Rw4G004370

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014182
Physical Location & Seq
Reverse (-)
8678121 .. 8682155
4035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004370.1

Sequence Viewer

Length: 2985 bp
ATGGGCCACCATGCTCGCCGTGACTCTCTCTGCAGCTCCCTACTAGCTCCTAGGCCCGACTACCACGACTCCGACCCCGACCTCGTCTGGCCCTTCGGCAAGCTCGATGGCATAGACCGTGATGATATCCGCGAGACTGCCTACGAGATCTTCTTTACCGCCTGCCGTTCTTCCCCGGGTTTCGGGGGCCGAAACGCCCTCGTGTTTTATTCCAACCACGAGAACGGTAGCGGGGAAGGCGGTGGGTCTGGGGCGGGGCCGAAGCCAAGCGGGGTGGTCACCACCCCGATCAGCCGGATAAAGAGGGCTCTTGGGCTTAAGATGCTCAAGCGCTCTCCGTCGAGGAGGATGAGTAGTGGTGGACGTTCAAGCCCGTCGTCTCCGAACGGGGCTTCAACTATGGAAAGGTCTCCTTCAACTATGGAAAGGGGTTCAGGAATGTCGTTCACAGCGCCGCCATCGAGGCCGAGGAGGCCAATGACCTCGGCGGAGATCATGAGGCAGCAGATGAGAGTCACCGAGCAGAGCGACGGCCGCCTTAGGAAAACCCTGATGAGAACCCTCGTAGGCCAAATGGGTAGGAGAGCAGAGACGATAATCCTCCCACTGGAGCTTCTCCGCCACCTAAAACCCTCCGAATTCAACGACTCCCACGAATACCACCACTGGCAAAAGCGGCAGCTCAAAATCCTCGAAGCCGGTCTCCTCCACTACCCCTCAATCCCACTCGACAGGTCCAACACCTTCGCCATGCGTCTTCGTGAAATCATCAGATCAGTCGACACGAAACCCATCGACACTGGCAAAAACTCCGATACAATGCGAACCCTATGCAACTCAGTTGTCTCATTATCATGGCGGAGCTCCAACGGAACCCCAACCGACGTTTGCCACTGGGCCGATGGCTTCCCCCTCAACATCCACCTCTATGTATCTCTCCTTCAATCTGTCTTCGATATCAGAGACGAGACTTTAGTCCTCGACGAGGTGGACGAGCTTCTCGAGCTGATGAAGAAGACTTGGTCCACTTTGGGGATCACTAGGCCGATTCACAATGTTTGTTTCACGTGGGTTTTGTTCCAGCAGTATGTTTCGACGGCGCAGATTGAGCCGGACTTGTTGTGTGCGGCGCACGCAATGTTGGCGGAGGTGGCCAACAATGCCAAGAGGCCTGACCGTGAGGCTATATATGCGTGGGCGGAGAAGAAACTGCTTCGGTATCATGAGTATTTTCAGAGAGGGACAGTTGGACAGATTGAAAACCTTCTTCCTCTAGCCTTGTCATCATCCAAGATTTTGGGTGAAGATGTTTCCATCACAGATGGGGCAGGGGATGGGAAAGGTGATACTACGTTGGTGGATAACTCCGGTGATCGTATCATAGAAGCTGGGAATGTCACCGAGGTGAAAGAGGAGGCAGTGACTGAAGCTTTACTTCAATTGGCCAAAGACACAGAAGATTTAGCCTGTAAAGAGAGGGAGAGCTTCAGTCCCATACTAAAGAGATGGCACACAACAGCGGCGGGGATTGCAGCCGTGACGCTGCACAACTGTTATGGAGCTGTCTTGAAGCAATACTTGAATGGAGTGTCCACACTTACAGCAGACACAGTTGAGATACTGCAGAGGGCAGGAAAGCTAGAGAAGGTTCTGCTCCAAATGGTGGTGGAGGACTCTGCTGAATGCGAAGATGGGGGGAAAGCGATTGTGAGAGAGATGGTTCCATATGAAGTTGATAGCATCATAGTGAACCTTTTGAAAAGATGGATTTATGAGAGAATGAAGAGAGGGAAAGAGTGTGTCAACAGAGCAAAAGAGAGCGAGACATGGAATCCAAAGTCTAAATCAGAGCCATATGCACAATCAGCTGAGGAGCTAATGAAATTGGCAAAGGAAATTGTGGACGAGTTCTTTGAAATCCCAATTGGAATTACAGAAGATTTAGTTCAAGATCTTGCTGATGGTATGGAACATCTCTTCAAGGAGTACACCGCATTTGTTGCTTCTTGCGGTTCCAAACAGAGTTATATTCCTACACTTCCTCCTTTGACAAGATGCAACCGAGACTCAAAGATCCTCAAGCTGTGGAAAAAGGCTAGCCCTTGTAGTATTGGAGCTGATGATTTCCACCCAAATGGAACAAATGAAGGTCACCATCCTCGACCTTCAACAAGCCGAGGAACACAACGCCTATATATTCGCCTCAACACCTTGCACTATCTTCTCTCCCACCTTCATTCTCTTGACAAAAACCTTTCTCTCTCCCCTCGAATCATTCCTTCAACACCACGCAGCCGCTATGCCAACAACCGGAGGGCCAACAATTCCTCATACTTCGAAATCACCCATGCATCAATCCAAGCAGCATGCCAACATGTCTCAGCAGTAGCTGCTTATCGCTTAATCTTCCTCGACTCCAACTCTGTCTTCTATGAAAGTCTCTATGTTGGTGATGTAGCCAATGCAAGAATTAGACCTGCATTGCGAATTCTCAAGCAGAATCTTACTCTTCTGGGTGCAATTCTCACTGATAGAGCACAGGCATTGGCAATAAGAGAAGTAATGAGGACATCTTTTGAGGCATTCCTTATGGTTTTGGTTGCTGGGGGAAGCTCCCGGGTGTTTTATCGGAGTGATCATGAGATGATCGAGGAGGATTTTGATAGCCTGAAACGCGTATTTTGCTCTCATGGAGAAGGATTAATAGCCAAGGATGTGGTGGAACATGAGGCGGAGACAGCGGAAGGGGTGATTGATTTGATGGGCCAGTGCACTGAACAACTTATGGAAGATTTCAGCATTGTGACTTGTGAAACAAGTGGGATAGGAGTTGTTGGTTCGGGGCAAAGGCTGCCGATGCCTCCAACAACAGGAAGGTGGAATAGATCAGATCCAAATACTATATTGAGGGTCTTGTGCCACAGGAATGACAAAGCAGCAAACCAATTCTTAAAGAGAACATTCCAGTTAGCAAAGAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

994

Amino Acids

111.17

Weight (kDa)

7.59

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 582 - 994 9e-185 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04470 AT2G33420
fragaria_vesca FvH4_4g04280 FvH4_4g04280
malus_domestica MD13G1219000.v1.1 MD16G1224300.v1.1
prunus_persica Prupe.1G045700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394731
rosa_laevigata RLG00000009667
rosa_multiflora Rmu_sc0005356.1_g000011
rosa_roxburghii Rroxscaffold_5G00339750
rosa_rugosa Rorug03G0362100.1
rosa_samantha Rh4AG054800 Rh4BG052500 Rh4CG059300 Rh4DG051100
rosa_wichuraiana Rw4G004370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2485
AccB7I CCANNNNNTGG 1 cut(s) 1663
AccI GTMKAC 1 cut(s) 780
AccII CGCG 2 cut(s) 132, 2676
AclWI GGATC 3 cut(s) 1043, 2068, 2885
AcoI YGGCCR 3 cut(s) 532, 1152, 1443
AcsI RAATTY 2 cut(s) 638, 2487
AcuI CTGAAG 2 cut(s) 1446, 1471
AcvI CACGTG 1 cut(s) 1068
AfaI GTAC 1 cut(s) 1988
AfeI AGCGCT 1 cut(s) 332
AfiI CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1663, 2310, 2870
