Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0013912

Physical Location & Seq

Genomic Coordinates Reverse (-)

14851320 .. 14853819

Gene Structure 2500 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g18250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGGCGAAACAACCAGCTCGCATCCTCGTTACTGGAGCCGCAGGGCAAATTGGATATGCGCTTGTGCCGATGATTGCAAGGGGAATCATGCTCGGAGCGGATCAGCCGGTGATTCTGCACTTGCTTGATATTCCACCAGCTGCTGAGGCTTTGAATGGTGTCAAGATGGAGTTGGTGGACGCCGCTTTCCCTCTGCTTAAAGGTGTTGTTGCTACAACTGATGTTGTGGAGGCATGCACTGGAGTTAACATCGCCGTCATGGTGGGTGGCTTCCCAAGGAAAGAGGGCATGGAAAGGAAAGACGTCATGACAAAGAACGTTTCCATCTACAGGTCCCAAGCTTCTGCACTGGAAAAGCATGCAGCTCCCAACTGCAAGGTTTTGGTTGTTGCTAATCCCGCAAACACCAATGCATTGATCTTGAAGGAATTTGCACCATCAATTCCTGAGAAAAACATCACCTGTTTGACTAGACTAGATCATAACAGGGCCTTGGGCCAGGTTTCTGAGAGATTGAATGTTCAAGTTTCTGATGTTAAGAATGTTATTATCTGGGGTAACCACTCATCGTCGCAGTATCCTGATGTCAACCATGCAACTGTCAAGACACCATCTGGGGAGAAAGGTGTCCGTGAGCTTGTTGCTGATGATGCATGGTTGAATGGAGAATTCATTTCCACTGTTCAACAACGTGGTGCTGCAATCATCAAGGCCCGTAAGCTGTCAAGTGCATTATCTGCTGCAAGTTCTGCTTGTGATCACATTCGTGATTGGGTACTTGGAACCCCGGAGGGCACTTGGGTTTCCATGGGTGTTTACTCTGACGGCTCATACGATGTACCTCCTGGTCTCATTTTCTCATTCCCAGTCACTTGCCAACATGGGGAGTGGAAGATTGTTCAAGGACTCTCTATTGACGAGTTCTCGAGGAAGAAGTTGAATGCAACTGCAGAGGAGCTGAGTGAGGAGAAAGCTTTGGCATACTCTTGCCTCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0006107 GO:0006108 GO:0006139 GO:0006725 GO:0006732 GO:0006733 GO:0006734 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009060 GO:0009117 GO:0009987 GO:0015980 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0016999 GO:0017144 GO:0019362 GO:0019637 GO:0019674 GO:0019752 GO:0030060 GO:0034641 GO:0043436 GO:0043648 GO:0044237 GO:0044238 GO:0044281 GO:0044424 GO:0044464 GO:0045333 GO:0046483 GO:0046496 GO:0051186 GO:0055086 GO:0055114 GO:0071704 GO:0072350 GO:0072524 GO:1901360 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00020 ko00270 ko00620 ko00630 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko04964 map00020 map00270 map00620 map00630 map00710 map01100 map01110 map01120 map01130 map01200 map04964

Pfam Domains

Protein Families

Ldh_1_C Ldh_1_N

Protein Analysis

333

Amino Acids

35.58

Weight (kDa)

6.4

Isoelectric Point (pI)

