MD13G1219000.v1.1

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014182
Physical Location & Seq
Reverse (-)
21157814 .. 21162973
5160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1219000.v1.1.491

Sequence Viewer

Length: 3540 bp
ATGGGACCCCATCACCACTCTCGCCGCGAGTCCCTTTGCAGCTCCCTTCTCGCCCCCCGTCCGGACTACCACAACTCCGACCCTGACCTCGTCTGGCCCTTCGGCAAGCTCGAGGGCATCGACCGTGATGAGGTGCGCGAGACCACGTACGAGATCTTCTTCACGGCGTGTCGGTCATCCCCTGGCTTCGGGGGACGGAACGCCCTCGTGTTCTACTCCAACCACCAAGATAACAACAACAACAACAGCAATAGTAATGGTGGGGAGGGGAGCGGGTCGGGATCGGGGTCAAAGGTAAACGGGGTGGTGACTACACCGACGAGCCGGGTGAAGCGGGCGCTCGGGCTGAAGATGCTGAAGCGGTCTCCGTCGAGGAGGATGACTTCAGGTGCCGGAAATGGTGGATGGTCAAGTCCCTCCTCGCCAAACGGATCGAATAGTAGTGGCTCCCCCGGGATGTCGTACACCCTGCCGCCCTCGAGGCCGAGGCGGCCAATGACGTCGGCCGAGATCATGAGGCAGCAGATGAGGGTCACGGAAGGCAGCGATAATCGGCTGCGGAAGACGCTCATGAGGACCCTCGTAGGCCAAAAAAGTTTAAGCAATAACCCACCTCCACCGACGGTTGGCGAATTCATCCTCGACATCCCGTCTCTTCTCACTTATTTCCTTGTTTTCTCTCTCATCTTCCCTCCGTTTTCTCCTTCTCTTCGGCATGGGACCCAAGATGGTCGACGACAACTGCAATCCTTAAATCTGCTATCTCCGATTTCCGATATAAGAATCAATGAGGTCGCTGACCTCCCATATGTCCTTATCCAGCGAAAGAAAAAAAGGAAGAGAAAAGGAGAAAGGAAGGGAAAGGGAGAGAGAGAAATAGGTGAGGCATGGAGTTGCAAAAGATGGATGGGTGGGGTGAGGGTGTTGGGCGCAGAGGATCGGAATCTGGAAGGCCAGGTGTTTGATGCTCATTGGTCTGGAACACCAACTGTCACTAGTGAATTGGTATTCGTATCTAGAAAAATTTCTCCCAAAGTAAAAATGGGTAGGCGAGCAGAGACCATAATCCTCCCACTTGAACTCCTTCGACATCTAAAGCCATCCGAATTCAACGACTCCCACGAGTACCATTACTGGCAAAAGCGGCAGCTCAAAATCCTCGAAGCTGGTCTTCTTCTGCACCCTTCAATCCCACTAGACAAGTCCAACACATTCGCTTTGCGTCTCAGAGAGATCATCAGAGCGGGTGACACCAAAGCCATAGACACAGGAAAGAACTCAGACACCATGAGAACCCTTTGCAACTCTGTAGTCTCGTTATCGTGGCGTAGCTCCAACGGAACTCCAACAGATGTGTGTCACTGGGCTGATGGCTACCCCCTCAACATCCACCTCTATGTTGCTCTCCTACAATCAGTCTTCGACATCAGAGATGAGACATTAGTATTAGATGAAGTCGATGAACTGCTGGAGCTGATGAAGAAGACATGGTCGACCTTGGGGATCACTAGGCCAATTCACAATGTGTGCTTTACATGGGTGTTGTTTCAGCAATACGTGCAAACCGCACAGATTGAACCCGACCTTTTGTGTGCTGCTCATGCTATGCTTGCAGAAGTGGCGAACAATGCCAAGAGGCCTGATAGGGAGGCTTTGTATGTCAAGATTTTGTCTTCGGTGTTGAGCTCAATGCAGGGGTGGGCGGAGAAGAAATTGCTTCGGTACCATGATTATTTCCAAAGAGGTACAGTTGGCCAAATTGAGAACCTTCTCCCTTTGGCCTTGTCATCCTCCAAGATCTTGGGTGAAGATGTTACAATCACAGAAAGGGGAGGAGGAGTAAAAGGTGATATAAAGGTGGTGGACAACTCCGGTGACCGCGTGGACTACTACATTCGATCTTCTATGAAACAGGCTTTTGCAAAGATTATGGAAGCTGGAAATGTCACTGAAGTGGCAGAAGACGCCGTGACTGATACGTTGTTTCAGTTAGCTCAAGAGACAGAGGAGTTGGCCTTGAAAGAGAGGGAAAGCTTCAGTCCTATACTCAAGAGGTGGCACACAACTGCAGCTGGTGTCGCGGCTGTGACACTGCACAACTGCTATGGAGCTGTGTTGAAGCAATACCTAAACGGAGTCTCCACGCTTTCAGGTGAGACGGTTGAGATATTGCAAAGGGCAGGAAAGCTAGAAAAGGTTCTGCTCCAAATGGTGGTTGAGGACTCTGCTGAGTGCGAAGATGGTGGCAAAGCAATCATTAGAGAGATGGTTCCATATGAAGTTGATACCATCATAATGAACCTCTTAAAAAGATGGATTCATGAGAGATTAAAGAGAGGGAAAGAGTGTGTTCACTGTGCGAAAGAAAGCGAGACATGGAATCCAAAGTCCAAATCGGAACCTTATGCGCAATCAGCTGAGGAGCTAATGAAACTGGCCAAGGAAACCGTGGATGACTTCTTCGAAATTCCAATAGGAATTACTGAAGATATAGTTCAAGATCTTGCAGATGGGTTGGAGGATCTCTTCAAGGAGTATACTTCATTTGTCGCATCTTGTGGTTCGAAACAGAGTTATATCCCTACACTTCCTCCTCTAACAAGGTGCAATCGAGACTCGAAGTTCCTCAAGCTGTGGAAAAAAGCTAGCCCTTGTAGCATTGGAGCAGAAGGTTTTCACCCAAATGGAACAAATGATGGTCACCATCCTCGCCCATCAACGAGTAGAGGAACACAACGCCTCTACATCCGCCTCAACACTTTGCATTATCTCCTTCCCCACCTTCATTCGCTTGACAAAAACCTCTCCCTCTCACCTCGAATCATTCCTTCAACACCTCGCAGTCGTCCTGCCAAGAACCGCAAGAGTCACAGCAATGCCTCTTCCTACTTTGAATTTGCAGTGTCAGGCATCCAATCGGCCTGCCAACATGTCTCGGAAGTCGCTGCCTATCGCTTGATCTTCCTCGACTCAAACTCAGTCTTCTACGAGAGCCTCTATGTTGGTGATGTAGCCAATGCAAGAATAAGACCTGCGTTGCGAATTATCAAGCAGAATCTCACTCTCTTGGGAGCAATTCTCACCGACAAAGCTCAGGCCTTGGCGATCAAGGAAGTAATGAAGGCCTCTTTCGAAGCATTCCTCATGGTTTTGGTTGCTGGAGGAAGTTCCCGAGTGTTTTACCGAACTTACCATGAGATGATACAGGAAGATTTCGACAGCTTAAAGCGTGTTTTTTGCACTTGCGGAGAAGGGTTGATAGCCAAGGATGTGGTGGAACATGAGGCGGAGACAGCGGAAGGGGTGATAGAGCTGATGGGGCAATGTACTGAACAACTCGTGGAGGATTTCAGCATTGTGACATGCGAAAAAAGCGGGATTGGAGTTGTGGGATCAGGGCAAAGGCTGCCTATGCCTCCGACAACAGGACGGTGGAACAGATCAGATCCAAACACAATATTGAGGGTGTTATGCCACAGGAATGACAAAGCAGCAAACCAATTCTTGAAAAGGACATTCCAGCTAGCAAAGAGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

1180

Amino Acids

131.78

Weight (kDa)

8.9

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 765 - 1179 2.2e-181 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04470 AT2G33420
fragaria_vesca FvH4_4g04280 FvH4_4g04280
malus_domestica MD13G1219000.v1.1 MD16G1224300.v1.1
prunus_persica Prupe.1G045700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394731
rosa_laevigata RLG00000009667
rosa_multiflora Rmu_sc0005356.1_g000011
rosa_roxburghii Rroxscaffold_5G00339750
rosa_rugosa Rorug03G0362100.1
rosa_samantha Rh4AG054800 Rh4BG052500 Rh4CG059300 Rh4DG051100
rosa_wichuraiana Rw4G004370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 503
AbsI CCTCGAGG 1 cut(s) 478
