Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0006121

Physical Location & Seq

Genomic Coordinates Reverse (-)

50371931 .. 50383173

Gene Structure 11243 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009295

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGTGGTTTTCGTTTTGGAGATCCAGGGATCGATTCTCCTTCGATGAGCTCAGGTATTTAACTGATCAGCTGGTGAAAATTCAAGTTGTGAATGACGTTAACAAGGATTTCGTTATTGAAGCTCTGAGATCAATTGCGGAGTTGATAACTTATGGCGACCAGCATGACACAACTTTTTTTGAATTTTTTATGGAGAAGCAAATCATGGGAGAGTTTGTACGTATATTGAAGATTAGCAGAAATATGACTGTTTCACTTCAGTTGCTGCAGACAATCAGCATTATGATACAGAACCTAAAAAATGAACATGCTATATATTACATGTTCAGTAATGAACATATGAACTACTTAATAACTTACTCATTTGATTTTAAGAATGAAGAGCTACTTTCTTACTATATATCCTTCTTAAGAGCAATAAGTGGAAAGCTAGATAGAAATACAATTTCTCTGCTTGTAAAGACTCAAGAGGATGAAGTTGTTTCATTTCCGCTGTATGTTGAGGCAATACGATTTGCTTTTCATGAGGAGAGCATGGTCCGCACTGCTGTACGGGCTCTGACGCTTAATGTTTATCACGTTGGAGATGATTCTGTCAATAGATATGTCACCAGTGCCCCTCATTCAGATTATTTTTCAAACTTGGTTAAATTTTTCCAGAAGCAGTGCGTCGATCTAAATGTATTGGTTTCTGACACTACGACAAACCTGGGTGCAGACACAACCTCGTCAATTCCTGCTGCTGTTGATGAGATCGAGGACAATCTCTACTATTTCAGTGATATTATTTCTGCGGGAATTCCTGATGTTGGGAGGTTATTCACAGACAACATATTGCAGCTTTTGATATTTCCATTGCTTCTACCTTCTCTAACACTAAAAGCTGTCAAGGGGATGGGTGCTGTCACGTCTTTGTATTTGATTTGTTGCATCTTGCGCATAGTAAAAATCAAGGATCTGTCAAATACTATTGCTGCTGCCCTATTCTGTCCGTTAGAAGCTTTCTTATCAAAGTCTGTTGCTGAACCCAATGGCTACATGTCTGGTTATGATGTTGCCCATGAAAGCCAACCACCAGGCGGTAATAATCTAACTAAGGCAGAAGTGGGAAATTTAAGTGTTGATGTGGCAAACTTGTCAATCTCTCCCCAAATCCAGCCAGTTGATGTCAGTATTGCAACAGGAAATGATAGTTGTGATTGTCATTTGCCTTTGAGGGAGGCTTTGCTTTCTTACCTTACGAGCGGGAACGATGTACAAGTTTCGGGTTCATTGAGTGTGCTGGCTACTTTGTTGCAGACAAAAGAATTGGATGAATCAATGTCAGATGCTCTGGGAATTCTTCCTCAACGCAAACAGCATAAGAAACTTCTGCTGCAAGCTTTGGTTGGTGAAAGCTCAGGTGAAGAGCAACTTTTTTCTTCAGAAAGTGGCTCCTTGAGAAATGGAATTGAATTTGGCAGTGAGCTTGATGGCTGTCTACAAAAGCTTAAGGAACAGTATGGAGTATCATGTTCTTTTCTGGAAGTGAAAGCTAGCCCTCGTGTACATAGATTTCAGGTGCTCGATGCATTGGTCAATGTCTTTGGCCGTTCAAATTTATCGGCGGAGACTTTATGGGATGGAGGTTGGCTTTTGCGTCAGTTACTTCCTTATAGTGAGGCAGAATTCAACAGCCATCATCGGAAGTTGCTGAAGGAATCGTACAAGAACTGTGCTAGTGCTCTTATTGAGGAGACTAGAGGAATCTGGCCGGATATACTTATAACAGTGCTCTGTGATAAATGGAAAAAGTGCAAGAGAGGAATAGAGGCATCATCCCCTCGGAAAGAGCCCAAGGACATCTTGTTTTCATCTCGAAAGTTCTCCACTGAAGATGGTATCACAAGCGATTCATCGTTTGCTGCTGGTGAAAGGATGTGTGAGTTGGTGAAGGTGTTTGTACTTCTACATCAACTTCAAATTTTCACCCTTGGTAGACCTTTGCCTGAGCAACCTCCTATTTATCCTCCAGCTGACCTTTTAGAAAACTCTCGTGCAAAGAGTGCTGGTGTAGATGCTTCAGGTCCCAAGCTTGGTAATGAGCTAAGACTTGTTGATGCCGTGCCTTGTAGAATTGCGTTTGAGAGGGGTAAAGAACGTCACTTTTGCTTTCTAGCAATCTCTTTGGGAGAATCAGGTTGGGTTGTTTTAGCTGAAGAATTACCCTTGAAGCAGGATTATGGTGTCGTTCGTGTGGCTGCACCTTTGGCTGGTTGCAATCCAAAAATTGATGATAAACATTCGAAATGGTTACACTTGCGGATCCGTCCATATACTCTACCCTCAATGGATCCTGCTAGATCTGGTGCATATGGGAAAGTGAAGACAAAAGCTTTGGTTGATGGGAGATGGACTCTTGCATTCAGGGATGAAGAATCTTGCAAATCTGCTTTGGCTATGATTTCGGAGGAGTTAAATCTACAAAGTAATGAGGTGGACAGAAGACTAAAACCTTTACTTGACCTCGAAACTGTTGTAGAATCTTCAAACCCTTCTCTAGGTCCCCCCGAGGCTTCCTCTTCTTATTCAACACCTTCCAATTCCTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0007034 GO:0008150 GO:0008152 GO:0009058 GO:0009718 GO:0009812 GO:0009813 GO:0009987 GO:0010638 GO:0012505 GO:0016020 GO:0016192 GO:0019898 GO:0030154 GO:0032502 GO:0033043 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044088 GO:0044090 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045165 GO:0046148 GO:0046283 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0065007 GO:0071702 GO:0071704 GO:1901576 GO:1903415

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FPL

Protein Analysis

864

Amino Acids

96.44

Weight (kDa)

5.43

Isoelectric Point (pI)

42.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FPL	PF09758	42 - 192	7.6e-55	Uncharacterised conserved protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006121)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28430
fragaria_vesca	FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030
malus_domestica	MD13G1240300.v1.1
prunus_persica	Prupe.1G073600_v2.0.a1 Prupe.1G073600_v2.0.a1 Prupe.1G073600_v2.0.a1
pyrus_communis	pycom13g21280 pycom16g20730
rosa_chinensis	RchiOBHm_Chr4g0399151
rosa_laevigata	RLG00000009295
rosa_multiflora	Rmu_sc0005882.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344120
rosa_rugosa	Rorug04G0014200
