Rh4CG100600

Uncharacterised conserved protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006121
Physical Location & Seq
Forward (+)
18524628 .. 18536767
12140 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG100600.1

Sequence Viewer

Length: 1962 bp
ATGTGGTTTTCGTTTTGGAGATCCAGGGATCGATTCTCCTTCGATGAGCTCAGGTATTTAACTGATCAGCTGGTGAAAATTCAAGTTGTGAATGATGTTAACAAGGATTTCGTTATTGAAGCTCTGAGATCAATTGCGGAGTTGATAACTTATGGCGACCAACATGACACAACTTTTTTTGAATTTTTTATGGAGAAGCAAATCATGGGAGAGTTTGTACGTATATTGAAGATTAGCAGAAATATGACTGTTTCACTTCAGTTGCTGCAGACAATCAGCATTATGATACAGAACCTAAAAAATGAACATGCTATATATTACATGTTCAGTAATGAACATATGAACTACTTAATAACGTACTCATTTGATTTTAAGAATGAAGAGCTACTTTCTTACTATATATCCTTCTTAAGAGCAATAAGTGGAAAGCTAGATAGAAATACAATTTCTCTGCTTGTAAAGACTCAAGAGGATGAAGTTGTTTCATTTCCGCTGTATGTTGAGGCAATACGATTTGCTTTTCATGAGGAGAGCATGGTCCGCACTGCTGTACGGGCTCTTACGCTTAATGTTTATCACGTTGGAGATGATTCTGTCAATAGATATGTCACCAATGCCCCTCATTCAGATTATTTTTCAAACTTGGTTAAATTTTTCCGGAAGCAGTGCATCGATCTAAATGTATTGGTTTCTGGCACTACGACAAACCTGGGTGCAGACACAACCTTGTCAATTCCTGCTGCTGTTGATGAGATCGAGGACAATCTCTACTATTTCAGTGATATTATTTCTGCCGGAATTCCTGATGTTGGGAGGTTATTCACAGACAACATATTGCAGCTTTTGATATTTCCATTGCTTCTACCTTCTCTAACACTAAAAGCTGTCAAGGGGATTCAAATGGGTGCTGTCACGTCTTTGTATTTGATTTGTTGCATCTTGCGCATAGTAAAAATCAAGGATCTGTCAAATACTATTGCTGCTGCCCTATTCTGTCCGTTAGAAGCTTTCTTATCAAAGTCTGTTGCTGAACCCAATGGCTACATGTCTGGTTATGATGTTGCCCATGAAAGCCAACCACCAGGCAGTAATAATCTAATTAAGGCAGAAGTGGGAAATTTAAGTGTTGATGTGGCAAACTTGTCAATCTCTCCCCAAATCCAGCCAGTTGATGTCAGTATTGCAACAGGAAATGATAGTTGTGATTGTCATTTGCCTTTGAGGGAGGCTTTGCTTTCTTACCTTACGAGTGGGAACGATGTACAAGTTTCGGGTTCATTGAGTGTGCTGGCTACTTTGTTGCAAACAAAAGAATTGGATGAATCAATGTCAGATGCTCTGGGAATACTTCCTCAACGCAAACAGCATAAGAAACTTCTGCTGCAAGCTTTGGTTGGTGAAAGCTCAGGTGAAGAGCAACTTTTTTCTTCAGAAAGTAGCTCCTTGAGAAACGGAATTGAATTTGGCAGTGAGCTTGATGGCTGTCTACAAAAGCTTAAGGAACAGTATGGAGTATCATGTTCTTTTCTGGAAGTGAGAGCTAGCCCTCGTGTACATAGATTTCAGGTGCTTGATGCATTGGTCAGTGTCTTCTGCCGTTCAAATTTATCGGCGGAGACTTTATGGGATGGAGGTTGGCTTTTGCGTCAATTGCTTCCTTATAGTGAGGCAGAATTCAACAGCCATCATCGGAAGTTGCTGAAGGAATCGTACAAGAACTGTGCTAGTGCTCTTATTGAGGAGACTAGAGGAATATGGCCGGATATACTTGTAACAGTGCTCTGTGATGAATGGAAAAAGTGCAAGAGAGGAATAGAGGCATCATCCCCTCGCAAAGAGCCCAAGGACATTTTGTTTTCATCTCGAAAGTTCTCCACTGAAGATGGTATCACAAGCGATTCATCGTTTGCTGCTGGTGAAAGGATGTGCGAGTTGGTGAAGGTTTGTGTCTGTACTAATTGA

