Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006121

Physical Location & Seq

Genomic Coordinates Reverse (-)

24878941 .. 24892085

Gene Structure 13145 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1240300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2649 bp

ATGTGGTTTTCGTTTTGGAAGTCCAGGGATCGCTTCTCCTTAGACGAGCTCAGGATGATTTTTTATCACTGGTGTAGCATGACGAGGAGGGGCGCAGGCCCCTCTAGGTATTTAACTGATCAGCTGATAAAAGTTCAAATTGTGAATGAAGTAAACAAGGATTTCGTTATTGAGGCGCTGAGATCCATTGCGGAGTTAATAACGTATGGAGACCAGCATGACTCCACCTTTTTTGAATTTTTCATGGAGAAGCAAGTCATGGGAGAGTTTGTACGTATATTGAAGGTTAGCAGAACTATGACTGTGTCACTTCAGTTACTACAGACAATCAGCATTATGATCCAGAACCTAAAAAATGAACACGCTATATACTACATGTTCAGTAATGAACATATGAACTACTTGATAACGTATTCATTTGACTTCCAAAATGAAGAGCTACTGTCGTACTACATATCCTTCTTAAGAGCAATAAGTGGGAAGCTAGACAGGAATACAATTTCTCTGCTTGTAAAGACTCAAAATGATGAAGTGGTTTCATTTCCACTCTATGTTGAGGCAATACGATTTGCCTTTCATGAGGAGAGCATGGTCCGCACTGCCATACGGGCTTTGACACTTAATGTTTATCATGTGGGAGATGAAAGTGTCAACAGATATATCACCAGTGCCCCTCATGCAGATTATTTTTCAAACCTAGTTACATTTTTCCGAAAGCAGTGCATCAATCTAAATGCCTTGGTTTCAGACTCTATGAAAAATCCGGGTGCAGAGTCTGCAGACACAACTGCATTAATTTCTGCTGCTGTTGATGAGATCGAGGACAATCTCTACTATTTTAGTGATGTTATTTCTGCAGGAATTCCTGATGTTGGGAGGTTAATTACAGATAACATCTTGAAGCTTTTGATATTCCCGCTGCTTCTTCCTTCACTTACAATAAAAGCTGTCGAAGGGATACAAATTGGTGCTGCCACGTCTTTGTACTTGGTTTGTTGCATCCTGCACATAGTGAAAATCAAGGATTTGGCAAATACCATTTGTGCTGCTCTTTTCTTTCCATTGGATTCTTTCTTTCCAAAGTCTGGAGCTAAACCAAATGGCTGTATGCCTTTCTATGGCTTTGCACATGAAAGCCAACCGCCAGGCAGTAATACCAAGGAAGGTGTTGGAATATTAAGAGTTGACGTGACAAACTTGTCAAGCTCTCCCCAAATCCACTCAGTGGATGTCACAAGAGAAAATGATTATTGTGATTCTCATTTGTCTTTGAGGGAGGCTTTGATTTCTTATATTGCGAGTGGGGATGATATACAAGTTGCTGGTTCGTTGACTGTACTAGCTACTTTACTGCAGACAAAAGAATTGGATGAATTAATGCTAGATGCTCTTGGAATTCTTCCTCAACGCAAACAACATAAGAAACTTCTGCTGCAAGCTTTGGTTGGTGAAGTCTCAGGAGAAGAGCAACTTTTTTCTTCAGAAGGTAGCTCGTCAAAAGATGGTATTGAATGTGGTAGTGAGCTTGATAGTTCTCTACAAAAGCTTAAGGAACAGTTTGGACTGTCATGTTCTTCTCTGGAAGTGAGGGCTAGTCCTCAAGTGCATAGATTTCAGGTGCTTGATGCATTGGTCAGTCTTTTTTGCCGTTCAAATATATCTGCAGAGACATTATGGTATGGTGGTTGGCTTTTGCGCCAGTTACTTCCTTATAGCGAGGCGGAGTTTAACAGCCATCATCTCAAGATGCTGAATGATTCATATAAGAACTGTGCTAGTGCTCTTCTAATGGAGACTAGAGGAGTCTGGCCAGATCTTCTTGTAACAGTCCTCTGCGATGAATGGAAACGGTGCAAGAGAGCAATAGAGGCCTTTGCCCCTCGGAAAGAGCCCAAGTGTATTCTGTTGTCATCTCAGAGGTTCTCGTCTGAAGATGGTATCACAAGTGATTCATCATTTGCTGCTGGTGAAAGGATGTGTGAGTTGGTCAAGGTGTTTGTACTCCTACATCAACTTCAAATTTTCTCACTTGGTAGAACTTTGCCTGAGAAACCTCCTATTAAACCTCCAGCTGATGCTTTTGAATATTCCCGTGCACAAAGTGCTGGCCTAGATGCTGCAGGTCCAAAGCTTGGCACTGAGCTAAGACTTGTTGATGCTGTACCTTGTAGAATTGCGTTTGAGAGGGGTAAAGAACGTCATTTTTCCTTCCTAGCAATCTCTTTGGGTGCATCTGGCTGGGTTGTTTTAGCTGAAGAGTTACCCTTAAAGGAGCCTTATGGAGTTGTTCGTATGGTTGCTCCTTTGGCCGGTACCAATCCAAAACCTGATGATAGGCATTCAAGATGGTTACACTTGCGAATCCGTCCCTCCACTTTACCGTTTGTGGAGCCTGCTAGATATGGTGCCTACGGGAAAGCAAAGACAAAAGCTTTGGTTGATGGAAGATGGACCCTAGCATTCAGGGATGAAGAGTCTTGCAACTCTGCTTTGTCTATGGTTTTGGAGGAGATGAACCTACAGAGCAATGAGGTAGAGAGAAGATTAAAGCCCTTACTCGACCTCGAGAACTTTGTAGAATCTTCAAACTCTTCTCGAGATCCTCCTCGAACTTCCTCTCCTCATTCAACACTTTCCAATTCTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0007034 GO:0008150 GO:0008152 GO:0009058 GO:0009718 GO:0009812 GO:0009813 GO:0009987 GO:0010638 GO:0012505 GO:0016020 GO:0016192 GO:0019898 GO:0030154 GO:0032502 GO:0033043 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044088 GO:0044090 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045165 GO:0046148 GO:0046283 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0065007 GO:0071702 GO:0071704 GO:1901576 GO:1903415