33.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ldh_1_N	PF00056	7 - 153	8.3e-32	lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C	PF02866	157 - 325	2.7e-45	lactate/malate dehydrogenase, alpha/beta C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013912)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04410 AT5G43330
fragaria_vesca	FvH4_4g03630
malus_domestica	MD13G1214000.v1.1 MD16G1219000.v1.1
prunus_persica	Prupe.1G039300_v2.0.a1 Prupe.1G039300_v2.0.a1
pyrus_communis	pycom13g18720 pycom16g18250
rosa_chinensis	RchiOBHm_Chr4g0393011
rosa_laevigata	RLG00000009775
rosa_multiflora	Rmu_sc0008339.1_g000014
rosa_roxburghii	Rroxscaffold_5G00338110
rosa_rugosa	Rorug04G0003000
rosa_samantha	Rh4BG041300 Rh4CG048900
rosa_wichuraiana	Rw4G003570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	306
AccBSI	CCGCTC	1 cut(s)	98
AciI	CCGC	4 cut(s)	39, 98, 183, 399
AclI	AACGTT	1 cut(s)	318
AclWI	GGATC	1 cut(s)	108
AcsI	RAATTY	2 cut(s)	428, 668
AcyI	GRCGYC	2 cut(s)	180, 303
AfaI	GTAC	2 cut(s)	777, 840
AfiI	CCNNNNNNNGG	2 cut(s)	330, 883
AgsI	TTSAA	8 cut(s)	154, 424, 517, 524, 661, 686, 902, 940
AjnI	CCWGG	2 cut(s)	498, 844
AleI	CACNNNNGTG	1 cut(s)	765
AluBI	AGCT	8 cut(s)	17, 140, 341, 365, 637, 721, 958, 974
AluI	AGCT	8 cut(s)	17, 140, 341, 365, 637, 721, 958, 974
Alw26I	GTCTC	1 cut(s)	854
AlwI	GGATC	1 cut(s)	108
AlwNI	CAGNNNCTG	1 cut(s)	143
Ama87I	CYCGRG	1 cut(s)	925
AoxI	GGCC	3 cut(s)	489, 496, 711
ApeKI	GCWGC	4 cut(s)	140, 362, 698, 740
ApoI	RAATTY	2 cut(s)	428, 668
AspLEI	GCGC	1 cut(s)	61
AspS9I	GGNCC	4 cut(s)	333, 489, 496, 712
AsuC2I	CCSGG	1 cut(s)	788
AsuHPI	GGTGA	2 cut(s)	121, 451
AvaI	CYCGRG	1 cut(s)	925
AvaII	GGWCC	1 cut(s)	333
BaeGI	GKGCMC	1 cut(s)	797
BbvCI	CCTCAGC	1 cut(s)	144
BbvI	GCAGC	4 cut(s)	127, 374, 685, 727
BccI	CCATC	4 cut(s)	160, 332, 445, 619
BceAI	ACGGC	2 cut(s)	239, 841
BciT130I	CCWGG	2 cut(s)	500, 846
BciVI	GTATCC	1 cut(s)	588
BclI	TGATCA	1 cut(s)	757
BcnI	CCSGG	1 cut(s)	788
BcoDI	GTCTC	1 cut(s)	854
BfaI	CTAG	2 cut(s)	471, 476
BfmI	CTRYAG	2 cut(s)	328, 948
BfuI	GTATCC	1 cut(s)	588
BisI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
BlsI	GCNGC	6 cut(s)	40, 142, 184, 364, 700, 742
Bme1390I	CCNGG	3 cut(s)	500, 788, 846
Bme18I	GGWCC	1 cut(s)	333
BmeT110I	CYCGRG	1 cut(s)	925
BmgT120I	GGNCC	4 cut(s)	333, 489, 496, 712
BmiI	GGNNCC	3 cut(s)	37, 335, 784
BmrFI	CCNGG	3 cut(s)	500, 788, 846
BmrI	ACTGGG	1 cut(s)	860
BmsI	GCATC	2 cut(s)	30, 640
BmuI	ACTGGG	1 cut(s)	860
BpmI	CTGGAG	2 cut(s)	54, 261
Bpu10I	CCTNAGC	1 cut(s)	144
BpuMI	CCSGG	1 cut(s)	788
