Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005752

Physical Location & Seq

Genomic Coordinates Forward (+)

14438965 .. 14443567

Gene Structure 4603 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1174800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2913 bp

ATGGATTGCAATAAGGAAGAGGCCATTAGGGCCAAGGTGATTGCTGAGAAGAAGATGCAGAGCAAGGATTTCGTGGGGGCTCGTAAGATTGCTATAAAGGCGCAACAACTCTATCCTGATCTGGAGAATGCATCTCAGATGCTTATGGTTTGTGATGTGCATTGCTCTGCTGAAAAATTGTTTGGGACTGAGATGGACTGGTATGGTATCCTTCAGATTGATCAAACAGCGGATGAGATAACAATTAAGAAGCAGTACAGGAAGTTTGCTCTCCAACTTCATCCTGATAAAAACAAGTTTTCTGGTGCAGAAGCTGCTTTTAAGCTGATTGGTGAAGCTCAAAGAGTGCTTTTGGACCGTGACAAACGGAGCATGCATGACCTGAAACGCAAGACTTCTGTGAGTAAAACAGGTGTGCCACATCGACCCCCACATAGGGCGAGTTGGAACTCAAATCCTGGAGTTCAAAACAACTCCAGAGGCAACTTTACAGGCGTTAATCTCCAGAGCCAGCAGCAACAACAGCCTTTTCCACCGGGGTATTCAGATGCCCGATCGACTTTTTGGACTGCCTGCCCATTTTGTTCAGTTAAGTACCAGTATTATAGAGAAGTTCTTCTTAGATCTCTTCGTTGTCAAGGCTGCGGTAAGCCCTTCATTGCATATGACACAAATGTGCCAGGTGGCGCACCACCACCACCACCACCACCACCACCAACTAACTTAAGTCAGCAGGCATTCCCCCCCAAAAAAGTCAATGTCAACATGTCTGAAGTGAGACTTCAGAGGAATGTTGGAGCTGAAAACTCCAAAGCTAAACCTTTCCAAAAGACAGACCAAAGGTCAAGTAGCTCTTCAGGTGTTCGTTCAGAAAAGGTAAAGCGTAAGAGAGACAGGAAACGTGTAGTAGAATCCAGTGAAAGCAGTGACTCTGAAAGCAACAGTGATTCTGAAGAAGATATGGTCATTGATGATGCAAGCGGCAGTGATTCTGAAGAAGATATGGTCATTGATGATGCTGTTCTTCAGGCTGGACTGAAATCTGGAATTTATGGAAACCAGCAACCACGGAGATCTTCACGGCATAAGCAGCATATTTCTTATAAGGAAAATCTAAGCGATGGTGAAGACACTCCTCTATCAAAAAGGGAGAAGAGGACTGTCTCATCCTGTGCCACTGAAGAGGAAAATGAAGACGCATCTAAAGTGGAGGAATCTATAATGAATAATAAATCTGATCGTGCCGCTGACACTAAAGGAGATGAAAAGGTGAAACAGAAAGAAGAGTGTTTGACAAATAGAGTTGGGGAAAAAAAGTCTGGGGCTAAAGAAAGGGTGAAAGTATTTGACTCTAAGAAAATTTCTGAGGCACTTGAGAATTCTGCATCACATAAAAGCTCCAATGAAAAACAAGAACCAGACCCCTTGTATTCTTTTCCTGAGAGTGATTTCAACGACTTTGAGAGGTACCGGAAAGAAGAACTCTTTGAAGCTGGGCAGGTGTGGGCTATTTATGATACTCGAAATGGGATGCCAAGATTCTATGCTAGAATCAAGAAAGTTCACTCACCTGAGTTCAAGTTGCAGATAACCTGGCTAGAGCCTGACCCCGATGATGATAATGAAATGAAATGGGCCAAATCTGACTTGCCGTTTTCTTGTGGTAAATTCAGACAAGGTGACTCTGAAATCACGAAAGATCTTCCCATGTTCTCTCATTTGATGACTTGGGGGAAGATTAGAAGTTCTTACATGATATATCCAAGAAAGGGAGAGATTTGGGCCATTTTCAAGAACTGGGACATAAACTTGTACACTGATGCAGACTCTAATCAGAAGCTGAACTTTGAATACGAATTTGTTGATATTTTATCAGACTATTCTGAGGATGTTGGAATATCTGTTGCACTCTTGGACAAGGTGAAAGGTTTTGTGAGTGTTTTCTGTCGAAGGGTTAAGGATGGGAAAGGCACATTTCAAGTTCCACCAGGAGAATTATTGAGGTTTTCCCACAGAGTTCCATCTTATACATTGACTGGTGACGAAGGAGTAGGAATTCCATCAGGATCTGTTGAGCTTGATCCCGCCTCTTTGCCTTTTAATATTGAAGAAGTTCCTATTTCTAGAGATCGGAAAACGGAGGCTAGTACTCATAGTAATGGTTTTTCTAAATCTCCGGATACAAGGAATGAAGACAGTAATGTTCCCAAAGGGTCATCGCCAGAAGGCATCGCTATTCCAGAAGCTGAGTTCTACAACTTTGATGCTGATAAATCTCTGGAAAAGTTTGAGATTGGTCAGATCTGGGCATTGTATAGTGATGAGGATGGCTTGCCAAAATACTATGGCCAGATAAAAAACCTTGATTGTCACCGATCCAAATTGCATATTGCATGGCTTGCTTCCAACTTACTACCAGACAGGGTGATTCGATGGCATGATGAGGGGATGCCTGTTTGCTGCGGGAGATTTAGGGTTAGAAGGAGTACGCTCCAGGACTATGATTCTACTCTGTCCTTTTCACACCGGGTAAATGCAATTTCTATTGGTAAGAATGATTTTGAAATATATCCTCAAAGAGGTGAGGTTTGGGCGTTGTATAAGAACTGGACAGCTGATCTCTCGTGTTCTGACTTGGATACCTGCGAGTATGACATAGTGGCAGTGTGGAAAGAAAATGATATTCAAAGAGAGGTCTTGATTTTAGAGCGTGTGGATGGTTTCAACTCAGTTTTCAAGACTCAGTTGAAAGGAGGATCAGCTGAAACCATGACCATCCGTGGGGTTGAGCTACTAAGGTTTTCTCACATGATTCCCTCTTTCAAGCTAACAGACCAAAAAGGCGGCAGCCTCAGAGGGTGCTGGGAACTTGATCCTGCTGCATTGCCGCTTCGGTTCTTTAGTCAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006457 GO:0006458 GO:0006508 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019538 GO:0022607 GO:0030163 GO:0030176 GO:0030544 GO:0031072 GO:0031224 GO:0031227 GO:0031984 GO:0033554 GO:0034622 GO:0034976 GO:0035966 GO:0035967 GO:0036503 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051084 GO:0051085 GO:0051603 GO:0051716 GO:0051788 GO:0061077 GO:0065003 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071840 GO:0098827 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3444 DnaJ

Protein Analysis

971

Amino Acids

110.12

Weight (kDa)

6.2

Isoelectric Point (pI)

49.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	66 - 127	4.6e-19	DnaJ domain
DUF3444	PF11926	477 - 682	1.3e-78	Domain of unknown function (DUF3444)
DUF3444	PF11926	742 - 946	1.4e-63	Domain of unknown function (DUF3444)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005752)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06340 AT3G06340 AT3G06340 AT3G06340 AT5G18750
