Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005752

Physical Location & Seq

Genomic Coordinates Forward (+)

15032674 .. 15036211

Gene Structure 3538 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1176500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2865 bp

ATGGATTGCAATAAGGAGGAGGCCATTAGGGCCAAGTCGATTGCTGAGAAGAAGATGCAGAGCAAGGATTTCGTGGGGGCTCGTAAGATTGCTATAAAGGCGCAACAACTCTATTCTGATCTGGAGAATATATCTCAGATGCTTATGGTTTGTGATGTGCATTGCTCTGCTGAGAAATTATTTGGGACTGAGATGGACTGGTATGGCATCCTTCAGATTGATCAAACAGCGGATGAGATAACAATTAAGAAGCAGTACAGGAAGTTTGCTCTCCAACTTCATCCTGATAAAAATAAGTTTTCCGGTGCAGAAGCTGCTTTTAAGCTGATAGGTGAAGCTCAAAGAGTACTTTTGGACCGTGACAAACGGAGCATGCATGACCTGAAACGCAAGACTTTTGTGAGTAAAACAGGTGTACCACATCAGCCCCCGCAGAGGGCGAGTTGGAACTCAAATCCTGGAGTTCAAAACAACTCCAGAGGCAACTTTTCAGGCGTTAATCTCCAGAGCCAACAGCAACAGCAGCCTTTTCCACCAGGGTATTCAGATGCCCGATCCACTTTTTGGACTGCCTGCCCATTTTGTTCAGTTAAGTACCAGTATTATAGAGAAGTTCTTCTTAGATCTCTTCGTTGTCAAGGCTGCGGTAAGCCCTTCATTGCATATGACACAAATGTGCCAGGTGGAGCACCACCACCACCACCACCAACTAACTTAAGTCAGCAGGCATTCCCCCCCAAAAAAGTGAATGTTAACATGTCTGAAGTGACATTTCAGAGGAATGTTGGAGCTGAGAACTCCAAAGCGAAACCTTTCCAAAAGACAGAGAAAAAGGCAAGTAGCTCTTCAGGTGTCCGCCCCGAAAAGGTAAAGCGTAAGAGAGAAAGGAAACGTGTAGTAGAATCCAGTGAAAGCAGTGTCTCTGAAAGCAGCAGTGATTCTGAAGAAGATATGGTCATCAATGATGCTGTTCTTCAGGCTGGTTTGAAATCTGGAATTTATGGAGAGCAGCAACCACGGAGGTCTTCACGGCATAAGCAGAACGTTTCTTATAAGGAACATCTAAGTGATGGTGAAGACACTCCTTTATCAAAAAGGGAGAAGAGGAGTGGTTCATCCTGTGCCACTGAAGAGGAAGTTGAAGACGCATTAAAAGAGGAGGAATCTAACATGAATAATAAATCTGATCGTGCTGCTGACGCTACAGGAGATGAGGAAAAGGTGAAACAGAAAGAAAGTGCTTATTTTGAAGAGTGTTTGCCAAAGGGAGTTGGTGAAAAGAAGTTTGGGGCTAAAGAAAGGGTGAAAGTATTCGATTCGAAGAAAAGAAGTCTTGAGAATTCTCCATTAGATAAAAGCTCCAATGAGAAACAAGAACCAGACCCCTTGTATTCTTTCCCTGATAGTGATTTCAACGACTTTGAGAGGTACCGGAAAGAAGAACTCTTTGAAGCTGGGCAGGTGTGGGCTATTTATGATACTCGAAATGGGATGCCAAGATTCTATGCTAGAATCAAGAAAGTTCACTCACCTGAGTTCAAGTTGCAGATAACCTGGCTAGAGCCGGACCCTGATGATGATAATGAAAAGAAATGGGCCAAAGCTGACTTGCCATTTTCTTGTGGTAAATTGAGACAAGGTCACTCTGAAACCACGAAAGATCTTCCCATGTTCTCTCATTTGATGATTTGGGGGAAGATTAGAAGTGCTTACATGATATATCCAAGAAGGGGAGAGACTTGGGCCATTTTCAAGAACTGGGATATAAAGTGGTTCTCTGATCCGGACTCCAATCAGAAGCACTTTGAATATGAATTTGTTGATATTTTGTCAGACTATTCTAAGGATGTTGGAATATATGTTGCACTCTTGGAGAAGGTGAAAAGCTTTGTGAGTGTTTTTCGAAGGGTTAAGGATGGGAAAGGCACATTTCAAGTTCCATCCGGAGAATTATTGAGGTTTTCCCACAGAGTTCCATCTTATACATTGACTGGTGACGAAGGAGTAGGAGTTCCATTAGGATCTGTTGAGCTTGATCCCGCCGCTTTGCCTTTTAATATTGAAGAAGTTCCTGTTTCTAGAGAACGGAAAACGGAGGCTAGTACTCGTACTAATGGTTTTTCTGCATCTCCGGATACAAGGAATGAAGATGGTAATGTTCCCAAAGGATCATCCCCAGAAGGTATCGATATTCCGGAAGCTGAGTTCTACAACTTTGATGCTGATAAATCTCTGGAAAAGTTTGAGATTGGTCAGATCTGGGCATTGTATAGTGATGAGGATGGCTTGCCAAAATACTATGGCCAGATAAAGAGCATTGATAGTCACCGATCCAAATTGCATATTGCATGGCTTGCCAACTTACTACCAGACAATGTGATCCGATGGCATGATGAGGAGATGCCCGTCAGCTGCGGGAGATTTAGGGTTAGAAGGGGTCCACTCCAGGACTATGATTCTACTCTGTCCTTTTCACACCGGTTAAATGCAGTTTCTATTGGTAAGAATGATTTTGAAATATATCCACACAGAGGTGAGGTTTGGGCTTTATATAAGAACTGGACAGCTCATTTCTCGTGTTCCGACTTGGATACCTGCGAGTATGACATTGTGGTGGTGCAGACTGAAAATGATTTGCAAAGAGAGGTCTTGATTTTAGAGCGTGTGGATGGTTTCAACTCGGTTTTCAAGACTCGGGCGAAGGGAGGATCAGCTGAAACCATGACCGTCCGTGGGGTTGAGCTACTGAGGTTTTCTCACATGATTCCTTCTTTCCAGCTGACGGACGAAAAAGGCGGCAGCCTCAGAGGATGCTGGGAACTTGACCCGGCTGCTTTGCCGCTTCGTTTCTTCAGTCAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006457 GO:0006458 GO:0006508 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0019538 GO:0022607 GO:0030163 GO:0030176 GO:0030544 GO:0031072 GO:0031224 GO:0031227 GO:0031984 GO:0033554 GO:0034622 GO:0034976 GO:0035966 GO:0035967 GO:0036503 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051084 GO:0051085 GO:0051603 GO:0051716 GO:0051788 GO:0061077 GO:0065003 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071840 GO:0098827 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3444 DnaJ

Protein Analysis

955

Amino Acids

108.6

Weight (kDa)

6.58

Isoelectric Point (pI)

51.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	66 - 127	4.5e-19	DnaJ domain
DUF3444	PF11926	464 - 667	1.2e-75	Domain of unknown function (DUF3444)
DUF3444	PF11926	727 - 930	9.9e-62	Domain of unknown function (DUF3444)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005752)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06340 AT3G06340 AT3G06340 AT3G06340 AT5G18750
fragaria_vesca	FvH4_2g06370
malus_domestica	MD13G1174800.v1.1 MD16G1176500.v1.1
