Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014583

Physical Location & Seq

Genomic Coordinates Forward (+)

29392303 .. 29394546

Gene Structure 2244 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1255600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGAAGAAACCCACTTCAGTTTTAGTCCCAAATTTCGAGGACGATCGAGAGCTGCTGAAATCAGGAGATGGGGTTCTCGACTTTTTGACTATAAAGGGTAAGTTGATGTTCAATTCCATTGTAGAGCAGCCTTTGCATGGTTTGGACTATTACTTTGGTTCTGTGAAGTTTGAGTTACTTGAAGTTGATTTGATTAGTTTGTTGAAAGCATTAGACCTTTCTGGCAATTGGGAAAGAGCATTTTTGTTGTTTGAATGGATTCTGTCCAACGTAAGCTCTGAAAATTTAAAATTAAATAGCCAGATGATTGAGTTAATGGTTAGAATTCTTGGGAGAGAGTCGCAGCATACGATTGCATCGAAGCTGTTTGATGTAATTCCCATAGAAGAATACACGCTGGATGTCCGAGCTTACACGACGATCCTTCATGCGCACTCTCGTACTGGTAAGTACGAAAGTGCAATTGACTTGTTTGACAAAATGGTGGAAATTGGAATCTCACCAACTTTGGTCACTTACAATGTCATGCTTGATGTTTATGGGAAGATGGGTCGATCTTGGGATAAGATTTTAGGCCTCTTGGATGAGATGAGGAACAAGGGATTTGAATTTGATGAGTTTACTTGTAGTACGGTGATATCTGCCTGTGGAAGAGAGGGTTTGTTGAATGAAGCAAATGAGTTCTTTATTGGATTAAAGTCACAAGGGTATGTACCCGGAACTGTAACTTATAATGCGTTGTTACAAGTTTTTGGCAAGGCAGGGGTATTTACAGAGGCCTTGAGCATATTAAAAGAAATGGAGGATAATAATTGCCCACCTGATGCTGTTACTTACAATGAGCTTGTGGCAGCTTATGTGAGGGCAGGATTCTCCGAGGAAGGTGCATCTGTCATAGAGACGATGACCCAAAAAGGAACAATGCCAAATGCTGTTACATACACAACTGTGATAAATGCATACGGTAAAGCTGGAAAGGAGGAGGAGGCTCTAAGGTTGTTCAACCGTATGAAGGCAACAGGTTGTGTTCCTAATGTTTGCACATACAATGCTGTTCTCGGACTGCTGGGAAAGAAGTCACTGCCCGAGGAGATGATAAAGATACTTTGTGAAATGAAGTCAAGTGGTTGTGCCCCAAACCGTATTACATGGAACACAATGCTTGCCATGTGTGGTGATAAGGGTAGGCACAAGTATGTGAACCAGGTATTTCGAGAAATGAAGAATTGTGGTTTTGAGCCTGACAGAGACACATTCAATACCTTGATCAGTGCATATGGACGGTGTGGATCAGAAATAGATGCTGCACAGATGTACGATGAGATGATCAAAGCAGGATTTACTCCATGCGTTACAACTTACAATGCACTTCTAAATGCTCTGGCACGGCGAGGGGACTGGAGAGCAGCAGAAGCTGCTATGCTAGACATGAGAAATAAGGGCTTCAAGCCTAATGAAACCTCGTATTCATTGATGATCAACTGTTATGCTAAAGGAGGGAATGTAAAGGGGATCGAGAGAATTGAGAGAGAAATTTATGGTGGTTATATTTTTCCCAGCTGGATTCTTTTGAGAACGCTTATTCTTGCAAATTTCAAGTGTAGAGCCCTGGACAGCATGGAGAGGGCATTCCATCAATTGCAGAACATCGGATACAAACCTGACTTGGTTGTATTCAACTCCATGCTTTCAATTTTTGCTAGAAACAACATGTATGATCGGGCCAATGACATGTTGTACATGATTCGTGAAAACGGCCTTCAGCCAGATCTCGTAACTTACAATAGTTTGATGGACATGTACGCCAGAAAGGGAGAGTGCTGGAAAGCAGAAGAAATCCTCATGGCCCTACAAAATTCTGGTGGGAAACCAGACCTTGTCTCTTATAACACAGTCATCAAAGGGTTTTGCAGGCAAGGGCACATGCAGGAGGCCATAAGAATTCTCTCGGAGATGACAACTAGAGGAATTCGGCCGTGTATTTTCACCTACAATACTTTCATCACAGGCTATGCAGGGCAGGGAATGTTCTCAGAAATAGATGAAGTGATTAGCTATATGACTCAGAACAATTGCAGGCCGAATGAGCTGAGCTACAAGATTGCGGTGGATGGTTATTGTAAAGCGAGAAAATATAAAGAAGCCATAGACTTTCTGTCCACGATTAAGGAGATTGATAGTTCGATTGATGATCAATATGTGCAAAGACTTGCTTCTCGCATTCGGGGCAATTTGGATTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

748

Amino Acids

84.36

Weight (kDa)

5.64

Isoelectric Point (pI)

30.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	109 - 226	4.1e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	130 - 183	9.4e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	134 - 180	1.1e-12	PPR repeat family
PPR	PF01535	137 - 166	4.7e-06	PPR repeat
PPR_2	PF13041	168 - 217	1.5e-10	PPR repeat family
PPR_3	PF13812	193 - 253	4e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	205 - 248	9.7e-06	PPR repeat family
TPR_24	PF23276	208 - 285	1.9e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	222 - 328	3.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	239 - 286	1.6e-12	PPR repeat family
PPR	PF01535	242 - 272	2.3e-06	PPR repeat
PPR_3	PF13812	262 - 323	7e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	274 - 322	2.1e-10	PPR repeat family
PPR_long	PF17177	294 - 421	1.6e-07	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	295 - 376	2.9e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	298 - 358	4.9e-16	Pentatricopeptide repeat domain
PPR_1	PF12854	305 - 338	2.2e-09	PPR repeat
PPR_2	PF13041	309 - 353	9.5e-15	PPR repeat family
PPR	PF01535	312 - 342	3.4e-09	PPR repeat
PPR_long	PF17177	329 - 466	1.1e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	344 - 391	1.5e-11	PPR repeat family
PPR_2	PF13041	379 - 426	1.5e-11	PPR repeat family
PPR	PF01535	418 - 447	4.4e-06	PPR repeat
PPR_3	PF13812	439 - 497	3.1e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	449 - 498	3.2e-13	PPR repeat family
TPR_24	PF23276	543 - 648	1.4e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	544 - 601	1.2e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	554 - 603	2.9e-09	PPR repeat family
PPR_1	PF12854	585 - 614	1e-05	PPR repeat
