MD13G1252900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015817
Physical Location & Seq
Reverse (-)
27141496 .. 27142101
606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1252900.v1.1.491

Sequence Viewer

Length: 606 bp
ATGGGTTATGAACCTAGAAGGAAAATGGAAACGAAGGGAGTTTCGGCGCTTGGAGGGGATCAAGAGTTTTTGTTCAACAGAAACCTCTCAAGAAACTCCTCATCCTCATTGGGTTGCTCTTCTAGGGTTTATTACTGTAGGAGCACTGAAGGGGTTCCATTTAACTGGGAAATGCAGCCAGGAACACCGCGGAACCCTCCGAAAGAAGAAGTGATTCCACCGATAAGTCCTCCCCCGGCTGTGCTCAGCTTAGGGCTACCCAACCCCTGCATTGATCAACATCCTCGTAGTAATAAGCCTCCAACAAGCCCTCTGCATAGGCTCAAGTTTTGGACAAAAAAAAGTAAGAAAAATAAACAACGTAAGAATAATTTTCAACACAACGTTAATGTTGTTGGTGAGTCAGATAAGTTTGATAAGGTCGAGTTTTGCAGCTCTGACTCTGAGTTCATGGCATCATCAACTTCGCCTCGGAACTCAAGCTCTTCATTTTCATCCTCGTTTTCCTTTTCGAAAGGGCAGTGCGCGAGTTTAAGGAGTACTCCGGCAAGGGATTTGTTCACTAGTTCTGGTCATTTCAGCTGCAGTCCTTGGAAATTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.44

Weight (kDa)

9.69

Isoelectric Point (pI)