rosa_samantha	Rh4AG092100 Rh4BG090200 Rh4CG100600 Rh4DG084700
rosa_wichuraiana	Rw4G007610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1766
Acc16I	TGCGCA	1 cut(s)	938
AccBSI	CCGCTC	1 cut(s)	1245
AccI	GTMKAC	2 cut(s)	1480, 1978
AciI	CCGC	8 cut(s)	137, 491, 541, 794, 1080, 1245, 1607, 2302
AclWI	GGATC	7 cut(s)	15, 36, 963, 2299, 2312, 2327, 2340
AcoI	YGGCCR	2 cut(s)	1588, 1751
AcuI	CTGAAG	6 cut(s)	242, 1407, 1715, 1893, 2046, 2217
AfaI	GTAC	6 cut(s)	219, 552, 1257, 1548, 1706, 1944
AfiI	CCNNNNNNNGG	5 cut(s)	809, 1079, 2075, 2252, 2540
AflII	CTTAAG	2 cut(s)	409, 1490
AflIII	ACRYGT	2 cut(s)	321, 1038
AjiI	CACGTC	1 cut(s)	909
AjnI	CCWGG	3 cut(s)	23, 708, 1075
Alw21I	GWGCWC	4 cut(s)	51, 1566, 1726, 1776
Alw26I	GTCTC	2 cut(s)	1604, 1730
AlwI	GGATC	7 cut(s)	15, 36, 963, 2299, 2312, 2327, 2340
AlwNI	CAGNNNCTG	1 cut(s)	265
Ama87I	CYCGRG	1 cut(s)	2549
AoxI	GGCC	2 cut(s)	1588, 1751
ApeKI	GCWGC	8 cut(s)	265, 740, 838, 974, 977, 1375, 1904, 2240
ArsI	GACNNNNNNTTYG	2 cut(s)	2359, 2391
AspLEI	GCGC	1 cut(s)	939
AspS9I	GGNCC	3 cut(s)	538, 2066, 2543
AsuHPI	GGTGA	7 cut(s)	85, 601, 1403, 1415, 1922, 1942, 1960
AsuII	TTCGAA	1 cut(s)	2285
AsuNHI	GCTAGC	1 cut(s)	1535
AvaI	CYCGRG	1 cut(s)	2549
AvaII	GGWCC	3 cut(s)	538, 2066, 2543
BaeGI	GKGCMC	1 cut(s)	619
BamHI	GGATCC	2 cut(s)	2304, 2332
BanII	GRGCYC	3 cut(s)	51, 559, 1836
BarI	GAAGNNNNNNTAC	2 cut(s)	1688, 1720
BauI	CACGAG	2 cut(s)	1542, 2034
BbsI	GAAGAC	2 cut(s)	2372, 2491
Bbv12I	GWGCWC	4 cut(s)	51, 1566, 1726, 1776
BbvI	GCAGC	8 cut(s)	252, 727, 850, 961, 964, 1362, 1891, 2227
BccI	CCATC	7 cut(s)	889, 1466, 1616, 1686, 1871, 2378, 2385
BceAI	ACGGC	2 cut(s)	1575, 2087
BciT130I	CCWGG	3 cut(s)	25, 710, 1077
BclI	TGATCA	1 cut(s)	64
BcoDI	GTCTC	2 cut(s)	1604, 1730
BfaI	CTAG	7 cut(s)	431, 1536, 1719, 1740, 2156, 2340, 2540
BfmI	CTRYAG	1 cut(s)	266
BfrI	CTTAAG	2 cut(s)	409, 1490
BglII	AGATCT	1 cut(s)	2342
BisI	GCNGC	8 cut(s)	266, 741, 839, 975, 978, 1376, 1905, 2241
BlsI	GCNGC	8 cut(s)	267, 742, 840, 976, 979, 1377, 1906, 2242
Bme1390I	CCNGG	3 cut(s)	25, 710, 1077
Bme18I	GGWCC	3 cut(s)	538, 2066, 2543
BmeT110I	CYCGRG	1 cut(s)	2549