Acc16I TGCGCA 1 cut(s) 2409
Acc36I ACCTGC 1 cut(s) 3040
Acc65I GGTACC 1 cut(s) 1722
AccB1I GGYRCC 2 cut(s) 389, 1722
AccB7I CCANNNNNTGG 1 cut(s) 2212
AccBSI CCGCTC 2 cut(s) 273, 1244
AccI GTMKAC 3 cut(s) 733, 1493, 2537
AccII CGCG 4 cut(s) 27, 138, 1881, 2081
AccIII TCCGGA 1 cut(s) 61
AclWI GGATC 7 cut(s) 289, 439, 945, 1511, 2529, 3400, 3440
AcoI YGGCCR 4 cut(s) 491, 504, 1753, 2436
AcsI RAATTY 5 cut(s) 632, 1023, 1106, 2466, 2894
AcuI CTGAAG 6 cut(s) 368, 369, 377, 1971, 2020, 2505
AcyI GRCGYC 2 cut(s) 500, 1965
AdeI CACNNNGTG 2 cut(s) 168, 1525
AfaI GTAC 6 cut(s) 149, 464, 1127, 1724, 1747, 3328
AfiI CCNNNNNNNGG 6 cut(s) 61, 130, 188, 626, 2212, 3425
AflIII ACRYGT 1 cut(s) 2929
AhdI GACNNNNNGTC 1 cut(s) 649
AhlI ACTAGT 1 cut(s) 995
AjnI CCWGG 2 cut(s) 181, 954
AleI CACNNNNGTG 1 cut(s) 1952
Alw21I GWGCWC 1 cut(s) 1688
AlwI GGATC 7 cut(s) 289, 439, 945, 1511, 2529, 3400, 3440
Ama87I CYCGRG 5 cut(s) 110, 341, 452, 478, 3171
Aor13HI TCCGGA 1 cut(s) 61
ApoI RAATTY 5 cut(s) 632, 1023, 1106, 2466, 2894
Asp700I GAANNNNTTC 4 cut(s) 1024, 1083, 2196, 2673
Asp718I GGTACC 1 cut(s) 1722
AspLEI GCGC 4 cut(s) 138, 340, 932, 2410
AspS9I GGNCC 4 cut(s) 5, 96, 576, 720
AsuC2I CCSGG 3 cut(s) 326, 453, 454
AsuII TTCGAA 3 cut(s) 2463, 2564, 3132
AsuNHI GCTAGC 2 cut(s) 2645, 3523
AvaI CYCGRG 5 cut(s) 110, 341, 452, 478, 3171
AvaII GGWCC 3 cut(s) 5, 576, 720
BalI TGGCCA 2 cut(s) 1755, 2438
BanI GGYRCC 2 cut(s) 389, 1722
BanII GRGCYC 1 cut(s) 1688
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BauI CACGAG 3 cut(s) 206, 1121, 3338
BbsI GAAGAC 7 cut(s) 569, 1163, 1411, 1490, 1665, 1968, 2974
Bbv12I GWGCWC 1 cut(s) 1688
BbvCI CCTCAGC 1 cut(s) 2418
BceAI ACGGC 2 cut(s) 180, 1952
BciT130I CCWGG 2 cut(s) 183, 956
BcnI CCSGG 3 cut(s) 326, 453, 454
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 7 cut(s) 996, 1017, 1196, 1509, 2189, 2646, 3524
BfmI CTRYAG 2 cut(s) 1308, 2067
BfoI RGCGCY 1 cut(s) 341
BfuAI ACCTGC 1 cut(s) 3040
BglI GCCNNNNNGGC 2 cut(s) 481, 490
BglII AGATCT 3 cut(s) 153, 1797, 2500
Bme1390I CCNGG 5 cut(s) 183, 326, 453, 454, 956
Bme18I GGWCC 3 cut(s) 5, 576, 720
BmeRI GACNNNNNGTC 1 cut(s) 649
BmeT110I CYCGRG 5 cut(s) 110, 341, 452, 478, 3171
BmgT120I GGNCC 4 cut(s) 5, 96, 576, 720
BmrFI CCNGG 5 cut(s) 183, 326, 453, 454, 956
