Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013912

Physical Location & Seq

Genomic Coordinates Reverse (-)

20175379 .. 20178781

Gene Structure 3403 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1214000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGGCGAAAGAACCAGCTCGCGTCCTTGTCACTGGAGCCGCAGGACAAATTGGATATGCGCTTGTGCCAATGATCGCAAGGGGAATCATGCTCGGAGCGGATCAGCCAGTGATTCTGCACTTGCTCGATATTCCACCAGCTGCTGAGGCTTTGAATGGTGTCAAGATGGAGTTGGTGGATGCCGCTTTCCCTCTGCTTAAAGGTGTTGTTGCTACAACTGATGTTGTAGAGGCATGCACTGGAGTCAACATCGCCGTGATGGTGGGTGGCTTCCCAAGGAAAGAAGGCATGGAAAGGAAAGACGTCATGACAAAGAACGTTTCCATCTACAAGTCCCAAGCTTCTGCACTGGAAAAGCATGCCGCTCCCAACTGCAAGGTTTTGGTTGTTGCTAATCCCGCAAACACCAATGCATTGATCTTGAAGGAATTTGCACCATCAATTCCTGAGAAAAACATCACCTGTCTGACTAGACTAGATCATAACAGGGCGTTGGGCCAGGTCTCAGAGAGATTGAATGTTCAAGTTTCCGATGTGAAGAATGTTATTATCTGGGGAAACCATTCATCATCTCAGTATCCTGATGTCAACCATGCAACTGTCAAGACACCATCTGGAGAGAAATGTGTCCGTGAGCTTGTTGCTGATGATGCATGGTTGAATGGAGAATTCATTTCCACTGTTCAACAACGTGGTGCTGCAATCATCAAGGCCCGTAAGCTGTCAAGTGCATTATCTGCTGCAAGTTCTGCTTGTGATCACATTCGTGATTGGGTACTTGGAACCCCAGAGGGCACTTGGGTTTCCATGGGTGTCTACTCAGACGGCTCATACAATGTACCTTCGGGTCTCATTTTTTCATTCCCAGTCACTTGCCAAAATGGAGAGTGGAAGATTGTTCAAGGACTCTCTATTGACGAGTTTTCGAGGAAGAAGTTGGATGCAACTGCAGATGAGCTTAGTGAGGAGAAAGCTTTGGCATACTCTTGCCTCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006107 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0016999 GO:0017144 GO:0019362 GO:0019637 GO:0019674 GO:0019752 GO:0030060 GO:0034641 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044464 GO:0045333 GO:0046483 GO:0046496 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072524 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko04964 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200 map04964

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

333

Amino Acids

35.54

Weight (kDa)

6.1

Isoelectric Point (pI)

