MD13G1245700.v1.1

Protein of unknown function (DUF630)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005108
Physical Location & Seq
Reverse (-)
25762018 .. 25763579
1562 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1245700.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGTACCACAAGAAAGGAGTATGGCAGAGTGGACTTGAGATTGAGCTCGAAGCACAAATTCCATCACATAGTAGTGTGAGTATAGAGGTTTCGCAATCTCAGACTGCTAGACAAGTTAAAACTAATAGCCAAGAAAGAGCTGTTGAGCTCCGGGAAGCTAAGGAAGAAACACCAGGCTTTACTGTATACATGAACAGAAGGCCAACGGGTATGGCAGAGATTATCAAGGAATTAGATACTCAATTCACAATTGTTTGCAATGCCGCCAATGAGGTATCAACGTTGTTAGAGGCTAGCAGAGAGCAGTACTCGTCATCCTCCAATGAACTCTCAGCCATGAAAATGTTGAACCCAGTAGCATTATTTTGCTCAGCATCGTCCAGATTTTTAATGAATTCTTCGTGCACTAAAGATGAAGGTTATGAAAGCAGTAGTGATGTCTCAGAGGATCCCTGCATGTTTTCTGGTAGCCACCAATCAACATTGGACAGATTATATGCTTGGGAGAAGAAACTCTACGAGGAAGTCAAGTCCGGAGAAAAGGTACGGATTGCATATGAAAAGAAACTGATGCAACTACGGAACCAAGATGTAAAAGGCGATGATCATTCCGCTGTAGAAAAAACGAGGGTAGCAATTAGAGATATGCATACTCGGATGAAGGTTTCAATACACACGGTTGAAGCTATTTCGAAGAGGATTGAAACCATGAGGGATGAAGAATTGCAACCTCAACTCTCGGAATTGGTACAAGGGTTAGCAAGGATGTGGAAAGTAATGGCAAAGTGTCATCGGTCGCAGAAACGAAGCCTAGATGAAGCCAAGCTTTTACTAGCAGGCACGCCGTCAAAATTGGAAGCTAAACGACAGTCTTCCATTTCAGCCGCTGATCCAAACCGGCTAGCACGTTCAGCAGCCAATCTTGAGAAAGATCTGAGGAACTGGAGAGCCTTGTTCCAGTCATGGATCACTTCTCAACGATCTTATGTGCAAGCAATAACAGGTTGGCTCCTCTGTTGCATGAGGGCTGGTCCCGACACATCAAATTTTCCGTTGTCTGGCTCCAGTGGGGCGCTTCCAATATATGGACTATGCATCCAATGGTCAAGGTTCCTGGATGCCATACGTGAGACACCAGTGCTTGATGGACTAGATTTTTTTGCAGCTGGTATGGGGTCCCTCTACGCGCAACAGCTTAGAGAGGATTCGCGCCGAGTTAGGAGATGGTGGCAGAGGTCACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

415

Amino Acids

47.0

Weight (kDa)

8.79

Isoelectric Point (pI)

59.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 71 - 391 3.5e-100 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1085
AccI GTMKAC 1 cut(s) 186
AccII CGCG 2 cut(s) 1187, 1210
AccIII TCCGGA 1 cut(s) 533
AciI CCGC 3 cut(s) 264, 612, 885
AclI AACGTT 1 cut(s) 281
AclWI GGATC 4 cut(s) 443, 456, 884, 974
AcsI RAATTY 3 cut(s) 57, 394, 1045
AfaI GTAC 4 cut(s) 5, 308, 546, 750
AfiI CCNNNNNNNGG 2 cut(s) 1058, 1085
AgsI TTSAA 4 cut(s) 349, 669, 683, 704
AjnI CCWGG 2 cut(s) 172, 1113
AluBI AGCT 9 cut(s) 46, 140, 148, 158, 686, 826, 860, 1166, 1195
AluI AGCT 9 cut(s) 46, 140, 148, 158, 686, 826, 860, 1166, 1195
Alw21I GWGCWC 3 cut(s) 48, 150, 407
Alw26I GTCTC 2 cut(s) 445, 1124
Alw44I GTGCAC 1 cut(s) 403
AlwI GGATC 4 cut(s) 443, 456, 884, 974
AlwNI CAGNNNCTG 1 cut(s) 887
Aor13HI TCCGGA 1 cut(s) 533
AoxI GGCC 1 cut(s) 200
ApaLI GTGCAC 1 cut(s) 403
