Rroxscaffold_5G00345130

Protein of unknown function (DUF630)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005108
Physical Location & Seq
Forward (+)
15420887 .. 15426022
5136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345130.1

Sequence Viewer

Length: 1734 bp
ATGGGATTTTTCTGGAACCCATTTTCATCATTAGATTACTATGGTTATCCCACTCCATCTAGTATTGATCAGACAGTCATGGATGATGAGAATAGAGGACTAAGGCAGGTCAGAGAAGAAGAAGGGATCCCTGACTTGGAAGAAATTGAAACTGAGCAAGAGGATTGCTATGACGAAGCAAATGTAAGACAAGAAAGGGATAAAATGGATCTAAACTACAACAGAGAAGAAGTTATTGTTGAAGACGTTGATGATGAGGAGGATGAGGATGAGGAGACAGTCAGCGGAAACGAAACTGAGCATGAAGCAGAAATGCCCTCCCATGATCATGTTACTATAGAGGTGCGAGCTCAGACTGCAAGACAAGTTGAAACTAGTAATCAAGGAACGGCAGTTGTTGATCGAGAGGCCAAGGAAGAAACACCAGGATTTACTGTTTATGTAAACCGAAGGCCAACGAGTATGGCAGAAGTTATCAAGGATTTAGAAGCTCAGTTTGTGATCGTTTGCAATGCAGCCAATCAGGTCTCGGCATTGTTAGAGGCCAACAGAGAGCAGTACTCATCAACCTCCAATGAACTCTCAGCCATGAAAATGCTGAACCCAGTAGCTTTATTTCGCTCAGCATCCTCGTCTCGCTCAGCCTCATCCAGATTTTTAGTGACTTCTTCCTGCTCCAAAGATGAAGGTTATGAAAGCAGCAGTGACTTCTCAGAGGAGCCCTGCATGTTTTCAGGTAGTCACCAATCAACATTAGACAGATTGTATGCTTGGGAGAAGAAACTCTATGAGGAAGTCAAGTCTGGAGAAAAGGTTCGGATGACATATGACAAGAAATTAAAGCAACTCCGGAACCAAGATGTAAAAGGTGATGACCAATCTGCTCTAGAAAAAACAAGGGCATCTATCAGAGATCTGCATACGCAGATGAAGGTTTCGATACACTCAGTTGAAGCTATTTCAAAGAGAATTGAAACCTTAAGGGATGAAGAACTGCAGCCTCAACTTTCAGAATTGGTGCAAGGGTTAGCAAGGATGTGGAAAGTAATGGCAGAGTGTCATCAGACACAGAAACGAAGCCTCGATGAAGCCAAGCTTTTACTAGCAGGCACACCTTCAAAACTGGAAGCTAAAAGGCATTCTTCTTCCAGTTCAGTGACTGATCCAAACCGGCTAGCACGTTCTGCAGCCAATCTTGAGACCGAACTGAGGAATTGGAGAGCCTATTTCGAGTCATGGATCACTTCTCAGCGATCCTACGTACACGCATTAGCAGGATGGCTACTCCGTTGCATGAGGGCTGATCCAGATACATCAAAGTTGCCATTCTCTCCTCGTCGCTCTAGCGGTGCTCCTCCAATATTTGGAATATGCATCCAATGGTCAAGGTTTCTAGATGCCATACGTGAGACCCCAGTGCTAGATGGACTAGATTTTTTCGCAGCTGGTATGGGATCTCTCTATGCACAGCAGGTTAAAGAGGATTTACGTAGAGCTCGGATAGGTTCAAAGAGGTTCGAGTCAACTGAGGAATTCAGTGGGAATATGGAACTGGTGGAAGTTGGTCAAGTTGAACAAGTAATGACTGCAGATAAAATGGCTGAAGTTGCCATAAGGGTACTTTGTGCTGGAATGTCAGTTACTATGAGCTCACTGACAGAGTTTTCTATTGCTTCTGCTGAGGGGTATACTGATCTTGTGAATCAATGGGACGATGCCAAGACTAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

577

Amino Acids

65.31

Weight (kDa)

4.8

Isoelectric Point (pI)

