Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0009572

Physical Location & Seq

Genomic Coordinates Forward (+)

21737508 .. 21750111

Gene Structure 12604 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1223200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3423 bp

ATGTACTGGCTCGCTACTCGGAACGGCGTCGCCTCTTTCCCTCGCTGCTGCCATCTCGCTCTTCTTCTTCGCTCCCCTCCTCGTAAATGCAGCTCTTTCATTCCCTCTCCTCCGCTCATTGGACAGTTTCGAAGGATTCGTTGTTTCAAAGATCAAAAAGTTTCAGGAGGAAGCAGAAAAGGGACCAAAAAACTTAATGCTTTGAATAATTTCCTGGATGAAAGAGCTCTTTCGAACATACTGTGGTGGAAGGAGAGGATGGAGATGTGCAGGAAGCCTTCAACTGTCCAGCTGGTTAAAAGGCTTGACTATTCCAATTTGCTAGGCTTGGACGTTAACTTAAAAAATGGGAGTCTAAAAGAAGGAACACTCAACTGGGAGATCTTGCAGTTCAAGTCAAAGTTTCCACATGAAGTTTTGCTCTGCAGAGTTGGTGACTTTTATGAAGCCCTTGGAATAGATGCTTGTATTTTGGTTGAATATGCAGGTTTAAATCCTTTTGGGGGTCTGCGCTCAGATAGTATTCCAAGAGCTGGTTGCCCGGTTGTGAATCTTCGTCAAACTTTGGATGATCTGACACGGAATGGGTTTTCGGTGTGCATTGTGGAAGAAGTGCAGGGCCCAACACAAGCTCGTTCCCGTAAAGGGCGTTTTATATCGGGGCATGCTCATCCTGGTAGTCCTTACGTCTTCGGACTTGTTGGGGTTGATCACGATCTTGATTTTCCAGAACCAATGCCTGTTGTTGGAATATCTCGTTCCGCAAGAGGGTATTGCATCAATTTTGTCCTGGAGACTATGAAAACTTATTCGTCGGAGGATGGTTTGACTGAGGAGGCCTTGGTTACTAAGCTTCGTACATGTCGGTACCATCATTTATTTCTGCATACGTCTTTGAGGAGCAATTTCTCAGGCACTTGTCGTTGGGGAGAATTTGGTGAGGGAGGCTTACTGTGGGGAGAATGTAATGGTAGACATTTTGAATGGTTTGAAGGCAATCCTGTCACTGAGATTTTATCTAAGGTTAAAGATCTTTATGGTCTAGATGAAGAAGTTACATTCAGGAATGTGAGTGTGCCTTCAGAAAACAGACCCCACCCTTTAACCCTTGGAACAGCGACTCAAATTGGTGCCATACCGACTGAGGGCATACCTTGTTTGTTGAAGGTGTTGCTTCCTTCAAATTGCACAGGCCTCCCCTTATTGTATGTTAGGGATCTTCTTCTTAATCCTCCAGCTTATGATATATCATCCACAATTCAAGCAACGTGCAGACTTATGAGCAACATAACGTGCTCAATTCCTGAGTTTACTTGTGTTTCACCTGCAAAGCTGGTGAAGCTTCTTGAACTGAGGGAGGCCAATCATATTGAGTTCTGCAGAATTAAAAATGTTCTCGATGAAATATTGCACATGCAAAAAGCCCCTGAGCTATGTGAAATCCTGCAGTTGTTGATGGATCCTACATGGGTGGCAACTGGGTTGAAAATTGACTTTGAGACATTAGTTAATGAATGCGAGTGCACTTCAGATAGTATTGGTGAAATGATCTCCCTGGATGGAGAACATGACCAAAAGTTTAGTTCTTTTCTTCTTGTTCCTAATGATTTTTTTGAGGATATGGAGTCTTCATGGAAAGGTCGTATAAAGAGAATGCATATCGAGGAAGCAGTTGCAGAAGTGGAAAAGGCTGCTGAGGCGTTATCTCTAGCAGTTACTGAAGATTTCCTGCCTATTATTTCTAGAATAAAGGCCACCACAGCTCCACTTGGAGGCCCAAAAGGAGAAATATTATATGCACGGGAGCACGAAGCTGTTTGGTTTAAGGGTAAACGGTTTGCACCTGCTGTTTGGGGTGGTACCCCTGGGGAAGAACAGATTAAACAGCTTAAACCTGCGCTAGATTCAAAAGGTAGAAAGGTTGGAGAAGAGTGGTTTACCACAGCGAAAGTGGAGGACGCTTTAACAAGGTACCATGAGGCAGGTGCCAAGGCCAAAACAAGGGTATTGGAATTGTTGAGGGGACTTTCTTCTGATCTACAAGCGAAGATCAATATCCTTGTATTCTCCTCAATGCTTCTAGTTATTGCAAAGGCATTATTTGCTCATGTGAGTGAAGGGAGAAGAAGGAAATGGGTATTTCCCACCCTTGGAGAGTCCTGTAGGTCGAGGGATGTAAAACCATTGAATGGAGGAAATGGGATGAAGATAGTTGGTTTATCGCCATACTGGTTGAATGTAGCAGAGGGTAGTGCTGTAAATAACACTGTTGATATGCAGTCATTGTTTCTTTTGACTGGACCAAACGGTGGTGGTAAATCAAGTTTGTTACGATCAATTTGTGCTGCCGCATTACTTGGAATATGTGGATTTATGGTGCCTGCAGAATCAGCCTCGATTCCTCATTTTGATTCGATTATGCTTCACATGAAATCTTATGACAGCCCTGCTGATGGGAAAAGTTCCTTTCAGGTGGAGATGTCAGAAATTCGGTCCATTGTTACTGGTGCCACTAAGAGAAGTCTTGTACTTGTAGATGAAATCTGCCGAGGAACAGAAACAGCAAAGGGTACATGTATTGCTGGTAGTATTGTTGAAACTCTTGATGCAATAGGTTGTCTTGGTATCATATCTACTCACTTGCATGGGATATTTAGTTTGCCTCTTAATACGAAGAACACTGTAAACAAAGCAATGGGAACTGTATATGTGGATGGCCAAACAAAACCGACCTGGAAGTTGATGGATGGGATCTGTAGAGAAAGCTTAGCATTTGAAACGGCTAAGAGGGAAGGGATTCCTGAAACAATAATCGACAGAGCTGAAGACCTGTATCATTCAGTTTACGCAAATGAGGTACTTATAGGAAAGAATGACACAAAAGTTGAACAGTTAAGTTCCACAGGTTTTAGCAATTCTGAGAGATCCCATCCCCTGTCAAGTAGTGCTAAAGTTGAAGCTGTGACAGGATCAATGAACAGAATGGAAGTCCTACAGAAGGAGGTTGAGAGTGCAATGACCTTGATTTGCCAAAAGATGCTGACTGAGCTCTATAAGAAGAAAAAAACATCAGAACTTACTGAGATACGCTGTGTTCTAATTGGTACAAGGGAACAGCCACCTCCATCAACCATTGGTCTGTCATGTGTTTATGTGATTCTCAGGCCTGATAAGCGACTGTATGTCGGGCAGACGGATGATCTGGAGGGACGAGTCCGTGCACATCGTTCAAAGGAAGGACTGCAGAACGCCAATTTCCTTTATTTCACTGTCCCGGGGAAGAGCTTGGCTTGCCAATTAGAGACTCTTCTAATCAACCAGCTCCCTAATCAAGGATACCATCTGATCAATGTGGCTGATGGTAAGCATAGGAATTTCGGCACGTCCAATCTTTCCTTGGATGGTGTGGCAGTTTGTAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007005 GO:0008094 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009507 GO:0009536 GO:0009579 GO:0009628 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032042 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034357 GO:0034641 GO:0042623 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044436 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GIY-YIG MutS_I MutS_V

Protein Analysis

1141

Amino Acids

126.17

Weight (kDa)

7.8

Isoelectric Point (pI)

42.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	130 - 219	7.5e-10	MutS domain I
MutS_V	PF00488	764 - 946	9e-41	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24320 AT3G24320
fragaria_vesca	FvH4_4g05000 FvH4_4g05000 FvH4_4g05000
malus_domestica	MD13G1223200.v1.1
