 416, 503, 758
BsmFI	GGGAC	1 cut(s)	307
BsmI	GAATGC	2 cut(s)	172, 1010
BsnI	GGCC	1 cut(s)	163
BsoBI	CYCGRG	1 cut(s)	888
Bsp1286I	GDGCHC	4 cut(s)	45, 83, 278, 378
Bsp143I	GATC	1 cut(s)	433
Bsp1720I	GCTNAGC	1 cut(s)	222
BspACI	CCGC	3 cut(s)	37, 160, 386
BspANI	GGCC	1 cut(s)	163
BspCNI	CTCAG	2 cut(s)	235, 505
BspFNI	CGCG	1 cut(s)	39
BspLI	GGNNCC	2 cut(s)	12, 690
BspMAI	CTGCAG	1 cut(s)	769
BspMI	ACCTGC	1 cut(s)	870
BspPI	GGATC	1 cut(s)	428
BspT107I	GGYRCC	1 cut(s)	10
BsrI	ACTGG	4 cut(s)	271, 398, 410, 671
BssECI	CCNNGG	1 cut(s)	729
BssMI	GATC	1 cut(s)	433
BssNI	GRCGYC	1 cut(s)	11
BssT1I	CCWWGG	1 cut(s)	729
Bst4CI	ACNGT	4 cut(s)	208, 280, 820, 961
Bst6I	CTCTTC	2 cut(s)	864, 890
BstACI	GRCGYC	1 cut(s)	11
BstBAI	YACGTR	1 cut(s)	78
BstC8I	GCNNGC	4 cut(s)	41, 172, 219, 386
BstDEI	CTNAG	3 cut(s)	222, 513, 594
BstFNI	CGCG	1 cut(s)	39
BstH2I	RGCGCY	1 cut(s)	14
BstHHI	GCGC	2 cut(s)	13, 604
BstKTI	GATC	1 cut(s)	436
BstMBI	GATC	1 cut(s)	433
BstMCI	CGRYCG	1 cut(s)	164
BstMWI	GCNNNNNNNGC	2 cut(s)	223, 599
BstSFI	CTRYAG	1 cut(s)	765
BstSLI	GKGCMC	1 cut(s)	278
BstUI	CGCG	1 cut(s)	39
BstV1I	GCAGC	1 cut(s)	751
BstX2I	RGATCY	1 cut(s)	433
BstYI	RGATCY	1 cut(s)	433
BstZI	CGGCCG	1 cut(s)	161
BsuI	GTATCC	2 cut(s)	727, 1010
BsuRI	GGCC	1 cut(s)	163
BtrI	CACGTC	1 cut(s)	526
BtsI	GCAGTG	1 cut(s)	254
BtsIMutI	CAGTG	3 cut(s)	254, 278, 678
BveI	ACCTGC	1 cut(s)	870
Cac8I	GCNNGC	4 cut(s)	41, 172, 219, 386
CfoI	GCGC	2 cut(s)	13, 604
Cfr13I	GGNCC	1 cut(s)	882
Csp6I	GTAC	3 cut(s)	75, 407, 770
CspCI	CAANNNNNGTGG	2 cut(s)	225, 260
CviAII	CATG	5 cut(s)	438, 503, 547, 911, 980
CviQI	GTAC	3 cut(s)	75, 407, 770
DdeI	CTNAG	3 cut(s)	222, 513, 594
DinI	GGCGCC	1 cut(s)	12
DpnI	GATC	1 cut(s)	435
DpnII	GATC	1 cut(s)	433
DraI	TTTAAA	1 cut(s)	948
EaeI	YGGCCR	1 cut(s)	161
EagI	CGGCCG	1 cut(s)	161
Eam1104I	CTCTTC	2 cut(s)	864, 890
EarI	CTCTTC	2 cut(s)	864, 890
Ecl136II	GAGCTC	1 cut(s)	43
EclXI	CGGCCG	1 cut(s)	161
Eco130I	CCWWGG	1 cut(s)	729
Eco24I	GRGCYC	1 cut(s)	45
Eco47I	GGWCC	1 cut(s)	882
Eco52I	CGGCCG	1 cut(s)	161
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	2 cut(s)	498, 888
Eco88I	CYCGRG	1 cut(s)	888
EcoICRI	GAGCTC	1 cut(s)	43
EcoO109I	RGGNCCY	1 cut(s)	882