29.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	7 - 153	3.9e-32	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	157 - 325	1.5e-45	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013912)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04410 AT5G43330
fragaria_vesca	FvH4_4g03630
malus_domestica	MD13G1214000.v1.1 MD16G1219000.v1.1
prunus_persica	Prupe.1G039300_v2.0.a1 Prupe.1G039300_v2.0.a1
pyrus_communis	pycom13g18720 pycom16g18250
rosa_chinensis	RchiOBHm_Chr4g0393011
rosa_laevigata	RLG00000009775
rosa_multiflora	Rmu_sc0008339.1_g000014
rosa_roxburghii	Rroxscaffold_5G00338110
rosa_rugosa	Rorug04G0003000
rosa_samantha	Rh4BG041300 Rh4CG048900
rosa_wichuraiana	Rw4G003570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	306
AccBSI	CCGCTC	2 cut(s)	98, 365
AccI	GTMKAC	1 cut(s)	816
AccII	CGCG	1 cut(s)	21
AciI	CCGC	5 cut(s)	39, 98, 183, 363, 399
AclI	AACGTT	1 cut(s)	318
AclWI	GGATC	1 cut(s)	108
AcsI	RAATTY	2 cut(s)	428, 668
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	2 cut(s)	777, 840
AgsI	TTSAA	7 cut(s)	154, 424, 517, 524, 661, 686, 902
AjnI	CCWGG	1 cut(s)	498
AleI	CACNNNNGTG	1 cut(s)	765
AluBI	AGCT	7 cut(s)	17, 140, 341, 637, 721, 958, 974
AluI	AGCT	7 cut(s)	17, 140, 341, 637, 721, 958, 974
Alw26I	GTCTC	2 cut(s)	508, 854
AlwI	GGATC	1 cut(s)	108
AlwNI	CAGNNNCTG	1 cut(s)	143
AoxI	GGCC	2 cut(s)	496, 711
ApeKI	GCWGC	3 cut(s)	140, 698, 740
ApoI	RAATTY	2 cut(s)	428, 668
Asp700I	GAANNNNTTC	1 cut(s)	562
AspLEI	GCGC	1 cut(s)	61
AspS9I	GGNCC	2 cut(s)	496, 712
AsuHPI	GGTGA	1 cut(s)	451
BaeGI	GKGCMC	1 cut(s)	797
BbvCI	CCTCAGC	1 cut(s)	144
BbvI	GCAGC	3 cut(s)	127, 685, 727
BccI	CCATC	5 cut(s)	160, 253, 332, 445, 619
BceAI	ACGGC	2 cut(s)	239, 841
BciT130I	CCWGG	1 cut(s)	500
BciVI	GTATCC	1 cut(s)	588
BclI	TGATCA	1 cut(s)	757
BcoDI	GTCTC	2 cut(s)	508, 854
BfaI	CTAG	2 cut(s)	471, 476
BfmI	CTRYAG	1 cut(s)	948
BfuI	GTATCC	1 cut(s)	588
BisI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
BlsI	GCNGC	6 cut(s)	40, 142, 184, 364, 700, 742
Bme1390I	CCNGG	1 cut(s)	500
BmgT120I	GGNCC	2 cut(s)	496, 712
BmiI	GGNNCC	2 cut(s)	37, 784
BmrFI	CCNGG	1 cut(s)	500
BmrI	ACTGGG	1 cut(s)	860
BmsI	GCATC	3 cut(s)	169, 640, 931
BmuI	ACTGGG	1 cut(s)	860
BpmI	CTGGAG	3 cut(s)	54, 261, 636
Bpu10I	CCTNAGC	1 cut(s)	144
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	2 cut(s)	508, 854
BsaJI	CCNNGG	2 cut(s)	275, 807
