MD16G1224300.v1.1

Plant family of unknown function (DUF810)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014182
Physical Location & Seq
Reverse (-)
22560050 .. 22566514
6465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1224300.v1.1.491

Sequence Viewer

Length: 3084 bp
ATGGGCCCCCACCACCACTCTCGCCGCGAGTCCCTCTGCAGCTCCCTTCTCGCACCCCGTCCCGACTACCACGACTGTGACCGCGACCTCGTCTGGCCCTTTGGCAAGCTTGAGGGCATCGACCGCGATGACGTGCGTGAGACCGCGTACGAGATCTTCTTTACCGCGTGTCGTTCTTCCCCTGGATTTGGAGGACGGAACGCCCTCGTGTTCTACTCCAACCACCACGATAACAACAGCAGTAATAGTAATGGTGGGGAGGGGAGCGGGTCCGGATCGGGGTCGAAGGTAAATGGGGTGGTGACAACGCCGACGAGCAGGGTGAAGCGGGCGCTCGGGCTGAAGATGCTGAAGCGGTCTCCGTCGAGGAGGATGGCGTTGGGCGCCGGAAATGGTGGATGGTCAAGTCCCTCCTCGCCGAACGCATCAAATAGTGGTGGCTCCCCCGGAATGTCGTTCACCTTGCCGCCCTCAAGGCCGAGGCGGCCAATGACATCCGCCGAGATTATGAGGCAGCAGATGAGGGTCACGGAAGGCAGCGATAATCGGCTGCGGAAGACGCTCATGAGGACCCTAGTAGGCCAAATGGGTAGGCGAGCAGAGACCATAATTCTCCCACTAGAACTCCTTCGACACCTAAAGCCATCCGAATTCAACGACTCCCACGAGTACCATTACTGGCAAAAGCGGCAGCTCAAGATCCTTGAAGCCGGTCTTCTTCTCCACCCTTCAATCCCACTAGCCAAGTCCAACACATTCGCTTTGCGTCTCAGAGAGATCATCAGAGCGGGTGACACCAAAGCCATAGACACAGGAAAGAACTCAGACACCATGAGAACCCTTTGCAACTCTGTAGTCTCCTTATCGTGGCGTAGCTCCAACGGAACTCCAACAGATGTTTGTCACTGGGCTGATGGCTACCCCCTCAACATCCACCTCTATGTTGCTCTCCTGCAATCAGTCTTCGACATCAGAGACGAGACACTAGTATTAGATGAAGTCGATGAAATGCTCGAGCTGATGAAGAAGACATGGTCGACATTGGGGATCACTAGGCCAATACACAATGTGTGCTTTACATGGGTGTTGTTTCAGCAATACGTGCAAACCGCACAGATTGAGGCCGACCTTTTGTGTGCTGCCCATGCTATGCTTGCAGAAGTGGCAAACAATGCCAAGAGGCCGGATAGGGAGGTTATGTATGTCAAGATTTTGTCTTCGGTGTTGAGCTCAATGCAGGGGTGGGCGGAGAAGAAATTGCTTCGGTACCATGATTATTTCCAAAGAGGGACAGTTGGCCAAATTGAGAACCTTCTCCCTTTGGCCTTGTCATCCTCCAAGATCTTGGGTGAAGATGTTACAATCACAGAGAGGGGAAGAGGAGGGAAAGGTGAAATAAAGGTGGTGGACAACTCCGGTGACCGCGTGGACTACTACATTCGATCTTCAATGAAACAAGCTTTTGCAAAGATTATGGAAGCTGGGAATGTAACTGAAGTGGCAGAAGATGCCGTGACTGAAACCTTGCATCAGTTAGCTAAAGAGACAGAGGAGTTGGCCTTGAAAGAGAGGGAAAGCTTCAGTCCCATACTCAAGAGGTGGCACACAACTGCAGCTGGTGTCGCGGCCGTGACACTTCACAACTGTTACGGAGCTGTGTTGAAGCAATACCTAAATGGGGTCTCCACGCTTTCAGGTGATACAGTTGAGATATTGCAAAGGGCAGGAAAACTAGAAAAGGTTCTGCTCCAAATGGTGGTTGAGGACTCTGCTGAGTGCGAAGATGGCGGCAAAGCAATTGTTAGAGAGATGGTTCCGTACGAAATTGATTCCATCATAATGAACCTCTTGAAAAAATGGATTCATGAGAGATTAAAGAGAGGGAAAGAGTGTGTTCACCGAGCGAAAGAAAGCGAGACATGGAATCCAAAGTCCAAATCGGAACCTTATGCGCAATCAGCTGAGGAGCTAATGAAATTGGCCAAGGAAACCGTGGATGACTTCTTCCAAATTCCGATAGGAATTACTGAAGATTTAGTTCAAGATCTTGCTGATGGGTTGGAGCATGTCTTCAAGGAGTATACTTCATTTGTTGCGTCTTGTGGTTCGAAACAGAGTTATATCCCCACACTTCCTCCTCTAACAAGATGCAATCGAGACTCGAAGTTCCTCAAGCTGTGGAAAAAAGCTAGCCCTTGTAGCGTTGGAGCAGAAGACTTTCACCCAAACGGAACAAATGAAGGTCACCATCCTCGCCCATCAACGAGTAGAGGAACACAACGCCTCTACATCCGCCTAAACACTTTGCACCATCTCCTTTCCCACCTTCATTCTCTTGACAAAAACCTCTCCCTCTCACCTCGCATCATTCCTACAACACCTCGAAGTCGTCATGCCAATAACCGCAAGAGTCAAACCAACGCCTCTTCCTACTTCGAACTTGCACAATCAGGCATCCAATCTGCCTGCCAACATGTCTCAGAAGTAGCTGCCTACCGCTTGATCTTTCTCGACTCAAACTCAGTCTTCTATGAGAGCCTCTATGTTGGTGATGTAGCCAATGCAAGAATAAGACCTGCGTTACGAATTCTCAAGCAGAATCTCACTCTCTTGGGAGTAATTCTCACAGATAAAGCGCAGGCCTTGGCAATCAAGGAAGTAATGAGGGCCTCTTTCGAAGCATTCCTCATGGTTTTGGTTGCTGGAGGAAGCTCCCGGGTGTTTTATCGAACTGACCATGAGATGATTGAGGAAGATTTCGACAGCTTAAAGCGTATTTTTTGCACTTGCGGAGAAGGGTTGATAACCAAGGATGTGGTGGAACATGAGGCAGAGACAACAGAAGGGGTGATAGAGCTGATGGGGCAATGTACTGAACAACTAATGGAGGATTTCAGCATTGTGACTTGCGAATCAAGCGGGATTGGAGTTGTGGGATCAGGGCAAAGGCTGCCAATGCCTCCGACAACAGGACGGTGGAACAGATCAGATCCAAACACATTATTGAGGGTGTTATGCCACAGGAATGACAAAGCAGCAAACCAATTCTTGAAAAGGACATTCCAGCTAGCGAAGAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF810

Protein Analysis

1028

Amino Acids

114.81

Weight (kDa)

8.51

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_PATROL1 PF25761 613 - 1027 3.2e-187 PATROL1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04470 AT2G33420
fragaria_vesca FvH4_4g04280 FvH4_4g04280
malus_domestica MD13G1219000.v1.1 MD16G1224300.v1.1
prunus_persica Prupe.1G045700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0394731
rosa_laevigata RLG00000009667
rosa_multiflora Rmu_sc0005356.1_g000011
rosa_roxburghii Rroxscaffold_5G00339750
rosa_rugosa Rorug03G0362100.1
rosa_samantha Rh4AG054800 Rh4BG052500 Rh4CG059300 Rh4DG051100
rosa_wichuraiana Rw4G004370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1953
Acc36I ACCTGC 1 cut(s) 2584
Acc65I GGTACC 1 cut(s) 1266
AccB1I GGYRCC 2 cut(s) 383, 1266
AccB7I CCANNNNNTGG 1 cut(s) 1756
AccBSI CCGCTC 2 cut(s) 267, 788
AccI GTMKAC 2 cut(s) 1037, 2081
AccII CGCG 7 cut(s) 27, 84, 126, 146, 167, 1425, 1625
AccIII TCCGGA 1 cut(s) 272
AclWI GGATC 5 cut(s) 283, 694, 1055, 2944, 2984
