MD16G1257400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015817
Physical Location & Seq
Reverse (-)
30158408 .. 30159031
624 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1257400.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGGTTCTGAGCCTCGAAGGAAAATGGAAACGAAAGGAGTTTCGGTACTTGGAGGGGATCAAGAATTTTTGTTCAACAGAAACCTCTTAAGAAACTCCTCATCCCCATTGGGTTGCTCTTCTAGGGTTTATTACTGCAGGAGCACTGAAGGGGTTCCATTTGACTGGGAAATGCAGCCAGGAACACCACGGAACCCGCCAAAAGAAGAAGCAATTCCACCAATAAGCCCTCCCCCGGCTGTGCTCAGCTTAGGGCTACCCAAGCCCTGCATTGATCAGCAGCTTCATACTAATAAGCCTCCAACAAGCCCTCTGCATAGGGTCAAGTTTTGGAGTAAAAAAAGTAAGACAAAAAAACAACGTAAGAACAATTTGCAACACAATGGTGGCTTAGATAAGTTTGATAAGGTCGAGTTTTGCAGCTCTGATTCTGAGTTCATGGCATCGTCAAGTTCGCCTCGGAACTCAAGCTCTTCATCTTCATCTTCATTTTCTTTTTCGAAAGGGCAGTGCTCGAGTTTAAGAAGTACTCCGGCAAGGGATTCTTTCACTAGTTCTGGTCATTTCAGCTGCAGTCCTTGGAAATTAAGCTCTTTTCTCACTAGCGCTGCAAGGCGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

22.83

Weight (kDa)

9.87

Isoelectric Point (pI)