TPR_24	PF23276	599 - 659	7.6e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	613 - 671	2.8e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	621 - 653	8e-12	PPR repeat
PPR_2	PF13041	624 - 659	8.3e-11	PPR repeat family
PPR	PF01535	627 - 657	4.3e-08	PPR repeat
PPR_3	PF13812	647 - 700	3.1e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	659 - 708	4.4e-12	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014583)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18940
fragaria_vesca	FvH4_4g07880
malus_domestica	MD13G1249100.v1.1 MD16G1255600.v1.1
prunus_persica	Prupe.3G037500_v2.0.a1
pyrus_communis	pycom13g21970 pycom16g21670
rosa_chinensis	RchiOBHm_Chr4g0401161
rosa_laevigata	RLG00000009158
rosa_multiflora	Rmu_sc0003257.1_g000006
rosa_roxburghii	Rroxscaffold_5G00346010
rosa_rugosa	Rorug04G0028400
rosa_samantha	Rh4AG105900 Rh4BG100900 Rh4CG114600 Rh4DG098100
rosa_wichuraiana	Rw4G008590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	732, 1887
Acc16I	TGCGCA	1 cut(s)	432
AciI	CCGC	1 cut(s)	2105
AclWI	GGATC	3 cut(s)	415, 1297, 1520
AcoI	YGGCCR	1 cut(s)	1973
AcsI	RAATTY	9 cut(s)	31, 283, 324, 608, 1533, 1591, 1855, 1941, 1968
AcuI	CTGAAG	1 cut(s)	1745
AfaI	GTAC	7 cut(s)	442, 452, 631, 714, 1316, 1739, 1802
AfiI	CCNNNNNNNGG	2 cut(s)	137, 1012
AflIII	ACRYGT	3 cut(s)	1710, 1731, 1797
AhdI	GACNNNNNGTC	1 cut(s)	2155
AjnI	CCWGG	2 cut(s)	1203, 1608
AleI	CACNNNNGTG	1 cut(s)	947
AloI	GAACNNNNNNTCC	2 cut(s)	57, 89
Alw26I	GTCTC	3 cut(s)	893, 1242, 1885
AlwI	GGATC	3 cut(s)	415, 1297, 1520
AlwNI	CAGNNNCTG	1 cut(s)	1415
Ama87I	CYCGRG	1 cut(s)	1085
AoxI	GGCC	8 cut(s)	574, 777, 1722, 1756, 1845, 1932, 1973, 2078
ApeKI	GCWGC	7 cut(s)	52, 127, 343, 851, 1304, 1406, 1415
ApoI	RAATTY	9 cut(s)	31, 283, 324, 608, 1533, 1591, 1855, 1941, 1968
ArsI	GACNNNNNNTTYG	2 cut(s)	137, 169
Asp700I	GAANNNNTTC	1 cut(s)	258
AspLEI	GCGC	1 cut(s)	433
AspS9I	GGNCC	2 cut(s)	1722, 1846
AsuC2I	CCSGG	1 cut(s)	717
AsuHPI	GGTGA	4 cut(s)	492, 646, 1187, 1978
AvaI	CYCGRG	1 cut(s)	1085
BaeGI	GKGCMC	2 cut(s)	1135, 1923
BanII	GRGCYC	1 cut(s)	1609
BbvI	GCAGC	7 cut(s)	39, 139, 355, 863, 1291, 1402, 1418
BccI	CCATC	5 cut(s)	62, 541, 1641, 1786, 2105
BceAI	ACGGC	3 cut(s)	1403, 1771, 1960
BcgI	CGANNNNNNTGC	2 cut(s)	866, 900