AflII CTTAAG 1 cut(s) 317
AflIII ACRYGT 2 cut(s) 2374, 2674
AjuI GAANNNNNNNTTGG 4 cut(s) 1908, 1940, 2411, 2443
AleI CACNNNNGTG 1 cut(s) 1403
Alw21I GWGCWC 3 cut(s) 866, 2539, 2774
Alw44I GTGCAC 1 cut(s) 2770
AlwI GGATC 3 cut(s) 1043, 2068, 2885
AlwNI CAGNNNCTG 2 cut(s) 1424, 2390
Ama87I CYCGRG 3 cut(s) 175, 1001, 2616
Aor51HI AGCGCT 1 cut(s) 332
ApaLI GTGCAC 1 cut(s) 2770
ApoI RAATTY 2 cut(s) 638, 2487
ArsI GACNNNNNNTTYG 2 cut(s) 1895, 1927
AseI ATTAAT 1 cut(s) 2702
Asp700I GAANNNNTTC 1 cut(s) 1263
AspA2I CCTAGG 1 cut(s) 50
AspLEI GCGC 4 cut(s) 333, 454, 1102, 1132
AsuC2I CCSGG 4 cut(s) 176, 177, 2617, 2618
AsuII TTCGAA 1 cut(s) 2337
AsuNHI GCTAGC 1 cut(s) 2096
AvaI CYCGRG 3 cut(s) 175, 1001, 2616
AvaII GGWCC 2 cut(s) 735, 1023
AvrII CCTAGG 1 cut(s) 50
AxyI CCTNAGG 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 2774
BalI TGGCCA 2 cut(s) 1154, 1445
BanII GRGCYC 2 cut(s) 310, 866
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BauI CACGAG 2 cut(s) 200, 218
BbrPI CACGTG 1 cut(s) 1068
BbsI GAAGAC 4 cut(s) 749, 943, 1022, 2419
Bbv12I GWGCWC 3 cut(s) 866, 2539, 2774
BbvCI CCTCAGC 1 cut(s) 1869
BceAI ACGGC 5 cut(s) 3, 150, 547, 1113, 1520
BcgI CGANNNNNNTGC 2 cut(s) 813, 847
BclI TGATCA 1 cut(s) 2635
BcnI CCSGG 4 cut(s) 176, 177, 2617, 2618
BfaI CTAG 6 cut(s) 44, 51, 1041, 1274, 1640, 2097
BfmI CTRYAG 2 cut(s) 31, 1622
BfoI RGCGCY 2 cut(s) 334, 455
BfrI CTTAAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 2485
BglI GCCNNNNNGGC 2 cut(s) 463, 472
BglII AGATCT 2 cut(s) 147, 1951
BlnI CCTAGG 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 176, 177, 2617, 2618
Bme18I GGWCC 2 cut(s) 735, 1023
BmeT110I CYCGRG 3 cut(s) 175, 1001, 2616
BmiI GGNNCC 5 cut(s) 188, 258, 874, 1722, 2014
BmrFI CCNGG 4 cut(s) 176, 177, 2617, 2618
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 5 cut(s) 312, 1749, 2045, 2360, 2847
BmtI GCTAGC 1 cut(s) 2100
BmuI ACTGGG 1 cut(s) 904
BoxI GACNNNNGTC 1 cut(s) 974
BpiI GAAGAC 4 cut(s) 749, 943, 1022, 2419
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 1869
Bpu14I TTCGAA 1 cut(s) 2337
BpuEI CTTGAG 3 cut(s) 311, 2063, 2477
BpuMI CCSGG 4 cut(s) 176, 177, 2617, 2618
