Rorug03G0210500
NAC Family

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007682
Physical Location & Seq
Reverse (-)
18789543 .. 18795374
5832 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0210500.1

Sequence Viewer

Length: 1044 bp
ATGCTTCGTGGGTTCATTTCTCCCATTACACGATCCCCTCTCACTCTCTTACGTTTCAGGCCAAGGTTCTCTAGCTTTTATTGTCGGAGCAGAGAAATGGATCAGGGCATTTCACAACGTGAAAACCAAGTGCTTCGAGTGAAGAAGCGCGTCGCATTTCGTTTATGCGATGTGTCGAGTCACACTACTGAAGTAATGGAGATTCAAGCTGACAATCCAATATTTCATGTTCTAATCATCCCTGGAAATCCAGGTGTTGTTACATTTTATAAAGACTTTGTGGAGGCTCTATATGAGCTGCTGGGTGGAACTGCGTCTGTGACAGTTGTTGGGCATATATCACACACCAAAAAGAACTGGGAGCATGGAAGGGTTTTCTCATTACAAGCCCAAATTAATCACAAGATGGACTTCATCAAACAGGAATTGCAAAATACTGCAGTCCCTTTATTACTGGTAGGGCACTCTATTGGATCGTATATATCCATGGAAATGTTTAGGAGGTCTCCAGAACAGGTGACTTATTGTGTTGGGCTATATCCGTTTTTGGCGTTAAACGCAGAGTCCACAAAGCAGGCTCTTATTGGGAAGATTACAGAGTCCCGCATTGTATGTGTTGCCTTGAGCTTTATTGTTGCGCTCTTGGGATTGTTGCCTATATCACTTTTGAGGTTAATTGTGCTCACATTCCTTGGGAAGTCATGGTCAGCTGCTGCAGTTGAGGCTGCTTGCTTTCACTTGGTAAAGTACCACACAATGCGGAATATTCTCTTTATGGCAATGACAGAATTCCAAAAGCTTTCAGAAACACCAGATTGGGCATTCATGAGGGAAAATCTGGACAAGATAGCATTTTTGTTCGCCATTGATGATCACTGGGCTCCACTGCAAATGTTTGAAGAGATTGCCGAGCAGGTCCCTGGTGTTGCTCTTTCAATTGAACGAGGGGGTCATGCTACTCATGGTTTCTGTTGCACTGAAGCTGGCTCATTGTGGGTTGCTGAGCAGGTAGCAAGCTTGATCAAGATTAAAGTTTCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

347

Amino Acids

39.09

Weight (kDa)

