Rh4AG054800

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014182
Physical Location & Seq
Reverse (-)
11366635 .. 11370677
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG054800.1

Sequence Viewer

Length: 3066 bp
ATGGGCCACCATGCTCGCCGTGACTCTCTCTGCAGCTCCCTACTAGCTCCTAGGCCCGACTACCACGACTCCGACCCCGACCTCGTCTGGCCCTTCGGCAAGCTCGATGGCATAGACCGTGACGATATCCGCGAGACTGCCTACGAGATCTTCTTCACCGCCTGCCGTTCTTCCCCGGGTTTCGGGGGCCGAAACGCCCTCGTGTTTTATTCCAACCACGAGAACGGTAGCGGGGAAGGCGGTGGGTCTGGGGCGGGGCCGAAGCCAAGCGGGGTGGTCACCACCCCGATCAGCCGGATAAAGAGGGCTCTTGGGCTTAAGATGCTCAAGCGCTCTCCGTCGAGGAGGATGAGTAGTGGTGGACGTTCAAGCCCGTCGTCTCCGAACGGGGCTTCAACTATGGAAAGGTCTCCTTCAACTATGGAAAGGGGTTCAGGAATGTCGTTCACAGCGCCGCCATCGAGGCCGAGGAGGCCAATGACCTCGGCGGAGATCATGAGGCAGCAGATGAGAGTCACCGAGCAGAGCGACGGCCGCCTTAGGAAAACCCTGATGAGAACCCTCGTAGGCCAAATGGGTAGGAGAGCAGAGACGATAATCCTCCCACTGGAGCTTCTCCGCCACCTAAAACCCTCCGAATTCAACGACTCCCACGAATACCACCACTGGCAAAAGCGGCAGCTCAAAATCCTCGAAGCCGGTCTCCTCCACTACCCCTCAATCCCACTCGACAGGTCCAACACCTTCGCCATGCGTCTTCGTGAAATCATCAGATCAGTCGACACGAAACCCATCGACACTGGCAAAAACTCCGATACAATGCGAACCCTATGCAACTCAGTAGTCTCATTATCATGGCGGAGCTCCAACGGAACCCCAACCGACGTTTGCCACTGGGCCGATGGCTTCCCCCTCAACATCCACCTCTATGTATCTCTCCTTCAATCTGTCTTCGATATCAGAGACGAGACTTTAGTCCTCGACGAGGTGGACGAGCTTCTCGAGCTGATGAAGAAGACTTGGTCCACTTTGGGGATCACTAGGCCGATTCACAATGTTTGTTTCACGTGGGTTTTGTTCCAGCAGTATGTTTCGACGGCGCAGATTGAGCCGGACTTGTTGTGTGCGGCGCACGCAATGTTGGCGGAGGTGGCCAACAATGCCAAGAGGCCTGACCGTGAGGCTATATATGTTAAGATCTTGTCGGCGGTGTTGAGCTCCATGCAGGCGTGGGCGGAGAAGAAACTGCTTCGGTATCATGAGTATTTTCAGAGAGGGACAGTTGGACAGATTGAAAACCTTCTTCCTCTAGCCTTGTCATCATCCAAGATTTTGGGTGAAGATGTTTCCATCACAGATGGGGCAGGGGATGGGAAAGGTGATACTACGTTGGTGGATAACTCCGGTGATCGTGTTGATTACTACATTCGATCTTCCATGAAACAAGCTTTCGCAAAGATCATAGAAGCTGGGAATGTCACCGAGGTGAAAGAGGAGGCAGTGACTGAAGCTTTACTTCAATTGGCCAAAGACACAGAAGATTTAGCCTGTAAAGAGAGGGAGAGCTTCAGTCCCATACTAAAGAGATGGCACACAACAGCGGCGGGGATTGCAGCCGTGACGCTGCACAACTGTTATGGAGCTGTGTTGAAGCAATACTTGAATGGAGTGTCCACACTTACAGCAGACACAGTTGAGATACTGCAGAGGGCAGGAAAGCTAGAGAAGGTTCTGCTCCAAATGGTGGTGGAGGACTCTGCTGAATGCGAAGATGGGGGGAAAGCGATTGTGAGAGAGATGGTTCCATATGAAGTTGATAGCATCATAGTGAACCTTTTGAAAAGATGGATTTATGAGAGAATGAAGAGAGGGAAAGAGTGTGTCAACAGAGCAAAAGAGAGCGAGACATGGAATCCAAAGTCTAAATCAGAGCCATATGCACAATCAGCTGAGGAGCTAATGAAATTGGCAAAGGAAATTGTGGACGAGTTCTTTGAAATCCCAATTGGAATTACAGAAGATTTGGTTCAAGATCTTGCTGATGGTATGGAACATCTCTTCAAGGAGTACACCGCATTTGTTGCTTCTTGCGGTTCCAAACAGAGTTATATTCCTACACTTCCTCCTTTGACAAGATGCAACCGAGACTCAAAGATCCTCAAGCTGTGGAAAAAGGCTAGCCCTTGTAGTATTGGAGCTGATGATTTCCACCCAAATGGAACAAATGAAGGTCACCATCCTCGACCTTCAACAAGCCGAGGAACACAACGCCTATATATTCGCCTCAACACCTTGCACTATCTTCTCTCCCACCTTCATTCTCTTGACAAAAACCTTTCTCTCTCCCCTCGAATCATTCCTTCAACACCACGCAGCCGCTATGCCAACAACCGGAGGGCCAACAATTCCTCATACTTCGAAATCACCCATGCATCAATCCAAGCAGCATGCCAACATGTCTCAGAAGTAGCTGCTTATCGCTTAATCTTCCTCGACTCCAACTCTGTCTTCTATGAAAGTCTCTATGTTGGTGATGTAGCCAATGCAAGAATTAGACCTGCATTGCGAATTCTCAAGCAGAATCTTACTCTTCTGGGTGCAATTCTCACTGATAGAGCACAGGCATTGGCAATAAGAGAAGTAATGAGGACATCTTTTGAGGCATTCCTTATGGTTTTGGTTGCTGGGGGAAGCTCCCGGGTGTTTTATCGGAGTGATCATGAGATGATCGAGGAGGATTTTGATAGCCTGAAACGCGTATTTTGCTCTCATGGAGAAGGATTAATAGCCAAGGATGTGGTGGAACATGAGGCGGAGACAGCGGAAGGGGTGATTGATTTGATGGGCCAGTGCACTGAACAACTTATGGAAGATTTCAGCATTGTGACTTGTGAAACAAGTGGGATAGGAGTTGTTGGTTCGGGGCAAAGGCTGCCGATGCCTCCAACAACAGGAAGGTGGAATAGATCAGATCCAAATACTATATTGAGGGTCTTGTGCCACAGGAATGACAAAGCAGCAAACCAATTCTTAAAGAGAACATTCCAGTTAGCAAAGAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

1021

Amino Acids

114.27

Weight (kDa)

8.0

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 609 - 1021 1.2e-185 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04470 AT2G33420
fragaria_vesca FvH4_4g04280 FvH4_4g04280
malus_domestica MD13G1219000.v1.1 MD16G1224300.v1.1
prunus_persica Prupe.1G045700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394731
rosa_laevigata RLG00000009667
rosa_multiflora Rmu_sc0005356.1_g000011
rosa_roxburghii Rroxscaffold_5G00339750
rosa_rugosa Rorug03G0362100.1
rosa_samantha Rh4AG054800 Rh4BG052500 Rh4CG059300 Rh4DG051100
rosa_wichuraiana Rw4G004370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2566
AccB7I CCANNNNNTGG 1 cut(s) 1744
AccI GTMKAC 1 cut(s) 780
AccII CGCG 2 cut(s) 132, 2757
AclWI GGATC 3 cut(s) 1043, 2149, 2966
AcoI YGGCCR 3 cut(s) 532, 1152, 1524
AcsI RAATTY 2 cut(s) 638, 2568
AcuI CTGAAG 2 cut(s) 1527, 1552
AcvI CACGTG 1 cut(s) 1068
AfaI GTAC 1 cut(s) 2069
AfeI AGCGCT 1 cut(s) 332