fragaria_vesca	FvH4_2g06370
malus_domestica	MD13G1174800.v1.1 MD16G1176500.v1.1
prunus_persica	Prupe.1G143400_v2.0.a1 Prupe.1G143400_v2.0.a1 Prupe.1G143400_v2.0.a1 Prupe.1G143400_v2.0.a1
pyrus_communis	pycom13g15060 pycom13g15070 pycom16g14800 pycom16g14810
rosa_chinensis	RchiOBHm_Chr4g0420721
rosa_laevigata	RLG00000007740 RLG00000030269 RLG00000030290
rosa_multiflora	Rmu_co8498373.1_g000001
rosa_roxburghii	Rroxscaffold_5G00363230
rosa_rugosa	Rorug04G0165700
rosa_samantha	Rh4AG227200 Rh4BG230000 Rh4CG242100 Rh4DG225500
rosa_wichuraiana	Rw4G019660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1104
AarI	CACCTGC	1 cut(s)	1489
Acc36I	ACCTGC	2 cut(s)	1489, 2651
Acc65I	GGTACC	1 cut(s)	1467
AccB1I	GGYRCC	1 cut(s)	1467
AccIII	TCCGGA	1 cut(s)	2176
AciI	CCGC	8 cut(s)	230, 645, 981, 1245, 2085, 2463, 2844, 2888
AclWI	GGATC	5 cut(s)	2074, 2075, 2370, 2764, 2867
AcoI	YGGCCR	1 cut(s)	2347
AcsI	RAATTY	6 cut(s)	1047, 1359, 1378, 1667, 1856, 2055
AcuI	CTGAAG	8 cut(s)	197, 767, 792, 840, 972, 1010, 1014, 1200
AfaI	GTAC	6 cut(s)	257, 596, 1469, 1814, 2149, 2488
AfiI	CCNNNNNNNGG	5 cut(s)	435, 436, 1769, 2579, 2781
AflII	CTTAAG	1 cut(s)	724
AflIII	ACRYGT	2 cut(s)	765, 901
AhdI	GACNNNNNGTC	1 cut(s)	841
AjnI	CCWGG	5 cut(s)	457, 679, 1592, 1987, 2493
AjuI	GAANNNNNNNTTGG	2 cut(s)	165, 197
AleI	CACNNNNGTG	1 cut(s)	674
Alw26I	GTCTC	3 cut(s)	772, 885, 1168
AlwI	GGATC	5 cut(s)	2074, 2075, 2370, 2764, 2867
AlwNI	CAGNNNCTG	4 cut(s)	314, 1840, 2069, 2246
Aor13HI	TCCGGA	1 cut(s)	2176
AoxI	GGCC	5 cut(s)	21, 30, 1635, 1782, 2347
ApeKI	GCWGC	7 cut(s)	314, 514, 642, 1090, 2460, 2847, 2879
ApoI	RAATTY	6 cut(s)	1047, 1359, 1378, 1667, 1856, 2055
Asp700I	GAANNNNTTC	2 cut(s)	615, 2112
Asp718I	GGTACC	1 cut(s)	1467
AspLEI	GCGC	2 cut(s)	103, 689
AspS9I	GGNCC	4 cut(s)	30, 355, 1635, 1782
AsuC2I	CCSGG	2 cut(s)	537, 2528
AvaII	GGWCC	1 cut(s)	355
BalI	TGGCCA	1 cut(s)	2349
BanI	GGYRCC	1 cut(s)	1467
BanII	GRGCYC	1 cut(s)	82
BauI	CACGAG	1 cut(s)	2623