AcoI YGGCCR 4 cut(s) 485, 1297, 1626, 1980
AcsI RAATTY 3 cut(s) 650, 2010, 2586
AcuI CTGAAG 5 cut(s) 362, 371, 1515, 1564, 2049
AcyI GRCGYC 1 cut(s) 384
AdeI CACNNNGTG 1 cut(s) 1069
AfaI GTAC 5 cut(s) 149, 671, 1268, 1820, 2872
AfiI CCNNNNNNNGG 6 cut(s) 188, 279, 867, 1678, 1756, 2969
AflIII ACRYGT 1 cut(s) 2473
AhlI ACTAGT 1 cut(s) 985
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 1 cut(s) 181
AleI CACNNNNGTG 1 cut(s) 75
Alw21I GWGCWC 1 cut(s) 1232
AlwI GGATC 5 cut(s) 283, 694, 1055, 2944, 2984
Ama87I CYCGRG 3 cut(s) 335, 1013, 2715
Aor13HI TCCGGA 1 cut(s) 272
ApaI GGGCCC 1 cut(s) 8
ApoI RAATTY 3 cut(s) 650, 2010, 2586
Asp700I GAANNNNTTC 3 cut(s) 627, 1740, 2217
Asp718I GGTACC 1 cut(s) 1266
AspLEI GCGC 4 cut(s) 334, 386, 1954, 2638
AspS9I GGNCC 6 cut(s) 4, 5, 96, 270, 570, 2667
AsuC2I CCSGG 3 cut(s) 447, 2716, 2717
AsuII TTCGAA 3 cut(s) 2108, 2436, 2676
AsuNHI GCTAGC 2 cut(s) 2189, 3067
AvaI CYCGRG 3 cut(s) 335, 1013, 2715
AvaII GGWCC 2 cut(s) 270, 570
BaeGI GKGCMC 1 cut(s) 8
BalI TGGCCA 2 cut(s) 1299, 1982
BanI GGYRCC 2 cut(s) 383, 1266
BanII GRGCYC 2 cut(s) 8, 1232
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BauI CACGAG 2 cut(s) 206, 665
BbsI GAAGAC 8 cut(s) 563, 707, 955, 1034, 1209, 2062, 2220, 2518
Bbv12I GWGCWC 1 cut(s) 1232
BbvCI CCTCAGC 1 cut(s) 1962
BceAI ACGGC 2 cut(s) 1496, 1613
BciT130I CCWGG 1 cut(s) 183
BcnI CCSGG 3 cut(s) 447, 2716, 2717
BcuI ACTAGT 1 cut(s) 985
BfaI CTAG 8 cut(s) 575, 620, 740, 986, 1053, 1733, 2190, 3068
BfmI CTRYAG 3 cut(s) 37, 852, 1611
BfoI RGCGCY 2 cut(s) 335, 387
BfuAI ACCTGC 1 cut(s) 2584
BglI GCCNNNNNGGC 2 cut(s) 475, 484
BglII AGATCT 3 cut(s) 153, 1341, 2044
Bme1390I CCNGG 4 cut(s) 183, 447, 2716, 2717
Bme18I GGWCC 2 cut(s) 270, 570
BmeT110I CYCGRG 3 cut(s) 335, 1013, 2715
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 6 cut(s) 4, 5, 96, 270, 570, 2667
BmiI GGNNCC 9 cut(s) 6, 7, 271, 385, 442, 572, 1268, 1815, 1944
BmrFI CCNGG 4 cut(s) 183, 447, 2716, 2717
BmrI ACTGGG 1 cut(s) 916
BmsI GCATC 8 cut(s) 126, 336, 434, 1498, 1537, 2138, 2373, 2463
BmtI GCTAGC 2 cut(s) 2193, 3071
BmuI ACTGGG 1 cut(s) 916
BpiI GAAGAC 8 cut(s) 563, 707, 955, 1034, 1209, 2062, 2220, 2518
BplI GAGNNNNNCTC 2 cut(s) 2607, 2639
BpmI CTGGAG 1 cut(s) 2724
