MD13G1186600.v1.1

Protein FANTASTIC FOUR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014046
Physical Location & Seq
Reverse (-)
15890683 .. 15891744
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1186600.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGCGACAATCGTGTGCCAAGGTCTGCAGTCATGCCTGGATTCTCCTCACCTTGTGGAGCCAAGAACCCTAAGGCTAAAGCTTTCTGCACCGGTAGCTCATCACTTCTCTCCAATTACCCCCCAAGAATTAGGAATCAAAGCTTGTTTCTCTGAAGAGAAATGCAATAATTATGAGGAAATCAACAGCAATAAGGGCACCTCCAAAAAATCTGACATGGGTGGCTGGAGTTTCCTCCAAGCTATCTCCAAACCCTCACTAGAAAACCCTACTGCTGAGGTGGACAAAGAAAATACTTACACCCCTCCTAGGGTTTATAGGAACTCATCAGCTACTAAGCTAAATGAGAAGAGCTTGGAGTTGTGCACTGAAGACTTGGGCTGCGAGACCGGCGCCATTATCACAGAAAGCTTCAGCATTTTCTCACCAATGTATGAATCAGAATCGAATGCAGCGGCAAGGGAGCATCAAAATAAGCATAAATTTTCGGGTGCAAAGAAGGCGAATAATGTTCACAATTTCCCACCTCCATTGACAACAATAAGCGGCGAGGATTCTCTACACTTTAGGCCTCACAGGGAAGATGGGAGGCTGATCATCAAAGCTGTCAAGGCCCCTTTAAGTCATTCATGCTTCCAAGCTGAAAGAAGCAATGGCCGCCTTCGATTGTGCCTTTCAAAAACCTTTACTCCCAGTTTTGACTCAGAGGAAGAAGCAGCAGCCATTCAAGAATACAATGAAGGGTGTGAAAATGACATAATTGAGGAGGAAACAGAAGAGCAAGATCAAGAAGTGGTGGGAGAAAATACAGAAGAAGAATTGGTAGAACAATTAGAAGGCGATAAGGAGGAGGAAGAGGAAGAAGAAGAAGAAGAAGAAGAAGGGAATAGTTCAAATGTGTGTTTGATAGAGGGTGAGGAGGTGGATGGGAATAGTTTGAATGTTAGGGGTAAAGATGGAATAGAAAAGTTTGAAAGGCCAAGCAGGTGCAAAGAAGGTGGCGGTGAGTGTGAGAAGAAAGGATTGTTAAAGTATTGGGAGCCCTTTTGGGTGGCTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAF

Protein Analysis

354

Amino Acids

39.5

Weight (kDa)

4.67

Isoelectric Point (pI)