AfiI CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1744, 2391, 2951
AflII CTTAAG 1 cut(s) 317
AflIII ACRYGT 2 cut(s) 2455, 2755
AjuI GAANNNNNNNTTGG 4 cut(s) 1989, 2021, 2492, 2524
AleI CACNNNNGTG 1 cut(s) 1484
Alw21I GWGCWC 4 cut(s) 866, 1220, 2620, 2855
Alw44I GTGCAC 1 cut(s) 2851
AlwI GGATC 3 cut(s) 1043, 2149, 2966
AlwNI CAGNNNCTG 1 cut(s) 1505
Ama87I CYCGRG 3 cut(s) 175, 1001, 2697
Aor51HI AGCGCT 1 cut(s) 332
ApaLI GTGCAC 1 cut(s) 2851
ApoI RAATTY 2 cut(s) 638, 2568
ArsI GACNNNNNNTTYG 2 cut(s) 1976, 2008
AseI ATTAAT 1 cut(s) 2783
Asp700I GAANNNNTTC 1 cut(s) 1299
AspA2I CCTAGG 1 cut(s) 50
AspLEI GCGC 4 cut(s) 333, 454, 1102, 1132
AsuC2I CCSGG 4 cut(s) 176, 177, 2698, 2699
AsuII TTCGAA 1 cut(s) 2418
AsuNHI GCTAGC 1 cut(s) 2177
AvaI CYCGRG 3 cut(s) 175, 1001, 2697
AvaII GGWCC 2 cut(s) 735, 1023
AvrII CCTAGG 1 cut(s) 50
AxyI CCTNAGG 1 cut(s) 539
BaeGI GKGCMC 1 cut(s) 2855
BalI TGGCCA 2 cut(s) 1154, 1526
BanII GRGCYC 3 cut(s) 310, 866, 1220
BarI GAAGNNNNNNTAC 2 cut(s) 924, 956
BauI CACGAG 2 cut(s) 200, 218
BbrPI CACGTG 1 cut(s) 1068
BbsI GAAGAC 4 cut(s) 749, 943, 1022, 2500
Bbv12I GWGCWC 4 cut(s) 866, 1220, 2620, 2855
BbvCI CCTCAGC 1 cut(s) 1950
BceAI ACGGC 5 cut(s) 3, 150, 547, 1113, 1601
BcgI CGANNNNNNTGC 2 cut(s) 813, 847
BclI TGATCA 1 cut(s) 2716
BcnI CCSGG 4 cut(s) 176, 177, 2698, 2699
BfaI CTAG 6 cut(s) 44, 51, 1041, 1310, 1721, 2178
BfmI CTRYAG 2 cut(s) 31, 1703
BfoI RGCGCY 2 cut(s) 334, 455
BfrI CTTAAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 2566
BglI GCCNNNNNGGC 2 cut(s) 463, 472
BglII AGATCT 3 cut(s) 147, 1197, 2032
BlnI CCTAGG 1 cut(s) 50
Bme1390I CCNGG 4 cut(s) 176, 177, 2698, 2699
Bme18I GGWCC 2 cut(s) 735, 1023
BmeT110I CYCGRG 3 cut(s) 175, 1001, 2697
BmiI GGNNCC 5 cut(s) 188, 258, 874, 1803, 2095
BmrFI CCNGG 4 cut(s) 176, 177, 2698, 2699
BmrI ACTGGG 1 cut(s) 904
BmsI GCATC 5 cut(s) 312, 1830, 2126, 2441, 2928
BmtI GCTAGC 1 cut(s) 2181
BmuI ACTGGG 1 cut(s) 904
BoxI GACNNNNGTC 1 cut(s) 974
BpiI GAAGAC 4 cut(s) 749, 943, 1022, 2500
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 1950
Bpu14I TTCGAA 1 cut(s) 2418
BpuEI CTTGAG 3 cut(s) 311, 2144, 2558
BpuMI CCSGG 4 cut(s) 176, 177, 2698, 2699
BsaAI YACGTR 1 cut(s) 1068
