Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0014583

Physical Location & Seq

Genomic Coordinates Forward (+)

26483434 .. 26484714

Gene Structure 1281 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1249100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1206 bp

ATGTTAAATTCCATTGTAGAGCAGCCTTTGCACGGTTTGGATGATTTCTTTGGTTCTGTGAAGTTTGAGTTATTTGAAGTTGATTTAATTAGTTTGTTGAAAGCATTAGATCTTTCTGGCAATTGGGAAAGAGCCATTTTGTTGTTTGAATGGATTCTGTCCAACTTAAGCTCTGAAAATTTGAAATTAAATAACCAGATGATTGAACTAATGGTTAGAATTCTTGGGAGAGAGTCGCAGCATGCTATTGCATCGAAGCTGTTCGATGTAATTCCCATAGAAGAATACTCGCTGGATATCCGAGCTTACACGACTATCCTTCATGCGCACTCTCGTAGTGGTAAGTACGAAAGTGCAATTGACTTGTTTGACAAAATGATGGAAAATGGTATGTCACCAACTTTGGTGACTTACAATGTCATGCTTGATGTATATGGGAAGATGGGTCGATCTTGGGATAAGATTTTAGGACTCTTGGATGAGATGAGGAGGAAGGGATTGGAATTTGATGAGTTTACTTGTAGTACGGTGATATCTGCTTGTGGGCGAGAGGGTTTGTTGAACGAGGCAAATGAGTTATTTGTCGGGTTAACGTCACAAGGCTATGTACCTGGAAATGTTACCTATAATGCTTTGTTACAAGTTTTTGGCAAGGCAGGGGTATTTACGGAGGCCTTGAGCATATTAAAAGAAATGGAGGATAATAATTGCCCACCTGATGCTGTTACTTACAATGAGCTTGTGGCAGCTTATGTGAGGGCAGGATTCTTTGAGGAAGGTGCATCTGTCATAGAAACGATGACCCAAAAAGGAACAATGCCAAATGCTGTTACATACACAACTGTGATAAATGCATATGGTAAAGCTAGAAATGAGGAAGAGGCTCTAAGGTTGTTCAATCATATGAAGGCGACAGGTTGTGTTCCTAATGTTTGCACATTCAATGCTGTTCTCCGAATGCTGGGAAAGAAGTCACTGCCCGAGGAGATGATAAAGTTACTTTGTGAAATGAAGTCAAGTGGATGTGCCCCCAACCGTATTACATGGAACACAATGCTTGCCATGTGTGGTGATAAGGGTAGGCACAAGTATGTGAACCAGGTATTTCAAGAAATGAAGAATTGTGGTTTTCAGCCAGACAGAGACACATTCAATACCTTAATCAGTGCATATGGACGGTGTGGATCAAAATTAGACGCTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

402

Amino Acids

44.89

Weight (kDa)

5.21

Isoelectric Point (pI)

