MD14G1080200.v1.1
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007682
Physical Location & Seq
Reverse (-)
9159744 .. 9166829
7086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1080200.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGACAGAGACGGGGCAATTCTTGTTCTTCACATCATGTCTGTACATCATCATCTACTCTCTGATCATCTCCACTGTCGTCGTCGCTCTACCCACCAAGCCAGAAGGTTACGATCTACGGCCGGGTGCAGCAGATGCACCGTTCAAGATCGCTCTGTTCGCCGACCTGCATTTTGGAGAGGCCGAGTCCACCGAATGGGGCCCTCTACAGGATGTTAATTCTACTAGGGTCATGTCCTCTGTACTCGATCATGAAACCCCAGAGTTTGTTATCTATCTCGGAGATGTCATCACAGCTAATAATATATTAATTAGAAATGCAAGCTTGTATTGGGATCAGGCAATTTCTCCAACAAGAGCCAGGGGTATTCCTTGGGCCAGTGTGTTTGGGAACCATGATGATGCAGCATTTGAGTGGCCGATTGAGTGGTTTTCGGCTCCTGGAATTCCTAAAATTCATTGTCCTGTAGCCAATTCATCATGCTCAGGGAAAGAAGACTGTAGCTTCCAGGGAACTCAACGTTTGGAGATAATGAAAAATGAGATTGAGCTTAACACGCTATCACATTCTAGATATGGCCCGAAGGGGCTTTGGCCTAGTGTATCCAACTATGTCCTCCAAGTTTTGTCATCAGAAGATCCCAAGTCAGCTGTGGCATTTTTGTACTTCCTAGATTCTGGTGGTGGTTCCTACCCAGAAGTTATATCCGGCGCCCAAGCAGAATGGTTCCGGCAGAAAGCTCTAGAGATTAACCCTGATTCAAGTGTCCCAGAGATAATCTTTTGGCATATACCAAGTCGTGTGTATGAGAAAGTTGCTCCAAAGTTCGGCATACACAAGCCTTGCGTGGGTTCACTTAACGAAGAGCAAGTAGCACCTCAAGAAGCTGAAATGGGTATGATGAAACTTTTGGCCGAAAGGAAATCTGCCAAGGCAATATTTGTAGGACACAACCATGGATTAGACTGGTGCTGTCCCCATAAAGAATTGTGGCTATGTTTTGCTAGGCATACCGGTTATGGCGGTTATGGAAACTGGGATAGAGGAGCTAGAATTGTGGAAATCACTCAAAAGCCTTTCTCCGTTAAATCTTGGATAAGAATGGAGGATGGCAATGTCCATAGTGAAGTTGTCTTGAGTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

382

Amino Acids

42.49

Weight (kDa)

5.4

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 48 - 319 2e-09 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 174
AccB1I GGYRCC 1 cut(s) 710
AccB7I CCANNNNNTGG 1 cut(s) 195
AciI CCGC 1 cut(s) 1023
AclI AACGTT 1 cut(s) 520
AclWI GGATC 2 cut(s) 342, 632
AcoI YGGCCR 3 cut(s) 119, 416, 912
AcsI RAATTY 2 cut(s) 444, 453
AcyI GRCGYC 1 cut(s) 711
AfaI GTAC 3 cut(s) 44, 243, 665
AfiI CCNNNNNNNGG 4 cut(s) 195, 208, 827, 848
AgeI ACCGGT 1 cut(s) 1013
AgsI TTSAA 2 cut(s) 145, 762
AjnI CCWGG 3 cut(s) 359, 439, 507
AluBI AGCT 8 cut(s) 296, 324, 504, 550, 650, 740, 887, 1049
AluI AGCT 8 cut(s) 296, 324, 504, 550, 650, 740, 887, 1049
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 2 cut(s) 342, 632
AoxI GGCC 8 cut(s) 119, 180, 199, 375, 416, 577, 593, 912
ApaI GGGCCC 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 128, 404
ApoI RAATTY 2 cut(s) 444, 453
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
AseI ATTAAT 1 cut(s) 308