BciT130I	CCWGG	2 cut(s)	1205, 1610
BciVI	GTATCC	1 cut(s)	1646
BclI	TGATCA	4 cut(s)	1266, 1326, 1476, 2191
BcnI	CCSGG	1 cut(s)	717
BcoDI	GTCTC	3 cut(s)	893, 1242, 1885
BfaI	CTAG	3 cut(s)	1424, 1701, 1962
BfuI	GTATCC	1 cut(s)	1646
BglII	AGATCT	1 cut(s)	1768
BisI	GCNGC	7 cut(s)	53, 128, 344, 852, 1305, 1407, 1416
BlpI	GCTNAGC	1 cut(s)	2090
BlsI	GCNGC	7 cut(s)	54, 129, 345, 853, 1306, 1408, 1417
Bme1390I	CCNGG	3 cut(s)	717, 1205, 1610
BmeRI	GACNNNNNGTC	1 cut(s)	2155
BmeT110I	CYCGRG	1 cut(s)	1085
BmgT120I	GGNCC	2 cut(s)	1722, 1846
BmrFI	CCNGG	3 cut(s)	717, 1205, 1610
BmsI	GCATC	4 cut(s)	365, 814, 896, 1291
BpmI	CTGGAG	1 cut(s)	1420
Bpu1102I	GCTNAGC	1 cut(s)	2090
BpuEI	CTTGAG	1 cut(s)	802
BpuMI	CCSGG	1 cut(s)	717
BsaJI	CCNNGG	3 cut(s)	876, 1086, 1608
BsaXI	ACNNNNNCTCC	4 cut(s)	57, 87, 1488, 1518
Bsc4I	CCNNNNNNNGG	2 cut(s)	137, 1012
Bse1I	ACTGG	2 cut(s)	448, 1403
BseBI	CCWGG	2 cut(s)	1205, 1610
BseDI	CCNNGG	3 cut(s)	876, 1086, 1608
BseGI	GGATG	3 cut(s)	406, 589, 2116
BseLI	CCNNNNNNNGG	2 cut(s)	137, 1012
BseMII	CTCAG	3 cut(s)	2046, 2078, 2081
BseNI	ACTGG	2 cut(s)	448, 1403
BseRI	GAGGAG	3 cut(s)	995, 998, 1103
BseSI	GKGCMC	2 cut(s)	1135, 1923
BseX3I	CGGCCG	1 cut(s)	1973
BseXI	GCAGC	7 cut(s)	39, 139, 355, 863, 1291, 1402, 1418
BseYI	CCCAGC	2 cut(s)	1066, 1556
BsgI	GTGCAG	1 cut(s)	1290
Bsh1285I	CGRYCG	2 cut(s)	46, 1976
BshFI	GGCC	8 cut(s)	576, 779, 1724, 1758, 1847, 1934, 1975, 2080
BsiEI	CGRYCG	2 cut(s)	46, 1976
BsiHKCI	CYCGRG	1 cut(s)	1085
BsiSI	CCGG	1 cut(s)	717
BslFI	GGGAC	2 cut(s)	11, 1409
BslI	CCNNNNNNNGG	2 cut(s)	137, 1012
BsmAI	GTCTC	3 cut(s)	893, 1242, 1885
BsmBI	CGTCTC	1 cut(s)	893
BsmFI	GGGAC	2 cut(s)	11, 1409
BsmI	GAATGC	2 cut(s)	1628, 2220
BsnI	GGCC	8 cut(s)	576, 779, 1724, 1758, 1847, 1934, 1975, 2080
BsoBI	CYCGRG	1 cut(s)	1085
Bsp1286I	GDGCHC	3 cut(s)	1135, 1609, 1923
Bsp1407I	TGTACA	1 cut(s)	1737
Bsp1720I	GCTNAGC	1 cut(s)	2090
BspACI	CCGC	1 cut(s)	2105
BspANI	GGCC	8 cut(s)	576, 779, 1724, 1758, 1847, 1934, 1975, 2080
BspCNI	CTCAG	3 cut(s)	2045, 2077, 2082
BspHI	TCATGA	1 cut(s)	2240
BspPI	GGATC	3 cut(s)	415, 1297, 1520
BsrGI	TGTACA	1 cut(s)	1737
BsrI	ACTGG	2 cut(s)	448, 1403
BssECI	CCNNGG	3 cut(s)	876, 1086, 1608
Bst2UI	CCWGG	2 cut(s)	1205, 1610
