Rh3AG259500

all-trans-retinol 13,14-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0012790
Physical Location & Seq
Reverse (-)
28824579 .. 28826677
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG259500.1

Sequence Viewer

Length: 846 bp
ATGGCAAGGACAAAGGGCTGGTTTGGTTGGGTGAAGAGGCTCTTTACTCCAGAATTAAAGACAAACACGAAAAAGATATCAAACAAGTGGAGATGGATTTTTGGAAGACTTGAACTTCAGCACTACCCTTCTCACGCTGTGCTTACAGCATCAGAAAGAGCATTTCGTGAAGCTACAGAAGAGCAGAAAAAACATGCTTTGACTGTTGCAATAGCAACAGCGGTTGCAGCCAAGGCAGCTGTAGCTGCTGCCCAAGCTGCAGCTGAGGTTGTGCGCCTAACGGGAGCTTCACAATCTTTCCACCATTTCACAAAGTTTGACAGGAACTTGGCTGCCATTAAAATTCAAAGTGCTTATCGCTCGCATCTTGCAAGGAAAGCATTGCGAGCCTTGCGAGGACTGGTCAGGCTTCAAGCCATAGTTCGTGGTCGAGCTGTGAGACGTCAAGCACTCATATCTTTGAAGCGGTTGCCATCTAACAGAAAAGCACAGACAGAGGTTACGAAAAGGAGCATTTCTACCGTAGATGTGAGATGCAACGGTAGCGAAAAGAAGCATCTTGGCAGGCCAAAAGGGGAGTTGGATGAGAGAGAAATAACACTTTTATGCAGCAGCAGACAGCTAAGGTGGGATGACAGTCTAATTTCAAAGGAGGACATTGAAGCAATATGGCTAAGAAAGCAAGAGGCCAGAATCAAAAGAGAACGGATGAAGAAATACTCGTTCTCTCTCCGGGAAAGCCACAATGTGAAAATGTTAGAAGATTCAAATGGTAGTACTGGTGGAACGTCTGGAACTGTTAAACTGAGACATTTGAGAAATGAATATTCAATGGAAGGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0016491 GO:0031082 GO:0031083 GO:0032991 GO:0044424 GO:0044444 GO:0044445 GO:0044464 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BLOC1_2

Protein Analysis

281

Amino Acids

32.07

Weight (kDa)

10.89

Isoelectric Point (pI)