Bpu10I CCTNAGC 1 cut(s) 1962
Bpu14I TTCGAA 3 cut(s) 2108, 2436, 2676
BpuEI CTTGAG 6 cut(s) 131, 457, 680, 1577, 2156, 2576
BpuMI CCSGG 3 cut(s) 447, 2716, 2717
BsaAI YACGTR 1 cut(s) 1102
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 4 cut(s) 134, 363, 596, 1687
BsaJI CCNNGG 8 cut(s) 181, 445, 479, 1983, 1992, 2643, 2715, 2808
BsaWI WCCGGW 2 cut(s) 272, 1415
BsaXI ACNNNNNCTCC 6 cut(s) 609, 639, 1184, 1214, 2119, 2149
Bsc4I CCNNNNNNNGG 6 cut(s) 188, 279, 867, 1678, 1756, 2969
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 2 cut(s) 683, 911
Bse3DI GCAATG 1 cut(s) 2873
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 8 cut(s) 181, 445, 479, 1983, 1992, 2643, 2715, 2808
BseLI CCNNNNNNNGG 6 cut(s) 188, 279, 867, 1678, 1756, 2969
BseMI GCAATG 1 cut(s) 2873
BseMII CTCAG 6 cut(s) 784, 837, 1764, 1953, 2493, 2535
BseNI ACTGG 2 cut(s) 683, 911
BseRI GAGGAG 6 cut(s) 382, 403, 1395, 1565, 1979, 2127
BseSI GKGCMC 1 cut(s) 8
BseX3I CGGCCG 1 cut(s) 1626
BseYI CCCAGC 1 cut(s) 1481
Bsh1236I CGCG 7 cut(s) 27, 84, 126, 146, 167, 1425, 1625
Bsh1285I CGRYCG 2 cut(s) 124, 1629
BshNI GGYRCC 2 cut(s) 383, 1266
BsiEI CGRYCG 2 cut(s) 124, 1629
BsiHKAI GWGCWC 1 cut(s) 1232
BsiHKCI CYCGRG 3 cut(s) 335, 1013, 2715
BsiSI CCGG 7 cut(s) 273, 387, 447, 711, 1184, 1416, 2716
BsiWI CGTACG 2 cut(s) 147, 1818
BslFI GGGAC 5 cut(s) 16, 45, 393, 1303, 1569
BslI CCNNNNNNNGG 6 cut(s) 188, 279, 867, 1678, 1756, 2969
BsmBI CGTCTC 2 cut(s) 773, 969
BsmFI GGGAC 5 cut(s) 16, 45, 393, 1303, 1569
BsmI GAATGC 1 cut(s) 2681
Bso31I GGTCTC 4 cut(s) 134, 363, 596, 1687
BsoBI CYCGRG 3 cut(s) 335, 1013, 2715
Bsp119I TTCGAA 3 cut(s) 2108, 2436, 2676
Bsp120I GGGCCC 1 cut(s) 4
Bsp1286I GDGCHC 2 cut(s) 8, 1232
Bsp13I TCCGGA 1 cut(s) 272
BspCNI CTCAG 6 cut(s) 783, 836, 1765, 1954, 2492, 2534
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 7 cut(s) 27, 84, 126, 146, 167, 1425, 1625
BspHI TCATGA 2 cut(s) 564, 1864
BspLI GGNNCC 9 cut(s) 6, 7, 271, 385, 442, 572, 1268, 1815, 1944
BspMAI CTGCAG 2 cut(s) 41, 1615
BspMI ACCTGC 1 cut(s) 2584
BspOI GCTAGC 2 cut(s) 2193, 3071
BspPI GGATC 5 cut(s) 283, 694, 1055, 2944, 2984
BspT104I TTCGAA 3 cut(s) 2108, 2436, 2676
BspT107I GGYRCC 2 cut(s) 383, 1266
