Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0006121

Physical Location & Seq

Genomic Coordinates Forward (+)

18629087 .. 18642973

Gene Structure 13887 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g20730.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGTGGTTTTCGTTTTGGAAGTCCAGGGATCGCTTCTCCTTAGACGAGCTCAGGTATTTAACTGATCAGCTTATAAAACTTCAAACTGTGAATGAAGTAAACAAGGATTTCGTTGTTGAGGCGCTGAGATCCATTGCAGAGTTAATAACGTATGGAGACCAGCATGACTCTGCCTTTTTTGAATTTTTCATGGAGAAGCAAGTCATGGGAGAGTTTGTACGTATATTGAAGGTTAGCAGAACTCTGACTGTGTCACTTCAGTTGCTACAGACAATCAGCATTATGATCCAGAACCTAAAAAATGAACACGCTATATACTACTTGTTCAGTAATGAATATATGAACTACTTGATAACATATTCATTTGATTTCCAAAATGAAGAGCTACTGTCATACTATATATCCTTCTTAAGAGCAATAAGTGGGAAGCTAGACAGGAATACAATTTCTCTGCTTGTAAAAACTCAAAATGATGAAGTGGTTTCATTTCCACTCTATGATGAGGCAATACATTTTGCCTCTCATGAGGAGAGCATGGTCCGCACCGCCATACGGGCTTTGACGCTTAATGTTTATCATGTTGGAGATGAAAGTGTCAACAGATATGTCACCAGTGCCCCTCATGCAGATTATTTTTCAAACCTGGTTAAATTTTTCCGAAGGCAGTGCATCAATCTAAATGCATTGGTTTCTGACTCTACAAAAAACCCAGGTGCAGAGTCTGCAGACACAACCACATTAATTTCTACTGCTGTTGATGAGATCGAGGACAATCTCTACTATTTTAGCGATGTTATTTCTGCAGGAATTCCTGATGTTGGGTGGTTAATTACAGATAACATCTTGCAGCTTTTGATATTCCCACTGCTTCTTCCTTCGCTTACCATAAAAGCTGTCGAAGGAATACAAATTGGTGCTGCCACGTCTTTGTACTTAGTTTGTTGCATCCTGCGCATAGTGAAAATCAAGGATTTGGCAAATACCATTTCTACTGCTCTTTTCTGTCCGTTAGATGCTTTCTCTCCAGAGTCTGGAGCTAAACCAAATGGCCATACATCTTTTTATGGTTTTGCACATGAAAGTCAACCGCCAGGCAGTAATACCAAGGAAGGTGTTGGAATCTTAAGAGTTGACGTGCCAAACGAGTCAAGCTCTCCCCATATTCACCCAGTGGATGTTACAAGAGAAAATGATTATTGTGATTCTCATTTGTCTTTGAGGGAGGCTTTGATTTATTATATTATGAGTGGGGATGATATACAAGTTGCTGGTTCGTTAAGTGTACTGGCTACTTTACTGCAGACAAAAGAATTGGATGAATCAATACTAGATGCTCTTGGAATTCTTCCTCAATGCAAACAGCATAAGAAACTCCTGCTGCAAGCTTTGGTTGGTGAAGTCTCAGGAGAAGAGCAACTTTTTTCTTCAGAACGTAGCTCATCGAAAGATGGTACTGAATGTGGTAGTGAGCTTGATAGTTGTCTACAAAAGCTTAAGGAGCAGTTTGGACTGTTGTGTTCTTCTCTGGAAGTGAGGACTAGCCCTCGAGTACATAGATTTCAGGTTCTTGATGCATTGGTCAGTCTTTTTGGCCGTTCAAATATATCTGCAGAGACATTATGGGATGGTGGTTGGCTTTTGCGCCAGTTACTTCCTTATAGCGAGTCAGAGTTTAATAGCCATCATCTCAAGGTGCTGAATGATTCATATAAGAACTGTACCAGTGCTCTTCTCAAGGAGACTAGAGGAGTCTGGCCAGATCTTCTTGTAACAGTCCTTTGTGATGAATGGAAACTCTGCAAGAGAGCAATAGAGGCCTCCTCCCCTCGGAAAGAGCCCAAGTGTATTCTGTTGTCATCTCAGAGGTTCTTGTCTGAAGATGGTATCACAAGTGATTCATCGTTTGCTGCTGGTGAAAGGATGTGTGAGTTGGTCAAGGTGTTTGTACTCCTACATCAACTTCAAATTTTCTCACTTGGTAGAACTTTTCCTGAGAAACCTCCTATTAACCCTCCTGCTGATGTTTTTGAAAATTCCCGTGCACGAAGCGCTGGCCTAGATGCTGCAGGTCCAAAGCTTGGCACTGAGCTAACGCTTGTTGATGCTGTGCCTTGTAGAATTGCGTTTGAGAGGGGTAAAGAACGTCATTTTTCCTTTCTAGCAATCTCTTTGGGTACATCTGGGTGGGTTGTTTTAGCTGAAGAATTACCCCTAAAGGAGCCTTATGGAGTTGTTCGTATGGTTGCACCTTTGGCTGGTTCCAATTTCATATTGGTAAGCAGTACCCGATTCTTTCTGTTGAAGCCAAAACCTGATGATAAGCATTCAAGATGGTTACACTTGCGAATCCGTCCATCCACTTTACCATTTGTGGAGCCTGCCAAATATGGTGCCTACGGGAAATCGAAGACAAAAGCCTTGGTTGATGGAAGATGGACCCTAGCATTCAGGGACGAAGAATCTTGCAACACTGCTTTGTCTATGGTTTTGGAGGAGATAAACCTGCAAAGCAATGAGGTAGAGAGAAGATTAAAACCTTTACTTGACCTCAAAAGCTTAGTAGAATCTTCAAACTCTTCTCAAGGTCCTCGAACTTCCTCTTTTCATTCAACACTTTCTAATTCTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001708 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0007034 GO:0008150 GO:0008152 GO:0009058 GO:0009718 GO:0009812 GO:0009813 GO:0009987 GO:0010638 GO:0012505 GO:0016020 GO:0016192 GO:0019898 GO:0030154 GO:0032502 GO:0033043 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044088 GO:0044090 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045165 GO:0046148 GO:0046283 GO:0048518 GO:0048522 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051234 GO:0065007 GO:0071702 GO:0071704 GO:1901576 GO:1903415