ApeKI GCWGC 2 cut(s) 914, 1163
ApoI RAATTY 3 cut(s) 57, 394, 1045
AspLEI GCGC 3 cut(s) 1075, 1189, 1212
AspS9I GGNCC 2 cut(s) 1031, 1176
AsuC2I CCSGG 1 cut(s) 152
AsuII TTCGAA 1 cut(s) 692
AsuNHI GCTAGC 2 cut(s) 293, 901
AvaII GGWCC 2 cut(s) 1031, 1176
BaeGI GKGCMC 1 cut(s) 407
BaeI ACNNNNGTAYC 2 cut(s) 740, 773
BamHI GGATCC 1 cut(s) 448
BanII GRGCYC 2 cut(s) 48, 150
BarI GAAGNNNNNNTAC 2 cut(s) 500, 532
BbsI GAAGAC 1 cut(s) 864
Bbv12I GWGCWC 3 cut(s) 48, 150, 407
BbvI GCAGC 2 cut(s) 926, 1175
BccI CCATC 3 cut(s) 70, 1139, 1218
BceAI ACGGC 1 cut(s) 829
BciT130I CCWGG 2 cut(s) 174, 1115
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 2 cut(s) 445, 1124
BfaI CTAG 6 cut(s) 108, 294, 812, 833, 902, 1151
BfmI CTRYAG 1 cut(s) 615
BfoI RGCGCY 1 cut(s) 1076
BglII AGATCT 1 cut(s) 931
BisI GCNGC 4 cut(s) 264, 885, 915, 1164
BlpI GCTNAGC 1 cut(s) 370
BlsI GCNGC 4 cut(s) 265, 886, 916, 1165
BmcAI AGTACT 1 cut(s) 308
Bme1390I CCNGG 3 cut(s) 152, 174, 1115
Bme18I GGWCC 2 cut(s) 1031, 1176
BmgT120I GGNCC 2 cut(s) 1031, 1176
BmiI GGNNCC 8 cut(s) 450, 584, 1010, 1033, 1063, 1112, 1177, 1178
BmrFI CCNGG 3 cut(s) 152, 174, 1115
BmrI ACTGGG 1 cut(s) 347
BmsI GCATC 4 cut(s) 383, 561, 1104, 1108
BmtI GCTAGC 2 cut(s) 297, 905
BmuI ACTGGG 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 864
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 2 cut(s) 964, 1048
Bpu10I CCTNAGC 1 cut(s) 159
Bpu1102I GCTNAGC 1 cut(s) 370
Bpu14I TTCGAA 1 cut(s) 692
BpuEI CTTGAG 2 cut(s) 56, 944
BpuMI CCSGG 1 cut(s) 152
BsaAI YACGTR 1 cut(s) 1127
BsaWI WCCGGW 1 cut(s) 533
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 1058, 1085
Bse118I RCCGGY 1 cut(s) 897
Bse1I ACTGG 5 cut(s) 353, 947, 958, 1065, 1136
Bse3DI GCAATG 1 cut(s) 265
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 2 cut(s) 174, 1115
BseGI GGATG 6 cut(s) 314, 663, 721, 771, 1095, 1123
BseLI CCNNNNNNNGG 2 cut(s) 1058, 1085
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 5 cut(s) 113, 345, 384, 456, 926
BseNI ACTGG 5 cut(s) 353, 947, 958, 1065, 1136
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 1 cut(s) 407
BseXI GCAGC 2 cut(s) 926, 1175
Bsh1236I CGCG 2 cut(s) 1187, 1210
Bsh1285I CGRYCG 1 cut(s) 797
BshFI GGCC 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 797
BsiHKAI GWGCWC 3 cut(s) 48, 150, 407
BsiSI CCGG 3 cut(s) 151, 534, 898
BslFI GGGAC 2 cut(s) 1017, 1162
BslI CCNNNNNNNGG 2 cut(s) 1058, 1085
BsmAI GTCTC 2 cut(s) 445, 1124
BsmFI GGGAC 2 cut(s) 1017, 1162
BsnI GGCC 1 cut(s) 202
Bsp119I TTCGAA 1 cut(s) 692