BmrI ACTGGG 1 cut(s) 1372
BmsI GCATC 5 cut(s) 126, 342, 955, 2561, 2919
BmtI GCTAGC 2 cut(s) 2649, 3527
BmuI ACTGGG 1 cut(s) 1372
BpiI GAAGAC 7 cut(s) 569, 1163, 1411, 1490, 1665, 1968, 2974
BplI GAGNNNNNCTC 2 cut(s) 3063, 3095
BpmI CTGGAG 2 cut(s) 1490, 3180
Bpu10I CCTNAGC 2 cut(s) 2418, 3093
Bpu14I TTCGAA 3 cut(s) 2463, 2564, 3132
BpuEI CTTGAG 3 cut(s) 1980, 2033, 2612
BpuMI CCSGG 3 cut(s) 326, 453, 454
BsaAI YACGTR 2 cut(s) 147, 1558
BsaBI GATNNNNATC 1 cut(s) 942
BsaHI GRCGYC 2 cut(s) 500, 1965
BsaI GGTCTC 3 cut(s) 134, 369, 1052
BsaJI CCNNGG 9 cut(s) 181, 451, 452, 485, 1497, 2439, 2448, 3099, 3264
BsaWI WCCGGW 2 cut(s) 61, 1871
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 130, 188, 626, 2212, 3425
Bse1I ACTGG 3 cut(s) 1139, 1367, 2439
Bse3DI GCAATG 2 cut(s) 2881, 3329
Bse8I GATNNNNATC 1 cut(s) 942
BseAI TCCGGA 1 cut(s) 61
BseBI CCWGG 2 cut(s) 183, 956
BseDI CCNNGG 9 cut(s) 181, 451, 452, 485, 1497, 2439, 2448, 3099, 3264
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 6 cut(s) 61, 130, 188, 626, 2212, 3425
BseMI GCAATG 2 cut(s) 2881, 3329
BseMII CTCAG 6 cut(s) 1240, 1293, 2220, 2409, 2991, 3107
BseNI ACTGG 3 cut(s) 1139, 1367, 2439
BseRI GAGGAG 7 cut(s) 388, 409, 1848, 1851, 2021, 2435, 2583
BseX3I CGGCCG 1 cut(s) 504
BsgI GTGCAG 2 cut(s) 1163, 2078
Bsh1236I CGCG 4 cut(s) 27, 138, 1881, 2081
Bsh1285I CGRYCG 2 cut(s) 124, 507
BshNI GGYRCC 2 cut(s) 389, 1722
BsiEI CGRYCG 2 cut(s) 124, 507
BsiHKAI GWGCWC 1 cut(s) 1688
BsiHKCI CYCGRG 5 cut(s) 110, 341, 452, 478, 3171
BsiSI CCGG 5 cut(s) 62, 325, 393, 453, 1872
BsiWI CGTACG 1 cut(s) 147
BslFI GGGAC 5 cut(s) 16, 18, 207, 399, 733
BslI CCNNNNNNNGG 6 cut(s) 61, 130, 188, 626, 2212, 3425
BsmBI CGTCTC 3 cut(s) 657, 1229, 2150
BsmFI GGGAC 5 cut(s) 16, 18, 207, 399, 733
BsmI GAATGC 1 cut(s) 3137
Bso31I GGTCTC 3 cut(s) 134, 369, 1052
BsoBI CYCGRG 5 cut(s) 110, 341, 452, 478, 3171
Bsp119I TTCGAA 3 cut(s) 2463, 2564, 3132
Bsp1286I GDGCHC 1 cut(s) 1688
Bsp13I TCCGGA 1 cut(s) 61
BspCNI CTCAG 6 cut(s) 1239, 1292, 2221, 2410, 2990, 3106
BspEI TCCGGA 1 cut(s) 61
BspFNI CGCG 4 cut(s) 27, 138, 1881, 2081
BspHI TCATGA 3 cut(s) 513, 570, 2320
BspMAI CTGCAG 1 cut(s) 2071
BspMI ACCTGC 1 cut(s) 3040
BspOI GCTAGC 2 cut(s) 2649, 3527