Bse1I	ACTGG	5 cut(s)	37, 107, 244, 354, 866
BseBI	CCWGG	1 cut(s)	500
BseDI	CCNNGG	2 cut(s)	275, 807
BseGI	GGATG	2 cut(s)	184, 946
BseMII	CTCAG	5 cut(s)	135, 438, 519, 587, 834
BseNI	ACTGG	5 cut(s)	37, 107, 244, 354, 866
BseRI	GAGGAG	1 cut(s)	980
BseSI	GKGCMC	1 cut(s)	797
BseXI	GCAGC	3 cut(s)	127, 685, 727
BsgI	GTGCAG	2 cut(s)	101, 330
Bsh1236I	CGCG	1 cut(s)	21
BshFI	GGCC	2 cut(s)	498, 713
BslFI	GGGAC	1 cut(s)	319
BsmAI	GTCTC	2 cut(s)	508, 854
BsmFI	GGGAC	1 cut(s)	319
BsnI	GGCC	2 cut(s)	498, 713
Bso31I	GGTCTC	2 cut(s)	508, 854
Bsp1286I	GDGCHC	1 cut(s)	797
Bsp143I	GATC	5 cut(s)	72, 100, 417, 478, 757
Bsp19I	CCATGG	1 cut(s)	807
BspACI	CCGC	5 cut(s)	39, 98, 183, 363, 399
BspANI	GGCC	2 cut(s)	498, 713
BspCNI	CTCAG	5 cut(s)	136, 439, 518, 586, 833
BspFNI	CGCG	1 cut(s)	21
BspHI	TCATGA	1 cut(s)	306
BspLI	GGNNCC	2 cut(s)	37, 784
BspMAI	CTGCAG	1 cut(s)	952
BspPI	GGATC	1 cut(s)	108
BspTNI	GGTCTC	2 cut(s)	508, 854
BsrBI	CCGCTC	2 cut(s)	98, 365
BsrI	ACTGG	5 cut(s)	37, 107, 244, 354, 866
BssECI	CCNNGG	2 cut(s)	275, 807
BssMI	GATC	5 cut(s)	72, 100, 417, 478, 757
BssNI	GRCGYC	1 cut(s)	303
BssT1I	CCWWGG	2 cut(s)	275, 807
Bst2UI	CCWGG	1 cut(s)	500
Bst4CI	ACNGT	2 cut(s)	601, 682
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	2 cut(s)	737, 749
BstC8I	GCNNGC	3 cut(s)	19, 235, 360
BstDEI	CTNAG	7 cut(s)	144, 447, 505, 573, 820, 959, 996
BstDSI	CCRYGG	1 cut(s)	807
BstF5I	GGATG	2 cut(s)	184, 946
BstFNI	CGCG	1 cut(s)	21
BstHHI	GCGC	1 cut(s)	61
BstKTI	GATC	5 cut(s)	75, 103, 420, 481, 760
BstMAI	GTCTC	2 cut(s)	508, 854
BstMBI	GATC	5 cut(s)	72, 100, 417, 478, 757
BstMWI	GCNNNNNNNGC	5 cut(s)	146, 398, 650, 737, 749
BstNI	CCWGG	1 cut(s)	500
BstNSI	RCATGY	2 cut(s)	237, 362
BstSCI	CCNGG	1 cut(s)	498
BstSFI	CTRYAG	1 cut(s)	948
BstSLI	GKGCMC	1 cut(s)	797
BstUI	CGCG	1 cut(s)	21
BstV1I	GCAGC	3 cut(s)	127, 685, 727
BsuI	GTATCC	1 cut(s)	588
BsuRI	GGCC	2 cut(s)	498, 713
BtgI	CCRYGG	1 cut(s)	807
BtgZI	GCGATG	1 cut(s)	235
BtsCI	GGATG	2 cut(s)	184, 946
BtsIMutI	CAGTG	5 cut(s)	30, 114, 237, 347, 678
Cac8I	GCNNGC	3 cut(s)	19, 235, 360
CaiI	CAGNNNCTG	1 cut(s)	143
CciI	TCATGA	1 cut(s)	306
CfoI	GCGC	1 cut(s)	61
Cfr13I	GGNCC	2 cut(s)	496, 712
CseI	GACGC	1 cut(s)	10
Csp6I	GTAC	2 cut(s)	776, 839
CviAII	CATG	8 cut(s)	88, 234, 289, 307, 359, 593, 654, 808
CviQI	GTAC	2 cut(s)	776, 839
DdeI	CTNAG	7 cut(s)	144, 447, 505, 573, 820, 959, 996
DpnI	GATC	5 cut(s)	74, 102, 419, 480, 759