BsaHI	GRCGYC	2 cut(s)	180, 303
BsaI	GGTCTC	1 cut(s)	854
BsaJI	CCNNGG	4 cut(s)	275, 492, 786, 807
Bsc4I	CCNNNNNNNGG	2 cut(s)	330, 883
Bse118I	RCCGGY	1 cut(s)	106
Bse1I	ACTGG	4 cut(s)	37, 244, 354, 866
BseBI	CCWGG	2 cut(s)	500, 846
BseDI	CCNNGG	4 cut(s)	275, 492, 786, 807
BseGI	GGATG	1 cut(s)	21
BseLI	CCNNNNNNNGG	2 cut(s)	330, 883
BseMII	CTCAG	4 cut(s)	135, 438, 498, 950
BseNI	ACTGG	4 cut(s)	37, 244, 354, 866
BseRI	GAGGAG	2 cut(s)	968, 980
BseSI	GKGCMC	1 cut(s)	797
BseXI	GCAGC	4 cut(s)	127, 374, 685, 727
BsgI	GTGCAG	2 cut(s)	101, 330
BshFI	GGCC	3 cut(s)	491, 498, 713
BsiHKCI	CYCGRG	1 cut(s)	925
BsiSI	CCGG	2 cut(s)	107, 788
BslFI	GGGAC	1 cut(s)	319
BslI	CCNNNNNNNGG	2 cut(s)	330, 883
BsmAI	GTCTC	1 cut(s)	854
BsmFI	GGGAC	1 cut(s)	319
BsmI	GAATGC	1 cut(s)	946
BsnI	GGCC	3 cut(s)	491, 498, 713
Bso31I	GGTCTC	1 cut(s)	854
BsoBI	CYCGRG	1 cut(s)	925
Bsp1286I	GDGCHC	1 cut(s)	797
Bsp143I	GATC	4 cut(s)	100, 417, 478, 757
Bsp19I	CCATGG	1 cut(s)	807
BspACI	CCGC	4 cut(s)	39, 98, 183, 399
BspANI	GGCC	3 cut(s)	491, 498, 713
BspCNI	CTCAG	4 cut(s)	136, 439, 499, 951
BspHI	TCATGA	1 cut(s)	306
BspLI	GGNNCC	3 cut(s)	37, 335, 784
BspMAI	CTGCAG	1 cut(s)	952
BspPI	GGATC	1 cut(s)	108
BspTNI	GGTCTC	1 cut(s)	854
BsrBI	CCGCTC	1 cut(s)	98
BsrFI	RCCGGY	1 cut(s)	106
BsrI	ACTGG	4 cut(s)	37, 244, 354, 866
BssAI	RCCGGY	1 cut(s)	106
BssECI	CCNNGG	4 cut(s)	275, 492, 786, 807
BssMI	GATC	4 cut(s)	100, 417, 478, 757
BssNI	GRCGYC	2 cut(s)	180, 303
BssT1I	CCWWGG	3 cut(s)	275, 492, 807
Bst2UI	CCWGG	2 cut(s)	500, 846
Bst4CI	ACNGT	2 cut(s)	601, 682
BstACI	GRCGYC	2 cut(s)	180, 303
BstAPI	GCANNNNNTGC	2 cut(s)	737, 749
BstC8I	GCNNGC	3 cut(s)	19, 235, 360
BstDEI	CTNAG	5 cut(s)	144, 447, 507, 959, 996
BstDSI	CCRYGG	1 cut(s)	807
BstEII	GGTNACC	1 cut(s)	557
BstF5I	GGATG	1 cut(s)	21
BstHHI	GCGC	1 cut(s)	61
BstKTI	GATC	4 cut(s)	103, 420, 481, 760
BstMAI	GTCTC	1 cut(s)	854
BstMBI	GATC	4 cut(s)	100, 417, 478, 757
BstMWI	GCNNNNNNNGC	5 cut(s)	146, 398, 650, 737, 749
BstNI	CCWGG	2 cut(s)	500, 846
BstNSI	RCATGY	2 cut(s)	237, 362
BstPI	GGTNACC	1 cut(s)	557
BstSCI	CCNGG	3 cut(s)	498, 786, 844
BstSFI	CTRYAG	2 cut(s)	328, 948
BstSLI	GKGCMC	1 cut(s)	797
BstV1I	GCAGC	4 cut(s)	127, 374, 685, 727
BsuI	GTATCC	1 cut(s)	588
BsuRI	GGCC	3 cut(s)	491, 498, 713
BtgI	CCRYGG	1 cut(s)	807
BtgZI	GCGATG	1 cut(s)	235
BtsCI	GGATG	1 cut(s)	21
BtsIMutI	CAGTG	3 cut(s)	237, 347, 678
Cac8I	GCNNGC	3 cut(s)	19, 235, 360
CaiI	CAGNNNCTG	1 cut(s)	143
CciI	TCATGA	1 cut(s)	306
CfoI	GCGC	1 cut(s)	61
Cfr10I	RCCGGY	1 cut(s)	106
Cfr13I	GGNCC	4 cut(s)	333, 489, 496, 712
