Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0009572

Physical Location & Seq

Genomic Coordinates Forward (+)

8828356 .. 8839860

Gene Structure 11505 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG057100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3426 bp

ATGTATTGGCTGGCCACTCGAAACGGCGTCGTCTGCCGTCCTCTGGCTCTTCTTCTCCGCTCCCCTCCTCGTAAATGCAGCTCCGTCAGCCCCTCTCCGATGCTCATTGGACAGTTTCGGAGGATTCATTGTTTCAAAGACCAAAAGAGTTTACGAGGAAGCAGAAAAACTACCAAGAAACTTAATGCCTTGAATGATTTCCTGGATGAGAGAGCTGTTTCTAACATATTGTGGTGGAAGGAGAGGATGCAGTTGTGCAGGAAGCCTTCAACTGTCCAGCTGGTGAAAAGGCTTGATTATTCTAATTTGCTAGGCTTGGATGTCAACTTAAAAAATGGGAGTCTGAAAGAGGGAACACTCAATTCGGAGATCTTGCAGTTCAAGTCAAAGTTTCCACGTGAAGTTTTGCTCTGCAGAGTTGGTGACTTTTATGAAGCCTTAGGAATAGATGCTTGTGTATTGGTTGAATATGCAGGTTTGAATCCTTTTGGGGGTCTGCGTTCAGATAGTATCCCAAGAGCTGGTTGCCCAGTTGTGAATCTTCGACAAACTTTGGATGATCTGACACGGAATGGGTTTTCAGTGTGCATAGTGGAAGAAGTTCAGGGTCCAACACAAGCGCGTTCCCGTAAAGGTCGTTTTATATCTGGGCATGCACATCCCGGTAGTCCTTACGTTTTCGGACTTGTTGGGGTCGATCACGATCTTGACTTTCCAGAACCAATGCCTGTAGTTGGAATATCTCGTTCTGCTAGAGGTTATTGCATCAACTTTGTATTGGAAACCATGAAAACTTATTCGTCAGAGGATGGTTTGACTGAAGAAGCCTTGGTTGCTAAGCTCCGTACTTGTCGGTACCATCATTTATTCCTGCATACATCATTGAGGAACAATTTCTCAGGCACTTGTCGTTGGGGTGAATTTGGTGAGGGAGGCCTTCTATGGGGAGAATGTAGCGGTAGGCATTTTGAATGGTTTGAAGGCAATCCTGTGTTTGAGCTTTTGTCTAAGGTTAGAGATTTATATGGTCTTGATAATGAAGTTACATTTAGGAATGTCACTGTGTCTTCAGAAAACAGACCCCAGCCCCTATCCCTAGGAACAGCGACTCAAATTGGTGCCATACCAACTGAGGGAATACCTTGTTTGTTGAAGGTGTTGCTTCCTTCAAACTGCTCAGGCCTACCTGTACTGTATGTTAGAGATCTTCTTCTTAATCCTCCTCCTTATGATATATCTTCCACAATTCAAGCAACATGCAGACTTATGAGCAGCATTGCTTGCTCGATTCCTGAGTTCACTTGTGTTTCACCTGCAAAGCTGGTGAAACTTCTGGAACTCAGGGAGGCCAATCATATTGAGTTTTGCAGAATTAAAAATGTTCTCGATGAAATACTGCACATGCATAAAAATCCTGAGCTGGGTAAGATTCTGAAGTTGTTGATGGATCCTACATGGGTGGCGACTGGGTTGAAAATTGACTTTGAGACATTAGTTAATGAATGTGAATGCGCTTCTGATAGAATTGGTGAAATGATCTCCCTGGATGGTGAACATGATCAAAACCTTAGTTCCTTTCCTGTTGTTCCTAGCGATTTTTTCGAGGATATGGAGTGTTCTTGGAAGGGTCGTATAAAGAGAATCCATATAGAGGAGGCATTTGCAGAAGTGGAAAAGGCTGCTGAGGCCTTATCCCTAGCAGTCACTGAAGATTTCATGCCCATTGTATCTAGAATAAAGGCTACCACAGCTCCACTTGGAGGCCCGAAGGGGGAAATATTATATGCGCGGGAGCATGAAGCTGTTTGGTTTAAGGGAAAACGATTTGCTCCAGCTGTTTGGGCTGGCACCCCTGGGGAAGAACAAATTAAACAGCTTAAACCTGCTTTAGATTCAAAAGGTAGAAAGGTCGGAGAAGAGTGGTTTACTACAATGAAAGTGGAGGATGCTTTAACAAGGTACCATGAAGCAGGGGCGAAAGCAAAAACAAGGGTTTTGGAGTTGTTGAGAGAACTTTCTTCTGACTTACAAGCAAAGATCAATATCCTTGTCTTTGCCTCAATGCTTCTAGTTATTGCAAAGGCATTATTTGCTCATGTGAGTGAAGGGAGAAGAAGGAAATGGGTTTTTCCTGCCCTTGGAGAGTCCAGTTGGTCTAAGCATTGGACGCAGAATGTGAAACCATTGAGGGGAGAAAATAGAATGGAGATAGTTGGTCTATCACCATACTGGTTGGATGTAGCAGAAGGCAGCGCTGTAAATAACACTGTGGATATGCAATCATTGTTCCTTTTAACTGGACCCAATGGTGGTGGTAAATCAAGTCTGCTACGATCAATTTGTGCTGCTGCATTACTTGGAGTTTGTGGGTTTATGGTGCCTGCACAGTCTGCCTCGATTCCTCATTTTGATTCTATTATGCTTCACATGAAATCTTATGACAGCCCCGCTGATGGGAAAAGCTCCTTTCAGGTGGAAATGGCAGAAATTCGGTCTATTGTTTCTGGAGCCACTAAAAGAAGCCTTGTACTTGTAGATGAAATTTGTCGAGGAACAGAAACAGCTAAGGGAACATGTATTGCTGGTAGTATTGTTGAAACTCTTGATGCAATAGGATGTCTTGGTATCATATCTACTCACTTGCACGGGATATTTAGCTTGCCTCTTAATACAAAGAATACGGTGCATAAAGCAATGGGAACTGTATATGTTGATGGACAACCAAAACCAACCTGGAAATTGATGGACGGCATATGTAGAGAAAGCCTTGCATTTGAAACGGCTAAGAAGGAAGGAATTCCTGAAACAATAATTGAAAGAGCTGAAGGCCTGTACCATTCAGTTTATGCGAAGGAGGTGATTCGAGGAAAGATTGACTCAAAACTAGAAGAGTTCTGTTCCACAGGTTTTAATAATTTTGATAGGTCTCATCCTCAATCAAGTAGTCCAAGAGTTGAAGCTGTCGATGGGACAGGATCAGTGAACAATATGGAAGTCTTACAGAAGGAGGTTGAGAGTGCAATCACCTTGATTTGCCAAAAGAAGCTGATTGAGCTCGACAACGAAAAGACATCAGAACTTACTGATATACAGTGTGTTCCAATTCATGTCAGGGAACAGCCACCTCCATCTACCGTAGGTGCTTCATGTGTGTATGTGATTTTCAGACCTGATAGGAAACTATATGTTGGACAGACGGATGATTTGGAGGGTCGGGTGCGCACACATCGTTCAAAGGAAGGAATGCAAAAGGCAAATTTTCTTTATTTCACTGTCCCGGGGAAGAGCTTGGCTTGCCAATTAGAGACTCTTCTCATCAACCAGCTCCCTAATCAAGGCTTCCATCTCAGTAATGTGGCTGATGGTAAACATAGGAATTTTGGCACCTCCAACATTTCCTTGGAAGGTGTGAAAGTTTGTCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000002 GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006281 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007005 GO:0008094 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009507 GO:0009536 GO:0009579 GO:0009628 GO:0009987 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032042 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034357 GO:0034641 GO:0042623 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044436 GO:0044444 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GIY-YIG MutS_I MutS_V

Protein Analysis

1141

Amino Acids

126.57

Weight (kDa)

7.65