prunus_persica	Prupe.1G050200_v2.0.a1
pyrus_communis	pycom13g19710
rosa_chinensis	RchiOBHm_Chr4g0395811
rosa_laevigata	RLG00000009591
rosa_multiflora	Rmu_sc0001103.1_g000036 Rmu_sc0008803.1_g000001
rosa_roxburghii	Rroxscaffold_5G00340650
rosa_rugosa	Rorug03G0354100 Rorug03G0354200
rosa_samantha	Rh4AG062500 Rh4AG063500 Rh4AG067100 Rh4BG062200 Rh4CG068500 Rh4DG057100
rosa_wichuraiana	Rw4G005140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	1333, 1852, 1973
Acc36I	ACCTGC	5 cut(s)	476, 1333, 1852, 1903, 1973
Acc65I	GGTACC	3 cut(s)	867, 1859, 1971
AccB1I	GGYRCC	7 cut(s)	867, 1130, 1859, 1971, 1985, 2377, 2507
AccB7I	CCANNNNNTGG	3 cut(s)	533, 2189, 2309
AccBSI	CCGCTC	1 cut(s)	115
AccI	GTMKAC	1 cut(s)	973
AciI	CCGC	3 cut(s)	113, 762, 2349
AclWI	GGATC	6 cut(s)	1224, 1454, 1467, 2759, 2919, 2977
AcoI	YGGCCR	1 cut(s)	2716
AcsI	RAATTY	3 cut(s)	932, 2487, 3373
AcuI	CTGAAG	4 cut(s)	1065, 1512, 1740, 2844
AcyI	GRCGYC	1 cut(s)	27
AfaI	GTAC	9 cut(s)	5, 859, 869, 1861, 1973, 2529, 2572, 2859, 3106
AflIII	ACRYGT	2 cut(s)	860, 2573
AhdI	GACNNNNNGTC	1 cut(s)	3182
AjiI	CACGTC	1 cut(s)	3384
AjnI	CCWGG	6 cut(s)	213, 673, 789, 1554, 1864, 2732
Alw21I	GWGCWC	6 cut(s)	229, 1298, 1526, 1809, 3051, 3223
Alw26I	GTCTC	3 cut(s)	788, 1493, 3296
Alw44I	GTGCAC	2 cut(s)	1522, 3219
AlwI	GGATC	6 cut(s)	1224, 1454, 1467, 2759, 2919, 2977
AlwNI	CAGNNNCTG	1 cut(s)	1718
Ama87I	CYCGRG	1 cut(s)	3274
AoxI	GGCC	9 cut(s)	619, 837, 1192, 1359, 1752, 1774, 1992, 2716, 3164
ApaI	GGGCCC	1 cut(s)	623
ApaLI	GTGCAC	2 cut(s)	1522, 3219
ApeKI	GCWGC	5 cut(s)	45, 48, 90, 1691, 2345
ApoI	RAATTY	3 cut(s)	932, 2487, 3373
Asp700I	GAANNNNTTC	2 cut(s)	209, 2796
Asp718I	GGTACC	3 cut(s)	867, 1859, 1971
AspLEI	GCGC	2 cut(s)	513, 1900
AspS9I	GGNCC	6 cut(s)	183, 619, 620, 1775, 2300, 2493
AsuC2I	CCSGG	3 cut(s)	542, 3275, 3276
AsuHPI	GGTGA	5 cut(s)	446, 950, 1314, 1348, 1553
AsuII	TTCGAA	2 cut(s)	130, 233
AvaI	CYCGRG	1 cut(s)	3274
AvaII	GGWCC	3 cut(s)	183, 2300, 2493
BaeGI	GKGCMC	3 cut(s)	623, 1526, 3223
BalI	TGGCCA	1 cut(s)	2718
BamHI	GGATCC	1 cut(s)	1459
BanI	GGYRCC	7 cut(s)	867, 1130, 1859, 1971, 1985, 2377, 2507
BanII	GRGCYC	3 cut(s)	229, 623, 3051
BbsI	GAAGAC	3 cut(s)	682, 1620, 2832
Bbv12I	GWGCWC	6 cut(s)	229, 1298, 1526, 1809, 3051, 3223
BbvCI	CCTCAGC	1 cut(s)	1695