73.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015817)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25845 AT5G57400 AT5G57400
malus_domestica MD13G1252900.v1.1 MD16G1257400.v1.1
prunus_persica Prupe.1G085200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0402361
rosa_laevigata RLG00000009100
rosa_multiflora Rmu_sc0002324.1_g000011
rosa_roxburghii Rroxscaffold_5G00347020
rosa_samantha Rh4AG112200 Rh4BG104800 Rh4CG119000 Rh4DG103700
rosa_wichuraiana Rw4G008990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 197
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 168
AfaI GTAC 2 cut(s) 48, 529
AfeI AGCGCT 1 cut(s) 607
AfiI CCNNNNNNNGG 1 cut(s) 235
AflII CTTAAG 1 cut(s) 88
AgsI TTSAA 1 cut(s) 76
AhlI ACTAGT 1 cut(s) 551
AjnI CCWGG 1 cut(s) 178
AleI CACNNNNGTG 1 cut(s) 384
AluBI AGCT 7 cut(s) 249, 283, 423, 471, 570, 591, 620
AluI AGCT 7 cut(s) 249, 283, 423, 471, 570, 591, 620
Alw21I GWGCWC 3 cut(s) 146, 246, 515
AlwI GGATC 1 cut(s) 66
Ama87I CYCGRG 1 cut(s) 514
Aor51HI AGCGCT 1 cut(s) 607
ApeKI GCWGC 5 cut(s) 175, 280, 420, 570, 608
ApoI RAATTY 1 cut(s) 65
Asp700I GAANNNNTTC 2 cut(s) 153, 213
AspLEI GCGC 1 cut(s) 608
AsuC2I CCSGG 1 cut(s) 236
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 1 cut(s) 514
Bbv12I GWGCWC 3 cut(s) 146, 246, 515
BbvI GCAGC 5 cut(s) 187, 292, 432, 557, 595
BciT130I CCWGG 1 cut(s) 180
BclI TGATCA 1 cut(s) 274
BcnI CCSGG 1 cut(s) 236
BcuI ACTAGT 1 cut(s) 551
BfaI CTAG 3 cut(s) 123, 552, 603
BfmI CTRYAG 2 cut(s) 136, 571
BfoI RGCGCY 1 cut(s) 609
BfrI CTTAAG 1 cut(s) 88
BisI GCNGC 5 cut(s) 176, 281, 421, 571, 609
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 5 cut(s) 177, 282, 422, 572, 610
BmcAI AGTACT 1 cut(s) 529
Bme1390I CCNGG 2 cut(s) 180, 236
BmeT110I CYCGRG 1 cut(s) 514
BmiI GGNNCC 2 cut(s) 156, 194
BmrFI CCNGG 2 cut(s) 180, 236
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 1 cut(s) 452
BmuI ACTGGG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 250
Bpu1102I GCTNAGC 1 cut(s) 245
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 451
BpuMI CCSGG 1 cut(s) 236
BsaJI CCNNGG 4 cut(s) 188, 234, 458, 578
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 235
Bse1I ACTGG 1 cut(s) 170
BseBI CCWGG 1 cut(s) 180
BseDI CCNNGG 4 cut(s) 188, 234, 458, 578
BseGI GGATG 1 cut(s) 101
BseLI CCNNNNNNNGG 1 cut(s) 235
BseMII CTCAG 2 cut(s) 259, 423
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 1 cut(s) 88
BseXI GCAGC 5 cut(s) 187, 292, 432, 557, 595
BsiHKAI GWGCWC 3 cut(s) 146, 246, 515
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 2 cut(s) 236, 533
BslI CCNNNNNNNGG 1 cut(s) 235
BsoBI CYCGRG 1 cut(s) 514
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 3 cut(s) 146, 246, 515
Bsp143I GATC 2 cut(s) 58, 274
Bsp1720I GCTNAGC 1 cut(s) 245
BspACI CCGC 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 258, 424
BspLI GGNNCC 2 cut(s) 156, 194
BspMAI CTGCAG 2 cut(s) 140, 575
BspPI GGATC 1 cut(s) 66
BspQI GCTCTTC 2 cut(s) 124, 478
BspT104I TTCGAA 1 cut(s) 500
BspTI CTTAAG 1 cut(s) 88
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 4 cut(s) 188, 234, 458, 578
BssMI GATC 2 cut(s) 58, 274
BssT1I CCWWGG 1 cut(s) 578
Bst2UI CCWGG 1 cut(s) 180
Bst6I CTCTTC 2 cut(s) 124, 478
BstAFI CTTAAG 1 cut(s) 88
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 5 cut(s) 9, 245, 250, 391, 432
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 1 cut(s) 101
BstH2I RGCGCY 1 cut(s) 609
BstHHI GCGC 1 cut(s) 608
BstKTI GATC 2 cut(s) 61, 277
BstMBI GATC 2 cut(s) 58, 274
BstMWI GCNNNNNNNGC 2 cut(s) 262, 617
BstNI CCWGG 1 cut(s) 180
BstSCI CCNGG 2 cut(s) 178, 234
BstSFI CTRYAG 2 cut(s) 136, 571
BstV1I GCAGC 5 cut(s) 187, 292, 432, 557, 595
BstXI CCANNNNNNTGG 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 1 cut(s) 101
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 2 cut(s) 144, 515
Cac8I GCNNGC 1 cut(s) 618
CfoI GCGC 1 cut(s) 608
Csp6I GTAC 2 cut(s) 47, 528
CviAII CATG 1 cut(s) 439
CviQI GTAC 2 cut(s) 47, 528