Isoelectric Point (pI)

40.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	125 - 215	8.6e-10	MutS domain I
MutS_V	PF00488	764 - 946	3.7e-40	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24320 AT3G24320
fragaria_vesca	FvH4_4g05000 FvH4_4g05000 FvH4_4g05000
malus_domestica	MD13G1223200.v1.1
prunus_persica	Prupe.1G050200_v2.0.a1
pyrus_communis	pycom13g19710
rosa_chinensis	RchiOBHm_Chr4g0395811
rosa_laevigata	RLG00000009591
rosa_multiflora	Rmu_sc0001103.1_g000036 Rmu_sc0008803.1_g000001
rosa_roxburghii	Rroxscaffold_5G00340650
rosa_rugosa	Rorug03G0354100 Rorug03G0354200
rosa_samantha	Rh4AG062500 Rh4AG063500 Rh4AG067100 Rh4BG062200 Rh4CG068500 Rh4DG057100
rosa_wichuraiana	Rw4G005140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1321
Acc16I	TGCGCA	1 cut(s)	3221
Acc36I	ACCTGC	3 cut(s)	464, 1321, 1891
Acc65I	GGTACC	2 cut(s)	855, 1959
AccB1I	GGYRCC	6 cut(s)	855, 1118, 1847, 1959, 2377, 3383
AccB7I	CCANNNNNTGG	1 cut(s)	521
AccBSI	CCGCTC	1 cut(s)	60
AccII	CGCG	2 cut(s)	622, 1789
AciI	CCGC	4 cut(s)	58, 957, 1789, 2448
AclWI	GGATC	3 cut(s)	1442, 1455, 2983
AcoI	YGGCCR	1 cut(s)	12
AcsI	RAATTY	6 cut(s)	920, 2487, 2541, 2796, 3256, 3376
AcuI	CTGAAG	5 cut(s)	840, 1053, 1454, 1728, 2844
AcvI	CACGTG	1 cut(s)	398
AcyI	GRCGYC	1 cut(s)	27
AfaI	GTAC	6 cut(s)	847, 857, 1191, 1961, 2529, 2834
AfeI	AGCGCT	1 cut(s)	2254
AflIII	ACRYGT	1 cut(s)	2573
AjnI	CCWGG	4 cut(s)	201, 1542, 1852, 2732
Alw21I	GWGCWC	1 cut(s)	3057
Alw26I	GTCTC	3 cut(s)	1481, 2931, 3299
AlwI	GGATC	3 cut(s)	1442, 1455, 2983
AlwNI	CAGNNNCTG	1 cut(s)	1706
Ama87I	CYCGRG	1 cut(s)	3277
Aor51HI	AGCGCT	1 cut(s)	2254
AoxI	GGCC	7 cut(s)	12, 934, 1180, 1347, 1686, 1762, 2827
ApeKI	GCWGC	6 cut(s)	78, 1272, 1679, 2250, 2345, 2348
ApoI	RAATTY	6 cut(s)	920, 2487, 2541, 2796, 3256, 3376
ArsI	GACNNNNNNTTYG	2 cut(s)	3187, 3219
Asp700I	GAANNNNTTC	4 cut(s)	197, 600, 893, 2796
Asp718I	GGTACC	2 cut(s)	855, 1959
AspA2I	CCTAGG	1 cut(s)	1096
AspLEI	GCGC	5 cut(s)	622, 1514, 1789, 2255, 3222
AspS9I	GGNCC	3 cut(s)	608, 1763, 2300
AsuC2I	CCSGG	3 cut(s)	663, 3278, 3279
AvaI	CYCGRG	1 cut(s)	3277
AvaII	GGWCC	2 cut(s)	608, 2300
AvrII	CCTAGG	1 cut(s)	1096
AxyI	CCTNAGG	1 cut(s)	439
BalI	TGGCCA	1 cut(s)	14
BamHI	GGATCC	1 cut(s)	1447