BmgBI	CACGTC	1 cut(s)	909
BmgT120I	GGNCC	3 cut(s)	538, 2066, 2543
BmiI	GGNNCC	5 cut(s)	1435, 2068, 2306, 2334, 2545
BmrFI	CCNGG	3 cut(s)	25, 710, 1077
BmsI	GCATC	6 cut(s)	939, 1318, 1558, 1823, 2047, 2089
BmtI	GCTAGC	1 cut(s)	1539
BpiI	GAAGAC	2 cut(s)	2372, 2491
BplI	GAGNNNNNCTC	4 cut(s)	2380, 2412, 2543, 2575
BpmI	CTGGAG	1 cut(s)	1995
Bpu10I	CCTNAGC	3 cut(s)	50, 1399, 1989
Bpu14I	TTCGAA	1 cut(s)	2285
BpuEI	CTTGAG	2 cut(s)	450, 1459
Bsa29I	ATCGAT	1 cut(s)	31
BsaAI	YACGTR	1 cut(s)	221
BsaJI	CCNNGG	6 cut(s)	24, 709, 1823, 1836, 1972, 2550
BsaXI	ACNNNNNCTCC	2 cut(s)	201, 231
Bsc4I	CCNNNNNNNGG	5 cut(s)	809, 1079, 2075, 2252, 2540
Bse1I	ACTGG	2 cut(s)	612, 1160
Bse3DI	GCAATG	1 cut(s)	854
BseBI	CCWGG	3 cut(s)	25, 710, 1077
BseCI	ATCGAT	1 cut(s)	31
BseDI	CCNNGG	6 cut(s)	24, 709, 1823, 1836, 1972, 2550
BseGI	GGATG	7 cut(s)	478, 900, 1318, 1627, 1817, 1923, 2416
BseLI	CCNNNNNNNGG	5 cut(s)	809, 1079, 2075, 2252, 2540
BseMI	GCAATG	1 cut(s)	854
BseMII	CTCAG	4 cut(s)	64, 116, 1413, 1980
BseNI	ACTGG	2 cut(s)	612, 1160
BseRI	GAGGAG	3 cut(s)	542, 1748, 2465
BseSI	GKGCMC	1 cut(s)	619
BseXI	GCAGC	8 cut(s)	252, 727, 850, 961, 964, 1362, 1891, 2227
BsgI	GTGCAG	2 cut(s)	735, 2226
BshFI	GGCC	2 cut(s)	1590, 1753
BshVI	ATCGAT	1 cut(s)	31
BsiHKAI	GWGCWC	4 cut(s)	51, 1566, 1726, 1776
BsiHKCI	CYCGRG	1 cut(s)	2549
BsiSI	CCGG	1 cut(s)	1754
BslFI	GGGAC	2 cut(s)	2052, 2529
BslI	CCNNNNNNNGG	5 cut(s)	809, 1079, 2075, 2252, 2540
BsmAI	GTCTC	2 cut(s)	1604, 1730
BsmFI	GGGAC	2 cut(s)	2052, 2529
BsmI	GAATGC	1 cut(s)	2402
BsnI	GGCC	2 cut(s)	1590, 1753
BsoBI	CYCGRG	1 cut(s)	2549
Bsp119I	TTCGAA	1 cut(s)	2285
Bsp1286I	GDGCHC	7 cut(s)	51, 559, 619, 1566, 1726, 1776, 1836
Bsp1407I	TGTACA	2 cut(s)	1255, 1546
BspACI	CCGC	8 cut(s)	137, 491, 541, 794, 1080, 1245, 1607, 2302
BspANI	GGCC	2 cut(s)	1590, 1753
BspCNI	CTCAG	4 cut(s)	63, 117, 1412, 1981
BspDI	ATCGAT	1 cut(s)	31
BspHI	TCATGA	1 cut(s)	523
BspLI	GGNNCC	5 cut(s)	1435, 2068, 2306, 2334, 2545
BspMAI	CTGCAG	1 cut(s)	270
BspOI	GCTAGC	1 cut(s)	1539
BspPI	GGATC	7 cut(s)	15, 36, 963, 2299, 2312, 2327, 2340
BspQI	GCTCTTC	2 cut(s)	375, 1401