BbvI	GCAGC	5 cut(s)	32, 35, 102, 1678, 2332
BceAI	ACGGC	2 cut(s)	40, 2796
BciT130I	CCWGG	6 cut(s)	215, 675, 791, 1556, 1866, 2734
BciVI	GTATCC	1 cut(s)	3329
BclI	TGATCA	2 cut(s)	709, 3345
BcnI	CCSGG	3 cut(s)	542, 3275, 3276
BcoDI	GTCTC	3 cut(s)	788, 1493, 3296
BfaI	CTAG	6 cut(s)	323, 1043, 1709, 1743, 1901, 2081
BfmI	CTRYAG	8 cut(s)	424, 1378, 1445, 2161, 2382, 2755, 2993, 3242
BfuAI	ACCTGC	5 cut(s)	476, 1333, 1852, 1903, 1973
BfuI	GTATCC	1 cut(s)	3329
BglII	AGATCT	2 cut(s)	381, 1030
BisI	GCNGC	6 cut(s)	46, 49, 91, 1692, 2346, 2349
BlpI	GCTNAGC	1 cut(s)	2767
BlsI	GCNGC	6 cut(s)	47, 50, 92, 1693, 2347, 2350
Bme1390I	CCNGG	9 cut(s)	215, 542, 675, 791, 1556, 1866, 2734, 3275, 3276
Bme18I	GGWCC	3 cut(s)	183, 2300, 2493
BmeRI	GACNNNNNGTC	1 cut(s)	3182
BmeT110I	CYCGRG	1 cut(s)	3274
BmgBI	CACGTC	1 cut(s)	3384
BmgT120I	GGNCC	6 cut(s)	183, 619, 620, 1775, 2300, 2493
BmrFI	CCNGG	9 cut(s)	215, 542, 675, 791, 1556, 1866, 2734, 3275, 3276
BmrI	ACTGGG	2 cut(s)	385, 1488
BmsI	GCATC	4 cut(s)	451, 786, 2596, 3027
BmuI	ACTGGG	2 cut(s)	385, 1488
BpiI	GAAGAC	3 cut(s)	682, 1620, 2832
BpmI	CTGGAG	3 cut(s)	812, 1218, 3224
Bpu10I	CCTNAGC	2 cut(s)	1428, 1695
Bpu1102I	GCTNAGC	1 cut(s)	2767
Bpu14I	TTCGAA	2 cut(s)	130, 233
BpuMI	CCSGG	3 cut(s)	542, 3275, 3276
BsaBI	GATNNNNATC	2 cut(s)	714, 2054
BsaHI	GRCGYC	1 cut(s)	27
BsaXI	ACNNNNNCTCC	2 cut(s)	1747, 1777
Bse1I	ACTGG	6 cut(s)	11, 380, 1483, 2234, 2302, 2509
Bse3DI	GCAATG	2 cut(s)	2700, 3021
Bse8I	GATNNNNATC	2 cut(s)	714, 2054
BseBI	CCWGG	6 cut(s)	215, 675, 791, 1556, 1866, 2734
BseJI	GATNNNNATC	2 cut(s)	714, 2054
BseMI	GCAATG	2 cut(s)	2700, 3021
BseNI	ACTGG	6 cut(s)	11, 380, 1483, 2234, 2302, 2509
BseRI	GAGGAG	5 cut(s)	69, 99, 848, 913, 2059
BseSI	GKGCMC	3 cut(s)	623, 1526, 3223
BseXI	GCAGC	5 cut(s)	32, 35, 102, 1678, 2332
BsgI	GTGCAG	3 cut(s)	289, 635, 1291
BshFI	GGCC	9 cut(s)	621, 839, 1194, 1361, 1754, 1776, 1994, 2718, 3166
BshNI	GGYRCC	7 cut(s)	867, 1130, 1859, 1971, 1985, 2377, 2507
BsiHKAI	GWGCWC	6 cut(s)	229, 1298, 1526, 1809, 3051, 3223
BsiHKCI	CYCGRG	1 cut(s)	3274
BsiSI	CCGG	2 cut(s)	542, 3275
BslFI	GGGAC	4 cut(s)	196, 2037, 3222, 3257
BsmAI	GTCTC	3 cut(s)	788, 1493, 3296
BsmFI	GGGAC	4 cut(s)	196, 2037, 3222, 3257
BsmI	GAATGC	2 cut(s)	1520, 1659