BspPI GGATC 7 cut(s) 289, 439, 945, 1511, 2529, 3400, 3440
BspT104I TTCGAA 3 cut(s) 2463, 2564, 3132
BspT107I GGYRCC 2 cut(s) 389, 1722
BspTNI GGTCTC 3 cut(s) 134, 369, 1052
BsrBI CCGCTC 2 cut(s) 273, 1244
BsrDI GCAATG 2 cut(s) 2881, 3329
BsrI ACTGG 3 cut(s) 1139, 1367, 2439
BssECI CCNNGG 9 cut(s) 181, 451, 452, 485, 1497, 2439, 2448, 3099, 3264
BssNAI GTATAC 1 cut(s) 2538
BssNI GRCGYC 2 cut(s) 500, 1965
BssSI CACGAG 3 cut(s) 206, 1121, 3338
BssT1I CCWWGG 4 cut(s) 1497, 2439, 3099, 3264
Bst1107I GTATAC 1 cut(s) 2538
Bst2BI CACGAG 3 cut(s) 206, 1121, 3338
Bst2UI CCWGG 2 cut(s) 183, 956
Bst4CI ACNGT 8 cut(s) 125, 625, 991, 1750, 2161, 2357, 2449, 3432
Bst6I CTCTTC 5 cut(s) 660, 714, 833, 2531, 2887
BstACI GRCGYC 2 cut(s) 500, 1965
BstAPI GCANNNNNTGC 2 cut(s) 1558, 3406
BstBAI YACGTR 2 cut(s) 147, 1558
BstBI TTCGAA 3 cut(s) 2463, 2564, 3132
BstC8I GCNNGC 7 cut(s) 107, 336, 1053, 1611, 2647, 2923, 3525
BstDEI CTNAG 6 cut(s) 1226, 1279, 2229, 2418, 2977, 3093
BstDSI CCRYGG 1 cut(s) 2448
BstEII GGTNACC 2 cut(s) 1874, 2699
BstFNI CGCG 4 cut(s) 27, 138, 1881, 2081
BstH2I RGCGCY 1 cut(s) 341
BstHHI GCGC 4 cut(s) 138, 340, 932, 2410
BstMCI CGRYCG 2 cut(s) 124, 507
BstNI CCWGG 2 cut(s) 183, 956
BstNSI RCATGY 2 cut(s) 2933, 3366
BstPI GGTNACC 2 cut(s) 1874, 2699
BstSCI CCNGG 5 cut(s) 181, 324, 451, 452, 954
BstSFI CTRYAG 2 cut(s) 1308, 2067
BstUI CGCG 4 cut(s) 27, 138, 1881, 2081
BstV2I GAAGAC 7 cut(s) 569, 1163, 1411, 1490, 1665, 1968, 2974
BstX2I RGATCY 5 cut(s) 153, 1797, 2500, 2521, 3445
BstXI CCANNNNNNTGG 2 cut(s) 1801, 3271
BstYI RGATCY 5 cut(s) 153, 1797, 2500, 2521, 3445
BstZ17I GTATAC 1 cut(s) 2538
BstZI CGGCCG 1 cut(s) 504
BtgI CCRYGG 1 cut(s) 2448
BtsI GCAGTG 2 cut(s) 2090, 2907
BtsIMutI CAGTG 5 cut(s) 1360, 1947, 2090, 2353, 2907
BveI ACCTGC 1 cut(s) 3040
Cac8I GCNNGC 7 cut(s) 107, 336, 1053, 1611, 2647, 2923, 3525
CciI TCATGA 3 cut(s) 513, 570, 2320
CfoI GCGC 4 cut(s) 138, 340, 932, 2410
Cfr13I GGNCC 4 cut(s) 5, 96, 576, 720
Cfr9I CCCGGG 1 cut(s) 452
CseI GACGC 3 cut(s) 574, 1211, 1973
Csp6I GTAC 6 cut(s) 148, 463, 1126, 1723, 1746, 3327
CviQI GTAC 6 cut(s) 148, 463, 1126, 1723, 1746, 3327
DdeI CTNAG 6 cut(s) 1226, 1279, 2229, 2418, 2977, 3093
DraIII CACNNNGTG 2 cut(s) 168, 1525
DriI GACNNNNNGTC 1 cut(s) 649
EaeI YGGCCR 4 cut(s) 491, 504, 1753, 2436