BspT104I	TTCGAA	1 cut(s)	2285
BspTI	CTTAAG	2 cut(s)	409, 1490
BsrBI	CCGCTC	1 cut(s)	1245
BsrDI	GCAATG	1 cut(s)	854
BsrGI	TGTACA	2 cut(s)	1255, 1546
BsrI	ACTGG	2 cut(s)	612, 1160
BssECI	CCNNGG	6 cut(s)	24, 709, 1823, 1836, 1972, 2550
BssSI	CACGAG	2 cut(s)	1542, 2034
BssT1I	CCWWGG	2 cut(s)	1836, 1972
Bst2BI	CACGAG	2 cut(s)	1542, 2034
Bst2UI	CCWGG	3 cut(s)	25, 710, 1077
Bst4CI	ACNGT	5 cut(s)	250, 1500, 1715, 1771, 2515
Bst6I	CTCTTC	3 cut(s)	375, 1401, 2566
BstAFI	CTTAAG	2 cut(s)	409, 1490
BstAPI	GCANNNNNTGC	1 cut(s)	2045
BstAUI	TGTACA	2 cut(s)	1255, 1546
BstBAI	YACGTR	1 cut(s)	221
BstBI	TTCGAA	1 cut(s)	2285
BstC8I	GCNNGC	3 cut(s)	1284, 1380, 1537
BstDEI	CTNAG	6 cut(s)	50, 125, 1095, 1399, 1989, 2087
BstENI	CCTNNNNNAGG	1 cut(s)	2538
BstF5I	GGATG	7 cut(s)	478, 900, 1318, 1627, 1817, 1923, 2416
BstHHI	GCGC	1 cut(s)	939
BstMAI	GTCTC	2 cut(s)	1604, 1730
BstMWI	GCNNNNNNNGC	5 cut(s)	540, 554, 936, 2045, 2249
BstNI	CCWGG	3 cut(s)	25, 710, 1077
BstNSI	RCATGY	3 cut(s)	311, 325, 1042
BstSCI	CCNGG	3 cut(s)	23, 708, 1075
BstSFI	CTRYAG	1 cut(s)	266
BstSLI	GKGCMC	1 cut(s)	619
BstSNI	TACGTA	1 cut(s)	221
BstV1I	GCAGC	8 cut(s)	252, 727, 850, 961, 964, 1362, 1891, 2227
BstV2I	GAAGAC	2 cut(s)	2372, 2491
BstX2I	RGATCY	5 cut(s)	20, 955, 2304, 2332, 2342
BstYI	RGATCY	5 cut(s)	20, 955, 2304, 2332, 2342
Bsu15I	ATCGAT	1 cut(s)	31
BsuRI	GGCC	2 cut(s)	1590, 1753
BsuTUI	ATCGAT	1 cut(s)	31
BtrI	CACGTC	1 cut(s)	909
BtsCI	GGATG	7 cut(s)	478, 900, 1318, 1627, 1817, 1923, 2416
BtsI	GCAGTG	3 cut(s)	543, 671, 1468
BtsIMutI	CAGTG	7 cut(s)	543, 619, 671, 784, 1468, 1776, 1869
Cac8I	GCNNGC	3 cut(s)	1284, 1380, 1537
CaiI	CAGNNNCTG	1 cut(s)	265
CciI	TCATGA	1 cut(s)	523
CfoI	GCGC	1 cut(s)	939
Cfr13I	GGNCC	3 cut(s)	538, 2066, 2543
ClaI	ATCGAT	1 cut(s)	31
CseI	GACGC	3 cut(s)	571, 658, 1628
Csp6I	GTAC	6 cut(s)	218, 551, 1256, 1547, 1705, 1943
CviAII	CATG	9 cut(s)	164, 205, 308, 322, 524, 535, 1039, 1061, 1512
CviQI	GTAC	6 cut(s)	218, 551, 1256, 1547, 1705, 1943
DdeI	CTNAG	6 cut(s)	50, 125, 1095, 1399, 1989, 2087
EaeI	YGGCCR	2 cut(s)	1588, 1751
Eam1104I	CTCTTC	3 cut(s)	375, 1401, 2566