8.43

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIDHydrolase PF10230 76 - 324 2.9e-68 Lipid-droplet associated hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
Acc36I ACCTGC 2 cut(s) 904, 997
AccII CGCG 1 cut(s) 150
AciI CCGC 2 cut(s) 604, 760
AclWI GGATC 3 cut(s) 27, 108, 481
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 2 cut(s) 210, 999
AdeI CACNNNGTG 1 cut(s) 119
AfaI GTAC 1 cut(s) 749
AgsI TTSAA 4 cut(s) 206, 899, 936, 941
AjnI CCWGG 3 cut(s) 241, 250, 919
AluBI AGCT 8 cut(s) 75, 209, 298, 627, 710, 799, 983, 1017
AluI AGCT 8 cut(s) 75, 209, 298, 627, 710, 799, 983, 1017
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 3 cut(s) 27, 108, 481
AlwNI CAGNNNCTG 1 cut(s) 713
AoxI GGCC 1 cut(s) 59
ApeKI GCWGC 4 cut(s) 298, 710, 713, 725
ApoI RAATTY 1 cut(s) 788
AseI ATTAAT 1 cut(s) 396
AspLEI GCGC 2 cut(s) 150, 640
AspS9I GGNCC 1 cut(s) 916
AsuHPI GGTGA 1 cut(s) 529
AvaII GGWCC 1 cut(s) 916
BaeGI GKGCMC 1 cut(s) 465
BanII GRGCYC 1 cut(s) 883
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 4 cut(s) 285, 697, 700, 712
BccI CCATC 1 cut(s) 400
BciT130I CCWGG 3 cut(s) 243, 252, 921
BclI TGATCA 2 cut(s) 871, 1020
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 1 cut(s) 72
BfmI CTRYAG 2 cut(s) 438, 714
BfuAI ACCTGC 2 cut(s) 904, 997
BisI GCNGC 4 cut(s) 299, 711, 714, 726
BlpI GCTNAGC 1 cut(s) 1002
BlsI GCNGC 4 cut(s) 300, 712, 715, 727
Bme1390I CCNGG 3 cut(s) 243, 252, 921
Bme18I GGWCC 1 cut(s) 916
BmgT120I GGNCC 1 cut(s) 916
BmiI GGNNCC 2 cut(s) 882, 918
BmrFI CCNGG 3 cut(s) 243, 252, 921
BmrI ACTGGG 2 cut(s) 367, 886
BmuI ACTGGG 2 cut(s) 367, 886
BpmI CTGGAG 1 cut(s) 492
Bpu1102I GCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 1 cut(s) 643
BsaI GGTCTC 1 cut(s) 510
BsaJI CCNNGG 5 cut(s) 62, 241, 486, 691, 919
Bse1I ACTGG 3 cut(s) 362, 459, 881
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 3 cut(s) 243, 252, 921
BseDI CCNNGG 5 cut(s) 62, 241, 486, 691, 919
BseGI GGATG 1 cut(s) 237
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 1 cut(s) 993
BseNI ACTGG 3 cut(s) 362, 459, 881
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 4 cut(s) 285, 697, 700, 712
BseYI CCCAGC 1 cut(s) 301
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 684
BslFI GGGAC 3 cut(s) 428, 586, 902
BsmAI GTCTC 1 cut(s) 510
BsmFI GGGAC 3 cut(s) 428, 586, 902
BsmI GAATGC 1 cut(s) 821
BsnI GGCC 1 cut(s) 61
Bso31I GGTCTC 1 cut(s) 510
Bsp1286I GDGCHC 3 cut(s) 465, 684, 883
Bsp143I GATC 5 cut(s) 32, 100, 473, 871, 1020
Bsp1720I GCTNAGC 1 cut(s) 1002
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 2 cut(s) 604, 760
BspANI GGCC 1 cut(s) 61
BspCNI CTCAG 1 cut(s) 994
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 2 cut(s) 882, 918
BspMAI CTGCAG 2 cut(s) 442, 718
BspMI ACCTGC 2 cut(s) 904, 997
BspPI GGATC 3 cut(s) 27, 108, 481
BspTNI GGTCTC 1 cut(s) 510
BsrDI GCAATG 1 cut(s) 786
BsrI ACTGG 3 cut(s) 362, 459, 881
BssECI CCNNGG 5 cut(s) 62, 241, 486, 691, 919
BssMI GATC 5 cut(s) 32, 100, 473, 871, 1020
BssT1I CCWWGG 3 cut(s) 62, 486, 691
