MD14G1032500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011439
Physical Location & Seq
Reverse (-)
2926309 .. 2926500
192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1032500.v1.1.491

Sequence Viewer

Length: 192 bp
ATGGTGGTGTGGTCCTACCCACCGACGCGGAAGCAGCTGGCCGTGACGGCGTTCTGTTTCGTAACGGGGGTGGCGCTCTTCGCCATTGGAGCTCACCTATCGCTGGCAAACGTGGGCCCACAACAAGACCGGGTCAAGGCTCGAAGGGACTTCGTCAAAGACCGACTCAGAAAGCTCCTCGATGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

64

Amino Acids

7.1

Weight (kDa)

9.85

Isoelectric Point (pI)

25.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 28
AciI CCGC 1 cut(s) 28
AcoI YGGCCR 1 cut(s) 39
AfiI CCNNNNNNNGG 2 cut(s) 103, 136
AluBI AGCT 3 cut(s) 37, 92, 175
AluI AGCT 3 cut(s) 37, 92, 175
Alw21I GWGCWC 1 cut(s) 94
AoxI GGCC 2 cut(s) 39, 115
ApaI GGGCCC 1 cut(s) 119
ApeKI GCWGC 1 cut(s) 34
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 3 cut(s) 12, 115, 116
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 86
AvaII GGWCC 1 cut(s) 12
BaeGI GKGCMC 1 cut(s) 119
BanII GRGCYC 2 cut(s) 94, 119
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 26, 63
BcnI CCSGG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 77
BglI GCCNNNNNGGC 1 cut(s) 47
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
Bme1390I CCNGG 1 cut(s) 131
Bme18I GGWCC 1 cut(s) 12
BmgT120I GGNCC 3 cut(s) 12, 115, 116
BmiI GGNNCC 1 cut(s) 117
BmrFI CCNGG 1 cut(s) 131
BpuMI CCSGG 1 cut(s) 131
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 136
BseLI CCNNNNNNNGG 2 cut(s) 103, 136
BseMII CTCAG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 167
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 1 cut(s) 46
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 41, 117
BsiHKAI GWGCWC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 130
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 2 cut(s) 103, 136
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 2 cut(s) 41, 117
Bsp120I GGGCCC 1 cut(s) 115
Bsp1286I GDGCHC 2 cut(s) 94, 119
BspACI CCGC 1 cut(s) 28
BspANI GGCC 2 cut(s) 41, 117
BspCNI CTCAG 1 cut(s) 180
BspFNI CGCG 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 117
BspQI GCTCTTC 1 cut(s) 83
Bst6I CTCTTC 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 39, 105
BstDEI CTNAG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 28
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstMWI GCNNNNNNNGC 4 cut(s) 34, 47, 80, 89
BstSCI CCNGG 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 46
BsuRI GGCC 2 cut(s) 41, 117
Cac8I GCNNGC 2 cut(s) 39, 105
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 3 cut(s) 12, 115, 116
CseI GACGC 1 cut(s) 34
CviJI RGCY 6 cut(s) 37, 41, 92, 117, 140, 175
CviKI_1 RGCY 6 cut(s) 37, 41, 92, 117, 140, 175
DdeI CTNAG 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 92
Eco24I GRGCYC 2 cut(s) 94, 119
Eco47I GGWCC 1 cut(s) 12
Eco53kI GAGCTC 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 92
EcoT38I GRGCYC 2 cut(s) 94, 119
FaqI GGGAC 1 cut(s) 161
Fnu4HI GCNGC 1 cut(s) 35
FriOI GRGCYC 2 cut(s) 94, 119
Fsp4HI GCNGC 1 cut(s) 35
GlaI GCGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 35
HaeII RGCGCY 1 cut(s) 77
HaeIII GGCC 2 cut(s) 41, 117
HapII CCGG 1 cut(s) 130
HgaI GACGC 1 cut(s) 34
HhaI GCGC 1 cut(s) 76
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 1 cut(s) 165
HpaII CCGG 1 cut(s) 130
HphI GGTGA 1 cut(s) 86
Hpy188I TCNGA 1 cut(s) 170
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 138
HpyCH4IV ACGT 1 cut(s) 111
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 47, 80, 89
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 111
HspAI GCGC 1 cut(s) 74
LguI GCTCTTC 1 cut(s) 83
LmnI GCTCC 2 cut(s) 89, 180
LpnPI CCDG 3 cut(s) 23, 89, 143
Lsp1109I GCAGC 1 cut(s) 46
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 2 cut(s) 43, 61
MboII GAAGA 1 cut(s) 70
MhlI GDGCHC 2 cut(s) 94, 119
MlyI GAGTC 1 cut(s) 159
MnlI CCTC 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 1 cut(s) 130
MspR9I CCNGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 4 cut(s) 34, 47, 80, 89
NciI CCSGG 1 cut(s) 131
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 1 cut(s) 43
PciSI GCTCTTC 1 cut(s) 83
PflFI GACNNNGTC 2 cut(s) 131, 152
PkrI GCNGC 1 cut(s) 36
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 117
PspOMI GGGCCC 1 cut(s) 115
PspPI GGNCC 3 cut(s) 12, 115, 116
PsyI GACNNNGTC 2 cut(s) 131, 152
PvuII CAGCTG 1 cut(s) 37
SacI GAGCTC 1 cut(s) 94
SapI GCTCTTC 1 cut(s) 83
SatI GCNGC 1 cut(s) 35
Sau96I GGNCC 3 cut(s) 12, 115, 116
SchI GAGTC 1 cut(s) 159
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 2 cut(s) 94, 119
SetI ASST 5 cut(s) 39, 94, 99, 114, 177
SinI GGWCC 1 cut(s) 12
SsiI CCGC 1 cut(s) 28
SstI GAGCTC 1 cut(s) 94
StyD4I CCNGG 1 cut(s) 129
TaiI ACGT 1 cut(s) 114
TaqI TCGA 2 cut(s) 142, 180
TaqII GACCGA 1 cut(s) 177
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 43
Tth111I GACNNNGTC 2 cut(s) 131, 152
VpaK11BI GGWCC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.