EarI	CTCTTC	3 cut(s)	375, 1401, 2566
EciI	GGCGGA	1 cut(s)	1622
Ecl136II	GAGCTC	1 cut(s)	49
Eco105I	TACGTA	1 cut(s)	221
Eco130I	CCWWGG	2 cut(s)	1836, 1972
Eco24I	GRGCYC	3 cut(s)	51, 559, 1836
Eco47I	GGWCC	3 cut(s)	538, 2066, 2543
Eco53kI	GAGCTC	1 cut(s)	49
Eco57I	CTGAAG	6 cut(s)	242, 1407, 1715, 1893, 2046, 2217
Eco88I	CYCGRG	1 cut(s)	2549
EcoICRI	GAGCTC	1 cut(s)	49
EcoNI	CCTNNNNNAGG	1 cut(s)	2538
EcoO109I	RGGNCCY	2 cut(s)	2066, 2543
EcoRI	GAATTC	3 cut(s)	798, 1338, 1667
EcoRII	CCWGG	3 cut(s)	23, 708, 1075
EcoT14I	CCWWGG	2 cut(s)	1836, 1972
EcoT22I	ATGCAT	1 cut(s)	1573
EcoT38I	GRGCYC	3 cut(s)	51, 559, 1836
ErhI	CCWWGG	2 cut(s)	1836, 1972
FaeI	CATG	9 cut(s)	167, 208, 311, 325, 527, 538, 1042, 1064, 1515
FalI	AAGNNNNNCTT	6 cut(s)	372, 404, 1398, 1430, 1829, 1861
FaqI	GGGAC	2 cut(s)	2052, 2529
FatI	CATG	9 cut(s)	163, 204, 307, 321, 523, 534, 1038, 1060, 1511
FauI	CCCGC	2 cut(s)	787, 1238
FauNDI	CATATG	2 cut(s)	339, 2353
FbaI	TGATCA	1 cut(s)	64
FblI	GTMKAC	2 cut(s)	1480, 1978
Fnu4HI	GCNGC	8 cut(s)	266, 741, 839, 975, 978, 1376, 1905, 2241
FokI	GGATG	7 cut(s)	485, 907, 1325, 1634, 1804, 1930, 2423
FriOI	GRGCYC	3 cut(s)	51, 559, 1836
Fsp4HI	GCNGC	8 cut(s)	266, 741, 839, 975, 978, 1376, 1905, 2241
FspBI	CTAG	7 cut(s)	431, 1536, 1719, 1740, 2156, 2340, 2540
FspI	TGCGCA	1 cut(s)	938
GlaI	GCGC	1 cut(s)	938
GluI	GCNGC	8 cut(s)	266, 741, 839, 975, 978, 1376, 1905, 2241
GsuI	CTGGAG	1 cut(s)	1995
HaeIII	GGCC	2 cut(s)	1590, 1753
HapII	CCGG	1 cut(s)	1754
HgaI	GACGC	3 cut(s)	571, 658, 1628
HhaI	GCGC	1 cut(s)	939
Hin1II	CATG	9 cut(s)	167, 208, 311, 325, 527, 538, 1042, 1064, 1515
Hin6I	GCGC	1 cut(s)	937
HinP1I	GCGC	1 cut(s)	937
HincII	GTYRAC	1 cut(s)	100
HindII	GTYRAC	1 cut(s)	100
HindIII	AAGCTT	5 cut(s)	999, 1380, 1487, 2072, 2373
HpaI	GTTAAC	1 cut(s)	100
HpaII	CCGG	1 cut(s)	1754
HphI	GGTGA	7 cut(s)	85, 601, 1403, 1415, 1922, 1942, 1960
Hpy166II	GTNNAC	5 cut(s)	100, 1481, 1547, 1979, 2479
Hpy188I	TCNGA	9 cut(s)	126, 561, 628, 694, 1327, 1426, 1686, 1827, 2449
Hpy188III	TCNNGA	6 cut(s)	467, 524, 658, 803, 1523, 1857
Hpy8I	GTNNAC	5 cut(s)	100, 1481, 1547, 1979, 2479