DpnII	GATC	5 cut(s)	72, 100, 417, 478, 757
Eco130I	CCWWGG	2 cut(s)	275, 807
Eco31I	GGTCTC	2 cut(s)	508, 854
EcoRI	GAATTC	1 cut(s)	668
EcoRII	CCWGG	1 cut(s)	498
EcoT14I	CCWWGG	2 cut(s)	275, 807
EcoT22I	ATGCAT	2 cut(s)	415, 655
ErhI	CCWWGG	2 cut(s)	275, 807
FaeI	CATG	8 cut(s)	91, 237, 292, 310, 362, 596, 657, 811
FalI	AAGNNNNNCTT	2 cut(s)	736, 768
FaqI	GGGAC	1 cut(s)	319
FatI	CATG	8 cut(s)	87, 233, 288, 306, 358, 592, 653, 807
FauI	CCCGC	1 cut(s)	406
FbaI	TGATCA	1 cut(s)	757
FblI	GTMKAC	1 cut(s)	816
Fnu4HI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
FokI	GGATG	2 cut(s)	191, 953
Fsp4HI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
FspBI	CTAG	2 cut(s)	471, 476
GlaI	GCGC	1 cut(s)	60
GluI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
GsuI	CTGGAG	3 cut(s)	54, 261, 636
HaeIII	GGCC	2 cut(s)	498, 713
HgaI	GACGC	1 cut(s)	10
HhaI	GCGC	1 cut(s)	61
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	8 cut(s)	91, 237, 292, 310, 362, 596, 657, 811
Hin6I	GCGC	1 cut(s)	59
HinP1I	GCGC	1 cut(s)	59
HincII	GTYRAC	2 cut(s)	247, 589
HindII	GTYRAC	2 cut(s)	247, 589
HindIII	AAGCTT	2 cut(s)	339, 972
HinfI	GANTC	4 cut(s)	84, 112, 243, 906
HphI	GGTGA	1 cut(s)	451
Hpy166II	GTNNAC	3 cut(s)	247, 589, 817
Hpy188I	TCNGA	5 cut(s)	95, 468, 508, 532, 823
Hpy188III	TCNNGA	8 cut(s)	163, 307, 421, 446, 581, 604, 615, 767
Hpy8I	GTNNAC	3 cut(s)	247, 589, 817
HpyAV	CCTTC	3 cut(s)	278, 418, 852
HpyCH4III	ACNGT	2 cut(s)	601, 682
HpyCH4IV	ACGT	3 cut(s)	303, 318, 691
HpyF10VI	GCNNNNNNNGC	5 cut(s)	146, 398, 650, 737, 749
HpyF3I	CTNAG	7 cut(s)	144, 447, 505, 573, 820, 959, 996
HpySE526I	ACGT	3 cut(s)	303, 318, 691
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	8 cut(s)	91, 237, 292, 310, 362, 596, 657, 811
HspAI	GCGC	1 cut(s)	59
Ksp22I	TGATCA	1 cut(s)	757
Kzo9I	GATC	5 cut(s)	72, 100, 417, 478, 757
LmnI	GCTCC	3 cut(s)	35, 95, 370
Lsp1109I	GCAGC	3 cut(s)	127, 685, 727
LweI	GCATC	3 cut(s)	169, 640, 931
MaeI	CTAG	2 cut(s)	471, 476
MaeII	ACGT	3 cut(s)	303, 318, 691
MaeIII	GTNAC	2 cut(s)	28, 868
MalI	GATC	5 cut(s)	74, 102, 419, 480, 759
MbiI	CCGCTC	2 cut(s)	98, 365
MboI	GATC	5 cut(s)	72, 100, 417, 478, 757
MboII	GAAGA	3 cut(s)	550, 904, 943
MhlI	GDGCHC	1 cut(s)	797
MluCI	AATT	4 cut(s)	48, 428, 441, 668
MlyI	GAGTC	2 cut(s)	252, 900
MmeI	TCCRAC	1 cut(s)	918
MnlI	CCTC	6 cut(s)	139, 201, 223, 784, 921, 958
