MD14G1079600.v1.1

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012790
Physical Location & Seq
Reverse (-)
9082994 .. 9085616
2623 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1079600.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGGCAAAGACAAAGTGCTGGTTTGGTTGGGTCAAGAAGCTCTTCACTTCTGAAGCAAACTCAAAGACACAAAAGAAATCGAAGAACTGGAAATGGATTTTCGGGAAGTTGGAGGTACTTCCTCAGTACCCTGCACAGCCTGCACTGATCACAGCATCATCATCAGAGAGAGCAGTACTGCTCAGTGAAGCAACAGAAGAGCAGAAAAGACATGCTTTCACTGTTGCAATTGCAACCGCTGCTGCAGCAGAGGCAGCAGTAGCTGCTGCCCGTGCTGCAGCTGAGGTTGTGCGCCTTACTACTACTTCAAAATCTCGCCACTATCACACAAAGTTTGATAGGAACTTGGCCGCCATTAAAATTCAAAGCGTTTATCGCGCACATCTTGCAAGGAAAGCATTGCGAGCGTTGAAAGGACTCGTACGAGTTCAAGCAATTGTTCGTGGCAGAGCTGTGAGACGACGACAAGCAGCTACCACTTTGAAGCGTTTGCCGTCCAACAGAAAAGGAAATGAGGGGCAGGCAGAGGTTTGCCAAAGGAGCATTCTTACTACACACGAGATATGTAGTGATGGTGAAAACAAACAGCTGATCAGCAAGACAAAAAGGGAGTGGGAAGAAAATGAAACACCACTTCAATGTAGCAGCCAGAGAGGGTGGGATTTCAGTATAATTTCACAGGAAGACATGGAATCCGTACGGCTAAGAAAGCAAGAGGCTATGACCAAAAGAGATAGGATGAAGAAATACTCTTTTTCTCTACGGGAAAGCAGAAATGTGCAAATGTTAGAAGATTCAGCGAGCATCAACGAAACTGGAACGCTAATCCTTCATTCAAATCTTAGTGCTAGTACTGGAGCATTGTTTGGGAGATCACAAGTTAAACCGCAACATGTGAGAAATGAAGATTCAGTGGAAAGACCGACCCCAAAATTTCTGGTATTGCCAAGGAGATCATTTTGCAATGTAGAAGCGGAACAGAACAATTCTGTTCATGATCAACATTACATGGCTGTGACAGCATCTGCCAAGGCAAAGACACGGTCGCTAAGTACCCCGAAGCACCGCCTAGAATCCCAGGATCGACACAACTATGGGATTTCTCTTTCTTCTTCTTATGATGCTGAGTCTGTTAGCAAGAATAGCAAGAATGGATGGGGTTCTTTGGCAGATATGTTGAAGCAACATCACCATAAACAACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

402

Amino Acids

45.29

Weight (kDa)