BsaAI YACGTR 1 cut(s) 1068
BsaI GGTCTC 2 cut(s) 414, 707
BsaJI CCNNGG 9 cut(s) 50, 174, 175, 467, 483, 1401, 2176, 2616, 2709
BsaWI WCCGGW 2 cut(s) 1367, 2310
BsaXI ACNNNNNCTCC 6 cut(s) 53, 83, 337, 367, 2026, 2056
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1663, 2310, 2870
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 6 cut(s) 612, 671, 805, 899, 2767, 2965
Bse21I CCTNAGG 1 cut(s) 539
Bse3DI GCAATG 2 cut(s) 1143, 2480
BseDI CCNNGG 9 cut(s) 50, 174, 175, 467, 483, 1401, 2176, 2616, 2709
BseGI GGATG 6 cut(s) 354, 918, 1286, 1339, 2155, 2719
BseLI CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1663, 2310, 2870
BseMI GCAATG 2 cut(s) 1143, 2480
BseMII CTCAG 3 cut(s) 852, 1860, 2394
BseNI ACTGG 6 cut(s) 612, 671, 805, 899, 2767, 2965
BseRI GAGGAG 6 cut(s) 358, 484, 695, 1427, 1886, 2666
BseSI GKGCMC 1 cut(s) 2774
BseX3I CGGCCG 1 cut(s) 532
BseYI CCCAGC 2 cut(s) 1388, 2603
BsgI GTGCAG 1 cut(s) 1529
Bsh1236I CGCG 2 cut(s) 132, 2676
Bsh1285I CGRYCG 1 cut(s) 535
BsiEI CGRYCG 1 cut(s) 535
BsiHKAI GWGCWC 3 cut(s) 866, 2539, 2774
BsiHKCI CYCGRG 3 cut(s) 175, 1001, 2616
BsiSI CCGG 7 cut(s) 176, 295, 699, 1112, 1368, 2311, 2617
BslFI GGGAC 2 cut(s) 1255, 1476
BslI CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1663, 2310, 2870
BsmBI CGTCTC 3 cut(s) 384, 584, 957
BsmFI GGGAC 2 cut(s) 1255, 1476
BsmI GAATGC 2 cut(s) 1688, 2582
Bso31I GGTCTC 2 cut(s) 414, 707
BsoBI CYCGRG 3 cut(s) 175, 1001, 2616
Bsp119I TTCGAA 1 cut(s) 2337
Bsp1286I GDGCHC 4 cut(s) 310, 866, 2539, 2774
BspCNI CTCAG 3 cut(s) 851, 1861, 2393
BspFNI CGCG 2 cut(s) 132, 2676
BspHI TCATGA 3 cut(s) 495, 1222, 2638
BspLI GGNNCC 5 cut(s) 188, 258, 874, 1722, 2014
BspMAI CTGCAG 2 cut(s) 35, 1626
BspMI ACCTGC 1 cut(s) 2485
BspOI GCTAGC 1 cut(s) 2100
BspPI GGATC 3 cut(s) 1043, 2068, 2885
BspT104I TTCGAA 1 cut(s) 2337
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 2 cut(s) 414, 707
BsrDI GCAATG 2 cut(s) 1143, 2480
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 6 cut(s) 612, 671, 805, 899, 2767, 2965
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 9 cut(s) 50, 174, 175, 467, 483, 1401, 2176, 2616, 2709
BssSI CACGAG 2 cut(s) 200, 218