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FPL

Protein Analysis

877

Amino Acids

98.25

Weight (kDa)

5.53

Isoelectric Point (pI)

43.74

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006121)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G28430
fragaria_vesca	FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030 FvH4_4g07030
malus_domestica	MD13G1240300.v1.1
prunus_persica	Prupe.1G073600_v2.0.a1 Prupe.1G073600_v2.0.a1 Prupe.1G073600_v2.0.a1
pyrus_communis	pycom13g21280 pycom16g20730
rosa_chinensis	RchiOBHm_Chr4g0399151
rosa_laevigata	RLG00000009295
rosa_multiflora	Rmu_sc0005882.1_g000001
rosa_roxburghii	Rroxscaffold_5G00344120
rosa_rugosa	Rorug04G0014200
rosa_samantha	Rh4AG092100 Rh4BG090200 Rh4CG100600 Rh4DG084700
rosa_wichuraiana	Rw4G007610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	74
Acc16I	TGCGCA	1 cut(s)	953
Acc36I	ACCTGC	2 cut(s)	2057, 2511
AccB1I	GGYRCC	1 cut(s)	2390
AccB7I	CCANNNNNTGG	2 cut(s)	1031, 2078
AccI	GTMKAC	1 cut(s)	1483
AciI	CCGC	3 cut(s)	541, 546, 1088
AclWI	GGATC	3 cut(s)	36, 123, 280
AcoI	YGGCCR	3 cut(s)	1048, 1591, 1754
AcsI	RAATTY	6 cut(s)	182, 650, 807, 1341, 1965, 2032
AcuI	CTGAAG	4 cut(s)	242, 1410, 1896, 2220
AdeI	CACNNNGTG	1 cut(s)	1171
AfaI	GTAC	9 cut(s)	219, 932, 1284, 1453, 1551, 1720, 1947, 2176, 2284
AfeI	AGCGCT	1 cut(s)	2050
AfiI	CCNNNNNNNGG	6 cut(s)	552, 818, 1031, 1827, 2078, 2255
AflII	CTTAAG	3 cut(s)	409, 1123, 1493
AjiI	CACGTC	2 cut(s)	924, 1135
AjnI	CCWGG	4 cut(s)	23, 642, 709, 1090
Alw21I	GWGCWC	3 cut(s)	51, 1729, 2044
Alw26I	GTCTC	4 cut(s)	150, 1405, 1607, 1733
Alw44I	GTGCAC	1 cut(s)	2040
AlwI	GGATC	3 cut(s)	36, 123, 280
AlwNI	CAGNNNCTG	2 cut(s)	722, 1031
Ama87I	CYCGRG	1 cut(s)	1545
Aor51HI	AGCGCT	1 cut(s)	2050
AoxI	GGCC	5 cut(s)	1048, 1591, 1754, 1815, 2053
ApaLI	GTGCAC	1 cut(s)	2040
ApeKI	GCWGC	5 cut(s)	847, 917, 1378, 1907, 2063
ApoI	RAATTY	6 cut(s)	182, 650, 807, 1341, 1965, 2032
AseI	ATTAAT	1 cut(s)	740
AspLEI	GCGC	4 cut(s)	124, 954, 1644, 2051
AspS9I	GGNCC	4 cut(s)	538, 2069, 2436, 2585
AsuHPI	GGTGA	4 cut(s)	601, 1157, 1406, 1925
AvaI	CYCGRG	1 cut(s)	1545
AvaII	GGWCC	4 cut(s)	538, 2069, 2436, 2585
BaeGI	GKGCMC	2 cut(s)	619, 2044
BalI	TGGCCA	2 cut(s)	1050, 1756
BanI	GGYRCC	1 cut(s)	2390
BanII	GRGCYC	2 cut(s)	51, 1839
BbsI	GAAGAC	1 cut(s)	2414
Bbv12I	GWGCWC	3 cut(s)	51, 1729, 2044
BbvI	GCAGC	5 cut(s)	859, 904, 1365, 1894, 2050
BccI	CCATC	8 cut(s)	1442, 1619, 1689, 1874, 2325, 2362, 2420, 2427
BceAI	ACGGC	1 cut(s)	1578