31.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	74 - 194	3.9e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	95 - 148	3.7e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	99 - 145	2e-13	PPR repeat family
PPR	PF01535	102 - 130	9.4e-07	PPR repeat
PPR_2	PF13041	133 - 182	3.6e-11	PPR repeat family
PPR_3	PF13812	158 - 218	2e-08	Pentatricopeptide repeat domain
TPR_24	PF23276	173 - 250	7.4e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	204 - 245	1e-11	PPR repeat family
PPR	PF01535	207 - 237	1.1e-06	PPR repeat
PPR_2	PF13041	239 - 287	2.5e-11	PPR repeat family
TPR_24	PF23276	261 - 340	3.7e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	263 - 323	1.6e-15	Pentatricopeptide repeat domain
PPR_1	PF12854	270 - 303	6.6e-08	PPR repeat
PPR_2	PF13041	274 - 322	1.4e-14	PPR repeat family
PPR	PF01535	277 - 307	7.5e-08	PPR repeat
PPR_long	PF17177	294 - 396	2.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	309 - 356	5.7e-12	PPR repeat family
PPR_2	PF13041	344 - 391	7.4e-12	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18940
fragaria_vesca	FvH4_4g07880
malus_domestica	MD13G1249100.v1.1 MD16G1255600.v1.1
prunus_persica	Prupe.3G037500_v2.0.a1
pyrus_communis	pycom13g21970 pycom16g21670
rosa_chinensis	RchiOBHm_Chr4g0401161
rosa_laevigata	RLG00000009158
rosa_multiflora	Rmu_sc0003257.1_g000006
rosa_roxburghii	Rroxscaffold_5G00346010
rosa_rugosa	Rorug04G0028400
rosa_samantha	Rh4AG105900 Rh4BG100900 Rh4CG114600 Rh4DG098100
rosa_wichuraiana	Rw4G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	327
AclWI	GGATC	1 cut(s)	1192
AcsI	RAATTY	4 cut(s)	7, 178, 219, 503
AfaI	GTAC	3 cut(s)	347, 526, 609
AfiI	CCNNNNNNNGG	2 cut(s)	32, 961
AflII	CTTAAG	1 cut(s)	166
AjnI	CCWGG	2 cut(s)	610, 1098
AleI	CACNNNNGTG	1 cut(s)	842
AluBI	AGCT	6 cut(s)	171, 259, 305, 739, 749, 866
AluI	AGCT	6 cut(s)	171, 259, 305, 739, 749, 866
Alw26I	GTCTC	1 cut(s)	1137
AlwI	GGATC	1 cut(s)	1192
Ama87I	CYCGRG	1 cut(s)	980
AoxI	GGCC	1 cut(s)	672
ApeKI	GCWGC	4 cut(s)	22, 238, 746, 1199
ApoI	RAATTY	4 cut(s)	7, 178, 219, 503
Asp700I	GAANNNNTTC	2 cut(s)	153, 260
AspLEI	GCGC	1 cut(s)	328
AsuHPI	GGTGA	4 cut(s)	387, 418, 541, 1082
AvaI	CYCGRG	1 cut(s)	980
BaeGI	GKGCMC	1 cut(s)	1030
BbvI	GCAGC	4 cut(s)	34, 250, 758, 1186
BccI	CCATC	2 cut(s)	373, 436
BciT130I	CCWGG	2 cut(s)	612, 1100
BcoDI	GTCTC	1 cut(s)	1137
BfaI	CTAG	1 cut(s)	867
BfrI	CTTAAG	1 cut(s)	166
BglII	AGATCT	1 cut(s)	109
BisI	GCNGC	4 cut(s)	23, 239, 747, 1200
BlsI	GCNGC	4 cut(s)	24, 240, 748, 1201
Bme1390I	CCNGG	2 cut(s)	612, 1100
BmeT110I	CYCGRG	1 cut(s)	980
BmrFI	CCNGG	2 cut(s)	612, 1100
BmsI	GCATC	3 cut(s)	260, 709, 791
BpuEI	CTTGAG	1 cut(s)	697
BsaJI	CCNNGG	1 cut(s)	981
Bsc4I	CCNNNNNNNGG	2 cut(s)	32, 961
BseBI	CCWGG	2 cut(s)	612, 1100
BseDI	CCNNGG	1 cut(s)	981
BseGI	GGATG	3 cut(s)	46, 484, 1028
BseLI	CCNNNNNNNGG	2 cut(s)	32, 961
BseRI	GAGGAG	2 cut(s)	502, 998
BseSI	GKGCMC	1 cut(s)	1030
