AT3G10150

purple acid phosphatase 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007682
Physical Location & Seq
Reverse (-)
3137362 .. 3139169
1808 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10150.2

Sequence Viewer

Length: 1104 bp
ATGAAAAAACCGTCGTTGTTTCAGATCATTATTATAGTGCTAAGCATACCAACGACTACGGGGAGGACGGTGGGAAATCTTCGGGTGCGGGAAGGATCACCTTTCAAGATCGCAATATTTGCAGACCTCCACTTCGGTGAAGACACGTGGACTGATTGGGGTCCTGGTCAGGATGTGAACTCCGTTAACGTTATGTCCGCCGTTCTCGACGCTGAAACTCCAGATTTTGTGGTCTATTTGGGAGATGTTGTGACGGCTAACAACATAGCAATCCAAAACGCAAGCTTGTTTTGGGACAAAGCAATCTCTCCTACAAGAGACAGAGGCATTCCATGGGCTACTCTCTTTGGTAACCATGACGATGCTTCTTTTGTTTGGCCTTTGGATTGGTTGTCTTCCTCCGGTATTCCTCCCCTTCGTTGCCCTGCAGCATCAGATGATGATGGATGCACGTTCAGAGGAACTACGCGAGTGGAACTGATTCAGGAAGAAATAAAGTCATCCAATGCACTCTCATACTCCATGATTAGTCCTAAGGAGCTATGGCCAAGCGTATCTAACTATGTCCTTCTTGTGGAATCATCGGATCATTCGAAACCACCCGTGGCATTGCTATATTTTCTTGATTCGGGCGGTGGTTCATACCCGGAGGTTATCTCAAATGCTCAAGTGGAATGGTTTAAAACCAAGTCTAATACTCTTAATCCCTATTTAAGGATCCCGGAGTTGATATTTTGGCACATACCTAGTAAAGCATACAAGAAAGTAGCTCCAAGGTTATGGATAACAAAACCTTGCGTTGGATCAATCAACAAAGAGAAAGTTGTTGCTCAAGAAGCTGAAAATGGGATGATGAGAGTTCTTGAGAACAGATCCTCCGTTAAGGCAGTGTTTGTAGGACACAACCATGGACTAGACTGGTGCTGTCCGTACAAAGATAAACTCTGGCTTTGCTTTGCGAGACACACAGGTTATGGTGGATATGGAAATTGGCCAAGGGGATCAAGGATTTTGGAGATTTCTGAAATGCCCTTTCGTATTAAGACCTGGATTAGGATGGAAGATGGATCTGTCCACAGTGAAGTTAACCTAACATATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0005575 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

367

Amino Acids

41.16

Weight (kDa)

6.09

Isoelectric Point (pI)