BcgI	CGANNNNNNTGC	2 cut(s)	648, 682
BciT130I	CCWGG	4 cut(s)	25, 644, 711, 1092
BclI	TGATCA	1 cut(s)	64
BcoDI	GTCTC	4 cut(s)	150, 1405, 1607, 1733
BfaI	CTAG	7 cut(s)	431, 1328, 1539, 1743, 2057, 2159, 2441
BfmI	CTRYAG	6 cut(s)	266, 723, 801, 1298, 1608, 2064
BfoI	RGCGCY	2 cut(s)	125, 2052
BfrI	CTTAAG	3 cut(s)	409, 1123, 1493
BfuAI	ACCTGC	2 cut(s)	2057, 2511
BglI	GCCNNNNNGGC	1 cut(s)	554
BglII	AGATCT	1 cut(s)	1759
BisI	GCNGC	5 cut(s)	848, 918, 1379, 1908, 2064
BlsI	GCNGC	5 cut(s)	849, 919, 1380, 1909, 2065
Bme1390I	CCNGG	4 cut(s)	25, 644, 711, 1092
Bme18I	GGWCC	4 cut(s)	538, 2069, 2436, 2585
BmeT110I	CYCGRG	1 cut(s)	1545
BmgBI	CACGTC	2 cut(s)	924, 1135
BmgT120I	GGNCC	4 cut(s)	538, 2069, 2436, 2585
BmiI	GGNNCC	5 cut(s)	2220, 2260, 2376, 2392, 2438
BmrFI	CCNGG	4 cut(s)	25, 644, 711, 1092
BmrI	ACTGGG	1 cut(s)	1163
BmsI	GCATC	7 cut(s)	678, 954, 1003, 1321, 1561, 2050, 2092
BmuI	ACTGGG	1 cut(s)	1163
BpiI	GAAGAC	1 cut(s)	2414
BplI	GAGNNNNNCTC	4 cut(s)	1136, 1168, 1805, 1837
BpmI	CTGGAG	2 cut(s)	1008, 1053
Bpu10I	CCTNAGC	1 cut(s)	50
BpuEI	CTTGAG	3 cut(s)	1673, 1718, 2565
BsaAI	YACGTR	1 cut(s)	221
BsaI	GGTCTC	1 cut(s)	150
BsaJI	CCNNGG	5 cut(s)	24, 709, 1104, 1826, 2418
BsaXI	ACNNNNNCTCC	2 cut(s)	201, 231
Bsc4I	CCNNNNNNNGG	6 cut(s)	552, 818, 1031, 1827, 2078, 2255
Bse1I	ACTGG	5 cut(s)	612, 1169, 1290, 1645, 1722
Bse3DI	GCAATG	2 cut(s)	132, 2518
BseBI	CCWGG	4 cut(s)	25, 644, 711, 1092
BseDI	CCNNGG	5 cut(s)	24, 709, 1104, 1826, 2418
BseGI	GGATG	7 cut(s)	945, 1180, 1258, 1321, 1630, 1926, 2354
BseLI	CCNNNNNNNGG	6 cut(s)	552, 818, 1031, 1827, 2078, 2255
BseMI	GCAATG	2 cut(s)	132, 2518
BseMII	CTCAG	6 cut(s)	64, 116, 1416, 1874, 1983, 2076
BseNI	ACTGG	5 cut(s)	612, 1169, 1290, 1645, 1722
BseRI	GAGGAG	4 cut(s)	542, 1761, 1810, 2507
BseSI	GKGCMC	2 cut(s)	619, 2044
BseXI	GCAGC	5 cut(s)	859, 904, 1365, 1894, 2050
BsgI	GTGCAG	1 cut(s)	735
BshFI	GGCC	5 cut(s)	1050, 1593, 1756, 1817, 2055
BshNI	GGYRCC	1 cut(s)	2390
BsiHKAI	GWGCWC	3 cut(s)	51, 1729, 2044
BsiHKCI	CYCGRG	1 cut(s)	1545
BslFI	GGGAC	1 cut(s)	2465
BslI	CCNNNNNNNGG	6 cut(s)	552, 818, 1031, 1827, 2078, 2255
BsmAI	GTCTC	4 cut(s)	150, 1405, 1607, 1733
BsmFI	GGGAC	1 cut(s)	2465
BsmI	GAATGC	2 cut(s)	2323, 2444
BsnI	GGCC	5 cut(s)	1050, 1593, 1756, 1817, 2055
Bso31I	GGTCTC	1 cut(s)	150
BsoBI	CYCGRG	1 cut(s)	1545
Bsp1286I	GDGCHC	5 cut(s)	51, 619, 1729, 1839, 2044
Bsp143I	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
BspACI	CCGC	3 cut(s)	541, 546, 1088