BsnI	GGCC	9 cut(s)	621, 839, 1194, 1361, 1754, 1776, 1994, 2718, 3166
BsoBI	CYCGRG	1 cut(s)	3274
Bsp119I	TTCGAA	2 cut(s)	130, 233
Bsp120I	GGGCCC	1 cut(s)	619
Bsp1286I	GDGCHC	7 cut(s)	229, 623, 1298, 1526, 1809, 3051, 3223
Bsp1720I	GCTNAGC	1 cut(s)	2767
BspACI	CCGC	3 cut(s)	113, 762, 2349
BspANI	GGCC	9 cut(s)	621, 839, 1194, 1361, 1754, 1776, 1994, 2718, 3166
BspMAI	CTGCAG	5 cut(s)	428, 1382, 1449, 2386, 3246
BspMI	ACCTGC	5 cut(s)	476, 1333, 1852, 1903, 1973
BspPI	GGATC	6 cut(s)	1224, 1454, 1467, 2759, 2919, 2977
BspQI	GCTCTTC	2 cut(s)	66, 3275
BspT104I	TTCGAA	2 cut(s)	130, 233
BspT107I	GGYRCC	7 cut(s)	867, 1130, 1859, 1971, 1985, 2377, 2507
BsrBI	CCGCTC	1 cut(s)	115
BsrDI	GCAATG	2 cut(s)	2700, 3021
BsrI	ACTGG	6 cut(s)	11, 380, 1483, 2234, 2302, 2509
BssNI	GRCGYC	1 cut(s)	27
BssT1I	CCWWGG	6 cut(s)	451, 840, 1108, 1989, 2149, 3396
Bst2UI	CCWGG	6 cut(s)	215, 675, 791, 1556, 1866, 2734
Bst6I	CTCTTC	4 cut(s)	66, 1923, 3275, 3312
BstACI	GRCGYC	1 cut(s)	27
BstAPI	GCANNNNNTGC	1 cut(s)	2102
BstBI	TTCGAA	2 cut(s)	130, 233
BstC8I	GCNNGC	4 cut(s)	12, 666, 2382, 3292
BstENI	CCTNNNNNAGG	2 cut(s)	2996, 3330
BstHHI	GCGC	2 cut(s)	513, 1900
BstMAI	GTCTC	3 cut(s)	788, 1493, 3296
BstMWI	GCNNNNNNNGC	8 cut(s)	1339, 1697, 1760, 2102, 2390, 3046, 3172, 3291
BstNI	CCWGG	6 cut(s)	215, 675, 791, 1556, 1866, 2734
BstNSI	RCATGY	4 cut(s)	668, 864, 1417, 2577
BstSCI	CCNGG	9 cut(s)	213, 540, 673, 789, 1554, 1864, 2732, 3273, 3274
BstSFI	CTRYAG	8 cut(s)	424, 1378, 1445, 2161, 2382, 2755, 2993, 3242
BstSLI	GKGCMC	3 cut(s)	623, 1526, 3223
BstV1I	GCAGC	5 cut(s)	32, 35, 102, 1678, 2332
BstV2I	GAAGAC	3 cut(s)	682, 1620, 2832
BstX2I	RGATCY	6 cut(s)	381, 1030, 1216, 1459, 2751, 2924
BstYI	RGATCY	6 cut(s)	381, 1030, 1216, 1459, 2751, 2924
BsuI	GTATCC	1 cut(s)	3329
BsuRI	GGCC	9 cut(s)	621, 839, 1194, 1361, 1754, 1776, 1994, 2718, 3166
BtrI	CACGTC	1 cut(s)	3384
BtsIMutI	CAGTG	4 cut(s)	1005, 2265, 2679, 3267
BveI	ACCTGC	5 cut(s)	476, 1333, 1852, 1903, 1973
Cac8I	GCNNGC	4 cut(s)	12, 666, 2382, 3292
CaiI	CAGNNNCTG	1 cut(s)	1718
CfoI	GCGC	2 cut(s)	513, 1900
Cfr13I	GGNCC	6 cut(s)	183, 619, 620, 1775, 2300, 2493
Cfr9I	CCCGGG	1 cut(s)	3274
CseI	GACGC	2 cut(s)	16, 1967
Csp6I	GTAC	9 cut(s)	4, 858, 868, 1860, 1972, 2528, 2571, 2858, 3105
CviQI	GTAC	9 cut(s)	4, 858, 868, 1860, 1972, 2528, 2571, 2858, 3105