BbsI	GAAGAC	3 cut(s)	1134, 1200, 2199
BbvI	GCAGC	7 cut(s)	301, 526, 629, 1102, 2447, 2859, 2866
BccI	CCATC	9 cut(s)	187, 1115, 1955, 2029, 2068, 2321, 2427, 2711, 2783
BceAI	ACGGC	2 cut(s)	1097, 1636
BcgI	CGANNNNNNTGC	2 cut(s)	52, 86
BciT130I	CCWGG	5 cut(s)	459, 681, 1594, 1989, 2495
BciVI	GTATCC	3 cut(s)	218, 2173, 2632
BclI	TGATCA	1 cut(s)	220
BcnI	CCSGG	2 cut(s)	537, 2528
BcoDI	GTCTC	3 cut(s)	772, 885, 1168
BfaI	CTAG	4 cut(s)	1548, 1598, 2124, 2145
BfrI	CTTAAG	1 cut(s)	724
BfuAI	ACCTGC	2 cut(s)	1489, 2651
BfuI	GTATCC	3 cut(s)	218, 2173, 2632
BglI	GCCNNNNNGGC	1 cut(s)	29
BglII	AGATCT	4 cut(s)	623, 1073, 1699, 2301
BmcAI	AGTACT	1 cut(s)	2149
Bme1390I	CCNGG	7 cut(s)	459, 537, 681, 1594, 1989, 2495, 2528
Bme18I	GGWCC	1 cut(s)	355
BmeRI	GACNNNNNGTC	1 cut(s)	841
BmgT120I	GGNCC	4 cut(s)	30, 355, 1635, 1782
BmiI	GGNNCC	1 cut(s)	1469
BmrFI	CCNGG	7 cut(s)	459, 537, 681, 1594, 1989, 2495, 2528
BmrI	ACTGGG	1 cut(s)	1807
BmuI	ACTGGG	1 cut(s)	1807
BpiI	GAAGAC	3 cut(s)	1134, 1200, 2199
BpmI	CTGGAG	5 cut(s)	143, 460, 480, 488, 2477
BpuEI	CTTGAG	1 cut(s)	1394
BpuMI	CCSGG	2 cut(s)	537, 2528
BsaJI	CCNNGG	4 cut(s)	33, 536, 1067, 2779
BsaWI	WCCGGW	2 cut(s)	1470, 2176
Bsc4I	CCNNNNNNNGG	5 cut(s)	435, 436, 1769, 2579, 2781
Bse1I	ACTGG	6 cut(s)	203, 598, 915, 1802, 2041, 2612
Bse3DI	GCAATG	3 cut(s)	160, 657, 2882
BseAI	TCCGGA	1 cut(s)	2176
BseBI	CCWGG	5 cut(s)	459, 681, 1594, 1989, 2495
BseDI	CCNNGG	4 cut(s)	33, 536, 1067, 2779
BseLI	CCNNNNNNNGG	5 cut(s)	435, 436, 1769, 2579, 2781
BseMI	GCAATG	3 cut(s)	160, 657, 2882
BseNI	ACTGG	6 cut(s)	203, 598, 915, 1802, 2041, 2612
BseRI	GAGGAG	1 cut(s)	1125
BseXI	GCAGC	7 cut(s)	301, 526, 629, 1102, 2447, 2859, 2866
BseYI	CCCAGC	2 cut(s)	1493, 2862
BsgI	GTGCAG	1 cut(s)	327
Bsh1285I	CGRYCG	1 cut(s)	557
BshFI	GGCC	5 cut(s)	23, 32, 1637, 1784, 2349
BshNI	GGYRCC	1 cut(s)	1467
BsiEI	CGRYCG	1 cut(s)	557
BsiSI	CCGG	4 cut(s)	536, 1471, 2177, 2527
BslFI	GGGAC	2 cut(s)	199, 1814
BslI	CCNNNNNNNGG	5 cut(s)	435, 436, 1769, 2579, 2781
BsmAI	GTCTC	3 cut(s)	772, 885, 1168
BsmFI	GGGAC	2 cut(s)	199, 1814
BsmI	GAATGC	2 cut(s)	133, 737