65.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAF PF11250 173 - 225 1.8e-22 Fantastic Four meristem regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014046)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06020 AT5G19260
fragaria_vesca FvH4_4g14940
malus_domestica MD13G1186600.v1.1 MD16G1187300.v1.1
prunus_persica Prupe.1G154300_v2.0.a1
pyrus_communis pycom13g16150
rosa_chinensis RchiOBHm_Chr4g0417161
rosa_laevigata RLG00000007925
rosa_multiflora Rmu_sc0000646.1_g000019
rosa_roxburghii Rroxscaffold_5G00360980
rosa_rugosa Rorug04G0146200
rosa_samantha Rh4AG209100 Rh4BG206000 Rh4CG220100 Rh4DG206800
rosa_wichuraiana Rw4G017710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 975
Acc36I ACCTGC 1 cut(s) 975
AccB1I GGYRCC 2 cut(s) 197, 392
AciI CCGC 4 cut(s) 455, 546, 658, 1002
AcoI YGGCCR 1 cut(s) 655
AcsI RAATTY 1 cut(s) 482
AcuI CTGAAG 3 cut(s) 174, 390, 397
AcyI GRCGYC 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 309, 310
AgeI ACCGGT 1 cut(s) 91
AgsI TTSAA 5 cut(s) 678, 728, 894, 940, 974
AjnI CCWGG 1 cut(s) 36
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 1 cut(s) 380
Alw44I GTGCAC 1 cut(s) 364
AoxI GGCC 4 cut(s) 569, 612, 655, 977
ApaLI GTGCAC 1 cut(s) 364
ApeKI GCWGC 4 cut(s) 381, 452, 716, 719
ApoI RAATTY 1 cut(s) 482
AsiGI ACCGGT 1 cut(s) 91
AspA2I CCTAGG 1 cut(s) 308
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 613
AsuHPI GGTGA 4 cut(s) 41, 417, 926, 1016
AvrII CCTAGG 1 cut(s) 308
AxyI CCTNAGG 1 cut(s) 71
BaeGI GKGCMC 2 cut(s) 200, 368
BanI GGYRCC 2 cut(s) 197, 392
BanII GRGCYC 1 cut(s) 1044
BbsI GAAGAC 1 cut(s) 378
Bbv12I GWGCWC 1 cut(s) 368
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 4 cut(s) 368, 464, 728, 731
BccI CCATC 3 cut(s) 578, 920, 950
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 2 cut(s) 260, 309
BfmI CTRYAG 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 975
BisI GCNGC 7 cut(s) 382, 453, 456, 547, 658, 717, 720
BlnI CCTAGG 1 cut(s) 308
BlsI GCNGC 7 cut(s) 383, 454, 457, 548, 659, 718, 721
Bme1390I CCNGG 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 613
BmiI GGNNCC 5 cut(s) 60, 199, 394, 615, 1041
BmrFI CCNGG 1 cut(s) 38
BmrI ACTGGG 1 cut(s) 687
BmsI GCATC 1 cut(s) 475
BmuI ACTGGG 1 cut(s) 687
BpiI GAAGAC 1 cut(s) 378
BpmI CTGGAG 1 cut(s) 247
Bpu10I CCTNAGC 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 2 cut(s) 19, 308
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 673, 703
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 310
Bse118I RCCGGY 2 cut(s) 91, 389
Bse1I ACTGG 1 cut(s) 693
Bse21I CCTNAGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 658
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 2 cut(s) 19, 308
BseGI GGATG 1 cut(s) 931
BseLI CCNNNNNNNGG 2 cut(s) 309, 310
BseMI GCAATG 1 cut(s) 658
BseMII CTCAG 2 cut(s) 267, 717
BseNI ACTGG 1 cut(s) 693
BseRI GAGGAG 4 cut(s) 36, 779, 863, 932
BseSI GKGCMC 2 cut(s) 200, 368
BseXI GCAGC 4 cut(s) 368, 464, 728, 731
BsgI GTGCAG 1 cut(s) 72
BshFI GGCC 4 cut(s) 571, 614, 657, 979
BshNI GGYRCC 2 cut(s) 197, 392