Protein Analysis

653

Amino Acids

73.49

Weight (kDa)

5.22

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FPL PF09758 42 - 192 4.8e-55 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 944
AccI GTMKAC 1 cut(s) 1486
AccIII TCCGGA 1 cut(s) 657
AciI CCGC 4 cut(s) 137, 491, 541, 1613
AclWI GGATC 3 cut(s) 15, 36, 969
AcoI YGGCCR 1 cut(s) 1757
AcsI RAATTY 8 cut(s) 78, 182, 650, 798, 1117, 1460, 1603, 1673
AcuI CTGAAG 4 cut(s) 242, 1413, 1721, 1899
AfaI GTAC 7 cut(s) 219, 359, 552, 1263, 1554, 1712, 1954
AfiI CCNNNNNNNGG 1 cut(s) 809
AflII CTTAAG 2 cut(s) 409, 1496
AflIII ACRYGT 2 cut(s) 321, 1044
AgsI TTSAA 9 cut(s) 83, 119, 182, 229, 639, 899, 1460, 1602, 1678
AjiI CACGTC 1 cut(s) 915
AjnI CCWGG 3 cut(s) 23, 708, 1081
Alw21I GWGCWC 3 cut(s) 51, 1732, 1782
Alw26I GTCTC 2 cut(s) 1610, 1736
AlwI GGATC 3 cut(s) 15, 36, 969
AlwNI CAGNNNCTG 1 cut(s) 265
Aor13HI TCCGGA 1 cut(s) 657
AoxI GGCC 1 cut(s) 1757
ApeKI GCWGC 7 cut(s) 265, 740, 838, 980, 983, 1381, 1910
ApoI RAATTY 8 cut(s) 78, 182, 650, 798, 1117, 1460, 1603, 1673
AspLEI GCGC 1 cut(s) 945
AspS9I GGNCC 1 cut(s) 538
AsuHPI GGTGA 6 cut(s) 85, 601, 1409, 1421, 1928, 1948
AsuNHI GCTAGC 1 cut(s) 1541
AvaII GGWCC 1 cut(s) 538
BanII GRGCYC 3 cut(s) 51, 559, 1842
BarI GAAGNNNNNNTAC 2 cut(s) 1694, 1726
BauI CACGAG 1 cut(s) 1548
BbsI GAAGAC 1 cut(s) 1582
Bbv12I GWGCWC 3 cut(s) 51, 1732, 1782
BbvI GCAGC 7 cut(s) 252, 727, 850, 967, 970, 1368, 1897
BccI CCATC 4 cut(s) 1472, 1622, 1692, 1877
BceAI ACGGC 1 cut(s) 1581
BciT130I CCWGG 3 cut(s) 25, 710, 1083
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 1610, 1736
BfaI CTAG 4 cut(s) 431, 1542, 1725, 1746
BfmI CTRYAG 1 cut(s) 266
BfrI CTTAAG 2 cut(s) 409, 1496
BisI GCNGC 7 cut(s) 266, 741, 839, 981, 984, 1382, 1911
BlsI GCNGC 7 cut(s) 267, 742, 840, 982, 985, 1383, 1912
Bme1390I CCNGG 3 cut(s) 25, 710, 1083
Bme18I GGWCC 1 cut(s) 538
BmgBI CACGTC 1 cut(s) 915
BmgT120I GGNCC 1 cut(s) 538
BmrFI CCNGG 3 cut(s) 25, 710, 1083
BmsI GCATC 5 cut(s) 678, 945, 1324, 1564, 1829
BmtI GCTAGC 1 cut(s) 1545
BpiI GAAGAC 1 cut(s) 1582
Bpu10I CCTNAGC 2 cut(s) 50, 1405
BpuEI CTTGAG 2 cut(s) 450, 1465
Bsa29I ATCGAT 2 cut(s) 31, 672
BsaAI YACGTR 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 24, 709, 1842