BssT1I CCWWGG 2 cut(s) 50, 2709
Bst2BI CACGAG 2 cut(s) 200, 218
Bst4CI ACNGT 6 cut(s) 119, 227, 1178, 1246, 1553, 1612
Bst6I CTCTTC 3 cut(s) 1778, 1982, 2514
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 3 cut(s) 2000, 2390, 2851
BstBAI YACGTR 1 cut(s) 1068
BstBI TTCGAA 1 cut(s) 2337
BstC8I GCNNGC 6 cut(s) 16, 101, 163, 1134, 2098, 2368
BstDEI CTNAG 4 cut(s) 539, 838, 1869, 2380
BstEII GGTNACC 2 cut(s) 277, 2150
BstENI CCTNNNNNAGG 1 cut(s) 983
BstF5I GGATG 6 cut(s) 354, 918, 1286, 1339, 2155, 2719
BstFNI CGCG 2 cut(s) 132, 2676
BstH2I RGCGCY 2 cut(s) 334, 455
BstHHI GCGC 4 cut(s) 333, 454, 1102, 1132
BstMCI CGRYCG 1 cut(s) 535
BstNSI RCATGY 2 cut(s) 2370, 2378
BstPAI GACNNNNGTC 1 cut(s) 974
BstPI GGTNACC 2 cut(s) 277, 2150
BstSCI CCNGG 4 cut(s) 174, 175, 2615, 2616
BstSFI CTRYAG 2 cut(s) 31, 1622
BstSLI GKGCMC 1 cut(s) 2774
BstUI CGCG 2 cut(s) 132, 2676
BstV2I GAAGAC 4 cut(s) 749, 943, 1022, 2419
BstX2I RGATCY 4 cut(s) 147, 1951, 2073, 2890
BstXI CCANNNNNNTGG 3 cut(s) 1297, 2135, 2716
BstYI RGATCY 4 cut(s) 147, 1951, 2073, 2890
BstZI CGGCCG 1 cut(s) 532
Bsu36I CCTNAGG 1 cut(s) 539
BtsCI GGATG 6 cut(s) 354, 918, 1286, 1339, 2155, 2719
BtsI GCAGTG 1 cut(s) 1425
BtsIMutI CAGTG 8 cut(s) 605, 664, 798, 892, 1425, 2526, 2772, 2774
BveI ACCTGC 1 cut(s) 2485
Cac8I GCNNGC 6 cut(s) 16, 101, 163, 1134, 2098, 2368
CaiI CAGNNNCTG 2 cut(s) 1424, 2390
CciI TCATGA 3 cut(s) 495, 1222, 2638
CfoI GCGC 4 cut(s) 333, 454, 1102, 1132
Cfr10I RCCGGY 1 cut(s) 698
Cfr9I CCCGGG 2 cut(s) 175, 2616
CseI GACGC 2 cut(s) 743, 1549
Csp6I GTAC 1 cut(s) 1987
CspCI CAANNNNNGTGG 2 cut(s) 255, 290
CviQI GTAC 1 cut(s) 1987
DdeI CTNAG 4 cut(s) 539, 838, 1869, 2380
EaeI YGGCCR 3 cut(s) 532, 1152, 1443
EagI CGGCCG 1 cut(s) 532
Eam1104I CTCTTC 3 cut(s) 1778, 1982, 2514
EarI CTCTTC 3 cut(s) 1778, 1982, 2514
EciI GGCGGA 6 cut(s) 503, 608, 874, 1160, 1214, 2747
Ecl136II GAGCTC 1 cut(s) 864
EclXI CGGCCG 1 cut(s) 532
Eco130I CCWWGG 2 cut(s) 50, 2709
Eco147I AGGCCT 1 cut(s) 1171
Eco24I GRGCYC 2 cut(s) 310, 866
Eco31I GGTCTC 2 cut(s) 414, 707
Eco32I GATATC 2 cut(s) 127, 958
Eco47I GGWCC 2 cut(s) 735, 1023
Eco47III AGCGCT 1 cut(s) 332