51.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 111 - 129 7.9e-06 IQ calmodulin-binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012790)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76705 AT5G03960 AT5G03960 AT5G03960
fragaria_vesca FvH4_6g24720
malus_domestica MD14G1079600.v1.1
prunus_persica Prupe.7G050600_v2.0.a1
pyrus_communis pycom12g07200 pycom12g07210
rosa_chinensis RchiOBHm_Chr3g0484271
rosa_laevigata RLG00000023235
rosa_rugosa Rorug03G0210500 Rorug03G0210500
rosa_samantha Rh3AG259500 Rh3BG296100 Rh3CG293600 Rh3DG289500
rosa_wichuraiana Rw3G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 445
AciI CCGC 2 cut(s) 221, 466
AcsI RAATTY 1 cut(s) 342
AcuI CTGAAG 1 cut(s) 101
AcyI GRCGYC 1 cut(s) 442
AdeI CACNNNGTG 2 cut(s) 139, 748
AfaI GTAC 1 cut(s) 778
AgsI TTSAA 8 cut(s) 113, 347, 413, 463, 648, 662, 768, 831
AluBI AGCT 8 cut(s) 173, 239, 245, 257, 263, 287, 434, 622
AluI AGCT 8 cut(s) 173, 239, 245, 257, 263, 287, 434, 622
Alw26I GTCTC 2 cut(s) 433, 802
AoxI GGCC 2 cut(s) 566, 687
ApeKI GCWGC 9 cut(s) 227, 236, 245, 248, 257, 260, 332, 609, 612
ApoI RAATTY 1 cut(s) 342
AspLEI GCGC 1 cut(s) 276
AsuC2I CCSGG 1 cut(s) 734
AsuHPI GGTGA 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 112
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 9 cut(s) 232, 235, 239, 244, 248, 272, 319, 621, 624
BccI CCATC 2 cut(s) 87, 481
BcnI CCSGG 1 cut(s) 734
BcoDI GTCTC 2 cut(s) 433, 802
BfmI CTRYAG 3 cut(s) 174, 240, 258
BisI GCNGC 9 cut(s) 228, 237, 246, 249, 258, 261, 333, 610, 613
BlsI GCNGC 9 cut(s) 229, 238, 247, 250, 259, 262, 334, 611, 614
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 1 cut(s) 734
BmrFI CCNGG 1 cut(s) 734
BmsI GCATC 4 cut(s) 158, 373, 524, 565
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 33
Bpu10I CCTNAGC 2 cut(s) 264, 623
BpuMI CCSGG 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 442
BsaJI CCNNGG 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 405, 784
Bse3DI GCAATG 1 cut(s) 380
BseDI CCNNGG 1 cut(s) 231
BseGI GGATG 3 cut(s) 589, 637, 714
BseMI GCAATG 1 cut(s) 380
BseMII CTCAG 2 cut(s) 255, 797
BseNI ACTGG 2 cut(s) 405, 784
BseXI GCAGC 9 cut(s) 232, 235, 239, 244, 248, 272, 319, 621, 624
BshFI GGCC 2 cut(s) 568, 689
BsiSI CCGG 1 cut(s) 733
BsmAI GTCTC 2 cut(s) 433, 802
BsmBI CGTCTC 1 cut(s) 433
BsnI GGCC 2 cut(s) 568, 689
BspACI CCGC 2 cut(s) 221, 466
BspANI GGCC 2 cut(s) 568, 689
BspCNI CTCAG 2 cut(s) 256, 798
BspMAI CTGCAG 1 cut(s) 262
BspQI GCTCTTC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 380
BsrI ACTGG 2 cut(s) 405, 784
BssECI CCNNGG 1 cut(s) 231
BssNI GRCGYC 1 cut(s) 442
BssT1I CCWWGG 1 cut(s) 231
Bst4CI ACNGT 5 cut(s) 205, 523, 542, 638, 799
Bst6I CTCTTC 2 cut(s) 29, 174
BstACI GRCGYC 1 cut(s) 442
BstC8I GCNNGC 3 cut(s) 362, 387, 566
BstDEI CTNAG 4 cut(s) 264, 623, 674, 806
BstF5I GGATG 3 cut(s) 589, 637, 714
BstHHI GCGC 1 cut(s) 276
BstMAI GTCTC 2 cut(s) 433, 802
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 732
BstSFI CTRYAG 3 cut(s) 174, 240, 258
BstV1I GCAGC 9 cut(s) 232, 235, 239, 244, 248, 272, 319, 621, 624
BstV2I GAAGAC 1 cut(s) 112
BsuRI GGCC 2 cut(s) 568, 689
BtsCI GGATG 3 cut(s) 589, 637, 714
Cac8I GCNNGC 3 cut(s) 362, 387, 566
CfoI GCGC 1 cut(s) 276
Csp6I GTAC 1 cut(s) 777