10.14

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 117 - 135 1e-05 IQ calmodulin-binding motif
DUF4005 PF13178 332 - 393 2.9e-09 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76705 AT5G03960 AT5G03960 AT5G03960
fragaria_vesca FvH4_6g24720
malus_domestica MD14G1079600.v1.1
prunus_persica Prupe.7G050600_v2.0.a1
pyrus_communis pycom12g07200 pycom12g07210
rosa_chinensis RchiOBHm_Chr3g0484271
rosa_laevigata RLG00000023235
rosa_rugosa Rorug03G0210500 Rorug03G0210500
rosa_samantha Rh3AG259500 Rh3BG296100 Rh3CG293600 Rh3DG289500
rosa_wichuraiana Rw3G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 378
AciI CCGC 5 cut(s) 237, 351, 887, 974, 1066
AclI AACGTT 1 cut(s) 1201
AclWI GGATC 1 cut(s) 1089
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 2 cut(s) 360, 932
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 7 cut(s) 117, 128, 177, 423, 699, 853, 1054
AflIII ACRYGT 1 cut(s) 892
AgsI TTSAA 8 cut(s) 309, 365, 412, 431, 484, 638, 837, 1180
AjnI CCWGG 1 cut(s) 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 528, 560
AluBI AGCT 6 cut(s) 40, 263, 281, 452, 473, 589
AluI AGCT 6 cut(s) 40, 263, 281, 452, 473, 589
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 1 cut(s) 1089
AlwNI CAGNNNCTG 2 cut(s) 263, 1025
AoxI GGCC 1 cut(s) 348
ApoI RAATTY 2 cut(s) 360, 932
ArsI GACNNNNNNTTYG 2 cut(s) 1028, 1060
Asp700I GAANNNNTTC 1 cut(s) 41
AspLEI GCGC 2 cut(s) 294, 380
AsuHPI GGTGA 2 cut(s) 587, 1181
BauI CACGAG 1 cut(s) 557
BbsI GAAGAC 1 cut(s) 690
BbvCI CCTCAGC 1 cut(s) 282
BccI CCATC 2 cut(s) 566, 1149
BceAI ACGGC 2 cut(s) 478, 716
BciT130I CCWGG 1 cut(s) 1079
BclI TGATCA 3 cut(s) 147, 591, 997
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 2 cut(s) 849, 1070
BfmI CTRYAG 2 cut(s) 243, 276
BmcAI AGTACT 2 cut(s) 177, 853
Bme1390I CCNGG 1 cut(s) 1079
BmrFI CCNGG 1 cut(s) 1079
BmsI GCATC 4 cut(s) 164, 813, 1031, 1111
BpiI GAAGAC 1 cut(s) 690
BpmI CTGGAG 1 cut(s) 876
Bpu10I CCTNAGC 1 cut(s) 282
BsaJI CCNNGG 3 cut(s) 947, 1029, 1077
Bse1I ACTGG 3 cut(s) 92, 820, 859
Bse3DI GCAATG 2 cut(s) 398, 970
BseBI CCWGG 1 cut(s) 1079
BseDI CCNNGG 3 cut(s) 947, 1029, 1077
BseGI GGATG 2 cut(s) 744, 1160
BseMI GCAATG 2 cut(s) 398, 970
BseMII CTCAG 4 cut(s) 137, 196, 273, 1116
BseNI ACTGG 3 cut(s) 92, 820, 859
BsgI GTGCAG 2 cut(s) 117, 126
Bsh1236I CGCG 1 cut(s) 378
Bsh1285I CGRYCG 1 cut(s) 1046
BshFI GGCC 1 cut(s) 350
BsiEI CGRYCG 1 cut(s) 1046
BsiWI CGTACG 2 cut(s) 421, 697
BsmAI GTCTC 1 cut(s) 451
BsmBI CGTCTC 1 cut(s) 451
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 1 cut(s) 350
Bsp143I GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
BspACI CCGC 5 cut(s) 237, 351, 887, 974, 1066
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 4 cut(s) 136, 195, 274, 1117
BspFNI CGCG 1 cut(s) 378
BspHI TCATGA 1 cut(s) 994
BspMAI CTGCAG 2 cut(s) 247, 280
BspPI GGATC 1 cut(s) 1089