BsaWI WCCGGW 1 cut(s) 657
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 809
Bse1I ACTGG 1 cut(s) 1166
Bse3DI GCAATG 1 cut(s) 854
BseAI TCCGGA 1 cut(s) 657
BseBI CCWGG 3 cut(s) 25, 710, 1083
BseCI ATCGAT 2 cut(s) 31, 672
BseDI CCNNGG 3 cut(s) 24, 709, 1842
BseGI GGATG 5 cut(s) 478, 1324, 1633, 1823, 1929
BseLI CCNNNNNNNGG 1 cut(s) 809
BseMI GCAATG 1 cut(s) 854
BseMII CTCAG 3 cut(s) 64, 116, 1419
BseNI ACTGG 1 cut(s) 1166
BseRI GAGGAG 2 cut(s) 542, 1754
BseXI GCAGC 7 cut(s) 252, 727, 850, 967, 970, 1368, 1897
BsgI GTGCAG 1 cut(s) 735
BshFI GGCC 1 cut(s) 1759
BshVI ATCGAT 2 cut(s) 31, 672
BsiHKAI GWGCWC 3 cut(s) 51, 1732, 1782
BsiSI CCGG 3 cut(s) 658, 795, 1760
BslI CCNNNNNNNGG 1 cut(s) 809
BsmAI GTCTC 2 cut(s) 1610, 1736
BsnI GGCC 1 cut(s) 1759
Bsp1286I GDGCHC 5 cut(s) 51, 559, 1732, 1782, 1842
Bsp13I TCCGGA 1 cut(s) 657
Bsp1407I TGTACA 2 cut(s) 1261, 1552
Bsp143I GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
BspACI CCGC 4 cut(s) 137, 491, 541, 1613
BspANI GGCC 1 cut(s) 1759
BspCNI CTCAG 3 cut(s) 63, 117, 1418
BspDI ATCGAT 2 cut(s) 31, 672
BspEI TCCGGA 1 cut(s) 657
BspHI TCATGA 1 cut(s) 523
BspMAI CTGCAG 1 cut(s) 270
BspOI GCTAGC 1 cut(s) 1545
BspPI GGATC 3 cut(s) 15, 36, 969
BspQI GCTCTTC 2 cut(s) 375, 1407
BspTI CTTAAG 2 cut(s) 409, 1496
BsrDI GCAATG 1 cut(s) 854
BsrGI TGTACA 2 cut(s) 1261, 1552
BsrI ACTGG 1 cut(s) 1166
BssECI CCNNGG 3 cut(s) 24, 709, 1842
BssMI GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
BssSI CACGAG 1 cut(s) 1548
BssT1I CCWWGG 1 cut(s) 1842
Bst2BI CACGAG 1 cut(s) 1548
Bst2UI CCWGG 3 cut(s) 25, 710, 1083
Bst4CI ACNGT 4 cut(s) 250, 1506, 1721, 1777
Bst6I CTCTTC 2 cut(s) 375, 1407
BstAFI CTTAAG 2 cut(s) 409, 1496
BstAUI TGTACA 2 cut(s) 1261, 1552
BstBAI YACGTR 1 cut(s) 221
BstC8I GCNNGC 3 cut(s) 1290, 1386, 1543
BstDEI CTNAG 3 cut(s) 50, 125, 1405
BstF5I GGATG 5 cut(s) 478, 1324, 1633, 1823, 1929
BstHHI GCGC 1 cut(s) 945
BstKTI GATC 7 cut(s) 23, 31, 67, 131, 676, 756, 964
BstMAI GTCTC 2 cut(s) 1610, 1736
BstMBI GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
BstMWI GCNNNNNNNGC 4 cut(s) 540, 554, 942, 1651
BstNI CCWGG 3 cut(s) 25, 710, 1083
BstNSI RCATGY 3 cut(s) 311, 325, 1048
BstSCI CCNGG 3 cut(s) 23, 708, 1081