AsiGI ACCGGT 1 cut(s) 1013
AspLEI GCGC 1 cut(s) 713
AspS9I GGNCC 4 cut(s) 199, 200, 375, 578
AsuC2I CCSGG 1 cut(s) 123
BaeGI GKGCMC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 710
BanII GRGCYC 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 501
BbvI GCAGC 2 cut(s) 140, 416
BccI CCATC 1 cut(s) 1103
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 3 cut(s) 361, 441, 509
BciVI GTATCC 1 cut(s) 613
BclI TGATCA 1 cut(s) 63
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 2
BfaI CTAG 7 cut(s) 225, 570, 597, 671, 743, 1005, 1050
BfmI CTRYAG 3 cut(s) 206, 465, 499
BfoI RGCGCY 1 cut(s) 714
BfuAI ACCTGC 1 cut(s) 174
BfuI GTATCC 1 cut(s) 613
BisI GCNGC 2 cut(s) 129, 405
BlsI GCNGC 2 cut(s) 130, 406
Bme1390I CCNGG 4 cut(s) 123, 361, 441, 509
BmgT120I GGNCC 4 cut(s) 199, 200, 375, 578
BmiI GGNNCC 7 cut(s) 200, 201, 392, 438, 688, 712, 728
BmrFI CCNGG 4 cut(s) 123, 361, 441, 509
BmrI ACTGGG 1 cut(s) 1045
BmsI GCATC 2 cut(s) 124, 391
BmuI ACTGGG 1 cut(s) 1045
BpiI GAAGAC 1 cut(s) 501
Bpu10I CCTNAGC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 864
BpuMI CCSGG 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 711
BsaJI CCNNGG 5 cut(s) 360, 371, 508, 930, 955
BsaWI WCCGGW 1 cut(s) 1013
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 208, 827, 848
Bse118I RCCGGY 1 cut(s) 1013
Bse1I ACTGG 3 cut(s) 378, 971, 1040
Bse3DI GCAATG 1 cut(s) 1120
BseBI CCWGG 3 cut(s) 361, 441, 509
BseDI CCNNGG 5 cut(s) 360, 371, 508, 930, 955
BseGI GGATG 2 cut(s) 217, 1114
BseLI CCNNNNNNNGG 4 cut(s) 195, 208, 827, 848
BseMI GCAATG 1 cut(s) 1120
BseMII CTCAG 1 cut(s) 498
BseNI ACTGG 3 cut(s) 378, 971, 1040
BseRI GAGGAG 1 cut(s) 1059
BseSI GKGCMC 1 cut(s) 203
BseX3I CGGCCG 1 cut(s) 119
BseXI GCAGC 2 cut(s) 140, 416
BsgI GTGCAG 1 cut(s) 147
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 8 cut(s) 121, 182, 201, 377, 418, 579, 595, 914
BshNI GGYRCC 1 cut(s) 710
BshTI ACCGGT 1 cut(s) 1013
BsiEI CGRYCG 1 cut(s) 122
BsiSI CCGG 4 cut(s) 122, 708, 730, 1014
BslFI GGGAC 3 cut(s) 752, 960, 1125
BslI CCNNNNNNNGG 4 cut(s) 195, 208, 827, 848
BsmAI GTCTC 1 cut(s) 2
BsmBI CGTCTC 1 cut(s) 2
BsmFI GGGAC 3 cut(s) 752, 960, 1125
BsnI GGCC 8 cut(s) 121, 182, 201, 377, 418, 579, 595, 914
Bsp120I GGGCCC 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 203
Bsp1407I TGTACA 1 cut(s) 42
Bsp143I GATC 6 cut(s) 63, 112, 147, 247, 334, 637
Bsp19I CCATGG 1 cut(s) 955
BspACI CCGC 1 cut(s) 1023
BspANI GGCC 8 cut(s) 121, 182, 201, 377, 418, 579, 595, 914