BsnI	GGCC	5 cut(s)	23, 32, 1637, 1784, 2349
Bsp1286I	GDGCHC	1 cut(s)	82
Bsp13I	TCCGGA	1 cut(s)	2176
Bsp1407I	TGTACA	1 cut(s)	1812
BspACI	CCGC	8 cut(s)	230, 645, 981, 1245, 2085, 2463, 2844, 2888
BspANI	GGCC	5 cut(s)	23, 32, 1637, 1784, 2349
BspEI	TCCGGA	1 cut(s)	2176
BspLI	GGNNCC	1 cut(s)	1469
BspMI	ACCTGC	2 cut(s)	1489, 2651
BspPI	GGATC	5 cut(s)	2074, 2075, 2370, 2764, 2867
BspQI	GCTCTTC	1 cut(s)	859
BspT107I	GGYRCC	1 cut(s)	1467
BspTI	CTTAAG	1 cut(s)	724
BsrDI	GCAATG	3 cut(s)	160, 657, 2882
BsrGI	TGTACA	1 cut(s)	1812
BsrI	ACTGG	6 cut(s)	203, 598, 915, 1802, 2041, 2612
BssECI	CCNNGG	4 cut(s)	33, 536, 1067, 2779
BssSI	CACGAG	1 cut(s)	2623
BssT1I	CCWWGG	1 cut(s)	33
Bst2BI	CACGAG	1 cut(s)	2623
Bst2UI	CCWGG	5 cut(s)	459, 681, 1594, 1989, 2495
Bst4CI	ACNGT	4 cut(s)	359, 944, 1162, 2198
Bst6I	CTCTTC	6 cut(s)	12, 633, 859, 1148, 1176, 1278
BstAFI	CTTAAG	1 cut(s)	724
BstAPI	GCANNNNNTGC	2 cut(s)	314, 2399
BstAUI	TGTACA	1 cut(s)	1812
BstC8I	GCNNGC	7 cut(s)	374, 512, 574, 735, 979, 2333, 2400
BstDSI	CCRYGG	2 cut(s)	1067, 2779
BstENI	CCTNNNNNAGG	1 cut(s)	2577
BstHHI	GCGC	2 cut(s)	103, 689
BstMAI	GTCTC	3 cut(s)	772, 885, 1168
BstMCI	CGRYCG	1 cut(s)	557
BstMWI	GCNNNNNNNGC	6 cut(s)	29, 98, 314, 523, 1090, 2399
BstNI	CCWGG	5 cut(s)	459, 681, 1594, 1989, 2495
BstNSI	RCATGY	2 cut(s)	376, 769
BstSCI	CCNGG	7 cut(s)	457, 535, 679, 1592, 1987, 2493, 2526
BstV1I	GCAGC	7 cut(s)	301, 526, 629, 1102, 2447, 2859, 2866
BstV2I	GAAGAC	3 cut(s)	1134, 1200, 2199
BstX2I	RGATCY	5 cut(s)	623, 1073, 1699, 2066, 2301
BstYI	RGATCY	5 cut(s)	623, 1073, 1699, 2066, 2301
BsuI	GTATCC	3 cut(s)	218, 2173, 2632
BsuRI	GGCC	5 cut(s)	23, 32, 1637, 1784, 2349
BtgI	CCRYGG	2 cut(s)	1067, 2779
BtgZI	GCGATG	3 cut(s)	1134, 2202, 2215
BtsI	GCAGTG	3 cut(s)	931, 991, 2670
BtsIMutI	CAGTG	7 cut(s)	922, 931, 949, 991, 1176, 1815, 2670
BveI	ACCTGC	2 cut(s)	1489, 2651
Cac8I	GCNNGC	7 cut(s)	374, 512, 574, 735, 979, 2333, 2400
CaiI	CAGNNNCTG	4 cut(s)	314, 1840, 2069, 2246
CfoI	GCGC	2 cut(s)	103, 689
Cfr13I	GGNCC	4 cut(s)	30, 355, 1635, 1782
CseI	GACGC	1 cut(s)	1205
Csp6I	GTAC	6 cut(s)	256, 595, 1468, 1813, 2148, 2487