53.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 155 - 484 4.5e-107 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 97, 1462
AccB7I CCANNNNNTGG 1 cut(s) 1364
AccI GTMKAC 1 cut(s) 1688
AccIII TCCGGA 1 cut(s) 849
AciI CCGC 2 cut(s) 285, 1347
AclWI GGATC 8 cut(s) 121, 134, 216, 1157, 1247, 1248, 1298, 1462
AcsI RAATTY 1 cut(s) 1532
AcuI CTGAAG 1 cut(s) 1623
AfaI GTAC 3 cut(s) 560, 1263, 1620
AfiI CCNNNNNNNGG 3 cut(s) 136, 1209, 1364
AflII CTTAAG 1 cut(s) 979
AgsI TTSAA 9 cut(s) 149, 242, 371, 953, 963, 974, 1119, 1509, 1574
AhlI ACTAGT 1 cut(s) 374
AjnI CCWGG 1 cut(s) 424
AloI GAACNNNNNNTCC 2 cut(s) 798, 830
AluBI AGCT 9 cut(s) 350, 491, 611, 956, 1096, 1130, 1445, 1496, 1650
AluI AGCT 9 cut(s) 350, 491, 611, 956, 1096, 1130, 1445, 1496, 1650
Alw21I GWGCWC 4 cut(s) 352, 1354, 1498, 1652
Alw26I GTCTC 5 cut(s) 269, 532, 639, 1193, 1403
AlwI GGATC 8 cut(s) 121, 134, 216, 1157, 1247, 1248, 1298, 1462
AlwNI CAGNNNCTG 1 cut(s) 1160
Aor13HI TCCGGA 1 cut(s) 849
AoxI GGCC 3 cut(s) 408, 452, 543
ApeKI GCWGC 5 cut(s) 515, 699, 997, 1187, 1442
ApoI RAATTY 1 cut(s) 1532
Asp700I GAANNNNTTC 1 cut(s) 813
AsuHPI GGTGA 2 cut(s) 734, 881
AsuNHI GCTAGC 1 cut(s) 1174
BamHI GGATCC 1 cut(s) 126
BanII GRGCYC 4 cut(s) 352, 723, 1498, 1652
BbsI GAAGAC 1 cut(s) 249
Bbv12I GWGCWC 4 cut(s) 352, 1354, 1498, 1652
BbvCI CCTCAGC 1 cut(s) 1680
BbvI GCAGC 5 cut(s) 527, 711, 1009, 1199, 1454
BccI CCATC 3 cut(s) 64, 1272, 1418
BceAI ACGGC 1 cut(s) 405
BciT130I CCWGG 1 cut(s) 426
BclI TGATCA 2 cut(s) 67, 325
BcoDI GTCTC 5 cut(s) 269, 532, 639, 1193, 1403
BcuI ACTAGT 1 cut(s) 374
BfmI CTRYAG 4 cut(s) 336, 995, 1185, 1587
BfrI CTTAAG 1 cut(s) 979
BfuAI ACCTGC 2 cut(s) 97, 1462
BglII AGATCT 1 cut(s) 913
BisI GCNGC 5 cut(s) 516, 700, 998, 1188, 1443
BlpI GCTNAGC 2 cut(s) 622, 640
BlsI GCNGC 5 cut(s) 517, 701, 999, 1189, 1444
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 1 cut(s) 426
BmiI GGNNCC 4 cut(s) 17, 128, 720, 854
BmrFI CCNGG 1 cut(s) 426
BmrI ACTGGG 2 cut(s) 599, 1409
BmsI GCATC 5 cut(s) 635, 911, 1383, 1387, 1705
BmtI GCTAGC 1 cut(s) 1178
BmuI ACTGGG 2 cut(s) 599, 1409
BpiI GAAGAC 1 cut(s) 249
BplI GAGNNNNNCTC 2 cut(s) 545, 577
BpmI CTGGAG 1 cut(s) 825
Bpu10I CCTNAGC 1 cut(s) 1680
Bpu1102I GCTNAGC 2 cut(s) 622, 640
BpuEI CTTGAG 1 cut(s) 1217
BsaAI YACGTR 3 cut(s) 1261, 1406, 1490
BsaI GGTCTC 3 cut(s) 532, 1193, 1403
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 1 cut(s) 849
BsaXI ACNNNNNCTCC 2 cut(s) 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 1209, 1364
Bse118I RCCGGY 1 cut(s) 1170
Bse1I ACTGG 5 cut(s) 605, 1128, 1149, 1415, 1557