BspCNI CTCAG 1 cut(s) 497
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 7 cut(s) 200, 201, 392, 438, 688, 712, 728
BspMI ACCTGC 1 cut(s) 174
BspPI GGATC 2 cut(s) 342, 632
BspQI GCTCTTC 1 cut(s) 858
BspT107I GGYRCC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 1120
BsrFI RCCGGY 1 cut(s) 1013
BsrGI TGTACA 1 cut(s) 42
BsrI ACTGG 3 cut(s) 378, 971, 1040
BssAI RCCGGY 1 cut(s) 1013
BssECI CCNNGG 5 cut(s) 360, 371, 508, 930, 955
BssMI GATC 6 cut(s) 63, 112, 147, 247, 334, 637
BssNI GRCGYC 1 cut(s) 711
BssT1I CCWWGG 3 cut(s) 371, 930, 955
Bst2UI CCWGG 3 cut(s) 361, 441, 509
Bst4CI ACNGT 3 cut(s) 76, 141, 500
Bst6I CTCTTC 1 cut(s) 858
BstACI GRCGYC 1 cut(s) 711
BstAPI GCANNNNNTGC 1 cut(s) 134
BstAUI TGTACA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 1 cut(s) 484
BstDSI CCRYGG 1 cut(s) 955
BstF5I GGATG 2 cut(s) 217, 1114
BstH2I RGCGCY 1 cut(s) 714
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 6 cut(s) 66, 115, 150, 250, 337, 640
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 6 cut(s) 63, 112, 147, 247, 334, 637
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 3 cut(s) 134, 158, 556
BstNI CCWGG 3 cut(s) 361, 441, 509
BstSCI CCNGG 4 cut(s) 121, 359, 439, 507
BstSFI CTRYAG 3 cut(s) 206, 465, 499
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 140, 416
BstV2I GAAGAC 1 cut(s) 501
BstX2I RGATCY 1 cut(s) 637
BstYI RGATCY 1 cut(s) 637
BstZI CGGCCG 1 cut(s) 119
BsuI GTATCC 1 cut(s) 613
BsuRI GGCC 8 cut(s) 121, 182, 201, 377, 418, 579, 595, 914
BtgI CCRYGG 1 cut(s) 955
BtsCI GGATG 2 cut(s) 217, 1114
BtsIMutI CAGTG 2 cut(s) 72, 385
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 322
CciI TCATGA 1 cut(s) 250
CfoI GCGC 1 cut(s) 713
Cfr10I RCCGGY 1 cut(s) 1013
Cfr13I GGNCC 4 cut(s) 199, 200, 375, 578
Csp6I GTAC 3 cut(s) 43, 242, 664
CspAI ACCGGT 1 cut(s) 1013
CviAII CATG 6 cut(s) 36, 232, 251, 395, 480, 956
CviQI GTAC 3 cut(s) 43, 242, 664
DdeI CTNAG 1 cut(s) 484
DinI GGCGCC 1 cut(s) 712
DpnI GATC 6 cut(s) 65, 114, 149, 249, 336, 639
DpnII GATC 6 cut(s) 63, 112, 147, 247, 334, 637
EaeI YGGCCR 3 cut(s) 119, 416, 912
EagI CGGCCG 1 cut(s) 119
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
EclXI CGGCCG 1 cut(s) 119
Eco130I CCWWGG 3 cut(s) 371, 930, 955
Eco24I GRGCYC 1 cut(s) 203
Eco52I CGGCCG 1 cut(s) 119
EcoO109I RGGNCCY 2 cut(s) 199, 200
EcoRI GAATTC 1 cut(s) 444
EcoRII CCWGG 3 cut(s) 359, 439, 507
EcoT14I CCWWGG 3 cut(s) 371, 930, 955
EcoT38I GRGCYC 1 cut(s) 203