Bsp1286I GDGCHC 3 cut(s) 48, 150, 407
Bsp13I TCCGGA 1 cut(s) 533
Bsp143I GATC 6 cut(s) 448, 604, 889, 931, 966, 980
Bsp1720I GCTNAGC 1 cut(s) 370
BspACI CCGC 3 cut(s) 264, 612, 885
BspANI GGCC 1 cut(s) 202
BspCNI CTCAG 5 cut(s) 112, 344, 383, 455, 927
BspEI TCCGGA 1 cut(s) 533
BspFNI CGCG 2 cut(s) 1187, 1210
BspLI GGNNCC 8 cut(s) 450, 584, 1010, 1033, 1063, 1112, 1177, 1178
BspOI GCTAGC 2 cut(s) 297, 905
BspPI GGATC 4 cut(s) 443, 456, 884, 974
BspT104I TTCGAA 1 cut(s) 692
BsrDI GCAATG 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 897
BsrI ACTGG 5 cut(s) 353, 947, 958, 1065, 1136
BssAI RCCGGY 1 cut(s) 897
BssMI GATC 6 cut(s) 448, 604, 889, 931, 966, 980
BssNAI GTATAC 1 cut(s) 187
Bst1107I GTATAC 1 cut(s) 187
Bst2UI CCWGG 2 cut(s) 174, 1115
Bst4CI ACNGT 4 cut(s) 184, 679, 870, 1242
Bst6I CTCTTC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 1127
BstBI TTCGAA 1 cut(s) 692
BstC8I GCNNGC 5 cut(s) 295, 838, 842, 903, 993
BstDEI CTNAG 7 cut(s) 99, 159, 331, 370, 442, 935, 1196
BstF5I GGATG 6 cut(s) 314, 663, 721, 771, 1095, 1123
BstFNI CGCG 2 cut(s) 1187, 1210
BstH2I RGCGCY 1 cut(s) 1076
BstHHI GCGC 3 cut(s) 1075, 1189, 1212
BstKTI GATC 6 cut(s) 451, 607, 892, 934, 969, 983
BstMAI GTCTC 2 cut(s) 445, 1124
BstMBI GATC 6 cut(s) 448, 604, 889, 931, 966, 980
BstMCI CGRYCG 1 cut(s) 797
BstMWI GCNNNNNNNGC 1 cut(s) 911
BstNI CCWGG 2 cut(s) 174, 1115
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 3 cut(s) 150, 172, 1113
BstSFI CTRYAG 1 cut(s) 615
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 2 cut(s) 1187, 1210
BstV1I GCAGC 2 cut(s) 926, 1175
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 2 cut(s) 448, 931
BstYI RGATCY 2 cut(s) 448, 931
BstZ17I GTATAC 1 cut(s) 187
BsuRI GGCC 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 615
BtsCI GGATG 6 cut(s) 314, 663, 721, 771, 1095, 1123
BtsIMutI CAGTG 2 cut(s) 1072, 1143
Cac8I GCNNGC 5 cut(s) 295, 838, 842, 903, 993
CaiI CAGNNNCTG 1 cut(s) 887
CfoI GCGC 3 cut(s) 1075, 1189, 1212
Cfr10I RCCGGY 1 cut(s) 897
Cfr13I GGNCC 2 cut(s) 1031, 1176
Csp6I GTAC 4 cut(s) 4, 307, 545, 749
CviAII CATG 6 cut(s) 190, 337, 457, 709, 963, 1021
CviQI GTAC 4 cut(s) 4, 307, 545, 749
DdeI CTNAG 7 cut(s) 99, 159, 331, 370, 442, 935, 1196
DpnI GATC 6 cut(s) 450, 606, 891, 933, 968, 982
DpnII GATC 6 cut(s) 448, 604, 889, 931, 966, 980
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
Ecl136II GAGCTC 2 cut(s) 46, 148
Eco24I GRGCYC 2 cut(s) 48, 150
Eco47I GGWCC 2 cut(s) 1031, 1176
Eco53kI GAGCTC 2 cut(s) 46, 148
EcoICRI GAGCTC 2 cut(s) 46, 148