EcoT14I	CCWWGG	1 cut(s)	729
EcoT38I	GRGCYC	1 cut(s)	45
EgeI	GGCGCC	1 cut(s)	12
EheI	GGCGCC	1 cut(s)	12
ErhI	CCWWGG	1 cut(s)	729
FaeI	CATG	5 cut(s)	441, 506, 550, 914, 983
FaqI	GGGAC	1 cut(s)	307
FatI	CATG	5 cut(s)	437, 502, 546, 910, 979
FauI	CCCGC	2 cut(s)	44, 393
FauNDI	CATATG	1 cut(s)	654
Fnu4HI	GCNGC	2 cut(s)	161, 765
FriOI	GRGCYC	1 cut(s)	45
Fsp4HI	GCNGC	2 cut(s)	161, 765
FspBI	CTAG	2 cut(s)	756, 1007
FspI	TGCGCA	1 cut(s)	603
GlaI	GCGC	2 cut(s)	12, 603
GluI	GCNGC	2 cut(s)	161, 765
HaeII	RGCGCY	1 cut(s)	14
HaeIII	GGCC	1 cut(s)	163
HhaI	GCGC	2 cut(s)	13, 604
Hin1I	GRCGYC	1 cut(s)	11
Hin1II	CATG	5 cut(s)	441, 506, 550, 914, 983
Hin6I	GCGC	2 cut(s)	11, 602
HinP1I	GCGC	2 cut(s)	11, 602
HincII	GTYRAC	2 cut(s)	523, 864
HindII	GTYRAC	2 cut(s)	523, 864
HinfI	GANTC	8 cut(s)	85, 422, 499, 554, 635, 716, 824, 920
HpaI	GTTAAC	1 cut(s)	864
HphI	GGTGA	3 cut(s)	139, 466, 841
Hpy166II	GTNNAC	4 cut(s)	276, 341, 523, 864
Hpy188I	TCNGA	6 cut(s)	95, 106, 319, 474, 498, 1016
Hpy188III	TCNNGA	5 cut(s)	23, 558, 856, 888, 890
Hpy8I	GTNNAC	4 cut(s)	276, 341, 523, 864
Hpy99I	CGWCG	1 cut(s)	10
HpyCH4III	ACNGT	4 cut(s)	208, 280, 820, 961
HpyCH4IV	ACGT	3 cut(s)	77, 194, 525
HpyCH4V	TGCA	6 cut(s)	170, 217, 247, 252, 276, 767
HpyF10VI	GCNNNNNNNGC	2 cut(s)	223, 599
HpyF3I	CTNAG	3 cut(s)	222, 513, 594
HpySE526I	ACGT	3 cut(s)	77, 194, 525
Hsp92I	GRCGYC	1 cut(s)	11
Hsp92II	CATG	5 cut(s)	441, 506, 550, 914, 983
HspAI	GCGC	2 cut(s)	11, 602
KasI	GGCGCC	1 cut(s)	10
KspAI	GTTAAC	1 cut(s)	864
Kzo9I	GATC	1 cut(s)	433
LmnI	GCTCC	1 cut(s)	238
Lsp1109I	GCAGC	1 cut(s)	751
LweI	GCATC	2 cut(s)	589, 590
MaeI	CTAG	2 cut(s)	756, 1007
MaeII	ACGT	3 cut(s)	77, 194, 525
MaeIII	GTNAC	2 cut(s)	208, 955
MalI	GATC	1 cut(s)	435
MboI	GATC	1 cut(s)	433
MboII	GAAGA	5 cut(s)	76, 365, 881, 907, 949
MflI	RGATCY	1 cut(s)	433
MhlI	GDGCHC	4 cut(s)	45, 83, 278, 378
MluCI	AATT	6 cut(s)	47, 608, 743, 775, 914, 943
Mly113I	GGCGCC	1 cut(s)	11
MlyI	GAGTC	4 cut(s)	431, 508, 563, 725
MmeI	TCCRAC	2 cut(s)	129, 722
MseI	TTAA	6 cut(s)	414, 567, 779, 863, 906, 947
Mva1269I	GAATGC	2 cut(s)	172, 1010
MvnI	CGCG	1 cut(s)	39
MwoI	GCNNNNNNNGC	2 cut(s)	223, 599
NarI	GGCGCC	1 cut(s)	11