Bst4CI	ACNGT	9 cut(s)	634, 724, 949, 965, 1007, 1142, 1284, 1484, 1894
Bst6I	CTCTTC	1 cut(s)	646
BstAPI	GCANNNNNTGC	2 cut(s)	133, 1415
BstAUI	TGTACA	1 cut(s)	1737
BstC8I	GCNNGC	3 cut(s)	1164, 1913, 2078
BstDEI	CTNAG	4 cut(s)	992, 2032, 2064, 2090
BstF5I	GGATG	3 cut(s)	406, 589, 2116
BstHHI	GCGC	1 cut(s)	433
BstMAI	GTCTC	3 cut(s)	893, 1242, 1885
BstMCI	CGRYCG	2 cut(s)	46, 1976
BstMWI	GCNNNNNNNGC	5 cut(s)	133, 1412, 1415, 2086, 2226
BstNI	CCWGG	2 cut(s)	1205, 1610
BstNSI	RCATGY	4 cut(s)	1714, 1735, 1801, 1927
BstSCI	CCNGG	3 cut(s)	715, 1203, 1608
BstSLI	GKGCMC	2 cut(s)	1135, 1923
BstV1I	GCAGC	7 cut(s)	39, 139, 355, 863, 1291, 1402, 1418
BstX2I	RGATCY	1 cut(s)	1768
BstYI	RGATCY	1 cut(s)	1768
BstZI	CGGCCG	1 cut(s)	1973
BsuI	GTATCC	1 cut(s)	1646
BsuRI	GGCC	8 cut(s)	576, 779, 1724, 1758, 1847, 1934, 1975, 2080
BtsCI	GGATG	3 cut(s)	406, 589, 2116
BtsI	GCAGTG	1 cut(s)	1079
BtsIMutI	CAGTG	2 cut(s)	1079, 1276
Cac8I	GCNNGC	3 cut(s)	1164, 1913, 2078
CaiI	CAGNNNCTG	1 cut(s)	1415
CciI	TCATGA	1 cut(s)	2240
CfoI	GCGC	1 cut(s)	433
Cfr13I	GGNCC	2 cut(s)	1722, 1846
CsiI	ACCWGGT	1 cut(s)	1203
Csp6I	GTAC	7 cut(s)	441, 451, 630, 713, 1315, 1738, 1801
CviQI	GTAC	7 cut(s)	441, 451, 630, 713, 1315, 1738, 1801
DdeI	CTNAG	4 cut(s)	992, 2032, 2064, 2090
DraI	TTTAAA	1 cut(s)	288
DriI	GACNNNNNGTC	1 cut(s)	2155
EaeI	YGGCCR	1 cut(s)	1973
EagI	CGGCCG	1 cut(s)	1973
Eam1104I	CTCTTC	1 cut(s)	646
Eam1105I	GACNNNNNGTC	1 cut(s)	2155
EarI	CTCTTC	1 cut(s)	646
EclXI	CGGCCG	1 cut(s)	1973
Eco147I	AGGCCT	2 cut(s)	576, 779
Eco24I	GRGCYC	1 cut(s)	1609
Eco32I	GATATC	1 cut(s)	639
Eco52I	CGGCCG	1 cut(s)	1973
Eco57I	CTGAAG	1 cut(s)	1745
Eco88I	CYCGRG	1 cut(s)	1085
EcoRI	GAATTC	3 cut(s)	324, 1941, 1968
EcoRII	CCWGG	2 cut(s)	1203, 1608
EcoRV	GATATC	1 cut(s)	639
EcoT22I	ATGCAT	1 cut(s)	961
EcoT38I	GRGCYC	1 cut(s)	1609
Esp3I	CGTCTC	1 cut(s)	893
FalI	AAGNNNNNCTT	2 cut(s)	2197, 2229
FaqI	GGGAC	2 cut(s)	11, 1409
FauNDI	CATATG	1 cut(s)	1276
FbaI	TGATCA	4 cut(s)	1266, 1326, 1476, 2191
Fnu4HI	GCNGC	7 cut(s)	53, 128, 344, 852, 1305, 1407, 1416
FokI	GGATG	3 cut(s)	413, 596, 2123
FriOI	GRGCYC	1 cut(s)	1609
Fsp4HI	GCNGC	7 cut(s)	53, 128, 344, 852, 1305, 1407, 1416
FspAI	RTGCGCAY	1 cut(s)	432