62.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015817)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25845 AT5G57400 AT5G57400
malus_domestica MD13G1252900.v1.1 MD16G1257400.v1.1
prunus_persica Prupe.1G085200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0402361
rosa_laevigata RLG00000009100
rosa_multiflora Rmu_sc0002324.1_g000011
rosa_roxburghii Rroxscaffold_5G00347020
rosa_samantha Rh4AG112200 Rh4BG104800 Rh4CG119000 Rh4DG103700
rosa_wichuraiana Rw4G008990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 190, 527
AciI CCGC 2 cut(s) 188, 190
AclI AACGTT 1 cut(s) 384
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 1 cut(s) 541
AgsI TTSAA 2 cut(s) 76, 377
AhlI ACTAGT 1 cut(s) 563
AjnI CCWGG 1 cut(s) 178
AluBI AGCT 5 cut(s) 249, 435, 483, 582, 603
AluI AGCT 5 cut(s) 249, 435, 483, 582, 603
Alw21I GWGCWC 2 cut(s) 146, 246
AlwI GGATC 1 cut(s) 66
ApeKI GCWGC 3 cut(s) 175, 432, 582
ApoI RAATTY 1 cut(s) 596
Asp700I GAANNNNTTC 2 cut(s) 153, 213
AspLEI GCGC 2 cut(s) 49, 527
AsuC2I CCSGG 1 cut(s) 236
AsuHPI GGTGA 1 cut(s) 410
AsuII TTCGAA 1 cut(s) 512
Bbv12I GWGCWC 2 cut(s) 146, 246
BbvI GCAGC 3 cut(s) 187, 444, 569
BciT130I CCWGG 1 cut(s) 180
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 1 cut(s) 236
BcuI ACTAGT 1 cut(s) 563
BfaI CTAG 4 cut(s) 15, 123, 564, 604
BfmI CTRYAG 2 cut(s) 136, 583
BfoI RGCGCY 1 cut(s) 50
BisI GCNGC 3 cut(s) 176, 433, 583
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 3 cut(s) 177, 434, 584
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 180, 236
BmiI GGNNCC 2 cut(s) 156, 194
BmrFI CCNGG 2 cut(s) 180, 236
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 250
Bpu1102I GCTNAGC 1 cut(s) 245
Bpu14I TTCGAA 1 cut(s) 512
BpuEI CTTGAG 3 cut(s) 73, 308, 463
BpuMI CCSGG 1 cut(s) 236
BsaBI GATNNNNATC 1 cut(s) 279
BsaJI CCNNGG 4 cut(s) 188, 234, 470, 590
Bse1I ACTGG 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 4 cut(s) 188, 234, 470, 590
BseGI GGATG 3 cut(s) 101, 280, 494
BseJI GATNNNNATC 1 cut(s) 279
BseMII CTCAG 2 cut(s) 259, 435
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 1 cut(s) 88
BseXI GCAGC 3 cut(s) 187, 444, 569
Bsh1236I CGCG 2 cut(s) 190, 527
BsiHKAI GWGCWC 2 cut(s) 146, 246
BsiSI CCGG 2 cut(s) 236, 545
Bsp119I TTCGAA 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 146, 246
Bsp143I GATC 2 cut(s) 58, 274
Bsp1720I GCTNAGC 1 cut(s) 245
BspACI CCGC 2 cut(s) 188, 190
BspCNI CTCAG 2 cut(s) 258, 436
BspFNI CGCG 2 cut(s) 190, 527
BspLI GGNNCC 2 cut(s) 156, 194
BspMAI CTGCAG 1 cut(s) 587
BspPI GGATC 1 cut(s) 66
BspQI GCTCTTC 2 cut(s) 124, 490
BspT104I TTCGAA 1 cut(s) 512
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 4 cut(s) 188, 234, 470, 590
BssMI GATC 2 cut(s) 58, 274
BssT1I CCWWGG 1 cut(s) 590
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 137
Bst6I CTCTTC 2 cut(s) 124, 490
BstBI TTCGAA 1 cut(s) 512
BstDEI CTNAG 3 cut(s) 245, 250, 444
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 3 cut(s) 101, 280, 494
BstFNI CGCG 2 cut(s) 190, 527
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 2 cut(s) 49, 527
BstKTI GATC 2 cut(s) 61, 277
BstMBI GATC 2 cut(s) 58, 274
BstNI CCWGG 1 cut(s) 180
BstSCI CCNGG 2 cut(s) 178, 234
BstSFI CTRYAG 2 cut(s) 136, 583
BstUI CGCG 2 cut(s) 190, 527
BstV1I GCAGC 3 cut(s) 187, 444, 569
BstXI CCANNNNNNTGG 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 3 cut(s) 101, 280, 494
BtsI GCAGTG 1 cut(s) 527
BtsIMutI CAGTG 2 cut(s) 144, 527
CfoI GCGC 2 cut(s) 49, 527
Cfr42I CCGCGG 1 cut(s) 191
Csp6I GTAC 1 cut(s) 540
CviAII CATG 1 cut(s) 451
CviQI GTAC 1 cut(s) 540
DdeI CTNAG 3 cut(s) 245, 250, 444
DpnI GATC 2 cut(s) 60, 276
DpnII GATC 2 cut(s) 58, 274