EgeI GGCGCC 1 cut(s) 712
EheI GGCGCC 1 cut(s) 712
ErhI CCWWGG 3 cut(s) 371, 930, 955
Esp3I CGTCTC 1 cut(s) 2
FaeI CATG 6 cut(s) 39, 235, 254, 398, 483, 959
FaqI GGGAC 3 cut(s) 752, 960, 1125
FatI CATG 6 cut(s) 35, 231, 250, 394, 479, 955
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 129, 405
FokI GGATG 2 cut(s) 224, 1121
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 129, 405
FspBI CTAG 7 cut(s) 225, 570, 597, 671, 743, 1005, 1050
GlaI GCGC 1 cut(s) 712
GluI GCNGC 2 cut(s) 129, 405
HaeII RGCGCY 1 cut(s) 714
HaeIII GGCC 8 cut(s) 121, 182, 201, 377, 418, 579, 595, 914
HapII CCGG 4 cut(s) 122, 708, 730, 1014
HhaI GCGC 1 cut(s) 713
Hin1I GRCGYC 1 cut(s) 711
Hin1II CATG 6 cut(s) 39, 235, 254, 398, 483, 959
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 4 cut(s) 185, 674, 758, 1138
HpaII CCGG 4 cut(s) 122, 708, 730, 1014
Hpy166II GTNNAC 2 cut(s) 189, 854
Hpy188I TCNGA 3 cut(s) 63, 281, 634
Hpy188III TCNNGA 6 cut(s) 145, 251, 570, 743, 881, 1135
Hpy8I GTNNAC 2 cut(s) 189, 854
Hpy99I CGWCG 2 cut(s) 83, 86
HpyAV CCTTC 2 cut(s) 98, 577
HpyCH4III ACNGT 3 cut(s) 76, 141, 500
HpyCH4IV ACGT 1 cut(s) 520
HpyCH4V TGCA 5 cut(s) 128, 137, 169, 320, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 158, 556
HpyF3I CTNAG 1 cut(s) 484
HpySE526I ACGT 1 cut(s) 520
Hsp92I GRCGYC 1 cut(s) 711
Hsp92II CATG 6 cut(s) 39, 235, 254, 398, 483, 959
HspAI GCGC 1 cut(s) 711
KasI GGCGCC 1 cut(s) 710
Ksp22I TGATCA 1 cut(s) 63
Kzo9I GATC 6 cut(s) 63, 112, 147, 247, 334, 637
LguI GCTCTTC 1 cut(s) 858
LmnI GCTCC 3 cut(s) 442, 823, 1046
Lsp1109I GCAGC 2 cut(s) 140, 416
LweI GCATC 2 cut(s) 124, 391
MaeI CTAG 7 cut(s) 225, 570, 597, 671, 743, 1005, 1050
MaeII ACGT 1 cut(s) 520
MaeIII GTNAC 1 cut(s) 107
MalI GATC 6 cut(s) 65, 114, 149, 249, 336, 639
MboI GATC 6 cut(s) 63, 112, 147, 247, 334, 637
MboII GAAGA 4 cut(s) 19, 506, 647, 875
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 9 cut(s) 17, 217, 309, 342, 444, 453, 472, 986, 1053
Mly113I GGCGCC 1 cut(s) 711
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 2 cut(s) 374, 630
MnlI CCTC 7 cut(s) 172, 213, 247, 626, 888, 1037, 1099
MseI TTAA 6 cut(s) 216, 308, 552, 750, 858, 1086
MslI CAYNNNNRTG 3 cut(s) 399, 412, 954
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 4 cut(s) 122, 708, 730, 1014
MspR9I CCNGG 4 cut(s) 123, 361, 441, 509
MvaI CCWGG 3 cut(s) 361, 441, 509
MwoI GCNNNNNNNGC 3 cut(s) 134, 158, 556
NarI GGCGCC 1 cut(s) 711
NciI CCSGG 1 cut(s) 123