EcoO109I RGGNCCY 1 cut(s) 1176
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 172, 1113
EcoT22I ATGCAT 2 cut(s) 651, 1097
EcoT38I GRGCYC 2 cut(s) 48, 150
FaeI CATG 6 cut(s) 193, 340, 460, 712, 966, 1024
FalI AAGNNNNNCTT 2 cut(s) 810, 842
FaqI GGGAC 2 cut(s) 1017, 1162
FatI CATG 6 cut(s) 189, 336, 456, 708, 962, 1020
FauNDI CATATG 1 cut(s) 556
FbaI TGATCA 1 cut(s) 604
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 264, 885, 915, 1164
FokI GGATG 6 cut(s) 301, 670, 728, 778, 1082, 1130
FriOI GRGCYC 2 cut(s) 48, 150
Fsp4HI GCNGC 4 cut(s) 264, 885, 915, 1164
FspBI CTAG 6 cut(s) 108, 294, 812, 833, 902, 1151
GlaI GCGC 3 cut(s) 1074, 1188, 1211
GluI GCNGC 4 cut(s) 264, 885, 915, 1164
GsuI CTGGAG 2 cut(s) 964, 1048
HaeII RGCGCY 1 cut(s) 1076
HaeIII GGCC 1 cut(s) 202
HapII CCGG 3 cut(s) 151, 534, 898
HhaI GCGC 3 cut(s) 1075, 1189, 1212
Hin1II CATG 6 cut(s) 193, 340, 460, 712, 966, 1024
Hin6I GCGC 3 cut(s) 1073, 1187, 1210
HinP1I GCGC 3 cut(s) 1073, 1187, 1210
HindIII AAGCTT 1 cut(s) 824
HinfI GANTC 1 cut(s) 1205
HpaII CCGG 3 cut(s) 151, 534, 898
Hpy166II GTNNAC 3 cut(s) 32, 187, 405
Hpy188I TCNGA 5 cut(s) 102, 445, 657, 742, 936
Hpy188III TCNNGA 4 cut(s) 381, 534, 923, 1034
Hpy8I GTNNAC 3 cut(s) 32, 187, 405
HpyAV CCTTC 3 cut(s) 192, 410, 655
HpyCH4III ACNGT 4 cut(s) 184, 679, 870, 1242
HpyCH4IV ACGT 3 cut(s) 281, 907, 1126
HpyF10VI GCNNNNNNNGC 1 cut(s) 911
HpyF3I CTNAG 7 cut(s) 99, 159, 331, 370, 442, 935, 1196
HpySE526I ACGT 3 cut(s) 281, 907, 1126
Hsp92II CATG 6 cut(s) 193, 340, 460, 712, 966, 1024
HspAI GCGC 3 cut(s) 1073, 1187, 1210
KflI GGGWCCC 1 cut(s) 1176
Kpn2I TCCGGA 1 cut(s) 533
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 6 cut(s) 448, 604, 889, 931, 966, 980
LmnI GCTCC 3 cut(s) 153, 1014, 1067
Lsp1109I GCAGC 2 cut(s) 926, 1175
LweI GCATC 4 cut(s) 383, 561, 1104, 1108
MaeI CTAG 6 cut(s) 108, 294, 812, 833, 902, 1151
MaeII ACGT 3 cut(s) 281, 907, 1126
MaeIII GTNAC 1 cut(s) 1236
MalI GATC 6 cut(s) 450, 606, 891, 933, 968, 982
MboI GATC 6 cut(s) 448, 604, 889, 931, 966, 980
MboII GAAGA 6 cut(s) 176, 390, 520, 706, 731, 864
MfeI CAATTG 1 cut(s) 249
MflI RGATCY 2 cut(s) 448, 931
MhlI GDGCHC 3 cut(s) 48, 150, 407
Mph1103I ATGCAT 2 cut(s) 651, 1097
MroI TCCGGA 1 cut(s) 533
MseI TTAA 2 cut(s) 117, 389
MslI CAYNNNNRTG 2 cut(s) 72, 341
MspA1I CMGCKG 3 cut(s) 614, 887, 1166
MspI CCGG 3 cut(s) 151, 534, 898
MspR9I CCNGG 3 cut(s) 152, 174, 1115
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 2 cut(s) 174, 1115
MvnI CGCG 2 cut(s) 1187, 1210
MwoI GCNNNNNNNGC 1 cut(s) 911