prunus_persica	Prupe.1G143400_v2.0.a1 Prupe.1G143400_v2.0.a1 Prupe.1G143400_v2.0.a1 Prupe.1G143400_v2.0.a1
pyrus_communis	pycom13g15060 pycom13g15070 pycom16g14800 pycom16g14810
rosa_chinensis	RchiOBHm_Chr4g0420721
rosa_laevigata	RLG00000007740 RLG00000030269 RLG00000030290
rosa_multiflora	Rmu_co8498373.1_g000001
rosa_roxburghii	Rroxscaffold_5G00363230
rosa_rugosa	Rorug04G0165700
rosa_samantha	Rh4AG227200 Rh4BG230000 Rh4CG242100 Rh4DG225500
rosa_wichuraiana	Rw4G019660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1053
AarI	CACCTGC	1 cut(s)	1450
Acc36I	ACCTGC	2 cut(s)	1450, 2603
Acc65I	GGTACC	1 cut(s)	1428
AccB1I	GGYRCC	1 cut(s)	1428
AccB7I	CCANNNNNTGG	1 cut(s)	564
AccIII	TCCGGA	4 cut(s)	1783, 1943, 2131, 2194
AciI	CCGC	9 cut(s)	230, 431, 645, 856, 2040, 2043, 2415, 2796, 2840
AclI	AACGTT	1 cut(s)	1044
AclWI	GGATC	8 cut(s)	549, 1775, 2029, 2030, 2176, 2325, 2374, 2716
AcoI	YGGCCR	1 cut(s)	2302
AcsI	RAATTY	3 cut(s)	996, 1339, 1814
AcuI	CTGAAG	7 cut(s)	197, 783, 831, 959, 963, 1149, 2836
AfaI	GTAC	7 cut(s)	257, 348, 417, 596, 1430, 2104, 2110
AfiI	CCNNNNNNNGG	8 cut(s)	435, 436, 564, 865, 1730, 2531, 2733, 2782
AflII	CTTAAG	1 cut(s)	715
AflIII	ACRYGT	2 cut(s)	756, 892
AgeI	ACCGGT	1 cut(s)	2478
AjnI	CCWGG	5 cut(s)	457, 535, 679, 1553, 2445
AjuI	GAANNNNNNNTTGG	2 cut(s)	1269, 1301
AleI	CACNNNNGTG	2 cut(s)	674, 2532
AloI	GAACNNNNNNTCC	4 cut(s)	1995, 2027, 2189, 2221
Alw21I	GWGCWC	1 cut(s)	691
Alw26I	GTCTC	3 cut(s)	925, 1627, 1730
AlwI	GGATC	8 cut(s)	549, 1775, 2029, 2030, 2176, 2325, 2374, 2716
AlwNI	CAGNNNCTG	1 cut(s)	314
Ama87I	CYCGRG	1 cut(s)	2694
Aor13HI	TCCGGA	4 cut(s)	1783, 1943, 2131, 2194
AoxI	GGCC	5 cut(s)	21, 30, 1596, 1743, 2302
ApeKI	GCWGC	9 cut(s)	314, 523, 642, 930, 1009, 1193, 2412, 2799, 2831
ApoI	RAATTY	3 cut(s)	996, 1339, 1814
AsiGI	ACCGGT	1 cut(s)	2478
Asp700I	GAANNNNTTC	4 cut(s)	615, 812, 1310, 2067
Asp718I	GGTACC	1 cut(s)	1428
AspLEI	GCGC	1 cut(s)	103
AspS9I	GGNCC	6 cut(s)	30, 355, 1567, 1596, 1743, 2438
AsuC2I	CCSGG	1 cut(s)	2828
AsuII	TTCGAA	2 cut(s)	1319, 1903
AvaI	CYCGRG	1 cut(s)	2694
AvaII	GGWCC	3 cut(s)	355, 1567, 2438
BaeI	ACNNNNGTAYC	2 cut(s)	399, 432
BalI	TGGCCA	1 cut(s)	2304
BanI	GGYRCC	1 cut(s)	1428
BanII	GRGCYC	1 cut(s)	82