NcoI CCATGG 1 cut(s) 955
NdeII GATC 6 cut(s) 63, 112, 147, 247, 334, 637
NlaIII CATG 6 cut(s) 39, 235, 254, 398, 483, 959
NlaIV GGNNCC 7 cut(s) 200, 201, 392, 438, 688, 712, 728
NmeAIII GCCGAG 1 cut(s) 208
PagI TCATGA 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 858
PfeI GAWTC 2 cut(s) 674, 758
PflMI CCANNNNNTGG 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 439
PinAI ACCGGT 1 cut(s) 1013
PkrI GCNGC 2 cut(s) 130, 406
PleI GAGTC 1 cut(s) 193
PluTI GGCGCC 1 cut(s) 714
PpsI GAGTC 1 cut(s) 193
PshBI ATTAAT 1 cut(s) 308
Psp1406I AACGTT 1 cut(s) 520
Psp6I CCWGG 3 cut(s) 359, 439, 507
PspGI CCWGG 3 cut(s) 359, 439, 507
PspN4I GGNNCC 7 cut(s) 200, 201, 392, 438, 688, 712, 728
PspOMI GGGCCC 1 cut(s) 199
PspPI GGNCC 4 cut(s) 199, 200, 375, 578
PsuI RGATCY 1 cut(s) 637
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 3 cut(s) 44, 243, 665
RsaNI GTAC 3 cut(s) 43, 242, 664
RseI CAYNNNNRTG 3 cut(s) 399, 412, 954
SapI GCTCTTC 1 cut(s) 858
SaqAI TTAA 6 cut(s) 216, 308, 552, 750, 858, 1086
SatI GCNGC 2 cut(s) 129, 405
Sau3AI GATC 6 cut(s) 63, 112, 147, 247, 334, 637
Sau96I GGNCC 4 cut(s) 199, 200, 375, 578
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 4 cut(s) 123, 361, 441, 509
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 2 cut(s) 124, 391
SfcI CTRYAG 3 cut(s) 206, 465, 499
SfoI GGCGCC 1 cut(s) 712
SmiMI CAYNNNNRTG 3 cut(s) 399, 412, 954
SmlI CTYRAG 2 cut(s) 879, 1135
SmoI CTYRAG 2 cut(s) 879, 1135
Sse9I AATT 9 cut(s) 17, 217, 309, 342, 444, 453, 472, 986, 1053
SsiI CCGC 1 cut(s) 1023
SspDI GGCGCC 1 cut(s) 710
SspI AATATT 1 cut(s) 939
SspMI CTAG 7 cut(s) 225, 570, 597, 671, 743, 1005, 1050
StyD4I CCNGG 4 cut(s) 121, 359, 439, 507
StyI CCWWGG 3 cut(s) 371, 930, 955
TaaI ACNGT 3 cut(s) 76, 141, 500
TaiI ACGT 1 cut(s) 523
TaqI TCGA 1 cut(s) 246
TasI AATT 9 cut(s) 17, 217, 309, 342, 444, 453, 472, 986, 1053
TatI WGTACW 3 cut(s) 42, 241, 663
TfiI GAWTC 2 cut(s) 674, 758
Tru1I TTAA 6 cut(s) 216, 308, 552, 750, 858, 1086
Tru9I TTAA 6 cut(s) 216, 308, 552, 750, 858, 1086
TscAI CASTG 2 cut(s) 79, 385
TseI GCWGC 2 cut(s) 128, 404
TspDTI ATGAA 5 cut(s) 267, 446, 465, 548, 917
TspGWI ACGGA 1 cut(s) 1072
TspRI CASTG 2 cut(s) 79, 385
Van91I CCANNNNNTGG 1 cut(s) 195
VspI ATTAAT 1 cut(s) 308
XapI RAATTY 2 cut(s) 444, 453
XbaI TCTAGA 2 cut(s) 569, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XspI CTAG 7 cut(s) 225, 570, 597, 671, 743, 1005, 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.