EagI CGGCCG 1 cut(s) 504
Eam1104I CTCTTC 5 cut(s) 660, 714, 833, 2531, 2887
Eam1105I GACNNNNNGTC 1 cut(s) 649
EarI CTCTTC 5 cut(s) 660, 714, 833, 2531, 2887
EciI GGCGGA 3 cut(s) 1718, 2738, 3302
Ecl136II GAGCTC 1 cut(s) 1686
EclXI CGGCCG 1 cut(s) 504
Eco130I CCWWGG 4 cut(s) 1497, 2439, 3099, 3264
Eco147I AGGCCT 3 cut(s) 1639, 3098, 3125
Eco24I GRGCYC 1 cut(s) 1688
Eco31I GGTCTC 3 cut(s) 134, 369, 1052
Eco47I GGWCC 3 cut(s) 5, 576, 720
Eco52I CGGCCG 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 1686
Eco57I CTGAAG 6 cut(s) 368, 369, 377, 1971, 2020, 2505
Eco88I CYCGRG 5 cut(s) 110, 341, 452, 478, 3171
Eco91I GGTNACC 2 cut(s) 1874, 2699
EcoICRI GAGCTC 1 cut(s) 1686
EcoO109I RGGNCCY 3 cut(s) 5, 576, 720
EcoO65I GGTNACC 2 cut(s) 1874, 2699
EcoRI GAATTC 2 cut(s) 632, 1106
EcoRII CCWGG 2 cut(s) 181, 954
EcoT14I CCWWGG 4 cut(s) 1497, 2439, 3099, 3264
EcoT38I GRGCYC 1 cut(s) 1688
ErhI CCWWGG 4 cut(s) 1497, 2439, 3099, 3264
Esp3I CGTCTC 3 cut(s) 657, 1229, 2150
FalI AAGNNNNNCTT 2 cut(s) 1155, 1187
FaqI GGGAC 5 cut(s) 16, 18, 207, 399, 733
FauI CCCGC 4 cut(s) 266, 327, 1237, 3368
FauNDI CATATG 2 cut(s) 808, 2275
FblI GTMKAC 3 cut(s) 733, 1493, 2537
FriOI GRGCYC 1 cut(s) 1688
FspBI CTAG 7 cut(s) 996, 1017, 1196, 1509, 2189, 2646, 3524
FspI TGCGCA 1 cut(s) 2409
GlaI GCGC 4 cut(s) 137, 339, 931, 2409
GsuI CTGGAG 2 cut(s) 1490, 3180
HaeII RGCGCY 1 cut(s) 341
HapII CCGG 5 cut(s) 62, 325, 393, 453, 1872
HgaI GACGC 3 cut(s) 574, 1211, 1973
HhaI GCGC 4 cut(s) 138, 340, 932, 2410
Hin1I GRCGYC 2 cut(s) 500, 1965
Hin6I GCGC 4 cut(s) 136, 338, 930, 2408
HinP1I GCGC 4 cut(s) 136, 338, 930, 2408
HincII GTYRAC 2 cut(s) 734, 1494
HindII GTYRAC 2 cut(s) 734, 1494
HindIII AAGCTT 1 cut(s) 2032
HpaII CCGG 5 cut(s) 62, 325, 393, 453, 1872
Hpy166II GTNNAC 8 cut(s) 298, 465, 734, 1494, 1864, 1885, 2353, 2538
Hpy8I GTNNAC 8 cut(s) 298, 465, 734, 1494, 1864, 1885, 2353, 2538
Hpy99I CGWCG 5 cut(s) 322, 373, 505, 625, 738
HpyCH4III ACNGT 8 cut(s) 125, 625, 991, 1750, 2161, 2357, 2449, 3432
HpyCH4IV ACGT 4 cut(s) 146, 500, 1557, 1979
HpyF3I CTNAG 6 cut(s) 1226, 1279, 2229, 2418, 2977, 3093
HpySE526I ACGT 4 cut(s) 146, 500, 1557, 1979
Hsp92I GRCGYC 2 cut(s) 500, 1965
HspAI GCGC 4 cut(s) 136, 338, 930, 2408
KflI GGGWCCC 2 cut(s) 5, 720