Eam1104I CTCTTC 2 cut(s) 124, 490
EarI CTCTTC 2 cut(s) 124, 490
Eco130I CCWWGG 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 168
EcoRII CCWGG 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 590
FaeI CATG 1 cut(s) 454
FaiI YATR 3 cut(s) 9, 318, 452
FatI CATG 1 cut(s) 450
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 3 cut(s) 176, 433, 583
FokI GGATG 3 cut(s) 88, 267, 481
Fsp4HI GCNGC 3 cut(s) 176, 433, 583
FspBI CTAG 4 cut(s) 15, 123, 564, 604
GlaI GCGC 2 cut(s) 48, 526
GluI GCNGC 3 cut(s) 176, 433, 583
HaeII RGCGCY 1 cut(s) 50
HapII CCGG 2 cut(s) 236, 545
HhaI GCGC 2 cut(s) 49, 527
Hin1II CATG 1 cut(s) 454
Hin6I GCGC 2 cut(s) 47, 525
HinP1I GCGC 2 cut(s) 47, 525
HinfI GANTC 3 cut(s) 214, 401, 440
HpaII CCGG 2 cut(s) 236, 545
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 1 cut(s) 561
Hpy188I TCNGA 5 cut(s) 201, 406, 439, 445, 474
Hpy188III TCNNGA 2 cut(s) 62, 90
Hpy8I GTNNAC 1 cut(s) 561
HpyAV CCTTC 3 cut(s) 12, 28, 143
HpyCH4III ACNGT 1 cut(s) 137
HpyCH4IV ACGT 2 cut(s) 361, 384
HpyCH4V TGCA 5 cut(s) 175, 270, 316, 432, 585
HpyF3I CTNAG 3 cut(s) 245, 250, 444
HpySE526I ACGT 2 cut(s) 361, 384
Hsp92II CATG 1 cut(s) 454
HspAI GCGC 2 cut(s) 47, 525
Ksp22I TGATCA 1 cut(s) 274
KspI CCGCGG 1 cut(s) 191
Kzo9I GATC 2 cut(s) 58, 274
LguI GCTCTTC 2 cut(s) 124, 490
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 7 cut(s) 151, 165, 192, 249, 280, 555, 558
Lsp1109I GCAGC 3 cut(s) 187, 444, 569
LweI GCATC 1 cut(s) 464
MaeI CTAG 4 cut(s) 15, 123, 564, 604
MaeII ACGT 2 cut(s) 361, 384
MalI GATC 2 cut(s) 60, 276
MboI GATC 2 cut(s) 58, 274
MboII GAAGA 3 cut(s) 111, 218, 477
MhlI GDGCHC 2 cut(s) 146, 246
MluCI AATT 2 cut(s) 370, 596
MlyI GAGTC 2 cut(s) 410, 434
MmeI TCCRAC 1 cut(s) 326
MroXI GAANNNNTTC 2 cut(s) 153, 213
MseI TTAA 3 cut(s) 162, 387, 533
MspA1I CMGCKG 2 cut(s) 190, 582
MspI CCGG 2 cut(s) 236, 545
MspR9I CCNGG 2 cut(s) 180, 236
MvaI CCWGG 1 cut(s) 180
MvnI CGCG 2 cut(s) 190, 527
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 58, 274
NlaIII CATG 1 cut(s) 454
NlaIV GGNNCC 2 cut(s) 156, 194
NspV TTCGAA 1 cut(s) 512
PciSI GCTCTTC 2 cut(s) 124, 490
PdmI GAANNNNTTC 2 cut(s) 153, 213
PfeI GAWTC 1 cut(s) 214
PkrI GCNGC 3 cut(s) 177, 434, 584
PleI GAGTC 2 cut(s) 409, 434
PpsI GAGTC 2 cut(s) 409, 434
Psp1406I AACGTT 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 156, 194
PstI CTGCAG 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 582
RsaI GTAC 1 cut(s) 541
RsaNI GTAC 1 cut(s) 540
SacII CCGCGG 1 cut(s) 191
SapI GCTCTTC 2 cut(s) 124, 490
SaqAI TTAA 3 cut(s) 162, 387, 533
SatI GCNGC 3 cut(s) 176, 433, 583
Sau3AI GATC 2 cut(s) 58, 274
ScaI AGTACT 1 cut(s) 541
SchI GAGTC 2 cut(s) 410, 434
ScrFI CCNGG 2 cut(s) 180, 236
SduI GDGCHC 2 cut(s) 146, 246
SfaNI GCATC 1 cut(s) 464
SfcI CTRYAG 2 cut(s) 136, 583
Sfr303I CCGCGG 1 cut(s) 191
SfuI TTCGAA 1 cut(s) 512
SgrBI CCGCGG 1 cut(s) 191
SmlI CTYRAG 3 cut(s) 88, 323, 478
SmoI CTYRAG 3 cut(s) 88, 323, 478
SpeI ACTAGT 1 cut(s) 563
Sse9I AATT 2 cut(s) 370, 596
SsiI CCGC 2 cut(s) 188, 190
SspMI CTAG 4 cut(s) 15, 123, 564, 604
StyD4I CCNGG 2 cut(s) 178, 234
StyI CCWWGG 1 cut(s) 590
TaaI ACNGT 1 cut(s) 137
TaiI ACGT 2 cut(s) 364, 387
TaqI TCGA 2 cut(s) 423, 512
TasI AATT 2 cut(s) 370, 596
TatI WGTACW 1 cut(s) 539
TfiI GAWTC 1 cut(s) 214
Tru1I TTAA 3 cut(s) 162, 387, 533
Tru9I TTAA 3 cut(s) 162, 387, 533
TscAI CASTG 2 cut(s) 151, 527
TseI GCWGC 3 cut(s) 175, 432, 582
TspDTI ATGAA 4 cut(s) 24, 439, 477, 483
TspRI CASTG 2 cut(s) 151, 527
XapI RAATTY 1 cut(s) 596
XmnI GAANNNNTTC 2 cut(s) 153, 213
XspI CTAG 4 cut(s) 15, 123, 564, 604
ZrmI AGTACT 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.