Bse3DI GCAATG 1 cut(s) 517
BseAI TCCGGA 1 cut(s) 849
BseBI CCWGG 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 411
BseLI CCNNNNNNNGG 3 cut(s) 136, 1209, 1364
BseMI GCAATG 1 cut(s) 517
BseNI ACTGG 5 cut(s) 605, 1128, 1149, 1415, 1557
BseRI GAGGAG 5 cut(s) 272, 287, 731, 1323, 1344
BseXI GCAGC 5 cut(s) 527, 711, 1009, 1199, 1454
BshFI GGCC 3 cut(s) 410, 454, 545
BsiHKAI GWGCWC 4 cut(s) 352, 1354, 1498, 1652
BsiSI CCGG 2 cut(s) 850, 1171
BslFI GGGAC 1 cut(s) 1724
BslI CCNNNNNNNGG 3 cut(s) 136, 1209, 1364
BsmAI GTCTC 5 cut(s) 269, 532, 639, 1193, 1403
BsmBI CGTCTC 1 cut(s) 639
BsmFI GGGAC 1 cut(s) 1724
BsmI GAATGC 1 cut(s) 1138
BsnI GGCC 3 cut(s) 410, 454, 545
Bso31I GGTCTC 3 cut(s) 532, 1193, 1403
Bsp1286I GDGCHC 5 cut(s) 352, 723, 1354, 1498, 1652
Bsp13I TCCGGA 1 cut(s) 849
Bsp1720I GCTNAGC 2 cut(s) 622, 640
BspACI CCGC 2 cut(s) 285, 1347
BspANI GGCC 3 cut(s) 410, 454, 545
BspEI TCCGGA 1 cut(s) 849
BspLI GGNNCC 4 cut(s) 17, 128, 720, 854
BspMAI CTGCAG 3 cut(s) 999, 1189, 1591
BspMI ACCTGC 2 cut(s) 97, 1462
BspOI GCTAGC 1 cut(s) 1178
BspPI GGATC 8 cut(s) 121, 134, 216, 1157, 1247, 1248, 1298, 1462
BspTI CTTAAG 1 cut(s) 979
BspTNI GGTCTC 3 cut(s) 532, 1193, 1403
BsrDI GCAATG 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 1170
BsrI ACTGG 5 cut(s) 605, 1128, 1149, 1415, 1557
BssAI RCCGGY 1 cut(s) 1170
BssECI CCNNGG 1 cut(s) 411
BssNAI GTATAC 1 cut(s) 1689
BssT1I CCWWGG 1 cut(s) 411
Bst1107I GTATAC 1 cut(s) 1689
Bst2UI CCWGG 1 cut(s) 426
Bst4CI ACNGT 3 cut(s) 76, 280, 436
BstAFI CTTAAG 1 cut(s) 979
BstAPI GCANNNNNTGC 1 cut(s) 1184
BstBAI YACGTR 3 cut(s) 1261, 1406, 1490
BstC8I GCNNGC 3 cut(s) 348, 1108, 1176
BstMAI GTCTC 5 cut(s) 269, 532, 639, 1193, 1403
BstMWI GCNNNNNNNGC 3 cut(s) 356, 1184, 1607
BstNI CCWGG 1 cut(s) 426
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 1 cut(s) 424
BstSFI CTRYAG 4 cut(s) 336, 995, 1185, 1587
BstSNI TACGTA 2 cut(s) 1261, 1490
BstV1I GCAGC 5 cut(s) 527, 711, 1009, 1199, 1454
BstV2I GAAGAC 1 cut(s) 249
BstX2I RGATCY 4 cut(s) 126, 208, 913, 1454
BstYI RGATCY 4 cut(s) 126, 208, 913, 1454
BstZ17I GTATAC 1 cut(s) 1689
BsuRI GGCC 3 cut(s) 410, 454, 545
BtsI GCAGTG 1 cut(s) 709
BtsIMutI CAGTG 5 cut(s) 709, 1161, 1422, 1543, 1652
BveI ACCTGC 2 cut(s) 97, 1462
Cac8I GCNNGC 3 cut(s) 348, 1108, 1176
CaiI CAGNNNCTG 1 cut(s) 1160
Cfr10I RCCGGY 1 cut(s) 1170
Csp6I GTAC 3 cut(s) 559, 1262, 1619
CviAII CATG 8 cut(s) 79, 302, 323, 329, 589, 727, 1236, 1294
CviQI GTAC 3 cut(s) 559, 1262, 1619
Ecl136II GAGCTC 3 cut(s) 350, 1496, 1650
Eco105I TACGTA 2 cut(s) 1261, 1490
Eco130I CCWWGG 1 cut(s) 411
Eco24I GRGCYC 4 cut(s) 352, 723, 1498, 1652