BauI	CACGAG	1 cut(s)	2575
BbsI	GAAGAC	3 cut(s)	1017, 1083, 1149
Bbv12I	GWGCWC	1 cut(s)	691
BbvI	GCAGC	9 cut(s)	301, 535, 629, 942, 1021, 1180, 2399, 2811, 2818
BccI	CCATC	9 cut(s)	187, 1064, 1910, 1948, 1984, 2144, 2276, 2379, 2663
BceAI	ACGGC	1 cut(s)	1046
BcgI	CGANNNNNNTGC	2 cut(s)	52, 86
BciT130I	CCWGG	5 cut(s)	459, 537, 681, 1555, 2447
BciVI	GTATCC	2 cut(s)	2128, 2584
BclI	TGATCA	1 cut(s)	220
BcnI	CCSGG	1 cut(s)	2828
BcoDI	GTCTC	3 cut(s)	925, 1627, 1730
BfaI	CTAG	4 cut(s)	1509, 1559, 2079, 2100
BfmI	CTRYAG	1 cut(s)	1203
BfrI	CTTAAG	1 cut(s)	715
BfuAI	ACCTGC	2 cut(s)	1450, 2603
BfuI	GTATCC	2 cut(s)	2128, 2584
BglI	GCCNNNNNGGC	1 cut(s)	29
BglII	AGATCT	3 cut(s)	623, 1660, 2256
BmcAI	AGTACT	2 cut(s)	348, 2104
Bme1390I	CCNGG	6 cut(s)	459, 537, 681, 1555, 2447, 2828
Bme18I	GGWCC	3 cut(s)	355, 1567, 2438
BmeT110I	CYCGRG	1 cut(s)	2694
BmgT120I	GGNCC	6 cut(s)	30, 355, 1567, 1596, 1743, 2438
BmiI	GGNNCC	3 cut(s)	1430, 1569, 2439
BmrFI	CCNGG	6 cut(s)	459, 537, 681, 1555, 2447, 2828
BmrI	ACTGGG	1 cut(s)	1768
BmuI	ACTGGG	1 cut(s)	1768
BpiI	GAAGAC	3 cut(s)	1017, 1083, 1149
BplI	GAGNNNNNCTC	2 cut(s)	2740, 2772
BpmI	CTGGAG	5 cut(s)	143, 460, 480, 488, 2429
Bpu14I	TTCGAA	2 cut(s)	1319, 1903
BpuEI	CTTGAG	1 cut(s)	1355
BpuMI	CCSGG	1 cut(s)	2828
Bsa29I	ATCGAT	1 cut(s)	2187
BsaJI	CCNNGG	3 cut(s)	536, 1016, 2731
BsaWI	WCCGGW	7 cut(s)	302, 1431, 1783, 1943, 2131, 2194, 2478
BsaXI	ACNNNNNCTCC	2 cut(s)	1995, 2025
Bsc4I	CCNNNNNNNGG	8 cut(s)	435, 436, 564, 865, 1730, 2531, 2733, 2782
Bse118I	RCCGGY	1 cut(s)	2478
Bse1I	ACTGG	6 cut(s)	203, 598, 906, 1763, 1996, 2564
Bse3DI	GCAATG	2 cut(s)	160, 657
BseAI	TCCGGA	4 cut(s)	1783, 1943, 2131, 2194
BseBI	CCWGG	5 cut(s)	459, 537, 681, 1555, 2447
BseCI	ATCGAT	1 cut(s)	2187
BseDI	CCNNGG	3 cut(s)	536, 1016, 2731
BseLI	CCNNNNNNNGG	8 cut(s)	435, 436, 564, 865, 1730, 2531, 2733, 2782
BseMI	GCAATG	2 cut(s)	160, 657
BseMII	CTCAG	9 cut(s)	36, 149, 162, 180, 783, 1524, 2193, 2738, 2818
BseNI	ACTGG	6 cut(s)	203, 598, 906, 1763, 1996, 2564
BseRI	GAGGAG	4 cut(s)	32, 1120, 1172, 2411
BseXI	GCAGC	9 cut(s)	301, 535, 629, 942, 1021, 1180, 2399, 2811, 2818
BseYI	CCCAGC	2 cut(s)	1454, 2814
BsgI	GTGCAG	2 cut(s)	327, 2639