BspANI	GGCC	5 cut(s)	1050, 1593, 1756, 1817, 2055
BspCNI	CTCAG	6 cut(s)	63, 117, 1415, 1873, 1984, 2077
BspHI	TCATGA	1 cut(s)	523
BspLI	GGNNCC	5 cut(s)	2220, 2260, 2376, 2392, 2438
BspMAI	CTGCAG	5 cut(s)	727, 805, 1302, 1612, 2068
BspMI	ACCTGC	2 cut(s)	2057, 2511
BspPI	GGATC	3 cut(s)	36, 123, 280
BspQI	GCTCTTC	3 cut(s)	375, 1404, 1734
BspT107I	GGYRCC	1 cut(s)	2390
BspTI	CTTAAG	3 cut(s)	409, 1123, 1493
BspTNI	GGTCTC	1 cut(s)	150
BsrDI	GCAATG	2 cut(s)	132, 2518
BsrI	ACTGG	5 cut(s)	612, 1169, 1290, 1645, 1722
BssECI	CCNNGG	5 cut(s)	24, 709, 1104, 1826, 2418
BssMI	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
BssT1I	CCWWGG	2 cut(s)	1104, 2418
Bst2UI	CCWGG	4 cut(s)	25, 644, 711, 1092
Bst4CI	ACNGT	6 cut(s)	88, 250, 390, 1512, 1718, 1774
Bst6I	CTCTTC	4 cut(s)	375, 1404, 1734, 2581
BstAFI	CTTAAG	3 cut(s)	409, 1123, 1493
BstAPI	GCANNNNNTGC	1 cut(s)	722
BstBAI	YACGTR	1 cut(s)	221
BstC8I	GCNNGC	3 cut(s)	1383, 2053, 2379
BstDEI	CTNAG	9 cut(s)	40, 50, 125, 934, 1402, 1860, 1992, 2085, 2557
BstF5I	GGATG	7 cut(s)	945, 1180, 1258, 1321, 1630, 1926, 2354
BstH2I	RGCGCY	2 cut(s)	125, 2052
BstHHI	GCGC	4 cut(s)	124, 954, 1644, 2051
BstKTI	GATC	6 cut(s)	31, 67, 131, 288, 765, 1762
BstMAI	GTCTC	4 cut(s)	150, 1405, 1607, 1733
BstMBI	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
BstMWI	GCNNNNNNNGC	9 cut(s)	540, 554, 623, 722, 951, 1498, 1814, 2048, 2252
BstNI	CCWGG	4 cut(s)	25, 644, 711, 1092
BstSCI	CCNGG	4 cut(s)	23, 642, 709, 1090
BstSFI	CTRYAG	6 cut(s)	266, 723, 801, 1298, 1608, 2064
BstSLI	GKGCMC	2 cut(s)	619, 2044
BstSNI	TACGTA	1 cut(s)	221
BstV1I	GCAGC	5 cut(s)	859, 904, 1365, 1894, 2050
BstV2I	GAAGAC	1 cut(s)	2414
BstX2I	RGATCY	2 cut(s)	128, 1759
BstYI	RGATCY	2 cut(s)	128, 1759
BsuRI	GGCC	5 cut(s)	1050, 1593, 1756, 1817, 2055
BtgZI	GCGATG	1 cut(s)	804
BtrI	CACGTC	2 cut(s)	924, 1135
BtsCI	GGATG	7 cut(s)	945, 1180, 1258, 1321, 1630, 1926, 2354
BtsI	GCAGTG	3 cut(s)	671, 863, 2469
BtsIMutI	CAGTG	7 cut(s)	619, 671, 863, 1176, 1729, 2082, 2469
BveI	ACCTGC	2 cut(s)	2057, 2511
Cac8I	GCNNGC	3 cut(s)	1383, 2053, 2379
CaiI	CAGNNNCTG	2 cut(s)	722, 1031
CciI	TCATGA	1 cut(s)	523
CfoI	GCGC	4 cut(s)	124, 954, 1644, 2051
Cfr13I	GGNCC	4 cut(s)	538, 2069, 2436, 2585
CseI	GACGC	1 cut(s)	571
CsiI	ACCWGGT	1 cut(s)	642
Csp6I	GTAC	9 cut(s)	218, 931, 1283, 1452, 1550, 1719, 1946, 2175, 2283
CviAII	CATG	8 cut(s)	164, 190, 205, 524, 535, 578, 623, 1076
CviQI	GTAC	9 cut(s)	218, 931, 1283, 1452, 1550, 1719, 1946, 2175, 2283
DdeI	CTNAG	9 cut(s)	40, 50, 125, 934, 1402, 1860, 1992, 2085, 2557