DdeI CTNAG 5 cut(s) 9, 245, 250, 391, 432
DpnI GATC 2 cut(s) 60, 276
DpnII GATC 2 cut(s) 58, 274
Eam1104I CTCTTC 2 cut(s) 124, 478
EarI CTCTTC 2 cut(s) 124, 478
Eco130I CCWWGG 1 cut(s) 578
Eco47III AGCGCT 1 cut(s) 607
Eco57I CTGAAG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 578
FaeI CATG 1 cut(s) 442
FaiI YATR 3 cut(s) 288, 318, 440
FalI AAGNNNNNCTT 3 cut(s) 529, 561, 604
FatI CATG 1 cut(s) 438
FauI CCCGC 1 cut(s) 204
FbaI TGATCA 1 cut(s) 274
Fnu4HI GCNGC 5 cut(s) 176, 281, 421, 571, 609
FokI GGATG 1 cut(s) 88
Fsp4HI GCNGC 5 cut(s) 176, 281, 421, 571, 609
FspBI CTAG 3 cut(s) 123, 552, 603
GlaI GCGC 1 cut(s) 607
GluI GCNGC 5 cut(s) 176, 281, 421, 571, 609
HaeII RGCGCY 1 cut(s) 609
HapII CCGG 2 cut(s) 236, 533
HhaI GCGC 1 cut(s) 608
Hin1II CATG 1 cut(s) 442
Hin6I GCGC 1 cut(s) 606
HinP1I GCGC 1 cut(s) 606
HinfI GANTC 2 cut(s) 428, 542
HpaII CCGG 2 cut(s) 236, 533
Hpy188I TCNGA 4 cut(s) 10, 427, 433, 462
Hpy188III TCNNGA 1 cut(s) 62
HpyAV CCTTC 2 cut(s) 12, 143
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 8 cut(s) 138, 175, 270, 316, 376, 420, 573, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 617
HpyF3I CTNAG 5 cut(s) 9, 245, 250, 391, 432
HpySE526I ACGT 1 cut(s) 361
Hsp92II CATG 1 cut(s) 442
HspAI GCGC 1 cut(s) 606
Ksp22I TGATCA 1 cut(s) 274
Kzo9I GATC 2 cut(s) 58, 274
LguI GCTCTTC 2 cut(s) 124, 478
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 8 cut(s) 124, 151, 165, 192, 249, 280, 543, 546
Lsp1109I GCAGC 5 cut(s) 187, 292, 432, 557, 595
LweI GCATC 1 cut(s) 452
MaeI CTAG 3 cut(s) 123, 552, 603
MaeII ACGT 1 cut(s) 361
MalI GATC 2 cut(s) 60, 276
MboI GATC 2 cut(s) 58, 274
MboII GAAGA 5 cut(s) 111, 218, 465, 471, 477
MhlI GDGCHC 3 cut(s) 146, 246, 515
MluCI AATT 4 cut(s) 65, 213, 370, 584
MmeI TCCRAC 1 cut(s) 326
MnlI CCTC 8 cut(s) 24, 47, 95, 109, 240, 309, 321, 468
MroXI GAANNNNTTC 2 cut(s) 153, 213
MseI TTAA 3 cut(s) 89, 521, 587
MslI CAYNNNNRTG 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 570
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 2 cut(s) 236, 533
MspR9I CCNGG 2 cut(s) 180, 236
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 262, 617
NciI CCSGG 1 cut(s) 236
NdeII GATC 2 cut(s) 58, 274
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 2 cut(s) 156, 194
NspV TTCGAA 1 cut(s) 500
OliI CACNNNNGTG 1 cut(s) 384
PaeR7I CTCGAG 1 cut(s) 514
PciSI GCTCTTC 2 cut(s) 124, 478
PdmI GAANNNNTTC 2 cut(s) 153, 213
PfeI GAWTC 2 cut(s) 428, 542
PkrI GCNGC 5 cut(s) 177, 282, 422, 572, 610
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 156, 194
PspXI VCTCGAGB 1 cut(s) 514
PstI CTGCAG 2 cut(s) 140, 575
PvuII CAGCTG 1 cut(s) 570
RsaI GTAC 2 cut(s) 48, 529
RsaNI GTAC 2 cut(s) 47, 528
RseI CAYNNNNRTG 1 cut(s) 384
SapI GCTCTTC 2 cut(s) 124, 478
SaqAI TTAA 3 cut(s) 89, 521, 587
SatI GCNGC 5 cut(s) 176, 281, 421, 571, 609
Sau3AI GATC 2 cut(s) 58, 274
ScaI AGTACT 1 cut(s) 529
ScrFI CCNGG 2 cut(s) 180, 236
SduI GDGCHC 3 cut(s) 146, 246, 515
SfaNI GCATC 1 cut(s) 452
SfcI CTRYAG 2 cut(s) 136, 571
Sfr274I CTCGAG 1 cut(s) 514
SfuI TTCGAA 1 cut(s) 500
SlaI CTCGAG 1 cut(s) 514
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 3 cut(s) 88, 466, 514
SmoI CTYRAG 3 cut(s) 88, 466, 514
SpeI ACTAGT 1 cut(s) 551
Sse9I AATT 4 cut(s) 65, 213, 370, 584
SsiI CCGC 1 cut(s) 197
SspMI CTAG 3 cut(s) 123, 552, 603
StyD4I CCNGG 2 cut(s) 178, 234
StyI CCWWGG 1 cut(s) 578
TaiI ACGT 1 cut(s) 364
TaqI TCGA 4 cut(s) 16, 411, 500, 515
TasI AATT 4 cut(s) 65, 213, 370, 584
TatI WGTACW 1 cut(s) 527
TfiI GAWTC 2 cut(s) 428, 542
Tru1I TTAA 3 cut(s) 89, 521, 587
Tru9I TTAA 3 cut(s) 89, 521, 587
TscAI CASTG 2 cut(s) 151, 515
TseI GCWGC 5 cut(s) 175, 280, 420, 570, 608
TspDTI ATGAA 5 cut(s) 275, 427, 465, 471, 477
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 2 cut(s) 151, 515
Vha464I CTTAAG 1 cut(s) 88
XapI RAATTY 1 cut(s) 65
XhoI CTCGAG 1 cut(s) 514
XmnI GAANNNNTTC 2 cut(s) 153, 213
XspI CTAG 3 cut(s) 123, 552, 603
ZrmI AGTACT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.