31.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 57 - 325 2.9e-11 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 469
AciI CCGC 3 cut(s) 88, 198, 633
AclI AACGTT 1 cut(s) 189
AclWI GGATC 8 cut(s) 103, 594, 712, 725, 811, 867, 1009, 1075
AcoI YGGCCR 2 cut(s) 545, 992
AcvI CACGTG 1 cut(s) 147
AfaI GTAC 1 cut(s) 932
AfiI CCNNNNNNNGG 4 cut(s) 574, 714, 800, 1053
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 2 cut(s) 163, 1046
AleI CACNNNNGTG 1 cut(s) 135
AloI GAACNNNNNNTCC 2 cut(s) 860, 892
AluBI AGCT 4 cut(s) 285, 541, 770, 839
AluI AGCT 4 cut(s) 285, 541, 770, 839
Alw26I GTCTC 2 cut(s) 312, 955
AlwI GGATC 8 cut(s) 103, 594, 712, 725, 811, 867, 1009, 1075
AoxI GGCC 3 cut(s) 377, 545, 992
ApeKI GCWGC 1 cut(s) 428
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
Asp700I GAANNNNTTC 1 cut(s) 480
AspS9I GGNCC 1 cut(s) 161
AsuC2I CCSGG 2 cut(s) 647, 722
AsuHPI GGTGA 2 cut(s) 90, 149
AsuII TTCGAA 1 cut(s) 593
AvaII GGWCC 1 cut(s) 161
AxyI CCTNAGG 1 cut(s) 534
BalI TGGCCA 2 cut(s) 547, 994
BamHI GGATCC 1 cut(s) 717
BbrPI CACGTG 1 cut(s) 147
BbsI GAAGAC 2 cut(s) 147, 387
BbvI GCAGC 1 cut(s) 440
BccI CCATC 3 cut(s) 437, 1051, 1058
BceAI ACGGC 2 cut(s) 185, 270
BciT130I CCWGG 2 cut(s) 165, 1048
BcnI CCSGG 2 cut(s) 647, 722
BcoDI GTCTC 2 cut(s) 312, 955
BfaI CTAG 2 cut(s) 747, 914
BfmI CTRYAG 1 cut(s) 426
BisI GCNGC 1 cut(s) 429
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 4 cut(s) 165, 647, 722, 1048
Bme18I GGWCC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 161
BmiI GGNNCC 2 cut(s) 162, 719
BmrFI CCNGG 4 cut(s) 165, 647, 722, 1048
BmsI GCATC 3 cut(s) 352, 437, 440
BpiI GAAGAC 2 cut(s) 147, 387
BplI GAGNNNNNCTC 2 cut(s) 641, 673
BpmI CTGGAG 1 cut(s) 204
Bpu1102I GCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 593
BpuEI CTTGAG 3 cut(s) 651, 816, 884
BpuMI CCSGG 2 cut(s) 647, 722
BsaAI YACGTR 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 332, 603, 773, 907, 995
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 4 cut(s) 234, 264, 860, 890
Bsc4I CCNNNNNNNGG 4 cut(s) 574, 714, 800, 1053
Bse1I ACTGG 1 cut(s) 923
Bse21I CCTNAGG 1 cut(s) 534
Bse3DI GCAATG 1 cut(s) 608
BseBI CCWGG 2 cut(s) 165, 1048
BseDI CCNNGG 5 cut(s) 332, 603, 773, 907, 995
BseGI GGATG 5 cut(s) 178, 452, 500, 855, 1062
BseLI CCNNNNNNNGG 4 cut(s) 574, 714, 800, 1053
BseMI GCAATG 1 cut(s) 608
BseNI ACTGG 1 cut(s) 923
BseXI GCAGC 1 cut(s) 440
Bsh1236I CGCG 1 cut(s) 469
BshFI GGCC 3 cut(s) 379, 547, 994
BsiSI CCGG 3 cut(s) 402, 647, 722
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 4 cut(s) 574, 714, 800, 1053
BsmAI GTCTC 2 cut(s) 312, 955
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 3 cut(s) 379, 547, 994
Bsp119I TTCGAA 1 cut(s) 593
Bsp143I GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
Bsp1720I GCTNAGC 1 cut(s) 41
Bsp19I CCATGG 2 cut(s) 332, 907
BspACI CCGC 3 cut(s) 88, 198, 633
BspANI GGCC 3 cut(s) 379, 547, 994
BspFNI CGCG 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 162, 719
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 8 cut(s) 103, 594, 712, 725, 811, 867, 1009, 1075
BspT104I TTCGAA 1 cut(s) 593
BsrDI GCAATG 1 cut(s) 608
BsrI ACTGG 1 cut(s) 923
BssECI CCNNGG 5 cut(s) 332, 603, 773, 907, 995
BssMI GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