Eco52I CGGCCG 1 cut(s) 532
Eco53kI GAGCTC 1 cut(s) 864
Eco57I CTGAAG 2 cut(s) 1446, 1471
Eco72I CACGTG 1 cut(s) 1068
Eco81I CCTNAGG 1 cut(s) 539
Eco88I CYCGRG 3 cut(s) 175, 1001, 2616
Eco91I GGTNACC 2 cut(s) 277, 2150
EcoICRI GAGCTC 1 cut(s) 864
EcoNI CCTNNNNNAGG 1 cut(s) 983
EcoO65I GGTNACC 2 cut(s) 277, 2150
EcoRI GAATTC 2 cut(s) 638, 2487
EcoRV GATATC 2 cut(s) 127, 958
EcoT14I CCWWGG 2 cut(s) 50, 2709
EcoT22I ATGCAT 1 cut(s) 2353
EcoT38I GRGCYC 2 cut(s) 310, 866
ErhI CCWWGG 2 cut(s) 50, 2709
Esp3I CGTCTC 3 cut(s) 384, 584, 957
FalI AAGNNNNNCTT 6 cut(s) 397, 429, 1419, 1451, 1562, 1594
FaqI GGGAC 2 cut(s) 1255, 1476
FauI CCCGC 4 cut(s) 224, 247, 263, 1516
FauNDI CATATG 2 cut(s) 1726, 1855
FbaI TGATCA 1 cut(s) 2635
FblI GTMKAC 1 cut(s) 780
FokI GGATG 6 cut(s) 361, 905, 1273, 1346, 2142, 2726
FriOI GRGCYC 2 cut(s) 310, 866
FspBI CTAG 6 cut(s) 44, 51, 1041, 1274, 1640, 2097
GlaI GCGC 4 cut(s) 332, 453, 1101, 1131
GsaI CCCAGC 2 cut(s) 1392, 2607
GsuI CTGGAG 1 cut(s) 629
HaeII RGCGCY 2 cut(s) 334, 455
HapII CCGG 7 cut(s) 176, 295, 699, 1112, 1368, 2311, 2617
HgaI GACGC 2 cut(s) 743, 1549
HhaI GCGC 4 cut(s) 333, 454, 1102, 1132
Hin6I GCGC 4 cut(s) 331, 452, 1100, 1130
HinP1I GCGC 4 cut(s) 331, 452, 1100, 1130
HincII GTYRAC 2 cut(s) 781, 1804
HindII GTYRAC 2 cut(s) 781, 1804
HindIII AAGCTT 1 cut(s) 1428
HpaII CCGG 7 cut(s) 176, 295, 699, 1112, 1368, 2311, 2617
Hpy188III TCNNGA 9 cut(s) 435, 496, 761, 1001, 1223, 1567, 1949, 2243, 2639
Hpy99I CGWCG 6 cut(s) 343, 379, 533, 887, 986, 1099
HpyCH4III ACNGT 6 cut(s) 119, 227, 1178, 1246, 1553, 1612
HpyCH4IV ACGT 4 cut(s) 364, 885, 1067, 1352
HpyF3I CTNAG 4 cut(s) 539, 838, 1869, 2380
HpySE526I ACGT 4 cut(s) 364, 885, 1067, 1352
HspAI GCGC 4 cut(s) 331, 452, 1100, 1130
Ksp22I TGATCA 1 cut(s) 2635
LweI GCATC 5 cut(s) 312, 1749, 2045, 2360, 2847
MaeI CTAG 6 cut(s) 44, 51, 1041, 1274, 1640, 2097
MaeII ACGT 4 cut(s) 364, 885, 1067, 1352
MaeIII GTNAC 8 cut(s) 20, 277, 514, 1396, 1420, 1537, 2150, 2803
MfeI CAATTG 2 cut(s) 1439, 1923
MflI RGATCY 4 cut(s) 147, 1951, 2073, 2890
MhlI GDGCHC 4 cut(s) 310, 866, 2539, 2774
MlsI TGGCCA 2 cut(s) 1154, 1445
MluI ACGCGT 1 cut(s) 2674