Hpy99I	CGWCG	1 cut(s)	674
HpyAV	CCTTC	7 cut(s)	49, 415, 876, 1690, 1927, 2544, 2586
HpyCH4III	ACNGT	5 cut(s)	250, 1500, 1715, 1771, 2515
HpyCH4IV	ACGT	5 cut(s)	96, 220, 579, 908, 2140
HpyF10VI	GCNNNNNNNGC	5 cut(s)	540, 554, 936, 2045, 2249
HpyF3I	CTNAG	6 cut(s)	50, 125, 1095, 1399, 1989, 2087
HpySE526I	ACGT	5 cut(s)	96, 220, 579, 908, 2140
Hsp92II	CATG	9 cut(s)	167, 208, 311, 325, 527, 538, 1042, 1064, 1515
HspAI	GCGC	1 cut(s)	937
Ksp22I	TGATCA	1 cut(s)	64
KspAI	GTTAAC	1 cut(s)	100
LguI	GCTCTTC	2 cut(s)	375, 1401
LmnI	GCTCC	1 cut(s)	1439
Lsp1109I	GCAGC	8 cut(s)	252, 727, 850, 961, 964, 1362, 1891, 2227
LweI	GCATC	6 cut(s)	939, 1318, 1558, 1823, 2047, 2089
MaeI	CTAG	7 cut(s)	431, 1536, 1719, 1740, 2156, 2340, 2540
MaeII	ACGT	5 cut(s)	96, 220, 579, 908, 2140
MaeIII	GTNAC	5 cut(s)	607, 904, 1644, 2141, 2292
MbiI	CCGCTC	1 cut(s)	1245
MfeI	CAATTG	1 cut(s)	132
MflI	RGATCY	5 cut(s)	20, 955, 2304, 2332, 2342
MhlI	GDGCHC	7 cut(s)	51, 559, 619, 1566, 1726, 1776, 1836
MlyI	GAGTC	2 cut(s)	457, 2389
MmeI	TCCRAC	1 cut(s)	562
Mph1103I	ATGCAT	1 cut(s)	1573
MspA1I	CMGCKG	3 cut(s)	70, 493, 2015
MspCI	CTTAAG	2 cut(s)	409, 1490
MspI	CCGG	1 cut(s)	1754
MspR9I	CCNGG	3 cut(s)	25, 710, 1077
MunI	CAATTG	1 cut(s)	132
Mva1269I	GAATGC	1 cut(s)	2402
MvaI	CCWGG	3 cut(s)	25, 710, 1077
MwoI	GCNNNNNNNGC	5 cut(s)	540, 554, 936, 2045, 2249
NdeI	CATATG	2 cut(s)	339, 2353
NheI	GCTAGC	1 cut(s)	1535
NlaIII	CATG	9 cut(s)	167, 208, 311, 325, 527, 538, 1042, 1064, 1515
NlaIV	GGNNCC	5 cut(s)	1435, 2068, 2306, 2334, 2545
NmuCI	GTSAC	3 cut(s)	607, 904, 2141
NsbI	TGCGCA	1 cut(s)	938
NsiI	ATGCAT	1 cut(s)	1573
NspI	RCATGY	3 cut(s)	311, 325, 1042
NspV	TTCGAA	1 cut(s)	2285
PagI	TCATGA	1 cut(s)	523
PciI	ACATGT	2 cut(s)	321, 1038
PciSI	GCTCTTC	2 cut(s)	375, 1401
PctI	GAATGC	1 cut(s)	2402
PfeI	GAWTC	9 cut(s)	33, 590, 1316, 1700, 1746, 1892, 2174, 2417, 2522
PkrI	GCNGC	8 cut(s)	267, 742, 840, 976, 979, 1377, 1906, 2242
PleI	GAGTC	2 cut(s)	457, 2389
PpsI	GAGTC	2 cut(s)	457, 2389
Ppu21I	YACGTR	1 cut(s)	221
PpuMI	RGGWCCY	2 cut(s)	2066, 2543
PscI	ACATGT	2 cut(s)	321, 1038
PsiI	TTATAA	1 cut(s)	1766