BspTNI GGTCTC 4 cut(s) 134, 363, 596, 1687
BsrBI CCGCTC 2 cut(s) 267, 788
BsrDI GCAATG 1 cut(s) 2873
BsrFI RCCGGY 1 cut(s) 710
BsrI ACTGG 2 cut(s) 683, 911
BssAI RCCGGY 1 cut(s) 710
BssECI CCNNGG 8 cut(s) 181, 445, 479, 1983, 1992, 2643, 2715, 2808
BssNAI GTATAC 1 cut(s) 2082
BssNI GRCGYC 1 cut(s) 384
BssSI CACGAG 2 cut(s) 206, 665
BssT1I CCWWGG 3 cut(s) 1983, 2643, 2808
Bst1107I GTATAC 1 cut(s) 2082
Bst2BI CACGAG 2 cut(s) 206, 665
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 6 cut(s) 77, 1294, 1646, 1705, 1993, 2976
Bst6I CTCTTC 3 cut(s) 1372, 2431, 3068
BstACI GRCGYC 1 cut(s) 384
BstAPI GCANNNNNTGC 4 cut(s) 1102, 1172, 1508, 2950
BstBAI YACGTR 1 cut(s) 1102
BstBI TTCGAA 3 cut(s) 2108, 2436, 2676
BstC8I GCNNGC 8 cut(s) 107, 330, 597, 1155, 2191, 2467, 2640, 3069
BstDEI CTNAG 6 cut(s) 770, 823, 1773, 1962, 2479, 2521
BstDSI CCRYGG 1 cut(s) 1992
BstEII GGTNACC 2 cut(s) 1418, 2243
BstFNI CGCG 7 cut(s) 27, 84, 126, 146, 167, 1425, 1625
BstH2I RGCGCY 2 cut(s) 335, 387
BstHHI GCGC 4 cut(s) 334, 386, 1954, 2638
BstMCI CGRYCG 2 cut(s) 124, 1629
BstNI CCWGG 1 cut(s) 183
BstNSI RCATGY 2 cut(s) 2069, 2477
BstPI GGTNACC 2 cut(s) 1418, 2243
BstSCI CCNGG 4 cut(s) 181, 445, 2714, 2715
BstSFI CTRYAG 3 cut(s) 37, 852, 1611
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 7 cut(s) 27, 84, 126, 146, 167, 1425, 1625
BstV2I GAAGAC 8 cut(s) 563, 707, 955, 1034, 1209, 2062, 2220, 2518
BstX2I RGATCY 5 cut(s) 153, 699, 1341, 2044, 2989
BstXI CCANNNNNNTGG 2 cut(s) 1345, 2815
BstYI RGATCY 5 cut(s) 153, 699, 1341, 2044, 2989
BstZ17I GTATAC 1 cut(s) 2082
BstZI CGGCCG 1 cut(s) 1626
BtgI CCRYGG 1 cut(s) 1992
BtgZI GCGATG 1 cut(s) 141
BtrI CACGTC 1 cut(s) 133
BtsIMutI CAGTG 1 cut(s) 904
BveI ACCTGC 1 cut(s) 2584
Cac8I GCNNGC 8 cut(s) 107, 330, 597, 1155, 2191, 2467, 2640, 3069
CciI TCATGA 2 cut(s) 564, 1864
CfoI GCGC 4 cut(s) 334, 386, 1954, 2638
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 6 cut(s) 4, 5, 96, 270, 570, 2667
Cfr9I CCCGGG 1 cut(s) 2715
CseI GACGC 3 cut(s) 568, 755, 2085
Csp6I GTAC 5 cut(s) 148, 670, 1267, 1819, 2871
CviQI GTAC 5 cut(s) 148, 670, 1267, 1819, 2871
DdeI CTNAG 6 cut(s) 770, 823, 1773, 1962, 2479, 2521
DinI GGCGCC 1 cut(s) 385
DraIII CACNNNGTG 1 cut(s) 1069
EaeI YGGCCR 4 cut(s) 485, 1297, 1626, 1980