CseI	GACGC	1 cut(s)	188
Csp6I	GTAC	2 cut(s)	776, 839
CviQI	GTAC	2 cut(s)	776, 839
DdeI	CTNAG	5 cut(s)	144, 447, 507, 959, 996
DpnI	GATC	4 cut(s)	102, 419, 480, 759
DpnII	GATC	4 cut(s)	100, 417, 478, 757
Eco130I	CCWWGG	3 cut(s)	275, 492, 807
Eco31I	GGTCTC	1 cut(s)	854
Eco47I	GGWCC	1 cut(s)	333
Eco88I	CYCGRG	1 cut(s)	925
Eco91I	GGTNACC	1 cut(s)	557
EcoO109I	RGGNCCY	2 cut(s)	333, 489
EcoO65I	GGTNACC	1 cut(s)	557
EcoRI	GAATTC	1 cut(s)	668
EcoRII	CCWGG	2 cut(s)	498, 844
EcoT14I	CCWWGG	3 cut(s)	275, 492, 807
EcoT22I	ATGCAT	2 cut(s)	415, 655
ErhI	CCWWGG	3 cut(s)	275, 492, 807
FalI	AAGNNNNNCTT	2 cut(s)	736, 768
FaqI	GGGAC	1 cut(s)	319
FauI	CCCGC	1 cut(s)	406
FbaI	TGATCA	1 cut(s)	757
Fnu4HI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
FokI	GGATG	1 cut(s)	8
Fsp4HI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
FspBI	CTAG	2 cut(s)	471, 476
GlaI	GCGC	1 cut(s)	60
GluI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
GsuI	CTGGAG	2 cut(s)	54, 261
HaeIII	GGCC	3 cut(s)	491, 498, 713
HapII	CCGG	2 cut(s)	107, 788
HgaI	GACGC	1 cut(s)	188
HhaI	GCGC	1 cut(s)	61
Hin1I	GRCGYC	2 cut(s)	180, 303
Hin6I	GCGC	1 cut(s)	59
HinP1I	GCGC	1 cut(s)	59
HincII	GTYRAC	2 cut(s)	247, 589
HindII	GTYRAC	2 cut(s)	247, 589
HindIII	AAGCTT	2 cut(s)	339, 972
HinfI	GANTC	3 cut(s)	84, 112, 906
HpaI	GTTAAC	1 cut(s)	247
HpaII	CCGG	2 cut(s)	107, 788
HphI	GGTGA	2 cut(s)	121, 451
Hpy166II	GTNNAC	4 cut(s)	178, 247, 589, 817
Hpy188I	TCNGA	4 cut(s)	95, 508, 532, 823
Hpy188III	TCNNGA	8 cut(s)	163, 307, 421, 446, 581, 604, 767, 925
Hpy8I	GTNNAC	4 cut(s)	178, 247, 589, 817
Hpy99I	CGWCG	1 cut(s)	574
HpyAV	CCTTC	1 cut(s)	418
HpyCH4III	ACNGT	2 cut(s)	601, 682
HpyCH4IV	ACGT	3 cut(s)	303, 318, 691
HpyF10VI	GCNNNNNNNGC	5 cut(s)	146, 398, 650, 737, 749
HpyF3I	CTNAG	5 cut(s)	144, 447, 507, 959, 996
HpySE526I	ACGT	3 cut(s)	303, 318, 691
Hsp92I	GRCGYC	2 cut(s)	180, 303
HspAI	GCGC	1 cut(s)	59
Ksp22I	TGATCA	1 cut(s)	757
KspAI	GTTAAC	1 cut(s)	247
Kzo9I	GATC	4 cut(s)	100, 417, 478, 757
LmnI	GCTCC	4 cut(s)	35, 95, 370, 955
Lsp1109I	GCAGC	4 cut(s)	127, 374, 685, 727
LweI	GCATC	2 cut(s)	30, 640
MaeI	CTAG	2 cut(s)	471, 476
MaeII	ACGT	3 cut(s)	303, 318, 691
MaeIII	GTNAC	3 cut(s)	28, 557, 868
MalI	GATC	4 cut(s)	102, 419, 480, 759
MbiI	CCGCTC	1 cut(s)	98
MboI	GATC	4 cut(s)	100, 417, 478, 757
MboII	GAAGA	2 cut(s)	904, 943
MhlI	GDGCHC	1 cut(s)	797
MluCI	AATT	4 cut(s)	48, 428, 441, 668
MlyI	GAGTC	1 cut(s)	900
Mph1103I	ATGCAT	2 cut(s)	415, 655
MseI	TTAA	3 cut(s)	198, 246, 537
MslI	CAYNNNNRTG	1 cut(s)	765