Psp124BI	GAGCTC	1 cut(s)	51
Psp5II	RGGWCCY	2 cut(s)	2066, 2543
Psp6I	CCWGG	3 cut(s)	23, 708, 1075
PspGI	CCWGG	3 cut(s)	23, 708, 1075
PspN4I	GGNNCC	5 cut(s)	1435, 2068, 2306, 2334, 2545
PspPI	GGNCC	3 cut(s)	538, 2066, 2543
PspPPI	RGGWCCY	2 cut(s)	2066, 2543
PstI	CTGCAG	1 cut(s)	270
PstNI	CAGNNNCTG	1 cut(s)	265
PsuI	RGATCY	5 cut(s)	20, 955, 2304, 2332, 2342
PvuII	CAGCTG	2 cut(s)	70, 2015
RsaI	GTAC	6 cut(s)	219, 552, 1257, 1548, 1706, 1944
RsaNI	GTAC	6 cut(s)	218, 551, 1256, 1547, 1705, 1943
SacI	GAGCTC	1 cut(s)	51
SapI	GCTCTTC	2 cut(s)	375, 1401
SatI	GCNGC	8 cut(s)	266, 741, 839, 975, 978, 1376, 1905, 2241
Sau96I	GGNCC	3 cut(s)	538, 2066, 2543
SchI	GAGTC	2 cut(s)	457, 2389
ScrFI	CCNGG	3 cut(s)	25, 710, 1077
SduI	GDGCHC	7 cut(s)	51, 559, 619, 1566, 1726, 1776, 1836
SfaNI	GCATC	6 cut(s)	939, 1318, 1558, 1823, 2047, 2089
SfcI	CTRYAG	1 cut(s)	266
SfuI	TTCGAA	1 cut(s)	2285
SinI	GGWCC	3 cut(s)	538, 2066, 2543
SmlI	CTYRAG	4 cut(s)	409, 465, 1438, 1490
SmoI	CTYRAG	4 cut(s)	409, 465, 1438, 1490
SnaBI	TACGTA	1 cut(s)	221
SsiI	CCGC	8 cut(s)	137, 491, 541, 794, 1080, 1245, 1607, 2302
SspMI	CTAG	7 cut(s)	431, 1536, 1719, 1740, 2156, 2340, 2540
SstI	GAGCTC	1 cut(s)	51
StyD4I	CCNGG	3 cut(s)	23, 708, 1075
StyI	CCWWGG	2 cut(s)	1836, 1972
TaaI	ACNGT	5 cut(s)	250, 1500, 1715, 1771, 2515
TaiI	ACGT	5 cut(s)	99, 223, 582, 911, 2143
TaqI	TCGA	8 cut(s)	31, 42, 672, 756, 1566, 1858, 2285, 2508
TatI	WGTACW	3 cut(s)	1255, 1546, 1942
TfiI	GAWTC	9 cut(s)	33, 590, 1316, 1700, 1746, 1892, 2174, 2417, 2522
TscAI	CASTG	7 cut(s)	550, 619, 671, 784, 1468, 1776, 1876
TseFI	GTSAC	3 cut(s)	607, 904, 2141
TseI	GCWGC	8 cut(s)	265, 740, 838, 974, 977, 1375, 1904, 2240
Tsp45I	GTSAC	3 cut(s)	607, 904, 2141
TspGWI	ACGGA	2 cut(s)	981, 2297
TspRI	CASTG	7 cut(s)	550, 619, 671, 784, 1468, 1776, 1876
Vha464I	CTTAAG	2 cut(s)	409, 1490
VpaK11BI	GGWCC	3 cut(s)	538, 2066, 2543
XagI	CCTNNNNNAGG	1 cut(s)	2538
XceI	RCATGY	3 cut(s)	311, 325, 1042
XmiI	GTMKAC	2 cut(s)	1480, 1978
XspI	CTAG	7 cut(s)	431, 1536, 1719, 1740, 2156, 2340, 2540
Zsp2I	ATGCAT	1 cut(s)	1573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.