BseXI	GCAGC	4 cut(s)	34, 250, 758, 1186
BseYI	CCCAGC	1 cut(s)	961
BshFI	GGCC	1 cut(s)	674
BsiHKCI	CYCGRG	1 cut(s)	980
BslI	CCNNNNNNNGG	2 cut(s)	32, 961
BsmAI	GTCTC	1 cut(s)	1137
BsmI	GAATGC	1 cut(s)	963
BsnI	GGCC	1 cut(s)	674
BsoBI	CYCGRG	1 cut(s)	980
Bsp1286I	GDGCHC	1 cut(s)	1030
Bsp143I	GATC	3 cut(s)	109, 449, 1184
BspANI	GGCC	1 cut(s)	674
BspPI	GGATC	1 cut(s)	1192
BspTI	CTTAAG	1 cut(s)	166
BssECI	CCNNGG	1 cut(s)	981
BssMI	GATC	3 cut(s)	109, 449, 1184
Bst2UI	CCWGG	2 cut(s)	612, 1100
Bst4CI	ACNGT	5 cut(s)	35, 529, 844, 1037, 1179
Bst6I	CTCTTC	1 cut(s)	873
BstAFI	CTTAAG	1 cut(s)	166
BstAPI	GCANNNNNTGC	1 cut(s)	28
BstC8I	GCNNGC	2 cut(s)	243, 1059
BstDEI	CTNAG	1 cut(s)	887
BstF5I	GGATG	3 cut(s)	46, 484, 1028
BstHHI	GCGC	1 cut(s)	328
BstKTI	GATC	3 cut(s)	112, 452, 1187
BstMAI	GTCTC	1 cut(s)	1137
BstMBI	GATC	3 cut(s)	109, 449, 1184
BstMWI	GCNNNNNNNGC	1 cut(s)	28
BstNI	CCWGG	2 cut(s)	612, 1100
BstNSI	RCATGY	1 cut(s)	245
BstSCI	CCNGG	2 cut(s)	610, 1098
BstSLI	GKGCMC	1 cut(s)	1030
BstV1I	GCAGC	4 cut(s)	34, 250, 758, 1186
BstX2I	RGATCY	1 cut(s)	109
BstYI	RGATCY	1 cut(s)	109
BsuRI	GGCC	1 cut(s)	674
BtsCI	GGATG	3 cut(s)	46, 484, 1028
BtsI	GCAGTG	1 cut(s)	974
BtsIMutI	CAGTG	2 cut(s)	974, 1171
Cac8I	GCNNGC	2 cut(s)	243, 1059
CfoI	GCGC	1 cut(s)	328
CsiI	ACCWGGT	1 cut(s)	1098
Csp6I	GTAC	3 cut(s)	346, 525, 608
CviAII	CATG	5 cut(s)	242, 323, 421, 1044, 1063
CviQI	GTAC	3 cut(s)	346, 525, 608
DdeI	CTNAG	1 cut(s)	887
DpnI	GATC	3 cut(s)	111, 451, 1186
DpnII	GATC	3 cut(s)	109, 449, 1184
Eam1104I	CTCTTC	1 cut(s)	873
EarI	CTCTTC	1 cut(s)	873
Eco147I	AGGCCT	1 cut(s)	674
Eco32I	GATATC	2 cut(s)	298, 534
Eco88I	CYCGRG	1 cut(s)	980
EcoRI	GAATTC	1 cut(s)	219
EcoRII	CCWGG	2 cut(s)	610, 1098
EcoRV	GATATC	2 cut(s)	298, 534
EcoT22I	ATGCAT	1 cut(s)	856
FaeI	CATG	5 cut(s)	245, 326, 424, 1047, 1066
FatI	CATG	5 cut(s)	241, 322, 420, 1043, 1062
FauNDI	CATATG	3 cut(s)	856, 903, 1171
Fnu4HI	GCNGC	4 cut(s)	23, 239, 747, 1200
FokI	GGATG	3 cut(s)	53, 491, 1035
Fsp4HI	GCNGC	4 cut(s)	23, 239, 747, 1200
FspAI	RTGCGCAY	1 cut(s)	327
FspBI	CTAG	1 cut(s)	867
FspI	TGCGCA	1 cut(s)	327
GlaI	GCGC	1 cut(s)	327
GluI	GCNGC	4 cut(s)	23, 239, 747, 1200
GsaI	CCCAGC	1 cut(s)	965
HaeIII	GGCC	1 cut(s)	674
HhaI	GCGC	1 cut(s)	328
Hin1II	CATG	5 cut(s)	245, 326, 424, 1047, 1066
Hin6I	GCGC	1 cut(s)	326
HinP1I	GCGC	1 cut(s)	326
HincII	GTYRAC	1 cut(s)	591
HindII	GTYRAC	1 cut(s)	591
HinfI	GANTC	4 cut(s)	154, 233, 471, 765
HpaI	GTTAAC	1 cut(s)	591
HphI	GGTGA	4 cut(s)	387, 418, 541, 1082
Hpy166II	GTNNAC	3 cut(s)	516, 591, 1096
Hpy188I	TCNGA	3 cut(s)	175, 302, 956
Hpy188III	TCNNGA	1 cut(s)	1109
Hpy8I	GTNNAC	3 cut(s)	516, 591, 1096
HpyAV	CCTTC	4 cut(s)	329, 487, 770, 901
HpyCH4III	ACNGT	5 cut(s)	35, 529, 844, 1037, 1179
HpyCH4IV	ACGT	1 cut(s)	593
HpyCH4V	TGCA	8 cut(s)	31, 251, 356, 782, 854, 936, 1169, 1202
HpyF10VI	GCNNNNNNNGC	1 cut(s)	28
HpyF3I	CTNAG	1 cut(s)	887