BsaI GGTCTC 2 cut(s) 414, 707
BsaJI CCNNGG 9 cut(s) 50, 174, 175, 467, 483, 1482, 2257, 2697, 2790
BsaWI WCCGGW 2 cut(s) 1403, 2391
BsaXI ACNNNNNCTCC 6 cut(s) 53, 83, 337, 367, 2107, 2137
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1744, 2391, 2951
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 6 cut(s) 612, 671, 805, 899, 2848, 3046
Bse21I CCTNAGG 1 cut(s) 539
Bse3DI GCAATG 2 cut(s) 1143, 2561
BseDI CCNNGG 9 cut(s) 50, 174, 175, 467, 483, 1482, 2257, 2697, 2790
BseGI GGATG 6 cut(s) 354, 918, 1322, 1375, 2236, 2800
BseLI CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1744, 2391, 2951
BseMI GCAATG 2 cut(s) 1143, 2561
BseMII CTCAG 3 cut(s) 852, 1941, 2475
BseNI ACTGG 6 cut(s) 612, 671, 805, 899, 2848, 3046
BseRI GAGGAG 6 cut(s) 358, 484, 695, 1508, 1967, 2747
BseSI GKGCMC 1 cut(s) 2855
BseX3I CGGCCG 1 cut(s) 532
BseYI CCCAGC 2 cut(s) 1469, 2684
BsgI GTGCAG 1 cut(s) 1610
Bsh1236I CGCG 2 cut(s) 132, 2757
Bsh1285I CGRYCG 1 cut(s) 535
BsiEI CGRYCG 1 cut(s) 535
BsiHKAI GWGCWC 4 cut(s) 866, 1220, 2620, 2855
BsiHKCI CYCGRG 3 cut(s) 175, 1001, 2697
BsiSI CCGG 7 cut(s) 176, 295, 699, 1112, 1404, 2392, 2698
BslFI GGGAC 2 cut(s) 1291, 1557
BslI CCNNNNNNNGG 7 cut(s) 182, 607, 985, 1032, 1744, 2391, 2951
BsmBI CGTCTC 3 cut(s) 384, 584, 957
BsmFI GGGAC 2 cut(s) 1291, 1557
BsmI GAATGC 2 cut(s) 1769, 2663
Bso31I GGTCTC 2 cut(s) 414, 707
BsoBI CYCGRG 3 cut(s) 175, 1001, 2697
Bsp119I TTCGAA 1 cut(s) 2418
Bsp1286I GDGCHC 5 cut(s) 310, 866, 1220, 2620, 2855
BspCNI CTCAG 3 cut(s) 851, 1942, 2474
BspFNI CGCG 2 cut(s) 132, 2757
BspHI TCATGA 3 cut(s) 495, 1258, 2719
BspLI GGNNCC 5 cut(s) 188, 258, 874, 1803, 2095
BspMAI CTGCAG 2 cut(s) 35, 1707
BspMI ACCTGC 1 cut(s) 2566
BspOI GCTAGC 1 cut(s) 2181
BspPI GGATC 3 cut(s) 1043, 2149, 2966
BspT104I TTCGAA 1 cut(s) 2418
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 2 cut(s) 414, 707
BsrDI GCAATG 2 cut(s) 1143, 2561
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 6 cut(s) 612, 671, 805, 899, 2848, 3046
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 9 cut(s) 50, 174, 175, 467, 483, 1482, 2257, 2697, 2790
BssSI CACGAG 2 cut(s) 200, 218
BssT1I CCWWGG 2 cut(s) 50, 2790