MluNI TGGCCA 2 cut(s) 1154, 1445
MlyI GAGTC 7 cut(s) 17, 62, 522, 641, 1667, 2060, 2408
MmeI TCCRAC 7 cut(s) 96, 237, 762, 891, 1228, 2442, 2888
Mox20I TGGCCA 2 cut(s) 1154, 1445
Mph1103I ATGCAT 1 cut(s) 2353
MroXI GAANNNNTTC 1 cut(s) 1263
MscI TGGCCA 2 cut(s) 1154, 1445
MseI TTAA 4 cut(s) 318, 2402, 2702, 2951
MslI CAYNNNNRTG 6 cut(s) 853, 927, 1403, 1745, 2133, 2925
Msp20I TGGCCA 2 cut(s) 1154, 1445
MspA1I CMGCKG 3 cut(s) 1520, 1868, 2741
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 7 cut(s) 176, 295, 699, 1112, 1368, 2311, 2617
MspR9I CCNGG 4 cut(s) 176, 177, 2617, 2618
MunI CAATTG 2 cut(s) 1439, 1923
Mva1269I GAATGC 2 cut(s) 1688, 2582
MvnI CGCG 2 cut(s) 132, 2676
NciI CCSGG 4 cut(s) 176, 177, 2617, 2618
NdeI CATATG 2 cut(s) 1726, 1855
NheI GCTAGC 1 cut(s) 2096
NlaIV GGNNCC 5 cut(s) 188, 258, 874, 1722, 2014
NmeAIII GCCGAG 3 cut(s) 464, 492, 2201
NmuCI GTSAC 8 cut(s) 20, 277, 514, 1396, 1420, 1537, 2150, 2803
NsiI ATGCAT 1 cut(s) 2353
NspI RCATGY 2 cut(s) 2370, 2378
NspV TTCGAA 1 cut(s) 2337
OliI CACNNNNGTG 1 cut(s) 1403
PaeI GCATGC 1 cut(s) 2370
PaeR7I CTCGAG 1 cut(s) 1001
PagI TCATGA 3 cut(s) 495, 1222, 2638
PceI AGGCCT 1 cut(s) 1171
PciI ACATGT 1 cut(s) 2374
PcsI WCGNNNNNNNCGW 4 cut(s) 102, 651, 990, 999
PctI GAATGC 2 cut(s) 1688, 2582
PdmI GAANNNNTTC 1 cut(s) 1263
PfeI GAWTC 4 cut(s) 1048, 1831, 2271, 2500
PflFI GACNNNGTC 2 cut(s) 83, 1021
PflMI CCANNNNNTGG 1 cut(s) 1663
PleI GAGTC 7 cut(s) 17, 62, 521, 641, 1667, 2060, 2408
PmaCI CACGTG 1 cut(s) 1068
PmlI CACGTG 1 cut(s) 1068
PpsI GAGTC 7 cut(s) 17, 62, 521, 641, 1667, 2060, 2408
Ppu21I YACGTR 1 cut(s) 1068
PscI ACATGT 1 cut(s) 2374
PshAI GACNNNNGTC 1 cut(s) 974
PshBI ATTAAT 1 cut(s) 2702
Psp124BI GAGCTC 1 cut(s) 866
PspCI CACGTG 1 cut(s) 1068
PspEI GGTNACC 2 cut(s) 277, 2150
PspFI CCCAGC 2 cut(s) 1388, 2603
PspN4I GGNNCC 5 cut(s) 188, 258, 874, 1722, 2014
PstI CTGCAG 2 cut(s) 35, 1626
PstNI CAGNNNCTG 2 cut(s) 1424, 2390
PsuI RGATCY 4 cut(s) 147, 1951, 2073, 2890
PsyI GACNNNGTC 2 cut(s) 83, 1021
PvuII CAGCTG 1 cut(s) 1868
RsaI GTAC 1 cut(s) 1988
RsaNI GTAC 1 cut(s) 1987
RseI CAYNNNNRTG 6 cut(s) 853, 927, 1403, 1745, 2133, 2925
SacI GAGCTC 1 cut(s) 866