Kpn2I TCCGGA 1 cut(s) 61
KpnI GGTACC 1 cut(s) 1726
LweI GCATC 5 cut(s) 126, 342, 955, 2561, 2919
MaeI CTAG 7 cut(s) 996, 1017, 1196, 1509, 2189, 2646, 3524
MaeII ACGT 4 cut(s) 146, 500, 1557, 1979
MbiI CCGCTC 2 cut(s) 273, 1244
MflI RGATCY 5 cut(s) 153, 1797, 2500, 2521, 3445
MhlI GDGCHC 1 cut(s) 1688
MlsI TGGCCA 2 cut(s) 1755, 2438
MluNI TGGCCA 2 cut(s) 1755, 2438
MlyI GAGTC 7 cut(s) 38, 1109, 2145, 2216, 2609, 2875, 2963
MmeI TCCRAC 7 cut(s) 102, 243, 1230, 1359, 1370, 2496, 3443
Mox20I TGGCCA 2 cut(s) 1755, 2438
MroI TCCGGA 1 cut(s) 61
MroXI GAANNNNTTC 4 cut(s) 1024, 1083, 2196, 2673
MscI TGGCCA 2 cut(s) 1755, 2438
MseI TTAA 5 cut(s) 599, 752, 2306, 2330, 3224
MslI CAYNNNNRTG 6 cut(s) 1395, 1952, 2294, 2682, 2874, 3480
Msp20I TGGCCA 2 cut(s) 1755, 2438
MspA1I CMGCKG 3 cut(s) 2072, 2417, 3296
MspI CCGG 5 cut(s) 62, 325, 393, 453, 1872
MspR9I CCNGG 5 cut(s) 183, 326, 453, 454, 956
Mva1269I GAATGC 1 cut(s) 3137
MvaI CCWGG 2 cut(s) 183, 956
MvnI CGCG 4 cut(s) 27, 138, 1881, 2081
NciI CCSGG 3 cut(s) 326, 453, 454
NdeI CATATG 2 cut(s) 808, 2275
NheI GCTAGC 2 cut(s) 2645, 3523
NmeAIII GCCGAG 2 cut(s) 510, 532
NsbI TGCGCA 1 cut(s) 2409
NspI RCATGY 2 cut(s) 2933, 3366
NspV TTCGAA 3 cut(s) 2463, 2564, 3132
OliI CACNNNNGTG 1 cut(s) 1952
PaeR7I CTCGAG 2 cut(s) 110, 478
PagI TCATGA 3 cut(s) 513, 570, 2320
PceI AGGCCT 3 cut(s) 1639, 3098, 3125
PciI ACATGT 1 cut(s) 2929
PcsI WCGNNNNNNNCGW 3 cut(s) 108, 117, 1119
PctI GAATGC 1 cut(s) 3137
PdmI GAANNNNTTC 4 cut(s) 1024, 1083, 2196, 2673
PfeI GAWTC 6 cut(s) 783, 943, 2317, 2380, 2820, 3055
Pfl23II CGTACG 1 cut(s) 147
PflFI GACNNNGTC 2 cut(s) 89, 1489
PflMI CCANNNNNTGG 1 cut(s) 2212
PleI GAGTC 7 cut(s) 37, 1109, 2144, 2216, 2609, 2874, 2963
PpsI GAGTC 7 cut(s) 37, 1109, 2144, 2216, 2609, 2874, 2963
Ppu21I YACGTR 2 cut(s) 147, 1558
PpuMI RGGWCCY 3 cut(s) 5, 576, 720
PscI ACATGT 1 cut(s) 2929
Psp124BI GAGCTC 1 cut(s) 1688
Psp5II RGGWCCY 3 cut(s) 5, 576, 720
Psp6I CCWGG 2 cut(s) 181, 954
PspEI GGTNACC 2 cut(s) 1874, 2699
PspGI CCWGG 2 cut(s) 181, 954
PspLI CGTACG 1 cut(s) 147
PspPI GGNCC 4 cut(s) 5, 96, 576, 720
PspPPI RGGWCCY 3 cut(s) 5, 576, 720
PspXI VCTCGAGB 2 cut(s) 110, 478
PstI CTGCAG 1 cut(s) 2071
PsuI RGATCY 5 cut(s) 153, 1797, 2500, 2521, 3445
PsyI GACNNNGTC 2 cut(s) 89, 1489