BanI	GGYRCC	6 cut(s)	855, 1118, 1847, 1959, 2377, 3383
BanII	GRGCYC	1 cut(s)	3057
BbrPI	CACGTG	1 cut(s)	398
BbsI	GAAGAC	1 cut(s)	1059
Bbv12I	GWGCWC	1 cut(s)	3057
BbvCI	CCTCAGC	1 cut(s)	1683
BbvI	GCAGC	6 cut(s)	90, 1284, 1666, 2262, 2332, 2335
BceAI	ACGGC	4 cut(s)	21, 40, 2764, 2796
BciT130I	CCWGG	4 cut(s)	203, 1544, 1854, 2734
BciVI	GTATCC	1 cut(s)	521
BclI	TGATCA	1 cut(s)	1558
BcnI	CCSGG	3 cut(s)	663, 3278, 3279
BcoDI	GTCTC	3 cut(s)	1481, 2931, 3299
BfaI	CTAG	8 cut(s)	311, 753, 1097, 1590, 1697, 1731, 2069, 2885
BfmI	CTRYAG	2 cut(s)	412, 729
BfoI	RGCGCY	1 cut(s)	2256
BfuAI	ACCTGC	3 cut(s)	464, 1321, 1891
BfuI	GTATCC	1 cut(s)	521
BglII	AGATCT	2 cut(s)	369, 1204
BisI	GCNGC	6 cut(s)	79, 1273, 1680, 2251, 2346, 2349
BlnI	CCTAGG	1 cut(s)	1096
BlpI	GCTNAGC	1 cut(s)	837
BlsI	GCNGC	6 cut(s)	80, 1274, 1681, 2252, 2347, 2350
Bme1390I	CCNGG	7 cut(s)	203, 663, 1544, 1854, 2734, 3278, 3279
Bme18I	GGWCC	2 cut(s)	608, 2300
BmeT110I	CYCGRG	1 cut(s)	3277
BmgT120I	GGNCC	3 cut(s)	608, 1763, 2300
BmrFI	CCNGG	7 cut(s)	203, 663, 1544, 1854, 2734, 3278, 3279
BmrI	ACTGGG	2 cut(s)	524, 1476
BmsI	GCATC	6 cut(s)	90, 237, 439, 774, 1936, 2596
BmuI	ACTGGG	2 cut(s)	524, 1476
BpiI	GAAGAC	1 cut(s)	1059
BpmI	CTGGAG	2 cut(s)	1815, 2526
Bpu10I	CCTNAGC	4 cut(s)	1177, 1416, 1683, 2565
Bpu1102I	GCTNAGC	1 cut(s)	837
BpuMI	CCSGG	3 cut(s)	663, 3278, 3279
BsaAI	YACGTR	1 cut(s)	398
BsaBI	GATNNNNATC	2 cut(s)	702, 2042
BsaHI	GRCGYC	1 cut(s)	27
BsaI	GGTCTC	1 cut(s)	2931
BsaJI	CCNNGG	9 cut(s)	828, 1096, 1542, 1852, 1853, 2137, 3277, 3278, 3399
BsaXI	ACNNNNNCTCC	2 cut(s)	1735, 1765
Bse1I	ACTGG	5 cut(s)	530, 1471, 2148, 2234, 2302
Bse21I	CCTNAGG	1 cut(s)	439
Bse3DI	GCAATG	2 cut(s)	1275, 2700
Bse8I	GATNNNNATC	2 cut(s)	702, 2042
BseBI	CCWGG	4 cut(s)	203, 1544, 1854, 2734
BseDI	CCNNGG	9 cut(s)	828, 1096, 1542, 1852, 1853, 2137, 3277, 3278, 3399
BseJI	GATNNNNATC	2 cut(s)	702, 2042
BseMI	GCAATG	2 cut(s)	1275, 2700
BseMII	CTCAG	8 cut(s)	912, 1122, 1191, 1284, 1354, 1407, 1674, 3361
BseNI	ACTGG	5 cut(s)	530, 1471, 2148, 2234, 2302
BseRI	GAGGAG	3 cut(s)	57, 1212, 1667
BseXI	GCAGC	6 cut(s)	90, 1284, 1666, 2262, 2332, 2335
BseYI	CCCAGC	2 cut(s)	1083, 1420
BsgI	GTGCAG	3 cut(s)	277, 1382, 2367