BssT1I CCWWGG 4 cut(s) 332, 773, 907, 995
Bst2UI CCWGG 2 cut(s) 165, 1048
Bst4CI ACNGT 3 cut(s) 12, 70, 1079
BstAPI GCANNNNNTGC 1 cut(s) 119
BstBAI YACGTR 1 cut(s) 147
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 2 cut(s) 41, 534
BstDSI CCRYGG 3 cut(s) 332, 603, 907
BstEII GGTNACC 1 cut(s) 350
BstENI CCTNNNNNAGG 2 cut(s) 712, 1051
BstF5I GGATG 5 cut(s) 178, 452, 500, 855, 1062
BstFNI CGCG 1 cut(s) 469
BstKTI GATC 9 cut(s) 27, 98, 111, 589, 720, 806, 875, 1004, 1070
BstMAI GTCTC 2 cut(s) 312, 955
BstMBI GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
BstMWI GCNNNNNNNGC 2 cut(s) 119, 836
BstNI CCWGG 2 cut(s) 165, 1048
BstPI GGTNACC 1 cut(s) 350
BstSCI CCNGG 4 cut(s) 163, 645, 720, 1046
BstSFI CTRYAG 1 cut(s) 426
BstUI CGCG 1 cut(s) 469
BstV1I GCAGC 1 cut(s) 440
BstV2I GAAGAC 2 cut(s) 147, 387
BstX2I RGATCY 3 cut(s) 717, 872, 1067
BstXI CCANNNNNNTGG 1 cut(s) 780
BstYI RGATCY 3 cut(s) 717, 872, 1067
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 3 cut(s) 379, 547, 994
BtgI CCRYGG 3 cut(s) 332, 603, 907
BtsCI GGATG 5 cut(s) 178, 452, 500, 855, 1062
BtsI GCAGTG 1 cut(s) 894
BtsIMutI CAGTG 2 cut(s) 894, 1084
Cac8I GCNNGC 1 cut(s) 283
Cfr13I GGNCC 1 cut(s) 161
CseI GACGC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 931
CviAII CATG 4 cut(s) 333, 356, 523, 908
CviQI GTAC 1 cut(s) 931
DdeI CTNAG 2 cut(s) 41, 534
DpnI GATC 9 cut(s) 26, 97, 110, 588, 719, 805, 874, 1003, 1069
DpnII GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
DraI TTTAAA 1 cut(s) 682
EaeI YGGCCR 2 cut(s) 545, 992
EciI GGCGGA 1 cut(s) 187
Eco130I CCWWGG 4 cut(s) 332, 773, 907, 995
Eco47I GGWCC 1 cut(s) 161
Eco72I CACGTG 1 cut(s) 147
Eco81I CCTNAGG 1 cut(s) 534
Eco91I GGTNACC 1 cut(s) 350
EcoNI CCTNNNNNAGG 2 cut(s) 712, 1051
EcoO109I RGGNCCY 1 cut(s) 161
EcoO65I GGTNACC 1 cut(s) 350
EcoRII CCWGG 2 cut(s) 163, 1046
EcoT14I CCWWGG 4 cut(s) 332, 773, 907, 995
ErhI CCWWGG 4 cut(s) 332, 773, 907, 995
FaeI CATG 4 cut(s) 336, 359, 526, 911
FaqI GGGAC 1 cut(s) 308
FatI CATG 4 cut(s) 332, 355, 522, 907
FauI CCCGC 1 cut(s) 81
FauNDI CATATG 1 cut(s) 1096
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 5 cut(s) 185, 459, 487, 862, 1069
Fsp4HI GCNGC 1 cut(s) 429
FspBI CTAG 2 cut(s) 747, 914
GluI GCNGC 1 cut(s) 429
GsuI CTGGAG 1 cut(s) 204
HaeIII GGCC 3 cut(s) 379, 547, 994
HapII CCGG 3 cut(s) 402, 647, 722
HgaI GACGC 1 cut(s) 218
Hin1II CATG 4 cut(s) 336, 359, 526, 911
HincII GTYRAC 2 cut(s) 187, 1087
HindII GTYRAC 2 cut(s) 187, 1087
HindIII AAGCTT 1 cut(s) 283
HinfI GANTC 3 cut(s) 481, 578, 626
HpaI GTTAAC 2 cut(s) 187, 1087
HpaII CCGG 3 cut(s) 402, 647, 722
HphI GGTGA 2 cut(s) 90, 149
Hpy166II GTNNAC 5 cut(s) 150, 178, 187, 1075, 1087
Hpy188I TCNGA 5 cut(s) 24, 436, 458, 586, 1024
Hpy188III TCNNGA 8 cut(s) 106, 170, 206, 221, 485, 623, 833, 863
Hpy8I GTNNAC 5 cut(s) 150, 178, 187, 1075, 1087
Hpy99I CGWCG 2 cut(s) 16, 212
HpyAV CCTTC 3 cut(s) 86, 425, 578
HpyCH4III ACNGT 3 cut(s) 12, 70, 1079
HpyCH4IV ACGT 3 cut(s) 146, 189, 452
HpyCH4V TGCA 4 cut(s) 122, 428, 450, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 836
HpyF3I CTNAG 2 cut(s) 41, 534
HpySE526I ACGT 3 cut(s) 146, 189, 452
Hsp92II CATG 4 cut(s) 336, 359, 526, 911
KspAI GTTAAC 2 cut(s) 187, 1087
Kzo9I GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