Bst2BI CACGAG 2 cut(s) 200, 218
Bst4CI ACNGT 6 cut(s) 119, 227, 1178, 1282, 1634, 1693
Bst6I CTCTTC 3 cut(s) 1859, 2063, 2595
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 2 cut(s) 2081, 2932
BstBAI YACGTR 1 cut(s) 1068
BstBI TTCGAA 1 cut(s) 2418
BstC8I GCNNGC 7 cut(s) 16, 101, 163, 1134, 1227, 2179, 2449
BstDEI CTNAG 4 cut(s) 539, 838, 1950, 2461
BstEII GGTNACC 2 cut(s) 277, 2231
BstENI CCTNNNNNAGG 1 cut(s) 983
BstF5I GGATG 6 cut(s) 354, 918, 1322, 1375, 2236, 2800
BstFNI CGCG 2 cut(s) 132, 2757
BstH2I RGCGCY 2 cut(s) 334, 455
BstHHI GCGC 4 cut(s) 333, 454, 1102, 1132
BstMCI CGRYCG 1 cut(s) 535
BstNSI RCATGY 2 cut(s) 2451, 2459
BstPAI GACNNNNGTC 1 cut(s) 974
BstPI GGTNACC 2 cut(s) 277, 2231
BstSCI CCNGG 4 cut(s) 174, 175, 2696, 2697
BstSFI CTRYAG 2 cut(s) 31, 1703
BstSLI GKGCMC 1 cut(s) 2855
BstUI CGCG 2 cut(s) 132, 2757
BstV2I GAAGAC 4 cut(s) 749, 943, 1022, 2500
BstX2I RGATCY 5 cut(s) 147, 1197, 2032, 2154, 2971
BstXI CCANNNNNNTGG 3 cut(s) 1333, 2216, 2797
BstYI RGATCY 5 cut(s) 147, 1197, 2032, 2154, 2971
BstZI CGGCCG 1 cut(s) 532
Bsu36I CCTNAGG 1 cut(s) 539
BtsCI GGATG 6 cut(s) 354, 918, 1322, 1375, 2236, 2800
BtsI GCAGTG 1 cut(s) 1506
BtsIMutI CAGTG 8 cut(s) 605, 664, 798, 892, 1506, 2607, 2853, 2855
BveI ACCTGC 1 cut(s) 2566
Cac8I GCNNGC 7 cut(s) 16, 101, 163, 1134, 1227, 2179, 2449
CaiI CAGNNNCTG 1 cut(s) 1505
CciI TCATGA 3 cut(s) 495, 1258, 2719
CfoI GCGC 4 cut(s) 333, 454, 1102, 1132
Cfr10I RCCGGY 1 cut(s) 698
Cfr9I CCCGGG 2 cut(s) 175, 2697
CseI GACGC 2 cut(s) 743, 1630
Csp6I GTAC 1 cut(s) 2068
CspCI CAANNNNNGTGG 2 cut(s) 255, 290
CviQI GTAC 1 cut(s) 2068
DdeI CTNAG 4 cut(s) 539, 838, 1950, 2461
EaeI YGGCCR 3 cut(s) 532, 1152, 1524
EagI CGGCCG 1 cut(s) 532
Eam1104I CTCTTC 3 cut(s) 1859, 2063, 2595
EarI CTCTTC 3 cut(s) 1859, 2063, 2595
EciI GGCGGA 6 cut(s) 503, 608, 874, 1160, 1250, 2828
Ecl136II GAGCTC 2 cut(s) 864, 1218
EclXI CGGCCG 1 cut(s) 532
Eco130I CCWWGG 2 cut(s) 50, 2790
Eco147I AGGCCT 1 cut(s) 1171
Eco24I GRGCYC 3 cut(s) 310, 866, 1220
Eco31I GGTCTC 2 cut(s) 414, 707
Eco32I GATATC 2 cut(s) 127, 958
Eco47I GGWCC 2 cut(s) 735, 1023
Eco47III AGCGCT 1 cut(s) 332
Eco52I CGGCCG 1 cut(s) 532
Eco53kI GAGCTC 2 cut(s) 864, 1218