BspQI GCTCTTC 2 cut(s) 47, 192
BsrDI GCAATG 2 cut(s) 398, 970
BsrI ACTGG 3 cut(s) 92, 820, 859
BssECI CCNNGG 3 cut(s) 947, 1029, 1077
BssMI GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
BssSI CACGAG 1 cut(s) 557
BssT1I CCWWGG 2 cut(s) 947, 1029
Bst2BI CACGAG 1 cut(s) 557
Bst2UI CCWGG 1 cut(s) 1079
Bst4CI ACNGT 2 cut(s) 223, 1044
Bst6I CTCTTC 2 cut(s) 47, 192
BstAPI GCANNNNNTGC 4 cut(s) 140, 239, 263, 386
BstC8I GCNNGC 4 cut(s) 141, 405, 522, 802
BstDEI CTNAG 7 cut(s) 123, 182, 282, 704, 842, 1049, 1125
BstF5I GGATG 2 cut(s) 744, 1160
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 2 cut(s) 294, 380
BstKTI GATC 6 cut(s) 150, 594, 875, 956, 1000, 1084
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
BstMCI CGRYCG 1 cut(s) 1046
BstNI CCWGG 1 cut(s) 1079
BstNSI RCATGY 2 cut(s) 215, 896
BstSCI CCNGG 1 cut(s) 1077
BstSFI CTRYAG 2 cut(s) 243, 276
BstUI CGCG 1 cut(s) 378
BstV2I GAAGAC 1 cut(s) 690
BsuRI GGCC 1 cut(s) 350
BtsCI GGATG 2 cut(s) 744, 1160
BtsIMutI CAGTG 4 cut(s) 143, 190, 219, 918
Cac8I GCNNGC 4 cut(s) 141, 405, 522, 802
CaiI CAGNNNCTG 2 cut(s) 263, 1025
CciI TCATGA 1 cut(s) 994
CfoI GCGC 2 cut(s) 294, 380
Csp6I GTAC 7 cut(s) 116, 127, 176, 422, 698, 852, 1053
CviAII CATG 5 cut(s) 212, 688, 893, 995, 1009
CviQI GTAC 7 cut(s) 116, 127, 176, 422, 698, 852, 1053
DdeI CTNAG 7 cut(s) 123, 182, 282, 704, 842, 1049, 1125
DpnI GATC 6 cut(s) 149, 593, 874, 955, 999, 1083
DpnII GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 2 cut(s) 47, 192
EarI CTCTTC 2 cut(s) 47, 192
Eco130I CCWWGG 2 cut(s) 947, 1029
Eco57I CTGAAG 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 1077
EcoT14I CCWWGG 2 cut(s) 947, 1029
ErhI CCWWGG 2 cut(s) 947, 1029
Esp3I CGTCTC 1 cut(s) 451
FaeI CATG 5 cut(s) 215, 691, 896, 998, 1012
FalI AAGNNNNNCTT 4 cut(s) 26, 58, 199, 231
FatI CATG 5 cut(s) 211, 687, 892, 994, 1008
FbaI TGATCA 3 cut(s) 147, 591, 997
FokI GGATG 2 cut(s) 751, 1167
FspBI CTAG 2 cut(s) 849, 1070
GlaI GCGC 2 cut(s) 293, 379
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 1 cut(s) 350
HhaI GCGC 2 cut(s) 294, 380
Hin1II CATG 5 cut(s) 215, 691, 896, 998, 1012
Hin6I GCGC 2 cut(s) 292, 378
HinP1I GCGC 2 cut(s) 292, 378
HinfI GANTC 6 cut(s) 417, 692, 794, 908, 1073, 1127
HphI GGTGA 2 cut(s) 587, 1181
Hpy188I TCNGA 2 cut(s) 52, 166
Hpy188III TCNNGA 3 cut(s) 34, 103, 995
Hpy99I CGWCG 1 cut(s) 465
HpyAV CCTTC 1 cut(s) 839
HpyCH4III ACNGT 2 cut(s) 223, 1044
HpyCH4IV ACGT 1 cut(s) 1201
HpyCH4V TGCA 9 cut(s) 134, 143, 227, 233, 245, 278, 389, 781, 963
HpyF3I CTNAG 7 cut(s) 123, 182, 282, 704, 842, 1049, 1125
HpySE526I ACGT 1 cut(s) 1201
Hsp92II CATG 5 cut(s) 215, 691, 896, 998, 1012
HspAI GCGC 2 cut(s) 292, 378
Ksp22I TGATCA 3 cut(s) 147, 591, 997
Kzo9I GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
LguI GCTCTTC 2 cut(s) 47, 192
LmnI GCTCC 2 cut(s) 540, 857