EagI CGGCCG 1 cut(s) 1626
Eam1104I CTCTTC 3 cut(s) 1372, 2431, 3068
EarI CTCTTC 3 cut(s) 1372, 2431, 3068
EciI GGCGGA 3 cut(s) 487, 1262, 2282
Ecl136II GAGCTC 1 cut(s) 1230
EclXI CGGCCG 1 cut(s) 1626
Eco130I CCWWGG 3 cut(s) 1983, 2643, 2808
Eco147I AGGCCT 1 cut(s) 2642
Eco24I GRGCYC 2 cut(s) 8, 1232
Eco31I GGTCTC 4 cut(s) 134, 363, 596, 1687
Eco47I GGWCC 2 cut(s) 270, 570
Eco52I CGGCCG 1 cut(s) 1626
Eco53kI GAGCTC 1 cut(s) 1230
Eco57I CTGAAG 5 cut(s) 362, 371, 1515, 1564, 2049
Eco88I CYCGRG 3 cut(s) 335, 1013, 2715
Eco91I GGTNACC 2 cut(s) 1418, 2243
EcoICRI GAGCTC 1 cut(s) 1230
EcoO109I RGGNCCY 3 cut(s) 5, 570, 2667
EcoO65I GGTNACC 2 cut(s) 1418, 2243
EcoRI GAATTC 2 cut(s) 650, 2586
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 3 cut(s) 1983, 2643, 2808
EcoT38I GRGCYC 2 cut(s) 8, 1232
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
ErhI CCWWGG 3 cut(s) 1983, 2643, 2808
Esp3I CGTCTC 2 cut(s) 773, 969
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 5 cut(s) 16, 45, 393, 1303, 1569
FauI CCCGC 4 cut(s) 260, 321, 781, 2912
FblI GTMKAC 2 cut(s) 1037, 2081
FriOI GRGCYC 2 cut(s) 8, 1232
FspBI CTAG 8 cut(s) 575, 620, 740, 986, 1053, 1733, 2190, 3068
FspI TGCGCA 1 cut(s) 1953
GlaI GCGC 4 cut(s) 333, 385, 1953, 2637
GsaI CCCAGC 1 cut(s) 1485
GsuI CTGGAG 1 cut(s) 2724
HaeII RGCGCY 2 cut(s) 335, 387
HapII CCGG 7 cut(s) 273, 387, 447, 711, 1184, 1416, 2716
HgaI GACGC 3 cut(s) 568, 755, 2085
HhaI GCGC 4 cut(s) 334, 386, 1954, 2638
Hin1I GRCGYC 1 cut(s) 384
Hin6I GCGC 4 cut(s) 332, 384, 1952, 2636
HinP1I GCGC 4 cut(s) 332, 384, 1952, 2636
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HindIII AAGCTT 3 cut(s) 107, 1458, 1576
HpaII CCGG 7 cut(s) 273, 387, 447, 711, 1184, 1416, 2716
Hpy166II GTNNAC 6 cut(s) 459, 1038, 1408, 1429, 1897, 2082
Hpy8I GTNNAC 6 cut(s) 459, 1038, 1408, 1429, 1897, 2082
Hpy99I CGWCG 2 cut(s) 316, 367
HpyCH4III ACNGT 6 cut(s) 77, 1294, 1646, 1705, 1993, 2976
HpyCH4IV ACGT 2 cut(s) 132, 1101
HpyF3I CTNAG 6 cut(s) 770, 823, 1773, 1962, 2479, 2521
HpySE526I ACGT 2 cut(s) 132, 1101
Hsp92I GRCGYC 1 cut(s) 384
HspAI GCGC 4 cut(s) 332, 384, 1952, 2636
KasI GGCGCC 1 cut(s) 383
Kpn2I TCCGGA 1 cut(s) 272
KpnI GGTACC 1 cut(s) 1270