FspBI	CTAG	3 cut(s)	1424, 1701, 1962
FspI	TGCGCA	1 cut(s)	432
GlaI	GCGC	1 cut(s)	432
GluI	GCNGC	7 cut(s)	53, 128, 344, 852, 1305, 1407, 1416
GsaI	CCCAGC	2 cut(s)	1070, 1560
GsuI	CTGGAG	1 cut(s)	1420
HaeIII	GGCC	8 cut(s)	576, 779, 1724, 1758, 1847, 1934, 1975, 2080
HapII	CCGG	1 cut(s)	717
HhaI	GCGC	1 cut(s)	433
Hin6I	GCGC	1 cut(s)	431
HinP1I	GCGC	1 cut(s)	431
HinfI	GANTC	8 cut(s)	259, 338, 495, 870, 1564, 1744, 2062, 2237
HpaII	CCGG	1 cut(s)	717
HphI	GGTGA	4 cut(s)	492, 646, 1187, 1978
Hpy166II	GTNNAC	3 cut(s)	621, 1201, 2160
Hpy188I	TCNGA	9 cut(s)	280, 407, 877, 1061, 1294, 1652, 1951, 2035, 2067
Hpy188III	TCNNGA	7 cut(s)	47, 63, 77, 1214, 1516, 1748, 2241
Hpy8I	GTNNAC	3 cut(s)	621, 1201, 2160
Hpy99I	CGWCG	1 cut(s)	421
HpyAV	CCTTC	4 cut(s)	434, 875, 1006, 1769
HpyCH4III	ACNGT	9 cut(s)	634, 724, 949, 965, 1007, 1142, 1284, 1484, 1894
HpyCH4IV	ACGT	1 cut(s)	270
HpyF10VI	GCNNNNNNNGC	5 cut(s)	133, 1412, 1415, 2086, 2226
HpyF3I	CTNAG	4 cut(s)	992, 2032, 2064, 2090
HpySE526I	ACGT	1 cut(s)	270
HspAI	GCGC	1 cut(s)	431
Ksp22I	TGATCA	4 cut(s)	1266, 1326, 1476, 2191
Lsp1109I	GCAGC	7 cut(s)	39, 139, 355, 863, 1291, 1402, 1418
LweI	GCATC	4 cut(s)	365, 814, 896, 1291
MabI	ACCWGGT	1 cut(s)	1203
MaeI	CTAG	3 cut(s)	1424, 1701, 1962
MaeII	ACGT	1 cut(s)	270
MboII	GAAGA	6 cut(s)	16, 398, 556, 663, 1234, 1844
MfeI	CAATTG	4 cut(s)	226, 462, 1637, 2071
MflI	RGATCY	1 cut(s)	1768
MhlI	GDGCHC	3 cut(s)	1135, 1609, 1923
MlyI	GAGTC	2 cut(s)	347, 2056
MmeI	TCCRAC	1 cut(s)	291
Mph1103I	ATGCAT	1 cut(s)	961
MroXI	GAANNNNTTC	1 cut(s)	258
MseI	TTAA	6 cut(s)	287, 293, 314, 695, 791, 2166
MslI	CAYNNNNRTG	2 cut(s)	947, 1194
MspA1I	CMGCKG	1 cut(s)	1560
MspI	CCGG	1 cut(s)	717
MspR9I	CCNGG	3 cut(s)	717, 1205, 1610
MunI	CAATTG	4 cut(s)	226, 462, 1637, 2071
Mva1269I	GAATGC	2 cut(s)	1628, 2220
MvaI	CCWGG	2 cut(s)	1205, 1610
MwoI	GCNNNNNNNGC	5 cut(s)	133, 1412, 1415, 2086, 2226
NciI	CCSGG	1 cut(s)	717
NdeI	CATATG	1 cut(s)	1276
NmuCI	GTSAC	3 cut(s)	511, 699, 1077
NsbI	TGCGCA	1 cut(s)	432
NsiI	ATGCAT	1 cut(s)	961
NspI	RCATGY	4 cut(s)	1714, 1735, 1801, 1927
OliI	CACNNNNGTG	1 cut(s)	947
PagI	TCATGA	1 cut(s)	2240
PceI	AGGCCT	2 cut(s)	576, 779
PciI	ACATGT	3 cut(s)	1710, 1731, 1797
PcsI	WCGNNNNNNNCGW	2 cut(s)	347, 356