MspA1I	CMGCKG	1 cut(s)	140
MspI	CCGG	2 cut(s)	107, 788
MspR9I	CCNGG	3 cut(s)	500, 788, 846
Mva1269I	GAATGC	1 cut(s)	946
MvaI	CCWGG	2 cut(s)	500, 846
MwoI	GCNNNNNNNGC	5 cut(s)	146, 398, 650, 737, 749
NciI	CCSGG	1 cut(s)	788
NcoI	CCATGG	1 cut(s)	807
NdeII	GATC	4 cut(s)	100, 417, 478, 757
NlaIV	GGNNCC	3 cut(s)	37, 335, 784
NmuCI	GTSAC	1 cut(s)	868
NsiI	ATGCAT	2 cut(s)	415, 655
NspI	RCATGY	2 cut(s)	237, 362
OliI	CACNNNNGTG	1 cut(s)	765
PaeI	GCATGC	2 cut(s)	237, 362
PaeR7I	CTCGAG	1 cut(s)	925
PagI	TCATGA	1 cut(s)	306
PcsI	WCGNNNNNNNCGW	1 cut(s)	831
PctI	GAATGC	1 cut(s)	946
PfeI	GAWTC	2 cut(s)	84, 112
PkrI	GCNGC	6 cut(s)	40, 142, 184, 364, 700, 742
PleI	GAGTC	1 cut(s)	900
PpsI	GAGTC	1 cut(s)	900
PpuMI	RGGWCCY	1 cut(s)	333
Psp1406I	AACGTT	1 cut(s)	318
Psp5II	RGGWCCY	1 cut(s)	333
Psp6I	CCWGG	2 cut(s)	498, 844
PspEI	GGTNACC	1 cut(s)	557
PspGI	CCWGG	2 cut(s)	498, 844
PspN4I	GGNNCC	3 cut(s)	37, 335, 784
PspPI	GGNCC	4 cut(s)	333, 489, 496, 712
PspPPI	RGGWCCY	1 cut(s)	333
PstI	CTGCAG	1 cut(s)	952
PstNI	CAGNNNCTG	1 cut(s)	143
PvuII	CAGCTG	1 cut(s)	140
RsaI	GTAC	2 cut(s)	777, 840
RsaNI	GTAC	2 cut(s)	776, 839
RseI	CAYNNNNRTG	1 cut(s)	765
SaqAI	TTAA	3 cut(s)	198, 246, 537
SatI	GCNGC	6 cut(s)	39, 141, 183, 363, 699, 741
Sau3AI	GATC	4 cut(s)	100, 417, 478, 757
Sau96I	GGNCC	4 cut(s)	333, 489, 496, 712
SchI	GAGTC	1 cut(s)	900
ScrFI	CCNGG	3 cut(s)	500, 788, 846
SduI	GDGCHC	1 cut(s)	797
SfaNI	GCATC	2 cut(s)	30, 640
SfcI	CTRYAG	2 cut(s)	328, 948
Sfr274I	CTCGAG	1 cut(s)	925
SinI	GGWCC	1 cut(s)	333
SlaI	CTCGAG	1 cut(s)	925
SmiMI	CAYNNNNRTG	1 cut(s)	765
SmlI	CTYRAG	1 cut(s)	925
SmoI	CTYRAG	1 cut(s)	925
SphI	GCATGC	2 cut(s)	237, 362
Sse9I	AATT	4 cut(s)	48, 428, 441, 668
SsiI	CCGC	4 cut(s)	39, 98, 183, 399
SspMI	CTAG	2 cut(s)	471, 476
StyD4I	CCNGG	3 cut(s)	498, 786, 844
StyI	CCWWGG	3 cut(s)	275, 492, 807
TaaI	ACNGT	2 cut(s)	601, 682
TaiI	ACGT	3 cut(s)	306, 321, 694
TaqI	TCGA	1 cut(s)	926
TasI	AATT	4 cut(s)	48, 428, 441, 668
TauI	GCSGC	2 cut(s)	41, 185
TfiI	GAWTC	2 cut(s)	84, 112
Tru1I	TTAA	3 cut(s)	198, 246, 537
Tru9I	TTAA	3 cut(s)	198, 246, 537
TscAI	CASTG	3 cut(s)	244, 354, 685
TseFI	GTSAC	1 cut(s)	868
TseI	GCWGC	4 cut(s)	140, 362, 698, 740
Tsp45I	GTSAC	1 cut(s)	868
TspDTI	ATGAA	1 cut(s)	661
TspGWI	ACGGA	1 cut(s)	620
TspRI	CASTG	3 cut(s)	244, 354, 685
VpaK11BI	GGWCC	1 cut(s)	333
XapI	RAATTY	2 cut(s)	428, 668
XceI	RCATGY	2 cut(s)	237, 362
XhoI	CTCGAG	1 cut(s)	925
XspI	CTAG	2 cut(s)	471, 476
ZraI	GACGTC	1 cut(s)	304
Zsp2I	ATGCAT	2 cut(s)	415, 655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.