BshFI	GGCC	5 cut(s)	23, 32, 1598, 1745, 2304
BshNI	GGYRCC	1 cut(s)	1428
BshTI	ACCGGT	1 cut(s)	2478
BshVI	ATCGAT	1 cut(s)	2187
BsiHKAI	GWGCWC	1 cut(s)	691
BsiHKCI	CYCGRG	1 cut(s)	2694
BsiSI	CCGG	9 cut(s)	303, 1432, 1565, 1784, 1944, 2132, 2195, 2479, 2828
BslFI	GGGAC	1 cut(s)	199
BslI	CCNNNNNNNGG	8 cut(s)	435, 436, 564, 865, 1730, 2531, 2733, 2782
BsmAI	GTCTC	3 cut(s)	925, 1627, 1730
BsmFI	GGGAC	1 cut(s)	199
BsmI	GAATGC	1 cut(s)	728
BsnI	GGCC	5 cut(s)	23, 32, 1598, 1745, 2304
BsoBI	CYCGRG	1 cut(s)	2694
Bsp119I	TTCGAA	2 cut(s)	1319, 1903
Bsp1286I	GDGCHC	2 cut(s)	82, 691
Bsp13I	TCCGGA	4 cut(s)	1783, 1943, 2131, 2194
BspACI	CCGC	9 cut(s)	230, 431, 645, 856, 2040, 2043, 2415, 2796, 2840
BspANI	GGCC	5 cut(s)	23, 32, 1598, 1745, 2304
BspCNI	CTCAG	9 cut(s)	37, 148, 163, 181, 784, 1525, 2194, 2739, 2817
BspDI	ATCGAT	1 cut(s)	2187
BspEI	TCCGGA	4 cut(s)	1783, 1943, 2131, 2194
BspLI	GGNNCC	3 cut(s)	1430, 1569, 2439
BspMI	ACCTGC	2 cut(s)	1450, 2603
BspPI	GGATC	8 cut(s)	549, 1775, 2029, 2030, 2176, 2325, 2374, 2716
BspQI	GCTCTTC	1 cut(s)	850
BspT104I	TTCGAA	2 cut(s)	1319, 1903
BspT107I	GGYRCC	1 cut(s)	1428
BspTI	CTTAAG	1 cut(s)	715
BsrDI	GCAATG	2 cut(s)	160, 657
BsrFI	RCCGGY	1 cut(s)	2478
BsrI	ACTGG	6 cut(s)	203, 598, 906, 1763, 1996, 2564
BssAI	RCCGGY	1 cut(s)	2478
BssECI	CCNNGG	3 cut(s)	536, 1016, 2731
BssSI	CACGAG	1 cut(s)	2575
Bst2BI	CACGAG	1 cut(s)	2575
Bst2UI	CCWGG	5 cut(s)	459, 537, 681, 1555, 2447
Bst4CI	ACNGT	2 cut(s)	359, 2728
Bst6I	CTCTTC	5 cut(s)	633, 850, 1097, 1125, 1245
BstAFI	CTTAAG	1 cut(s)	715
BstAPI	GCANNNNNTGC	2 cut(s)	314, 2354
BstBI	TTCGAA	2 cut(s)	1319, 1903
BstC8I	GCNNGC	5 cut(s)	374, 574, 726, 2288, 2355
BstDSI	CCRYGG	2 cut(s)	1016, 2731
BstHHI	GCGC	1 cut(s)	103
BstMAI	GTCTC	3 cut(s)	925, 1627, 1730
BstMWI	GCNNNNNNNGC	6 cut(s)	29, 98, 314, 523, 1199, 2354
BstNI	CCWGG	5 cut(s)	459, 537, 681, 1555, 2447
BstNSI	RCATGY	2 cut(s)	376, 760
BstSCI	CCNGG	6 cut(s)	457, 535, 679, 1553, 2445, 2826
BstSFI	CTRYAG	1 cut(s)	1203
BstV1I	GCAGC	9 cut(s)	301, 535, 629, 942, 1021, 1180, 2399, 2811, 2818
BstV2I	GAAGAC	3 cut(s)	1017, 1083, 1149
BstX2I	RGATCY	4 cut(s)	623, 1660, 2021, 2256
BstYI	RGATCY	4 cut(s)	623, 1660, 2021, 2256