LweI GCATC 8 cut(s) 126, 336, 434, 1498, 1537, 2138, 2373, 2463
MaeI CTAG 8 cut(s) 575, 620, 740, 986, 1053, 1733, 2190, 3068
MaeII ACGT 2 cut(s) 132, 1101
MbiI CCGCTC 2 cut(s) 267, 788
MfeI CAATTG 1 cut(s) 1797
MflI RGATCY 5 cut(s) 153, 699, 1341, 2044, 2989
MhlI GDGCHC 2 cut(s) 8, 1232
MlsI TGGCCA 2 cut(s) 1299, 1982
MluNI TGGCCA 2 cut(s) 1299, 1982
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 6 cut(s) 38, 653, 1760, 2153, 2419, 2507
MmeI TCCRAC 7 cut(s) 243, 774, 903, 914, 2040, 2185, 2987
Mox20I TGGCCA 2 cut(s) 1299, 1982
MroI TCCGGA 1 cut(s) 272
MroXI GAANNNNTTC 3 cut(s) 627, 1740, 2217
MscI TGGCCA 2 cut(s) 1299, 1982
MseI TTAA 2 cut(s) 1874, 2768
MslI CAYNNNNRTG 4 cut(s) 75, 939, 1838, 3024
Msp20I TGGCCA 2 cut(s) 1299, 1982
MspA1I CMGCKG 2 cut(s) 1616, 1961
MspI CCGG 7 cut(s) 273, 387, 447, 711, 1184, 1416, 2716
MspR9I CCNGG 4 cut(s) 183, 447, 2716, 2717
MunI CAATTG 1 cut(s) 1797
Mva1269I GAATGC 1 cut(s) 2681
MvaI CCWGG 1 cut(s) 183
MvnI CGCG 7 cut(s) 27, 84, 126, 146, 167, 1425, 1625
NarI GGCGCC 1 cut(s) 384
NciI CCSGG 3 cut(s) 447, 2716, 2717
NheI GCTAGC 2 cut(s) 2189, 3067
NlaIV GGNNCC 9 cut(s) 6, 7, 271, 385, 442, 572, 1268, 1815, 1944
NmeAIII GCCGAG 2 cut(s) 504, 526
NsbI TGCGCA 1 cut(s) 1953
NspI RCATGY 2 cut(s) 2069, 2477
NspV TTCGAA 3 cut(s) 2108, 2436, 2676
OliI CACNNNNGTG 1 cut(s) 75
PaeR7I CTCGAG 1 cut(s) 1013
PagI TCATGA 2 cut(s) 564, 1864
PceI AGGCCT 1 cut(s) 2642
PciI ACATGT 1 cut(s) 2473
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PctI GAATGC 1 cut(s) 2681
PdmI GAANNNNTTC 3 cut(s) 627, 1740, 2217
PfeI GAWTC 5 cut(s) 1829, 1861, 1924, 2599, 2912
Pfl23II CGTACG 2 cut(s) 147, 1818
PflFI GACNNNGTC 2 cut(s) 89, 1033
PflMI CCANNNNNTGG 1 cut(s) 1756
PleI GAGTC 6 cut(s) 37, 653, 1760, 2153, 2418, 2507
PluTI GGCGCC 1 cut(s) 387
PpsI GAGTC 6 cut(s) 37, 653, 1760, 2153, 2418, 2507
Ppu21I YACGTR 1 cut(s) 1102
PpuMI RGGWCCY 1 cut(s) 570
PscI ACATGT 1 cut(s) 2473
Psp124BI GAGCTC 1 cut(s) 1232
Psp5II RGGWCCY 1 cut(s) 570
Psp6I CCWGG 1 cut(s) 181
PspEI GGTNACC 2 cut(s) 1418, 2243
PspFI CCCAGC 1 cut(s) 1481
PspGI CCWGG 1 cut(s) 181
PspLI CGTACG 2 cut(s) 147, 1818
PspN4I GGNNCC 9 cut(s) 6, 7, 271, 385, 442, 572, 1268, 1815, 1944
PspOMI GGGCCC 1 cut(s) 4