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FPL

Protein Analysis

883

Amino Acids

99.1

Weight (kDa)

5.77

Isoelectric Point (pI)

42.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FPL	PF09758	60 - 210	2.6e-54	Uncharacterised conserved protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006121)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28430
fragaria_vesca	FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030
malus_domestica	MD13G1240300.v1.1
prunus_persica	Prupe.1G073600_v2.0.a1 Prupe.1G073600_v2.0.a1 Prupe.1G073600_v2.0.a1
pyrus_communis	pycom13g21280 pycom16g20730
rosa_chinensis	RchiOBHm_Chr4g0399151
rosa_laevigata	RLG00000009295
rosa_multiflora	Rmu_sc0005882.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344120
rosa_rugosa	Rorug04G0014200
rosa_samantha	Rh4AG092100 Rh4BG090200 Rh4CG100600 Rh4DG084700
rosa_wichuraiana	Rw4G007610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1198
Acc36I	ACCTGC	1 cut(s)	2111
Acc65I	GGTACC	1 cut(s)	2312
AccB1I	GGYRCC	2 cut(s)	2312, 2405
AccB7I	CCANNNNNTGG	3 cut(s)	1085, 1225, 2132
AciI	CCGC	5 cut(s)	191, 595, 917, 1142, 1721
AclWI	GGATC	4 cut(s)	36, 177, 334, 2594
AcoI	YGGCCR	2 cut(s)	1808, 2307
AcsI	RAATTY	4 cut(s)	236, 861, 1395, 2019
AcuI	CTGAAG	4 cut(s)	296, 1464, 1950, 2274
AdeI	CACNNNGTG	3 cut(s)	1012, 1225, 2102
AfaI	GTAC	7 cut(s)	273, 449, 986, 1338, 2001, 2163, 2314
AfiI	CCNNNNNNNGG	6 cut(s)	872, 1085, 1118, 1225, 2132, 2309
AflII	CTTAAG	2 cut(s)	463, 1547
AflIII	ACRYGT	1 cut(s)	375
AjiI	CACGTC	2 cut(s)	978, 1189
AjnI	CCWGG	2 cut(s)	23, 1144
AloI	GAACNNNNNNTCC	2 cut(s)	2602, 2634
Alw21I	GWGCWC	3 cut(s)	51, 1783, 2098
Alw26I	GTCTC	4 cut(s)	204, 1459, 1661, 1787
Alw44I	GTGCAC	1 cut(s)	2094
AlwI	GGATC	4 cut(s)	36, 177, 334, 2594
AlwNI	CAGNNNCTG	1 cut(s)	776
Ama87I	CYCGRG	2 cut(s)	2564, 2595
AoxI	GGCC	5 cut(s)	97, 1808, 1869, 2107, 2307
ApaLI	GTGCAC	1 cut(s)	2094
ApeKI	GCWGC	7 cut(s)	803, 919, 971, 1046, 1432, 1961, 2117
ApoI	RAATTY	4 cut(s)	236, 861, 1395, 2019
ArsI	GACNNNNNNTTYG	2 cut(s)	2416, 2448
AseI	ATTAAT	2 cut(s)	794, 1376
Asp718I	GGTACC	1 cut(s)	2312
AspLEI	GCGC	3 cut(s)	95, 178, 1698
AspS9I	GGNCC	4 cut(s)	98, 592, 2123, 2451
AsuC2I	CCSGG	1 cut(s)	765
AsuHPI	GGTGA	3 cut(s)	655, 1460, 1979
AvaI	CYCGRG	2 cut(s)	2564, 2595
AvaII	GGWCC	3 cut(s)	592, 2123, 2451
BaeGI	GKGCMC	2 cut(s)	673, 2098
BalI	TGGCCA	1 cut(s)	1810
BanI	GGYRCC	2 cut(s)	2312, 2405