Bsh1236I	CGCG	2 cut(s)	622, 1789
BshFI	GGCC	7 cut(s)	14, 936, 1182, 1349, 1688, 1764, 2829
BshNI	GGYRCC	6 cut(s)	855, 1118, 1847, 1959, 2377, 3383
BsiHKAI	GWGCWC	1 cut(s)	3057
BsiHKCI	CYCGRG	1 cut(s)	3277
BsiSI	CCGG	2 cut(s)	663, 3278
BslFI	GGGAC	2 cut(s)	2983, 3260
BsmAI	GTCTC	3 cut(s)	1481, 2931, 3299
BsmFI	GGGAC	2 cut(s)	2983, 3260
BsmI	GAATGC	2 cut(s)	1514, 3249
BsnI	GGCC	7 cut(s)	14, 936, 1182, 1349, 1688, 1764, 2829
Bso31I	GGTCTC	1 cut(s)	2931
BsoBI	CYCGRG	1 cut(s)	3277
Bsp1286I	GDGCHC	1 cut(s)	3057
Bsp1720I	GCTNAGC	1 cut(s)	837
BspACI	CCGC	4 cut(s)	58, 957, 1789, 2448
BspANI	GGCC	7 cut(s)	14, 936, 1182, 1349, 1688, 1764, 2829
BspCNI	CTCAG	8 cut(s)	911, 1123, 1190, 1285, 1353, 1408, 1675, 3360
BspFNI	CGCG	2 cut(s)	622, 1789
BspMAI	CTGCAG	1 cut(s)	416
BspMI	ACCTGC	3 cut(s)	464, 1321, 1891
BspPI	GGATC	3 cut(s)	1442, 1455, 2983
BspQI	GCTCTTC	2 cut(s)	54, 3278
BspT107I	GGYRCC	6 cut(s)	855, 1118, 1847, 1959, 2377, 3383
BspTNI	GGTCTC	1 cut(s)	2931
BsrBI	CCGCTC	1 cut(s)	60
BsrDI	GCAATG	2 cut(s)	1275, 2700
BsrI	ACTGG	5 cut(s)	530, 1471, 2148, 2234, 2302
BssECI	CCNNGG	9 cut(s)	828, 1096, 1542, 1852, 1853, 2137, 3277, 3278, 3399
BssNI	GRCGYC	1 cut(s)	27
BssT1I	CCWWGG	4 cut(s)	828, 1096, 2137, 3399
Bst2UI	CCWGG	4 cut(s)	203, 1544, 1854, 2734
Bst6I	CTCTTC	5 cut(s)	54, 1911, 2883, 3278, 3315
BstACI	GRCGYC	1 cut(s)	27
BstAPI	GCANNNNNTGC	3 cut(s)	1281, 2090, 2390
BstBAI	YACGTR	1 cut(s)	398
BstC8I	GCNNGC	7 cut(s)	12, 654, 1282, 1846, 2382, 2660, 3295
BstENI	CCTNNNNNAGG	1 cut(s)	3333
BstFNI	CGCG	2 cut(s)	622, 1789
BstH2I	RGCGCY	1 cut(s)	2256
BstHHI	GCGC	5 cut(s)	622, 1514, 1789, 2255, 3222
BstMAI	GTCTC	3 cut(s)	1481, 2931, 3299
BstNI	CCWGG	4 cut(s)	203, 1544, 1854, 2734
BstNSI	RCATGY	4 cut(s)	656, 1260, 1405, 2577
BstSCI	CCNGG	7 cut(s)	201, 661, 1542, 1852, 2732, 3276, 3277
BstSFI	CTRYAG	2 cut(s)	412, 729
BstUI	CGCG	2 cut(s)	622, 1789
BstV1I	GCAGC	6 cut(s)	90, 1284, 1666, 2262, 2332, 2335
BstV2I	GAAGAC	1 cut(s)	1059
BstX2I	RGATCY	3 cut(s)	369, 1204, 1447
BstXI	CCANNNNNNTGG	1 cut(s)	1839
BstYI	RGATCY	3 cut(s)	369, 1204, 1447
Bsu36I	CCTNAGG	1 cut(s)	439
BsuI	GTATCC	1 cut(s)	521
BsuRI	GGCC	7 cut(s)	14, 936, 1182, 1349, 1688, 1764, 2829