Bsu15I	ATCGAT	1 cut(s)	2187
BsuI	GTATCC	2 cut(s)	2128, 2584
BsuRI	GGCC	5 cut(s)	23, 32, 1598, 1745, 2304
BsuTUI	ATCGAT	1 cut(s)	2187
BtgI	CCRYGG	2 cut(s)	1016, 2731
BtsI	GCAGTG	2 cut(s)	922, 940
BtsIMutI	CAGTG	4 cut(s)	913, 922, 940, 1125
BveI	ACCTGC	2 cut(s)	1450, 2603
Cac8I	GCNNGC	5 cut(s)	374, 574, 726, 2288, 2355
CaiI	CAGNNNCTG	1 cut(s)	314
CfoI	GCGC	1 cut(s)	103
Cfr10I	RCCGGY	1 cut(s)	2478
Cfr13I	GGNCC	6 cut(s)	30, 355, 1567, 1596, 1743, 2438
ClaI	ATCGAT	1 cut(s)	2187
CseI	GACGC	2 cut(s)	1154, 1208
Csp6I	GTAC	7 cut(s)	256, 347, 416, 595, 1429, 2103, 2109
CspAI	ACCGGT	1 cut(s)	2478
CviQI	GTAC	7 cut(s)	256, 347, 416, 595, 1429, 2103, 2109
EaeI	YGGCCR	1 cut(s)	2302
Eam1104I	CTCTTC	5 cut(s)	633, 850, 1097, 1125, 1245
EarI	CTCTTC	5 cut(s)	633, 850, 1097, 1125, 1245
EciI	GGCGGA	1 cut(s)	845
Eco24I	GRGCYC	1 cut(s)	82
Eco47I	GGWCC	3 cut(s)	355, 1567, 2438
Eco57I	CTGAAG	7 cut(s)	197, 783, 831, 959, 963, 1149, 2836
Eco88I	CYCGRG	1 cut(s)	2694
EcoRI	GAATTC	1 cut(s)	1339
EcoRII	CCWGG	5 cut(s)	457, 535, 679, 1553, 2445
EcoT22I	ATGCAT	1 cut(s)	378
EcoT38I	GRGCYC	1 cut(s)	82
FalI	AAGNNNNNCTT	4 cut(s)	603, 635, 829, 861
FaqI	GGGAC	1 cut(s)	199
FauI	CCCGC	3 cut(s)	438, 2047, 2408
FauNDI	CATATG	1 cut(s)	664
FbaI	TGATCA	1 cut(s)	220
FriOI	GRGCYC	1 cut(s)	82
FspBI	CTAG	4 cut(s)	1509, 1559, 2079, 2100
GlaI	GCGC	1 cut(s)	102
GsaI	CCCAGC	2 cut(s)	1458, 2818
GsuI	CTGGAG	5 cut(s)	143, 460, 480, 488, 2429
HaeIII	GGCC	5 cut(s)	23, 32, 1598, 1745, 2304
HapII	CCGG	9 cut(s)	303, 1432, 1565, 1784, 1944, 2132, 2195, 2479, 2828
HgaI	GACGC	2 cut(s)	1154, 1208
HhaI	GCGC	1 cut(s)	103
Hin6I	GCGC	1 cut(s)	101
HinP1I	GCGC	1 cut(s)	101
HincII	GTYRAC	1 cut(s)	754
HindII	GTYRAC	1 cut(s)	754
HindIII	AAGCTT	1 cut(s)	1885
HpaI	GTTAAC	1 cut(s)	754
HpaII	CCGG	9 cut(s)	303, 1432, 1565, 1784, 1944, 2132, 2195, 2479, 2828
Hpy166II	GTNNAC	4 cut(s)	416, 754, 1525, 2441
Hpy8I	GTNNAC	4 cut(s)	416, 754, 1525, 2441
HpyCH4III	ACNGT	2 cut(s)	359, 2728
HpyCH4IV	ACGT	2 cut(s)	892, 1044
HpyF10VI	GCNNNNNNNGC	6 cut(s)	29, 98, 314, 523, 1199, 2354
HpySE526I	ACGT	2 cut(s)	892, 1044
HspAI	GCGC	1 cut(s)	101
Kpn2I	TCCGGA	4 cut(s)	1783, 1943, 2131, 2194