BanII	GRGCYC	2 cut(s)	51, 1893
Bbv12I	GWGCWC	3 cut(s)	51, 1783, 2098
BbvI	GCAGC	7 cut(s)	790, 906, 958, 1033, 1419, 1948, 2104
BccI	CCATC	6 cut(s)	1496, 1743, 1928, 2340, 2435, 2442
BceAI	ACGGC	1 cut(s)	1632
BcgI	CGANNNNNNTGC	2 cut(s)	702, 736
BciT130I	CCWGG	2 cut(s)	25, 1146
BciVI	GTATCC	1 cut(s)	951
BclI	TGATCA	1 cut(s)	118
BcnI	CCSGG	1 cut(s)	765
BcoDI	GTCTC	4 cut(s)	204, 1459, 1661, 1787
BfmI	CTRYAG	7 cut(s)	320, 777, 855, 1352, 1662, 2118, 2519
BfoI	RGCGCY	1 cut(s)	179
BfrI	CTTAAG	2 cut(s)	463, 1547
BfuAI	ACCTGC	1 cut(s)	2111
BfuI	GTATCC	1 cut(s)	951
BglI	GCCNNNNNGGC	1 cut(s)	608
BglII	AGATCT	1 cut(s)	1813
BisI	GCNGC	7 cut(s)	804, 920, 972, 1047, 1433, 1962, 2118
BlsI	GCNGC	7 cut(s)	805, 921, 973, 1048, 1434, 1963, 2119
Bme1390I	CCNGG	3 cut(s)	25, 765, 1146
Bme18I	GGWCC	3 cut(s)	592, 2123, 2451
BmeT110I	CYCGRG	2 cut(s)	2564, 2595
BmgBI	CACGTC	2 cut(s)	978, 1189
BmgT120I	GGNCC	4 cut(s)	98, 592, 2123, 2451
BmiI	GGNNCC	6 cut(s)	100, 2274, 2314, 2391, 2407, 2453
BmrFI	CCNGG	3 cut(s)	25, 765, 1146
BmsI	GCATC	9 cut(s)	732, 1008, 1375, 1615, 1737, 2065, 2104, 2146, 2240
BpmI	CTGGAG	2 cut(s)	1107, 2052
Bpu10I	CCTNAGC	1 cut(s)	50
BpuEI	CTTGAG	2 cut(s)	1584, 1727
BpuMI	CCSGG	1 cut(s)	765
BsaAI	YACGTR	1 cut(s)	275
BsaI	GGTCTC	1 cut(s)	204
BsaJI	CCNNGG	4 cut(s)	24, 738, 1158, 1880
BsaXI	ACNNNNNCTCC	4 cut(s)	255, 285, 2602, 2632
Bsc4I	CCNNNNNNNGG	6 cut(s)	872, 1085, 1118, 1225, 2132, 2309
Bse118I	RCCGGY	1 cut(s)	2309
Bse1I	ACTGG	3 cut(s)	74, 666, 1699
Bse3DI	GCAATG	2 cut(s)	186, 2533
BseBI	CCWGG	2 cut(s)	25, 1146
BseDI	CCNNGG	4 cut(s)	24, 738, 1158, 1880
BseGI	GGATG	7 cut(s)	60, 999, 1234, 1312, 1375, 1980, 2473
BseLI	CCNNNNNNNGG	6 cut(s)	872, 1085, 1118, 1225, 2132, 2309
BseMI	GCAATG	2 cut(s)	186, 2533
BseMII	CTCAG	7 cut(s)	64, 170, 1236, 1470, 1928, 2037, 2130
BseNI	ACTGG	3 cut(s)	74, 666, 1699
BseRI	GAGGAG	6 cut(s)	100, 596, 1815, 2522, 2595, 2610
BseSI	GKGCMC	2 cut(s)	673, 2098
BseXI	GCAGC	7 cut(s)	790, 906, 958, 1033, 1419, 1948, 2104
BseYI	CCCAGC	1 cut(s)	2238
BsgI	GTGCAG	2 cut(s)	789, 989
BshFI	GGCC	5 cut(s)	99, 1810, 1871, 2109, 2309
BshNI	GGYRCC	2 cut(s)	2312, 2405
BsiHKAI	GWGCWC	3 cut(s)	51, 1783, 2098
BsiHKCI	CYCGRG	2 cut(s)	2564, 2595
BsiSI	CCGG	2 cut(s)	764, 2310
BslFI	GGGAC	1 cut(s)	2352
BslI	CCNNNNNNNGG	6 cut(s)	872, 1085, 1118, 1225, 2132, 2309
BsmAI	GTCTC	4 cut(s)	204, 1459, 1661, 1787
BsmFI	GGGAC	1 cut(s)	2352