PctI	GAATGC	2 cut(s)	1628, 2220
PdmI	GAANNNNTTC	1 cut(s)	258
PfeI	GAWTC	6 cut(s)	259, 495, 870, 1564, 1744, 2237
PflFI	GACNNNGTC	1 cut(s)	1877
PkrI	GCNGC	7 cut(s)	54, 129, 345, 853, 1306, 1408, 1417
Ple19I	CGATCG	1 cut(s)	46
PleI	GAGTC	2 cut(s)	346, 2056
PpsI	GAGTC	2 cut(s)	346, 2056
PscI	ACATGT	3 cut(s)	1710, 1731, 1797
PsiI	TTATAA	2 cut(s)	732, 1887
Psp6I	CCWGG	2 cut(s)	1203, 1608
PspFI	CCCAGC	2 cut(s)	1066, 1556
PspGI	CCWGG	2 cut(s)	1203, 1608
PspPI	GGNCC	2 cut(s)	1722, 1846
PsrI	GAACNNNNNNTAC	2 cut(s)	1637, 1669
PstNI	CAGNNNCTG	1 cut(s)	1415
PsuI	RGATCY	1 cut(s)	1768
PsyI	GACNNNGTC	1 cut(s)	1877
PvuI	CGATCG	1 cut(s)	46
PvuII	CAGCTG	1 cut(s)	1560
RsaI	GTAC	7 cut(s)	442, 452, 631, 714, 1316, 1739, 1802
RsaNI	GTAC	7 cut(s)	441, 451, 630, 713, 1315, 1738, 1801
RseI	CAYNNNNRTG	2 cut(s)	947, 1194
SaqAI	TTAA	6 cut(s)	287, 293, 314, 695, 791, 2166
SatI	GCNGC	7 cut(s)	53, 128, 344, 852, 1305, 1407, 1416
Sau96I	GGNCC	2 cut(s)	1722, 1846
SchI	GAGTC	2 cut(s)	347, 2056
ScrFI	CCNGG	3 cut(s)	717, 1205, 1610
SduI	GDGCHC	3 cut(s)	1135, 1609, 1923
SexAI	ACCWGGT	1 cut(s)	1203
SfaNI	GCATC	4 cut(s)	365, 814, 896, 1291
SmiMI	CAYNNNNRTG	2 cut(s)	947, 1194
SmlI	CTYRAG	1 cut(s)	781
SmoI	CTYRAG	1 cut(s)	781
SseBI	AGGCCT	2 cut(s)	576, 779
SsiI	CCGC	1 cut(s)	2105
SspMI	CTAG	3 cut(s)	1424, 1701, 1962
StuI	AGGCCT	2 cut(s)	576, 779
StyD4I	CCNGG	3 cut(s)	715, 1203, 1608
TaaI	ACNGT	9 cut(s)	634, 724, 949, 965, 1007, 1142, 1284, 1484, 1894
TaiI	ACGT	1 cut(s)	273
TaqI	TCGA	8 cut(s)	36, 46, 78, 359, 553, 1213, 1515, 2183
TatI	WGTACW	1 cut(s)	1737
TfiI	GAWTC	6 cut(s)	259, 495, 870, 1564, 1744, 2237
Tru1I	TTAA	6 cut(s)	287, 293, 314, 695, 791, 2166
Tru9I	TTAA	6 cut(s)	287, 293, 314, 695, 791, 2166
TscAI	CASTG	2 cut(s)	1086, 1276
TseFI	GTSAC	3 cut(s)	511, 699, 1077
TseI	GCWGC	7 cut(s)	52, 127, 343, 851, 1304, 1406, 1415
Tsp45I	GTSAC	3 cut(s)	511, 699, 1077
TspRI	CASTG	2 cut(s)	1086, 1276
Tth111I	GACNNNGTC	1 cut(s)	1877
XapI	RAATTY	9 cut(s)	31, 283, 324, 608, 1533, 1591, 1855, 1941, 1968
XceI	RCATGY	4 cut(s)	1714, 1735, 1801, 1927
XmnI	GAANNNNTTC	1 cut(s)	258
XspI	CTAG	3 cut(s)	1424, 1701, 1962
Zsp2I	ATGCAT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.