CviAII	CATG	9 cut(s)	373, 377, 766, 1708, 1753, 2394, 2438, 2770, 2809
CviQI	GTAC	6 cut(s)	256, 595, 1468, 1813, 2148, 2487
DriI	GACNNNNNGTC	1 cut(s)	841
EaeI	YGGCCR	1 cut(s)	2347
Eam1104I	CTCTTC	6 cut(s)	12, 633, 859, 1148, 1176, 1278
Eam1105I	GACNNNNNGTC	1 cut(s)	841
EarI	CTCTTC	6 cut(s)	12, 633, 859, 1148, 1176, 1278
Eco130I	CCWWGG	1 cut(s)	33
Eco24I	GRGCYC	1 cut(s)	82
Eco47I	GGWCC	1 cut(s)	355
Eco57I	CTGAAG	8 cut(s)	197, 767, 792, 840, 972, 1010, 1014, 1200
EcoNI	CCTNNNNNAGG	1 cut(s)	2577
EcoRI	GAATTC	2 cut(s)	1378, 2055
EcoRII	CCWGG	5 cut(s)	457, 679, 1592, 1987, 2493
EcoT14I	CCWWGG	1 cut(s)	33
EcoT22I	ATGCAT	2 cut(s)	133, 378
EcoT38I	GRGCYC	1 cut(s)	82
ErhI	CCWWGG	1 cut(s)	33
FaeI	CATG	9 cut(s)	376, 380, 769, 1711, 1756, 2397, 2441, 2773, 2812
FalI	AAGNNNNNCTT	8 cut(s)	603, 635, 765, 797, 838, 870, 1829, 1861
FaqI	GGGAC	2 cut(s)	199, 1814
FatI	CATG	9 cut(s)	372, 376, 765, 1707, 1752, 2393, 2437, 2769, 2808
FauI	CCCGC	2 cut(s)	2092, 2456
FauNDI	CATATG	1 cut(s)	664
FbaI	TGATCA	1 cut(s)	220
FriOI	GRGCYC	1 cut(s)	82
FspBI	CTAG	4 cut(s)	1548, 1598, 2124, 2145
GlaI	GCGC	2 cut(s)	102, 688
GsaI	CCCAGC	2 cut(s)	1497, 2866
GsuI	CTGGAG	5 cut(s)	143, 460, 480, 488, 2477
HaeIII	GGCC	5 cut(s)	23, 32, 1637, 1784, 2349
HapII	CCGG	4 cut(s)	536, 1471, 2177, 2527
HgaI	GACGC	1 cut(s)	1205
HhaI	GCGC	2 cut(s)	103, 689
Hin1II	CATG	9 cut(s)	376, 380, 769, 1711, 1756, 2397, 2441, 2773, 2812
Hin6I	GCGC	2 cut(s)	101, 687
HinP1I	GCGC	2 cut(s)	101, 687
HincII	GTYRAC	1 cut(s)	763
HindII	GTYRAC	1 cut(s)	763
HpaII	CCGG	4 cut(s)	536, 1471, 2177, 2527
Hpy166II	GTNNAC	3 cut(s)	763, 1564, 1815
Hpy8I	GTNNAC	3 cut(s)	763, 1564, 1815
HpyAV	CCTTC	6 cut(s)	221, 664, 1944, 2039, 2219, 2475
HpyCH4III	ACNGT	4 cut(s)	359, 944, 1162, 2198
HpyCH4IV	ACGT	1 cut(s)	901
HpyF10VI	GCNNNNNNNGC	6 cut(s)	29, 98, 314, 523, 1090, 2399
HpySE526I	ACGT	1 cut(s)	901
Hsp92II	CATG	9 cut(s)	376, 380, 769, 1711, 1756, 2397, 2441, 2773, 2812
HspAI	GCGC	2 cut(s)	101, 687
Kpn2I	TCCGGA	1 cut(s)	2176
KpnI	GGTACC	1 cut(s)	1471
Ksp22I	TGATCA	1 cut(s)	220