SalI GTCGAC 1 cut(s) 779
SaqAI TTAA 4 cut(s) 318, 2402, 2702, 2951
SchI GAGTC 7 cut(s) 17, 62, 522, 641, 1667, 2060, 2408
ScrFI CCNGG 4 cut(s) 176, 177, 2617, 2618
SduI GDGCHC 4 cut(s) 310, 866, 2539, 2774
SfaNI GCATC 5 cut(s) 312, 1749, 2045, 2360, 2847
SfcI CTRYAG 2 cut(s) 31, 1622
SfiI GGCCNNNNNGGCC 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 1001
SfuI TTCGAA 1 cut(s) 2337
SinI GGWCC 2 cut(s) 735, 1023
SlaI CTCGAG 1 cut(s) 1001
SmaI CCCGGG 2 cut(s) 177, 2618
SmiMI CAYNNNNRTG 6 cut(s) 853, 927, 1403, 1745, 2133, 2925
SmlI CTYRAG 5 cut(s) 317, 326, 1001, 2078, 2492
SmoI CTYRAG 5 cut(s) 317, 326, 1001, 2078, 2492
SphI GCATGC 1 cut(s) 2370
SseBI AGGCCT 1 cut(s) 1171
SspMI CTAG 6 cut(s) 44, 51, 1041, 1274, 1640, 2097
SstI GAGCTC 1 cut(s) 866
StuI AGGCCT 1 cut(s) 1171
StyD4I CCNGG 4 cut(s) 174, 175, 2615, 2616
StyI CCWWGG 2 cut(s) 50, 2709
TaaI ACNGT 6 cut(s) 119, 227, 1178, 1246, 1553, 1612
TaiI ACGT 4 cut(s) 367, 888, 1070, 1355
TatI WGTACW 1 cut(s) 1986
TauI GCSGC 6 cut(s) 457, 537, 679, 1130, 1523, 2298
TfiI GAWTC 4 cut(s) 1048, 1831, 2271, 2500
Tru1I TTAA 4 cut(s) 318, 2402, 2702, 2951
Tru9I TTAA 4 cut(s) 318, 2402, 2702, 2951
TscAI CASTG 8 cut(s) 612, 671, 805, 899, 1425, 2533, 2774, 2779
TseFI GTSAC 8 cut(s) 20, 277, 514, 1396, 1420, 1537, 2150, 2803
Tsp45I GTSAC 8 cut(s) 20, 277, 514, 1396, 1420, 1537, 2150, 2803
TspDTI ATGAA 7 cut(s) 1025, 1743, 1796, 1895, 2160, 2225, 2448
TspGWI ACGGA 2 cut(s) 327, 885
TspMI CCCGGG 2 cut(s) 175, 2616
TspRI CASTG 8 cut(s) 612, 671, 805, 899, 1425, 2533, 2774, 2779
Tth111I GACNNNGTC 2 cut(s) 83, 1021
Van91I CCANNNNNTGG 1 cut(s) 1663
Vha464I CTTAAG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 2770
VpaK11BI GGWCC 2 cut(s) 735, 1023
VspI ATTAAT 1 cut(s) 2702
XagI CCTNNNNNAGG 1 cut(s) 983
XapI RAATTY 2 cut(s) 638, 2487
XceI RCATGY 2 cut(s) 2370, 2378
XcmI CCANNNNNNNNNTGG 2 cut(s) 899, 2716
XhoI CTCGAG 1 cut(s) 1001
XmaI CCCGGG 2 cut(s) 175, 2616
XmaJI CCTAGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 1 cut(s) 1263
XspI CTAG 6 cut(s) 44, 51, 1041, 1274, 1640, 2097
Zsp2I ATGCAT 1 cut(s) 2353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.