Bst2UI CCWGG 3 cut(s) 243, 252, 921
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 894
BstC8I GCNNGC 4 cut(s) 576, 730, 985, 1015
BstDEI CTNAG 1 cut(s) 1002
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 1 cut(s) 237
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 2 cut(s) 150, 640
BstKTI GATC 5 cut(s) 35, 103, 476, 874, 1023
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 5 cut(s) 32, 100, 473, 871, 1020
BstMWI GCNNNNNNNGC 2 cut(s) 557, 722
BstNI CCWGG 3 cut(s) 243, 252, 921
BstSCI CCNGG 3 cut(s) 241, 250, 919
BstSFI CTRYAG 2 cut(s) 438, 714
BstSLI GKGCMC 1 cut(s) 465
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 4 cut(s) 285, 697, 700, 712
BsuRI GGCC 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 884
BtsIMutI CAGTG 3 cut(s) 874, 884, 975
BveI ACCTGC 2 cut(s) 904, 997
Cac8I GCNNGC 4 cut(s) 576, 730, 985, 1015
CaiI CAGNNNCTG 1 cut(s) 713
CciI TCATGA 1 cut(s) 825
CfoI GCGC 2 cut(s) 150, 640
Cfr13I GGNCC 1 cut(s) 916
CseI GACGC 2 cut(s) 139, 303
Csp6I GTAC 1 cut(s) 748
CviAII CATG 7 cut(s) 227, 365, 487, 702, 826, 953, 962
CviQI GTAC 1 cut(s) 748
DdeI CTNAG 1 cut(s) 1002
DpnI GATC 5 cut(s) 34, 102, 475, 873, 1022
DpnII GATC 5 cut(s) 32, 100, 473, 871, 1020
DraIII CACNNNGTG 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
Eco130I CCWWGG 3 cut(s) 62, 486, 691
Eco24I GRGCYC 1 cut(s) 883
Eco31I GGTCTC 1 cut(s) 510
Eco47I GGWCC 1 cut(s) 916
Eco57I CTGAAG 2 cut(s) 210, 999
EcoO109I RGGNCCY 1 cut(s) 916
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 3 cut(s) 241, 250, 919
EcoT14I CCWWGG 3 cut(s) 62, 486, 691
EcoT38I GRGCYC 1 cut(s) 883
ErhI CCWWGG 3 cut(s) 62, 486, 691
FaeI CATG 7 cut(s) 230, 368, 490, 705, 829, 956, 965
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 3 cut(s) 428, 586, 902
FatI CATG 7 cut(s) 226, 364, 486, 701, 825, 952, 961
FauI CCCGC 1 cut(s) 611
FbaI TGATCA 2 cut(s) 871, 1020
Fnu4HI GCNGC 4 cut(s) 299, 711, 714, 726
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 883
Fsp4HI GCNGC 4 cut(s) 299, 711, 714, 726
FspBI CTAG 1 cut(s) 72
GlaI GCGC 2 cut(s) 149, 639
GluI GCNGC 4 cut(s) 299, 711, 714, 726
GsaI CCCAGC 1 cut(s) 305
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 1 cut(s) 61
HgaI GACGC 2 cut(s) 139, 303
HhaI GCGC 2 cut(s) 150, 640
Hin1II CATG 7 cut(s) 230, 368, 490, 705, 829, 956, 965
Hin6I GCGC 2 cut(s) 148, 638
HinP1I GCGC 2 cut(s) 148, 638
HindIII AAGCTT 2 cut(s) 797, 1015
HinfI GANTC 4 cut(s) 178, 202, 563, 599
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 1 cut(s) 567
Hpy188I TCNGA 2 cut(s) 87, 805
Hpy188III TCNNGA 4 cut(s) 509, 826, 839, 1024
Hpy8I GTNNAC 1 cut(s) 567
Hpy99I CGWCG 1 cut(s) 155
HpyAV CCTTC 1 cut(s) 363
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4IV ACGT 2 cut(s) 52, 118
HpyCH4V TGCA 5 cut(s) 430, 440, 716, 889, 975
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 722
HpyF3I CTNAG 1 cut(s) 1002
HpySE526I ACGT 2 cut(s) 52, 118
Hsp92II CATG 7 cut(s) 230, 368, 490, 705, 829, 956, 965
HspAI GCGC 2 cut(s) 148, 638
Ksp22I TGATCA 2 cut(s) 871, 1020