BstSFI CTRYAG 1 cut(s) 266
BstSNI TACGTA 1 cut(s) 221
BstV1I GCAGC 7 cut(s) 252, 727, 850, 967, 970, 1368, 1897
BstV2I GAAGAC 1 cut(s) 1582
BstX2I RGATCY 2 cut(s) 20, 961
BstYI RGATCY 2 cut(s) 20, 961
Bsu15I ATCGAT 2 cut(s) 31, 672
BsuRI GGCC 1 cut(s) 1759
BsuTUI ATCGAT 2 cut(s) 31, 672
BtrI CACGTC 1 cut(s) 915
BtsCI GGATG 5 cut(s) 478, 1324, 1633, 1823, 1929
BtsI GCAGTG 3 cut(s) 543, 671, 1474
BtsIMutI CAGTG 7 cut(s) 543, 671, 784, 1474, 1591, 1782, 1875
Cac8I GCNNGC 3 cut(s) 1290, 1386, 1543
CaiI CAGNNNCTG 1 cut(s) 265
CciI TCATGA 1 cut(s) 523
CfoI GCGC 1 cut(s) 945
Cfr13I GGNCC 1 cut(s) 538
ClaI ATCGAT 2 cut(s) 31, 672
CseI GACGC 1 cut(s) 1634
Csp6I GTAC 7 cut(s) 218, 358, 551, 1262, 1553, 1711, 1953
CviAII CATG 9 cut(s) 164, 205, 308, 322, 524, 535, 1045, 1067, 1518
CviQI GTAC 7 cut(s) 218, 358, 551, 1262, 1553, 1711, 1953
DdeI CTNAG 3 cut(s) 50, 125, 1405
DpnI GATC 7 cut(s) 22, 30, 66, 130, 675, 755, 963
DpnII GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
EaeI YGGCCR 1 cut(s) 1757
Eam1104I CTCTTC 2 cut(s) 375, 1407
EarI CTCTTC 2 cut(s) 375, 1407
EciI GGCGGA 1 cut(s) 1628
Ecl136II GAGCTC 1 cut(s) 49
Eco105I TACGTA 1 cut(s) 221
Eco130I CCWWGG 1 cut(s) 1842
Eco24I GRGCYC 3 cut(s) 51, 559, 1842
Eco47I GGWCC 1 cut(s) 538
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 4 cut(s) 242, 1413, 1721, 1899
EcoICRI GAGCTC 1 cut(s) 49
EcoRI GAATTC 2 cut(s) 798, 1673
EcoRII CCWGG 3 cut(s) 23, 708, 1081
EcoT14I CCWWGG 1 cut(s) 1842
EcoT22I ATGCAT 1 cut(s) 1579
EcoT38I GRGCYC 3 cut(s) 51, 559, 1842
ErhI CCWWGG 1 cut(s) 1842
FaeI CATG 9 cut(s) 167, 208, 311, 325, 527, 538, 1048, 1070, 1521
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 1404, 1436
FatI CATG 9 cut(s) 163, 204, 307, 321, 523, 534, 1044, 1066, 1517
FauNDI CATATG 1 cut(s) 339
FbaI TGATCA 1 cut(s) 64
FblI GTMKAC 1 cut(s) 1486
Fnu4HI GCNGC 7 cut(s) 266, 741, 839, 981, 984, 1382, 1911
FokI GGATG 5 cut(s) 485, 1331, 1640, 1810, 1936
FriOI GRGCYC 3 cut(s) 51, 559, 1842
Fsp4HI GCNGC 7 cut(s) 266, 741, 839, 981, 984, 1382, 1911
FspBI CTAG 4 cut(s) 431, 1542, 1725, 1746
FspI TGCGCA 1 cut(s) 944
GlaI GCGC 1 cut(s) 944
GluI GCNGC 7 cut(s) 266, 741, 839, 981, 984, 1382, 1911
HaeIII GGCC 1 cut(s) 1759
HapII CCGG 3 cut(s) 658, 795, 1760