DpnI	GATC	6 cut(s)	30, 66, 130, 287, 764, 1761
DpnII	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
DraIII	CACNNNGTG	1 cut(s)	1171
EaeI	YGGCCR	3 cut(s)	1048, 1591, 1754
Eam1104I	CTCTTC	4 cut(s)	375, 1404, 1734, 2581
EarI	CTCTTC	4 cut(s)	375, 1404, 1734, 2581
Ecl136II	GAGCTC	1 cut(s)	49
Eco105I	TACGTA	1 cut(s)	221
Eco130I	CCWWGG	2 cut(s)	1104, 2418
Eco147I	AGGCCT	1 cut(s)	1817
Eco24I	GRGCYC	2 cut(s)	51, 1839
Eco31I	GGTCTC	1 cut(s)	150
Eco47I	GGWCC	4 cut(s)	538, 2069, 2436, 2585
Eco47III	AGCGCT	1 cut(s)	2050
Eco53kI	GAGCTC	1 cut(s)	49
Eco57I	CTGAAG	4 cut(s)	242, 1410, 1896, 2220
Eco88I	CYCGRG	1 cut(s)	1545
EcoICRI	GAGCTC	1 cut(s)	49
EcoO109I	RGGNCCY	1 cut(s)	2585
EcoRI	GAATTC	2 cut(s)	807, 1341
EcoRII	CCWGG	4 cut(s)	23, 642, 709, 1090
EcoT14I	CCWWGG	2 cut(s)	1104, 2418
EcoT22I	ATGCAT	2 cut(s)	685, 1576
EcoT38I	GRGCYC	2 cut(s)	51, 1839
ErhI	CCWWGG	2 cut(s)	1104, 2418
FaeI	CATG	8 cut(s)	167, 193, 208, 527, 538, 581, 626, 1079
FalI	AAGNNNNNCTT	2 cut(s)	1401, 1433
FaqI	GGGAC	1 cut(s)	2465
FatI	CATG	8 cut(s)	163, 189, 204, 523, 534, 577, 622, 1075
FbaI	TGATCA	1 cut(s)	64
FblI	GTMKAC	1 cut(s)	1483
Fnu4HI	GCNGC	5 cut(s)	848, 918, 1379, 1908, 2064
FokI	GGATG	7 cut(s)	932, 1187, 1265, 1328, 1637, 1933, 2341
FriOI	GRGCYC	2 cut(s)	51, 1839
Fsp4HI	GCNGC	5 cut(s)	848, 918, 1379, 1908, 2064
FspBI	CTAG	7 cut(s)	431, 1328, 1539, 1743, 2057, 2159, 2441
FspI	TGCGCA	1 cut(s)	953
GlaI	GCGC	4 cut(s)	123, 953, 1643, 2050
GluI	GCNGC	5 cut(s)	848, 918, 1379, 1908, 2064
GsuI	CTGGAG	2 cut(s)	1008, 1053
HaeII	RGCGCY	2 cut(s)	125, 2052
HaeIII	GGCC	5 cut(s)	1050, 1593, 1756, 1817, 2055
HgaI	GACGC	1 cut(s)	571
HhaI	GCGC	4 cut(s)	124, 954, 1644, 2051
Hin1II	CATG	8 cut(s)	167, 193, 208, 527, 538, 581, 626, 1079
Hin6I	GCGC	4 cut(s)	122, 952, 1642, 2049
HinP1I	GCGC	4 cut(s)	122, 952, 1642, 2049
HincII	GTYRAC	3 cut(s)	598, 1085, 1132
HindII	GTYRAC	3 cut(s)	598, 1085, 1132
HindIII	AAGCTT	4 cut(s)	1383, 1490, 2075, 2554
HphI	GGTGA	4 cut(s)	601, 1157, 1406, 1925
Hpy166II	GTNNAC	7 cut(s)	100, 598, 1085, 1132, 1283, 1484, 2042
Hpy188I	TCNGA	8 cut(s)	246, 659, 694, 1429, 1669, 1830, 1863, 1876
Hpy8I	GTNNAC	7 cut(s)	100, 598, 1085, 1132, 1283, 1484, 2042
HpyAV	CCTTC	6 cut(s)	223, 415, 654, 885, 893, 1103
HpyCH4III	ACNGT	6 cut(s)	88, 250, 390, 1512, 1718, 1774
HpyCH4IV	ACGT	6 cut(s)	149, 220, 923, 1134, 1432, 2143
HpyF10VI	GCNNNNNNNGC	9 cut(s)	540, 554, 623, 722, 951, 1498, 1814, 2048, 2252