BsmI	GAATGC	2 cut(s)	2338, 2459
BsnI	GGCC	5 cut(s)	99, 1810, 1871, 2109, 2309
Bso31I	GGTCTC	1 cut(s)	204
BsoBI	CYCGRG	2 cut(s)	2564, 2595
Bsp1286I	GDGCHC	5 cut(s)	51, 673, 1783, 1893, 2098
Bsp143I	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
BspACI	CCGC	5 cut(s)	191, 595, 917, 1142, 1721
BspANI	GGCC	5 cut(s)	99, 1810, 1871, 2109, 2309
BspCNI	CTCAG	7 cut(s)	63, 171, 1235, 1469, 1927, 2038, 2131
BspHI	TCATGA	1 cut(s)	577
BspLI	GGNNCC	6 cut(s)	100, 2274, 2314, 2391, 2407, 2453
BspMAI	CTGCAG	5 cut(s)	781, 859, 1356, 1666, 2122
BspMI	ACCTGC	1 cut(s)	2111
BspPI	GGATC	4 cut(s)	36, 177, 334, 2594
BspQI	GCTCTTC	3 cut(s)	429, 1458, 1788
BspT107I	GGYRCC	2 cut(s)	2312, 2405
BspTI	CTTAAG	2 cut(s)	463, 1547
BspTNI	GGTCTC	1 cut(s)	204
BsrDI	GCAATG	2 cut(s)	186, 2533
BsrFI	RCCGGY	1 cut(s)	2309
BsrI	ACTGG	3 cut(s)	74, 666, 1699
BssAI	RCCGGY	1 cut(s)	2309
BssECI	CCNNGG	4 cut(s)	24, 738, 1158, 1880
BssMI	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
BssT1I	CCWWGG	2 cut(s)	738, 1158
Bst2UI	CCWGG	2 cut(s)	25, 1146
Bst4CI	ACNGT	9 cut(s)	304, 444, 1336, 1557, 1566, 1772, 1828, 1851, 2382
Bst6I	CTCTTC	6 cut(s)	429, 1458, 1788, 2250, 2466, 2596
BstAFI	CTTAAG	2 cut(s)	463, 1547
BstAPI	GCANNNNNTGC	2 cut(s)	776, 2102
BstBAI	YACGTR	1 cut(s)	275
BstC8I	GCNNGC	4 cut(s)	97, 1437, 2107, 2394
BstDEI	CTNAG	9 cut(s)	40, 50, 179, 1222, 1456, 1914, 2046, 2139, 2144
BstF5I	GGATG	7 cut(s)	60, 999, 1234, 1312, 1375, 1980, 2473
BstH2I	RGCGCY	1 cut(s)	179
BstHHI	GCGC	3 cut(s)	95, 178, 1698
BstKTI	GATC	7 cut(s)	31, 121, 185, 342, 819, 1816, 2602
BstMAI	GTCTC	4 cut(s)	204, 1459, 1661, 1787
BstMBI	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
BstMWI	GCNNNNNNNGC	7 cut(s)	594, 608, 677, 776, 1868, 2102, 2306
BstNI	CCWGG	2 cut(s)	25, 1146
BstNSI	RCATGY	1 cut(s)	379
BstSCI	CCNGG	3 cut(s)	23, 763, 1144
BstSFI	CTRYAG	7 cut(s)	320, 777, 855, 1352, 1662, 2118, 2519
BstSLI	GKGCMC	2 cut(s)	673, 2098
BstSNI	TACGTA	1 cut(s)	275
BstV1I	GCAGC	7 cut(s)	790, 906, 958, 1033, 1419, 1948, 2104
BstX2I	RGATCY	3 cut(s)	182, 1813, 2599
BstYI	RGATCY	3 cut(s)	182, 1813, 2599
BsuI	GTATCC	1 cut(s)	951
BsuRI	GGCC	5 cut(s)	99, 1810, 1871, 2109, 2309
BtgZI	GCGATG	1 cut(s)	1851
BtrI	CACGTC	2 cut(s)	978, 1189
BtsCI	GGATG	7 cut(s)	60, 999, 1234, 1312, 1375, 1980, 2473
BtsI	GCAGTG	2 cut(s)	597, 725
BtsIMutI	CAGTG	6 cut(s)	67, 597, 673, 725, 1230, 2136
BveI	ACCTGC	1 cut(s)	2111
Cac8I	GCNNGC	4 cut(s)	97, 1437, 2107, 2394