BshTI ACCGGT 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 2 cut(s) 92, 390
BslI CCNNNNNNNGG 2 cut(s) 309, 310
BsmAI GTCTC 1 cut(s) 380
BsmI GAATGC 1 cut(s) 454
BsnI GGCC 4 cut(s) 571, 614, 657, 979
Bso31I GGTCTC 1 cut(s) 380
Bsp1286I GDGCHC 3 cut(s) 200, 368, 1044
Bsp143I GATC 2 cut(s) 594, 784
BspACI CCGC 4 cut(s) 455, 546, 658, 1002
BspANI GGCC 4 cut(s) 571, 614, 657, 979
BspCNI CTCAG 2 cut(s) 268, 716
BspLI GGNNCC 5 cut(s) 60, 199, 394, 615, 1041
BspMAI CTGCAG 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 975
BspQI GCTCTTC 2 cut(s) 344, 771
BspT107I GGYRCC 2 cut(s) 197, 392
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 1 cut(s) 658
BsrFI RCCGGY 2 cut(s) 91, 389
BsrI ACTGG 1 cut(s) 693
BssAI RCCGGY 2 cut(s) 91, 389
BssECI CCNNGG 2 cut(s) 19, 308
BssMI GATC 2 cut(s) 594, 784
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 2 cut(s) 19, 308
Bst2UI CCWGG 1 cut(s) 38
Bst6I CTCTTC 4 cut(s) 150, 344, 771, 849
BstACI GRCGYC 1 cut(s) 393
BstDEI CTNAG 4 cut(s) 71, 276, 336, 703
BstF5I GGATG 1 cut(s) 931
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 2 cut(s) 597, 787
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 2 cut(s) 594, 784
BstMWI GCNNNNNNNGC 6 cut(s) 95, 195, 390, 500, 611, 657
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 26
BstSLI GKGCMC 2 cut(s) 200, 368
BstV1I GCAGC 4 cut(s) 368, 464, 728, 731
BstV2I GAAGAC 1 cut(s) 378
Bsu36I CCTNAGG 1 cut(s) 71
BsuRI GGCC 4 cut(s) 571, 614, 657, 979
BtsCI GGATG 1 cut(s) 931
BtsIMutI CAGTG 1 cut(s) 366
BveI ACCTGC 1 cut(s) 975
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 2 cut(s) 91, 389
Cfr13I GGNCC 1 cut(s) 613
CspAI ACCGGT 1 cut(s) 91
CspCI CAANNNNNGTGG 4 cut(s) 513, 548, 979, 1014
CviAII CATG 3 cut(s) 33, 217, 630
DdeI CTNAG 4 cut(s) 71, 276, 336, 703
DinI GGCGCC 1 cut(s) 394
DpnI GATC 2 cut(s) 596, 786
DpnII GATC 2 cut(s) 594, 784
DraIII CACNNNGTG 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 655
Eam1104I CTCTTC 4 cut(s) 150, 344, 771, 849
EarI CTCTTC 4 cut(s) 150, 344, 771, 849
Eco130I CCWWGG 2 cut(s) 19, 308
Eco147I AGGCCT 1 cut(s) 571
Eco24I GRGCYC 1 cut(s) 1044
Eco31I GGTCTC 1 cut(s) 380
Eco57I CTGAAG 3 cut(s) 174, 390, 397
Eco81I CCTNAGG 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 2 cut(s) 19, 308
EcoT38I GRGCYC 1 cut(s) 1044
EgeI GGCGCC 1 cut(s) 394
EheI GGCGCC 1 cut(s) 394
ErhI CCWWGG 2 cut(s) 19, 308
FaeI CATG 3 cut(s) 36, 220, 633
FaiI YATR 8 cut(s) 34, 174, 218, 318, 435, 480, 631, 758
FatI CATG 3 cut(s) 32, 216, 629
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 7 cut(s) 382, 453, 456, 547, 658, 717, 720
FokI GGATG 1 cut(s) 938
FriOI GRGCYC 1 cut(s) 1044
Fsp4HI GCNGC 7 cut(s) 382, 453, 456, 547, 658, 717, 720
FspBI CTAG 2 cut(s) 260, 309
GlaI GCGC 1 cut(s) 394
GluI GCNGC 7 cut(s) 382, 453, 456, 547, 658, 717, 720
GsuI CTGGAG 1 cut(s) 247
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 4 cut(s) 571, 614, 657, 979
HapII CCGG 2 cut(s) 92, 390
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 3 cut(s) 36, 220, 633
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HindIII AAGCTT 3 cut(s) 80, 141, 409