HpyF3I	CTNAG	9 cut(s)	40, 50, 125, 934, 1402, 1860, 1992, 2085, 2557
HpySE526I	ACGT	6 cut(s)	149, 220, 923, 1134, 1432, 2143
Hsp92II	CATG	8 cut(s)	167, 193, 208, 527, 538, 581, 626, 1079
HspAI	GCGC	4 cut(s)	122, 952, 1642, 2049
Ksp22I	TGATCA	1 cut(s)	64
Kzo9I	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
LguI	GCTCTTC	3 cut(s)	375, 1404, 1734
LmnI	GCTCC	4 cut(s)	1034, 1498, 2218, 2374
Lsp1109I	GCAGC	5 cut(s)	859, 904, 1365, 1894, 2050
LweI	GCATC	7 cut(s)	678, 954, 1003, 1321, 1561, 2050, 2092
MabI	ACCWGGT	1 cut(s)	642
MaeI	CTAG	7 cut(s)	431, 1328, 1539, 1743, 2057, 2159, 2441
MaeII	ACGT	6 cut(s)	149, 220, 923, 1134, 1432, 2143
MaeIII	GTNAC	6 cut(s)	252, 607, 1177, 1647, 1768, 2334
MalI	GATC	6 cut(s)	30, 66, 130, 287, 764, 1761
MboI	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
MflI	RGATCY	2 cut(s)	128, 1759
MhlI	GDGCHC	5 cut(s)	51, 619, 1729, 1839, 2044
MlsI	TGGCCA	2 cut(s)	1050, 1756
MluNI	TGGCCA	2 cut(s)	1050, 1756
MlyI	GAGTC	7 cut(s)	161, 689, 728, 1037, 1154, 1673, 1758
MmeI	TCCRAC	2 cut(s)	562, 1096
Mox20I	TGGCCA	2 cut(s)	1050, 1756
Mph1103I	ATGCAT	2 cut(s)	685, 1576
MscI	TGGCCA	2 cut(s)	1050, 1756
MslI	CAYNNNNRTG	1 cut(s)	2182
Msp20I	TGGCCA	2 cut(s)	1050, 1756
MspCI	CTTAAG	3 cut(s)	409, 1123, 1493
MspR9I	CCNGG	4 cut(s)	25, 644, 711, 1092
Mva1269I	GAATGC	2 cut(s)	2323, 2444
MvaI	CCWGG	4 cut(s)	25, 644, 711, 1092
MwoI	GCNNNNNNNGC	9 cut(s)	540, 554, 623, 722, 951, 1498, 1814, 2048, 2252
NdeII	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
NlaIII	CATG	8 cut(s)	167, 193, 208, 527, 538, 581, 626, 1079
NlaIV	GGNNCC	5 cut(s)	2220, 2260, 2376, 2392, 2438
NmuCI	GTSAC	2 cut(s)	252, 607
NsbI	TGCGCA	1 cut(s)	953
NsiI	ATGCAT	2 cut(s)	685, 1576
PaeR7I	CTCGAG	1 cut(s)	1545
PagI	TCATGA	1 cut(s)	523
PceI	AGGCCT	1 cut(s)	1817
PciSI	GCTCTTC	3 cut(s)	375, 1404, 1734
PcsI	WCGNNNNNNNCGW	1 cut(s)	1140
PctI	GAATGC	2 cut(s)	2323, 2444
PfeI	GAWTC	9 cut(s)	1119, 1202, 1319, 1703, 1895, 2289, 2346, 2459, 2564
PflFI	GACNNNGTC	1 cut(s)	250
PflMI	CCANNNNNTGG	2 cut(s)	1031, 2078
PkrI	GCNGC	5 cut(s)	849, 919, 1380, 1909, 2065
PleI	GAGTC	7 cut(s)	161, 689, 727, 1036, 1153, 1672, 1757
PpsI	GAGTC	7 cut(s)	161, 689, 727, 1036, 1153, 1672, 1757
Ppu21I	YACGTR	1 cut(s)	221
PpuMI	RGGWCCY	1 cut(s)	2585
PshBI	ATTAAT	1 cut(s)	740
PsiI	TTATAA	1 cut(s)	74
Psp124BI	GAGCTC	1 cut(s)	51
Psp5II	RGGWCCY	1 cut(s)	2585
Psp6I	CCWGG	4 cut(s)	23, 642, 709, 1090
PspGI	CCWGG	4 cut(s)	23, 642, 709, 1090
PspN4I	GGNNCC	5 cut(s)	2220, 2260, 2376, 2392, 2438