CaiI	CAGNNNCTG	1 cut(s)	776
CciI	TCATGA	1 cut(s)	577
CfoI	GCGC	3 cut(s)	95, 178, 1698
Cfr10I	RCCGGY	1 cut(s)	2309
Cfr13I	GGNCC	4 cut(s)	98, 592, 2123, 2451
Csp6I	GTAC	7 cut(s)	272, 448, 985, 1337, 2000, 2162, 2313
CspCI	CAANNNNNGTGG	2 cut(s)	214, 249
CviQI	GTAC	7 cut(s)	272, 448, 985, 1337, 2000, 2162, 2313
DdeI	CTNAG	9 cut(s)	40, 50, 179, 1222, 1456, 1914, 2046, 2139, 2144
DpnI	GATC	7 cut(s)	30, 120, 184, 341, 818, 1815, 2601
DpnII	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
DraIII	CACNNNGTG	3 cut(s)	1012, 1225, 2102
DrdI	GACNNNNNNGTC	1 cut(s)	1198
DseDI	GACNNNNNNGTC	1 cut(s)	1198
EaeI	YGGCCR	2 cut(s)	1808, 2307
Eam1104I	CTCTTC	6 cut(s)	429, 1458, 1788, 2250, 2466, 2596
EarI	CTCTTC	6 cut(s)	429, 1458, 1788, 2250, 2466, 2596
EciI	GGCGGA	1 cut(s)	1736
Ecl136II	GAGCTC	1 cut(s)	49
Eco105I	TACGTA	1 cut(s)	275
Eco130I	CCWWGG	2 cut(s)	738, 1158
Eco147I	AGGCCT	1 cut(s)	1871
Eco24I	GRGCYC	2 cut(s)	51, 1893
Eco31I	GGTCTC	1 cut(s)	204
Eco47I	GGWCC	3 cut(s)	592, 2123, 2451
Eco53kI	GAGCTC	1 cut(s)	49
Eco57I	CTGAAG	4 cut(s)	296, 1464, 1950, 2274
Eco88I	CYCGRG	2 cut(s)	2564, 2595
EcoICRI	GAGCTC	1 cut(s)	49
EcoO109I	RGGNCCY	1 cut(s)	98
EcoRI	GAATTC	2 cut(s)	861, 1395
EcoRII	CCWGG	2 cut(s)	23, 1144
EcoT14I	CCWWGG	2 cut(s)	738, 1158
EcoT22I	ATGCAT	1 cut(s)	1630
EcoT38I	GRGCYC	2 cut(s)	51, 1893
ErhI	CCWWGG	2 cut(s)	738, 1158
FalI	AAGNNNNNCTT	2 cut(s)	1455, 1487
FaqI	GGGAC	1 cut(s)	2352
FauI	CCCGC	1 cut(s)	924
FauNDI	CATATG	1 cut(s)	393
FbaI	TGATCA	1 cut(s)	118
Fnu4HI	GCNGC	7 cut(s)	804, 920, 972, 1047, 1433, 1962, 2118
FokI	GGATG	7 cut(s)	67, 986, 1241, 1319, 1382, 1987, 2480
FriOI	GRGCYC	2 cut(s)	51, 1893
Fsp4HI	GCNGC	7 cut(s)	804, 920, 972, 1047, 1433, 1962, 2118
GlaI	GCGC	3 cut(s)	94, 177, 1697
GluI	GCNGC	7 cut(s)	804, 920, 972, 1047, 1433, 1962, 2118
GsaI	CCCAGC	1 cut(s)	2242
GsuI	CTGGAG	2 cut(s)	1107, 2052
HaeII	RGCGCY	1 cut(s)	179
HaeIII	GGCC	5 cut(s)	99, 1810, 1871, 2109, 2309
HapII	CCGG	2 cut(s)	764, 2310
HhaI	GCGC	3 cut(s)	95, 178, 1698
Hin6I	GCGC	3 cut(s)	93, 176, 1696
HinP1I	GCGC	3 cut(s)	93, 176, 1696
HincII	GTYRAC	3 cut(s)	652, 1186, 1332
HindII	GTYRAC	3 cut(s)	652, 1186, 1332
HindIII	AAGCTT	5 cut(s)	902, 1437, 1544, 2129, 2430
HpaII	CCGG	2 cut(s)	764, 2310
HphI	GGTGA	3 cut(s)	655, 1460, 1979
Hpy166II	GTNNAC	5 cut(s)	154, 652, 1186, 1332, 2096
Hpy188I	TCNGA	6 cut(s)	713, 748, 1483, 1884, 1917, 1930