DraI	TTTAAA	1 cut(s)	492
DriI	GACNNNNNGTC	1 cut(s)	3182
EaeI	YGGCCR	1 cut(s)	2716
Eam1104I	CTCTTC	4 cut(s)	66, 1923, 3275, 3312
Eam1105I	GACNNNNNGTC	1 cut(s)	3182
EarI	CTCTTC	4 cut(s)	66, 1923, 3275, 3312
Ecl136II	GAGCTC	2 cut(s)	227, 3049
Eco130I	CCWWGG	6 cut(s)	451, 840, 1108, 1989, 2149, 3396
Eco147I	AGGCCT	3 cut(s)	839, 1194, 3166
Eco24I	GRGCYC	3 cut(s)	229, 623, 3051
Eco47I	GGWCC	3 cut(s)	183, 2300, 2493
Eco53kI	GAGCTC	2 cut(s)	227, 3049
Eco57I	CTGAAG	4 cut(s)	1065, 1512, 1740, 2844
Eco88I	CYCGRG	1 cut(s)	3274
EcoICRI	GAGCTC	2 cut(s)	227, 3049
EcoNI	CCTNNNNNAGG	2 cut(s)	2996, 3330
EcoO109I	RGGNCCY	1 cut(s)	619
EcoRII	CCWGG	6 cut(s)	213, 673, 789, 1554, 1864, 2732
EcoT14I	CCWWGG	6 cut(s)	451, 840, 1108, 1989, 2149, 3396
EcoT22I	ATGCAT	1 cut(s)	1659
EcoT38I	GRGCYC	3 cut(s)	229, 623, 3051
ErhI	CCWWGG	6 cut(s)	451, 840, 1108, 1989, 2149, 3396
FaqI	GGGAC	4 cut(s)	196, 2037, 3222, 3257
FbaI	TGATCA	2 cut(s)	709, 3345
FblI	GTMKAC	1 cut(s)	973
Fnu4HI	GCNGC	6 cut(s)	46, 49, 91, 1692, 2346, 2349
FriOI	GRGCYC	3 cut(s)	229, 623, 3051
Fsp4HI	GCNGC	6 cut(s)	46, 49, 91, 1692, 2346, 2349
FspBI	CTAG	6 cut(s)	323, 1043, 1709, 1743, 1901, 2081
GlaI	GCGC	2 cut(s)	512, 1899
GluI	GCNGC	6 cut(s)	46, 49, 91, 1692, 2346, 2349
GsuI	CTGGAG	3 cut(s)	812, 1218, 3224
HaeIII	GGCC	9 cut(s)	621, 839, 1194, 1361, 1754, 1776, 1994, 2718, 3166
HapII	CCGG	2 cut(s)	542, 3275
HgaI	GACGC	2 cut(s)	16, 1967
HhaI	GCGC	2 cut(s)	513, 1900
Hin1I	GRCGYC	1 cut(s)	27
Hin6I	GCGC	2 cut(s)	511, 1898
HinP1I	GCGC	2 cut(s)	511, 1898
HincII	GTYRAC	1 cut(s)	337
HindII	GTYRAC	1 cut(s)	337
HindIII	AAGCTT	3 cut(s)	851, 1340, 2764
HpaI	GTTAAC	1 cut(s)	337
HpaII	CCGG	2 cut(s)	542, 3275
HphI	GGTGA	5 cut(s)	446, 950, 1314, 1348, 1553
Hpy166II	GTNNAC	9 cut(s)	337, 974, 1311, 1524, 1832, 1938, 2686, 2845, 3221
Hpy8I	GTNNAC	9 cut(s)	337, 974, 1311, 1524, 1832, 1938, 2686, 2845, 3221
Hpy99I	CGWCG	2 cut(s)	32, 817
HpyCH4IV	ACGT	6 cut(s)	333, 687, 890, 1268, 1292, 3383
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1339, 1697, 1760, 2102, 2390, 3046, 3172, 3291
HpySE526I	ACGT	6 cut(s)	333, 687, 890, 1268, 1292, 3383
Hsp92I	GRCGYC	1 cut(s)	27
HspAI	GCGC	2 cut(s)	511, 1898
KpnI	GGTACC	3 cut(s)	871, 1863, 1975
Ksp22I	TGATCA	2 cut(s)	709, 3345
KspAI	GTTAAC	1 cut(s)	337