HgaI GACGC 1 cut(s) 1634
HhaI GCGC 1 cut(s) 945
Hin1II CATG 9 cut(s) 167, 208, 311, 325, 527, 538, 1048, 1070, 1521
Hin6I GCGC 1 cut(s) 943
HinP1I GCGC 1 cut(s) 943
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 3 cut(s) 1005, 1386, 1493
HinfI GANTC 7 cut(s) 33, 463, 590, 895, 1322, 1706, 1898
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 3 cut(s) 658, 795, 1760
HphI GGTGA 6 cut(s) 85, 601, 1409, 1421, 1928, 1948
Hpy166II GTNNAC 3 cut(s) 100, 1487, 1553
Hpy188I TCNGA 5 cut(s) 126, 628, 1333, 1432, 1692
Hpy188III TCNNGA 6 cut(s) 467, 524, 658, 803, 1529, 1863
Hpy8I GTNNAC 3 cut(s) 100, 1487, 1553
HpyAV CCTTC 5 cut(s) 49, 415, 876, 1696, 1933
HpyCH4III ACNGT 4 cut(s) 250, 1506, 1721, 1777
HpyCH4IV ACGT 4 cut(s) 220, 356, 579, 914
HpyF10VI GCNNNNNNNGC 4 cut(s) 540, 554, 942, 1651
HpyF3I CTNAG 3 cut(s) 50, 125, 1405
HpySE526I ACGT 4 cut(s) 220, 356, 579, 914
Hsp92II CATG 9 cut(s) 167, 208, 311, 325, 527, 538, 1048, 1070, 1521
HspAI GCGC 1 cut(s) 943
Kpn2I TCCGGA 1 cut(s) 657
Ksp22I TGATCA 1 cut(s) 64
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
LguI GCTCTTC 2 cut(s) 375, 1407
LmnI GCTCC 1 cut(s) 1445
Lsp1109I GCAGC 7 cut(s) 252, 727, 850, 967, 970, 1368, 1897
LweI GCATC 5 cut(s) 678, 945, 1324, 1564, 1829
MaeI CTAG 4 cut(s) 431, 1542, 1725, 1746
MaeII ACGT 4 cut(s) 220, 356, 579, 914
MaeIII GTNAC 3 cut(s) 607, 910, 1771
MalI GATC 7 cut(s) 22, 30, 66, 130, 675, 755, 963
MboI GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
MboII GAAGA 6 cut(s) 241, 392, 1419, 1424, 1582, 1892
MfeI CAATTG 2 cut(s) 132, 1649
MflI RGATCY 2 cut(s) 20, 961
MhlI GDGCHC 5 cut(s) 51, 559, 1732, 1782, 1842
MlyI GAGTC 1 cut(s) 457
MmeI TCCRAC 1 cut(s) 562
Mph1103I ATGCAT 1 cut(s) 1579
MroI TCCGGA 1 cut(s) 657
MspA1I CMGCKG 2 cut(s) 70, 493
MspCI CTTAAG 2 cut(s) 409, 1496
MspI CCGG 3 cut(s) 658, 795, 1760
MspR9I CCNGG 3 cut(s) 25, 710, 1083
MunI CAATTG 2 cut(s) 132, 1649
MvaI CCWGG 3 cut(s) 25, 710, 1083
MwoI GCNNNNNNNGC 4 cut(s) 540, 554, 942, 1651
NdeI CATATG 1 cut(s) 339
NdeII GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
NheI GCTAGC 1 cut(s) 1541
NlaIII CATG 9 cut(s) 167, 208, 311, 325, 527, 538, 1048, 1070, 1521
NmuCI GTSAC 2 cut(s) 607, 910
NsbI TGCGCA 1 cut(s) 944
NsiI ATGCAT 1 cut(s) 1579
NspI RCATGY 3 cut(s) 311, 325, 1048
PagI TCATGA 1 cut(s) 523
PciI ACATGT 2 cut(s) 321, 1044
PciSI GCTCTTC 2 cut(s) 375, 1407
PfeI GAWTC 6 cut(s) 33, 590, 895, 1322, 1706, 1898
PkrI GCNGC 7 cut(s) 267, 742, 840, 982, 985, 1383, 1912
PleI GAGTC 1 cut(s) 457
PpsI GAGTC 1 cut(s) 457
Ppu21I YACGTR 1 cut(s) 221
PscI ACATGT 2 cut(s) 321, 1044
Psp124BI GAGCTC 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 23, 708, 1081
PspGI CCWGG 3 cut(s) 23, 708, 1081
PspPI GGNCC 1 cut(s) 538
PstI CTGCAG 1 cut(s) 270
PstNI CAGNNNCTG 1 cut(s) 265
PsuI RGATCY 2 cut(s) 20, 961
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 7 cut(s) 219, 359, 552, 1263, 1554, 1712, 1954
RsaNI GTAC 7 cut(s) 218, 358, 551, 1262, 1553, 1711, 1953
SacI GAGCTC 1 cut(s) 51
SapI GCTCTTC 2 cut(s) 375, 1407
SatI GCNGC 7 cut(s) 266, 741, 839, 981, 984, 1382, 1911
Sau3AI GATC 7 cut(s) 20, 28, 64, 128, 673, 753, 961
Sau96I GGNCC 1 cut(s) 538
SchI GAGTC 1 cut(s) 457
ScrFI CCNGG 3 cut(s) 25, 710, 1083
SduI GDGCHC 5 cut(s) 51, 559, 1732, 1782, 1842
SfaNI GCATC 5 cut(s) 678, 945, 1324, 1564, 1829
SfcI CTRYAG 1 cut(s) 266
SinI GGWCC 1 cut(s) 538
SmlI CTYRAG 4 cut(s) 409, 465, 1444, 1496
SmoI CTYRAG 4 cut(s) 409, 465, 1444, 1496
SnaBI TACGTA 1 cut(s) 221
SsiI CCGC 4 cut(s) 137, 491, 541, 1613
SspMI CTAG 4 cut(s) 431, 1542, 1725, 1746
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 23, 708, 1081
StyI CCWWGG 1 cut(s) 1842
TaaI ACNGT 4 cut(s) 250, 1506, 1721, 1777
TaiI ACGT 4 cut(s) 223, 359, 582, 917
TaqI TCGA 5 cut(s) 31, 42, 672, 756, 1864
TatI WGTACW 3 cut(s) 1261, 1552, 1952
TfiI GAWTC 6 cut(s) 33, 590, 895, 1322, 1706, 1898
TscAI CASTG 7 cut(s) 550, 671, 784, 1474, 1591, 1782, 1882
TseFI GTSAC 2 cut(s) 607, 910
TseI GCWGC 7 cut(s) 265, 740, 838, 980, 983, 1381, 1910
Tsp45I GTSAC 2 cut(s) 607, 910
TspGWI ACGGA 2 cut(s) 987, 1467
TspRI CASTG 7 cut(s) 550, 671, 784, 1474, 1591, 1782, 1882
Vha464I CTTAAG 2 cut(s) 409, 1496
VpaK11BI GGWCC 1 cut(s) 538
XapI RAATTY 8 cut(s) 78, 182, 650, 798, 1117, 1460, 1603, 1673
XceI RCATGY 3 cut(s) 311, 325, 1048
XmiI GTMKAC 1 cut(s) 1486
XspI CTAG 4 cut(s) 431, 1542, 1725, 1746
Zsp2I ATGCAT 1 cut(s) 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.