Hpy8I	GTNNAC	5 cut(s)	154, 652, 1186, 1332, 2096
HpyAV	CCTTC	7 cut(s)	277, 469, 939, 947, 1157, 1478, 2218
HpyCH4III	ACNGT	9 cut(s)	304, 444, 1336, 1557, 1566, 1772, 1828, 1851, 2382
HpyCH4IV	ACGT	6 cut(s)	203, 274, 410, 977, 1188, 2197
HpyF10VI	GCNNNNNNNGC	7 cut(s)	594, 608, 677, 776, 1868, 2102, 2306
HpyF3I	CTNAG	9 cut(s)	40, 50, 179, 1222, 1456, 1914, 2046, 2139, 2144
HpySE526I	ACGT	6 cut(s)	203, 274, 410, 977, 1188, 2197
HspAI	GCGC	3 cut(s)	93, 176, 1696
KpnI	GGTACC	1 cut(s)	2316
Ksp22I	TGATCA	1 cut(s)	118
Kzo9I	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
LguI	GCTCTTC	3 cut(s)	429, 1458, 1788
LmnI	GCTCC	4 cut(s)	1088, 2272, 2305, 2389
Lsp1109I	GCAGC	7 cut(s)	790, 906, 958, 1033, 1419, 1948, 2104
LweI	GCATC	9 cut(s)	732, 1008, 1375, 1615, 1737, 2065, 2104, 2146, 2240
MaeII	ACGT	6 cut(s)	203, 274, 410, 977, 1188, 2197
MaeIII	GTNAC	9 cut(s)	306, 315, 700, 1189, 1231, 1701, 1822, 2259, 2349
MalI	GATC	7 cut(s)	30, 120, 184, 341, 818, 1815, 2601
MboI	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
MflI	RGATCY	3 cut(s)	182, 1813, 2599
MhlI	GDGCHC	5 cut(s)	51, 673, 1783, 1893, 2098
MlsI	TGGCCA	1 cut(s)	1810
MluNI	TGGCCA	1 cut(s)	1810
MlyI	GAGTC	6 cut(s)	215, 511, 743, 782, 1812, 2483
MmeI	TCCRAC	1 cut(s)	1150
Mox20I	TGGCCA	1 cut(s)	1810
Mph1103I	ATGCAT	1 cut(s)	1630
MscI	TGGCCA	1 cut(s)	1810
Msp20I	TGGCCA	1 cut(s)	1810
MspA1I	CMGCKG	3 cut(s)	124, 919, 2072
MspCI	CTTAAG	2 cut(s)	463, 1547
MspI	CCGG	2 cut(s)	764, 2310
MspR9I	CCNGG	3 cut(s)	25, 765, 1146
Mva1269I	GAATGC	2 cut(s)	2338, 2459
MvaI	CCWGG	2 cut(s)	25, 1146
MwoI	GCNNNNNNNGC	7 cut(s)	594, 608, 677, 776, 1868, 2102, 2306
NciI	CCSGG	1 cut(s)	765
NdeI	CATATG	1 cut(s)	393
NdeII	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
NlaIV	GGNNCC	6 cut(s)	100, 2274, 2314, 2391, 2407, 2453
NmuCI	GTSAC	3 cut(s)	306, 1189, 1231
NsiI	ATGCAT	1 cut(s)	1630
NspI	RCATGY	1 cut(s)	379
PaeR7I	CTCGAG	2 cut(s)	2564, 2595
PagI	TCATGA	1 cut(s)	577
PceI	AGGCCT	1 cut(s)	1871
PciI	ACATGT	1 cut(s)	375
PciSI	GCTCTTC	3 cut(s)	429, 1458, 1788
PctI	GAATGC	2 cut(s)	2338, 2459
PfeI	GAWTC	6 cut(s)	1067, 1256, 1757, 1949, 2361, 2579
PflFI	GACNNNGTC	1 cut(s)	304
PflMI	CCANNNNNTGG	3 cut(s)	1085, 1225, 2132
PkrI	GCNGC	7 cut(s)	805, 921, 973, 1048, 1434, 1963, 2119
PleI	GAGTC	6 cut(s)	215, 511, 743, 781, 1811, 2482
PpsI	GAGTC	6 cut(s)	215, 511, 743, 781, 1811, 2482
Ppu21I	YACGTR	1 cut(s)	275
PscI	ACATGT	1 cut(s)	375