Mph1103I	ATGCAT	2 cut(s)	415, 655
MroXI	GAANNNNTTC	1 cut(s)	562
MseI	TTAA	1 cut(s)	198
MslI	CAYNNNNRTG	2 cut(s)	254, 765
MspA1I	CMGCKG	1 cut(s)	140
MspR9I	CCNGG	1 cut(s)	500
MvaI	CCWGG	1 cut(s)	500
MvnI	CGCG	1 cut(s)	21
MwoI	GCNNNNNNNGC	5 cut(s)	146, 398, 650, 737, 749
NcoI	CCATGG	1 cut(s)	807
NdeII	GATC	5 cut(s)	72, 100, 417, 478, 757
NlaIII	CATG	8 cut(s)	91, 237, 292, 310, 362, 596, 657, 811
NlaIV	GGNNCC	2 cut(s)	37, 784
NmuCI	GTSAC	2 cut(s)	28, 868
NsiI	ATGCAT	2 cut(s)	415, 655
NspI	RCATGY	2 cut(s)	237, 362
OliI	CACNNNNGTG	1 cut(s)	765
PaeI	GCATGC	2 cut(s)	237, 362
PagI	TCATGA	1 cut(s)	306
PdmI	GAANNNNTTC	1 cut(s)	562
PfeI	GAWTC	2 cut(s)	84, 112
PkrI	GCNGC	6 cut(s)	40, 142, 184, 364, 700, 742
PleI	GAGTC	2 cut(s)	251, 900
PpsI	GAGTC	2 cut(s)	251, 900
Psp1406I	AACGTT	1 cut(s)	318
Psp6I	CCWGG	1 cut(s)	498
PspGI	CCWGG	1 cut(s)	498
PspN4I	GGNNCC	2 cut(s)	37, 784
PspPI	GGNCC	2 cut(s)	496, 712
PstI	CTGCAG	1 cut(s)	952
PstNI	CAGNNNCTG	1 cut(s)	143
PvuII	CAGCTG	1 cut(s)	140
RsaI	GTAC	2 cut(s)	777, 840
RsaNI	GTAC	2 cut(s)	776, 839
RseI	CAYNNNNRTG	2 cut(s)	254, 765
SaqAI	TTAA	1 cut(s)	198
SatI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
Sau3AI	GATC	5 cut(s)	72, 100, 417, 478, 757
Sau96I	GGNCC	2 cut(s)	496, 712
SchI	GAGTC	2 cut(s)	252, 900
ScrFI	CCNGG	1 cut(s)	500
SduI	GDGCHC	1 cut(s)	797
SfaNI	GCATC	3 cut(s)	169, 640, 931
SfcI	CTRYAG	1 cut(s)	948
SmiMI	CAYNNNNRTG	2 cut(s)	254, 765
SphI	GCATGC	2 cut(s)	237, 362
Sse9I	AATT	4 cut(s)	48, 428, 441, 668
SsiI	CCGC	5 cut(s)	39, 98, 183, 363, 399
SspMI	CTAG	2 cut(s)	471, 476
StyD4I	CCNGG	1 cut(s)	498
StyI	CCWWGG	2 cut(s)	275, 807
TaaI	ACNGT	2 cut(s)	601, 682
TaiI	ACGT	3 cut(s)	306, 321, 694
TaqI	TCGA	2 cut(s)	126, 926
TasI	AATT	4 cut(s)	48, 428, 441, 668
TauI	GCSGC	3 cut(s)	41, 185, 365
TfiI	GAWTC	2 cut(s)	84, 112
Tru1I	TTAA	1 cut(s)	198
Tru9I	TTAA	1 cut(s)	198
TscAI	CASTG	5 cut(s)	37, 114, 244, 354, 685
TseFI	GTSAC	2 cut(s)	28, 868
TseI	GCWGC	3 cut(s)	140, 698, 740
Tsp45I	GTSAC	2 cut(s)	28, 868
TspDTI	ATGAA	3 cut(s)	555, 661, 849
TspGWI	ACGGA	1 cut(s)	620
TspRI	CASTG	5 cut(s)	37, 114, 244, 354, 685
XapI	RAATTY	2 cut(s)	428, 668
XceI	RCATGY	2 cut(s)	237, 362
XcmI	CCANNNNNNNNNTGG	1 cut(s)	795
XmiI	GTMKAC	1 cut(s)	816
XmnI	GAANNNNTTC	1 cut(s)	562
XspI	CTAG	2 cut(s)	471, 476
ZraI	GACGTC	1 cut(s)	304
Zsp2I	ATGCAT	2 cut(s)	415, 655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.