BtsIMutI	CAGTG	7 cut(s)	588, 1059, 1704, 2265, 2985, 3098, 3270
BveI	ACCTGC	3 cut(s)	464, 1321, 1891
Cac8I	GCNNGC	7 cut(s)	12, 654, 1282, 1846, 2382, 2660, 3295
CaiI	CAGNNNCTG	1 cut(s)	1706
CfoI	GCGC	5 cut(s)	622, 1514, 1789, 2255, 3222
Cfr13I	GGNCC	3 cut(s)	608, 1763, 2300
Cfr9I	CCCGGG	1 cut(s)	3277
CseI	GACGC	2 cut(s)	16, 2176
Csp6I	GTAC	6 cut(s)	846, 856, 1190, 1960, 2528, 2833
CspCI	CAANNNNNGTGG	4 cut(s)	1920, 1955, 2293, 2328
CviQI	GTAC	6 cut(s)	846, 856, 1190, 1960, 2528, 2833
EaeI	YGGCCR	1 cut(s)	12
Eam1104I	CTCTTC	5 cut(s)	54, 1911, 2883, 3278, 3315
EarI	CTCTTC	5 cut(s)	54, 1911, 2883, 3278, 3315
Ecl136II	GAGCTC	1 cut(s)	3055
Eco130I	CCWWGG	4 cut(s)	828, 1096, 2137, 3399
Eco147I	AGGCCT	4 cut(s)	936, 1182, 1688, 2829
Eco24I	GRGCYC	1 cut(s)	3057
Eco31I	GGTCTC	1 cut(s)	2931
Eco47I	GGWCC	2 cut(s)	608, 2300
Eco47III	AGCGCT	1 cut(s)	2254
Eco53kI	GAGCTC	1 cut(s)	3055
Eco57I	CTGAAG	5 cut(s)	840, 1053, 1454, 1728, 2844
Eco72I	CACGTG	1 cut(s)	398
Eco81I	CCTNAGG	1 cut(s)	439
Eco88I	CYCGRG	1 cut(s)	3277
EcoICRI	GAGCTC	1 cut(s)	3055
EcoNI	CCTNNNNNAGG	1 cut(s)	3333
EcoRI	GAATTC	1 cut(s)	2796
EcoRII	CCWGG	4 cut(s)	201, 1542, 1852, 2732
EcoT14I	CCWWGG	4 cut(s)	828, 1096, 2137, 3399
EcoT22I	ATGCAT	1 cut(s)	1407
EcoT38I	GRGCYC	1 cut(s)	3057
ErhI	CCWWGG	4 cut(s)	828, 1096, 2137, 3399
FaqI	GGGAC	2 cut(s)	2983, 3260
FauI	CCCGC	2 cut(s)	1782, 2455
FauNDI	CATATG	1 cut(s)	2753
FbaI	TGATCA	1 cut(s)	1558
Fnu4HI	GCNGC	6 cut(s)	79, 1273, 1680, 2251, 2346, 2349
FriOI	GRGCYC	1 cut(s)	3057
Fsp4HI	GCNGC	6 cut(s)	79, 1273, 1680, 2251, 2346, 2349
FspAI	RTGCGCAY	1 cut(s)	3221
FspBI	CTAG	8 cut(s)	311, 753, 1097, 1590, 1697, 1731, 2069, 2885
FspI	TGCGCA	1 cut(s)	3221
GlaI	GCGC	5 cut(s)	621, 1513, 1788, 2254, 3221
GluI	GCNGC	6 cut(s)	79, 1273, 1680, 2251, 2346, 2349
GsaI	CCCAGC	2 cut(s)	1087, 1424
GsuI	CTGGAG	2 cut(s)	1815, 2526
HaeII	RGCGCY	1 cut(s)	2256
HaeIII	GGCC	7 cut(s)	14, 936, 1182, 1349, 1688, 1764, 2829
HapII	CCGG	2 cut(s)	663, 3278
HgaI	GACGC	2 cut(s)	16, 2176
HhaI	GCGC	5 cut(s)	622, 1514, 1789, 2255, 3222
Hin1I	GRCGYC	1 cut(s)	27
Hin6I	GCGC	5 cut(s)	620, 1512, 1787, 2253, 3220
HinP1I	GCGC	5 cut(s)	620, 1512, 1787, 2253, 3220
HincII	GTYRAC	1 cut(s)	325
HindII	GTYRAC	1 cut(s)	325