Eco57I CTGAAG 2 cut(s) 1527, 1552
Eco72I CACGTG 1 cut(s) 1068
Eco81I CCTNAGG 1 cut(s) 539
Eco88I CYCGRG 3 cut(s) 175, 1001, 2697
Eco91I GGTNACC 2 cut(s) 277, 2231
EcoICRI GAGCTC 2 cut(s) 864, 1218
EcoNI CCTNNNNNAGG 1 cut(s) 983
EcoO65I GGTNACC 2 cut(s) 277, 2231
EcoRI GAATTC 2 cut(s) 638, 2568
EcoRV GATATC 2 cut(s) 127, 958
EcoT14I CCWWGG 2 cut(s) 50, 2790
EcoT22I ATGCAT 1 cut(s) 2434
EcoT38I GRGCYC 3 cut(s) 310, 866, 1220
ErhI CCWWGG 2 cut(s) 50, 2790
Esp3I CGTCTC 3 cut(s) 384, 584, 957
FalI AAGNNNNNCTT 6 cut(s) 397, 429, 1500, 1532, 1643, 1675
FaqI GGGAC 2 cut(s) 1291, 1557
FauI CCCGC 4 cut(s) 224, 247, 263, 1597
FauNDI CATATG 2 cut(s) 1807, 1936
FbaI TGATCA 1 cut(s) 2716
FblI GTMKAC 1 cut(s) 780
FokI GGATG 6 cut(s) 361, 905, 1309, 1382, 2223, 2807
FriOI GRGCYC 3 cut(s) 310, 866, 1220
FspBI CTAG 6 cut(s) 44, 51, 1041, 1310, 1721, 2178
GlaI GCGC 4 cut(s) 332, 453, 1101, 1131
GsaI CCCAGC 2 cut(s) 1473, 2688
GsuI CTGGAG 1 cut(s) 629
HaeII RGCGCY 2 cut(s) 334, 455
HapII CCGG 7 cut(s) 176, 295, 699, 1112, 1404, 2392, 2698
HgaI GACGC 2 cut(s) 743, 1630
HhaI GCGC 4 cut(s) 333, 454, 1102, 1132
Hin6I GCGC 4 cut(s) 331, 452, 1100, 1130
HinP1I GCGC 4 cut(s) 331, 452, 1100, 1130
HincII GTYRAC 2 cut(s) 781, 1885
HindII GTYRAC 2 cut(s) 781, 1885
HindIII AAGCTT 2 cut(s) 1446, 1509
HpaII CCGG 7 cut(s) 176, 295, 699, 1112, 1404, 2392, 2698
Hpy188III TCNNGA 8 cut(s) 435, 496, 761, 1001, 1259, 2030, 2324, 2720
Hpy99I CGWCG 6 cut(s) 343, 379, 533, 887, 986, 1099
HpyCH4III ACNGT 6 cut(s) 119, 227, 1178, 1282, 1634, 1693
HpyCH4IV ACGT 4 cut(s) 364, 885, 1067, 1388
HpyF3I CTNAG 4 cut(s) 539, 838, 1950, 2461
HpySE526I ACGT 4 cut(s) 364, 885, 1067, 1388
HspAI GCGC 4 cut(s) 331, 452, 1100, 1130
Ksp22I TGATCA 1 cut(s) 2716
LweI GCATC 5 cut(s) 312, 1830, 2126, 2441, 2928
MaeI CTAG 6 cut(s) 44, 51, 1041, 1310, 1721, 2178
MaeII ACGT 4 cut(s) 364, 885, 1067, 1388
MaeIII GTNAC 9 cut(s) 20, 119, 277, 514, 1477, 1501, 1618, 2231, 2884
MfeI CAATTG 2 cut(s) 1520, 2004
MflI RGATCY 5 cut(s) 147, 1197, 2032, 2154, 2971
MhlI GDGCHC 5 cut(s) 310, 866, 1220, 2620, 2855
MlsI TGGCCA 2 cut(s) 1154, 1526
MluI ACGCGT 1 cut(s) 2755
MluNI TGGCCA 2 cut(s) 1154, 1526