Eco31I GGTCTC 3 cut(s) 532, 1193, 1403
Eco53kI GAGCTC 3 cut(s) 350, 1496, 1650
Eco57I CTGAAG 1 cut(s) 1623
EcoICRI GAGCTC 3 cut(s) 350, 1496, 1650
EcoRI GAATTC 1 cut(s) 1532
EcoRII CCWGG 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 411
EcoT22I ATGCAT 1 cut(s) 1376
EcoT38I GRGCYC 4 cut(s) 352, 723, 1498, 1652
ErhI CCWWGG 1 cut(s) 411
Esp3I CGTCTC 1 cut(s) 639
FaeI CATG 8 cut(s) 82, 305, 326, 332, 592, 730, 1239, 1297
FalI AAGNNNNNCTT 4 cut(s) 1080, 1112, 1126, 1158
FaqI GGGAC 1 cut(s) 1724
FatI CATG 8 cut(s) 78, 301, 322, 328, 588, 726, 1235, 1293
FauNDI CATATG 1 cut(s) 826
FbaI TGATCA 2 cut(s) 67, 325
FblI GTMKAC 1 cut(s) 1688
Fnu4HI GCNGC 5 cut(s) 516, 700, 998, 1188, 1443
FriOI GRGCYC 4 cut(s) 352, 723, 1498, 1652
Fsp4HI GCNGC 5 cut(s) 516, 700, 998, 1188, 1443
GluI GCNGC 5 cut(s) 516, 700, 998, 1188, 1443
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 3 cut(s) 410, 454, 545
HapII CCGG 2 cut(s) 850, 1171
Hin1II CATG 8 cut(s) 82, 305, 326, 332, 592, 730, 1239, 1297
HincII GTYRAC 1 cut(s) 1524
HindII GTYRAC 1 cut(s) 1524
HindIII AAGCTT 1 cut(s) 1094
HinfI GANTC 3 cut(s) 1232, 1520, 1702
HpaII CCGG 2 cut(s) 850, 1171
HphI GGTGA 2 cut(s) 734, 881
Hpy166II GTNNAC 4 cut(s) 445, 1264, 1524, 1689
Hpy188I TCNGA 9 cut(s) 72, 113, 354, 715, 819, 911, 1012, 1065, 1500
Hpy188III TCNNGA 9 cut(s) 13, 404, 651, 804, 850, 887, 1196, 1307, 1394
Hpy8I GTNNAC 4 cut(s) 445, 1264, 1524, 1689
Hpy99I CGWCG 1 cut(s) 1341
HpyAV CCTTC 5 cut(s) 116, 444, 680, 925, 1125
HpyCH4III ACNGT 3 cut(s) 76, 280, 436
HpyCH4IV ACGT 5 cut(s) 246, 1180, 1260, 1405, 1489
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 1184, 1607
HpySE526I ACGT 5 cut(s) 246, 1180, 1260, 1405, 1489
Hsp92II CATG 8 cut(s) 82, 305, 326, 332, 592, 730, 1239, 1297
Kpn2I TCCGGA 1 cut(s) 849
Ksp22I TGATCA 2 cut(s) 67, 325
LmnI GCTCC 3 cut(s) 680, 718, 1357
Lsp1109I GCAGC 5 cut(s) 527, 711, 1009, 1199, 1454
LweI GCATC 5 cut(s) 635, 911, 1383, 1387, 1705
MaeII ACGT 5 cut(s) 246, 1180, 1260, 1405, 1489
MaeIII GTNAC 6 cut(s) 331, 661, 704, 740, 1156, 1639
MflI RGATCY 4 cut(s) 126, 208, 913, 1454
MhlI GDGCHC 5 cut(s) 352, 723, 1354, 1498, 1652
MluCI AATT 6 cut(s) 144, 836, 969, 1013, 1213, 1532
MlyI GAGTC 2 cut(s) 1241, 1529
Mph1103I ATGCAT 1 cut(s) 1376
MroI TCCGGA 1 cut(s) 849
MroXI GAANNNNTTC 1 cut(s) 813
MseI TTAA 3 cut(s) 839, 980, 1476
MslI CAYNNNNRTG 2 cut(s) 327, 593
MspA1I CMGCKG 2 cut(s) 285, 1445
MspCI CTTAAG 1 cut(s) 979
MspI CCGG 2 cut(s) 850, 1171
MspR9I CCNGG 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 1138
MvaI CCWGG 1 cut(s) 426
MwoI GCNNNNNNNGC 3 cut(s) 356, 1184, 1607