LguI	GCTCTTC	1 cut(s)	859
LmnI	GCTCC	4 cut(s)	369, 797, 1403, 2496
Lsp1109I	GCAGC	7 cut(s)	301, 526, 629, 1102, 2447, 2859, 2866
MaeI	CTAG	4 cut(s)	1548, 1598, 2124, 2145
MaeII	ACGT	1 cut(s)	901
MaeIII	GTNAC	5 cut(s)	359, 926, 1679, 2039, 2369
MflI	RGATCY	5 cut(s)	623, 1073, 1699, 2066, 2301
MhlI	GDGCHC	1 cut(s)	82
MlsI	TGGCCA	1 cut(s)	2349
MluNI	TGGCCA	1 cut(s)	2349
MlyI	GAGTC	5 cut(s)	923, 1343, 1676, 1820, 2734
MmeI	TCCRAC	5 cut(s)	298, 425, 775, 1873, 2430
Mox20I	TGGCCA	1 cut(s)	2349
Mph1103I	ATGCAT	2 cut(s)	133, 378
MroI	TCCGGA	1 cut(s)	2176
MroXI	GAANNNNTTC	2 cut(s)	615, 2112
MscI	TGGCCA	1 cut(s)	2349
MseI	TTAA	7 cut(s)	246, 321, 498, 591, 725, 1956, 2100
MslI	CAYNNNNRTG	2 cut(s)	674, 2157
Msp20I	TGGCCA	1 cut(s)	2349
MspA1I	CMGCKG	4 cut(s)	230, 1247, 2615, 2762
MspCI	CTTAAG	1 cut(s)	724
MspI	CCGG	4 cut(s)	536, 1471, 2177, 2527
MspR9I	CCNGG	7 cut(s)	459, 537, 681, 1594, 1989, 2495, 2528
Mva1269I	GAATGC	2 cut(s)	133, 737
MvaI	CCWGG	5 cut(s)	459, 681, 1594, 1989, 2495
MwoI	GCNNNNNNNGC	6 cut(s)	29, 98, 314, 523, 1090, 2399
NciI	CCSGG	2 cut(s)	537, 2528
NdeI	CATATG	1 cut(s)	664
NlaIII	CATG	9 cut(s)	376, 380, 769, 1711, 1756, 2397, 2441, 2773, 2812
NlaIV	GGNNCC	1 cut(s)	1469
NmuCI	GTSAC	5 cut(s)	359, 926, 1679, 2039, 2369
NsiI	ATGCAT	2 cut(s)	133, 378
NspI	RCATGY	2 cut(s)	376, 769
OliI	CACNNNNGTG	1 cut(s)	674
PaeI	GCATGC	1 cut(s)	376
PaqCI	CACCTGC	1 cut(s)	1489
PciI	ACATGT	1 cut(s)	765
PciSI	GCTCTTC	1 cut(s)	859
PctI	GAATGC	2 cut(s)	133, 737
PdmI	GAANNNNTTC	2 cut(s)	615, 2112
PfeI	GAWTC	9 cut(s)	911, 947, 989, 1214, 1539, 1551, 2428, 2504, 2812
PfoI	TCCNGGA	2 cut(s)	457, 2493
Ple19I	CGATCG	1 cut(s)	557
PleI	GAGTC	5 cut(s)	923, 1343, 1676, 1820, 2734
PpsI	GAGTC	5 cut(s)	923, 1343, 1676, 1820, 2734
PscI	ACATGT	1 cut(s)	765
PsiI	TTATAA	1 cut(s)	1104
Psp6I	CCWGG	5 cut(s)	457, 679, 1592, 1987, 2493
PspFI	CCCAGC	2 cut(s)	1493, 2862
PspGI	CCWGG	5 cut(s)	457, 679, 1592, 1987, 2493
PspN4I	GGNNCC	1 cut(s)	1469
PspPI	GGNCC	4 cut(s)	30, 355, 1635, 1782
PsrI	GAACNNNNNNTAC	2 cut(s)	1835, 1867
PstNI	CAGNNNCTG	4 cut(s)	314, 1840, 2069, 2246