HpySE526I	ACGT	1 cut(s)	593
Hsp92II	CATG	5 cut(s)	245, 326, 424, 1047, 1066
HspAI	GCGC	1 cut(s)	326
KspAI	GTTAAC	1 cut(s)	591
Kzo9I	GATC	3 cut(s)	109, 449, 1184
Lsp1109I	GCAGC	4 cut(s)	34, 250, 758, 1186
LweI	GCATC	3 cut(s)	260, 709, 791
MabI	ACCWGGT	1 cut(s)	1098
MaeI	CTAG	1 cut(s)	867
MaeII	ACGT	1 cut(s)	593
MaeIII	GTNAC	9 cut(s)	393, 406, 594, 619, 636, 724, 829, 972, 996
MalI	GATC	3 cut(s)	111, 451, 1186
MboI	GATC	3 cut(s)	109, 449, 1184
MboII	GAAGA	4 cut(s)	293, 451, 890, 1129
MfeI	CAATTG	2 cut(s)	121, 357
MflI	RGATCY	1 cut(s)	109
MhlI	GDGCHC	1 cut(s)	1030
MlyI	GAGTC	2 cut(s)	242, 465
MmeI	TCCRAC	1 cut(s)	186
Mph1103I	ATGCAT	1 cut(s)	856
MroXI	GAANNNNTTC	2 cut(s)	153, 260
MseI	TTAA	7 cut(s)	5, 86, 167, 188, 590, 686, 1160
MslI	CAYNNNNRTG	2 cut(s)	842, 1089
MspCI	CTTAAG	1 cut(s)	166
MspR9I	CCNGG	2 cut(s)	612, 1100
MunI	CAATTG	2 cut(s)	121, 357
Mva1269I	GAATGC	1 cut(s)	963
MvaI	CCWGG	2 cut(s)	612, 1100
MwoI	GCNNNNNNNGC	1 cut(s)	28
NdeI	CATATG	3 cut(s)	856, 903, 1171
NdeII	GATC	3 cut(s)	109, 449, 1184
NlaIII	CATG	5 cut(s)	245, 326, 424, 1047, 1066
NmuCI	GTSAC	4 cut(s)	393, 406, 594, 972
NsbI	TGCGCA	1 cut(s)	327
NsiI	ATGCAT	1 cut(s)	856
NspI	RCATGY	1 cut(s)	245
OliI	CACNNNNGTG	1 cut(s)	842
PaeI	GCATGC	1 cut(s)	245
PceI	AGGCCT	1 cut(s)	674
PctI	GAATGC	1 cut(s)	963
PdmI	GAANNNNTTC	2 cut(s)	153, 260
PfeI	GAWTC	2 cut(s)	154, 765
PkrI	GCNGC	4 cut(s)	24, 240, 748, 1201
PleI	GAGTC	2 cut(s)	241, 465
PpsI	GAGTC	2 cut(s)	241, 465
Psp6I	CCWGG	2 cut(s)	610, 1098
PspFI	CCCAGC	1 cut(s)	961
PspGI	CCWGG	2 cut(s)	610, 1098
PsuI	RGATCY	1 cut(s)	109
RsaI	GTAC	3 cut(s)	347, 526, 609
RsaNI	GTAC	3 cut(s)	346, 525, 608
RseI	CAYNNNNRTG	2 cut(s)	842, 1089
SaqAI	TTAA	7 cut(s)	5, 86, 167, 188, 590, 686, 1160
SatI	GCNGC	4 cut(s)	23, 239, 747, 1200
Sau3AI	GATC	3 cut(s)	109, 449, 1184
SchI	GAGTC	2 cut(s)	242, 465
ScrFI	CCNGG	2 cut(s)	612, 1100
SduI	GDGCHC	1 cut(s)	1030
SexAI	ACCWGGT	1 cut(s)	1098
SfaNI	GCATC	3 cut(s)	260, 709, 791
SmiMI	CAYNNNNRTG	2 cut(s)	842, 1089
SmlI	CTYRAG	2 cut(s)	166, 676
SmoI	CTYRAG	2 cut(s)	166, 676
SphI	GCATGC	1 cut(s)	245
SseBI	AGGCCT	1 cut(s)	674
SspMI	CTAG	1 cut(s)	867
StuI	AGGCCT	1 cut(s)	674
StyD4I	CCNGG	2 cut(s)	610, 1098
TaaI	ACNGT	5 cut(s)	35, 529, 844, 1037, 1179
TaiI	ACGT	1 cut(s)	596
TaqI	TCGA	3 cut(s)	254, 264, 448
TfiI	GAWTC	2 cut(s)	154, 765
Tru1I	TTAA	7 cut(s)	5, 86, 167, 188, 590, 686, 1160
Tru9I	TTAA	7 cut(s)	5, 86, 167, 188, 590, 686, 1160
TscAI	CASTG	2 cut(s)	981, 1171
TseFI	GTSAC	4 cut(s)	393, 406, 594, 972
TseI	GCWGC	4 cut(s)	22, 238, 746, 1199
Tsp45I	GTSAC	4 cut(s)	393, 406, 594, 972
TspDTI	ATGAA	4 cut(s)	311, 920, 1025, 1130
TspGWI	ACGGA	1 cut(s)	683
TspRI	CASTG	2 cut(s)	981, 1171
Vha464I	CTTAAG	1 cut(s)	166
XapI	RAATTY	4 cut(s)	7, 178, 219, 503
XceI	RCATGY	1 cut(s)	245
XmnI	GAANNNNTTC	2 cut(s)	153, 260
XspI	CTAG	1 cut(s)	867
Zsp2I	ATGCAT	1 cut(s)	856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.