LguI	GCTCTTC	2 cut(s)	66, 3275
LmnI	GCTCC	5 cut(s)	77, 900, 1768, 1804, 3327
Lsp1109I	GCAGC	5 cut(s)	32, 35, 102, 1678, 2332
LweI	GCATC	4 cut(s)	451, 786, 2596, 3027
MaeI	CTAG	6 cut(s)	323, 1043, 1709, 1743, 1901, 2081
MaeII	ACGT	6 cut(s)	333, 687, 890, 1268, 1292, 3383
MaeIII	GTNAC	8 cut(s)	434, 844, 1003, 1054, 1714, 2328, 2500, 2962
MbiI	CCGCTC	1 cut(s)	115
MflI	RGATCY	6 cut(s)	381, 1030, 1216, 1459, 2751, 2924
MhlI	GDGCHC	7 cut(s)	229, 623, 1298, 1526, 1809, 3051, 3223
MlsI	TGGCCA	1 cut(s)	2718
MluNI	TGGCCA	1 cut(s)	2718
MlyI	GAGTC	6 cut(s)	361, 1114, 1634, 2165, 3222, 3298
MmeI	TCCRAC	3 cut(s)	727, 795, 1903
Mox20I	TGGCCA	1 cut(s)	2718
Mph1103I	ATGCAT	1 cut(s)	1659
MroXI	GAANNNNTTC	2 cut(s)	209, 2796
MscI	TGGCCA	1 cut(s)	2718
MslI	CAYNNNNRTG	2 cut(s)	2112, 2643
Msp20I	TGGCCA	1 cut(s)	2718
MspA1I	CMGCKG	1 cut(s)	292
MspI	CCGG	2 cut(s)	542, 3275
MspR9I	CCNGG	9 cut(s)	215, 542, 675, 791, 1556, 1866, 2734, 3275, 3276
Mva1269I	GAATGC	2 cut(s)	1520, 1659
MvaI	CCWGG	6 cut(s)	215, 675, 791, 1556, 1866, 2734
MwoI	GCNNNNNNNGC	8 cut(s)	1339, 1697, 1760, 2102, 2390, 3046, 3172, 3291
NciI	CCSGG	3 cut(s)	542, 3275, 3276
NmeAIII	GCCGAG	1 cut(s)	2573
NmuCI	GTSAC	3 cut(s)	434, 1003, 2962
NsiI	ATGCAT	1 cut(s)	1659
NspI	RCATGY	4 cut(s)	668, 864, 1417, 2577
NspV	TTCGAA	2 cut(s)	130, 233
PaeI	GCATGC	1 cut(s)	668
PaqCI	CACCTGC	3 cut(s)	1333, 1852, 1973
PasI	CCCWGGG	1 cut(s)	1865
PceI	AGGCCT	3 cut(s)	839, 1194, 3166
PciI	ACATGT	2 cut(s)	860, 2573
PciSI	GCTCTTC	2 cut(s)	66, 3275
PcsI	WCGNNNNNNNCGW	1 cut(s)	136
PctI	GAATGC	2 cut(s)	1520, 1659
PdmI	GAANNNNTTC	2 cut(s)	209, 2796
PfeI	GAWTC	8 cut(s)	136, 550, 1904, 2387, 2398, 2411, 2797, 3157
PflMI	CCANNNNNTGG	3 cut(s)	533, 2189, 2309
PfoI	TCCNGGA	2 cut(s)	213, 789
PkrI	GCNGC	6 cut(s)	47, 50, 92, 1693, 2347, 2350
PleI	GAGTC	6 cut(s)	360, 1114, 1633, 2164, 3221, 3298
PpsI	GAGTC	6 cut(s)	360, 1114, 1633, 2164, 3221, 3298
PscI	ACATGT	2 cut(s)	860, 2573
Psp124BI	GAGCTC	2 cut(s)	229, 3051
Psp6I	CCWGG	6 cut(s)	213, 673, 789, 1554, 1864, 2732
PspGI	CCWGG	6 cut(s)	213, 673, 789, 1554, 1864, 2732
PspOMI	GGGCCC	1 cut(s)	619
PspPI	GGNCC	6 cut(s)	183, 619, 620, 1775, 2300, 2493
PstI	CTGCAG	5 cut(s)	428, 1382, 1449, 2386, 3246
PstNI	CAGNNNCTG	1 cut(s)	1718
PsuI	RGATCY	6 cut(s)	381, 1030, 1216, 1459, 2751, 2924