HinfI GANTC 6 cut(s) 41, 135, 437, 443, 554, 701
HpaII CCGG 2 cut(s) 92, 390
HphI GGTGA 4 cut(s) 41, 417, 926, 1016
Hpy166II GTNNAC 3 cut(s) 283, 366, 514
Hpy188I TCNGA 4 cut(s) 154, 214, 442, 706
Hpy188III TCNNGA 2 cut(s) 728, 788
Hpy8I GTNNAC 3 cut(s) 283, 366, 514
HpyAV CCTTC 6 cut(s) 493, 671, 734, 830, 875, 989
HpyCH4V TGCA 7 cut(s) 28, 89, 165, 366, 452, 494, 990
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 195, 390, 500, 611, 657
HpyF3I CTNAG 4 cut(s) 71, 276, 336, 703
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 3 cut(s) 36, 220, 633
HspAI GCGC 1 cut(s) 393
KasI GGCGCC 1 cut(s) 392
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 2 cut(s) 594, 784
LguI GCTCTTC 2 cut(s) 344, 771
LmnI GCTCC 3 cut(s) 58, 463, 1039
LpnPI CCDG 8 cut(s) 23, 50, 105, 211, 403, 562, 706, 970
Lsp1109I GCAGC 4 cut(s) 368, 464, 728, 731
LweI GCATC 1 cut(s) 475
MaeI CTAG 2 cut(s) 260, 309
MalI GATC 2 cut(s) 596, 786
MboI GATC 2 cut(s) 594, 784
MhlI GDGCHC 3 cut(s) 200, 368, 1044
MluCI AATT 8 cut(s) 114, 128, 169, 482, 517, 759, 818, 830
Mly113I GGCGCC 1 cut(s) 393
MlyI GAGTC 1 cut(s) 695
MseI TTAA 3 cut(s) 620, 1028, 1060
MspA1I CMGCKG 1 cut(s) 455
MspI CCGG 2 cut(s) 92, 390
MspR9I CCNGG 1 cut(s) 38
Mva1269I GAATGC 1 cut(s) 454
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 6 cut(s) 95, 195, 390, 500, 611, 657
NarI GGCGCC 1 cut(s) 393
NdeII GATC 2 cut(s) 594, 784
NlaIII CATG 3 cut(s) 36, 220, 633
NlaIV GGNNCC 5 cut(s) 60, 199, 394, 615, 1041
PaqCI CACCTGC 1 cut(s) 975
PceI AGGCCT 1 cut(s) 571
PciSI GCTCTTC 2 cut(s) 344, 771
PctI GAATGC 1 cut(s) 454
PfeI GAWTC 5 cut(s) 41, 135, 437, 443, 554
PinAI ACCGGT 1 cut(s) 91
PkrI GCNGC 7 cut(s) 383, 454, 457, 548, 659, 718, 721
PleI GAGTC 1 cut(s) 695
PluTI GGCGCC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 695
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 5 cut(s) 60, 199, 394, 615, 1041
PspPI GGNCC 1 cut(s) 613
PstI CTGCAG 1 cut(s) 30
SapI GCTCTTC 2 cut(s) 344, 771
SaqAI TTAA 3 cut(s) 620, 1028, 1060
SatI GCNGC 7 cut(s) 382, 453, 456, 547, 658, 717, 720
Sau3AI GATC 2 cut(s) 594, 784
Sau96I GGNCC 1 cut(s) 613
SchI GAGTC 1 cut(s) 695
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 3 cut(s) 200, 368, 1044
SfaNI GCATC 1 cut(s) 475
SfcI CTRYAG 1 cut(s) 26
SfoI GGCGCC 1 cut(s) 394
Sse9I AATT 8 cut(s) 114, 128, 169, 482, 517, 759, 818, 830
SseBI AGGCCT 1 cut(s) 571
SsiI CCGC 4 cut(s) 455, 546, 658, 1002
SspDI GGCGCC 1 cut(s) 392
SspMI CTAG 2 cut(s) 260, 309
StuI AGGCCT 1 cut(s) 571
StyD4I CCNGG 1 cut(s) 36
StyI CCWWGG 2 cut(s) 19, 308
TaqI TCGA 2 cut(s) 446, 664
TasI AATT 8 cut(s) 114, 128, 169, 482, 517, 759, 818, 830
TauI GCSGC 3 cut(s) 458, 549, 660
TfiI GAWTC 5 cut(s) 41, 135, 437, 443, 554
Tru1I TTAA 3 cut(s) 620, 1028, 1060
Tru9I TTAA 3 cut(s) 620, 1028, 1060
TscAI CASTG 1 cut(s) 373
TseI GCWGC 4 cut(s) 381, 452, 716, 719
TspDTI ATGAA 3 cut(s) 450, 618, 753
TspRI CASTG 1 cut(s) 373
VneI GTGCAC 1 cut(s) 364
XapI RAATTY 1 cut(s) 482
XmaJI CCTAGG 1 cut(s) 308
XspI CTAG 2 cut(s) 260, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.