NdeI	CATATG	1 cut(s)	654
NdeII	GATC	1 cut(s)	433
NlaIII	CATG	5 cut(s)	441, 506, 550, 914, 983
NlaIV	GGNNCC	2 cut(s)	12, 690
NsbI	TGCGCA	1 cut(s)	603
PaeR7I	CTCGAG	1 cut(s)	888
PctI	GAATGC	2 cut(s)	172, 1010
PfeI	GAWTC	4 cut(s)	85, 635, 824, 920
PflMI	CCANNNNNTGG	1 cut(s)	416
PkrI	GCNGC	2 cut(s)	162, 766
PleI	GAGTC	4 cut(s)	430, 507, 562, 724
PluTI	GGCGCC	1 cut(s)	14
PpsI	GAGTC	4 cut(s)	430, 507, 562, 724
Ppu21I	YACGTR	1 cut(s)	78
PpuMI	RGGWCCY	1 cut(s)	882
Psp124BI	GAGCTC	1 cut(s)	45
Psp5II	RGGWCCY	1 cut(s)	882
PspN4I	GGNNCC	2 cut(s)	12, 690
PspPI	GGNCC	1 cut(s)	882
PspPPI	RGGWCCY	1 cut(s)	882
PstI	CTGCAG	1 cut(s)	769
PsuI	RGATCY	1 cut(s)	433
RsaI	GTAC	3 cut(s)	76, 408, 771
RsaNI	GTAC	3 cut(s)	75, 407, 770
SacI	GAGCTC	1 cut(s)	45
SaqAI	TTAA	6 cut(s)	414, 567, 779, 863, 906, 947
SatI	GCNGC	2 cut(s)	161, 765
Sau3AI	GATC	1 cut(s)	433
Sau96I	GGNCC	1 cut(s)	882
ScaI	AGTACT	1 cut(s)	771
SchI	GAGTC	4 cut(s)	431, 508, 563, 725
SduI	GDGCHC	4 cut(s)	45, 83, 278, 378
SfaNI	GCATC	2 cut(s)	589, 590
SfcI	CTRYAG	1 cut(s)	765
SfoI	GGCGCC	1 cut(s)	12
Sfr274I	CTCGAG	1 cut(s)	888
SinI	GGWCC	1 cut(s)	882
SlaI	CTCGAG	1 cut(s)	888
SmlI	CTYRAG	1 cut(s)	888
SmoI	CTYRAG	1 cut(s)	888
Sse9I	AATT	6 cut(s)	47, 608, 743, 775, 914, 943
SsiI	CCGC	3 cut(s)	37, 160, 386
SspDI	GGCGCC	1 cut(s)	10
SspI	AATATT	1 cut(s)	152
SspMI	CTAG	2 cut(s)	756, 1007
SstI	GAGCTC	1 cut(s)	45
StyI	CCWWGG	1 cut(s)	729
TaaI	ACNGT	4 cut(s)	208, 280, 820, 961
TaiI	ACGT	3 cut(s)	80, 197, 528
TaqI	TCGA	5 cut(s)	83, 425, 584, 719, 889
TasI	AATT	6 cut(s)	47, 608, 743, 775, 914, 943
TatI	WGTACW	1 cut(s)	769
TauI	GCSGC	1 cut(s)	163
TfiI	GAWTC	4 cut(s)	85, 635, 824, 920
Tru1I	TTAA	6 cut(s)	414, 567, 779, 863, 906, 947
Tru9I	TTAA	6 cut(s)	414, 567, 779, 863, 906, 947
TscAI	CASTG	3 cut(s)	254, 278, 678
TseI	GCWGC	1 cut(s)	764
TspDTI	ATGAA	4 cut(s)	20, 459, 927, 968
TspGWI	ACGGA	2 cut(s)	752, 1020
TspRI	CASTG	3 cut(s)	254, 278, 678
Van91I	CCANNNNNTGG	1 cut(s)	416
VneI	GTGCAC	1 cut(s)	274
VpaK11BI	GGWCC	1 cut(s)	882
XapI	RAATTY	2 cut(s)	47, 775
XhoI	CTCGAG	1 cut(s)	888
XspI	CTAG	2 cut(s)	756, 1007
ZrmI	AGTACT	1 cut(s)	771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.