NdeI CATATG 1 cut(s) 826
NheI GCTAGC 1 cut(s) 1174
NlaIII CATG 8 cut(s) 82, 305, 326, 332, 592, 730, 1239, 1297
NlaIV GGNNCC 4 cut(s) 17, 128, 720, 854
NmeAIII GCCGAG 1 cut(s) 509
NmuCI GTSAC 4 cut(s) 661, 704, 740, 1156
NsiI ATGCAT 1 cut(s) 1376
NspI RCATGY 1 cut(s) 730
PctI GAATGC 1 cut(s) 1138
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 1 cut(s) 1702
PflMI CCANNNNNTGG 1 cut(s) 1364
PkrI GCNGC 5 cut(s) 517, 701, 999, 1189, 1444
PleI GAGTC 2 cut(s) 1240, 1528
PpsI GAGTC 2 cut(s) 1240, 1528
Ppu21I YACGTR 3 cut(s) 1261, 1406, 1490
Psp124BI GAGCTC 3 cut(s) 352, 1498, 1652
Psp6I CCWGG 1 cut(s) 424
PspGI CCWGG 1 cut(s) 424
PspN4I GGNNCC 4 cut(s) 17, 128, 720, 854
PstI CTGCAG 3 cut(s) 999, 1189, 1591
PstNI CAGNNNCTG 1 cut(s) 1160
PsuI RGATCY 4 cut(s) 126, 208, 913, 1454
PvuII CAGCTG 1 cut(s) 1445
RsaI GTAC 3 cut(s) 560, 1263, 1620
RsaNI GTAC 3 cut(s) 559, 1262, 1619
RseI CAYNNNNRTG 2 cut(s) 327, 593
SacI GAGCTC 3 cut(s) 352, 1498, 1652
SaqAI TTAA 3 cut(s) 839, 980, 1476
SatI GCNGC 5 cut(s) 516, 700, 998, 1188, 1443
ScaI AGTACT 1 cut(s) 560
SchI GAGTC 2 cut(s) 1241, 1529
ScrFI CCNGG 1 cut(s) 426
SduI GDGCHC 5 cut(s) 352, 723, 1354, 1498, 1652
SfaNI GCATC 5 cut(s) 635, 911, 1383, 1387, 1705
SfcI CTRYAG 4 cut(s) 336, 995, 1185, 1587
SmiMI CAYNNNNRTG 2 cut(s) 327, 593
SmlI CTYRAG 2 cut(s) 979, 1196
SmoI CTYRAG 2 cut(s) 979, 1196
SnaBI TACGTA 2 cut(s) 1261, 1490
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 6 cut(s) 144, 836, 969, 1013, 1213, 1532
SsiI CCGC 2 cut(s) 285, 1347
SspI AATATT 1 cut(s) 1362
SstI GAGCTC 3 cut(s) 352, 1498, 1652
StyD4I CCNGG 1 cut(s) 424
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 3 cut(s) 76, 280, 436
TaiI ACGT 5 cut(s) 249, 1183, 1263, 1408, 1492
TaqI TCGA 5 cut(s) 403, 938, 1083, 1230, 1518
TaqII GACCGA 1 cut(s) 1217
TasI AATT 6 cut(s) 144, 836, 969, 1013, 1213, 1532
TatI WGTACW 1 cut(s) 558
TfiI GAWTC 1 cut(s) 1702
Tru1I TTAA 3 cut(s) 839, 980, 1476
Tru9I TTAA 3 cut(s) 839, 980, 1476
TscAI CASTG 5 cut(s) 709, 1161, 1422, 1543, 1659
TseFI GTSAC 4 cut(s) 661, 704, 740, 1156
TseI GCWGC 5 cut(s) 515, 699, 997, 1187, 1442
Tsp45I GTSAC 4 cut(s) 661, 704, 740, 1156
TspDTI ATGAA 9 cut(s) 15, 318, 591, 605, 699, 708, 944, 1002, 1101
TspGWI ACGGA 1 cut(s) 1277
TspRI CASTG 5 cut(s) 709, 1161, 1422, 1543, 1659
Van91I CCANNNNNTGG 1 cut(s) 1364
Vha464I CTTAAG 1 cut(s) 979
XapI RAATTY 1 cut(s) 1532
XbaI TCTAGA 2 cut(s) 886, 1393
XceI RCATGY 1 cut(s) 730
XmiI GTMKAC 1 cut(s) 1688
XmnI GAANNNNTTC 1 cut(s) 813
ZrmI AGTACT 1 cut(s) 560
Zsp2I ATGCAT 1 cut(s) 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.