NciI CCSGG 1 cut(s) 152
NdeI CATATG 1 cut(s) 556
NdeII GATC 6 cut(s) 448, 604, 889, 931, 966, 980
NheI GCTAGC 2 cut(s) 293, 901
NlaIII CATG 6 cut(s) 193, 340, 460, 712, 966, 1024
NlaIV GGNNCC 8 cut(s) 450, 584, 1010, 1033, 1063, 1112, 1177, 1178
NmeAIII GCCGAG 1 cut(s) 1238
NmuCI GTSAC 1 cut(s) 1236
NsiI ATGCAT 2 cut(s) 651, 1097
NspI RCATGY 1 cut(s) 460
NspV TTCGAA 1 cut(s) 692
PfeI GAWTC 1 cut(s) 1205
PflMI CCANNNNNTGG 1 cut(s) 1085
PfoI TCCNGGA 2 cut(s) 150, 1113
PkrI GCNGC 4 cut(s) 265, 886, 916, 1165
Ppu21I YACGTR 1 cut(s) 1127
PpuMI RGGWCCY 1 cut(s) 1176
Psp124BI GAGCTC 2 cut(s) 48, 150
Psp1406I AACGTT 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 1176
Psp6I CCWGG 2 cut(s) 172, 1113
PspGI CCWGG 2 cut(s) 172, 1113
PspN4I GGNNCC 8 cut(s) 450, 584, 1010, 1033, 1063, 1112, 1177, 1178
PspPI GGNCC 2 cut(s) 1031, 1176
PspPPI RGGWCCY 1 cut(s) 1176
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 2 cut(s) 448, 931
PvuII CAGCTG 1 cut(s) 1166
RsaI GTAC 4 cut(s) 5, 308, 546, 750
RsaNI GTAC 4 cut(s) 4, 307, 545, 749
RseI CAYNNNNRTG 2 cut(s) 72, 341
SacI GAGCTC 2 cut(s) 48, 150
SaqAI TTAA 2 cut(s) 117, 389
SatI GCNGC 4 cut(s) 264, 885, 915, 1164
Sau3AI GATC 6 cut(s) 448, 604, 889, 931, 966, 980
Sau96I GGNCC 2 cut(s) 1031, 1176
ScaI AGTACT 1 cut(s) 308
ScrFI CCNGG 3 cut(s) 152, 174, 1115
SduI GDGCHC 3 cut(s) 48, 150, 407
SfaNI GCATC 4 cut(s) 383, 561, 1104, 1108
SfcI CTRYAG 1 cut(s) 615
SfuI TTCGAA 1 cut(s) 692
SinI GGWCC 2 cut(s) 1031, 1176
SmiMI CAYNNNNRTG 2 cut(s) 72, 341
SmlI CTYRAG 2 cut(s) 35, 923
SmoI CTYRAG 2 cut(s) 35, 923
SsiI CCGC 3 cut(s) 264, 612, 885
SspMI CTAG 6 cut(s) 108, 294, 812, 833, 902, 1151
SstI GAGCTC 2 cut(s) 48, 150
StyD4I CCNGG 3 cut(s) 150, 172, 1113
TaaI ACNGT 4 cut(s) 184, 679, 870, 1242
TaiI ACGT 3 cut(s) 284, 910, 1129
TaqI TCGA 2 cut(s) 48, 692
TaqII GACCGA 1 cut(s) 783
TatI WGTACW 1 cut(s) 306
TauI GCSGC 2 cut(s) 266, 887
TfiI GAWTC 1 cut(s) 1205
Tru1I TTAA 2 cut(s) 117, 389
Tru9I TTAA 2 cut(s) 117, 389
TscAI CASTG 2 cut(s) 1072, 1143
TseFI GTSAC 1 cut(s) 1236
TseI GCWGC 2 cut(s) 914, 1163
Tsp45I GTSAC 1 cut(s) 1236
TspGWI ACGGA 3 cut(s) 562, 595, 1041
TspRI CASTG 2 cut(s) 1072, 1143
Van91I CCANNNNNTGG 1 cut(s) 1085
VneI GTGCAC 1 cut(s) 403
VpaK11BI GGWCC 2 cut(s) 1031, 1176
XapI RAATTY 3 cut(s) 57, 394, 1045
XceI RCATGY 1 cut(s) 460
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 6 cut(s) 108, 294, 812, 833, 902, 1151
ZrmI AGTACT 1 cut(s) 308
Zsp2I ATGCAT 2 cut(s) 651, 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.