PspPI GGNCC 6 cut(s) 4, 5, 96, 270, 570, 2667
PspPPI RGGWCCY 1 cut(s) 570
PspXI VCTCGAGB 1 cut(s) 1013
PstI CTGCAG 2 cut(s) 41, 1615
PsuI RGATCY 5 cut(s) 153, 699, 1341, 2044, 2989
PsyI GACNNNGTC 2 cut(s) 89, 1033
PvuII CAGCTG 2 cut(s) 1616, 1961
RsaI GTAC 5 cut(s) 149, 671, 1268, 1820, 2872
RsaNI GTAC 5 cut(s) 148, 670, 1267, 1819, 2871
RseI CAYNNNNRTG 4 cut(s) 75, 939, 1838, 3024
SacI GAGCTC 1 cut(s) 1232
SalI GTCGAC 1 cut(s) 1036
SaqAI TTAA 2 cut(s) 1874, 2768
Sau96I GGNCC 6 cut(s) 4, 5, 96, 270, 570, 2667
SchI GAGTC 6 cut(s) 38, 653, 1760, 2153, 2419, 2507
ScrFI CCNGG 4 cut(s) 183, 447, 2716, 2717
SduI GDGCHC 2 cut(s) 8, 1232
SfaNI GCATC 8 cut(s) 126, 336, 434, 1498, 1537, 2138, 2373, 2463
SfcI CTRYAG 3 cut(s) 37, 852, 1611
SfiI GGCCNNNNNGGCC 1 cut(s) 484
SfoI GGCGCC 1 cut(s) 385
Sfr274I CTCGAG 1 cut(s) 1013
SfuI TTCGAA 3 cut(s) 2108, 2436, 2676
SinI GGWCC 2 cut(s) 270, 570
SlaI CTCGAG 1 cut(s) 1013
SmaI CCCGGG 1 cut(s) 2717
SmiMI CAYNNNNRTG 4 cut(s) 75, 939, 1838, 3024
SmlI CTYRAG 7 cut(s) 110, 472, 695, 1013, 1592, 2171, 2591
SmoI CTYRAG 7 cut(s) 110, 472, 695, 1013, 1592, 2171, 2591
SpeI ACTAGT 1 cut(s) 985
SseBI AGGCCT 1 cut(s) 2642
SspDI GGCGCC 1 cut(s) 383
SspMI CTAG 8 cut(s) 575, 620, 740, 986, 1053, 1733, 2190, 3068
SstI GAGCTC 1 cut(s) 1232
StuI AGGCCT 1 cut(s) 2642
StyD4I CCNGG 4 cut(s) 181, 445, 2714, 2715
StyI CCWWGG 3 cut(s) 1983, 2643, 2808
TaaI ACNGT 6 cut(s) 77, 1294, 1646, 1705, 1993, 2976
TaiI ACGT 2 cut(s) 135, 1104
TatI WGTACW 1 cut(s) 2870
TauI GCSGC 6 cut(s) 27, 469, 487, 691, 1628, 1791
TfiI GAWTC 5 cut(s) 1829, 1861, 1924, 2599, 2912
Tru1I TTAA 2 cut(s) 1874, 2768
Tru9I TTAA 2 cut(s) 1874, 2768
TscAI CASTG 1 cut(s) 911
TspGWI ACGGA 7 cut(s) 211, 351, 545, 897, 1665, 1806, 2244
TspMI CCCGGG 1 cut(s) 2715
TspRI CASTG 1 cut(s) 911
Tth111I GACNNNGTC 2 cut(s) 89, 1033
Van91I CCANNNNNTGG 1 cut(s) 1756
VpaK11BI GGWCC 2 cut(s) 270, 570
XapI RAATTY 3 cut(s) 650, 2010, 2586
XceI RCATGY 2 cut(s) 2069, 2477
XcmI CCANNNNNNNNNTGG 2 cut(s) 1990, 2815
XhoI CTCGAG 1 cut(s) 1013
XmaI CCCGGG 1 cut(s) 2715
XmiI GTMKAC 2 cut(s) 1037, 2081
XmnI GAANNNNTTC 3 cut(s) 627, 1740, 2217
XspI CTAG 8 cut(s) 575, 620, 740, 986, 1053, 1733, 2190, 3068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.