Kzo9I GATC 5 cut(s) 32, 100, 473, 871, 1020
LmnI GCTCC 3 cut(s) 87, 361, 886
Lsp1109I GCAGC 4 cut(s) 285, 697, 700, 712
MaeI CTAG 1 cut(s) 72
MaeII ACGT 2 cut(s) 52, 118
MaeIII GTNAC 4 cut(s) 179, 259, 319, 517
MalI GATC 5 cut(s) 34, 102, 475, 873, 1022
MboI GATC 5 cut(s) 32, 100, 473, 871, 1020
MboII GAAGA 3 cut(s) 154, 601, 911
MfeI CAATTG 1 cut(s) 936
MhlI GDGCHC 3 cut(s) 465, 684, 883
MluCI AATT 5 cut(s) 393, 425, 675, 788, 936
MlyI GAGTC 3 cut(s) 187, 572, 608
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 7 cut(s) 48, 277, 495, 663, 715, 822, 938
MseI TTAA 4 cut(s) 396, 554, 674, 1029
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 710
MspR9I CCNGG 3 cut(s) 243, 252, 921
MunI CAATTG 1 cut(s) 936
Mva1269I GAATGC 1 cut(s) 821
MvaI CCWGG 3 cut(s) 243, 252, 921
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 557, 722
NcoI CCATGG 1 cut(s) 486
NdeII GATC 5 cut(s) 32, 100, 473, 871, 1020
NlaIII CATG 7 cut(s) 230, 368, 490, 705, 829, 956, 965
NlaIV GGNNCC 2 cut(s) 882, 918
NmeAIII GCCGAG 1 cut(s) 934
NmuCI GTSAC 3 cut(s) 179, 319, 517
PagI TCATGA 1 cut(s) 825
PctI GAATGC 1 cut(s) 821
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 4 cut(s) 300, 712, 715, 727
PleI GAGTC 3 cut(s) 186, 571, 607
PpsI GAGTC 3 cut(s) 186, 571, 607
PpuMI RGGWCCY 1 cut(s) 916
PshBI ATTAAT 1 cut(s) 396
PsiI TTATAA 1 cut(s) 270
Psp5II RGGWCCY 1 cut(s) 916
Psp6I CCWGG 3 cut(s) 241, 250, 919
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 3 cut(s) 241, 250, 919
PspN4I GGNNCC 2 cut(s) 882, 918
PspPI GGNCC 1 cut(s) 916
PspPPI RGGWCCY 1 cut(s) 916
PstI CTGCAG 2 cut(s) 442, 718
PstNI CAGNNNCTG 1 cut(s) 713
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 1 cut(s) 749
RsaNI GTAC 1 cut(s) 748
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 4 cut(s) 396, 554, 674, 1029
SatI GCNGC 4 cut(s) 299, 711, 714, 726
Sau3AI GATC 5 cut(s) 32, 100, 473, 871, 1020
Sau96I GGNCC 1 cut(s) 916
SchI GAGTC 3 cut(s) 187, 572, 608
ScrFI CCNGG 3 cut(s) 243, 252, 921
SduI GDGCHC 3 cut(s) 465, 684, 883
SfcI CTRYAG 2 cut(s) 438, 714
SinI GGWCC 1 cut(s) 916
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
Sse9I AATT 5 cut(s) 393, 425, 675, 788, 936
SsiI CCGC 2 cut(s) 604, 760
SspI AATATT 2 cut(s) 222, 766
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 3 cut(s) 241, 250, 919
StyI CCWWGG 3 cut(s) 62, 486, 691
TaaI ACNGT 1 cut(s) 325
TaiI ACGT 2 cut(s) 55, 121
TaqI TCGA 2 cut(s) 136, 176
TasI AATT 5 cut(s) 393, 425, 675, 788, 936
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 4 cut(s) 396, 554, 674, 1029
Tru9I TTAA 4 cut(s) 396, 554, 674, 1029
TscAI CASTG 3 cut(s) 881, 891, 982
TseFI GTSAC 3 cut(s) 179, 319, 517
TseI GCWGC 4 cut(s) 298, 710, 713, 725
Tsp45I GTSAC 3 cut(s) 179, 319, 517
TspDTI ATGAA 5 cut(s) 4, 215, 403, 814, 1026
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 3 cut(s) 881, 891, 982
VpaK11BI GGWCC 1 cut(s) 916
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 1 cut(s) 788
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.