PvuII	CAGCTG	1 cut(s)	292
RsaI	GTAC	9 cut(s)	5, 859, 869, 1861, 1973, 2529, 2572, 2859, 3106
RsaNI	GTAC	9 cut(s)	4, 858, 868, 1860, 1972, 2528, 2571, 2858, 3105
RseI	CAYNNNNRTG	2 cut(s)	2112, 2643
SacI	GAGCTC	2 cut(s)	229, 3051
SapI	GCTCTTC	2 cut(s)	66, 3275
SatI	GCNGC	6 cut(s)	46, 49, 91, 1692, 2346, 2349
Sau96I	GGNCC	6 cut(s)	183, 619, 620, 1775, 2300, 2493
SchI	GAGTC	6 cut(s)	361, 1114, 1634, 2165, 3222, 3298
ScrFI	CCNGG	9 cut(s)	215, 542, 675, 791, 1556, 1866, 2734, 3275, 3276
SduI	GDGCHC	7 cut(s)	229, 623, 1298, 1526, 1809, 3051, 3223
SfaNI	GCATC	4 cut(s)	451, 786, 2596, 3027
SfcI	CTRYAG	8 cut(s)	424, 1378, 1445, 2161, 2382, 2755, 2993, 3242
SfuI	TTCGAA	2 cut(s)	130, 233
SinI	GGWCC	3 cut(s)	183, 2300, 2493
SmaI	CCCGGG	1 cut(s)	3276
SmiMI	CAYNNNNRTG	2 cut(s)	2112, 2643
SphI	GCATGC	1 cut(s)	668
SseBI	AGGCCT	3 cut(s)	839, 1194, 3166
SsiI	CCGC	3 cut(s)	113, 762, 2349
SspI	AATATT	2 cut(s)	1407, 1791
SspMI	CTAG	6 cut(s)	323, 1043, 1709, 1743, 1901, 2081
SstI	GAGCTC	2 cut(s)	229, 3051
StuI	AGGCCT	3 cut(s)	839, 1194, 3166
StyD4I	CCNGG	9 cut(s)	213, 540, 673, 789, 1554, 1864, 2732, 3273, 3274
StyI	CCWWGG	6 cut(s)	451, 840, 1108, 1989, 2149, 3396
TaiI	ACGT	6 cut(s)	336, 690, 893, 1271, 1295, 3386
TaqI	TCGA	8 cut(s)	130, 233, 1398, 1662, 2168, 2396, 2414, 2814
TaqII	GACCGA	1 cut(s)	2481
TatI	WGTACW	2 cut(s)	3, 2527
TauI	GCSGC	1 cut(s)	2351
TfiI	GAWTC	8 cut(s)	136, 550, 1904, 2387, 2398, 2411, 2797, 3157
TscAI	CASTG	4 cut(s)	1012, 2272, 2686, 3274
TseFI	GTSAC	3 cut(s)	434, 1003, 2962
TseI	GCWGC	5 cut(s)	45, 48, 90, 1691, 2345
Tsp45I	GTSAC	3 cut(s)	434, 1003, 2962
TspGWI	ACGGA	3 cut(s)	595, 3206, 3209
TspMI	CCCGGG	1 cut(s)	3274
TspRI	CASTG	4 cut(s)	1012, 2272, 2686, 3274
Van91I	CCANNNNNTGG	3 cut(s)	533, 2189, 2309
VneI	GTGCAC	2 cut(s)	1522, 3219
VpaK11BI	GGWCC	3 cut(s)	183, 2300, 2493
XagI	CCTNNNNNAGG	2 cut(s)	2996, 3330
XapI	RAATTY	3 cut(s)	932, 2487, 3373
XbaI	TCTAGA	2 cut(s)	1042, 1742
XceI	RCATGY	4 cut(s)	668, 864, 1417, 2577
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1948, 3394
XmaI	CCCGGG	1 cut(s)	3274
XmiI	GTMKAC	1 cut(s)	973
XmnI	GAANNNNTTC	2 cut(s)	209, 2796
XspI	CTAG	6 cut(s)	323, 1043, 1709, 1743, 1901, 2081
Zsp2I	ATGCAT	1 cut(s)	1659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.