HpaII	CCGG	2 cut(s)	663, 3278
Hpy166II	GTNNAC	7 cut(s)	152, 325, 1299, 1553, 1926, 2983, 3368
Hpy8I	GTNNAC	7 cut(s)	152, 325, 1299, 1553, 1926, 2983, 3368
Hpy99I	CGWCG	1 cut(s)	32
HpyCH4IV	ACGT	2 cut(s)	397, 675
HpySE526I	ACGT	2 cut(s)	397, 675
Hsp92I	GRCGYC	1 cut(s)	27
HspAI	GCGC	5 cut(s)	620, 1512, 1787, 2253, 3220
KpnI	GGTACC	2 cut(s)	859, 1963
Ksp22I	TGATCA	1 cut(s)	1558
LguI	GCTCTTC	2 cut(s)	54, 3278
LmnI	GCTCC	9 cut(s)	65, 86, 846, 1756, 1792, 1834, 2468, 2507, 3330
Lsp1109I	GCAGC	6 cut(s)	90, 1284, 1666, 2262, 2332, 2335
LweI	GCATC	6 cut(s)	90, 237, 439, 774, 1936, 2596
MaeI	CTAG	8 cut(s)	311, 753, 1097, 1590, 1697, 1731, 2069, 2885
MaeII	ACGT	2 cut(s)	397, 675
MaeIII	GTNAC	4 cut(s)	422, 1042, 1057, 1702
MbiI	CCGCTC	1 cut(s)	60
MflI	RGATCY	3 cut(s)	369, 1204, 1447
MhlI	GDGCHC	1 cut(s)	3057
MlsI	TGGCCA	1 cut(s)	14
MluNI	TGGCCA	1 cut(s)	14
MlyI	GAGTC	5 cut(s)	349, 1102, 2153, 2870, 3301
MmeI	TCCRAC	6 cut(s)	635, 715, 1891, 2214, 3169, 3414
Mox20I	TGGCCA	1 cut(s)	14
Mph1103I	ATGCAT	1 cut(s)	1407
MroXI	GAANNNNTTC	4 cut(s)	197, 600, 893, 2796
MscI	TGGCCA	1 cut(s)	14
MslI	CAYNNNNRTG	1 cut(s)	2100
Msp20I	TGGCCA	1 cut(s)	14
MspA1I	CMGCKG	3 cut(s)	280, 1835, 2450
MspI	CCGG	2 cut(s)	663, 3278
MspR9I	CCNGG	7 cut(s)	203, 663, 1544, 1854, 2734, 3278, 3279
Mva1269I	GAATGC	2 cut(s)	1514, 3249
MvaI	CCWGG	4 cut(s)	203, 1544, 1854, 2734
MvnI	CGCG	2 cut(s)	622, 1789
NciI	CCSGG	3 cut(s)	663, 3278, 3279
NdeI	CATATG	1 cut(s)	2753
NmuCI	GTSAC	3 cut(s)	422, 1057, 1702
NsbI	TGCGCA	1 cut(s)	3221
NsiI	ATGCAT	1 cut(s)	1407
NspI	RCATGY	4 cut(s)	656, 1260, 1405, 2577
PaeI	GCATGC	1 cut(s)	656
PaqCI	CACCTGC	1 cut(s)	1321
PasI	CCCWGGG	1 cut(s)	1853
PceI	AGGCCT	4 cut(s)	936, 1182, 1688, 2829
PciI	ACATGT	1 cut(s)	2573
PciSI	GCTCTTC	2 cut(s)	54, 3278
PctI	GAATGC	2 cut(s)	1514, 3249
PdmI	GAANNNNTTC	4 cut(s)	197, 600, 893, 2796
PflMI	CCANNNNNTGG	1 cut(s)	521
PfoI	TCCNGGA	1 cut(s)	201
PkrI	GCNGC	6 cut(s)	80, 1274, 1681, 2252, 2347, 2350
PleI	GAGTC	5 cut(s)	348, 1102, 2152, 2870, 3301
PmaCI	CACGTG	1 cut(s)	398
PmlI	CACGTG	1 cut(s)	398
PpsI	GAGTC	5 cut(s)	348, 1102, 2152, 2870, 3301
Ppu21I	YACGTR	1 cut(s)	398
PscI	ACATGT	1 cut(s)	2573
Psp124BI	GAGCTC	1 cut(s)	3057