KpnI	GGTACC	1 cut(s)	1432
Ksp22I	TGATCA	1 cut(s)	220
KspAI	GTTAAC	1 cut(s)	754
LguI	GCTCTTC	1 cut(s)	850
LmnI	GCTCC	4 cut(s)	369, 686, 788, 1364
Lsp1109I	GCAGC	9 cut(s)	301, 535, 629, 942, 1021, 1180, 2399, 2811, 2818
MaeI	CTAG	4 cut(s)	1509, 1559, 2079, 2100
MaeII	ACGT	2 cut(s)	892, 1044
MaeIII	GTNAC	5 cut(s)	359, 766, 1640, 1994, 2324
MflI	RGATCY	4 cut(s)	623, 1660, 2021, 2256
MhlI	GDGCHC	2 cut(s)	82, 691
MlsI	TGGCCA	1 cut(s)	2304
MluCI	AATT	8 cut(s)	176, 243, 996, 1339, 1628, 1814, 1949, 2336
MluNI	TGGCCA	1 cut(s)	2304
MlyI	GAGTC	2 cut(s)	1781, 2686
MmeI	TCCRAC	5 cut(s)	298, 425, 766, 1831, 2607
Mox20I	TGGCCA	1 cut(s)	2304
Mph1103I	ATGCAT	1 cut(s)	378
MroI	TCCGGA	4 cut(s)	1783, 1943, 2131, 2194
MroXI	GAANNNNTTC	4 cut(s)	615, 812, 1310, 2067
MscI	TGGCCA	1 cut(s)	2304
MslI	CAYNNNNRTG	4 cut(s)	674, 1065, 2532, 2612
Msp20I	TGGCCA	1 cut(s)	2304
MspA1I	CMGCKG	4 cut(s)	230, 2412, 2714, 2779
MspCI	CTTAAG	1 cut(s)	715
MspI	CCGG	9 cut(s)	303, 1432, 1565, 1784, 1944, 2132, 2195, 2479, 2828
MspR9I	CCNGG	6 cut(s)	459, 537, 681, 1555, 2447, 2828
Mva1269I	GAATGC	1 cut(s)	728
MvaI	CCWGG	5 cut(s)	459, 537, 681, 1555, 2447
MwoI	GCNNNNNNNGC	6 cut(s)	29, 98, 314, 523, 1199, 2354
NciI	CCSGG	1 cut(s)	2828
NdeI	CATATG	1 cut(s)	664
NlaIV	GGNNCC	3 cut(s)	1430, 1569, 2439
NmuCI	GTSAC	5 cut(s)	359, 766, 1640, 1994, 2324
NsiI	ATGCAT	1 cut(s)	378
NspI	RCATGY	2 cut(s)	376, 760
NspV	TTCGAA	2 cut(s)	1319, 1903
OliI	CACNNNNGTG	2 cut(s)	674, 2532
PaeI	GCATGC	1 cut(s)	376
PaqCI	CACCTGC	1 cut(s)	1450
PciI	ACATGT	1 cut(s)	756
PciSI	GCTCTTC	1 cut(s)	850
PctI	GAATGC	1 cut(s)	728
PdmI	GAANNNNTTC	4 cut(s)	615, 812, 1310, 2067
PfeI	GAWTC	8 cut(s)	902, 938, 1163, 1316, 1500, 1512, 2456, 2764
PflFI	GACNNNGTC	1 cut(s)	1638
PflMI	CCANNNNNTGG	1 cut(s)	564
PfoI	TCCNGGA	2 cut(s)	457, 2445
PinAI	ACCGGT	1 cut(s)	2478
PleI	GAGTC	2 cut(s)	1781, 2686
PpsI	GAGTC	2 cut(s)	1781, 2686
PscI	ACATGT	1 cut(s)	756
PsiI	TTATAA	1 cut(s)	1053
Psp1406I	AACGTT	1 cut(s)	1044
Psp6I	CCWGG	5 cut(s)	457, 535, 679, 1553, 2445
PspFI	CCCAGC	2 cut(s)	1454, 2814
PspGI	CCWGG	5 cut(s)	457, 535, 679, 1553, 2445
PspN4I	GGNNCC	3 cut(s)	1430, 1569, 2439
PspPI	GGNCC	6 cut(s)	30, 355, 1567, 1596, 1743, 2438