LmnI GCTCC 2 cut(s) 538, 775
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 3 cut(s) 352, 437, 440
MaeI CTAG 2 cut(s) 747, 914
MaeII ACGT 3 cut(s) 146, 189, 452
MaeIII GTNAC 2 cut(s) 250, 350
MalI GATC 9 cut(s) 26, 97, 110, 588, 719, 805, 874, 1003, 1069
MboI GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
MboII GAAGA 5 cut(s) 71, 152, 387, 500, 1073
MflI RGATCY 3 cut(s) 717, 872, 1067
MlsI TGGCCA 2 cut(s) 547, 994
MluCI AATT 1 cut(s) 988
MluNI TGGCCA 2 cut(s) 547, 994
MmeI TCCRAC 1 cut(s) 781
MnlI CCTC 8 cut(s) 57, 137, 317, 409, 420, 452, 643, 886
Mox20I TGGCCA 2 cut(s) 547, 994
MroXI GAANNNNTTC 1 cut(s) 480
MscI TGGCCA 2 cut(s) 547, 994
MseI TTAA 7 cut(s) 186, 681, 702, 713, 882, 1041, 1086
MslI CAYNNNNRTG 3 cut(s) 135, 360, 906
Msp20I TGGCCA 2 cut(s) 547, 994
MspI CCGG 3 cut(s) 402, 647, 722
MspR9I CCNGG 4 cut(s) 165, 647, 722, 1048
Mva1269I GAATGC 1 cut(s) 327
MvaI CCWGG 2 cut(s) 165, 1048
MvnI CGCG 1 cut(s) 469
MwoI GCNNNNNNNGC 2 cut(s) 119, 836
NciI CCSGG 2 cut(s) 647, 722
NcoI CCATGG 2 cut(s) 332, 907
NdeI CATATG 1 cut(s) 1096
NdeII GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
NlaIII CATG 4 cut(s) 336, 359, 526, 911
NlaIV GGNNCC 2 cut(s) 162, 719
NmuCI GTSAC 1 cut(s) 250
NspV TTCGAA 1 cut(s) 593
OliI CACNNNNGTG 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 590
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 3 cut(s) 481, 578, 626
PfoI TCCNGGA 1 cut(s) 720
PkrI GCNGC 1 cut(s) 430
PmaCI CACGTG 1 cut(s) 147
PmlI CACGTG 1 cut(s) 147
Ppu21I YACGTR 1 cut(s) 147
PpuMI RGGWCCY 1 cut(s) 161
Psp1406I AACGTT 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 2 cut(s) 163, 1046
PspCI CACGTG 1 cut(s) 147
PspEI GGTNACC 1 cut(s) 350
PspGI CCWGG 2 cut(s) 163, 1046
PspN4I GGNNCC 2 cut(s) 162, 719
PspPI GGNCC 1 cut(s) 161
PspPPI RGGWCCY 1 cut(s) 161
PstI CTGCAG 1 cut(s) 430
PsuI RGATCY 3 cut(s) 717, 872, 1067
RsaI GTAC 1 cut(s) 932
RsaNI GTAC 1 cut(s) 931
RseI CAYNNNNRTG 3 cut(s) 135, 360, 906
SaqAI TTAA 7 cut(s) 186, 681, 702, 713, 882, 1041, 1086
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 9 cut(s) 24, 95, 108, 586, 717, 803, 872, 1001, 1067
Sau96I GGNCC 1 cut(s) 161
ScrFI CCNGG 4 cut(s) 165, 647, 722, 1048
SfaNI GCATC 3 cut(s) 352, 437, 440
SfcI CTRYAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 593
SinI GGWCC 1 cut(s) 161
SmiMI CAYNNNNRTG 3 cut(s) 135, 360, 906
SmlI CTYRAG 3 cut(s) 666, 831, 863
SmoI CTYRAG 3 cut(s) 666, 831, 863
Sse9I AATT 1 cut(s) 988
SsiI CCGC 3 cut(s) 88, 198, 633
SspI AATATT 1 cut(s) 117
SspMI CTAG 2 cut(s) 747, 914
StyD4I CCNGG 4 cut(s) 163, 645, 720, 1046
StyI CCWWGG 4 cut(s) 332, 773, 907, 995
TaaI ACNGT 3 cut(s) 12, 70, 1079
TaiI ACGT 3 cut(s) 149, 192, 455
TaqI TCGA 2 cut(s) 207, 593
TasI AATT 1 cut(s) 988
TfiI GAWTC 3 cut(s) 481, 578, 626
Tru1I TTAA 7 cut(s) 186, 681, 702, 713, 882, 1041, 1086
Tru9I TTAA 7 cut(s) 186, 681, 702, 713, 882, 1041, 1086
TscAI CASTG 2 cut(s) 894, 1084
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 17, 630
TspGWI ACGGA 3 cut(s) 172, 868, 918
TspRI CASTG 2 cut(s) 894, 1084
VpaK11BI GGWCC 1 cut(s) 161
XagI CCTNNNNNAGG 2 cut(s) 712, 1051
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 2 cut(s) 747, 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.