Psp6I	CCWGG	4 cut(s)	201, 1542, 1852, 2732
PspCI	CACGTG	1 cut(s)	398
PspFI	CCCAGC	2 cut(s)	1083, 1420
PspGI	CCWGG	4 cut(s)	201, 1542, 1852, 2732
PspPI	GGNCC	3 cut(s)	608, 1763, 2300
PstI	CTGCAG	1 cut(s)	416
PstNI	CAGNNNCTG	1 cut(s)	1706
PsuI	RGATCY	3 cut(s)	369, 1204, 1447
PvuII	CAGCTG	2 cut(s)	280, 1835
RsaI	GTAC	6 cut(s)	847, 857, 1191, 1961, 2529, 2834
RsaNI	GTAC	6 cut(s)	846, 856, 1190, 1960, 2528, 2833
RseI	CAYNNNNRTG	1 cut(s)	2100
SacI	GAGCTC	1 cut(s)	3057
SapI	GCTCTTC	2 cut(s)	54, 3278
SatI	GCNGC	6 cut(s)	79, 1273, 1680, 2251, 2346, 2349
Sau96I	GGNCC	3 cut(s)	608, 1763, 2300
SchI	GAGTC	5 cut(s)	349, 1102, 2153, 2870, 3301
ScrFI	CCNGG	7 cut(s)	203, 663, 1544, 1854, 2734, 3278, 3279
SduI	GDGCHC	1 cut(s)	3057
SfaNI	GCATC	6 cut(s)	90, 237, 439, 774, 1936, 2596
SfcI	CTRYAG	2 cut(s)	412, 729
SinI	GGWCC	2 cut(s)	608, 2300
SmaI	CCCGGG	1 cut(s)	3279
SmiMI	CAYNNNNRTG	1 cut(s)	2100
SphI	GCATGC	1 cut(s)	656
SseBI	AGGCCT	4 cut(s)	936, 1182, 1688, 2829
SsiI	CCGC	4 cut(s)	58, 957, 1789, 2448
SspI	AATATT	1 cut(s)	1779
SspMI	CTAG	8 cut(s)	311, 753, 1097, 1590, 1697, 1731, 2069, 2885
SstI	GAGCTC	1 cut(s)	3057
StuI	AGGCCT	4 cut(s)	936, 1182, 1688, 2829
StyD4I	CCNGG	7 cut(s)	201, 661, 1542, 1852, 2732, 3276, 3277
StyI	CCWWGG	4 cut(s)	828, 1096, 2137, 3399
TaiI	ACGT	2 cut(s)	400, 678
TaqII	GACCGA	1 cut(s)	2481
TatI	WGTACW	2 cut(s)	1189, 2527
TscAI	CASTG	7 cut(s)	588, 1066, 1711, 2272, 2985, 3098, 3277
TseFI	GTSAC	3 cut(s)	422, 1057, 1702
TseI	GCWGC	6 cut(s)	78, 1272, 1679, 2250, 2345, 2348
Tsp45I	GTSAC	3 cut(s)	422, 1057, 1702
TspGWI	ACGGA	4 cut(s)	73, 583, 833, 3212
TspMI	CCCGGG	1 cut(s)	3277
TspRI	CASTG	7 cut(s)	588, 1066, 1711, 2272, 2985, 3098, 3277
Van91I	CCANNNNNTGG	1 cut(s)	521
VpaK11BI	GGWCC	2 cut(s)	608, 2300
XagI	CCTNNNNNAGG	1 cut(s)	3333
XapI	RAATTY	6 cut(s)	920, 2487, 2541, 2796, 3256, 3376
XbaI	TCTAGA	1 cut(s)	1730
XceI	RCATGY	4 cut(s)	656, 1260, 1405, 2577
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2730, 3397
XmaI	CCCGGG	1 cut(s)	3277
XmaJI	CCTAGG	1 cut(s)	1096
XmnI	GAANNNNTTC	4 cut(s)	197, 600, 893, 2796
XspI	CTAG	8 cut(s)	311, 753, 1097, 1590, 1697, 1731, 2069, 2885
Zsp2I	ATGCAT	1 cut(s)	1407

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.