PstNI	CAGNNNCTG	1 cut(s)	314
PsuI	RGATCY	4 cut(s)	623, 1660, 2021, 2256
PsyI	GACNNNGTC	1 cut(s)	1638
PvuII	CAGCTG	3 cut(s)	2412, 2714, 2779
RsaI	GTAC	7 cut(s)	257, 348, 417, 596, 1430, 2104, 2110
RsaNI	GTAC	7 cut(s)	256, 347, 416, 595, 1429, 2103, 2109
RseI	CAYNNNNRTG	4 cut(s)	674, 1065, 2532, 2612
SapI	GCTCTTC	1 cut(s)	850
Sau96I	GGNCC	6 cut(s)	30, 355, 1567, 1596, 1743, 2438
ScaI	AGTACT	2 cut(s)	348, 2104
SchI	GAGTC	2 cut(s)	1781, 2686
ScrFI	CCNGG	6 cut(s)	459, 537, 681, 1555, 2447, 2828
SduI	GDGCHC	2 cut(s)	82, 691
SfcI	CTRYAG	1 cut(s)	1203
SfiI	GGCCNNNNNGGCC	1 cut(s)	29
SfuI	TTCGAA	2 cut(s)	1319, 1903
SinI	GGWCC	3 cut(s)	355, 1567, 2438
SmiMI	CAYNNNNRTG	4 cut(s)	674, 1065, 2532, 2612
SmlI	CTYRAG	2 cut(s)	715, 1334
SmoI	CTYRAG	2 cut(s)	715, 1334
SphI	GCATGC	1 cut(s)	376
Sse9I	AATT	8 cut(s)	176, 243, 996, 1339, 1628, 1814, 1949, 2336
SsiI	CCGC	9 cut(s)	230, 431, 645, 856, 2040, 2043, 2415, 2796, 2840
SspI	AATATT	1 cut(s)	2059
SspMI	CTAG	4 cut(s)	1509, 1559, 2079, 2100
StyD4I	CCNGG	6 cut(s)	457, 535, 679, 1553, 2445, 2826
TaaI	ACNGT	2 cut(s)	359, 2728
TaiI	ACGT	2 cut(s)	895, 1047
TaqI	TCGA	6 cut(s)	38, 1314, 1319, 1483, 1903, 2187
TasI	AATT	8 cut(s)	176, 243, 996, 1339, 1628, 1814, 1949, 2336
TatI	WGTACW	3 cut(s)	255, 346, 2102
TauI	GCSGC	3 cut(s)	2045, 2799, 2842
TfiI	GAWTC	8 cut(s)	902, 938, 1163, 1316, 1500, 1512, 2456, 2764
TscAI	CASTG	4 cut(s)	913, 922, 940, 1132
TseFI	GTSAC	5 cut(s)	359, 766, 1640, 1994, 2324
TseI	GCWGC	9 cut(s)	314, 523, 642, 930, 1009, 1193, 2412, 2799, 2831
Tsp45I	GTSAC	5 cut(s)	359, 766, 1640, 1994, 2324
TspDTI	ATGAA	7 cut(s)	269, 646, 1104, 1187, 1599, 1827, 2160
TspGWI	ACGGA	6 cut(s)	382, 1033, 2101, 2108, 2720, 2798
TspRI	CASTG	4 cut(s)	913, 922, 940, 1132
Tth111I	GACNNNGTC	1 cut(s)	1638
Van91I	CCANNNNNTGG	1 cut(s)	564
Vha464I	CTTAAG	1 cut(s)	715
VpaK11BI	GGWCC	3 cut(s)	355, 1567, 2438
XapI	RAATTY	3 cut(s)	996, 1339, 1814
XbaI	TCTAGA	1 cut(s)	2078
XceI	RCATGY	2 cut(s)	376, 760
XmnI	GAANNNNTTC	4 cut(s)	615, 812, 1310, 2067
XspI	CTAG	4 cut(s)	1509, 1559, 2079, 2100
ZrmI	AGTACT	2 cut(s)	348, 2104
Zsp2I	ATGCAT	1 cut(s)	378

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.