MlyI GAGTC 7 cut(s) 17, 62, 522, 641, 1748, 2141, 2489
MmeI TCCRAC 7 cut(s) 96, 237, 762, 891, 1264, 2523, 2969
Mox20I TGGCCA 2 cut(s) 1154, 1526
Mph1103I ATGCAT 1 cut(s) 2434
MroXI GAANNNNTTC 1 cut(s) 1299
MscI TGGCCA 2 cut(s) 1154, 1526
MseI TTAA 5 cut(s) 318, 1194, 2483, 2783, 3032
MslI CAYNNNNRTG 6 cut(s) 853, 927, 1484, 1826, 2214, 3006
Msp20I TGGCCA 2 cut(s) 1154, 1526
MspA1I CMGCKG 3 cut(s) 1601, 1949, 2822
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 7 cut(s) 176, 295, 699, 1112, 1404, 2392, 2698
MspR9I CCNGG 4 cut(s) 176, 177, 2698, 2699
MunI CAATTG 2 cut(s) 1520, 2004
Mva1269I GAATGC 2 cut(s) 1769, 2663
MvnI CGCG 2 cut(s) 132, 2757
NciI CCSGG 4 cut(s) 176, 177, 2698, 2699
NdeI CATATG 2 cut(s) 1807, 1936
NheI GCTAGC 1 cut(s) 2177
NlaIV GGNNCC 5 cut(s) 188, 258, 874, 1803, 2095
NmeAIII GCCGAG 3 cut(s) 464, 492, 2282
NmuCI GTSAC 9 cut(s) 20, 119, 277, 514, 1477, 1501, 1618, 2231, 2884
NsiI ATGCAT 1 cut(s) 2434
NspI RCATGY 2 cut(s) 2451, 2459
NspV TTCGAA 1 cut(s) 2418
OliI CACNNNNGTG 1 cut(s) 1484
PaeI GCATGC 1 cut(s) 2451
PaeR7I CTCGAG 1 cut(s) 1001
PagI TCATGA 3 cut(s) 495, 1258, 2719
PceI AGGCCT 1 cut(s) 1171
PciI ACATGT 1 cut(s) 2455
PcsI WCGNNNNNNNCGW 5 cut(s) 102, 129, 651, 990, 999
PctI GAATGC 2 cut(s) 1769, 2663
PdmI GAANNNNTTC 1 cut(s) 1299
PfeI GAWTC 4 cut(s) 1048, 1912, 2352, 2581
PflFI GACNNNGTC 2 cut(s) 83, 1021
PflMI CCANNNNNTGG 1 cut(s) 1744
PleI GAGTC 7 cut(s) 17, 62, 521, 641, 1748, 2141, 2489
PmaCI CACGTG 1 cut(s) 1068
PmlI CACGTG 1 cut(s) 1068
PpsI GAGTC 7 cut(s) 17, 62, 521, 641, 1748, 2141, 2489
Ppu21I YACGTR 1 cut(s) 1068
PscI ACATGT 1 cut(s) 2455
PshAI GACNNNNGTC 1 cut(s) 974
PshBI ATTAAT 1 cut(s) 2783
Psp124BI GAGCTC 2 cut(s) 866, 1220
PspCI CACGTG 1 cut(s) 1068
PspEI GGTNACC 2 cut(s) 277, 2231
PspFI CCCAGC 2 cut(s) 1469, 2684
PspN4I GGNNCC 5 cut(s) 188, 258, 874, 1803, 2095
PstI CTGCAG 2 cut(s) 35, 1707
PstNI CAGNNNCTG 1 cut(s) 1505
PsuI RGATCY 5 cut(s) 147, 1197, 2032, 2154, 2971
PsyI GACNNNGTC 2 cut(s) 83, 1021
PvuII CAGCTG 1 cut(s) 1949
RsaI GTAC 1 cut(s) 2069
RsaNI GTAC 1 cut(s) 2068
RseI CAYNNNNRTG 6 cut(s) 853, 927, 1484, 1826, 2214, 3006
SacI GAGCTC 2 cut(s) 866, 1220