PshBI	ATTAAT	2 cut(s)	794, 1376
Psp124BI	GAGCTC	1 cut(s)	51
Psp6I	CCWGG	2 cut(s)	23, 1144
PspFI	CCCAGC	1 cut(s)	2238
PspGI	CCWGG	2 cut(s)	23, 1144
PspN4I	GGNNCC	6 cut(s)	100, 2274, 2314, 2391, 2407, 2453
PspPI	GGNCC	4 cut(s)	98, 592, 2123, 2451
PstI	CTGCAG	5 cut(s)	781, 859, 1356, 1666, 2122
PstNI	CAGNNNCTG	1 cut(s)	776
PsuI	RGATCY	3 cut(s)	182, 1813, 2599
PsyI	GACNNNGTC	1 cut(s)	304
PvuII	CAGCTG	2 cut(s)	124, 2072
RsaI	GTAC	7 cut(s)	273, 449, 986, 1338, 2001, 2163, 2314
RsaNI	GTAC	7 cut(s)	272, 448, 985, 1337, 2000, 2162, 2313
SacI	GAGCTC	1 cut(s)	51
SapI	GCTCTTC	3 cut(s)	429, 1458, 1788
SatI	GCNGC	7 cut(s)	804, 920, 972, 1047, 1433, 1962, 2118
Sau3AI	GATC	7 cut(s)	28, 118, 182, 339, 816, 1813, 2599
Sau96I	GGNCC	4 cut(s)	98, 592, 2123, 2451
SchI	GAGTC	6 cut(s)	215, 511, 743, 782, 1812, 2483
ScrFI	CCNGG	3 cut(s)	25, 765, 1146
SduI	GDGCHC	5 cut(s)	51, 673, 1783, 1893, 2098
SfaNI	GCATC	9 cut(s)	732, 1008, 1375, 1615, 1737, 2065, 2104, 2146, 2240
SfcI	CTRYAG	7 cut(s)	320, 777, 855, 1352, 1662, 2118, 2519
Sfr274I	CTCGAG	2 cut(s)	2564, 2595
SinI	GGWCC	3 cut(s)	592, 2123, 2451
SlaI	CTCGAG	2 cut(s)	2564, 2595
SmlI	CTYRAG	6 cut(s)	463, 1547, 1599, 1742, 2564, 2595
SmoI	CTYRAG	6 cut(s)	463, 1547, 1599, 1742, 2564, 2595
SnaBI	TACGTA	1 cut(s)	275
SseBI	AGGCCT	1 cut(s)	1871
SsiI	CCGC	5 cut(s)	191, 595, 917, 1142, 1721
SspI	AATATT	2 cut(s)	1176, 2087
SstI	GAGCTC	1 cut(s)	51
StuI	AGGCCT	1 cut(s)	1871
StyD4I	CCNGG	3 cut(s)	23, 763, 1144
StyI	CCWWGG	2 cut(s)	738, 1158
TaaI	ACNGT	9 cut(s)	304, 444, 1336, 1557, 1566, 1772, 1828, 1851, 2382
TaiI	ACGT	6 cut(s)	206, 277, 413, 980, 1191, 2200
TaqI	TCGA	6 cut(s)	819, 951, 2559, 2565, 2596, 2608
TatI	WGTACW	3 cut(s)	984, 1336, 1999
TfiI	GAWTC	6 cut(s)	1067, 1256, 1757, 1949, 2361, 2579
TscAI	CASTG	6 cut(s)	74, 604, 673, 725, 1230, 2143
TseFI	GTSAC	3 cut(s)	306, 1189, 1231
TseI	GCWGC	7 cut(s)	803, 919, 971, 1046, 1432, 1961, 2117
Tsp45I	GTSAC	3 cut(s)	306, 1189, 1231
TspGWI	ACGGA	1 cut(s)	2354
TspRI	CASTG	6 cut(s)	74, 604, 673, 725, 1230, 2143
Tth111I	GACNNNGTC	1 cut(s)	304
Van91I	CCANNNNNTGG	3 cut(s)	1085, 1225, 2132
Vha464I	CTTAAG	2 cut(s)	463, 1547
VneI	GTGCAC	1 cut(s)	2094
VpaK11BI	GGWCC	3 cut(s)	592, 2123, 2451
VspI	ATTAAT	2 cut(s)	794, 1376
XapI	RAATTY	4 cut(s)	236, 861, 1395, 2019
XceI	RCATGY	1 cut(s)	379
XhoI	CTCGAG	2 cut(s)	2564, 2595
Zsp2I	ATGCAT	1 cut(s)	1630

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.