PsuI	RGATCY	5 cut(s)	623, 1073, 1699, 2066, 2301
PvuI	CGATCG	1 cut(s)	557
PvuII	CAGCTG	2 cut(s)	2615, 2762
RsaI	GTAC	6 cut(s)	257, 596, 1469, 1814, 2149, 2488
RsaNI	GTAC	6 cut(s)	256, 595, 1468, 1813, 2148, 2487
RseI	CAYNNNNRTG	2 cut(s)	674, 2157
SapI	GCTCTTC	1 cut(s)	859
SaqAI	TTAA	7 cut(s)	246, 321, 498, 591, 725, 1956, 2100
Sau96I	GGNCC	4 cut(s)	30, 355, 1635, 1782
ScaI	AGTACT	1 cut(s)	2149
SchI	GAGTC	5 cut(s)	923, 1343, 1676, 1820, 2734
ScrFI	CCNGG	7 cut(s)	459, 537, 681, 1594, 1989, 2495, 2528
SduI	GDGCHC	1 cut(s)	82
SfiI	GGCCNNNNNGGCC	1 cut(s)	29
SinI	GGWCC	1 cut(s)	355
SmiMI	CAYNNNNRTG	2 cut(s)	674, 2157
SmlI	CTYRAG	2 cut(s)	724, 1373
SmoI	CTYRAG	2 cut(s)	724, 1373
SphI	GCATGC	1 cut(s)	376
SsiI	CCGC	8 cut(s)	230, 645, 981, 1245, 2085, 2463, 2844, 2888
SspI	AATATT	1 cut(s)	2104
SspMI	CTAG	4 cut(s)	1548, 1598, 2124, 2145
StyD4I	CCNGG	7 cut(s)	457, 535, 679, 1592, 1987, 2493, 2526
StyI	CCWWGG	1 cut(s)	33
TaaI	ACNGT	4 cut(s)	359, 944, 1162, 2198
TaiI	ACGT	1 cut(s)	904
TaqI	TCGA	5 cut(s)	424, 557, 1522, 1948, 2431
TatI	WGTACW	3 cut(s)	255, 1812, 2147
TauI	GCSGC	4 cut(s)	984, 1247, 2847, 2890
TfiI	GAWTC	9 cut(s)	911, 947, 989, 1214, 1539, 1551, 2428, 2504, 2812
Tru1I	TTAA	7 cut(s)	246, 321, 498, 591, 725, 1956, 2100
Tru9I	TTAA	7 cut(s)	246, 321, 498, 591, 725, 1956, 2100
TscAI	CASTG	7 cut(s)	922, 931, 949, 991, 1183, 1822, 2670
TseFI	GTSAC	5 cut(s)	359, 926, 1679, 2039, 2369
TseI	GCWGC	7 cut(s)	314, 514, 642, 1090, 2460, 2847, 2879
Tsp45I	GTSAC	5 cut(s)	359, 926, 1679, 2039, 2369
TspDTI	ATGAA	9 cut(s)	269, 646, 1206, 1238, 1278, 1419, 1638, 1643, 2205
TspGWI	ACGGA	4 cut(s)	382, 1084, 2153, 2768
TspRI	CASTG	7 cut(s)	922, 931, 949, 991, 1183, 1822, 2670
Vha464I	CTTAAG	1 cut(s)	724
VpaK11BI	GGWCC	1 cut(s)	355
XagI	CCTNNNNNAGG	1 cut(s)	2577
XapI	RAATTY	6 cut(s)	1047, 1359, 1378, 1667, 1856, 2055
XbaI	TCTAGA	1 cut(s)	2123
XceI	RCATGY	2 cut(s)	376, 769
XmnI	GAANNNNTTC	2 cut(s)	615, 2112
XspI	CTAG	4 cut(s)	1548, 1598, 2124, 2145
ZrmI	AGTACT	1 cut(s)	2149
Zsp2I	ATGCAT	2 cut(s)	133, 378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.