SalI GTCGAC 1 cut(s) 779
SaqAI TTAA 5 cut(s) 318, 1194, 2483, 2783, 3032
SchI GAGTC 7 cut(s) 17, 62, 522, 641, 1748, 2141, 2489
ScrFI CCNGG 4 cut(s) 176, 177, 2698, 2699
SduI GDGCHC 5 cut(s) 310, 866, 1220, 2620, 2855
SfaNI GCATC 5 cut(s) 312, 1830, 2126, 2441, 2928
SfcI CTRYAG 2 cut(s) 31, 1703
SfiI GGCCNNNNNGGCC 1 cut(s) 472
Sfr274I CTCGAG 1 cut(s) 1001
SfuI TTCGAA 1 cut(s) 2418
SinI GGWCC 2 cut(s) 735, 1023
SlaI CTCGAG 1 cut(s) 1001
SmaI CCCGGG 2 cut(s) 177, 2699
SmiMI CAYNNNNRTG 6 cut(s) 853, 927, 1484, 1826, 2214, 3006
SmlI CTYRAG 5 cut(s) 317, 326, 1001, 2159, 2573
SmoI CTYRAG 5 cut(s) 317, 326, 1001, 2159, 2573
SphI GCATGC 1 cut(s) 2451
SseBI AGGCCT 1 cut(s) 1171
SspMI CTAG 6 cut(s) 44, 51, 1041, 1310, 1721, 2178
SstI GAGCTC 2 cut(s) 866, 1220
StuI AGGCCT 1 cut(s) 1171
StyD4I CCNGG 4 cut(s) 174, 175, 2696, 2697
StyI CCWWGG 2 cut(s) 50, 2790
TaaI ACNGT 6 cut(s) 119, 227, 1178, 1282, 1634, 1693
TaiI ACGT 4 cut(s) 367, 888, 1070, 1391
TatI WGTACW 1 cut(s) 2067
TauI GCSGC 6 cut(s) 457, 537, 679, 1130, 1604, 2379
TfiI GAWTC 4 cut(s) 1048, 1912, 2352, 2581
Tru1I TTAA 5 cut(s) 318, 1194, 2483, 2783, 3032
Tru9I TTAA 5 cut(s) 318, 1194, 2483, 2783, 3032
TscAI CASTG 8 cut(s) 612, 671, 805, 899, 1506, 2614, 2855, 2860
TseFI GTSAC 9 cut(s) 20, 119, 277, 514, 1477, 1501, 1618, 2231, 2884
Tsp45I GTSAC 9 cut(s) 20, 119, 277, 514, 1477, 1501, 1618, 2231, 2884
TspDTI ATGAA 8 cut(s) 1025, 1454, 1824, 1877, 1976, 2241, 2306, 2529
TspGWI ACGGA 2 cut(s) 327, 885
TspMI CCCGGG 2 cut(s) 175, 2697
TspRI CASTG 8 cut(s) 612, 671, 805, 899, 1506, 2614, 2855, 2860
Tth111I GACNNNGTC 2 cut(s) 83, 1021
Van91I CCANNNNNTGG 1 cut(s) 1744
Vha464I CTTAAG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 2851
VpaK11BI GGWCC 2 cut(s) 735, 1023
VspI ATTAAT 1 cut(s) 2783
XagI CCTNNNNNAGG 1 cut(s) 983
XapI RAATTY 2 cut(s) 638, 2568
XceI RCATGY 2 cut(s) 2451, 2459
XcmI CCANNNNNNNNNTGG 2 cut(s) 899, 2797
XhoI CTCGAG 1 cut(s) 1001
XmaI CCCGGG 2 cut(s) 175, 2697
XmaJI CCTAGG 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 1 cut(s) 1299
XspI CTAG 6 cut(s) 44, 51, 1041, 1310, 1721, 2178
Zsp2I ATGCAT 1 cut(s) 2434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.