LweI GCATC 4 cut(s) 164, 813, 1031, 1111
MaeI CTAG 2 cut(s) 849, 1070
MaeII ACGT 1 cut(s) 1201
MaeIII GTNAC 1 cut(s) 1015
MalI GATC 6 cut(s) 149, 593, 874, 955, 999, 1083
MboI GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
MfeI CAATTG 2 cut(s) 228, 435
MluCI AATT 6 cut(s) 228, 360, 435, 672, 932, 985
MlyI GAGTC 2 cut(s) 411, 1136
MmeI TCCRAC 2 cut(s) 90, 522
MnlI CCTC 8 cut(s) 106, 132, 244, 277, 508, 520, 647, 709
MroXI GAANNNNTTC 1 cut(s) 41
MseI TTAA 2 cut(s) 357, 882
MslI CAYNNNNRTG 3 cut(s) 637, 1013, 1092
MspA1I CMGCKG 3 cut(s) 239, 281, 589
MspR9I CCNGG 1 cut(s) 1079
MunI CAATTG 2 cut(s) 228, 435
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 1 cut(s) 1079
MvnI CGCG 1 cut(s) 378
NdeII GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
NlaIII CATG 5 cut(s) 215, 691, 896, 998, 1012
NmuCI GTSAC 1 cut(s) 1015
NspI RCATGY 2 cut(s) 215, 896
PagI TCATGA 1 cut(s) 994
PciI ACATGT 1 cut(s) 892
PciSI GCTCTTC 2 cut(s) 47, 192
PctI GAATGC 1 cut(s) 543
PdmI GAANNNNTTC 1 cut(s) 41
PfeI GAWTC 4 cut(s) 692, 794, 908, 1073
Pfl23II CGTACG 2 cut(s) 421, 697
PflFI GACNNNGTC 1 cut(s) 1042
PleI GAGTC 2 cut(s) 411, 1135
PpsI GAGTC 2 cut(s) 411, 1135
PscI ACATGT 1 cut(s) 892
Psp1406I AACGTT 1 cut(s) 1201
Psp6I CCWGG 1 cut(s) 1077
PspGI CCWGG 1 cut(s) 1077
PspLI CGTACG 2 cut(s) 421, 697
PstI CTGCAG 2 cut(s) 247, 280
PstNI CAGNNNCTG 2 cut(s) 263, 1025
PsyI GACNNNGTC 1 cut(s) 1042
PvuII CAGCTG 2 cut(s) 281, 589
RsaI GTAC 7 cut(s) 117, 128, 177, 423, 699, 853, 1054
RsaNI GTAC 7 cut(s) 116, 127, 176, 422, 698, 852, 1053
RseI CAYNNNNRTG 3 cut(s) 637, 1013, 1092
SapI GCTCTTC 2 cut(s) 47, 192
SaqAI TTAA 2 cut(s) 357, 882
Sau3AI GATC 6 cut(s) 147, 591, 872, 953, 997, 1081
ScaI AGTACT 2 cut(s) 177, 853
SchI GAGTC 2 cut(s) 411, 1136
ScrFI CCNGG 1 cut(s) 1079
SfaNI GCATC 4 cut(s) 164, 813, 1031, 1111
SfcI CTRYAG 2 cut(s) 243, 276
SmiMI CAYNNNNRTG 3 cut(s) 637, 1013, 1092
Sse9I AATT 6 cut(s) 228, 360, 435, 672, 932, 985
SsiI CCGC 5 cut(s) 237, 351, 887, 974, 1066
SspMI CTAG 2 cut(s) 849, 1070
StyD4I CCNGG 1 cut(s) 1077
StyI CCWWGG 2 cut(s) 947, 1029
TaaI ACNGT 2 cut(s) 223, 1044
TaiI ACGT 1 cut(s) 1204
TaqI TCGA 2 cut(s) 80, 1084
TaqII GACCGA 1 cut(s) 937
TasI AATT 6 cut(s) 228, 360, 435, 672, 932, 985
TatI WGTACW 2 cut(s) 175, 851
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 4 cut(s) 692, 794, 908, 1073
Tru1I TTAA 2 cut(s) 357, 882
Tru9I TTAA 2 cut(s) 357, 882
TscAI CASTG 4 cut(s) 150, 190, 226, 918
TseFI GTSAC 1 cut(s) 1015
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 5 cut(s) 639, 755, 821, 918, 983
TspGWI ACGGA 1 cut(s) 685
TspRI CASTG 4 cut(s) 150, 190, 226, 918
Tth111I GACNNNGTC 1 cut(s) 1042
XapI RAATTY 2 cut(s) 360, 932
XceI RCATGY 2 cut(s) 215, 896
XmnI GAANNNNTTC 1 cut(s) 41
XspI CTAG 2 cut(s) 849, 1070
ZrmI AGTACT 2 cut(s) 177, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.