PvuII CAGCTG 2 cut(s) 2072, 2417
RsaI GTAC 6 cut(s) 149, 464, 1127, 1724, 1747, 3328
RsaNI GTAC 6 cut(s) 148, 463, 1126, 1723, 1746, 3327
RseI CAYNNNNRTG 6 cut(s) 1395, 1952, 2294, 2682, 2874, 3480
SacI GAGCTC 1 cut(s) 1688
SalI GTCGAC 2 cut(s) 732, 1492
SaqAI TTAA 5 cut(s) 599, 752, 2306, 2330, 3224
Sau96I GGNCC 4 cut(s) 5, 96, 576, 720
SchI GAGTC 7 cut(s) 38, 1109, 2145, 2216, 2609, 2875, 2963
ScrFI CCNGG 5 cut(s) 183, 326, 453, 454, 956
SduI GDGCHC 1 cut(s) 1688
SfaNI GCATC 5 cut(s) 126, 342, 955, 2561, 2919
SfcI CTRYAG 2 cut(s) 1308, 2067
SfiI GGCCNNNNNGGCC 1 cut(s) 490
Sfr274I CTCGAG 2 cut(s) 110, 478
SfuI TTCGAA 3 cut(s) 2463, 2564, 3132
SinI GGWCC 3 cut(s) 5, 576, 720
SlaI CTCGAG 2 cut(s) 110, 478
SmaI CCCGGG 1 cut(s) 454
SmiMI CAYNNNNRTG 6 cut(s) 1395, 1952, 2294, 2682, 2874, 3480
SmlI CTYRAG 5 cut(s) 110, 478, 1995, 2048, 2627
SmoI CTYRAG 5 cut(s) 110, 478, 1995, 2048, 2627
SpeI ACTAGT 1 cut(s) 995
SseBI AGGCCT 3 cut(s) 1639, 3098, 3125
SspI AATATT 1 cut(s) 3459
SspMI CTAG 7 cut(s) 996, 1017, 1196, 1509, 2189, 2646, 3524
SstI GAGCTC 1 cut(s) 1688
StuI AGGCCT 3 cut(s) 1639, 3098, 3125
StyD4I CCNGG 5 cut(s) 181, 324, 451, 452, 954
StyI CCWWGG 4 cut(s) 1497, 2439, 3099, 3264
TaaI ACNGT 8 cut(s) 125, 625, 991, 1750, 2161, 2357, 2449, 3432
TaiI ACGT 4 cut(s) 149, 503, 1560, 1982
TaqII GACCGA 1 cut(s) 162
TatI WGTACW 1 cut(s) 3326
TauI GCSGC 5 cut(s) 27, 475, 493, 1147, 2084
TfiI GAWTC 6 cut(s) 783, 943, 2317, 2380, 2820, 3055
Tru1I TTAA 5 cut(s) 599, 752, 2306, 2330, 3224
Tru9I TTAA 5 cut(s) 599, 752, 2306, 2330, 3224
TscAI CASTG 5 cut(s) 1367, 1954, 2097, 2360, 2907
TspGWI ACGGA 7 cut(s) 211, 357, 444, 551, 684, 1353, 2148
TspMI CCCGGG 1 cut(s) 452
TspRI CASTG 5 cut(s) 1367, 1954, 2097, 2360, 2907
Tth111I GACNNNGTC 2 cut(s) 89, 1489
Van91I CCANNNNNTGG 1 cut(s) 2212
VpaK11BI GGWCC 3 cut(s) 5, 576, 720
XapI RAATTY 5 cut(s) 632, 1023, 1106, 2466, 2894
XbaI TCTAGA 1 cut(s) 1016
XceI RCATGY 2 cut(s) 2933, 3366
XcmI CCANNNNNNNNNTGG 3 cut(s) 1039, 2446, 3271
XhoI CTCGAG 2 cut(s) 110, 478
XmaI CCCGGG 1 cut(s) 452
XmiI GTMKAC 3 cut(s) 733, 1493, 2537
XmnI GAANNNNTTC 4 cut(s) 1024, 1083, 2196, 2673
XspI CTAG 7 cut(s) 996, 1017, 1196, 1509, 2189, 2646, 3524
ZraI GACGTC 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.