CviAII CATG 1 cut(s) 194
CviQI GTAC 1 cut(s) 777
DdeI CTNAG 4 cut(s) 264, 623, 674, 806
DraIII CACNNNGTG 2 cut(s) 139, 748
Eam1104I CTCTTC 2 cut(s) 29, 174
EarI CTCTTC 2 cut(s) 29, 174
Eco130I CCWWGG 1 cut(s) 231
Eco32I GATATC 1 cut(s) 78
Eco57I CTGAAG 1 cut(s) 101
EcoRV GATATC 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 433
FaeI CATG 1 cut(s) 197
FaiI YATR 5 cut(s) 195, 419, 455, 607, 670
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FatI CATG 1 cut(s) 193
Fnu4HI GCNGC 9 cut(s) 228, 237, 246, 249, 258, 261, 333, 610, 613
FokI GGATG 3 cut(s) 596, 644, 721
Fsp4HI GCNGC 9 cut(s) 228, 237, 246, 249, 258, 261, 333, 610, 613
GlaI GCGC 1 cut(s) 275
GluI GCNGC 9 cut(s) 228, 237, 246, 249, 258, 261, 333, 610, 613
GsuI CTGGAG 1 cut(s) 33
HaeIII GGCC 2 cut(s) 568, 689
HapII CCGG 1 cut(s) 733
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 442
Hin1II CATG 1 cut(s) 197
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 2 cut(s) 693, 764
HpaII CCGG 1 cut(s) 733
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 1 cut(s) 154
Hpy188III TCNNGA 3 cut(s) 50, 167, 792
HpyAV CCTTC 2 cut(s) 138, 830
HpyCH4III ACNGT 5 cut(s) 205, 523, 542, 638, 799
HpyCH4IV ACGT 2 cut(s) 442, 788
HpyCH4V TGCA 6 cut(s) 209, 227, 260, 371, 537, 609
HpyF3I CTNAG 4 cut(s) 264, 623, 674, 806
HpySE526I ACGT 2 cut(s) 442, 788
Hsp92I GRCGYC 1 cut(s) 442
Hsp92II CATG 1 cut(s) 197
HspAI GCGC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 174
LmnI GCTCC 2 cut(s) 284, 510
Lsp1109I GCAGC 9 cut(s) 232, 235, 239, 244, 248, 272, 319, 621, 624
LweI GCATC 4 cut(s) 158, 373, 524, 565
MaeII ACGT 2 cut(s) 442, 788
MaeIII GTNAC 1 cut(s) 499
MboII GAAGA 5 cut(s) 46, 117, 191, 724, 773
MluCI AATT 3 cut(s) 53, 342, 642
MmeI TCCRAC 1 cut(s) 561
MnlI CCTC 6 cut(s) 30, 259, 389, 490, 646, 679
MseI TTAA 3 cut(s) 56, 339, 801
MslI CAYNNNNRTG 1 cut(s) 604
MspA1I CMGCKG 3 cut(s) 221, 239, 263
MspI CCGG 1 cut(s) 733
MspR9I CCNGG 1 cut(s) 734
NciI CCSGG 1 cut(s) 734
NlaIII CATG 1 cut(s) 197
NspI RCATGY 1 cut(s) 197
PciSI GCTCTTC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 693, 764
PfoI TCCNGGA 1 cut(s) 732
PkrI GCNGC 9 cut(s) 229, 238, 247, 250, 259, 262, 334, 611, 614
PstI CTGCAG 1 cut(s) 262
PvuII CAGCTG 2 cut(s) 239, 263
RsaI GTAC 1 cut(s) 778
RsaNI GTAC 1 cut(s) 777
RseI CAYNNNNRTG 1 cut(s) 604
SapI GCTCTTC 1 cut(s) 174
SaqAI TTAA 3 cut(s) 56, 339, 801
SatI GCNGC 9 cut(s) 228, 237, 246, 249, 258, 261, 333, 610, 613
ScaI AGTACT 1 cut(s) 778
ScrFI CCNGG 1 cut(s) 734
SfaNI GCATC 4 cut(s) 158, 373, 524, 565
SfcI CTRYAG 3 cut(s) 174, 240, 258
SmiMI CAYNNNNRTG 1 cut(s) 604
Sse9I AATT 3 cut(s) 53, 342, 642
SsiI CCGC 2 cut(s) 221, 466
SspI AATATT 1 cut(s) 827
StyD4I CCNGG 1 cut(s) 732
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 5 cut(s) 205, 523, 542, 638, 799
TaiI ACGT 2 cut(s) 445, 791
TaqI TCGA 2 cut(s) 430, 841
TasI AATT 3 cut(s) 53, 342, 642
TatI WGTACW 1 cut(s) 776
TfiI GAWTC 2 cut(s) 693, 764
Tru1I TTAA 3 cut(s) 56, 339, 801
Tru9I TTAA 3 cut(s) 56, 339, 801
TseI GCWGC 9 cut(s) 227, 236, 245, 248, 257, 260, 332, 609, 612
TspDTI ATGAA 2 cut(s) 725, 837
TspGWI ACGGA 1 cut(s) 721
XapI RAATTY 1 cut(s) 342
XceI RCATGY 1 cut(s) 197
ZraI GACGTC 1 cut(s) 443
ZrmI AGTACT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.