PspPI	GGNCC	4 cut(s)	538, 2069, 2436, 2585
PspPPI	RGGWCCY	1 cut(s)	2585
PspXI	VCTCGAGB	1 cut(s)	1545
PstI	CTGCAG	5 cut(s)	727, 805, 1302, 1612, 2068
PstNI	CAGNNNCTG	2 cut(s)	722, 1031
PsuI	RGATCY	2 cut(s)	128, 1759
PsyI	GACNNNGTC	1 cut(s)	250
RsaI	GTAC	9 cut(s)	219, 932, 1284, 1453, 1551, 1720, 1947, 2176, 2284
RsaNI	GTAC	9 cut(s)	218, 931, 1283, 1452, 1550, 1719, 1946, 2175, 2283
RseI	CAYNNNNRTG	1 cut(s)	2182
SacI	GAGCTC	1 cut(s)	51
SapI	GCTCTTC	3 cut(s)	375, 1404, 1734
SatI	GCNGC	5 cut(s)	848, 918, 1379, 1908, 2064
Sau3AI	GATC	6 cut(s)	28, 64, 128, 285, 762, 1759
Sau96I	GGNCC	4 cut(s)	538, 2069, 2436, 2585
SchI	GAGTC	7 cut(s)	161, 689, 728, 1037, 1154, 1673, 1758
ScrFI	CCNGG	4 cut(s)	25, 644, 711, 1092
SduI	GDGCHC	5 cut(s)	51, 619, 1729, 1839, 2044
SexAI	ACCWGGT	1 cut(s)	642
SfaNI	GCATC	7 cut(s)	678, 954, 1003, 1321, 1561, 2050, 2092
SfcI	CTRYAG	6 cut(s)	266, 723, 801, 1298, 1608, 2064
Sfr274I	CTCGAG	1 cut(s)	1545
SinI	GGWCC	4 cut(s)	538, 2069, 2436, 2585
SlaI	CTCGAG	1 cut(s)	1545
SmiMI	CAYNNNNRTG	1 cut(s)	2182
SmlI	CTYRAG	7 cut(s)	409, 1123, 1493, 1545, 1688, 1733, 2580
SmoI	CTYRAG	7 cut(s)	409, 1123, 1493, 1545, 1688, 1733, 2580
SnaBI	TACGTA	1 cut(s)	221
SseBI	AGGCCT	1 cut(s)	1817
SsiI	CCGC	3 cut(s)	541, 546, 1088
SspMI	CTAG	7 cut(s)	431, 1328, 1539, 1743, 2057, 2159, 2441
SstI	GAGCTC	1 cut(s)	51
StuI	AGGCCT	1 cut(s)	1817
StyD4I	CCNGG	4 cut(s)	23, 642, 709, 1090
StyI	CCWWGG	2 cut(s)	1104, 2418
TaaI	ACNGT	6 cut(s)	88, 250, 390, 1512, 1718, 1774
TaiI	ACGT	6 cut(s)	152, 223, 926, 1137, 1435, 2146
TaqI	TCGA	6 cut(s)	765, 897, 1442, 1546, 2405, 2590
TatI	WGTACW	4 cut(s)	930, 1282, 1549, 1945
TfiI	GAWTC	9 cut(s)	1119, 1202, 1319, 1703, 1895, 2289, 2346, 2459, 2564
TscAI	CASTG	7 cut(s)	619, 671, 870, 1176, 1729, 2089, 2476
TseFI	GTSAC	2 cut(s)	252, 607
TseI	GCWGC	5 cut(s)	847, 917, 1378, 1907, 2063
Tsp45I	GTSAC	2 cut(s)	252, 607
TspGWI	ACGGA	2 cut(s)	996, 2339
TspRI	CASTG	7 cut(s)	619, 671, 870, 1176, 1729, 2089, 2476
Tth111I	GACNNNGTC	1 cut(s)	250
Van91I	CCANNNNNTGG	2 cut(s)	1031, 2078
Vha464I	CTTAAG	3 cut(s)	409, 1123, 1493
VneI	GTGCAC	1 cut(s)	2040
VpaK11BI	GGWCC	4 cut(s)	538, 2069, 2436, 2585
VspI	ATTAAT	1 cut(s)	740
XapI	RAATTY	6 cut(s)	182, 650, 807, 1341, 1965, 2032
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2269
XhoI	CTCGAG	1 cut(s)	1545
XmiI	GTMKAC	1 cut(s)	1483
XspI	CTAG	7 cut(s)	431, 1328, 1539, 1743, 2057, 2159, 2441
Zsp2I	ATGCAT	2 cut(s)	685, 1576

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.